Journal ofClinical Chemistry andClinical Biochemistry

Zeitschrift fürKlinische Chemie undKlinische BiochemieGemeinsames Organ der Deutschen, der Niederländischen, der Österreichischen undder Schweizerischen Gesellschaft für Klinische Chemie

Editors in Chief Verantwortliche Herausgeber

Johannes Büttner, Hannover Ernst Schütte, Berlin

Managing Editor Schriftleiter Friedrich Körber, BerlinSpecial Editor for IFCC Recommendations Nils-Erik Särrs, Helsinki

Editors Herausgeber

Hugo Aebi, BernHeinz Breuer, BonnJohannes Büttner, HannoverHans Joachim Dulce, BerlinJörg Frei, LausanneWolfgang Gerok, FreiburgHelmut Greiling, Aachen

Advisory Board unter Mitarbeit von

Klaus Borner, BerlinEckhart Buddecke, MünsterHans-Christoph Curtius, ZürichManfred Doss, MarburgHartmut Dost, GießenHans Faillard, SaarbrückenGünther Fuchs, BerlinErich Gladtke, KölnHeinz-Werner Goedde, HamburgErwin Hansert, München

Erich Kaiser, WienEsso Johannes van Kämpen, GroningenHermann Mattenheimer, ChicagoErnst Schütte, BerlinDankwart Stamm, MünchenHansjürgen Staudinger, FreiburgOtto Wieland, München

Hans Ludwig Krüskemper, DüsseldorfGeorg Löffler, RegensburgMathias M. Müller, WienKurt Oette, KölnJean-Paul Persijn, AmsterdamLadislaus Röka, GießenEllen Schmidt, HannoverIvar Trautschold, HannoverGerhard Uhlenbruck, Köln

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GWalter de Gruyter • Berlin • New YorkJ. Clin. Chem. Clin. Biochem. Volume 19, Number 8, August 1981, pages 513-900

602 Abstracts: XI. International / IV. European Congress of Clinical Chemistry, Vienna

Arnstadt, K.-L and D. Berg

Sensitive Enzyme-Immunoassay for Progesterone inHuman Plasma and Saliva

Institut für Physiologie der Südd. Versuchs- und For-schungsanstalt für Milchwirtschaft, Techn. UniversitätMünchen, Freising- Weihenstephan

High sensitivity enzyme-immunoassays (EIA) forsteroid hormones are achieved by the use ofheterologous systems, sophisticated purification ofthe enzyme-coupled hormone by affinity chromato-graphy (1) and a new type of methodology, thefluoro-enzyme-immunoassay (2). A /?£^rologousEIA for progesterone covering the range of0.1-10 pmol/test tube (0.03-3 ng) (3) was improvedto measure high sensitivities of 10-1000 fmol (3—300 pg) by simply changing the test conditions butstill using a normal photometer. Results with thisEIA are shown for the measurement of physiologicallevels or in follow-up therapeutic treatment:

a) Sensitive range (10—1000 fmol) for determinationin human plasma and in saliva. Control of treatmentof infertility. The level in saliva is only about 1/100compared to plasma.

b) normal range (0.1 —lOpmol) for determinationof progesterone in pregnancy. This also seems to be

J. Clin. Chem. Clin. Biochem. / Vol. 19, 1981 / No. 8

Abstracts: XI. International/IV. European Congress of Clinical Chemistry, Vienna 603

possible by direct determination in serum withoutextraction of the progesterone.

The values of direct assay agree well with reportedhormonal levels, for example:

7. week 76 nmol/1 (24 jig/1);10. week 105 nmol/1 (33 fig/1);17. week 150 nmol/1 (47 fig/1);29. week 225 nmol/1 (70 ng/1);38. week 550 nmol/1 (170 ̂ ig/1).

References

1. Abuknesha, R. A. & Exley, D. (1980), J. Clin. Chem. din.Biochem. 18, 662-664.

2. Adlercreutz, H., Lindholm, A.-M., Karonen, S.-L. & Här-könen, M. (1980), J. Clin. Chem. Clin. Biochem. 18, 661-662.

3. Arnstadt, K.-I. & Cleere, W. F. (1981) J. Reprod. Pert. 62,in press.

Dr. Klaus-Ingo ArnstadtInstitut für PhysiologieTU München-WeihenstephanD-8050 Freising

J. Clin. Chem. Clin. Biochem. / Vol. 19, 1981 / No. 8