jeff elhai
DESCRIPTION
α -ketoglutarate signals N-deprivation in an association between Nostoc punctiforme and Anthocerus punctatus. Jeff Elhai. Introduction. Background. - PowerPoint PPT PresentationTRANSCRIPT
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α-ketoglutarate signals N-deprivation in an
association between Nostoc punctiforme and Anthocerus
punctatus
Jeff Elhai
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IntroductionBackgroundThe world population is the total number of living humans on Earth. As of today, it is estimated to number 7.055 billion by the United States Census Bureau (USCB).[1] The USCB estimates that the world population exceeded 7 billion on March 12, 2012.[2]
Green revolution plants are able to make use of more N fertilizer.
With advances in molecular genetics, the mutant genes responsible for Arabidopsis thaliana genes (GA 20-oxidase,[17] ga1,[18] ga1-3[19]), wheat reduced-height genes (Rht)[20] and a rice semidwarf gene (sd1)[21] were cloned.
Norman Borlaug is considered the Father of the Green Revolution.
To feed an evergrowing population, it is necessary to increase the availability of food. Green Revolution refers to a series of research, development, and technology transfer initiatives, occurring between the 1940s and the late 1970s, that increased agriculture production around the world, beginning most markedly in the late 1960s.
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IntroductionAdditional Background
Nostoc punctiforme forms symbioses with many plants. It is a heterocystous cyanobacterium that fixes nitrogen in semiregularly spaced cells.
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An experiment
Nostoc passes fixed nitrogen to the plant
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Materials and Methods
GltI was amplified from the genomic DNA by PCR and subcloned into pRSETB (Invitrogen). Truncation combinations were amplified with Phusion polymerase(NEB) and purified with 96-well PCR cleanup cartridges (Qiagen).
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Results
These are the kind of results I expect to get.
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More results
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
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Discussion
World hunger is one of the gravest threats facing our world today. Every year 15 million children die of hunger. If this experiment helps even a small fraction of them it will be worth it.
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References
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Any Questions?