j:!quality!document revisions!!aledgppng-lbtdw-gppng-lb … · 2019-08-04 · 1 glykoprep ®-plus...

42
1 GlykoPrep ® -plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology +X K_^YWK^ON RSQRVc \YL_]^ RSQR^R\Y_QRZ_^ Z\YMO]] PY\ OXdcWK^SM NOQVcMY]cVK^SYX PV_Y\O]MOX^ VKLOVSXQ aS^R 3X]^KX^+, KXN MVOKX_Z YP ObMO]] NcO PY\ KXKVc]S] Lc 6- KXN Y^RO\ WO^RYN] & 8YX]OVOM^S`O \KZSN \OVOK]O KXN \OMY`O\c YP 8QVcMKX] P\YW _Z ^Y # QVcMYZ\Y^OSX ]KWZVO] K^ K ^SWO YX ^RO +QSVOX^ +]]Kc7+: ,\K`Y 6S[_SN 2KXNVSXQ :VK^PY\W +]]Kc7+: ,\K`Y & 7SXSWKV RKXN]YX ^SWO & 9Z^SWSdON \OKM^SYX MYXNS^SYX] ROVZ ^Y Z\O]O\`O VKLSVO QVcMKX] ]_MR K] ]SKVSM KMSN & 8YX]OVOM^S`O MROWS]^\c PY\ ]^YSMRSYWO^\SM VKLOVSXQ YP QVcMKX] SXNOZOXNOX^ YP ]^\_M^_\O & <> 3X]^KX^+, VKLOVSXQ aS^R XY VY]] YP ]SKVSM KMSN% VKLOVSXQ OPPSMSOXMc ( " & :_\SPSON 3X]^KX^+,VKLOVON 8QVcMKX] K\O OV_^ON SX aK^O\ \OKNc PY\ KXKVc]S] :\YN_M^ -YNO$ 1::816, NOTICE: ProZyme was purchased by Agilent in July 2018. Documents for products and product lots manufactured before August 2019 will contain references to ProZyme. For more information about these products and support, go to: www.agilent.com/en/contact-us.

Upload: others

Post on 20-Jul-2020

0 views

Category:

Documents


0 download

TRANSCRIPT

Page 1: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

1

Gly

koP

rep®

-plu

s R

apid

N-G

lyca

n S

ampl

e P

repa

ratio

n w

ith In

stan

tAB

Aut

omat

ed o

n A

ssay

MA

P T

echn

olog

y

NOT

ICE:

Pro

Zym

e w

as p

urch

ased

by

Agile

nt in

Ju

ly 2

018.

Doc

umen

ts fo

r pro

duct

s an

d pr

oduc

t lo

ts m

anuf

actu

red

befo

re A

ugus

t 201

9 w

ill

cont

ain

refe

renc

es to

Pro

Zym

e. F

or m

ore

info

rmat

ion

abou

t the

se p

rodu

cts

and

supp

ort,

go to

: www.agilent

.com

/en/

cont

act-us

.

Page 2: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

2

TA

BL

E O

F C

ON

TE

NT

S

Page 3: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

3

EQ

UIP

ME

NT

SO

FT

WA

RE

AN

D M

AN

UA

LS

HO

W T

O U

SE

TH

IS B

OO

KL

ET

Page 4: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

4

KIT

CO

NT

EN

TS

Sto

rag

e R

equ

irem

ents

Page 5: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

5

Ad

dit

ion

al R

equ

ired

Rea

gen

ts/E

qu

ipm

ent

Op

tio

nal

Rea

gen

ts a

nd

Su

pp

lies

SA

FE

TY

AN

D H

AN

DL

ING

Page 6: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

6

INT

RO

DU

CT

ION

US

ING

TH

E K

IT

Page 7: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

7

Gly

cop

rote

in S

amp

le P

rep

arat

ion

Page 8: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

8

Page 9: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

9

Page 10: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

10

Usi

ng

th

e R

eag

ent

Vo

lum

e C

alcu

lato

r

Page 11: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

11

Gly

koP

rep

-plu

s R

eag

ent

Pre

par

atio

n

Page 12: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

12

Ass

ayM

AP

Bra

vo P

roce

ssin

g T

ime

Page 13: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

13

Pre

par

ing

fo

r th

e G

lyko

Pre

p-p

lus

Pro

toco

ls

Page 14: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

14

Page 15: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

15

Page 16: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

16

GL

YK

OP

RE

P-P

LU

S P

RO

TO

CO

LS

Page 17: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

17

1P

late

& R

eag

ent

Set

up

Pro

toco

l

Page 18: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

18

Page 19: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

19

2R

X C

artr

idg

e S

etu

p P

roto

col

Page 20: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

20

Page 21: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

21

3Im

mo

bili

zati

on

, Dig

esti

on

& In

stan

tAB

Lab

elin

g P

roto

col

Page 22: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

22

Page 23: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

23

Page 24: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

24

4C

U C

artr

idg

e S

etu

p P

roto

col

Page 25: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

25

Page 26: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

26

5C

lean

up

Pro

toco

l

Page 27: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

27

Page 28: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

28

Page 29: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

29

QUICKGUIDE

Glycoprotein Sample Plate Preparation

Page 30: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

30

Page 31: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

31

1 Plate & Reagent Setup Protocol

Page 32: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

32

2 RX Cartridge Setup Protocol

Page 33: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

33

3 Immobilization, Digestion & InstantAB Labeling Protocol

Page 34: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

34

Page 35: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

35

4 CU Cartridge Setup Protocol

Page 36: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

36

5 Cleanup Protocol

Page 37: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

37

Page 38: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

38

ANALYSIS OF LABELED N-GLYCANS

TIPS & HINTS

Page 39: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

39

REFERENCES

TECHNICAL ASSISTANCE

TOLL FREE (800) 457-9444 (US & CANADA)

PHONE (510) 638-6900FAX (510) 638-6919

E-MAIL [email protected] www.prozyme.com

Page 40: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

40

LICENSE TO USE

TRADEMARKS AND TRADENAMES

PRODUCT USE AND WARRANTY

OTHER PROZYME PRODUCTS & KITS

Page 41: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology

41

ORDERING INFORMATION

3832 Bay Center PlaceHayward, CA 94545-3619

Page 42: J:!Quality!Document Revisions!!AledGPPNG-LBTDW-GPPNG-LB … · 2019-08-04 · 1 GlykoPrep ®-plus Rapid N-Glycan Sample Preparation with InstantAB Automated on AssayMAP Technology