lamda phage
TRANSCRIPT
Life cycle of λ phage
Presented by:Minhaz AhmedBBI11014Integrated M.Sc 3rd semester,MBBT,Tezpur University, Assam
CONTENTS Introduction
How genome is transferred
Lytic cycle and lysogenic cycle
Life cycle
Lytic or lysogenic?
What is lambda phage?
It is a bacterial virus, or bacteriophage , that infects the bacterial species Escherichia coli.
This virus is a temperate phage.
Temperate phages are basically bacteriophages which can choose between a lytic and lysogenic pathway of development.
SOURCE:classes.midlandstech.edu
The viral DNA exists as a separate molecule within the bacterial cell. Replicates separately from the host bacterial DNA.
Viral DNA integrates with bacterial DNA.Replicates with the host DNA
How it penetrates:
It binds to the target E. coli cell, via J protein in the tail tip that interacts with the maltoporin of E. coli and injects its linear genome.
DNAHEADTAIL
COLLARMALTOPORIN in E. coliBASE PLATE
DNA of E. coli
E. coli
TAIL FIBRE1
2
LIFE CYCLE :
SOURCE: Pearson Education 2004
Lytic or lysogenic?
1.lytic life cycle: occurs in cells with sufficient nutrients, protease activity is high, which breaks down cII.
2.Lysogenic life cycle: In cells with limited nutrients, protease activity is low, making cII stable.
Computer modeling and simulation suggest that random processes during infection drive the selection of lysis or lysogeny within individual cells.
However, recent experiments suggest that physical differences among cells, that exist prior to infection, predetermine whether a cell will lyse or become a lysogen.
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