Jeffrey pine trees killed by the Jeffrey Pine Beetle – Little Valley, Near Reno, NV 1998

Mark McGregor, USDA Forest Service

Pitch tubes:Jeffrey pine beetle attackingJeffrey pine tree

Pine engraver beetle, Ips piniColonizing Jeffrey pine log

JH treated male D. jeffreyiantisense probe to HMG-R

crop Ventriculus (midgut)

rectum

Hall

Pheromone production occursIn midgut of bark beetles

Pheromone-Biosynthetic Gene Expression Profiles:

Induced by feeding or JH treatment

Males-7-6-5-4-3-2-10123456

0 5 10 15 20 25 30 35

Time (h)

Thiolase HMG-SHMG-R DecarboxylaseIsomerase GPPSFPPS

-2

-1

0

1

2

3

4

5

6

7

8

9

10

AACT

HM

G-S

HM

G-R

MK

MPD

C

IPPI

GPP

S

FPPS

log

2(R

elati

ve E

xpre

ssio

n) (+

SE)Basal expression levels

higher in males than females

Localized to anterior midgut