lateral plate mesoderm and endoderm
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BIOL 370 – Developmental Biology Topic #15. Lateral Plate Mesoderm and Endoderm. Lange. Figure 12.1 Mesodermal development in frog and chick embryos. Notice here how if the chicken is removed from the large yolk (c ), that it will develop in a circular pattern like the frog. - PowerPoint PPT PresentationTRANSCRIPT
Lateral Plate Mesoderm and Endoderm
Lange
BIOL 370 – Developmental Biology
Topic #15
Figure 12.1 Mesodermal development in frog and chick embryos
Notice here how if the chicken is removed from the large yolk (c ), that it will develop in a circular pattern like the frog.
Figure 12.2 Overview of heart development
Heart development comparing chick and mouse.
Heart field formation is a major step in heart development.
The first heart field forms a primary aspect of the left ventricle, whereas the second heart field forms the primary portions of the right ventricle and the two atria in organisms with a four chambered heart.
Figure 12.3 Model of inductive interactions involving the BMP and Wnt pathways that form the boundaries of the cardiogenic mesoderm
In this model, please note how the lateral plate mesoderm has two regions with two different, but related outcomes. The anterior will become the heart whereas the posterior will become the blood & vessels. Also notice how Wnt, Noggin, and BMP affect development.
Figure 12.4 Formation of chick heart from splanchnic lateral plate mesoderm
• The endocardium is the innermost layer of tissue that lines the chambers of the heart.
• Its cells are similar to the endothelial cells that line blood vessels.
• The endocardium also provides protection to the valves and heart chambers.
Figure 12.5 Migration of heart primordia
• In (a) we see what is called cardia bifida which is a period where the embryo has “two” hearts because there is no interconnection (this is a chicken).
• (b) & (c) show zebrafish, but (c) is a mutant form called miles apart (for obvious reasons)
• (d) & (e) show mouse with (d) showing heart fusion and (e) showing a Foxp4 deficient mouse
MCPs are multipotent cardiovascular progenitors. They develop into:•endocardium - the innermost layer of tissue that lines the chambers of the heart•endothelium - the thin layer of cells that lines the interior surface of blood vessels and lymphatic vessels•smooth muscle•cardiomyocytes (cardiac muscle cells)
Figure 12.8 Cardiac looping and chamber formation (Part 1)
Human heart formation and looping occurs during the 3rd to 4th week after fertilization.
*** Also notice how the aortic sac tissues at day 21 become both the aortic sac and pulmonary arteries by birth as we talked about in the prior chapter.
Figure 12.8 Cardiac looping and chamber formation (Part 2)
• Notice how significant a change has occurred between stage 9 and stage 10 chick embryo (b & c)
• (d & e) both show the mouse heart and show through selective staining how specificity of the atria and ventricles have already occurred (due to differences in their myosin proteins)
Figure 12.9 Formation of the chambers and valves of the heart
Human Anatomy and Physiology, 7eby Elaine Marieb & Katja Hoehn
Copyright © 2007 Pearson Education, Inc.,publishing as Benjamin Cummings.
Three examples of congenital heart defects
Occurs inabout 1 in every500 births
Occurs inabout 1 in every1500 births
Narrowedaorta
Occurs inabout 1 in every2000 births
Ventricular septal defect.The superior part of the inter-ventricular septum fails to form;thus, blood mixes betweenthe two ventricles, but becausethe left ventricle is stronger, more blood is shunted fromleft to right.
(a) Coarctation of the aorta.A part of the aorta is narrowed,increasing the workload onthe left ventricle.
(b) Tetralogy of Fallot. Multiple defects (tetra = four): Pulmonary trunk too narrow and pulmonary valve stenosed, resulting in a hypertrophied right ventricle; ventricular septal defect; aorta opens from both ventricles; wall of right ventricle thickened from overwork.
(c)
Figure 12.10 Embryonic circulatory systems
Lungs and intestines are not functional in the adult sense at embryonic stages. Placental nourishment (including oxygen) necessitate some interesting differences:
•Vitelline veins serve to bring nourshment in shell encased eggs (like in birds and reptiles)
•Placental veins (umbilical vein) brings nourishment in mammals
•The allantoic artery in shell encased bird and reptile eggs will carry wastes away from the embryo
•Placental artery (umbilical artery) carries wastes away from the embryo in mammals
Levels of protein structure.
Secondary structure:The primary chain formsspirals (-helices) andsheets (-sheets).
Tertiary structure:Superimposed on secondary structure.
-Helices and/or -sheets are folded upto form a compact globular moleculeheld together by intramolecular bonds.
Quaternary structure:Two or more polypeptide chains, eachwith its own tertiary structure, combineto form a functional protein.
Tertiary structure of prealbumin(transthyretin), a protein thattransports the thyroid hormonethyroxine in serum and cerebro-spinal fluid.
Quaternary structure of afunctional prealbumin molecule.Two identical prealbumin subunitsjoin head to tail to form the dimer.
Amino acid Amino acid Amino acid Amino acid Amino acid
-Helix: The primary chain is coiledto form a spiral structure, which isstabilized by hydrogen bonds.
-Sheet: The primary chain “zig-zags” backand forth forming a “pleated” sheet. Adjacentstrands are held together by hydrogen bonds.
(a) Primary structure: The sequence of amino acids forms the polypeptide chain.
(b)
(c)
(d)
An example of the progression in complexity of structure in proteins with the final quaternary structure being that of hemoglobin.
Figure 12.11 Adult and fetal hemoglobin molecules differ in their globin subunits
When you examine fetal hemoglobin, you see that the protein differences (fetal has the y form and the adult has the B form) lead the fetal RBCs to have a higher oxygen saturation at any state of oxygen pressure.
Why is this of value?
Note that BPG (bisphosphoglycergic acid) is a molecule which is lower in fetal hemoglobin than in materinal hemoglobin.
This lower interaction gives the higher affinity for fetal hemoglobin.
Figure 12.12 Redirection of human blood flow at birth
• The ductus arteriosus is squeezed shut due to pressure changes from the expanding lungs
• The embryological foramen ovale (not to be confused with the one in the adult skull) of the heart also closes due to the pressure changes.
Figure 12.13 Aortic arches of the human embryo
Aortic arches at day 29 further develop into the leading arteries associated with the aorta and by day 56.
Some Major Blood Vessels in the Human Body
Human Anatomy and Physiology, 7eby Elaine Marieb & Katja Hoehn
Copyright © 2007 Pearson Education, Inc.,publishing as Benjamin Cummings.
The lymphatic system
Cervicalnodes
Entrance ofright lymphaticduct into rightsubclavian vein
Internaljugular veinEntrance of thoracicduct into leftsubclavian veinThoracic duct
Cisterna chyli
Lymphaticcollectingvessels
Axillarynodes
Inguinalnodes
Regionallymph nodes:
Aorta
(a)
Human Anatomy and Physiology, 7eby Elaine Marieb & Katja Hoehn
Copyright © 2007 Pearson Education, Inc.,publishing as Benjamin Cummings.
Distribution and special structural features of lymphatic capillaries
Venoussystem
Arterialsystem
Heart
Lymph ductLymph trunk
Lymph nodeLymphaticsystem
Lymphaticcollecting
vessels,with
valves
Lymphaticcapillary
Bloodcapillaries
Tissue cellBloodcapillaries
Tissue fluid
Venule Arteriole
Lymphatic capillary
Loose connective tissue around capillaries
Fibroblast in looseconnective tissue
Flaplike minivalve
Endothelialcell
Filamentsanchored toconnectivetissue
(a)
(b)
Figure 12.14 Vasculogenesis and angiogenesis
• Vasculogeneis development of new (oiginal) blood vessels• Angiogeneisis the process through which new blood vessels form from pre-
existing vessels
Florence Rena Sabin – much of her work involved the discovery of the pathways of blood vessel and lymphatic vessel genesis
Her most famous work:
“Studies on the origin of blood vessels and of red corpuscles as seen in the living blastoderm of the chick during the second day of incubation”
Journal: Contributions to Embryology
Volume 9, 213-262, 1920
Figure 12.15 Vasculogenesis (Part 1)
Vasculogenesis – notice how there is blood island formation where primitive blood cells are being made which are derived from undifferentiated mechenchyme. This is very different than in RBC production in the neonate or adult.
Figure 12.16 The lumen, or central space, in the vascular tubes is formed by the fusion of intracellular vacuoles
Lumen formation in vessels:
•Vacuole accumulation occurs in lumen cells (endocytosis)
•Vacuoles merge forming “megavacuoles”
•Continued fusion leads eventually to the lumen being formed.
Figure 12.17 VEGF and its receptors in mouse embryos
The VEGF mutant lacks blood vessel development with the yolk sac, and therefore is miscarried.
Figure 12.18 Roles of ephrin and Eph receptors during angiogenesis
Eph receptors predominate venous vessels, whereas ephrin-b2 receptors predominate arterial vessels. To encourage angiogenic modeling of capillaries, there is some (as yet unclear) ephrin/Eph interaction that allows formation of these capillary connections.
Figure 12.20 Blood vessel formation in the chick blastoderm
One theory explaining blood vessel formation involves the use of VEGF (vascular endothelial growth factor) which may occur as a gradient promoting vessel formation in areas with higher concentration.
Figure 12.22 VEGF-C is critical for the formation of lymphatic vessels
A “C” form of VEGF (VEGF-C) is the growth factor for the formation of lymph vessels. Notice the pronounced edema in the mouse embryo that is VEGF-C deficient.
Figure 12.23 Sources of blood cells to adult bone marrow
Hematopoiesis – the formation of blood cells is primarily in the red bone marrow in the adult, but embryologically it can occur in diverse places including the liver, the yolk sac and placenta.
Hematopoietic stem cells near and around osteoblast cells can differentiate into various blood cell components.
Figure 12.24 A model for the origin of mammalian blood and lymphoid cells
Human Anatomy and Physiology, 7eby Elaine Marieb & Katja Hoehn
Copyright © 2007 Pearson Education, Inc.,publishing as Benjamin Cummings.
Erythropoiesis: genesis of red blood cells
Stem cell
Hemocytoblast ProerythroblastEarlyerythroblast
Lateerythroblast Normoblast
Phase 1Ribosome synthesis
Phase 2Hemoglobin accumulation
Phase 3Ejection of nucleus
Reticulocyte Erythrocyte
Committed cell Developmental pathway
Human Anatomy and Physiology, 7eby Elaine Marieb & Katja Hoehn
Copyright © 2007 Pearson Education, Inc.,publishing as Benjamin Cummings.
Leukocyte formation
Hemocytoblast
Myeloid stem cell Lymphoid stem cell
Myeloblast MyeloblastMyeloblast Lymphoblast
Stem cells
Committedcells
Promyelocyte PromyelocytePromyelocyte Promonocyte Prolymphocyte
Eosinophilicmyelocyte
Neutrophilicmyelocyte
Basophilicmyelocyte
Eosinophilicband cells
Neutrophilicband cells
Basophilicband cells
Develop-mentalpathway
Eosinophils NeutrophilsBasophils
Granular leukocytesPlasma cells
Some become
Monocytes Lymphocytes
Macrophages (tissues)
Agranularleukocytes
Some become
(a) (b) (c) (d) (e)
Human Anatomy and Physiology, 7eby Elaine Marieb & Katja Hoehn
Copyright © 2007 Pearson Education, Inc.,publishing as Benjamin Cummings.
Genesis of platelets
Stem cell Developmental pathway
Hemocytoblast Megakaryoblast Promegakaryocyte Megakaryocyte Platelets
Figure 12.26 Endodermal folding during early human development
Notice the differential cuts and how they show organization of the structures that can be visualized in 3D.
Figure 12.27 Formation of glandular primordia from the pharyngeal pouches
Notice how pharyngeal arches develop into:•1 tympanic cavity •2 tonsils•3 parathyroid gland (dorsal) and (ventral) the thymus •4 more parathyroid gland.
•Note that the thyroid is not part of any pharyngeal arch.
Figure 12.28 Regional specification of the gut endoderm and splanchnic mesoderm through reciprocal interactions (Part 1)
• cSox2 - • Pdx1 pancreatic and
duodenal homeobox 1• Hox homeobox• cdxC caudal gene C• cdxA caudal gene A
Figure 12.29 Pancreatic development in humans
Figure 12.33 Partitioning of the foregut into the esophagus and respiratory diverticulum during the third and fourth weeks of human gestation
Figure 12.34 Wnt signaling is critical for separation of the trachea and early differentiation of the lung
Figure 12.35 The immune system relays a signal from the embryonic lung
End.