lh750 host transmission specifications

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  • 5/23/2018 LH750 Host Transmission Specifications

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    COULTER LH Series WorkStation

    Host Transmissio

    Specification

    PN 4277303EC (January 2013)

    Manufactured byBeckman Coulter, Inc.

    250 S. Kraemer Blvd.Brea, CA 92821 U.S.A.

    NS

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    WARNINGS AND PRECAUTIONS

    READ ALL PRODUCT MANUALS AND CONSULT WITH BECKMAN COULTER-TRAINED PERSONNEL BEFORE ATTEMPTINGTO OPERATE INSTRUMENT. DO NOT ATTEMPT TO PERFORM ANY PROCEDURE BEFORE CAREFULLY READING ALLINSTRUCTIONS. ALWAYS FOLLOW PRODUCT LABELING AND MANUFACTURERS RECOMMENDATIONS. IF IN DOUBT ASTO HOW TO PROCEED IN ANY SITUATION, CONTACT YOUR BECKMAN COULTER REPRESENTATIVE.

    BECKMAN COULTER, INC. URGES ITS CUSTOMERS TO COMPLY WITH ALL NATIONAL HEALTH AND SAFETYSTANDARDS SUCH AS THE USE OF BARRIER PROTECTION. THIS MAY INCLUDE, BUT IT IS NOT LIMITED TO,PROTECTIVE EYEWEAR, GLOVES, AND SUITABLE LABORATORY ATTIRE WHEN OPERATING OR MAINTAINING THIS ORANY OTHER AUTOMATED LABORATORY ANALYZER.

    HAZARDS AND OPERATIONAL PRECAUTIONS AND LIMITATIONS

    WARNINGS, CAUTIONS, and IMPORTANTS alert you as follows:

    WARNING - Can cause injury.

    CAUTION - Can cause damage to the instrument.

    IMPORTANT - Can cause misleading results.

    WARNING Risk of operator injury if:

    r

    All doors, covers and panels are not closed and secured in place prior to and during instrument operation.r The integrity of safety interlocks and sensors is compromised.

    r Instrument alarms and error messages are not acknowledged and acted upon.

    r You contact moving parts.

    r You mishandle broken parts.

    r Doors, covers and panels are not opened, closed, removed and/or replaced with care.

    r Improper tools are used for troubleshooting.

    To avoid injury:

    r Keep doors, covers and panels closed and secured in place while the instrument is in use.

    r Take full advantage of the safety features of the instrument. Do not defeat safety interlocks and sensors.

    r

    Acknowledge and act upon instrument alarms and error messages.r Keep away from moving parts.

    r Report any broken parts to your Beckman Coulter Representative.

    r Open/remove and close/replace doors, covers and panels with care.

    r Use the proper tools when troubleshooting.

    CAUTION System integrity might be compromised and operational failures might occur if:

    r This equipment is used in a manner other than specified. Operate the instrument as instructed in the Product Manuals.

    r You introduce software that is not authorized by Beckman Coulter into your computer. Only operate your systemscomputer with software authorized by Beckman Coulter.

    r You install software that is not an original copyrighted version. Only use software that is an original copyrightedversion to prevent virus contamination.

    IMPORTANT If you purchased this product from anyone other than Beckman Coulter or an authorized Beckman Coulterdistributor, and, if it is not presently under a Beckman Coulter service maintenance agreement, Beckman Coulter cannotguarantee that the product is fitted with the most current mandatory engineering revisions or that you will receive the mostcurrent information bulletins concerning the product. If you purchased this product from a third party and would likefurther information concerning this topic, call your Beckman Coulter Representative.

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    REVISION STATUS

    Initial Issue, 7/01Software Version 1A.

    Revision B, 4/02Software Version 2A. Derived from internal Part Number 0302000G.Updated Section 4.3, Dilution Factor field comments.Removed all references to + Subset globally.Added WBC Threshold Full Histogram Group Fields.Added new Section 3.18, Collate the ToDo List.Updated General Information Group Fields from 29 groups to 24 groups.Added notes to Tables 4-3 and 4-15, respectively.Updated the following tables: 4-1; 4-3; 4-6; 4-8; 4-12; 4-13; 4-21; 4-30; 4-31; 4-33; 4-39;4-41; 4-46; 4-48; 4-52; 5-1; 5-2; 5-4. Removed NRBC note from Tables 4-24 and 4-46.Added Note: Parameter selection does not affect control transmissions. to Tables 4-43;4-44; 4-46; 4-48; and 4-50.Updated Control Information Group 34, removed referenceWBC Count Field.Updated Section 6.2, added SOH to table. Updated Section 8.1.

    Revision C, 9/03Software Version 2B. Derived from internal Part Number 0302000K. Updated the followingtables: 4-23; 4-24; 4-43; 4-4; 4-46; 4-48; 4-50.Added new section 3.19.

    Revision D, 9/04LH 700 Software Version 2B and LH 500 Software Version 2A. Derived from internal PartNumber 0302000N. Updated the following tables: 4.12; 4.20; 4.22; 4.25; 4.28; 4.30; 4.37;5.1.

    Revision E, 9/06

    LH 780 Software Version 1A, LH 750 Software Version 2D and LH 500 Software Version 2A.Derived from internal Part Number 0302000U.Section 3.3: Added paragraph, "Timeout settings at the host for all other responses."Updated the following tables: 5.4.

    Revision EA, 8/09LH 780 Software Version 1B1, LH 750 Software Version 2D1 and LH 500 Software Version2A. Derived from internal Part Number 0302000WA. Updated the following sections: 3.4;4.1, 4.2, 4.3, 5.3Updated the following tables: 4.3; 4.5; 4.6; 4.8; 4.9; 4.12; 4.13; 4.22; 4.24;4.26; 4.30; 4.32; 4.33; 4.36; 4.37; 4.44; 4.46; 4.48; 4.50; 4.54; 4.55; 5.1; 5.4.

    Revision EB, 10/10

    Software Version LH 780 1B1, Software Version LH 750 2D1, Software Version LH 500 2A

    Updates were made to the company corporate address.

    Note: Changes that are part of the most recent revision are indicated in text by a bar in themargin of the amended page.

    This document applies to the latest software listed and higher versions. When a subsequent software versionchanges the information in this document, a new issue will be released to the Beckman Coulter website. Forlabeling updates, go towww.beckmancoulter.com and download the most recent manual or system help foryour instrument.

    http://www.beckmancoulter.com/http://www.beckmancoulter.com/
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    REVISION STATUS

    Revision EC, 01/13LH 780 Software version 1B3, LH 750 Software version 2D3, LH 500 Software version 2A6

    Updates were made to:

    d HARDWARE INTERFACE

    d High Level Protocol

    d LIS COM PORT CONFIGURATION

    d Figure 8.12, Space for tower-type computers

    d Figure 8.13, Space for Small Form Factor (SFF)-type computers

    Note: Changes that are part of the most recent revision are indicated in text by a bar in themargin of the amended page.

    This document applies to the latest software listed and higher versions. When a subsequent software versionchanges the information in this document, a new issue will be released to the Beckman Coulter website. Forlabeling updates, go to www.beckmancoulter.com and download the most recent manual or system help foryour instrument.

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    CONTENTS

    REVISION STATUS, iii

    CONTENTS, v

    1 INTRODUCTION, 1-1

    1.1 PURPOSE, 1-1

    2 HARDWARE INTERFACE, 2-1

    2.1 HARDWARE HANDSHAKE, 2-2

    3 COMMUNICATION PARAMETERS, 3-1

    3.1 COMMUNICATION PARAMETERS, 3-1

    3.2 COMMUNICATION PROTOCOLS, 3-1Low Level Protocol, 3-1

    High Level Protocol, 3-1

    3.3 LIS COM PORT CONFIGURATION, 3-2

    3.4 TIME-OUT, 3-3

    3.5 BAUD RATE, 3-3

    3.6 DATA BITS, 3-3

    3.7 PARITY, 3-3

    3.8 STOP BITS, 3-4

    3.9 HANDSHAKE, 3-4

    3.10 BLOCK SIZE, 3-4

    3.11 GRAPHICS, 3-4

    3.12 COMPATIBILITY, 3-4

    3.13 SPOOLER, 3-4

    3.14 CLEARING THE SPOOLER, 3-4

    3.15 REPLACE NULL BY SP, 3-5

    3.16 DISABLING HOST COMMUNICATIONS, 3-5

    3.17 ENABLE VALIDATION CODES, 3-5

    3.18 Replace DLE E with DLE C, 3-5

    3.19 Collate the ToDo List, 3-5

    3.20 Replace V and v Flags with R (LH 500 Only), 3-5

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    CONTENTS

    3.21 Send LH 780 Specific Fields (LH 780 Only), 3-5

    4 LH SERIES WORKSTATION TO HOST COMMUNICATIONS, 4-1

    4.1 DATA-LINK, 4-1Full Software Handshake, 4-1Software Handshake Action, 4-1No Handshake, 4-2Data Block Structure, 4-2Group Definition Locator Table, 4-3

    4.2 1G.1 + RETICS FORMAT, 4-4Message Structure, 4-4

    Preamble, 4-4Group, 4-4Field Count, 4-4Fields, 4-5Postamble, 4-5General Information Group Fields, 4-6CBC Parameter Group Fields, 4-9DIFF Count Parameter Group Fields, 4-11DIFF Percent Parameter Group Fields, 4-12Comment Group Fields, 4-14Flag Group Fields, 4-14Demographics Group Fields, 4-18DF1 DataPlot Group Fields, 4-20DF2 DataPlot Group Fields, 4-20DIFF (VCS) Histogram Group Fields, 4-21

    RBC Histogram Group Fields, 4-21PLT Histogram Group Fields, 4-21RETIC Parameters Group Fields, 4-22DF5 LS DataPlot Group Fields, 4-24DF6 OP DataPlot Group Fields, 4-24RETIC Histogram Group, 4-25

    4.3 LH 750 WORKSTATION FORMAT, 4-25Message Structure, 4-25Group Definition, 4-28

    General Information Group Fields, 4-29CBC Parameters Group Fields, 4-34

    DIFF Count Parameters Group Fields, 4-35DIFF Percent Parameters Group Fields, 4-37Comment Group Fields, 4-38Definitive Flags Group Fields, 4-39Suspect Flags Group Fields, 4-40Conditional Flags Group Fields, 4-41Other Flags Group Fields, 4-43Demographics, 4-44RBC Histogram Group Fields, 4-48PLT Histogram Group Fields, 4-48

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    CONTENTS

    RETIC Parameters Group Fields, 4-49WBC Threshold Group Fields, 4-51WBC Threshold Full Histogram Group Fields, 4-51DIFF Latex Parameters Group Fields, 4-51

    RETIC Latex Parameters Group Fields, 4-53Control CBC Parameters Group Fields, 4-57Control DIFF Count Parameters Group Fields, 4-58Control DIFF Percent Parameters Group Fields, 4-60Control RETIC Parameters Group Fields, 4-61DIFF Graphics, 4-62Retic Graphics, 4-63Research Suspect, 4-63Research Population, 4-64

    4.4 HOST PARSING OF TRANSMITTED DATA, 4-71

    5 HOST TO LH SERIES WORKSTATION COMMUNICATIONS(HOST ToDo LIST), 5-1

    5.1 DATA-LINK, 5-1Protocol, 5-1Data Block Structure, 5-4

    5.2 PRESENTATION, 5-4Message Structure, 5-4

    DLE Responses From The Workstation And Effects After A ToDo ListDownload, 5-5

    5.3 MESSAGE DEFINITION, 5-7

    5.4 Delete Message, 5-16

    6 ASCII Tables, 6-1

    6.1 7-BIT ASCII CODES, 6-1

    6.2 VALID HOST COMMUNICATIONS ASCII CODES, 6-1

    7 CRC, 7-1

    7.1 CRC ALGORITHM, 7-1

    7.2 CRC EXAMPLE WRITTEN IN ASM86, 7-2

    7.3 CRC EXAMPLE WRITTEN IN C, 7-3

    8 GRAPHIC RECONSTRUCTION, 8-1

    8.1 RBC, WBC THRESHOLD, AND PLT HISTOGRAMS, 8-1

    8.2 LH 750 DATAPLOT FORMAT, 8-1Dataplots, 8-1

    Dataplot Data Header, 8-2

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    CONTENTS

    Dataplot Data Header Elements, 8-2Dataplot Data Block, 8-2Dataplot Data Block Header, 8-2Rendering Information Block, 8-3

    Rendering Information Block Header, 8-4Color Palette, 8-4Dithered Display Bitmap Library, 8-4Dataplot Header, 8-5

    8.3 COMPUTERS, 8-6

    BECKMAN COULTER, INC. CUSTOMER END USER LICENSE AGREEMENT, 1

    TRADEMARKS, 3

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    Illustrations3.1 Rear of instrument with DB 25-pin connector for tower-type computers, 3-23.2 Rear of instrument with DB 9-pin connector for SFF-type computers, 3-38.1 Plotting RBC, WBC Threshold and PLT Histograms, 8-1

    8.2 Dataplot Transmission, 8-28.3 Dataplot Data Header, 8-28.4 Dataplot Data Block Format, 8-28.5 Dataplot Data Block Header, 8-38.6 Rendering Information Block, 8-38.7 Rendering Information Block Header Format, 8-48.8 Palette Table Entry, 8-48.9 Bitmap Format, 8-58.10 Dataplot Header, 8-58.11 Dataplot Data Format, 8-68.12 Space for tower-type computers, 8-68.13 Space for Small Form Factor (SFF)-type computers, 8-7

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    Tables2.1 Signal and Flow Direction for the DB 25-pin connector, 2-12.2 Signal and Flow Direction for the DB 9-pin connector, 2-13.1 Communication Parameters Available by Format, 3-1

    3.2 COM Port Mapping, 3-23.3 Graphics Options, 3-44.1 Group Definition Locator, 4-34.2 Group Definition: 1G.1 + Retics, 4-64.3 General Information -- Group 1, 4-64.4 CBC Parameters -- Group 2, 4-94.5 Flag Data for CBC Parameter Group Fields, 4-104.6 DIFF Count Parameters -- Group 3, 4-114.7 Flag Data for Diff Count Parameter Group Fields, 4-124.8 DIFF Percent Parameters -- Group 4, 4-124.9 Flag Data for DIFF Percent Parameter Group Fields, 4-134.10 Comment -- Group 5, 4-144.11 Flag -- Group 6, 4-144.12 LH 750 Workstation/STKS 1G.1 + Retics Messages, 4-154.13 Demographics -- Group 7, 4-184.14 RBC Histogram -- Group 11, 4-214.15 PLT Histogram -- Group 12, 4-214.16 RETIC Parameters -- Group 13, 4-224.17 Flag Data for Retic Parameter Group Fields, 4-244.18 Transmission Tests and Test IDs by Test Type, 4-254.19 Transmitted Groups, 4-284.20 General Information -- Group 1, 4-294.21 Possible Values for Sample Status, 4-33

    4.22 CBC Parameters -- Group 2, 4-344.23 Flag Data for CBC Parameter Group Fields, 4-354.24 DIFF Count Parameters -- Group 3, 4-354.25 Flag Data for DIFF Count Parameter Group Fields, 4-364.26 DIFF Percent Parameters -- Group 4, 4-374.27 Flag Data for DIFF Percent Parameter Group Fields, 4-384.28 Comment -- Group 5, 4-384.29 Definitive Flags -- Group 6, 4-394.30 Suspect Flags -- Group 7, 4-404.31 Conditional Flags -- Group 8, 4-414.32 Other Flags -- Group 9, 4-434.33 Demographics -- Group 10, 4-44

    4.34 RBC Histogram -- Group 14, 4-484.35 PLT Histogram -- Group 15, 4-484.36 RETIC Parameters -- Group 16, 4-494.37 Flag Data for Retic Parameter Group Fields, 4-504.38 WBC Threshold Monitor Group 26, 4-514.39 DIFF Latex Parameters -- Group 31, 4-514.40 Flag Data for DIFF Latex Parameters Group Fields, 4-524.41 RETIC Latex Parameters -- Group 32, 4-534.42 Flag Data for RETIC Latex Parameters Group Fields, 4-544.43 Control Information -- Group 34, 4-54

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    4.44 Control CBC Parameters -- Group 35, 4-574.45 Flag Data for Control CBC Parameters Group Fields, 4-584.46 Control DIFF Count Parameters -- Group 36, 4-584.47 Flag Data for DIFF Count Parameter Group Fields, 4-59

    4.48 Control DIFF Percent Parameters -- Group 37, 4-604.49 Flag Data for Diff Percent Parameter Group Fields, 4-614.50 Control RETIC Parameters -- Group 38, 4-614.51 Flag Data for Control RETIC Parameter Group Fields, 4-624.52 DIFF DataPlot -- Group 50, 4-624.53 Retic DataPlot -- Group 51, 4-634.54 Research Suspect Flags -- Group 53 (LH 780 Only), 4-634.55 Research Population Group Fields -- Group 54 (LH 780 Only), 4-645.1 Message Definition Table, 5-75.2 Possible Values and Examples of Test Requests, 5-155.3 Possible Combinations of Sample Requests with Multiple Test Requests, 5-155.4 Patient ID Deletions, 5-17

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    1INTRODUCTION

    1.1 PURPOSEThis document is the specification for communication between the LH Series Workstationand a host computer. The functionality will remain backward compatible. This document isintended for use by software engineers needing to either maintain or modify the operation of

    the LH Series Workstation Host Communications.

    This document describes the transfer of data from the LH Series Workstation to an LIS/HIScomputer in the following formats:

    r STKS 1G.1 + Retics

    r LH 750 Workstation: host communication protocol which supports transmission ofCBC, DIFF and Reticulocytes parameters for samples and control runs as well ascollated sample runs. This format is an extension of the STKS 2A and 3A formats.

    The LH 750 STKS 1G.1 + Retics format is provided for use with existing STKS and MAXMinterfaces. Due to the LH Series Workstations new features and enhancements, STKS and

    MAXM instrument formats are not completely supported (see details in following sections).

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    INTRODUCTIONPURPOSE

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    2HARDWARE INTERFACE

    The system is equipped with a Host connector on the rear of the LH SeriesWorkstation thatlets the system interface with a host computer via a Standard EIA-type 25-pin connector anduses EIA Standard RS-232-C signals. The LH 500 is equipped with a 9 pin to 25 pin adapterallowing the system to interface with a host computer. The LH 500 and LH 700 Series tower

    computers must use the standard EIA-type 25 pin connector. The LH 700 Series Small FormFactor (SFF) must use a standard EIA-type 9-pin connector. This is a Data TerminalEquipment (DTE) configuration.

    The LH SeriesWorkstation/Host communications uses only the following signals:

    Table 2.1 Signal and Flow Direction for the DB 25-pin connector

    The LH SeriesWorkstation is the DTE, by RS-232 standards, which explains the RTS/DTRpin-outs. Whether the HOST is a DTE or DCE will determine its use of CTS/DSRorRTS/DTR. A DTE HOST requires the use of a NULL MODEM Cable.

    Pin number 5 CTS must be active for LH Series Workstation to send any transmissions. If thehost computer does not support the above hardware handshake lines, it will be necessary toconnect pin 4 to pin 5 and to connect pin 20 to pin 6.

    Table 2.2 Signal and Flow Direction for the DB 9-pin connector

    Pin No. Signal Name Flow Direction

    2 Transmit Data From LH Series Workstation

    3 Received Data To LH Series Workstation

    4 Ready To Send (RTS) From LH Series Workstation

    5 Clear To Send (CTS) To LH Series Workstation

    6 Data Set Ready (DSR) To LH Series Workstation

    7 Signal Ground Reference

    8 Carrier Detect Reference

    20 Data Terminal Ready (DTR) From LH Series Workstation

    22 Ring Indicator Reference

    Pin No. Signal Name Flow Direction

    2 Receive Data To LH Series Workstation

    3 Transmit Data From LH Series Workstation

    4 Data Terminal Ready (DTR) From LH Series Workstation

    5 Signal Ground Reference

    6 Data Set Ready (DSR) Reference

    7 Ready to Send (RTS) From LH Series Workstation

    8 Clear to Send (CTS) To LH Series Workstation

    9 Ring Indicator Reference

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    HARDWARE INTERFACEHARDWARE HANDSHAKE

    The LH Series Workstation is the DTE, by RS-232 Standards, which explains the RTS/DTRpin-outs. Whether the HOST is a DTE or DCE will determine its use of CTC/DSTorRTS/DTR. A DTE HOST requires the use of a NULL MODEM cable.

    Pin 8 CTS must be active for LH Series workstation to send any transmission. It is necessaryto connect pin 7 to pin 8. The 9-pin configuration cable uses the signals listed in Table 2.2,Signal and Flow Direction for the DB 9-pin connector. .

    2.1 HARDWARE HANDSHAKEDuring a transmission, the host logically raises CTS to allow the LH Series Workstation tosend data and logically lowers CTS to prevent the LH Series Workstation from sending data.

    Note: If CTS is lowered to hold off the LH Series Workstation host transmission, it must beraised again within the Workstation time-out period. If CTS is not raised within the time-outperiod, the LH Series Workstation transmission to the host will time-out and reset, ready forthe next transmission.

    Diagram of Hardware Handshake Mode

    LH Series Workstation (SENDER) Host (RECEIVER)

    send data ----->

    send data ----->

    - etc.-

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    3COMMUNICATION PARAMETERS

    3.1 COMMUNICATION PARAMETERSThe LH SeriesWorkstation allows the user to configure the following communicationparameters on the Host Computer Definition screen. These parameters includecommunication modes, as well as parameters enabling the transmission of graphic data.

    3.2 COMMUNICATION PROTOCOLSThe transmission specification consists of data-link (low level) and presentation (high level)protocols. Data-link protocol provides the means of transmitting data without any regard toactual information. Presentation protocol describes the actual information and its format.

    Low Level Protocol

    The Host Low Level Communication Protocol will be supported by all formats.

    High Level Protocol

    The High Level Communication Protocols currently supported are theSTKS 1G.1 + Retics and LH 750 Workstation. The High Level Communication Protocolis selected using the compatibility field.

    Note:To satisfy the host communication requirements, the receiver MUST parse for all of thedata.

    DONOTASSUMEFIXEDOFFSETS

    Table 3.1lists the communication parameters available by format.

    Table 3.1 Communication Parameters Available by Format

    Communication Parameter 1G.1 + Retics LH Series Workstation

    Time-out X X

    Baud Rate X X

    Data Bits X X

    Parity X X

    Stop Bits X X

    Handshake X X

    Block Size X X

    Graphics X X

    Compatibility X X

    COM Port X* X*

    X in 1G.1 + Retics column = Supported by the LH 750 STKS 1G.1 + Retics format.

    X in LH 750 Workstation column = Supported by the LH 750 Workstation format.

    * COM Port options are supported only by the LH 700 Series workstations.

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    COMMUNICATION PARAMETERSLIS COM PORT CONFIGURATION

    3.3 LIS COM PORT CONFIGURATIONIMPORTANT The current LIS cable that connects to the Equinox Host 1 port is different from the LIS cablethat connects to the COM Port 1 (pin layout is different). UI COM Port selection and physical communicationcable connection are based upon workstation type.

    For the LH 700 Series workstation, the COM Port can be configured to COM 1 or COM 6,depending on the workstation:

    r For the LH 700 Series tower-type computers, the default COM Port 6 must be usedwitha DB 25-pin connector. See Figure 8.12, Space for tower-type computersand Figure 3.1,Rear of instrument with DB 25-pin connector for tower-type computers.

    r For the LH 700 Series Small Form Factor computers (SFF), COM Port 1 must be usedwith a DB 9-pin connector. See Figure 8.13, Space for Small Form Factor (SFF)-typecomputersand Figure 3.2, Rear of instrument with DB 9-pin connector for SFF-typecomputers.

    Refer to the following Table 3.2, COM Port Mappingfor the correlation between ports and

    hardware configuration.

    Table 3.2 COM Port Mapping

    Figure 3.1 Rear of instrument with DB 25-pin connector for tower-type computers

    SW Port Configuration Workstation Labeling on Computer

    COM 6 Tower-type computer HOST 1 on Equinox cable

    COM 1 Small Form Factor computer COM 1

    DB 25-pin connector

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    COMMUNICATION PARAMETERSTIME-OUT

    Figure 3.2 Rear of instrument with DB 9-pin connector for SFF-type computers

    Note: The 9-pin serial connector on the computer base, designated as COM 1, is locatedbeside the video display connector.

    3.4 TIME-OUTThe time-out value determines the amount of time the LH SeriesWorkstation waits for aresponse from the host before retrying to send data to the host. The LH SeriesWorkstationcontinues to transmit at the time-out intervals until the host successfully receives the data.

    The time-out values are 30, 60, and 90 seconds. The default value is 90 seconds.

    Timeout settings at the host should be configured to optimize the flow of communications.While the LH Series Workstation responds to most transmissions very quickly, allowing alonger timeout for worst-case scenarios under heavy load conditions can help keep thecommunications synchronized, and therefore maximize overall communication throughput.We recommend allowing at least 90 seconds at the end of a host order transmission whilewaiting for the DLE response, and at least 30 seconds for all other responses.

    3.5 BAUD RATEThe following baud rates are supported: 2400, 4800, 9600, 19200 and 38400.

    Note:The minimum recommended baud rate is 2400 for non-graphic transmissions and 9600Baud for graphic transmissions.

    3.6 DATA BITSHost Communication can be configured for 7 or 8 bit mode.

    3.7 PARITYOdd, Even, and Off (None) parity modes are supported. Odd parity is the default value.

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    COMMUNICATION PARAMETERSSTOP BITS

    3.8 STOP BITSNone, 1, or 2 Stop Bit modes are supported. The default value is 2.

    3.9 HANDSHAKEThis option determines whether or not the LH SeriesWorkstation looks for a handshake fromthe host computer. See Heading 4.1, Data-Link, for additional information.

    3.10 BLOCK SIZEThe LH SeriesWorkstation/Host Communications support block sizes of 128 and 256 bytes.The default value is 256 bytes.

    3.11 GRAPHICSThe LH SeriesWorkstation allows DIFF and Retic graphic transmission if the compatibilityformat selected is the LH SeriesWorkstation format.

    Table 3.3lists the graphic items that can be enabled or disabled for the corresponding testbased on the current compatibility format and report profile selected.

    3.12 COMPATIBILITYThis option determines which transmission format is used by the LH SeriesWorkstation tosend results to an LIS/HIS computer. The Host High Level Communication Protocolssupported are: STKS 1G.1 + Retics and LH 750 Workstation.

    3.13 SPOOLERTransmissions to the Host are spooled before sending. When a transmission is sent to theHost, it will be kept on the Spooler and resent until the Host acknowledges that thetransmission was successfully received. If the Host is offline when Transmit To Host is

    enabled, all transmissions intended for the Host will be kept on the Spooler until the Hostcomes online or the Host Spooler is cleared.

    3.14 CLEARING THE SPOOLERSelect the Clear button in the LIS folder on the System Run Configuration screen to clear allspooled transmissions intended for the Host. It should be remembered that newtransmissions to the Host will still be subsequently spooled. See Section 3.15 to disable hostcommunications and hence the host spooler.

    Table 3.3 Graphics Options

    Test Graphics 1G.1 + Retics LH 750 Workstation

    CBC RBC Histogram X X

    PLT Histogram X X

    WBC Threshold N/A X

    DIFF DataPlot N/A X

    RETICS DataPlot N/A X

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    COMMUNICATION PARAMETERSREPLACE NULL BY SP

    3.15 REPLACE NULL BY SPThis is no longer an option because NULL characters are not included in the transmissionbuffer.

    3.16 DISABLING HOST COMMUNICATIONSSelect the Disable button in the LIS folder on the System Run Configuration screen to disableall auto transmissions from the LH SeriesWorkstation to the Host. This will not affect manualtransmissions or transmissions from the Host to the LH SeriesWorkstation.

    3.17 ENABLE VALIDATION CODESThis checkbox enables the codes used to identify the validation status of individual testrequests on a sample as detailed in Conditional Flags Group Fieldsunder AutoValidation inChapter 4. If Enable Validation Codes in System Setup - [Communications] is checked(default), the individual test group validation status will be transmitted. Applied only toLH 750 format.

    3.18 Replace DLE E with DLE CSelect this checkbox to replace DLE E with DLE C in the standard data set transmitted to yourinformation system.

    3.19 Collate the ToDo ListThe ToDo List generation is derived from task pre-assignment, by determining what sets ofdata are to be collated into a final report, test panels are pre-assigned either manually or byLIS download.

    3.20 Replace V and v Flags with R (LH 500 Only)If transmitting in the protocol STKS 1G.1 + Retics all transmissions of V and v flags will besent as R. If transmitting in the LH 750 Workstation protocol, transmissions of V and v flagswill be sent as R only if the Replace V and v flags with an R option is enabled, otherwise Vand v flags will be sent as V and v.

    3.21 Send LH 780 Specific Fields (LH 780 Only)Several new fields are added to the LH 780 transmissions in the LH 750 Workstationprotocol, if the option is enabled. These include new parameters, Research suspect messages,Research Population Data, and a Stat field.

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    COMMUNICATION PARAMETERSSend LH 780 Specific Fields (LH 780 Only)

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    4LH SERIES WORKSTATION TO HOST COMMUNICATIONS

    4.1 DATA-LINKA sample transmission to the Host consists of one or more framed data blocks. See Data BlockStructure below. All transmitted data bytes are ASCII characters. All numeric values are ASCIIrepresentations of hexadecimal numbers. Hence, for example, the number FF (hex) is

    represented by the two ASCII bytes "FF" (46H, 46H). The first two bytes of the transmissionare the number of blocks to be sent, in ASCII, followed by transmission of data blocks (seeformat below).

    The final block will be padded with ASCII spaces (20H) to fill a whole block.

    The LH Series Workstation supports full software handshake and no software handshakeprotocols.

    Full Software Handshake

    The LH Series Workstation sends the following control characters plus data and expects theindicated host response:

    Up to 255 blocks of data can be sent. The actual number of blocks sent is specified by thetwo-byte ASCII Block Count.

    Software Handshake Action

    If the host NAKs a data block, the block is retransmitted. It is up to the host to determine howmany times it retries receiving a NAKed block before aborting. Typically, the Host will NAK adatablock if the current block number is incorrect(e.g. out of sequence), the Hosts calculatedCRC does not match the transmitted blocks CRC or block framing is not recognized ascorrect.

    A SYN sent by the Host after a block has been transmitted by the LH Series Workstation forcesthe Workstation to retransmit all data starting with the first block. This does not include the

    LH Series Workstation(SENDER) HOST (RECEIVER)

    SYN (ready)------>

    for each block

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    initial SYN and block count. It is up to the Host to determine under what conditions totransmit a SYN.

    The LH Series Workstation continues to send the same sample transmission until the Host

    accepts it entirely (ACKs each block). To abort a transmission, the host must ACK each blockof the transmission and discard it locally.

    No Handshake

    If Handshake option is disabled, the protocol ignores all hardware and software hosthandshake responses. In addition, in the No Handshake mode, the LH SeriesWorkstationdoes not send a SYN prior to transmitting the data. CTS is always used to indicate that a Hostis MS and turned on.

    NOTE:The option to turn off handshaking is suppoprted for backward compatibility. This isnot recommended as it has been shown to cause problems at slower baud rates that mayresult in corrupted or last data (during high volume times).

    Data Block Structure

    Each data block is framed with a Start Of Text(STX = 02H) and an End Of Text(ETX =03H)

    control character.

    The next two bytes after STX have the Current Block Sequence Number(BLK NBR) in ASCIIstarting with the number 1(MS byte = 30H, LS byte = 31H, to give 01) for the first block. Theblock number of the last block must equal the Block Count sent at the beginning of thetransmission.

    Every data block will have either 128 or 256 bytes (whichever is chosen). If there isinsufficient data to fill a block, it will be padded with space characters (last blocktransmitted).

    Byte # # of bytes

    1 STX 1 byte

    2 BLK NBR MS CHAR 1 byte

    3 BLK NBR LS CHAR 1 byte

    4 DATA BYTES

    256 (128) C

    bytes RC

    259 (131)

    260 (132) CRC MSB MS CHAR 1 byte

    261 (133) CRC MSB LS CHAR 1 byte

    262 (134) CRC LSB MS CHAR 1 byte

    263 (135) CRC LSB LS CHAR 1 byte

    264 (136) ETX 1 byte

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    The CRC for the blocks data is calculated by the workstation and sent as the last four bytes ofthe block before the ETX in the ASCII format shown above. The Most Significant Byte (MSB)is generated as a Most Significant and Least Significant ASCII character (MS and LS CHAR).The same is true for the Least Significant Byte (LSB).

    The algorithm used to calculate the CRC for each block is a modified CCITT CRC16. CRC isonly calculated on the data bytes.

    Chapter 7details the CRC algorithm and includes application notes for implementing thealgorithm in both C and assembly language.

    Group Definition Locator Table

    Table 4.1lists the group names for each format.

    Table 4.1 Group Definition Locator

    Group Name

    Group

    Number

    1G.1 + Retics,

    Heading 4.2

    Group

    Number

    LH 750 ,

    Heading 4.3General Information 1 X 1 X

    CBC Parameters 2 X 2 X

    DIFF Count Parameters 3 X 3 X

    DIFF Percent Parameters 4 X 4 X

    Comment 5 X 5 X

    Definitive Flags 6 X

    Suspect Flags 7 X

    Conditional Flags 8 X

    Other Flags 9 X

    Flags 6 X

    Demographics 7 X 10 X

    DF1 DataPlot 8 X 11

    DF2 DataPlot 9 X 12

    DIFF (VCS) Histogram 10 X 13

    RBC Histogram 11 X 14 X

    PLT Histogram 12 X 15 X

    RETICS Parameters 13 X 16 X

    DF5 LS DataPlot 14 X 17

    DF6 OP DataPlot 15 X 18

    RETICS Histogram 16 X 19

    WBC Threshold 26 X

    WBC Threshold Full Histogram 27 X

    DIFF Latex Parameters 31 X

    RETIC Latex Parameters 32 X

    Control Information 34 X

    Control CBC Parameters 35 X

    Control DIFF Count Parameters 36 X

    Control DIFF Percent Parameters 37 X

    Control RETICS Parameters 38 X

    DIFF graphics 50 X

    Retic graphics 51 X

    Research Population Data ~ 52

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    4.2 1G.1 + RETICS FORMAT

    Message Structure

    The Presentation deals with the high level format of the message. The data bytes of thetransmission blocks, when collected together, exhibit the following high level format.

    Preamble

    The preamble marks the beginning of a message.

    Group

    The DC1 (11H) character marks the beginning of a Group. There is no relationship betweenthe order in which the groups are defined in the following sections and the order in whichthey appear after the preamble. Groups will never be omitted.

    Each Group contains a DC1 (11H) character, marking the beginning of the section, a twocharacter field count and a variable number of fields each ending with a CR (0DH) and an LF(0AH).

    Note: When there are no data for a Group, the Group will appear as follows:

    Field Count

    Each Group has a Field Count which identifies the number of fields contained within theGroup. Valid field counts can be from 0-255. The field count uses two characters to give theASCII representation of the hexadecimal value.

    Research Suspect Messages ~ 53

    X in 1G.1 + Retics column = Described under Heading 4.2, STKS 1G.1 + Retics Format.

    X in LH 750 Workstation column = Described under Heading 4.3, LH 750 Workstation Format.

    ~ LH 780 Only

    Table 4.1 Group Definition Locator (Continued)

    PREAMBLE GROUP x1 GROUP xn POSTAMBLE

    CR

    LF

    CR

    LF

    CR

    LF

    CR

    LF

    CR

    LF

    CR

    LF

    -

    -

    -

    -

    -

    -

    -

    -

    -

    -

    -

    -

    -

    -

    CR

    LF

    DC1

    FIELDCOUNT

    CR

    LF

    FIELD1

    CR

    LF

    FIELDN

    CR

    LF

    DC

    1

    0

    0

    CR

    LF

    MSCHAR

    LSCHAR

    Two-byte ASCII representation of Hexvalue between 00 and FF (0 to 255)

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    The field count will reflect the total number of fields transmitted within the Group. The fieldcount will always be the sum of CR/LF pairs within the Group minus one.

    Fields

    Each Group is made up of variable length fields which may or may not be padded withSP (20H) characters. A field may have a variable length tag preceding the data separated withone SP (20H) character. The data portion of a field will only contain ASCII characters in therange of 20H to 7EH inclusive. Chapter 6 tabulates the valid ASCII characters. Fields areseparated by a CR (0DH) and an LF (0AH) character.

    Note:A field's position within a Group must not be used to identify it. Fields within a Groupmay appear in any order or may be omitted. The CR/LF should be used to find a field and theTag used to identify it.

    The syntax for the Data Format of a field is:

    Postamble

    The postamble marks the end of the current message. There are 16 groups defined in thecurrent implementation of the STKS 1G.1 + Retics format. Future revisions may includeadditional groups. Any future additions will not disrupt the numbering of the groups aspresently defined.

    A - Alpha characters (a..z and A..Z and space) or (61H..7AH and 41H..5AH and 20H)N - Numeric characters (0..9 and + - .) or (30H..39H and 2BH 2DH 2EH)

    X - Printable characters (20H..7EH)

    Sep - Separator one or more SP characters

    SP Space (ASCII 20H). Please note that all field transmissions willinclude a SP between the tag and data format (this will be impliedin the following sections), as illustrated below:

    Tag Sep Data Format

    T I M E N N : N N : N N

    DC1

    CR

    LF

    CR

    LF

    -

    -

    -

    -

    -

    -

    -

    -

    -

    -

    -

    -

    -

    -

    CR

    LF

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    Group Definition

    Table 4.2lists the groups supported by the 1G.1 + Retics format.

    General Information Group Fields

    Table 4.3identifies the field names, test types, and applicable format of General InformationGroup transmissions.

    Table 4.2 Group Definition: 1G.1 + Retics

    GroupNumber Group Name 1G.1 +Retics

    1 General Information X

    2 CBC Parameters X

    3 DIFF Count Parameters X

    4 DIFF Percent Parameters X

    5 Comments X

    6 Flags X

    7 Demographics X

    8 DF1 DataPlot *

    9 DF2 DataPlot *

    10 DIFF (VCS) Histogram *

    11 RBC Histogram X

    12 PLT Histogram X

    13 Retic Parameter X

    14 DF5 DataPlot *

    15 DF6 DataPlot *

    16 Retic Histogram *

    X = Supported by the LH 750, STKS 1G.1 + Retics format.

    * = Not supported the LH 750 STKS 1G.1 + Retics format. The Group is transmitted with a field count of 00.

    Table 4.3 General Information -- Group 1

    Field Name Test Type LH 750 LH 780 LH 500

    Run Date C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    Run Time C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    ID #1 C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    ID #2 C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    Cassette/Position C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    Sequence Number C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    1E Cassette/Position C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    ID #1 Status C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    Cassette/Position Status C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

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    Run Date Field:

    Run Time Field:

    ID #1 Field:

    ID #2 Field:

    ToDo List Status C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    .

    Table 4.3 General Information -- Group 1

    Tag Data Format Data Length Comments

    D A T E N N / N N / N N 9 bytes Date output ismonth/day/year, regardlessof the system dateconfiguration.

    Tag Data Format Data Length Comments

    T I M E N N : N N : N N 12 bytes Time output is on 24 hours,regardless of the systemtime configuration. Timeouput ishours:minutes:seconds..

    Tag Data Format Data Length Comments

    I D 1 X X X X X X X X 16 bytes SAMPLE ID

    Tag Data Format Data Length Comments

    I D 2 X X X X X X X X 16 characters Concatenation of Patientlast and first nameseparated by a commaand a space. Only thefirst 16 characters of theconcatenated string aretransmitted.Example: Doe, John.

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    Cassette/Position Field:

    Sequence Number:

    1E Cassette/Position Field:

    ID #1 Status Field:

    Cassette/Position Status Field:

    Tag Data Format Data Length Comments

    C A S S / P O S X X X X / X X 8 bytes CASS/POS output is

    cassettenumber/cassetteposition.SECONDA - Manualsampling mode.

    Tag Data Format Data Length Comments

    S E Q U E N C E X X X X X X 7 characters Sequence Number

    Tag Data Format Data Length Comments

    1 E C / P A X X X X X X 8 bytes Date Values:S - Manual samplingmode - Automatic samplingmode.

    Tag Data Format Data Length Comments

    I D 1 S T A T U S A 2 bytes Data Values: P - positive ID. E - edited ID. - not edited and not positive ID. N - Auto-numbered.Precedence order is E, N, P and .

    Tag Data Format Data Length Comments

    C / P S T A T U S A 2 bytes Data Values: P - positive ID. E - edited ID. - not edited and not positive ID.Precedence order is: E, P and .

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    ToDo List Status:

    Note: On Japanese and Chinese systems, if the Enable DBCS Transmission option isenabled, the characters may be in combination of single and double byte characters, using the

    DBCS format.

    CBC Parameter Group Fields

    Table 4.4identifies the field names, test types, and applicable format of CBC ParameterGroup transmissions

    Tag Data Format Data Length Comments

    W L S T A T U S A A A A A A A A 9 bytes See note below.*

    *Data Values:

    GOOD = O.K. (Complete)

    PART ASP = Partial aspiration

    NO READ = Bar code not read

    NO MATCH = Match not found in ToDo list

    (20H) = Primary ID is cassette position and sample ID does not match preassigned ID

    Table 4.4 CBC Parameters -- Group 2

    Field Name Test Type 1G.1 + Retics

    WBC C, CD, CDR, CR, Edited X

    RBC C, CD, CDR, CR, R, Edited X

    HGB C, CD, CDR, CR, Edited XHCT C, CD, CDR, CR, Edited X

    MCV C, CD, CDR, CR, Edited X

    MCH C, CD, CDR, CR, Edited X

    MCHC C, CD, CDR, CR, Edited X

    RDW C, CD, CDR, CR, Edited X

    PLT C, CD, CDR, CR, Edited X

    PCT C, CD, CDR, CR, Edited X

    MPV C, CD, CDR, CR, Edited X

    PDW C, CD, CDR, CR, Edited X

    X = Supported by the LH 750, STKS 1G.1 + Retics format.

    For Retic only samples, only the RBC parameter is applicable. If the RBC parameter value is notavailable, then it will not be transmitted and this group will have a field count of 00.

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    Each field in the CBC Parameters Group will have the following format:

    Result Format:**

    The parameter result will be transmitted in the Reporting Units with the extra digit criteriaselected on the LH SeriesWorkstation.

    Numeric Data: **

    If the numeric data of each format (for example, xx.xx) does not contain a decimal number,then it contains one of the following: Flag Data: **

    The flag data characters for the CBC Parameter Group Fields are as follows in Table 4.5.Table 4.5lists all the possible flags generated on the LH Series Workstation and how theytranslate to the STKS 1G.1 + Retics format.

    Tag Data Format Data Length Comments

    A A A A X X X X X X A A A See Data

    Format column.

    See Below.**

    Result Comments

    . . . . . Incomplete computation

    + + + + + Parameter overrange

    - - - - - Parameter voteout

    Table 4.5 Flag Data for CBC Parameter Group Fields

    Comments

    STKS 1G.1 FormatsTranslation

    (3 characters)

    Review parameter. R

    Exceeds reference interval high limit H

    Exceeds reference interval low limit L

    Exceeds action high limit H

    Exceeds action low limit L

    Exceeds critical high limit H

    Exceeds critical low limit L

    Operator edited result E

    Recalculated result from edited result e

    MCV threshold failure * R

    Delta check failed

    Partial aspiration/blood detector disabled R

    Result exceeds linearity

    Single aperture voteout R

    6 char numericdata max.

    3 char flagdata max.

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    DIFF Count Parameter Group Fields

    Table 4.6identifies the field names, test types, and applicable format of DIFF CountParameter Group transmissions.

    This group is applicable to CBC/DIFF and CBC/DIFF/Retic sample runs. For all othersample runs, this group will have a field count of 00.

    Each field in the DIFF Count Parameters Group will have the following format:

    Result Format: **

    The parameter result will be transmitted in the Reporting Units with the extra digit criteriaselected on the LH Series Workstation.

    Numeric Data: **

    If the numeric data of each format (for example, xx.xx) does not contain a decimal number,then it contains one of the following:

    Result calculated from Single ApertureVoteout

    R

    * = Not supported by the STKS 1G.1 + Retics format.

    Table 4.6 DIFF Count Parameters -- Group 3

    Field Name Test Type LH 750 LH 780 LH 500

    LY# CD, CDR, Edited CD, CDR, Edited CD, CDR, Edited CD, Edited

    MO# CD, CDR, Edited CD, CDR, Edited CD, CDR, Edited CD, Edited

    NE# CD, CDR, Edited CD, CDR, Edited CD, CDR, Edited CD, Edited

    EO# CD, CDR, Edited CD, CDR, Edited CD, CDR, Edited CD, Edited

    BA# CD, CDR, Edited CD, CDR, Edited CD, CDR, Edited CD, Edited

    Tag Data Format Data Length Comments

    A A A A X X X X X X A A A See DataFormatcolumn.

    See Below.**

    Result Comments

    . . . . . Incomplete computation

    : : : : : Flow cell clogged (Full clog, Partial clog 1 or Partial clog 2)

    Table 4.5 Flag Data for CBC Parameter Group Fields

    6 char numericdata max.

    3 char flagdata max.

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    Flag Data: **

    The flag data characters for the DIFF Count Parameter Group fields are as follows inTable 4.7:

    DIFF Percent Parameter Group Fields

    Table 4.8identifies the field names, test types, and applicable format of DIFF PercentParameter Group transmissions.

    This group is applicable to CBC/DIFF and CBC/DIFF/Retics sample runs. For all othersample runs, this group will have a field count of 00.

    ????? Invalid data: could not calculate DIFF#.

    + + + + + WBC parameter overrange or sum of DIFF# parameters is greater than 500.0 x106cells/L (whole blood) or sum of DIFF# parameters is greater than 600.0 x

    106cells/L (predilute).

    Table 4.7 Flag Data for Diff Count Parameter Group Fields

    Comments

    STKS 1G.1 FormatsTranslation

    (3 characters)

    Review parameter: WBC threshold failure

    or WBC exceeds linearity or sum ofDIFF% results exceeds 100.

    R

    Exceeds reference interval high limit H

    Exceeds reference interval low limit L

    Exceeds action high limit H

    Exceeds action low limit L

    Exceeds critical high limit H

    Exceeds critical low limit L

    Operator edited result E

    Recalculated result from edited result e

    Parameter value exceeds Delta checklimits

    Partial aspiration/blood detector disabled R

    Result exceeds linearity

    Table 4.8 DIFF Percent Parameters -- Group 4

    Field Name Test Type LH 750 LH 780 LH 500

    LY% CD, CDR, Edited CD, CDR, Edited CD, CDR, Edited CD, EditedMO% CD, CDR, Edited CD, CDR, Edited CD, CDR, Edited CD, Edited

    NE% CD, CDR, Edited CD, CDR, Edited CD, CDR, Edited CD, Edited

    EO% CD, CDR, Edited CD, CDR, Edited CD, CDR, Edited CD, Edited

    BA% CD, CDR, Edited CD, CDR, Edited CD, CDR, Edited CD, Edited

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    Each field in the DIFF Percent Parameters Group will have the following format:

    Result Format:**

    The parameter result will be transmitted in the Reporting Units with the extra digit criteriaselected on the LH Series Workstation.

    Numeric Data: **

    If the numeric data of each format (for example, xx.xx) does not contain a decimal number,then it contains one of the following:

    Flag Data: **

    The flag data characters for the DIFF Percent Parameter Group fields will be as follows inTable 4.9:

    Tag Data Format Data Length Comments

    A A A A X X X X X X A A A See Data

    Formatcolumn.

    See Below.**

    Result Comments

    . . . . . Incomplete computation

    : : : : : Flow cell clogged (Full clog, Partial clog 1 or Partial clog 2)

    + + + + + Sum of DIFF% parameter results is not equal to 100% +0.1, or DIFF# parameters is

    greater than 600.0 x 106cells/L (predilute).

    Table 4.9 Flag Data for DIFF Percent Parameter Group Fields

    Comments

    STKS 1G.1 FormatsTranslation

    (3 characters)

    Review parameter R

    Exceeds reference interval high limit H

    Exceeds reference interval low limit L

    Exceeds action high limit H

    Exceeds action low limit L

    Exceeds critical high limit H

    Exceeds critical low limit L

    Operator edited result E

    Recalculated result from edited result e

    Delta check failed

    6 char numericdata max.

    3 char flagdata max.

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    Comment Group Fields

    Table 4.10identifies the field names, test types, and applicable format of Comment Grouptransmissions.

    .

    The Comment Group can have a variable number of fields which consist of a variable numberof characters. Each Comment field is made up of an ASCII string identifying the appropriateflag.

    Note: On Japanese and Chinese systems, if the Enable DBCS Transmission option isenabled, the characters may be in combination of single and double byte characters, using theDBCS format.

    Flag Group Fields

    Table 4.11identifies the field names, test types, and applicable format of Flag Grouptransmissions.

    Note: This group will always be transmitted and will only be sent once per sampletransmission.

    The Flag Group can have a variable number of fields that consist of a variable number ofbytes. There are no Tags associated with a Flag field. Each Flag field is made up of an ASCIIstring identifying the appropriate flag.

    Partial aspiration/blood detector disabled R

    Result exceeds linearity

    Table 4.10 Comment -- Group 5

    Field Name Test Type 1G.1 + Retics LH 750 LH 780 LH 500

    Comment C, CD,CDR, CR, R

    C, CD,CDR, CR, R

    C, CD,CDR, CR, R

    C, CD,CDR, CR, R

    C, CD, R

    Data Format Data Length Comments

    X X X X X X X X X X X X X X X 64 charactersmaximum

    Table 4.11 Flag -- Group 6

    Field Name Test Type LH 750 LH 780 LH 500

    Variable C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    Data Format Data Length Comments

    X X X X X X X X X X X X X X X Variable

    Table 4.9 Flag Data for DIFF Percent Parameter Group Fields

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    Table 4.12lists all the possible flags generated on the LH Series Workstation and how theytranslate to the STKS 1G.1 + Retics format. For example, a Low Event # suspect flagmessage in LH 750 format is a Review message and an Abnormal WBC Pop message in STKS1G.1 + Retics format.

    Table 4.12 LH 750 Workstation/STKS 1G.1 + Retics Messages

    LH Series Workstation Message STKS 1G.1 + Retics

    Suspect Flags

    Low Event # Review Slide Abnormal WBC Pop

    Verify Diff Review Slide Abnormal WBC Pop

    Ne Blasts Blasts Abnormal WBC Pop

    Ly Blasts Blasts Abnormal WBC Pop

    Mo Blasts Blasts Abnormal WBC Pop

    Imm. NE 1 Imm Grans/Bands 1 Abnormal WBC Pop

    Imm. NE 2 Imm Grans/Bands 2 Abnormal WBC Pop

    Variant LY Variant Lymphs Abnormal WBC Pop

    NRBC NRBCs Abnormal RBC Pop

    WBC Interference (N/A) (N/A)

    RBC Interference (N/A) (N/A)

    Dimorphic Reds Dimorphic RBC Pop Abnormal RBC Pop

    Red Cell Agglutination RBC Agglutination Abnormal RBC Pop

    Verify Retic (N/A) Verify Retic

    Abnormal Retic Pattern (N/A) (N/A)

    Platelet Clumps Platelet Clumps Abnormal PLT Pop

    Giant Platelets Giant Platelets Abnormal PLT Pop

    WBC Exceeds Linearity (N/A) (N/A)

    Definitive Flags

    Leukopenia Leukopenia Abnormal WBC Pop

    Leukocytosis Leukocytosis Abnormal WBC Pop

    Neutropenia % Neutropenia % Abnormal WBC Pop

    Neutropenia # Neutropenia # Abnormal WBC Pop

    Neutrophilia % Neutrophilia % Abnormal WBC Pop

    Neutrophilia # Neutrophilia # Abnormal WBC Pop

    Lymphopenia % Lymphopenia % Abnormal WBC Pop

    Lymphopenia # Lymphopenia # Abnormal WBC Pop

    Lymphocytosis % Lymphocytosis % Abnormal WBC Pop

    Lymphocytosis # Lymphocytosis # Abnormal WBC Pop

    Monocytosis % Monocytosis % Abnormal WBC Pop

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    LH SERIES WORKSTATION TO HOST COMMUNICATIONS1G.1 + RETICS FORMAT

    Monocytosis # Monocytosis # Abnormal WBC Pop

    Eosinophilia % Eosinophilia % Abnormal WBC Pop

    Eosinophilia # Eosinophilia # Abnormal WBC Pop

    Basophilia % Basophilia % Abnormal WBC Pop

    Basophilia # Basophilia # Abnormal WBC Pop

    Anemia Anemia Abnormal RBC Pop

    Anisocytosis Anisocytosis Abnormal RBC Pop

    1+ Anisocytosis 1+ Anisocytosis Abnormal RBC Pop

    2+ Anisocytosis 2+ Anisocytosis Abnormal RBC Pop

    3+ Anisocytosis 3+ Anisocytosis Abnormal RBC Pop

    Microcytosis Microcytosis Abnormal RBC Pop

    1+ Microcytosis 1+ Microcytosis Abnormal RBC Pop

    2+ Microcytosis 2+ Microcytosis Abnormal RBC Pop

    3+ Microcytosis 3+ Microcytosis Abnormal RBC Pop

    Macrocytosis Macrocytosis Abnormal RBC Pop

    1+ Macrocytosis 1+ Macrocytosis Abnormal RBC Pop

    2+ Macrocytosis 2+ Macrocytosis Abnormal RBC Pop

    3+ Macrocytosis 3+ Macrocytosis Abnormal RBC Pop

    Hypochromia Hypochromia Abnormal RBC Pop

    1+ Hypochromia 1+ Hypochromia Abnormal RBC Pop

    2+ Hypochromia 2+ Hypochromia Abnormal RBC Pop

    3+ Hypochromia 3+ Hypochromia Abnormal RBC Pop

    (N/A) 1+ Poikilocytosis Abnormal RBC Pop

    (N/A) 2+ Poikilocytosis Abnormal RBC Pop

    (N/A) 3+ Poikilocytosis Abnormal RBC Pop

    Erythrocytosis Erythrocytosis Abnormal RBC Pop

    Pancytopenia Pancytopenia Abnormal WBC Pop andAbnormal RBC Pop and

    Abnormal PLT PopReticulocytosis (N/A) (N/A)

    Thrombocytopenia Thrombocytopenia Abnormal PLT Pop

    Thrombocytosis Thrombocytosis Abnormal PLT Pop

    Small Platelets Small Platelets Abnormal PLT Pop

    Large Platelets Large Platelets Abnormal PLT Pop

    H&H Check Failed (N/A) (N/A)

    Table 4.12 LH 750 Workstation/STKS 1G.1 + Retics Messages (Continued)

    LH Series Workstation Message STKS 1G.1 + Retics

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    Parameter Flags

    . . . . . . . . . . Abnormal WBC Pop and/orAbnormal RBC Pop and/orAbnormal PLT Pop and/orVerify Retic

    + + + + + + + + + + Abnormal WBC Pop and/orAbnormal RBC Pop and/orAbnormal PLT Pop

    - - - - - - - - - - Abnormal WBC Pop and/orAbnormal RBC Pop and/orAbnormal PLT Pop and/orVerify Retic

    ????? ????? Abnormal WBC Pop and/orAbnormal RBC Pop and/orAbnormal PLT Pop and/orVerify Retic

    : : : : : : : : : : Abnormal WBC Pop and/orAbnormal RBC Pop and/orAbnormal PLT Pop and/orVerify Retic

    R R (N/A)

    H H (N/A)

    L L (N/A)

    E E Edited Data

    e e Edited Data

    D (N/A) (N/A)

    + +++++ Abnormal WBC Pop and/orAbnormal RBC Pop and/orAbnormal PLT Pop and/orVerify Retic

    Other Flags

    Edited Data Edited Data (N/A)

    Preliminary Report Preliminary Report (N/A)Additional Test Report (N/A) (N/A)

    Incomplete Collation Collate Failed (N/A)

    SYSTEM ALARM (N/A) (N/A)

    Completed Report (N/A) (N/A)

    Blood Detector Disabled (N/A) (N/A)

    Sample validated (N/A) (N/A)

    Table 4.12 LH 750 Workstation/STKS 1G.1 + Retics Messages (Continued)

    LH Series Workstation Message STKS 1G.1 + Retics

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    Note: These flags will be produced only for the research parameters that are transmitted.

    Demographics Group Fields

    Table 4.13identifies the field names, test types, and applicable format of DemographicsGroup transmissions.

    Note:This group will be transmitted only once per sample transmission.

    Sample not validated (N/A) (N/A)

    AutoValidated (N/A) (N/A)

    Table 4.12 LH 750 Workstation/STKS 1G.1 + Retics Messages (Continued)

    LH Series Workstation Message STKS 1G.1 + Retics

    STKS Messages

    PCT IS FOR RESEARCH ONLY, NOT FOR DIAGNOSTIC PROCEDURES

    PDW IS FOR RESEARCH ONLY, NOT FOR DIAGNOSTIC PROCEDURES

    Table 4.13 Demographics -- Group 7

    Field Name Test Type LH 750 LH 780 LH 500

    Date of Birth C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    User Field #1 C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    User Field #2 C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    User Field #3 C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    Sex C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    Location C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    Physician C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    User Date C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    User Time C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

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    Date of Birth Field:

    User Field #1:

    User Field #2:

    User Field #3:

    Sex Field:

    Location Field:

    Tag Data Format Data Length Comments

    B I R T H N N / N N / N N N N 11 bytes Date of birth output

    is month/day/year,regardless of thesystem dateconfiguration.The Date Of BirthYear is Always 4Characters.

    Tag Data Format Data Length Comments

    U F 1 X X X X X 17 characters

    Tag Data Format Data Length Comments

    U F 2 X X X X X 17 characters

    Tag Data Format Data Length CommentsU F 3 X X X X X 17 characters

    Tag Data Format Data Length Comments

    S E X A 2 bytes Data Value:M MaleF Female (20H) Unknown

    Tag Data Format Data Length Comments

    L O C A T I O N X X X X X 17 characters

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    Physician Field:

    User Date Field:

    User Time Field:

    Note: On Japanese and Chinese systems, if the Enable DBCS Transmission option isenabled, the characters may be in combination of single and double byte characters, using theDBCS format.

    DF1 DataPlot Group Fields

    The 1G.1 + Retics format does not support transmission of this group. This group will betransmitted with field count of 00.

    DF2 DataPlot Group Fields

    The 1G.1 + Retics format does not support transmission of this group. This group will betransmitted with field count of 00.

    Tag Data Format Data Length Comments

    P H Y S I C I A N X X X X X X 22 characters Concatenation of

    Physician last andfirst name separatedby a comma and aspace. Only the first22 characters of theconcatenated stringare transmitted.Example: Smith,Peter.

    Tag Data Format Data Length CommentsU D A T E N N / N N / N N 9 bytes Sample Drawn Date.

    Output format ismonth/day/year,regardless of thesystem dateconfiguration.

    Tag Data Format Data Length Comments

    U T I M E N N : N N : N N 9 bytes Sample Drawn Time.Output is on 24hours, regardless ofthe system timeconfiguration.Time output ishours:minutes:seconds.

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    DIFF (VCS) Histogram Group Fields

    The 1G.1 + Retics format does not support transmission of this group. This group will betransmitted with field count of 00.

    RBC Histogram Group FieldsTable 4.14identifies the field names, test types, and applicable format of RBC HistogramGroup Field transmissions.

    If RBC histogram graphic transmission is disabled, this group is transmitted with a field of 00.

    RBC Histogram Field:

    PLT Histogram Group Fields

    Table 4.15identifies the field names, test types, and applicable format of PLT HistogramGroup Field transmissions.

    If PLT histogram graphic transmission is disabled, this group is transmitted with a field of 00.

    Note: When the PLT histogram graphic transmission is enabled the data in the PLTF field isalways sent. Ignore the data sent in the PLTF field if an R flag is present for the PLT parameter

    sent in the CBC Parameter Group.

    PLT Histogram Field:

    Table 4.14 RBC Histogram -- Group 11

    Field Name Test Type LH 750 LH 780 LH 500

    RBC Histogram C, CD, CDR, CR C, CD, CDR, CR C, CD, CDR, CR C, CD

    Tag Data Format Data Length Comments

    R B C H H H H H H H 512 bytes See Chapter 8 forgraphicreconstruction.

    Table 4.15 PLT Histogram -- Group 12

    Field Name Test Type LH 750 LH 780 LH 500

    PLT Histogram C, CD, CDR, CR C, CD, CDR, CR C, CD, CDR, CR C, CD

    PLT Fit Histogram C, CD, CDR, CR C, CD, CDR, CR C, CD, CDR, CR C, CD

    Tag Data Format Data Length Comments

    P L T H H H H H H H 256 bytes See Chapter 8forgraphicreconstruction.

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    PLT Fit Histogram Field:

    RETIC Parameters Group Fields

    Table 4.16identifies the field names, test types, and applicable format of RETIC ParametersGroup Field transmissions.

    Retic Run Date Field:

    Tag Data Format Data Length Comments

    P L T F H H H H H H 512 bytes See Chapter 8for

    graphicreconstruction.

    Table 4.16 RETIC Parameters -- Group 13

    Field Name Test Type LH 750 LH 780 LH 500

    Retic Run Date CDR, CR, R CDR, CR, R CDR, CR, R R

    Retic Run Time CDR, CR, R CDR, CR, R CDR, CR, R RRetic ID CDR, CR, R CDR, CR, R CDR, CR, R R

    Retic Cassette Position CDR, CR, R CDR, CR, R CDR, CR, R R

    RET% CDR, CR, R,Edited

    CDR, CR, R,Edited

    CDR, CR, R,Edited

    R, Edited

    RET# CDR, CR, R,Edited

    CDR, CR, R,Edited

    CDR, CR, R,Edited

    R, Edited

    MRV CDR, CR, R,Edited

    CDR, CR, R,Edited

    CDR, CR, R,Edited

    R, Edited

    MI (now called IRF) CDR, CR, R,Edited

    CDR, CR, R,Edited

    CDR, CR, R,Edited

    R, Edited

    Tag Data Format Data Length Comments

    R E T D A T E N N / N N / N N 9 bytes Retic Run Date. Output ismonth/day/year, regardlessof the system timeconfiguration.Note: In the case of aCBC/Retic orCBC/Diff/Retic sample

    run, the Date and Time ofthe General Informationgroup are duplicated in theRETDATE and RETTIMEfields.

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    Retic Run Time Field:

    Retic ID Field:

    **Note: In the case of a CBC/Retic or CBC/DIFF/Retic sample run, the Sample IDtransmitted in the ID1 field is duplicated in this field.

    Retic Cassette/Position Field:

    Retic Parameter Results Field:

    Result Format:**

    1. The parameter result will be transmitted in the Reporting Units with the extra digitcriteria selected on the LH Series Workstation.

    Tag Data Format Data Length Comments

    R E T T I M E N N : N N : N N 9 bytes Retic Run Time. Output is

    on 24 hours, regardless ofthe system timeconfiguration. Time outputis hours:minutes:seconds.Note: In the case of aCBC/Retic orCBC/Diff/Retic samplerun, the Date and Time ofthe General Informationgroup are duplicated in theRETDATE and RETTIMEfields.

    Tag Data Format Data Length Comments

    R I D X X X X X 17 bytes See Note**

    Tag Data Format Data Length Comments

    R C A S S / P O S X X X X / X X 8 bytes This field only appliesto the 1G.1+ Reticshost transmissionprotocol.

    Tag Data Format Data Length Comments

    A A A A X X X X X X A A A See DataFormat

    column.

    See Below.**

    6 char numericdata max.

    3 char flagdata max.

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    Numeric Data: **

    If the numeric data of each format (for example, xx.xx) does not contain a decimal number,then it contains one of the following:

    Flag Data: **

    The flag data characters for the Retic Parameter Group fields will be as follows in Table 4.17:

    If a Retic parameter is disabled, results for that parameter will NOT be transmitted. The IRFparameter result is transmitted with the label MI for backward compatibility reasons.

    DF5 LS DataPlot Group Fields

    1G.1 + Retics format does not support transmission of this group. This group will betransmitted with field count of 00.

    DF6 OP DataPlot Group Fields

    1G.1 + Retics format does not support transmission of this group. This group will betransmitted with field count of 00.

    Result Comments

    . . . . . Incomplete computation

    : : : : : Flow cell clogged (Full clog, Partial clog 1 or Partial clog 2)

    ????? Invalid data: could not calculate RET#.

    + + + + + Retic parameter result overrange

    RET# > 999.9 x 106cells/LRET% > 100%

    Table 4.17 Flag Data for Retic Parameter Group Fields

    LH 750 Workstation Comments STKS 1G.1 + Retics

    Review parameter R

    Exceeds reference interval high limit H

    Exceeds reference interval low limit L

    Exceeds action high limit H

    Exceeds action low limit L

    Exceeds critical high limit HExceeds critical low limit L

    Operator edited result E

    Recalculated result from edited result e

    Delta check failed

    Partial aspiration/blood detector disabled R

    Result exceeds linearity

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    RETIC Histogram Group

    1G.1 + Retics format does not support transmission of this group. This group will betransmitted with field count of 00.

    4.3 LH 750 WORKSTATION FORMAT

    Message Structure

    The Presentation deals with the high level format of the message. The data bytes of thetransmission blocks, when collected together, exhibit the following high level format.

    Preamble:The preamble marks the beginning of a message.

    Transmission Identification:

    The ASCII character "S" marks the beginning of the transmission.

    TRANSMISSION TEST COUNT defines the number of tests that will be transmitted in thisparticular transmission. Not all Test Types are sent in all transmissions.

    Each Transmission Test is identified by the order in which it appears after the preamble. Thetransmission test will never be transmitted out of order.

    The Transmission Tests are up to 32 characters long. Table 4.18lists the availableTransmission Tests and Test IDs by Test Type.

    PREAMBLE TRANSMISSION IDENTIFICATION TEST a IDENTIFICATION GROUP x1

    GROUP xn

    TESTzIDENTIFICATION GROUP x1

    GROUP xn POSTAMBLE

    CR

    LF

    CR

    LF

    CR

    LF

    CR

    LF

    CR

    LF

    CR

    LF

    -

    -

    -

    -

    -

    -

    -

    -

    -

    - -

    -

    -

    -

    CR

    LF

    S TRANSMISSIONTEST

    COUNT

    CR

    LF

    TRANSMISSIONTEST

    1

    CR

    LF O O O

    TRANSMISSIONTEST

    N

    CR

    LF

    Table 4.18 Transmission Tests and Test IDs by Test Type

    Test Type Transmission Tests Test IDs

    C CBC CBC

    CD CBC, DIFF CBC, DIFF

    R RETIC RETIC

    CR CBC, RETIC CBC, RETIC

    CDR CBC, DIFF, RETIC CBC, DIFF, RETIC

    C & CD Control 5CC CBC, DIFF

    R Control RETICC RETIC

    CR Control RETICC CBC, RETIC

    CDRControl RETICC CBC, DIFF, RETIC

    CD Latex DLATEX CBC, DIFF

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    Currently only manually entered data is transmitted.

    Test Identification:

    The ASCII character "T" marks the beginning of a test identification.

    TEST ID, see Table 4.18.

    GROUP COUNT defines the number of groups of data in this particular TEST Identification.

    Group:

    The ASCII character "G" marks the beginning of a group. A group follows the testidentification fields.

    GROUP NUMBER defines the group number. See Group Definition.

    FIELD COUNT defines the number of fields in a particular group. See Field Count.

    FIELD defines a field within a group. See Fields.

    Note: When there is no data for a Group, the Group will not be sent.

    Test Type Count:The transmission identification section has a Test Type Count that identifies the number oftests contained within the transmission. Valid test type count can be from 0-255. The test typecount uses two characters to give the ASCII representation of the hexadecimal value.

    Group Count:Each test identification has a Group Count that identifies the number of groups containedwithin the test. Valid group counts can be from 0-255. The group count uses two characters

    to give the ASCII representation of the hexadecimal value.

    Group Number:Each group identifier has a Group Number that identifies the group number contained within

    CDR Latex LATEXC CBC, DIFF, RETIC

    Retic Latex RLATEX RETIC

    T TESTID

    CR

    LF

    GROUPCOUNT

    CR

    LF

    G GROUPNUMBER

    CR

    LF

    FIELDCOUNT

    CR

    LF

    FIELD1

    CR

    LF

    FIELDN

    CR

    LF

    MSCHAR

    LSCHAR

    Two-byte ASCII representation of Hexvalue between 00 and FF (0 to 255)

    MSCHAR

    LSCHAR

    Two-byte ASCII representation of Hexvalue between 00 and FF (0 to 255)

    Table 4.18 Transmission Tests and Test IDs by Test Type

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    the group identifier. Valid group number can be from 0-255. The group number uses two

    characters to give the ASCII representation of the hexadecimal value.

    Field Count:Each Group has a Field Count that identifies the number of fields contained within theGroup. Valid field counts can be from 0-255. The field count uses two characters to give the

    ASCII representation of the hexadecimal value.

    Fields:Each Group is made up of variable length fields which may or may not be padded with SP

    (20H), or HT (09H) characters. A field may have a variable length tag preceding the data,separated by one SP (20H) character. Please note that all field transmissions will include a SPbetween the tag and data format (this will be implied in the following sections), as illustrated

    below:

    The data portion of a field will only contain ASCII characters in the range of 20H to 7EHinclusive. Heading 6.2, VALID HOST COMMUNICATIONS ASCII CODESidentifies the validASCII characters. Fields are separated with a CR (0DH) and an LF (0AH) character.

    Note:A field's position within a Group must not be used to identify it. Fields within a Groupmay appear in any order or may be omitted. The CR/LF should be used to find fields and theTag used to identify it.

    ALLFIELDSAREOFMAXIMUM32 CHARACTERSLONGUNLESSOTHERWISENOTED.

    The following abbreviations are used to describe the fields:

    SEP - one SP (20H) character.

    The syntax for the Data Format of a field is:

    MS

    CHAR

    LS

    CHAR

    Two-byte ASCII representation of Hex

    value between 00 and FF (0 to 255)

    MSCHAR

    LSCHAR

    Two-byte ASCII representation of Hexvalue between 00 and FF (0 to 255)

    Tag Sep Data Format

    T I M E N N : N N : N N

    A - Alpha characters (a..z and A..Z and space) or (61H..7AH and 41H..5AH and 20H)

    N - Numeric characters (0..9 and + - .) or (30H..39H and 2BH 2DH 2EH)

    X - Printable characters (20H..7EH)

    H - Hexadecimalcharacters

    (0..9 and A..F)

    O - Other characters (CR and LF) or (0DH and 0AH)

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    Postamble:

    The postamble marks the end of the current message.

    There are 24 groups defined in the current implementation of the LH 750 WorkstationFormat. Future revisions of LH Series Workstation may include additional groups. Any futureadditions will not disrupt the order of the groups as currently defined.

    Group Definition

    The abbreviations used to identify tests the group applies to are:

    Table 4-19 lists the groups transmitted under 2A, 3A, GENS, and LH 750 Workstationformats.

    CR

    LF

    CR

    LF

    -

    -

    -

    -

    -

    -

    -

    -

    -

    -

    -

    -

    -

    -

    CR

    LF

    C CBC

    D DIFF

    R RETICS

    Table 4.19 Transmitted Groups

    Group Number Group Name Tests LH 750 LH 780 LH 500

    1 General Information C D R X, * X, * X, *

    2 CBC Parameters C X X X

    3 DIFF Count Parameters D X X X

    4 DIFF Percent Parameters D X X X

    5 Comments C D R X, * X, * X, *

    6 Definitive Flags C D R X, * X, * X, *

    7 Suspect Flags C D R X, * X, * X, *

    8 Conditional Flags C D R X, * X, * X, *

    9 Other Flags C D R X, * X, * X, *

    10 Demographics C D R X, * X, * X, *

    14 RBC Histograms C X X X

    15 PLT Histograms C X X X

    16 RETIC Parameters R X X X

    26 WBC Threshold C X X X

    27 WBC Threshold Full Histogram C X X X

    31 DIFF Latex Parameters D X X X

    32 Retic Latex Parameters R X X X

    34 Control Information C D R X X X

    35 Control CBC Parameters C X X X

    36 Control DIFF Count Parameters D X X X

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    Details of Group fields are defined in the following sections.

    General Information Group FieldsTable 4.20identifies the field names, test types, and applicable format of General Informationtransmissions.

    37 Control DIFF Percent

    Parameters

    D X X X

    38 Control Retics Parameters R X X X

    50 DIFF Graphics D X X X

    51 Retic Graphics R X X X

    53 Research Suspect Messages C D R X,

    54 Research Population Data D R X,

    X = Supported by the LH 700 Seriies, Workstation format.

    * = Indicates that group information will not be repeated with every test.

    Table 4.19 Transmitted Groups

    Group Number Group Name Tests LH 750 LH 780 LH 500

    Table 4.20 General Information -- Group 1

    Field Name Test Type LH 750 LH 780 LH 500

    Run Date C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    Run Time C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    Sample Age C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    Sample ID C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    Pre Assigned Sample ID C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    Cassette / Position C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    Pre Assigned Cassette / Position C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    Sample ID Status C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    Cassette / Position Status C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    Sample Status C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    Instrument ID C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    Operator ID C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R

    Dilution Factor C ~ C C C

    Body Fluid C ~ C C

    Stat C, CD, CDR, CR, R C, CD, CDR, CR, R

    ~ = New for the LH 750 Workstation format.

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    Run Date Field:

    Run Time Field:

    Sample Age Field:

    Sample ID Field:

    Tag Data Format Data Length Comments

    D A T E N N / N N / N N 8 bytes Run Date.

    Output ismonth/day/year,regardless of thesystem dateconfiguration.

    Tag Data Format Data Length Comments

    T I M E N N : N N : N N 8 bytes Run Time. Output is on24 hours, regardless ofthe system timeconfiguration. Timeoutput ishours:minutes:seconds.

    Tag Data Format Data Length Comments

    S A N N N N : N N : N N 11 bytes Age of sample at runtime. Format is DDHH:MM:SS. Computedfrom Draw Date and

    Draw Time. This fieldwill not appear if drawdate and time are invalidor after run date andtime.

    Tag Data Format Data Length Comments

    I D 1 X X X X X X X X 16 bytes Bar code read on tubeby instrument ataspiration time, entered

    on the Diluter keypadfor Manual modeaspiration, or IDgenerated byAuto-numberingfeature.

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    Pre Assigned Sample ID Field:

    Cassette/Position Field:

    Pre-Assigned Cassette/Position Field:

    Sample ID Status Field:

    Tag Data Format Data Length Comments

    P I D 1 X X X X X X 16 bytes PRE ASSIGNED

    SAMPLE ID

    Tag Data Format Data Length Comments

    C A S S P O S A C C C C P P 7 bytes CASS/POS output iscassette number (C)/cassette position (P).Data values for 'A' are:

    A for Automatic. M for Manual.

    Tag Data Format Data Length Comments

    P C A S S P O S C C C C P P 6 bytes CASS/POS enteredthrough ToDo list.Format is cassettenumber (C)/ cassetteposition (P). Thesample mode is notspecified atpreassignment time.

    Tag Data Format Data Length Comments

    I D 1 S T A T U S A 1 byte Data Values: P - positive ID E - edited ID N - Auto-numbered ID (20H) - not edited,not auto-numbered andnot part of positive

    identification scheme.Precedence order is: E,N, P and (20H).

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    Cassette/Position Status Field:

    Sample Status:

    Instrument ID:

    Operator ID:

    Dilution Factor::

    Tag Data Format Data Length Comments

    C / P S T A T U S A 1 byte Data Values:

    P - positive ID E - edited ID (20H) - not edited andnot part of positiveidentification scheme.Precedence order is: E,P and (20H).

    Tag Data Format Data Length Comments

    S A S T A T U S X X X X X 8 bytes See Table 4.21.

    Tag Data Format Data Length Comments

    I N S T A A A A A 16 characters Identifies the LH 750instrument this samplewas run on.

    Tag Data Format Data Length Comments

    O P R A A A A A 14 characters Identifies the userlogged on the LH 750Workstation at the timethis sample run wasanalyzed.

    Tag Data Format Data Length Comments

    D F A C T N N N N N 5 bytes Identifies amount by

    volume by whichsample is diluted.Thisfield is alwaystransmitted. All have thedefault value of 1.0when used. The factor isapplied to transmittedparameter values.

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    Body Fluid::

    Stat::

    Note: On Japanese and Chinese systems, if the Enable DBCS Transmission option isenabled, the characters may be in combination of single and double byte characters, using theDBCS format.

    Tag Data Format Data Length Comments

    B O D F A A A A A A 16 bytes This field is only

    transmitted when thesample is a body fluidand then only if EnableBody Fluid Tag inSystem Setup -(Communication) ischecked. Current data isalways Body Fluid.The parametersdisplayed are WBC, andRBC.(For LH 700 Series)

    Tag Data Format Data Length Comments

    S T A 1 byte Value: S indicates thesample has beenassigned a higherpriority as a Stat(For LH 780 Only)

    Table 4.21 Possible Values for Sample Status

    Possible Value LH Series Comments

    PART ASP X Partial aspiration

    NO READ X Bar code not read

    NO MATCH X Match not found in ToDo list

    MISMATCH X Non positive ID mismatch

    INC COL X Incomplete collation

    COMPLETE X No ToDo List errors

    .

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    CBC Parameters Group Fields

    Table 4.22identifies the field names, test types, and applicable format of CBC Parametertransmissions.

    Each field in the CBC Parameters Group will have the following format:

    Result Format: **

    The parameter result will be transmitted in the Reporting Units with the extra digit criteria

    selected on the LH Series Workstation.

    Numeric Data: **

    If the numeric data of each format (for example, xx.xx) does not contain a decimal number,then it contains one of the following:

    Table 4.22 CBC Parameters -- Group 2

    Field Name Test Type LH 750 LH 780 LH 500

    WBC C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, Edited

    RBC C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, Edited

    HGB C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, Edited

    HCT C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, Edited

    MCV C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, Edited

    MCH C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, Edited

    MCHC C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, Edited

    RDW C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, Edited

    PLT C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, Edited

    PCT C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, Edited

    MPV C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, Edited

    PDW C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited C, CD, Edited

    UWBC C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited

    RDWSD C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited

    MAF C, CD, CDR, CR, Edited C, CD, CDR, CR, Edited

    Tag Data Format Data Length Comments

    A A A A X X X X A A A A See DataFormatcolumn.

    See Below.**

    Result Comments

    . . . . . Incomplete computation

    16 char numericdata max.

    4 char flagdata max.

    1 char separator min.

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    Flag Data: **

    The flag data characters for the CBC Parameter Group Fields are as follows in Table 4.23:

    DIFF Count Parameters Group Fields

    Table 4.24identifies the field names, test types, and applicabl