lancefield 2011 poster virtual tour
DESCRIPTION
Virtual Tour of Poster presented at Lancefield 2011.TRANSCRIPT
The Virtual Tour
BIOLUMINESCENT IMAGING OF STREPTOCOCCUS PYOGENES IN NASOPHARYNGEAL INFECTIONFaraz Alam1, Colin Bateman1, Claire Turner1, Ken Smith2, Siouxsie Wiles1,3 & Shiranee Sriskandan1
1 Dept. of Infectious Diseases & Immunity, Imperial College London, London W12 0NN, UK. 2Dept. of Pathology & Infectious Diseases, Royal Veterinary College, Hertfordshire AL9 7TA , UK.
3 Dept. of Molecular Medicine & Pathology, University of Auckland, 1142, New Zealand.
Current Models
Live Mouse
Current Models
Live Mouse
Cull
Organs
Current Models
Live Mouse
Cull
Organs
Plate
Bacteria
Current Models
Live Mouse
Cull
Organs
Plate
Bacteria
Current Models
Single Time Point
Bact
eria
/ g
ram
of ti
ssue
Time
Live Mouse
Cull
Organs
Plate
Bacteria
Direct Culture
Direct Culture
0 7 14 210
20
40
60
80
100
Males Females
Time (days)
Per
cen
t C
arri
age
Infection Longevity
Inflammation in the Nasopharynx
Inflammation in the Nasopharynx
Turner & Sriskandan, 2007
Impact of covRS on carriage
0 5 10 15 200
20
40
60
80
100
Wild type
covRS Mutant
Days Post Inoculation
Per
cen
t C
arri
age
Impact of covRS on carriage
Airborne Transmission
Airborne Transmission
hrs
Dir
ect
Nas
al S
amp
le c
ou
nts
(cf
u)
in R
ecip
ien
t M
ice
0 20 40 60 801
10
100
1000
10000 2 seed mice per cage
3 seed mice per cage
4 seed mice per cage
Airborne Transmission
Photorhabdus luminescens
Bioluminescence Imaging
Light
Camera
Bioluminescence Imaging
Light
Camera
Bioluminescence Imaging
Bioluminescence Imaging
Camera
Light
Growth Kinetics
Growth Kinetics
Time
Opt
ical
Den
sity
Luminescence(R
LU)
0 2 4 6 8 100.0
0.5
1.0
0
5000
10000
15000
20000
Wild type
Spy0535 Mutant
Bioluminescent strain
Luminescent signal
In vivo Growth Kinetics
In vivo Growth Kinetics
cfu
/ n
ose
100
101
102
103
104
105
106
107
108
109Wild type vs Luminescent
Day 1 Day 1 Day 1Day 3 Day 3 Day 3
Wild type vs Knockout Luminescent vs Knockout
In vivo Growth Kinetics
cfu
/ n
ose
100
101
102
103
104
105
106
107
108
109Wild type vs Luminescent
Day 1 Day 1 Day 1Day 3 Day 3 Day 3
Wild type vs Control Luminescent vs Control
In vivo Growth Kinetics
cfu
/ n
ose
100
101
102
103
104
105
106
107
108
109Wild type vs Luminescent
Day 1 Day 1 Day 1Day 3 Day 3 Day 3
Wild type vs Control Luminescent vs Control
In vivo Growth Kinetics
Days Post Inoculation
Fit
nes
s re
lati
ve t
o w
ildty
pe
0 1 2 3 40
1
2
3
4
Luminescent Strain
Control strain
100 101 102 103 104 105 106 107 10810000
100000
1000000
Total CFU
To
tal F
lux (
ph
oto
ns/
seco
nd
)
Click # FMA2010062408431524 Jun 2010 8:43:15Em filter=OpenBin:M (8), FOV10, f1, 5mCamera: 13195, EEV
User: FAGroup: H494 Day 2Experiment: Animal Number: G, BLView: Dorsal
70000
60000
50000
40000
30000
20000
ImageMin = -4984.5Max = 79669p/sec/cm^2/sr
Color BarMin = 20000Max = 72038
bkg subflat-fieldedcosmic
ROI 1
Bioluminescence in the nasopharynx
FA20100813120726_SEQ
FA20100120123803
4
5
6
7
8
910
4
2
3
4
5
6
7
8
910
5
Color BarMin = 4000
Max = 1e+05
FA20100120124351 FA20100120125227
FA20100120125833 FA20100120130548 FA20100120131155
Longitudinal imaging
Longitudinal imaging
Vaccination Study
PBS
Vacc
inat
edH
eat K
illed
Bac
teria
Day 1 Day 35Day 14 Day 28
Vaccination StudyPB
S Va
ccin
ated
Hea
t Kill
ed B
acte
ria
Day 1 Day 2 Day 3 Day 4
Vaccination Study
Time Course
Days
Lu
min
esce
nce
(p
ho
ton
s/se
con
d)
0 1 2 3 4 510000
100000
1000000
Heat Killed H347PBS
Cfu
/ G
ram
Heat Killed H347 PBS100
101
102
103
104
105
106
107
108
Mucosal Transmission
Summary
Summary
Bact
eria
/ g
ram
of ti
ssue
Time
Single Time Point
Photograph/ Nasal Sample
Summary