larval white sturgeon - rmpc...larval white sturgeon substrate rearing leads to improved growth,...

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Page 1: Larval white sturgeon - RMPC...Larval white sturgeon Substrate rearing leads to improved growth, survival, and physiology. Contact Info Marcus Boucher marcus.boucher@gofishbc.com marcusboucher@gmail.com
Page 2: Larval white sturgeon - RMPC...Larval white sturgeon Substrate rearing leads to improved growth, survival, and physiology. Contact Info Marcus Boucher marcus.boucher@gofishbc.com marcusboucher@gmail.com

Larval white sturgeonSubstrate rearing leads to improved growth, survival, and physiology

Page 3: Larval white sturgeon - RMPC...Larval white sturgeon Substrate rearing leads to improved growth, survival, and physiology. Contact Info Marcus Boucher marcus.boucher@gofishbc.com marcusboucher@gmail.com

Contact Info

� Marcus Boucher

[email protected]

[email protected]

� 778-245-9276 (cell)

� 604-855-4720 (work)

Page 4: Larval white sturgeon - RMPC...Larval white sturgeon Substrate rearing leads to improved growth, survival, and physiology. Contact Info Marcus Boucher marcus.boucher@gofishbc.com marcusboucher@gmail.com

Overview

� Results of gravel rearing experiments

� Considerations and challenges

� How it applies to conservation aquaculture

� Future options for research?

Page 5: Larval white sturgeon - RMPC...Larval white sturgeon Substrate rearing leads to improved growth, survival, and physiology. Contact Info Marcus Boucher marcus.boucher@gofishbc.com marcusboucher@gmail.com

Why substrate?� Less swimming when rearing in substrate

� Yolk supplies all energy for growth, development,

and metabolism

� Amount of yolk is finite

� The more yolk resources that go to one process

the less are available for others

� Larvae in substrates move less -> more yolk

available for growth

Page 6: Larval white sturgeon - RMPC...Larval white sturgeon Substrate rearing leads to improved growth, survival, and physiology. Contact Info Marcus Boucher marcus.boucher@gofishbc.com marcusboucher@gmail.com

Experimental SetupTwo experiments:1)Two temperature – gravel vs no gravel

Growth, survival, yolk utilization

2)Gravel vs Artificial substrate vs no substrate

Growth and physiology

Page 7: Larval white sturgeon - RMPC...Larval white sturgeon Substrate rearing leads to improved growth, survival, and physiology. Contact Info Marcus Boucher marcus.boucher@gofishbc.com marcusboucher@gmail.com

Gravel

13.5oC

Bare

13.5oC

Gravel

17.5oC

Gravel

13.5oC

Bare

17.5oC

Bare

13.5oC

Growth at 46 days post hatch

Page 8: Larval white sturgeon - RMPC...Larval white sturgeon Substrate rearing leads to improved growth, survival, and physiology. Contact Info Marcus Boucher marcus.boucher@gofishbc.com marcusboucher@gmail.com

Growth

Page 9: Larval white sturgeon - RMPC...Larval white sturgeon Substrate rearing leads to improved growth, survival, and physiology. Contact Info Marcus Boucher marcus.boucher@gofishbc.com marcusboucher@gmail.com

Growth

Gravel

No gravel

Page 10: Larval white sturgeon - RMPC...Larval white sturgeon Substrate rearing leads to improved growth, survival, and physiology. Contact Info Marcus Boucher marcus.boucher@gofishbc.com marcusboucher@gmail.com

Mortality at first feeding

•Higher mortality at onset of feed – greater in warmer water

Page 11: Larval white sturgeon - RMPC...Larval white sturgeon Substrate rearing leads to improved growth, survival, and physiology. Contact Info Marcus Boucher marcus.boucher@gofishbc.com marcusboucher@gmail.com

Survival

20% greater survival in gravelGreatest survival at lower temperature in gravel

Page 12: Larval white sturgeon - RMPC...Larval white sturgeon Substrate rearing leads to improved growth, survival, and physiology. Contact Info Marcus Boucher marcus.boucher@gofishbc.com marcusboucher@gmail.com

Physiology

� How much a fish can raise it’s metabolic rate to deal with a challenge

� Capacity for growth and other energetic processes

Active Rest

- = Metabolic

Scope

Page 13: Larval white sturgeon - RMPC...Larval white sturgeon Substrate rearing leads to improved growth, survival, and physiology. Contact Info Marcus Boucher marcus.boucher@gofishbc.com marcusboucher@gmail.com

Physiology

5 10 15 20 25

Mean

metabolic sco

pe

ug O

2 / g / hr

0

50

100

150

200

250

300Gravel

BareMetabolic sco

pe / larvae

Days post hatch

No substrate – lower capacity for growth and other

energetic processes

Page 14: Larval white sturgeon - RMPC...Larval white sturgeon Substrate rearing leads to improved growth, survival, and physiology. Contact Info Marcus Boucher marcus.boucher@gofishbc.com marcusboucher@gmail.com

Energy Reserves

In short, fish reared without gravel were out of gas…

8 dph 16 dph

Gravel

Bare

Page 15: Larval white sturgeon - RMPC...Larval white sturgeon Substrate rearing leads to improved growth, survival, and physiology. Contact Info Marcus Boucher marcus.boucher@gofishbc.com marcusboucher@gmail.com

How does this apply to the hatchery?

� Substrate could be used to increase growth and survival

� Gravel is not ideal for hatchery use!

� What else is out there?

Page 16: Larval white sturgeon - RMPC...Larval white sturgeon Substrate rearing leads to improved growth, survival, and physiology. Contact Info Marcus Boucher marcus.boucher@gofishbc.com marcusboucher@gmail.com

Artificial Substrate

1” sinking Bio-spheres

Page 17: Larval white sturgeon - RMPC...Larval white sturgeon Substrate rearing leads to improved growth, survival, and physiology. Contact Info Marcus Boucher marcus.boucher@gofishbc.com marcusboucher@gmail.com

Artificial Substrate

•No difference between gravel and Artificial substrates

Substrate

No Substrate

•Bio-spheres may be a suitable replacement for gravel

Page 18: Larval white sturgeon - RMPC...Larval white sturgeon Substrate rearing leads to improved growth, survival, and physiology. Contact Info Marcus Boucher marcus.boucher@gofishbc.com marcusboucher@gmail.com

Benefits of Substrate

� Faster growth

� Lower mortality at first feeding

� Greater overall survival

� Improved physiological condition

Page 19: Larval white sturgeon - RMPC...Larval white sturgeon Substrate rearing leads to improved growth, survival, and physiology. Contact Info Marcus Boucher marcus.boucher@gofishbc.com marcusboucher@gmail.com

Some considerations…� Using substrate is more time consuming

� However, only rear in substrate for a short time (300-330

ATU)

� Much less artificial substrate is required than gravel

� Greater overall survival is not our goal

� May reduce loss of some family groups = more genes

released

Page 20: Larval white sturgeon - RMPC...Larval white sturgeon Substrate rearing leads to improved growth, survival, and physiology. Contact Info Marcus Boucher marcus.boucher@gofishbc.com marcusboucher@gmail.com

Thanks!

Funding

BC Hydro

NSERC Industrial Postgraduate Scholarship program

Freshwater Fisheries Society of BC