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Visit thermoscientific.com/insider1 January 2 - June 30, 2014 Offers! Great products you depend on. The protein biology resource you trust. Special deals you’ll love. 20% OFF 25% OFF NEW! Get 3 for the price of 2 Get 4 for the price of 3 Protein Transfer Thermo Scientific Pierce G2 Fast Blotter Inhibitor Cocktails Thermo Scientific Halt Protease and Halt Phosphatase Inhibitor Cocktails Magnetic Immunoprecipitations and Co-Immunoprecipitations Thermo Scientific Pierce Magnetic IP/Co-IP Kits Cell Lysis Thermo Scientific Cell Lysis Reagents Western Blot Substrates Thermo Scientific ECL and Thermo Scientific SuperSignal Western Blot Substrates Antibodies eNewsletter Tips, applications and promotions about Thermo Scientific Pierce Antibodies Sign up for 30% OFF

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Visit thermoscientific.com/insider1

January 2 - June 30, 2014 Offers! Great products you depend on. The protein biology resource you trust. Special deals you’ll love.

20%OFF

25%OFF

NEW!

Get 3 for the price

of 2

Get 4 for the price

of 3

Protein TransferThermo Scientific™ Pierce™ G2 Fast Blotter

Inhibitor CocktailsThermo Scientific™ Halt™ Protease and Halt Phosphatase Inhibitor Cocktails

Magnetic Immunoprecipitations and Co-Immunoprecipitations Thermo Scientific™ Pierce™ Magnetic IP/Co-IP Kits

Cell Lysis Thermo Scientific™ Cell Lysis Reagents

Western Blot SubstratesThermo Scientific™ ECL and Thermo Scientific™

SuperSignal™ Western Blot Substrates

Antibodies eNewsletterTips, applications and promotions about Thermo Scientific™ Pierce™ Antibodies

Sign up for 30%OFF

Purchase the new Thermo Scientific Pierce G2 Fast Blotter and receive FREE Thermo Scientific™ Pierce™

1-Step Transfer Buffer, 1L (Product # 84731) and FREE Filter Paper, 7x8.4cm, 100 sheets (Product # 84783).

Pierce G2 Fast Transfer Blotter Highlights:• Fast – transfer proteins in 5-10 minutes

• Excellent transfer efficiency – transfer 10-300kDa proteins equivalent to or better than conventional semi-dry or wet transfer methods

• Integrated power supply – allows consistent high-efficiency protein transfer

• Easy-touch programming – access pre-programmed transfer methods or create your own using color LCD touch screen

• Convenient – simultaneously transfer up to four mini-sized gels or two midi-sized gels

• Versatile – use Pierce 1-Step Transfer Buffer for fast blotting programs or Towbin transfer buffer for conventional semi-dry methods

Pierce 1-Step Transfer Buffer Highlights:• Safe and environmentally friendly – contains

no alcohol

• Economical – transfer up to 20 mini-sized gels or 10 midi-sized gels with one 1L bottle

Fast Transfer Bundle

Protease and Phosphatase Inhibitor Cocktails Get 3 for the price of 2

Stock up on Thermo Scientific Halt Protease and Halt Phosphatase Inhibitor Cocktails at these special savings. Both Halt Phosphatase and Protease Cocktails are supplied as refrigerator-stable (2-8°C) 100X liquid formats.

Phosphatase Cocktail Highlights:• All-in-one format – inhibits both serine-threonine

phosphatases and protein tyrosine phosphatases

• Compatible – works with standard protein assays, including BCA and coomassie (Bradford) assays

Protease Cocktail Highlights:• No proprietary ingredients – fully disclosed formulation

contains AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A in DMSO

• Lysis buffer compatible – use with Thermo Scientific™ Pierce™ Cell Lysis Buffers or nearly any other commercial or homemade detergent-based lysis reagent

Special Offer

NEW!

Get 3 for the

price of 2

Ordering Information

Product # Description Promo Price

62288SPCL Pierce G2 Fast Blotter with 1L Pierce 1-Step Transfer Buffer and 100pk of Filter Paper

* Offer valid January 2, 2014, through June 30, 2014. Must use special Product # listed here to receive this price. Not valid with other offers and discounts.

Ordering Information

Product # DescriptionPkg. Size

Promo Price

78420PROMO Halt Phosphatase Inhibitor CocktailEach 1mL package is sufficient for 100mL of sample

3 x 1mL

87786PROMO Halt Protease Inhibitor CocktailEach 1mL package is sufficient for 100mL of sample

3 x 1mL

* Offer valid January 2, 2014, through June 30, 2014. Must use special Product # listed here to receive this price. Not valid with other offers and discounts.

Highlights:• Optimized – each formulation is

tailored to specific cell type

• Easy-to-use – each product is a single reagent for efficient, one-step lysis

• High yield – better protein yield than home brew or other commercially available formulations

• Convenient – most formulations do not require mechanical lysis

• Compatible – lysates can be used directly in most downstream assays, such as protein assays, SDS-PAGE, Western blotting and immunoassays

Cell Lysis and Protein Isolation Made Easy With Thermo Scientific Cell Lysis Reagents at 25% off, your protein extractions got even easier. These ready-to-use solutions produce protein samples that are compatible with many downstream applications. Choose from some of our more popular formulations at these special prices.

Now’s your opportunity to learn why people are moving to magnetic immunoprecipitations (IPs) and co-immunoprecipitations (co-IPs). Choose from three different kit formats, all at 20% off.

• Thermo Scientific™ Pierce™ Classic Magnetic IP/Co-IP Kit – accomplish high-yield IP or co-IP with manual or automated magnetic-separation tools using less than 10µg of antibody.

• Thermo Scientific™ Pierce™ Crosslink Magnetic IP/Co-IP Kit – uses crosslinking chemistry to covalently immobilize IP antibodies onto high-quality Protein A/G Magnetic Beads for effective IP and co-IP, without interference from antibody fragments.

• Thermo Scientific™ Pierce™ Direct Magnetic IP/Co-IP Kit – uses NHS-chemistry to covalently immobilize IP antibodies onto magnetic beads for effective IP and co-IP, without antibody contamination. Antibodies from any species of subtype can be used.

Thermo Scientific Pierce Magnetic IP/Co-IP Kits Save 20% when you move to magnetic IP/co-IP

25%OFF

20%OFF

Ordering Information

Product # DescriptionPkg. Size Promo Price

88804PROMO Pierce Classic Magnetic IP/Co-IP KitSufficient for 40 IP reactions using 25µL of beads

40 rxn kit

88805PROMO Pierce Crosslink Magnetic IP/Co-IP KitSufficient for 40 IP reactions using 25µL of beads

40 rxn kit

88828PROMO Pierce Direct Magnetic IP/Co-IP KitSufficient for 40 IP reactions using 25µL of beads

40 rxn kit

* Offer valid January 2, 2014, through June 30, 2014. Must use special Product # listed here to receive this price. Not valid with other offers and discounts.

Ordering Information

Product # DescriptionPkg. Size Promo Price

90084PROMO B-PER™ Bacterial Protein Extraction ReagentSufficient for 60g cell paste

250mL

78501PROMO M-PER™ Mammalian Protein Extraction ReagentSufficient for 25g of wet cells (10 million cells per 1mL of reagent)

250mL

78510PROMO T-PER™ Tissue Protein Extraction ReagentSufficient for 25g fresh tissue

500mL

78991PROMO Y-PER™ Yeast Protein Extraction ReagentSufficient for 40 to 80g of wet cells

200mL

89901PROMO RIPA Lysis and Extraction BufferSufficient for approx. 5 million cells per 1mL of reagent

250mL

87788PROMO Pierce™ IP Lysis BufferSufficient for 25,000cm2 of cell monolayer or 25g of cell paste

250mL

87792PROMO N-PER™ Neuronal Protein Extraction ReagentSufficient for 10g tissue or up to 200 x 10cm dishes of primary cultured neurons

100mL

* Offer valid January 2, 2014, through June 30, 2014. Must use special Product # listed here to receive this price. Not valid with other offers and discounts.

thermoscientific.com/pierce© 2013 Thermo Fisher Scientific Inc. All rights reserved. These products are supplied for laboratory or manufacturing applications only. Facebook is a trademark of Facebook, Inc. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Specifications, terms and pricing are subject to change. Not all products and offers are available in all countries. Please consult your local sales representative for details. PB_2014_01_DIST

Contact your local distributor:

TEMA RICERCA Srlvia XXI Ottobre 1944, 11/240055 Castenaso (BO)ItalyT. +39 051 62 40 700F. +39 051 62 40 706E. [email protected]. www.temaricerca.com

Sign up by June 30, 2014, for tips, applications and promotions about Thermo Scientific Pierce Antibodies and receive 30% off a catalog antibody!

• Learn about new applications and data

• See the latest news about antibody products and website enhancements

• Exclusive promotions

• Delivered quarterly to your inbox

thermoscientific.com/Pierce-abs-signup

Pierce Antibodies eNewsletterSign up – get 30% off!

The 30% discount is limited to one vial of each Product # per order. Additional quantities of the same Product # on the same order will be sold at list price. Not valid with other offers. Antibody offer must be redeemed by July 31, 2014.

Super Substrate Savings Get 4 for the price of 3Reach for Thermo Scientific™ HRP Chemiluminescent Substrates for longer signal duration, broader detection levels and more options.

30%OFF

Get 4 for the

price of 3

Ordering Information

Product # DescriptionPkg. Size Promo Price

32209PROMO Pierce ECL Substrate 4 x 250mL kit

32106PROMO Pierce ECL Substrate 4 x 500mL kit

34080PROMO SuperSignal West Pico Chemiluminescent Substrate

4 x 500mL kit

34078PROMO SuperSignal West Pico Chemiluminescent Substrate

4 x 1L kit

34075PROMO SuperSignal West Dura Extended Duration Substrate

4 x 100mL kit

34076PROMO SuperSignal West Dura Extended Duration Substrate

4 x 200mL kit

34095PROMO SuperSignal West Femto Maximum Sensitivity Substrate

4 x 100mL kit

34096PROMO SuperSignal West Femto Maximum Sensitivity Substrate

4 x 200mL kit

* Offer valid January 2, 2014, through June 30, 2014. Must use special Product # listed here to receive this price. Not valid with other offers and discounts.