laser desorption supersonic jet spectroscopy of hydrated tyrosine june 18th, 2013 hikari oba tokyo...
TRANSCRIPT
Laser desorption supersonic jet spectroscopy of hydrated tyrosine
June 18th, 2013
Hikari OBA
Tokyo Tech, Japan
Cooperative effect of hydrogen bond
*R. Nathaniel Pribble, et al.J. Chem. Phys., 106, 2145 (1997).
Bz-(MeOH)1
Bz-(MeOH)2
Why tyrosine-water cluster ?
Phenylalanine X
Tyrosine XR
Dopa
different ! almost same
[1] L. C. Snoek, et al., CPL, 2000 321 49. [2] Y. Shimozono, et al., PCCP, 2013, 15, 5163. [3] S. Ishiuchi, et al., PCCP, 2011, 13, 7812
Why tyrosine-water cluster ?
Stronger H-bond
To Investigate cooperative effect of separate hydrogen bond
more enhanced ?
Dopa
weaker H-bond
Tyr XR-(H2O)1
Assignment of water binding site
free OHH-bonded OH
* T. Ebata, et al., International Journal of Mass Spectrometry and Ion Processes, 1996, 159, 111.
*
A)
B)
Tyr-(H2O)1
Phenol
Phenol-(H2O)1
*
Assignment of conformation of tyrosine-(H2O)1
Tyr XR-(H2O)1
Tyr XR
Tyr XR-(H2O)1
[1]
[1] Y. Shimozono, et al., PCCP, 2013, 15, 5163
phenolic OH bound
Phe X
Tyr XR
Dopa
Tyr XR-(H2O)1
Comparison of the effect on hydrogen bond in amino acid
stronger H-bond
weaker H-bond
This work !
?
Summary
REMPI and IR dip spectra of tyrosine-(H2O)1 were measured.
Direction of dipole moments is important to enhance the hydrogen bond strength in cooperative effect.
Acknowledgment
Merci beaucoup !
Ms. Yoko Shimozono
Dr. Shun-ichi Ishiuchi
Prof. Masaaki Fujii Chemical Resources Lab., Tokyo Tech.
Chemical Resources Lab., Tokyo Tech.
Chemical Resources Lab., Tokyo Tech.
Dr. Pierre Çarçabal Institut des Sciences Moléculaires d’Orsay, U. Paris-Sud.