lab report1 gametogenesis a karle150211 - napa valley...

Biology 105 – Human Biology

Laboratory Report

Session: Section:

Class Location: Days / Time:

Instructor:

Spring 2015 55244 / 61816 4 Units UVC1, 3 and 7 St. Helena F 9:00 AM – 11:50 AM LEC F 1:00 PM – 3:50 PM LAB 55244 M 9:00 AM – 11:50 AM LAB 61816 RIDDELL

Lab Report 1 Gametogenesis A Karle 150211 1

Student Author Name: Annaka Karle

Team Members Name(s):

Meagan Brandon Breanne Salmassy Susan Davidson

Student Author ID #: 0207775 Team Members ID #: 0309439, 0310518, 0337558

Lab Assignment #: 1 Team Name: 3D

Lab Assignment Title: Human Gametogenesis Date: 150211

Purposes / Objectives:

1. Observe Cheek Cells and Estimated Cell size using a Calculator 2. Observe Human Gametogenesis in prepared MS Slides via standard Light Microscopy. 3. Survey and capture illustrations and photos from the Internet of the above. 4. Measure Cell and Structure Size utilizing Field of View Calculator.

Hypothesis: NA Specimens:

1. Specimen of cheek cells form self 2. Prepared MS slides featuring a cross-section of:

A. Sperm Smear Human B. Testes C. Ovary D. Maturing, Follicle with Human Oocytes E. Corpus Luteum

. Methods / Tools / Instrumentation / Procedures:

1. Prepared a MS sample of cheek cells from self and observed via standard Light Microscopy 2. Standard Light microscope with 100, 400 and 1000 Magnification 3. View cheek cells at 100X and 400X 4. View Human sperm smear, and view ovary maturing and follicle sections in human ovary at

100X, 400X, and 1000X.


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Results ▪ Viewing the target specimens and structures with the standard light microscopes required skill

and patience. ▪ Surveying the Internet for specimen sizes was challenging. ▪ Gametogenesis tissues and cells are presented in Table 1.

o Male and Female structures ▪ Sample measurements from the FOV Calculator are presented in Table 2. ▪ Table 3 lists the size measurements for the cells and structures of Gametogenesis ▪ Figure 1 compares the cells and structure sizes of Gametogenesis

Analysis / Discussion:

▪ We observed that the reproductive organ cells have very different sizes. The female ovary and oocyte cells are comparably larger than the male sperm and spermatogonia.

▪ All the cells share some resemblance in shape and form except for the sperm. ▪ The testis and ovary have similar form and characteristics in the images examined whereas the

oocytes and mature follicles have similar shapes and nuclei. Conclusions/Further Considerations:

▪ Even though all the cells look like they could potentially be around the same size, by using the calculations- we are able to see in Fig 1 that they are very different sizes.

▪ I learned that the oocyte is 20x larger then the sperm cell. This makes sense since the oocyte has the opportunity to develop into a mature egg.

▪ I found it interesting that the ovary and check cell were quite similar in size. ▪ I think it takes a very meticulous and patient person to be able to study and analyze cells and life

at the second to smallest level on a daily basis. References

1. http://medical-dictionary.thefreedictionary.com/graafian+follicle

2. http://www.3dscience.com/Resources/Reproductive_Cells.php

3. http://www.gettyimages.com/detail/photo/shows-seminiferous-tubules-spermatogenesis-high-res-stock-photography/139803567

4. http://www.montgomerycollege.edu/~wolexik/205_histology__page.htm


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ATTACHMENTS:

Table 1: Illustrations/Notes of cells- (Human Gametogenesis, Testis, Sperm, and Ovary

Table 1 HumanGametogenesis

Specimen Key Events Notes / Description /Illustration Low MagnificationPhoto

Medium MagnificationPhoto

High MagnificationPhoto

Very High MagnificationPhoto

Testis Sperm Production in the Seminiferous Tubules (which contain the nurse cells)

Web Reference pg 642 http://www.lab.anhb.uwa.edu.au/mb140/corepages/malerepro/Images/tey001he.jpg

http://www.workshopplus.com/ProductCart/pc/catalog/testes_493_detail.jpg

http://www.microscopy-uk.org.uk/mag/imgjan14/dwIMG_1226le

http://www.toxicologyinternational.com/articles/2012/19/2/images/STOX_2012_19_2_182_97220_u3.j

Cell Size µM LM X 75 LM 120 (bar is 100 microns)

Sperm

Mitosis of spermatogonium Meiosis I Meiosis II

Spermiogenesis

Web Referencehttpreproductionandbreeding.weebly.comuploads1

831183137971531373_orig.jpghttp://ww1.prweb.com/prfiles/2011/04/07/9229335/

sperm2.jpghttp://bigthink.com/ideafeed/how-sequencing-

sperm-could-help-fight-cancerhttp://i.dailymail.co.uk/i/pix/2010/10/08/article-

1318795-0B875F42000005DC-234_634x436.jpghttp://infinityhousemagazine.com/wp-

content/uploads/2014/12/Screen-Shot-2014-12-12-

Cell Size 5.6 µM

Ovary

Production of Female gametes (ova) Secretion of female sex hormones

(estrogens and progestins, Secretion of inhibin

Web Referencehttp://humanorgans.org/wp-

content/uploads/2014/05/ovaries.jpghttp://medsci.indiana.edu/a215/virtualscope/image

s/ovary_vd1.jpghttp://medsci.indiana.edu/a215/virtualsc

ope/docs/chap11_3.htmhttp://humrep.oxfordjournals.org/content

/26/3/594/F2.large.jpghttp://www.bu.edu/histology/i/14806hoa.

jpgCell Size 55.02 µM 12 cells AX 50µM

Oocytes Undergo meiosis to meiosis I, At puberty they complete meiosis.

Web Referencehttp://www.repropedia.org/sites/repropedia/files/oo

cyte-final.jpghttp://legacy.owensboro.kctcs.edu/GCaplan/anat2/

notes/RE006b.jpghttp://www.firstscience.com/home/images/stories/e

gg.jpghttp://aquaticpath.phhp.ufl.edu/fhm/images/reprod.

JPGhttp://aquaticpath.phhp.ufl.edu/fhm/images/reprod.

JPG

Cell Size 100 µM

Mature Follicle

Primordial Follicles, Formation of Primary Follicle, Formation of

Secondary Follicle, Formation of Tertiary Follicle, Formation of Corpus

Luteum, Ovulation

Web Referencehttp://faculty.southwest.tn.edu/rburkett/repro_15.jp

ghttp://www.intechopen.com/source/html/26745/me

dia/image2.jpeghttp://web.gccaz.edu/~rbowker/RESPEXCRREPR

OHISTO/MatureFollicle.jpghttp://starklab.slu.edu/Physio/Cumulativ

ePhyLect.htmhttp://www.montgomerycollege.edu/faculty/~wolexi

k/public_html/Ovary-Mature%20Follicle-100x-

Cell Size 5.8 µM


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Table 2 Cell Size Calculator Example measurements


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Table 3 Cell Sizes

Specimen Average Size mm Average Size µm

Sperm 0.01 5.6

Spermatogonia 0.02 17.50

Oocyte 0.10 100.00

Ovary 0.06 55.02

Cheek Cell 0.04 43.75


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Figure 1 Cell Sizes

lab report1 gametogenesis a karle150211 - napa valley...

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