laprep sigma

PreParative ChromatograPhy

– the sum of possibilities

12.20.2011 - 2011-EU-BrochInsert-LabCon-OnlinePlanningTool.indd - Designer: andrewh / PM: Marcb / Requestor: beckyc ww- Size: a4

2 | Vwr international | preparatiVe chroMatoGraphy

LaPrep Sigma – the new compact preparative chromatography system from vWr

Being a truly modular system with many possible configurations, having the smallest

foot print in the market, an easy to use touch screen panel and ethernet connection, it is

the ideal preparative hPLC system for modern purification.

Running on the intuitive eZChrom platform, the new laprep sigma has capabilities ranging from analytical to preparative scale, pump capacity from 1 to 600 ml/min as well as easy connection to a fully automated fraction collector. Along with the option of a single or multiple wavelength uV detector and options for autosampler or single injection sample delivery, the new laprep sigma from VWR is an excellent choice for any prep lab whatever the configuration requirements.

from bench use in research laboratories to scale up laboratories: the range of capacity of the pump heads, with maxmimum flow rate for each solvent pump at 50, 200 and 600 ml/min, means that it is highly flexible for use in all chemistry laboratories. the flexible flow rate capacity coupled with the modular design means that it can be a really cost effective solution.

adapt LaPrep ∑ to your application• Versatile, modular system• stand-alone mode for all modules• pump heads with titanium inlays for all sample contact components• Double piston pump with electronic residual pulsation suppressor and active piston seal wash• multi-purpose valves, pumps with titanium inlays• optional combination with autosampler or fraction collector• manual or fully automated control• small footprint

LaPrep∑ - the Sum of PoSSiBiLitieS

preparatiVe chroMatoGraphy | Vwr international | 3

Pumpseach pump head is manufactured using titanium for all sample contact components. this makes laprep sigma pumps suitable for use with biological samples where a high salt concentration buffer would otherwise corrode stainless steel. All pumps are double piston with electronic residual pulsation suppressor and active piston seal wash which are fitted at the site of manufacture and tested before shipping.

the capacity range of the pump heads, with maximum flow rate for each at 50, 200 and 600 ml/min, means that it is highly flexible for use in all chemistry laboratories from bench use in research laboratories to scale up laboratories. As shown in table 1, with its range of flow rates, the laprep sigma covers loading capacity of up to 5 g and loading volume of 80 ml. this flexible flow rate capacity coupled with the modular design means it can be a really cost effective solution.

table 1: flow rate increments of the vWr LaPrep SigmaSolvent delivery Column dimension

LxD (mm) Typical flow rates

(ml/min) Loading

capacity (mg) Loading

volume (µl) Solvent delivery

VWR Hitachi Chromaster HPLC system

250 - 4 1 5 5 - 80

250 - 10 6 30 30 - 500 VWR LaPrep Sigma Semi-Prep with 50 ml headVWR LaPrep Sigma with

200 ml head250 - 25 39 200 200 - 3.000

250 - 50 156 800 800 - 12.500 VWR LaPrep Sigma with 600 ml head400 - 100 625 5.12 5.120 - 80.000


Configuration and softwareRunning on the intuitive eZChrom platform, the new laprep sigma is a compact preparative chromatography system from VWR. being a truly modular system, with the smallest footprint in the market, an easy to use touch screen panel and ethernet connection lapre sigma is an ideal addition to any laboratory needing a flexible prep solution.

the laprep sigma has capabilities ranging from analytical to preparative scale, pump capacity from 1 to 600 ml/min, as well as easy connection to a fully automated fraction collector. Along with the option of a single or multiple wavelength uV detector and options for autosampler or single injection sample delivery, the new laprep sigma from VWR is an excellent choice for any prep lab whatever the configuration requirements.

As with all VWR’s chromatography instrumentation, the new laprep sigma is designed with robustness and durability in mind.

fraction and system control with eZChrom elite™

the laprep sigma can run in a semi-prep mode with the 50 ml/min pump. in addition, because it is controlled by eZ Chrom software it can be combined with the VWR hitachi Chromaster analytical system in order to provide a simple, easy to use semi-prep system whilst taking advantage of the Chromaster’s excellent autosampler and detector capabilities*.

the eZ Chrom software allows for straight forward peak recognition. it starts to search for substance peaks at a pre-determined time. the substance peaks are recognised by looking for criteria which fulfil the peak start and end level. this leads to a simplification of the programming needed for a complete fraction as only one line needs to be programmed for a complete fraction with the same parameters.

the peak recognition and subsequent fraction collection can clearly be seen in figure 1. the eZChrom elite™ software using the eZ prep Driver clearly displays colour coded areas of the chromatogram to represent peak collection, peak recycling or solvent recycling. figure 1 shows the “signal level / slope” command in operation for fraction collection.

Figure 1. EZChrom Elite™ showing the collection of half a peak and a full peak using the «Signal Level / Slope» command to switch the flow between waste and fraction collection vessel.

* configurations involving a mixture of Chromaster and LaPrep Sigma modules are suitable for research purposes only.


Direct control of fraction collectionit is also very simple to directly control either the fractionation valve or fraction collection via the direct control tool in eZ prep. figure 2 shows the control panel for the fractionation valve. With only a few clicks of the mouse, the flow can be diverted into the corresponding fractionation vessel until a further command diverts the flow to another collection vessel or to waste.

Figure 2. Shows direct control panel with the corresponding collected peaks in the chromatogram.

When trying to separate two or more components that elute at similar times with an overloaded column, peak recycling can be set-up, where by unresolved fractions are fed back into the system in order to undergo further separation. this is particularly suited to processes where only one fraction is of interest and a more environmentally sound separations process is being sought.

With preparative chromatographers in today’s laboratories being required to provide ever more bespoke and tailored solutions to medicinal chemists, materials chemists and chemical engineers, VWR has designed a highly flexible system to suit the vast majority of cases.

VWR can offer a complete package for preparative chromatography, including self-pack and pre-packed columns, solvents, syringes and vials.


Software, control and start-up

Cat. No. Type905-0536 Ethernet module connector For connection of LaPrep Sigma modules via ethernet

906-0079 Software EZChrom Elite™ versions 3.3.2, SP2

905-0535 Driver LaPrep Sigma driver for EZChrom Elite™ versions 3.3.2.SP2

905-0078 Interface box IF2 4-channel analogue data input for use with EZChrom Elite™, D/A - A/D interface for third party products

905-0520 Start-up kit with 1/16" tubing Contains all fittings and tubing needed for 1/16” set-up

905-0519 Start-up kit with 1/8" tubing Contains all fittings and tubing needed for 1/8” set-up


Pumps/solvent delivery options

Common to all pumpsFeatures Double piston pump (main and auxiliary piston) active piston

seal washing

Titanium inlays for all sample contact components

Electronic residual pulsation suppressor

Pressure sensor

Control LAN, RS232

Touch screen on module front

Terminal strip events, terminal strip remote

Programming 10 programmes, 9 programme links, WAKE UP programme

Cat. No. Type Technical specifications905-0502 LP1100 (50 ml) Characteristics 50 ml pump head

Flow range 0.01 - 25 ml/min at 200 bar, 0.01 - 50 ml/min at 150 bar

Extra features for the LP 1100 Static dynamic mixing chamber (350 µl volume), quaternary low pressure gradient

Programming 10 programs, 9 programme links, WAKE UP programme

Gradient Quaternary low pressure gradient, ranges 0 – 100%, 1% increments

Accuracy <1% at 0.4 to 40 ml/min

Precision <0.4% at 5 ml/min relative standard deviation

Residual pulsation <0.5% at 1 ml/min

WxDxH 242x352x165 mm plus 45 mm depth for pump head


Flow range 0.1 - 200 ml/min

Max. pressure 200 bar

Accuracy ±2% at 2 to 50 ml/min



Wxdxh 242x352x165 mm plus 45 mm depth for pump head

905-0522 LPG accessory Low pressure gradient flow rate Ternary, 10 - 220 ml/min

Material Stainless steel


Flow range 0.1 - 600 ml/min


Accuracy ±2% at 2 to 50 ml/min



WxDxH 242x352x165 mm plus 45 mm depth for pump head

905-0521 LPG accessory Low pressure gradient flow rate Binary, 10 - 1000 ml/min


Dynamic mixing chambers (advisable for all lpg high flow rate set-ups)

905-0014 Dynamic mixing chamber

Volume 5.9 Ml

Tubing 1/8” With adapter for 1/16”


Flow range Up to 250 ml/min

Max. pressure 250 Bar

905-0015 Extension for dynamic mixing chamber

Volume 4.8 Ml


Flow range Up to 250 ml/min

Max. pressure 250 Bar


assistant (sample injection)

For 1/16” capillary set-upCat. No. Type Technical specificationsmanual injection

905-0512 Manual valve Tubing 1/16”

Features 6 port, 3 channels, 2 positions

Includes Signal cable, mounting bracket

Automated injection: LaPrep Sigma Assistant

905-0514 Semi-preparative assistant

Tubing 1/16”

Feed pump Lp1000 10 ml pump head, 400 bar for details see 905-0500

Injection valve Lp2002 6 port, 3 channels, 2 positions for details see 905-0507

Fractionation valve Lp2016 17 ports, 1 channel, 17 positions for details see 905-0511

WxDxH 242x352x137 mm plus pump head and valve

Automated injection: Feed pump + injection valve

905-0500 LP1000 Characteristics 10 ml pump head

Flow range 0.001 – 9.999 ml/min


905-0501 LP1000 Characteristics 50 ml pump head



Features Double piston pump (main and auxiliary piston) active piston seal washing



Pressure sensor

Control LAN, RS232

Keys on module front (2 function keys, Start/Stop key)

Terminal strip remote

Analog out

Accuracy ±1% at 1 ml/min


WxDxH 242x352x138 mm

905-0507 LP2002, 1/16” Features Valve with drive, 6 ports, 3 channels, 2 positions

Tubing 1/16”


Sample loops for automated injection

905-0029 Tubing 1/16”

Volume 10 ml

905-0030 Tubing 1/16”

Volume 40 ml

Design note:the assistant is key to the flexibility of laprep sigma. the assistant can house the fractionation valve, injection valves and automated injection pump, meaning that minimal bench space is used and a variety of configuration options are available.


For 1/8” capillary set-upCat. No. Type Technical specificationsautomated injection: LaPrep Sigma Assistant

905-0515 Semi-preparative Assistant

Tubing 1/8”

Feed pump Feed pump LP1000, 50 ml pump head, 150 bar details see 905-0501

Injection valve LP2002 6 ports, 3 channels, 2 positions for details see 905-0508

Fractionation valve 13 ports, 1 channel, 17 positions for details see 905-0510


Automated injection: feed pump + injection valve

905-0501 LP1000 Max. pressure 150 bar

Characteristics 50 ml pump head



Features Double piston pump (main and auxiliary piston) active piston seal washing



Pressure sensor

Control LAN

RS232

Keys on module front (2 function keys, Start/Stop key)

Terminal strip remote

Analog out

Accuracy ±1% at 1 ml/min



905-0508 LP2002, 1/8” Features Valve with drive, 6 ports, 3 channels, 2 positions

Tubing 1/8”


Sample loops for automated injection

905-0031 Tubing 1/8”

Volume 10 ml

905-0032 Tubing 1/8”

Volume 40 ml

alias autosamplerCat. No. Technical specifications905-0124 Vial dimensions (cap included) Max. vial height: 47 mm, Min. vial height: 32 mm

Dispenser syringe 2500 BL syringe

Injection volume 1 µl - 10.000 µl, with 1 µl increments

Valve Valco 0.75 bore valve

Sample loop 10 ml SS sample loop, 1/8” tubing with 1/16” tubing ends and fittings (Valco)

Buffer tubing 2 ml

Needle LVS needle

assistant (sample injection) cont.


Detector options

Common featuresType Fast scanning two-beam monochromator spectrophotometer

Light source Deuterium lamp

Wavelength 190 - 750 nm

Integration time 500 ms – 12 ms, determined automatically

Control LAN, RS232

Touch screen on module front

Terminal strip: Event and remote control

Programming Time controlled: Wavelengths, events, valve control, programme links, 19 programmes, WAKE UP programme

WxDxH 242x352x169 mm plus flow cell

Cat. No. Type Technical specifications905-0505 LP3101 1-channel UV detector

Type Fast scanning two-beam monochromator spectrophotometer



Accuracy ±2 nm

Precision 0.7 nm residual standard deviation

Optical bandwidth 8 nm

Noise ±0.75 x 10 - 5 AU/h at 254 nm

Drift 1.5 x 10 - 4 AU/h at 254 nm

Linearity >2.5 AU

905-0506 LP3104 4-channel UV detector

Type Fast scanning two-beam monochromator spectrophotometer



Accuracy ±1 nm

Precision 0.4 nm residual standard deviation

Optical bandwidth <5 nm

Noise ±0.5 x 10 - 5 AU/h at 254 nm

Drift ±0.5 x 10 - 4 AU/h at 254 nm

Linearity >3 AU

Flow cell option

905-0530 Preperative flow cell

Tubing 1/8”

Path length 0.5/1.25/2 mm selectable path length

Volume 25 µl

Max. flow rate 1000 ml/min


905-0531 Semi-preparative flow cell

Tubing 1/16"

Path length 0.5 mm

Volume 3 µl



905-0529 Analytical flow cell Tubing 1/16”

Path length 3 mm

Volume 2 µl




fractionation

1/16" fractionationCat. No. Type Technical specificationsFractionation valve

905-0511 LP2016 Tubing 1/16”

Features Fractionation valve with drive, 17 ports, 1 channel, 17 positions


Fractionation using the assistant


Tubing 1/16”

Feed pump LP1000 10 ml pump head, 400 bar for details see 905-0500


Fractionation valve LP2016 17 ports, 1 channel, 17 positions for details see 905-0511


905-0517 Assistant Tubing 1/16”

Functionality 2 valves, 7 ports, 1 channel, 7 postions


1/8" fractionationCat. No. Type Technical specificationsFractionation valve

905-0509 LP2006 Tubing 1/8”

Features Valve with drive, 7 ports, 1 channel, 7 positions


905-0510 LP2012 Tubing 1/8”

Features Valve with drive, 13 ports, 1 channel, 13 positions


Fractionation using the assistant


Tubing 1/8”

Functionality Feed pump LP1000, 50 ml pump head, 150 bar for details see 905-0501


Fractionation valve LP2012 13 ports, 1 channel, 17 positions for details see 905-0510


905-0516 Assistant Tubing 1/8”

Functionality 2 valves LP2006, 7 ports, 1 channel, 7 positions


Fractionation collectorsCat. No. Type Technical specifications905-0090 Vario 2000 Flow rate <20 l/min

905-0125 Vario 4000 Flow rate <100 l/min

905-0533 Foxy R1 Stand-alone package

Flow rate <25 ml/min

Capillaries ø 1/16”

Tube ø 18 mm ext. ø

905-0534 Foxy R2 stand-alone package

Flow rate <125 ml/min

Capillaries ø 1/8”

Tube ø 18 Mm ext. ø

* further accessory options are available

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