lecture 9 some notes about entropy molecular biology continued
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Lecture 9 Some notes about entropy Molecular Biology continued How does genetics help us to find interesting objects and find relationships between them?. There will be NO LECTURE on Tue, March 3. The Gibbs free energy:. The process A → B will go spontaneously if. The ENTROPY. - PowerPoint PPT PresentationTRANSCRIPT
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Lecture 9
Some notes about entropy
Molecular Biology continued
How does genetics help us to find interesting objects and find relationships between them?
There will be NO LECTURE on Tue, March 3
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TSHG
The Gibbs free energy:
STHG
0 BAG
The process A→B will go spontaneously if
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WkS ln
W = multiplicity of microscopic degrees of freedom (# of microstates)
B
A
B
AT
CpdT
T
dQS
Classical definition(from analysis of steam machines)
Statistical definition
0T
dQ
A
B
The ENTROPY
k – Boltzmann constant
V
P
T
dQdS
dQ – heat imparted to the system by the surroundings
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t
i
ii ppk
S
1
ln
1 2 3 4 5 1 2 3 4 5 1 2 3 4 5
S = maxS = min
n
lattice position
Show this!
WkS ln
p – probabilities of states
What if microstates are occupied unequally? How to write the entropy?
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pdVdTCdQ V
Classical property of entropy:
dVV
R
T
dTC
T
dQdS V
dVV
RTdTCdQ V
RTpV
for a mole of ideal gas:
integrating
VRTCS V lnln
1
2
1
221 lnln
V
VR
T
TCS
V
or
( = integration constant)
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2
1
1
2 lnlnC
CR
V
VRS
At constant T and n
V
nC
so, entropy logarithmically depends on concentration
1
2
1
2 lnlnV
VR
T
TCS V
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If perfect Watson-Crick pairing was strictly enforced, then every organism should have no less than 61 different tRNAs. Many creatures get away with a considerably smaller variety because of the ‘promiscuity’ or ‘wobble’ at the third position in many codons.
Phe codons UUU and UUC (5’-3’) can both be recognized by the tRNA that has GAA (5’-3’) anticodon
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T. thermofilus 70S ribosome (M. Yusupov et al., 2001)
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Low-resolution (EM) model of E. coli 70S ribosome
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The assembly of a functional ribosome starts with initiation factors elF6 and elF3
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Methionyl-tRNAiMet recognizes the AUG start codon before mRNA is loaded
There are two types of Met tRNA: Initiation tRNA can bind only to the P siteMet tRNA used in synthesis can bind to A site and than translocate
E site P site A site
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Peptyidyl transferase reaction
Translocation
Binding of incoming amino acyl tRNA
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Termination is assisted by RFs(release factors)
UAA – stop codon
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Circular structure of mRNA increases the translation efficiency
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The replication fork: leading strand and lagging strand
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LargeT = helicase
Pol = priming DNA polymerase
Pol = DNA polymerase
PCNA - displaces Pol
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Why GENETICS? (Chapter 5)
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X X
In Haploid organisms complementig genes can be expressed on extra-chromosomal elements (plasmids), i.e. “in trans”
Diploid organisms
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Generation of temperature-sensitive mutations
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Tes
ting
mut
ants
for
com
plem
enta
tion
and
sort
ing
them
into
com
plem
enta
tion
grou
ps
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