june 27, 2006ceo networking luncheon1 notice!!! prices are subject to change

June 27, 2006June 27, 2006 CEO Networking LuncheonCEO Networking Luncheon 11

NOTICE!!!

PRICES ARE SUBJECT TO

CHANGE.


CHEMISTRY SERVICES at UMBCCHEMISTRY SERVICES at UMBC

by

WILLIAM R. LaCOURSE

Department of Chemistry and BiochemistryUniversity of Maryland, Baltimore County

1000 Hilltop CircleBaltimore, MD 21250

PH: 410-455-2105 FX: 410-455-2608Email: [email protected]


GUIDE TO SHOPS AND SERVICES

Mass Spectrometry Facility

Nuclear Magnetic Resonance Facility

Other Instrumentation

Shops – Glass, Electronic & Machine

Contract Research

Consultancy


OVERVIEW All instrumentation and shops

owned and operated by the Dept. of Chemistry & Biochemistry

UMBC researchers/outside customers - charged fees for use Fees – pay costs of operation (NOT FOR PROFIT) Costs

• General housing, utilities, insurance, service contracts, maintenance and repairs

• Specific examples MS: gases, pumps, software, and supplies NMR: cryogens, software, and supplies

Technicians - ‘soft’ money positions Maintain and operate instrumentation and shops Salary support

• Cost of service – included in fees• Contract research• Contract teaching• Limited departmental subsidy


MASS SPECTROMETRY (MS) FACILITY

PURPOSE a resource offering service, training and instrumentation with

the primary aim of providing investigators with access to contemporary mass spectrometry instrumentation and expertise.

Biomolecule Applications Protein Chemistry

• Protein ID by peptide mass fingerprinting using MALDI • Determination of protein MW • Sample composition validation • Protein purification assessment

Oligonucleotide Analysis• Identity and purity validation

Small Molecules Applications• Structure elucidation by tandem mass spectrometry (MSn) • Elemental composition determination through accurate mass

measurement General Analytical Services

• LC-MS and GC-MS purity assessment • Impurities and degradants profiling


MS INSTRUMENTATION Bruker AUTOFLEX (2002) MALDI Time of flight

Range: Linear: > 250 kDa Reflectron: > 5,000 Da Resolution Linear: > 4,000 (FWHM) Reflectron: >10,000 (FWHM) Characterization of proteins, oligonucleotides and other polymers. Characterization and identification of proteins by peptide mass mapping and

sequencing.

Bruker ESQUIRE 3000 (2002) ESI, APCI, Ion Trap Range: 50-3000 Resolution: Nominal mass measurement capability throughout the entire m/z

range Electrospray ionization (ESI), atmospheric press. chem. ionization (APCI) Analysis of non-volatile and thermally-labile compounds; protein mass

determination and digest analysis; peptide sequencing.

Shimadzu GC-17A/QP-5000 (1993+) EI, Quadrapole Mass Range: 10-700 Analysis of volatile (GC amendable) compounds

JEOL HX110/HX100 (1986) EI, CI, FD, FAB, ESI Sector Range: 1- >10,000 Resolution: Up to 100,000 ESI Applications Mostly used for exact mass measurement using FAB (Fast Atom

Bombardment)


SUBMITTED SAMPLE ANALYSIS

UMBC researchers and outside users can submit samples for the following services:

FAB unit resolution MS FAB accurate mass measurement and

empirical formula determination. MALDI-TOF ESI or APCI direct infusion ESI or APCI LC-MS with HPLC (reverse and

normal phase) separation GC-MS


SAMPLE SUBMISSION Identify the most suitable

approach which will provide information you are looking for

Become familiar with sample preparation guidelines and make sure that the sample(s) meet the guidelines

Fill out a sample submission form

Deliver samples accompanied by the sample submission form(s).

It is suggested that we receive samples as solid material (just enough to see it) or neat liquid (several ul) for FAB, ESI, APCI. For MALDI-TOF analysis submit ~10 pmol of peptide, protein or oligonucleotide (>100 pmol) dry or in minimal (several µl) volume of solvent.


MS FACILITY RATES

MALDITOF: $55/sample or $130/h

ESI or APCI direct infusion $60/sample or 130/h

ESI or APCI LC-MS separation $100/h

GC-MS $80/h

FAB Lo-Res: $55/sample Hi-Res: $90/sample

Time is rounded to the next hour/no method development

Method Development – costs handled on an individual basis.


WORKSHOPS GC Mass Spectrometry (GC-MS) Workshop: 1 day

Topics will include: Instrument overview: Gas chromatograph, Quadrapole MS, ion

detection, vacuum system EI source and spectra interpretation Analysis by EI full scan and selected ion monitoring mode Quantitation of unknown samples Hands-on sample running and covering of applications requests

Fees - $295.00 per participant includes course materials, lab supplies and instrument time. Participants will have ample opportunity to discuss their

applications. Also the attendees will receive guidance on using the facility

instruments and expertise to meet their goals.


WORKSHOPS MALDI Time of Fight Mass Spectrometry (MALDI-TOF) Workshop (2

days)

Participants will: Learn basic theory necessary for intelligent instrument operation. Receive training on instrument operation and data analysis. Prepare and analyze several representative samples. Learn about MALDI-TOF capabilities and limitations due to instrument, sample

preparation and substrate issues.

Workshop schedule: Day 1

• Facility tour and instrument operation demonstration.• Mass spectrometry primer, MALDI overview and basic theory of TOF instrumentation. • Instrument operation overview. Participants prepare samples and acquire data with

assistance as necessary. Data analysis software overview followed by analysis of acquired spectra.

Day 2• First day material review.• A detailed study on the effect of sample concentration and level of impurities on data

quality.

Fees -$495.00 per participant includes course materials, lab supplies and instrument time. Participants will have ample opportunity to discuss their applications. attendees will receive guidance on using the facility instruments and expertise to

meet their goals.


WORKSHOPS Liquid Chromatography-Mass Spectrometry (LC-MS) Workshop (2

days)

Participants will: Learn basic theory governing instrument operation. Receive training on instrument operation and data analysis. Prepare and analyze several representative samples. Learn about LC-MS capabilities and limitations due to instrument, sample

preparation and matrix issues = WISDOM.

Workshop Schedule: Day 1

• Facility tour and instrument operation demonstration.• Mass spectrometry primer, electrospray ionization overview and basic theory of ion traps.• Instrument operation overview. Participants prepare samples and acquire data with

assistance as necessary. Data analysis software overview followed by analysis of acquired spectra.

Day 2• First day material review.• A discussion on HPLC requirements: Column and mobile phase options and an Introduction

to MS/MS.• Setting up a MS/MS experiment followed by data analysis and discussion.

Fees - $495.00 per participant includes course materials, lab supplies and instrument time. Participants will have ample opportunity to discuss their applications. attendees will receive guidance on using the facility instruments and expertise to

meet their goals.


MS FACILITY MANAGER

Dr. Alexei Gapeev

Office: Room 140-A, Meyerhoff Building

Office Phone : 410-455-6251

Fax: 410-455-2608

E-mail address: [email protected]

Initial Contact should be made through Dr. Bill LaCourse.


NMR FACILITY Seven (7) NMR spectrometers

ranging from 800MHz to 200MHz

Howard Hughes Medical Institute – biomolecular studies

Three (3) Bruker NMR spectrometers• one DMX800 equipped with cryoprobe • two DMX600, one equipped with cryoprobe.

Graduate and Undergraduate Research – small molecule focus

Bruker DRX500 with cryoprobe JEOL ECX400 GE QE300

Undergraduate Education - teaching Varian Mercury 200


NMR FACILITY PURPOSE

serve as a resource offering service, training and instrumentation with the primary aim of providing investigators with access to contemporary NMR instrumentation and expertise.

The NMR Facility - commercial analytical services Focus: 1H, 13C, 15N

Rates 500MHz:

• 50$/hour(Commercial) • 100$/hour(Consultation)• 150$/hour(Commercial, Assisted)

400MHz: • 50$/hour(Commercial)• 100$/hour(Consultation) • 150$/hour(Commercial, Assisted)


NMR FACILITY MANAGER

Dr. David Yeh

Office: Room 247B, Meyerhoff Building

Office Phone : 410-455-1031

Fax: 410-455-2608

E-mail address: [email protected]

Initial Contact should be made through Dr. Bill LaCourse.


OTHER INSTRUMENTATION Specialty Instrumentation & Techniques

Examples• X-RAY Diffractometer• EPR Spectrometer• Absorption and Luminescence spectrophotometers• ThermoNicolet FT-IR spectrometer • Circular Dichroism• Calorimetry• Electrochemical Analysis

Operation – University personnel only

Access – may be limited

Fees – individually contracted

Contact – Dr. Bill LaCourse


GLASS SHOP PURPOSE

Custom glassblowing services for the UMBC community and off-campus clients.

DESCRIPTION Expert scientific glassware and glass component repair,

design assistance and manufacture of novel glass pieces, and general glassblowing services.

RATES $35.00/hour plus the cost of materials.

CONTACT Mr. Anthony Baney, Glass Artisan Initial Contact – Dr. Bill LaCourse


ELECTRONIC & MACHINE SHOPS

PURPOSE Electronic repair and custom machining services for the

UMBC community and off-campus clients

RATES $35.00/hour plus the cost of materials.

CONTACTS Electronic services

• Mr. Nikollai Galitsky, Electronic Technician• Initial Contact – Dr. Bill LaCourse

Machine shop services• Mr. John Cataldi, Machinist• Initial Contact – Dr. Bill LaCourse


CONSULTANSHIP/CONTRACT RESEARCH Faculty - a group of faculty members who are leaders in scientific

research. Their activity is reflected in the amount of outside funding for research in the

department, currently over $4 million per year.

Center for Structural Biology

Howard Hughes Medical Institute

Newly Renovated Building Modern conference and seminar rooms.

Extensive array of tools for modern chemical and biochemical research Calorimetry, chromatography, stopped-flow and temperature-jump kinetics,

transient laser spectroscopy, NMRs, X-band CW electron paramagnetic resonance spectrometry, circular dichroism, X-ray diffraction, infrared spectroscopy, atomic absorption- and gas chromatography-mass spectrometry, and Fourier transform ion cyclotron resonance mass spectrometry apparatus, as well as extensive molecular modeling and computational chemistry facilities.

Ready access to literature Department reference room Albin O. Kuhn library - 2,500 volumes of text/> 150 chemistry and biochemistry

periodicals on-site Modern e-journal browsing


CONTACT INFORMATION

Visit our website www.umbc.edu/chem-biochem/

Shops.html

Head of Business Operations Dr. Bill LaCourse

Utilize departmental resources to support research, economic viability, and outreach.

june 27, 2006ceo networking luncheon1 notice!!! prices are subject to change

Documents

mass range

exact mass measurement

peptide mass fingerprinting

peptide mass mapping

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chemistry services

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instrumentation shops