Lab Exercise 6a-2 - North Seattle 6a-2... · 2007-10-11 · Lab Exercise 6a-2 Connective Tissue Nervous…

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1Lab Exercise 6a-2Connective TissueNervousMuscleClassification of connective tissuesConnective TissueConnective tissue proper Fluid connective tissue Supportive connecting tissueConnective tissuesConnective tissue properLoose connective tissue AreolarAdiposeReticularDense connective tissueDense regularDense irregularElastic tissueFluid CTBloodSupporting CTsCartilageHyaline cartilageElastic cartilageFibrocartilage BoneAreolar tissueA loose CTPAreolar2Areolar: what to look forFibroblastsCollagen fibersElastic fibersMast cells and macrophagesFound? Throughout body, under dermis, divides skin from underlying tissuesFibroblastsResting fibroblasts typically have so little cytoplasm that the cells appear, by light microscopy, as "naked" nuclei Fibroblast Adipose tissue Another lose CTP (note nucleus)Adipose: what to look forLots of cytoplasmSlim nuclei pushed off the sideFound? You know whereReticular tissueThe third type of loose CTP3Reticular tissue Reticular: what to look for Reticular fibers (network)Found? Internal framework in many sort organs (liver, spleen) supporting the parenchymaDense CTPDense regular strength in one directionDense irregular strength in all directionsElastic tissue - pliableDense regularDense regular: what to look forThick parallel bundles of collagenSmall fibroblasts in between bundlesFound? Tendons, ligaments, deep fascia. Dense irregular 4More dense irregularDense irregular: what to look forMesh of collagen fibers (irregular looking)Interspersed fibroblastsFound? Dermis of skin, periosteum, perichondriumElastic tissue Elastic tissue: what to look forElastic fibers (instead of collagen fibers) in large bundlesFibroblastsFound? Between vertebrae, in blood vessel walls (underneath endothelium)Fluid CTBloodBlood: what to look forRBCsWhite blood cells (darker): monocytes, lymphocytes, granulocytesPlatelets5Supportive CTCartilage gelatinous, paddingHyaline cartilageElastic cartilageFibrocartilageHyaline cartilageGlasslike because fibers not visibleMore hyalineThere are collagenous and elastic fibers lying in the cartilage matrix but they are invisible because their refractive index is the same as that of the matrix (like cornea)More hyalineHyaline cartilage HyalineHyaline cartilage (lavender matrix), with perichondrium (pink) outside it. The latter is a dense regular collagenous CT. Cartilage cells = chondrocytes, and they are lying in the lacunae. 6Hyaline cart.: what to look forChondrocytes and lacunaeNo visible fibersWhere? Most joints, nasal septumElastic cartilageElastic Cartilage Elastic cart: what to look forMany elastic fibers in matrixChondrocytes in lacunaeMay be stacked upFibrocartilage Fibrocartilage: what to look forIrregular, wispy collagen fibersChondrocytesFound? Intervertabral discs of spine, pads in knee joint7Supportive CT: BoneDetail of lacuna, showing radiating canaliculi. Tissue fluid from the capillaries and connective tissue of the Haversian canal can seep through these spaces and channels, bringing nutrients to the stellate osteocytes residing there. Bone: what to look forOsteon (whole circular structure)Concentric lamellae (of matrix)Central canal (at center of lamellae)OsteoblastsOsteocytes in lacunaeCanaliculiFound? Bones!Nervous tissueNeuron smearLarge, pyramidal cell bodiesLong processes extending outNervous TissueFigure 4.103 Types of Muscle TissueSkeletal muscle:large body muscles responsible for movementCardiac muscle:found only in the heartSmooth muscle:found in walls of hollow, contracting organs (blood vessels; urinary bladder; respiratory, digestive and reproductive tracts)8Muscle Tissue: SkeletalLong, cylindrical, multinucleate cells with obvious striationsFound in skeletal muscles that attach to bones or skinMuscle Tissue: SkeletalFigure 4.11aMuscle Tissue: CardiacBranching, striated, uninucleate cells interlocking at intercalated discsMuscle Tissue: CardiacFigure 4.11bMuscle Tissue: SmoothSheets of spindle-shaped cells with central nuclei that have no striationsFound in the walls of hollow organsMuscle Tissue: SmoothFigure 4.11c9ExercisesLook at all slidesDraw an example of each tissue on paper provided11 connective tissues:6 CTP (3 loose, 3 dense)1 Fluid CT (blood) 4 Supportive CT (3 cartilage, 1 bone)Neurons3 Muscle tissuesSkeletalStriatedSmoothTurn in on Thurs 10/257 drawings from 6a-1 Epithelia15 Drawings from 6a-2 Connective+Review sheet for lab 6a