lesson 21 enzymes
TRANSCRIPT
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Unit 5 - Enzymes
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Learning Objectives
• Define the term catalyst.• Describe what an enzyme is.• Describe why enzymes are important.• Describe how enzymes work.• Describe the effect of pH and temperature on
enzyme activity.
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Catalysts• A catalyst is a substance that speeds up the rate of a
chemical reaction but is not itself changed by the reaction.
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hydrogen peroxide
Hydrogen peroxide breaks down to water and oxygen
The escaping oxygen causes the foaming
2H2O2 2H2O O2+
water + oxygenmanganese oxide
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• They occur inside cells or are secreted by the cells.• Catalase is the enzyme that catalyses the break down
of hydrogen peroxide.
Enzymes act as biological catalysts
Catalase
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• Proteins are long molecules that are folded into a specific shape.
catalase amylase trypsinpepsin
Enzymes are proteins
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The part of an enzyme where the reaction occurs is known as the active site
Active site:
Enzyme
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The molecule the enzyme acts on is known as the substrate molecule
Active site:The site on the enzyme where the reaction occurs
Enzyme
Substrate molecules
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The molecule the enzyme produces is known as the product molecule
Active site:The site on the enzyme where the reaction occurs
Enzyme
Product molecules
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The enzyme can be re-used
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• The lock and key hypothesis states that the active site specifically matches the shape of the substrate molecule
enzyme
Each enzyme is specific to one substrate molecule or type of molecule
active site
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• At low temperatures enzyme controlled reactions go slowly because the molecules have low kinetic energy.
The rate of an enzyme controlled reaction is affected by temperature
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But this only occurs up to the optimum temperature (usually about 40oC)
The temperature at which the rate of reaction is fastest is known as the optimum temperature
When temperature increases the reaction also increases as the molecules have more kinetic energy
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After the optimum temperature the heat causes the enzyme to denature
The enzyme changes shape and the active site no longer matches the shape of the substrate molecule
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Rates of enzyme reactions can be measured by recording the time for a substrate to disappear or a product appears
protein polypeptidestrypsin
white clear
Controlled variables:
•Volume and concentration of substrate (milk)•Volume and concentration of enzyme (trypsin)•pH (controlled by buffers)•Temperature
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RateOf Reaction
Temperature/oC0 10 20 30 40 50 60 70
Optimum temperature
Enzyme is denaturing
Rate of reaction of an enzyme reaction changes at different temperatures
Molecules gain kinetic energy
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• Enzymes prefer to work at an optimum pH. Outside of its pH range the enzyme is denatured.
RateOf Reaction
pH
1 2 3 4 5 6 7 8 9 10 11 12
pepsin amylase
The activity and shape of enzymes is also affected by pH
Optimum pH
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Enzymes are used in biological washing powders
• Proteases break down the coloured, insoluble proteins that cause stains to smaller, colourless soluble polypeptides.
• Can wash at lower temperatures
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Enzymes are used in the food industry
• Pectinase break down substances in apple cell walls and enable greater juice extraction.
Lactase breaks down lactose in milk into glucose and galactose. This makes milk drinkable for lactose intolerant people.
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Enzymes are used in seed germination
starch
embryo plantamylasesecreted
maltoseabsorbed
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Summary
• Keywords:
enzyme
active sitesubstrate product
denaturetemperature
pH
optimum
catalase amylase
trypsin pepsinpectinase
lactase
protease
catalyst catalyse protein