Lab Exercise 6a-2 - North Seattle 6a-2... · 2007-10-11 · Lab Exercise 6a-2 Connective Tissue Nervous…

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    Lab Exercise 6a-2

    Connective TissueNervousMuscle

    Classification of connective tissues

    Connective Tissue

    Connective tissue proper Fluid connective tissue Supportive connecting tissue

    Connective tissuesConnective tissue proper

    Loose connective tissue AreolarAdiposeReticular

    Dense connective tissueDense regularDense irregularElastic tissue

    Fluid CTBlood

    Supporting CTsCartilageHyaline cartilageElastic cartilageFibrocartilage


    Areolar tissue

    A loose CTP


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    Areolar: what to look for

    FibroblastsCollagen fibersElastic fibersMast cells and macrophages

    Found? Throughout body, under dermis, divides skin from underlying tissues


    Resting fibroblasts typically have so little cytoplasm that the cells appear, by light microscopy, as "naked" nuclei

    Fibroblast Adipose tissue

    Another lose CTP (note nucleus)

    Adipose: what to look for

    Lots of cytoplasmSlim nuclei pushed off the side

    Found? You know where

    Reticular tissue

    The third type of loose CTP

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    Reticular tissue Reticular: what to look for

    Reticular fibers (network)

    Found? Internal framework in many sort organs (liver, spleen) supporting the parenchyma

    Dense CTP

    Dense regular strength in one directionDense irregular strength in all directionsElastic tissue - pliable

    Dense regular

    Dense regular: what to look for

    Thick parallel bundles of collagenSmall fibroblasts in between bundles

    Found? Tendons, ligaments, deep fascia.

    Dense irregular

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    More dense irregularDense irregular: what to look for

    Mesh of collagen fibers (irregular looking)Interspersed fibroblasts

    Found? Dermis of skin, periosteum, perichondrium

    Elastic tissue Elastic tissue: what to look for

    Elastic fibers (instead of collagen fibers) in large bundlesFibroblasts

    Found? Between vertebrae, in blood vessel walls (underneath endothelium)

    Fluid CT


    Blood: what to look for

    RBCsWhite blood cells (darker): monocytes, lymphocytes, granulocytesPlatelets

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    Supportive CT

    Cartilage gelatinous, paddingHyaline cartilageElastic cartilageFibrocartilage

    Hyaline cartilage

    Glasslike because fibers not visible

    More hyaline

    There are collagenous and elastic fibers lying in the cartilage matrix but they are invisible because their refractive index is the same as that of the matrix (like cornea)

    More hyaline

    Hyaline cartilage Hyaline

    Hyaline cartilage (lavender matrix), with perichondrium (pink) outside it. The latter is a dense regular collagenous CT. Cartilage cells = chondrocytes, and they are lying in the lacunae.

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    Hyaline cart.: what to look for

    Chondrocytes and lacunaeNo visible fibersWhere? Most joints, nasal septum

    Elastic cartilage

    Elastic Cartilage Elastic cart: what to look for

    Many elastic fibers in matrixChondrocytes in lacunaeMay be stacked up

    Fibrocartilage Fibrocartilage: what to look for

    Irregular, wispy collagen fibersChondrocytes

    Found? Intervertabral discs of spine, pads in knee joint

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    Supportive CT: BoneDetail of lacuna, showing radiating canaliculi. Tissue fluid from the capillaries and connective tissue of the Haversian canal can seep through these spaces and channels, bringing nutrients to the stellate osteocytes residing there.

    Bone: what to look forOsteon (whole circular structure)Concentric lamellae (of matrix)Central canal (at center of lamellae)OsteoblastsOsteocytes in lacunaeCanaliculi

    Found? Bones!

    Nervous tissue

    Neuron smearLarge, pyramidal cell bodiesLong processes extending out

    Nervous Tissue

    Figure 4.10

    3 Types of Muscle Tissue

    Skeletal muscle:large body muscles responsible for movement

    Cardiac muscle:found only in the heart

    Smooth muscle:found in walls of hollow, contracting organs (blood vessels; urinary bladder; respiratory, digestive and reproductive tracts)

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    Muscle Tissue: Skeletal

    Long, cylindrical, multinucleate cells with obvious striationsFound in skeletal muscles that attach to bones or skin

    Muscle Tissue: Skeletal

    Figure 4.11a

    Muscle Tissue: Cardiac

    Branching, striated, uninucleate cells interlocking at intercalated discs

    Muscle Tissue: Cardiac

    Figure 4.11b

    Muscle Tissue: Smooth

    Sheets of spindle-shaped cells with central nuclei that have no striationsFound in the walls of hollow organs

    Muscle Tissue: Smooth

    Figure 4.11c

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    ExercisesLook at all slidesDraw an example of each tissue on paper provided11 connective tissues:

    6 CTP (3 loose, 3 dense)1 Fluid CT (blood) 4 Supportive CT (3 cartilage, 1 bone)

    Neurons3 Muscle tissuesSkeletalStriatedSmooth

    Turn in on Thurs 10/25

    7 drawings from 6a-1 Epithelia15 Drawings from 6a-2 Connective+Review sheet for lab 6a