Lab Manual: FTT 3163

FTT 3163 Pengeluaran Ternakan

Name of the Expt: Estimation of GI Nematode Eggs Per Gram Feces

(EPG)

Object!e": To #etermne the En#o Para"tc Loa# n rumnant" from Fecal Egg

$ount

Materal" %e&ure#

1' McMa"ter countng "l#e(' )e!e"3' *ra#uate# Ppette"+' Pa"teur ppette,' )patula"6' )aturate# Na$l "oluton-' .eghng balance/' $ompoun# Mcro"cope

Proce#ure

1' )u"pen# ,g accuratel0 eghe# fre"h fece" n +, ml "aturate# "o#um

chlor#e "oluton 2rato 1:4' Therefore5 #luton factor 17'(' )tran the "u"pen"on through a 8ne "e!e' %emo!e the larger partcle" of

fece" an# let the re"#ue pa"" out 2r"k " there to reman lot of egg" n the

re"#ue"4'3' )tr the "u"pen"on ell n or#er to obtan a completel0 homogenou"

#"trbuton of the egg" n the l&u#'+' 9"ng a pa"teur ppette5 8ll one compartment of the McMa"ter "l#e at

once5 tltng th" a lttle to let all arbubble" e"cape' %epeat the "ame

operaton to 8ll the "econ# countng chamber',' ;llo fe mnute" to <oat up the egg" of the concentraton "oluton an#

"tck to the co!er gla""'6' Make a count of egg" n ether compartment un#er lo magn8caton of

mcro"cope'

Each compartment ha" a "urface of 17 x 17 "& mm' )pace beteen object

gla"" an# co!er "lp " 1', mm' =men"on of each compartment

217x17x1',4>1777 cucm 7'1, cc' Each compartment contan" 7'1, ml

l&u#', g of fece" " #""ol!e# n +, ml of "aturate# Na$l "oluton' )o #luton

rato 1:17

 

)o5  EPG=

 No of eggs∈two chambers

 Area of two chambers  xdilutionfactor

)uppo"e No of egg" counte# n to chamber" ?

EP* Y 

0.15+0.15 x10

 

Haemonchus

McMa"ter

 

 

Lmtaton" an# nterpretaton" of Egg $ount"@t " mpo""ble to calculate from the EP* the prec"e "Ae of the orm

populaton n the ho"t' Too man0 factor" n<uennce both the egg

pro#ucton an# number of egg" foun# per gram of fece"'

1' Be"#e" eggla0ng female orm"5 a number of male an# e"pecall0

lar!al orm" occur5 hch can not be #emon"trate# b0 mean" of the

EP*'(' The no' of egg" la# !are" accor#ng to t0pe of orm' $ertan orm

"pece" la0 man0 egg" 2;"car#"5 mo"t $oopera "pp4 an# other" fe

egg" 2Fa"cola5 C0o"trong0lu"4'3' @n a prmar0 nfecton5 no orm egg" are foun# #urng the prepatent

pero#' The egg la0ng begn" th the patent pero# an# #ecrea"e"

a" the orm gro" ol#er'

Nemato#e

 

+' One of the mmune re"pon"e" of the ho"t " the nhbton of the

female orm"D egg pro#ucton5 "o that the no' of egg" la# b0 each

orm ll #ecrea"e n n!er"e proporton to the anmalD" re""tance'

)ometme" egg pro#ucton "top" completel0' Therefore5 ol#er

anmal" often pre"ent a loer EP* than 0ounger one"5 hch ha!e

the "ame no' of orm"',' The egg pro#ucton of mo"t orm" #oe" not "eem to occur

contnuou"l05 but at regular nter!al" 2c0clc4' @n nter tme egg

pro#ucton " much loer than n the "prng' )ome orm" la0 n the

earl0 afternoon5 other" n the mornng an# n the e!enng' @n or#er

to obtan a !er0 prec"e egg count5 fece" of a (+hour #a0 "houl# be

examne#' =a0 to #a0 !araton n egg pro#ucton " al"o e!#ent'6' The con""tenc0 of the fece" ma0 aect the EP* marke#l0' The more

ater0 the fece" are5 the more the egg" are #lute#'-' ;nmalD" nutrtonal con#ton an# the u"e of certan anthelmntc"

n<uence the egg pro#ucton'/' =e"pte all the"e factor"5 the fece" examnaton an# the egg count

are "tll of !alue n the nterpretaton of the clncal n!e"tgaton'' The EP* of ce"to#e" nfecton" ha" onl0 a #agno"tc !alue'

*ol#en %ule"

1' ;la0" perform the examnaton n the "ame con#ton" an# appl0 the

"ame metho#5 "o that comparable re"ult" ll be obtane#'(' Onl0 examn: 4 Fre"h fece" taken cleanl0 4 "ample "houl# be

repre"entat!e5 large enough an# polle#'3' Examne each preparaton completel0 an# "0"tematcall0 un#er lo

magn8caton'