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IX INTERNATIONAL SYMPOSIUM

ON PHLEBOTOMINE SANDFLIES

June 28th – July 1

st, 2016 Reims, France

http://www.isops9.org

Scientific program

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The committe thanks for their financial support:

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Monday, June 27th

14:00 – 18:00: registration

Tuesday June, 28th

8:00 – 9:15: registration

9:15 – 10:00: opening ceremony

10:00-12:20 - Inventories, ecology and ethology (oral communications)

10:00 –O1- Phlebotomine sand fly fauna in the Federal District of Brazil. RAPELLO Aline M., DE ANDRADE Andrey J., ROCHA Douglas D A., FERREIRA Jônatas C. B., TIMBÓ Renata V., OBARA Marcos T., GURGEL-GONÇALVES Rodrigo

10:10 – O2- Sand fly fauna (Diptera: Psychodidade: Phlebotominae) and putative vectors of American cutaneous leishmaniasis from Northern Brazil bordering French Guiana. DOS SANTOS Thiago V., DA SILVA Fábio M.M., SANTOS Lucienne A.S., MARQUES Wilson C.S.M., FIRMINO Viviane C., DA SILVA Marcos B., ARAÚJO Sebastiane F., DOS SANTOS Emerson M., PRÉVOT Ghislaine, GINOUVES Marine, PÓVOA Marinete M., RANGEL Elizabeth F.

10:20 – O3- Diversity of phlebotomine sandfly assemblage in a tropical forest of southern Mexico. RODRÍGUEZ-ROJAS Jorge J., REBOLLAR-TÉLLEZ Eduardo A. 10:30 – O4- Phlebotomine sand flies (Diptera: Psychodidae) of Texas, United States. STRUTZ Stavana E., SCHLESENER Cory L., BAGGERLY Ryan, DEPAQUIT Jérôme, PARMESAN Camille.

10:40 - discussion

10:50 – O5- EU-ECDC/EFSA VectorNet Project: Distribution of sand fly species (Diptera: Psychodidae), community analysis and pathogen detection in Balkans. DVORAK V., KASAP O.E., OGUZ G., AYHAN N., VASELEK S., OMERAGIC J., PAJOVIC I., MARTINKOVIC F., MIKOV O., STEFANOVSKA J., PETRIC D., BAYMAK D., ÖZBEL Yusuf, IVOVIC V., VOLF Petr, ALTEN Bülent.

11:00 – O6- First data on sand fly presence in Slovenia. IVOVIĆ V., POPOVIČ A.

11:10 – O7- Distribution and travel distance of Phlebotomus papatasi (Diptera: Psychodidae) in a zoonotic cutaneous leishmaniasis focus, the Northern Negev, Israel. ORSHAN Laor, ELBAZ Shirly, BEN-ARI Yossi.

11:20 – O8- Irrigation in the arid regions of Tunisia impacts the abundance of sand flies population and Leishmania infantum infection. BARHOUMI Walid, FARES Wasfi, CHERNI Saifeddine, DERBALI Mohamed, DACHRAOUI Khalil, CHELBI Ifhem, RAMALHO-ORTIGAO Marcelo, BEIER John C., ZHIOUA Elyes.

11:30 – O9- Phlebotomine Sand Flies (Diptera: Psychodidae) in Morocco: Results of Entomological surveys in five regions, Marrakech- Al Haouz- Chichaoua and Essaouira. ECHCHAKERY Mohamed, OUANAIMI Fouad, BOUSSAA Samia, BOUMEZZOUGH Ali.

11:40: discussion

11:50 – O10- Spatiotemporal analysis of sandfly fauna (Phlebotominae: Psychodidae: Diptera: Psychodidae) in an urban area of visceral leishmaniasis at Kumaun, Uttaranchal region in India. SINGH Naveen Samuel, PHILLIPS-SINGH Doris, LAL Dinesh.

12:00 – O11- Uncharted Phlebotomine sandflies in Gabon. RAHOLA Nil, OBAME Judicael, MAKANGA Boris, AYALA Diego, DEPAQUIT Jérôme, PAUPY Christophe.

12:10: - discussion

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12:20 – 13:00 - Inventories, ecology and ethology (posters oral presentation)

P1-Ecological niche modelling of phlebotominae sand flies and the relation with the distribution of cutaneous leishmaniasis in an endemic region of south-eastern Brazil. FUX Blima, MENEGUZZI Viviane C., DOS SANTOS Claudiney B., MARGONARI Carina, LEITE Gustavo R., FALQUETO Aloísio P2- First recording of Sciopemyia vattierae (Diptera: Psychodidae: Phlebotominae) to the State of Acre, Southeast of the Brazilian Amazon. DE AVILA Márcia M., BRILHANTE Andreia F., GALATI Eunice A.B., BRAZIL Reginaldo P. P3- Sand fly fauna (Diptera: Psychodidae) in endemic area of American Cutaneous Leishmaniasis of Brazilian Amazon. DE AVILA Márcia M., BRILHANTE Andreia F., GALATI Eunice A.B., BRAZIL Reginaldo P. P4- Environmental factors and the occurrence of Nyssomyia whitmani and Lutzomyia cruzi in the Brazilian Central West. FERNANDES Wagner DS, CÂNDIDO Anny K.A.A., BORGES Leandro M., CASARIL Aline E., INFRAN Jucelei DO. M., OSHIRO Elisa T., GOMES Suellem P., FILHO Antônio C. P., PIRANDA Eliane DM., DE OLIVERA Alessandra G. P5 - Normalized Difference Vegetation Index (NDVI) in the characterization of sand flies environments. DE FIGUEIREDO Helen R., CÂNDIDO Anny K.A.A., ENCINA César C. C., DE OLIVEIRA Everton F., MOTTA Jaíza S., INFRAN Jucelei D O. M., CASARIL Aline E., OSHIRO Elisa T., FILHO Antônio C. P., DE OLIVERA Alessandra G. P6- Preliminary information on insular sand flies, in Paraná River, Brazil. DOS SANTOS Barbara A., REINHOLD-CASTRO Kárin R., NEITZKE-ABREU Herintha C., CRISTÓVÃO Edilson C., SILVEIRA Thaís G.V., TEODORO Ueslei P7 - Entomological surveillance in Leishmania transmission areas of leishmaniasis on the border between Brazil and Bolivia. RANGEL Elizabeth F., ZWETSCH Adriana, SANTANA Antonio L. F., GODOY Rodrigo E., SILVA Julia S., SILVA Jose O., ALMEIDA Paulo S., YADON Zaida E. P8- Analysis of feeding preference of sand flies captured in peridomestic habitats in Panama. CABALLERO Ana Rosa, VALDERRAMA Anayansi P9-Why sand flies samplings of a single night are insufficient? An analysis in an urban area of northeast Argentina based on light traps. SANTINI María S., FERNÁNDEZ M. Soledad, QUINTANA María G., CAVIA Regino, SALOMÓN Oscar D. P10-Identification of natural breeding sites of sandflies in an endemic zone of cutaneous leishmaniasis in Argentina. MANTECA ACOSTA Mariana, SANTINI M. Soledad, UTGÉS M. Eugenia, SALOMÓN Oscar D. P11- Update on Phlebotominae fauna from the Chaco region, Argentina. SZELAG Enrique A., ANDRADE-FILHO José D., ROSA Juan R., QUINTANA María G., SALOMÓN Oscar D. P12-Phlebotominae: spatial-temporal distribution in Corrientes city, Argentina. BERROZPE Pablo, SANTINI María S., ARAUJO A.V., LAMATTINA D., SALOMÓN Oscar D. P13- Distribution, abundance, and genetic variability of Lutzomyia longipalpis (Diptera: Psychodidae) in Tartagal city, Salta, Argentina. QUINTANA María G., SANTINI María S., GÓMEZ BRAVO Andrea, FUENZALIDA Ana D., MANTECA ACOSTA Mariana, PECH-MAY Angélica, SALOMÓN Oscar D. P14- Updated distribution records of phlebotomine sand flies (Diptera: Psychodidae) of Spain. LUCIENTES Javier, ESTRADA Rosa, OROPEZA-VELASQUEZ Vladimir, DELACOUR-ESTRELLA Sarah, ALARCÓN-ELBAL Pedro M., RUIZ-ARRONDO José I., MOLINA Ricardo P15- Presence of Phlebotomus (Transphlebotomus) mascittii Grassi, 1908, in northern Spain: first record for the Cantabrian Region and second for the Iberian Peninsula. LUCIENTES Javier, ESTRADA Rosa, OROPEZA-VELASQUEZ Vladimir, DELACOUR-ESTRELLA Sarah, ALARCÓN-ELBAL Pedro M., RUIZ-ARRONDO José I., MOLINA Ricardo P16- Blood feeding behavior of Phlebotomus perniciosus collected in the human leishmaniasis focus of southwest Madrid, Spain, during the period 2012-2015. GONZÁLEZ Estela, MOLINA Ricardo, TELLO Ana, IRISO Andrés, VÁZQUEZ Ángeles, JIMÉNEZ Maribel P17- Ecological aspects of phlebotomine sandflies from Gran Canaria (Canary Islands, Spain) and risk of leishmaniasis transmission. MORILLAS MÁRQUEZ F., GÁLLEGO CULLERÉ Montserrat, MORILLAS MANCILLA M.J., DIAZ SAEZ V., MERINO ESPINOSA G., PESSON B., MUÑOZ BATET C., CORPAS LÓPEZ V., MARTÍN SÁNCHEZ Joaquina.

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P18- First study on phlebotomine sand flies (Diptera, Psychodidae), vectors of Leishmania sp., in Castelo Branco District, Central East region, Portugal. VILELA ML., DE PITA-PEREIRA Daniela, DE ARAUJO-PEREIRA Thais, CRISTÓVÃO José M., MAIA C., CAMPINO Lenea, MAGALHÃES M., RANGEL Elizabeth F., AFONSO Maria O. P19 - Phebotomine sand fly species distribution in Croatia and their implications in Leishmania transmission. BOSNIĆ S., BONGIORNO G., KHOURY C., DI MUCCIO T., GRADONI Luigi, GRAMICCIA Marina, MAROLI Michele P20- Sand Flies (Diptera: Psychodidae) of the mediterranean Africa: inventory and distribution. RHIM Adel, M’GHIRBI Youmna, BRUNHES Jacques, BOUATTOUR Ali P21- An Entomological Survey for Sand flies in Two Counties of Taiwan. SANJOBA Chizu, ÖZBEL Yusuf, SUN Jiamei, KARAKUŞ Mehmet, CHANG Kwang-Poo, TSAI Chi-Wei, WANG Tai-Chuan, MATSUMOTO Yoshitsugu P22- An Inventory of Phlebotomine sand flies from Cambodia. VALLECILLO Thibault, KRUPA Eva, GRATIAUX Julian, ABDOU EL AZIZ Idiyana Rahima, KANG Kimsour, OUK Kalian, GAY Frédérick, IZRI Arezki, DEPAQUIT Jérôme 13:00-14:00 – Lunch

14:00-14:30 – posters visit

14:30-16:00 – laboratory studies (oral communications)

14:30 – O12- Keynote: The unparalleled efficiency of Leishmania transmission by sand fly bites. DEY Ranadhir, ATAYDE Vanessa, JOSHI Amritanshu, ASLAN Hamide, DA SILVA Lais, TOWNSEND Shannon, MENESES Claudio, NAKHASI Hira, OLIVIER Martin, VALENZUELA Jesus, KAMHAWI Shaden.

15:00 – discussion 15:10 – O13 - A comparison of vector competence in different sand fly species to Leishmania donovani. SADLOVA Jovana, MYSKOVA J., PRUZINOVA Katerina, HOMOLA K., YEO M., VOLF Petr.

15:20 – O14 - Leishmania donovani in Phlebotomus argentipes: comparison of development and transmission of amastigote- and promastigote- initiated infections. LESTINOVA Tereza, SADLOVA Jovana, MYSKOVA J., VOTÝPKA Jan, YEO M., VOLF Petr.

15:30 – O15 - Establishing, expanding and certifying a closed working colony of Phlebotomus argentipes (Diptera: Psychodidae) for xenodiagnostic studies at the Kala Azar Medical Research Center, Muzaffarpur, Bihar, India. TIWARY Puja, KUMAR SINGH Shakti, SINGH OP, SACKS David, SUNDAR Shyam, ROWTON Edgar, LAWYER Phillip.

15:40 – O16 - Leishmania tropica development in Phlebotomus sergenti: the effect of temperature, gregarines and geographical origin of sand flies. HLAVACOVA Jana, JANCAROVA M., VOTÝPKA Jan, VOLF Petr.

15:50 – discussion

16:00-16:30 – Coffee break and posters visit

16:30 – O17- Lutzomyia umbratilis population captured in the south of the Negro River is refractory to interaction with Leishmania guyanensis. SOARES R.P, NOGUEIRA P.M, SECUNDINO N.F.C., SANTOS E.F., RÍOS-VELÁSQUEZ C.M., PESSOA F.A.

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16:40 – O18 - Lutzomyia migonei is a permissive vector competent for Leishmania infantum. PRUZINOVA Katerina, GUIMARÃES Vanessa C.F.V., SADLOVA Jovana, VOLFOVA Vera, FILHO Sinval P.B., VOLF Petr.

16:50 – O19- Leishmania proteophosphoglycans regurgitated from infected sand flies accelerates dermal wound repair and exacerbates leishmaniasis via insulin-like growth factor 1-dependent signalling. GIRAUD Emilie, DERRICK Tamsyn, MARTIN Oihane, DILLON Rod J., LESTINOVA Tereza, VOLF Petr, MULLER Ingrid, BATES Paul A., ROGERS Matthew E.

17:00 – discussion 17:10 – O20 - Attraction of Lutzomyia sp. (Diptera: Psychodidae: Phlebotomine) to volatile organic compounds from the skin odour of individuals residing an endemic area for tegumentary leishmaniasis. TAVARES, D. S., MESQUITA, P.R.R., SALGADO, V. R., RODRIGUES, F.M., MIRANDA, J. C., BARRAL, A.

17:20 – O21 -Use of bioassays as an indicator for the presence of pheromone communication in male Lutzomyia migonei (França, 1920) (Diptera: Psychodidae). COSTA Pietra L., MIRANDA Débora, DA SILVA Fernando J., GUIMARÃES Vanessa C.F.V., UNDERHILL Ann, BRAZIL Reginaldo P., HAMILTON James G.C., SHAW Jeffrey, FILHO Sinval P.B.

17:30 – O22 - An oviposition pheromone associated with conspecific eggs is an attractant and stimulant to gravid Phlebotomus papatasi females. CHELBI Ifhem, BOUJÂAMA Anis, ZHIOUA Elyes, HAMILTON James G.C.

17:40 – discussion 17:50 – O23 - Blood derived haem as a potential elicitor of anti-leishmanial activity in the gut of the female sand fly Lutzomyia longipalpis. SILVA José R., SHAWCROSS Emma, DILLON Rod J.

18:00 – O24 - Bacterial communities associated with the digestive tract of wild populations of Lutzomyia evansi: a vector of leishmaniasis in Colombia. VIVERO Rafael J., CADAVID-RESTREPO Gloria E., URIBE SOTO Sandra I., MORENO HERRERA Claudia X., VELEZ Ivan D.

18:10 – O25 -An intergrated overview on the midgut bacterial flora composition of Phlebotomus perniciosus, a vector of zoonotic visceral leishmaniasis in the Western Mediterranean Basin. FRAIHI Wael, FARES Wasfi, SBISSI Imed, PERRIN Pascale, DORKELD Franck, SERENO Denis, BARHOUMI Walid, CHERNI Saifeddine, CHELBI Ifhem, DURVASUL Ravi, RAMALHO-ORTIGAO Marcelo, GTARI Maher, ZHIOUA Elyes.

18:20 – discussion

19:30 – social event: visit of the Cathedral

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Wednesday, June 29th

8:30-9:00 – systematics and phylogeny (posters oral presentation)

P23- What we know on the classification, evolution, and dispersion of Leishmania parasites and sandflies? AKHOUNDI Mohammad, KUHLS Katrin, CANNET Arnaud, VOTÝPKA Jan, MARTY Pierre, DELAUNAY Pascal, SERENO Denis. P24- Illustrated identification key to females of Phlebotominae recorded in the Central-West Region of Brazil using only head and spermathecae. ROCHA Douglas DA., GALATI Eunice A.B., DE ANDRADE Andrey J. P25 - First record of Psychodopygus francoisleponti Zapata, Depaquit & Léon 2012 (Diptera: Psychodidae) in Acre State, Brazil. BRILHANTE Andreia F., DE ÁVILA Márcia M., GODOY Rodrigo E., DE SOUZA Jailson F., CARDOSO Cristiane D O., GALATI Eunice A.B. P26- Morphological and morphometric characters to distinguish females of three sympatric species of the genus Trichophoromyia (Diptera: Psychodidae: Phlebotominae) in a Brazilian Amazonian area. BRILHANTE Andreia F., SÁBIO Priscila B., GALATI Eunice A.B. P27- Metaphase karyotyping organization of Lutzomyia cruzi - preliminary result. DA CUNHA SANTOS Mirella F., BRAGA Natália C., ARAÚJO Douglas, DE FREITAS Lucas O., FERNANDES Wagner, OSHIRO Elisa T., DE OLIVEIRA Alessandra G. P28- Phylogeography and genetic variability of populations of Lutzomyia longipalpis (Diptera: Psychodidae) inferred from ND4 gene. PECH-MAY Angélica, RAMSEY Janine, LIOTTA Domingo, GIULIANI Magali, BERROZPE Pablo, QUINTANA María G., SALOMÓN Oscar D. P29- Sandflies of Congo, new description of the male of Sergentomyia crosarai (Parrot & Wanson, 1946) (Diptera, Psychodidae). NIANG Abdoul Aziz, SENGHOR Massila, TROUILLET Jean, VATTIER-BERNARD Gisèle P30- Sandflies of Senegal. First record of Sergentomyia shoutedeni (Diptera, Psychodidae) in Senegal with description of the male. SENGHOR Massila, NIANG Abdoul Aziz P31- It is time to use a non destructive method for DNA extraction from Phlebotomine sand flies. GRATIAUX Julian, KRUPA Eva, VALLECILLO Thibault, AUGOT Denis, LEHRTER Véronique, GANTIER Jean-Charles, RASPLUS Jean-Yves, DEPAQUIT Jérôme

9:00-10:40 – systematics and phylogeny (oral communications)

9:00-9:20 – O26- Keynote (videoconference). Fossil contribution in the classification of Psychodidae. AZAR Dany 9:20 – discussion 9:30 – O27 - DNA Barcodes of Lutzomyia genus (Dipera: Psychodidae) from a sub-andean forest belonging to Pamplonita River Basin (Norte de Santander, Colombia). HOYOS LOPEZ Richard, CARRERO SARMIENTO Diego

9:40 – O28 - Geometric and linear morphometry as a tool for discriminate cryptic female specimens of Psychodopygus genus Chagasi series. GODOY Rodrigo E., RANGEL Elizabeth F., GALATI Eunice A.B.

9:50 – O29 - LutzodexTM – a digital key for sand flies (Diptera: Phlebotominae) using Android App. ROCHA Douglas DA., DE ALMEIDA Maxwell R., DE ANDRADE Andrey J.

10:00 – discussion

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10:10 – O30 - Phlebotomus (Paraphlebotomus) chabaudi Croset, Abonnenc & Rioux, 1970 and Phlebotomus riouxi Depaquit, Killick-Kendrick & Léger, 1998: synonyms or closely related species? LEHRTER Véronique, DEPAQUIT Jérôme

10:20 – O31 - Beware of Sergentomyia from Southeastern Asia due to untimely synonymies and need to describe new species. DEPAQUIT Jérôme

10:30 – discussion

10:40-11:00 – Coffee break

11:00-11:30 – epidemiology, laboratory studies & modern tools (posters oral

presentation)

P32- The aminosugar galactosamine reduces the trypsinolytic activity of Lutzomyia longipalpis (Diptera: Psychodidae) and promotes Leishmania mexicana and L. infantum development within the sand fly gut. LIMA-SILVA T., CASTRO L.K., BORTOLINI A., PEREIRA M.H., ARAÚJO R.N., GONTIJO N.F., SANT’ANNA M.R.V. P33- Evaluation of different diets for feed larval Nyssomyia neivai (Diptera: Psychodidae: Phlebotominae). FERRARI JÚNIOR Antonio C., DOS SANTOS Kleiton M., FERNANDES Magda F., FERNANDES Wedson D., NEITZKE-ABREU Herintha C., DORVAL Maria E.M.C., DE OLIVERA Alessandra G., GALATI Eunice A.B. P34- Is there oviposition pheromone in Nyssomyia neivai (Diptera: Psychodidae)? GOULART Thais M., DE CASTRO Camila F., OLIVEIRA Wanderson H. C., DA ROCHA SILVA Flávia B., MACHADO Vicente E., ORTIZ Dennys G. S., TOSTA Christiann D., PINTO Mara C. P35 - Experimental infection of Phlebotomus perniciosus by bioluminescent Leishmania infantum using murine model and artificial feeder. CANNET Arnaud, AKHOUNDI Mohammad, GREGORY Michel, MARTY Pierre, DELAUNAY Pascal P36- Exploring the migration of kinetoplastid parasites in sand flies; why are hypopylarian parasites backward in coming forward? VIONETTE-AMARAL Raquel J., NOGUEIRA C.T., GINGER M., DILLON Rod J. P37- Asymptomatic infected dogs are preferentially bitten by Lutzomyia longipalpis in endemic area of American visceral leishmaniasis. BATISTA L.F.S., TOMOKANE T.Y., DA MATTA V.L., PACHECO A., MARCONDES M., LAURENTI Márcia D. P38- Molecular and serological methods in order to evaluate blood meal sources in Phlebotomines sand flies (Diptera: Psychodidae). BAUM Mauricio, DE CASTRO Edilene A., LOROSA Elias S., PINTO Mara C., GOULART Thais M., BAURA Walter, DA COSTA-RIBEIRO Magda C.V. P39- Host feeding preference and molecular screening of Leishmania infection in wild-caught sand flies in an endemic focus Aydın, Turkey. KARAKUŞ Mehmet, PEKAĞIRBAŞ Metin, DEMIR Samiye, EREN Hasan, TÖZ Seray, ÖZBEL Yusuf P40- Anthropophilic behaviour and Leishmania spp. detection in Sergentomyia minuta collected in the human leishmaniasis focus of Madrid, Spain. GONZÁLEZ Estela, TELLO Ana, MOLINA Ricardo, IRISO Andrés, VÁZQUEZ Ángeles, JIMÉNEZ Maribel P41- Molecular detection of Leishmania tropica parasites kDNA from naturally infected sand flies in a new foothill endemic area, southeast Iran. MOEMENBELLAH-FARD Mohammad Djaefar, AZIZI Kourosh, FAKOORZIBA Mohammad Reza, DABAGHMANESH Tahereh, AHMADYOUSEFI-SARHADI Mohammad P42- Epidemiology of Cutaneous Leishmaniasis in the municipality of Brasiléia, state of Acre: Study on the sandy fly fauna. DE ARAUJO- PEREIRA Thais, DE PITA-PEREIRA Daniela, BOITÉ Mariana, MARTINS Daniella A., DA COSTA-REGO Taiana A. N., PINTO Israel DS., MOREIRA Regina B., FUZARI Andressa A., ANDRADE-FILHO José D., OLIVEIRA Marcia, BRAZIL Reginaldo P., BRITTO Constança

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P43- Seasonal dynamics, evolution of Leishmania infantum infection rates, and host-feeding preferences of Phlebotomus perniciosus in the focus of human leishmaniasis in Madrid region, Spain (2012 - 2014). MOLINA Ricardo, GONZÁLEZ Estela, HERNÁNDEZ Sonia, MARTÍN-MARTÍN Inés, JIMÉNEZ Maribel P44- Molecular tools for the identification of Phlebotomine sand flies and detection of Leishmania spp. parasites in Misiones province, Argentina. MOYA Sofía L., GIULIANI Magalí G., MANTECA ACOSTA Mariana, SALOMÓN Oscar D., LIOTTA Domingo J.

11:30-12:30 – Systematics round table.

12:30-13:30 – Lunch

13:30-14:00 – posters visit

14:00-16:30 – epidemiology (oral communications)

14:00 – O32- Keynote: Can Sergentomyia spp. play a role in the transmission of human and animal leishmaniases? MAIA Carla

14:20 – discussion 14:30 – O33 - Molecular analysis of parasite, vector and blood meal DNA from field-caught sand flies in a Moroccan focus of cutaneous leishmaniasis: Genetically heterogenous Leishmania tropica in Phlebotomus sergenti as a mono-specific and multi-host feeding vector. AJAOUD Malika, ES-SETTE Nargys, CHARREL Rémi, LAAMRANI-IDRISSI Abderahmane, RIYAD Myriam, LEMRANI Meryem.

14:40 – O34 - Sand flies abundance, ecology and oviposition preferences in Bihar, India. GARLAPATI Rajesh B, MUKHERJEE Shanta, CHAUBEY Rahul, RAHAMAN Tahfizur, BABELE Piyoosh, CHOWDHURY Akanksha, PRAKASH Suman, KUMAR Vinod, KUMAR Mukesh, FRANCKOWIAK Gregory, SOMERS Dan, BRILEY Lindsay, WAGNER Katelyn, HULKE Jenna, CALVERT McCall, POLYAKOVA Larisa, POCHÉ David, POCHÉ Richard.

14:50 – discussion 15:00 – O35- Keynote: Phlebotomine sandflies vectors of arbovirus: review and recent data. CHARREL Rémi

15:20 – O36 - Cocirculation of sand fly-transmitted phleboviruses and Leishmania infantum in a zoonotic visceral Leishmaniais focus located in arid bio-gepgraphical areas of Central Tunisia. FARES Wasfi, DACHRAOUI Khalil, BARHOUMI Walid, CHERNI Saifedine, FRAIHI Wael, SAKHRIA Sonia, CHELBI Ifhem, CLOS Joachim, BECKER Stefanie, ZHIOUA Elyes.

15:30 – O37 - Sand fly fever in Iran: from past up to Dashli virus isolation as a new Sicilian like virus. MOIN-VAZIRI Vahideh, ALKAN Cigdem, BADAKHSHAN M., RAHBARIAN N., DE LAMBALLERIE Xavier, CHARREL Rémi.

15:40 – O38 - Emergence of Toscana virus and other Sandfly-borne phleboviruses in Central Tunisia. DACHRAOUI Khalil, FARES Wasfi, BICHAUD Laurence, BARHOUMI Walid, BEIER John C., DERBALI Mohamed, CHERNI Saifeddine, DE LAMBALLERIE Xavier, CHELBI Ifhem, CHARREL Rémi, ZHIOUA Elyes.

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15:50 – discussion 16:10 –O39 - Sand fly fauna of Palmas, state of Tocantins, Brazil: occurrence in different environments and natural infection by trypanosomatids. MACHADO Tâmara D.O., FERREIRA Tauana S., SANTOS JUNIOR Alcinei S., SALES Nathyla M. C., TIMBÓ Renata V., VITAL Tamires E., MINUZZI-SOUSA Thaís T. C., DE ANDRADE Andrey J., OBARA Marcos T., GURGEL-GONÇALVES Rodrigo. 16 :20 – O40 - First Detection of an unknown Trypanosoma DNA in a Phlebotomine sand fly collected from southern Thailand. PHUMEE Atchara, TAWATSIN Apiwat, THAVARA Usavadee, PENGSAKUL Theerakamol, THAMMAPALO Suwich, DEPAQUIT Jérôme, GAY Frédérick, SIRIYASATIEN Padet 16:20 – discussion

16:30 - 17:30 – Coffee break, posters visit, sandflies colonization workshop (room

B1036, 1st floor).

19:30 – social event: visit of the Mumm cellar and gala dinner.

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Thursday, June 30th

8:30-10:30 – epidemiology and control (oral communications)

8:30 – O41 - Overview and an update of the current knowledge and perspectives on sandfly research in Mexico. REBOLLAR-TÉLLEZ Eduardo A., IBÁÑEZ-BERNAL Sergio I., RODRÍGUEZ-ROJAS Jorge J., MOO-LLANES David A., PECH-MAY Angélica, MONTES DE OCA-AGUILAR Ana C., MIKERI-PACHECO Oscar, BERZUNZA-CRUZ Miriam, BECKER-FAUSER Ingeborg, RAMSEY Janine, IBARRA-CERDEÑA Carlos, RODRÍGUEZ-MORENO Ángel, STEPHENS Christopher, SÁNCHEZ-CORDERO Victor, CASTILLO-VERA Alfredo, GONZÁLEZ Camila, ARQUE-CHUNGA Wilfredo, ESCOBEDO-ORTEGÓN Javier, PASOS-PINTO Silvia, SÁNCHEZ-GARCÍA Laura.

8:40 – O42 - Abundance of Lutzomyia longipalpis (Diptera, Psychodidae) in a kennel and its surroundings on a highly endemic visceral leishmaniosis area in São Paulo State, Brazil. CUTOLO, A.A., BRIGUENTE, K.B.S., MOTOIE, G., PIGOZZI, C.E.J., NEVES, B.L., MENZ, I., PEREIRA-CHIOCCOLA, V.L.

8:50 – O43 - Canine visceral leishmaniasis in the São Paulo Metropotian area dissociated of Lutzomyia longipalpis: Pintomyia fischeri as potential vector of Leishmania infantum chagasi. OVALLOS Fredy G., GALATI Eunice A.B.

9:00 – discussion

9:10 – O44 - The emergence and spread of leishmaniases in the borders of Argentina, Brazil, Paraguay and Uruguay. SALOMÓN Oscar Daniel, QUINTANA María Gabriela, SANTINI María Soledad, MARTÍNEZ Nidia, ROJAS DE ARIAS Antonieta, THOMAZ-SOCCOL Vanete, GONÇALVES André Luiz, BISETTO Júnior Alceu, WILLAT Gabriela, CALEGARI Luis, BASMADJIAN Yester, YADON Zaida E., IDRC Project #107577 team.

9:20 – O45 - Evaluation of the synthetic sex pheromone, (S)-9-methylgermacrene-B, for recruitment and monitoring of Lutzomyia longipalpis (Diptera: Psychodidae) in an environmental reserve in Rio de Janeiro, Brazil. BARBOSA Vanessa DA., RODRIGUES Andressa A.F., HAMILTON James G.C., BRAZIL Reginaldo P. 9:30 – O46 - Synthetic Pheromone and Long Lasting Insecticidal Nets (LLINs) as a new control strategy for Lutzomyia longipalpis (Diptera: Psychodidae), the vector of Leishmania (Leishmania) infantum. BARBOSA Vanessa D A., DE SOUZA Cristian F., HAMILTON James G.C., BRAZIL Reginaldo P.

9:40 – discussion

9:50 – O47 - Identifying the Yeast community in the sand fly Phlebotomus perniciosus: towards a strategy for yeast-mediated biological control of vector-borne diseases. MARTIN Elena, VAROTTO BOCCAZZI Ilaria, BONGIORNO Gioia, DE MARCO Leone, GRADONI Luigi, BASILICO Nicoletta, COMAZZI Stefano, RICCI Irene, EPIS Sara.

10:00 – O48 - Targeting sandfly control by the use of systemic insecticides presented to mammalian reservoir hosts of ZCL and VL: a review of recent studies. POCHÉ Richard, GARLAPATI Rajesh B., POCHÉ David.

10:10 – O49 - Systemic insecticides used in dogs: potential candidates for sandfly control? ARES GOMEZ Sonia, PICADO Albert.

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10:20 – discussion

10:30-11:00 – Coffee break, posters visit.

11:00-12:20 – epidemiology and control (oral communications)

11:00 – O50 - Field evaluation of rodent bait treated with fipronil for feed through and systemic control of Phlebotomus papatasi. DERBALI Mohamed, DACHRAOUI Khalil, CHERNI Saifeddine, BARHOUMI Walid, CHELBI Ifhem, POCHÉ Richard, ZHIOUA Elyes.

11:10 – O51- A comparative study on the phostoxin and zinc phosphide effectiveness to the control zoonotic cutaneous leishmaniasis in central Iran. VEYSI Archad, VATANDOOST Hassan, YAGHOOBI-ERSHADI Mohammad Reza, JAFARI Reza, ARANDIAN Mohammad Hossein, HOSSEINI Mostafa, ABDOLI Hamid, HEIDARI Kamal, SADJADI Ali, FADAEI Reza, SHIRZADI Mohammad Reza, AKHAVAN Amir Ahmad.

11:20 – O52- Repellent efficacy of a new combination of fipronil and permethrin against the main vector of visceral canine leishmaniosis in the Americas (Lutzomyia longipalpis). CUTOLO, A.A., OVALLOS, F.G., NEVES, E.S., SOSSAI, S., VIEIRA, M.M.F., SILVA, F.O., CHESTER, S.T., FANKHAUSER, B., SOLL, M.D.

11:30 – discussion

11:40 – O53 - Efficacy of environmental management along with indoor residual spraying of insecticides for controlling sand flies. DINESH Diwakar Singh, SEEMA Kumari, HASSAN Faizan, KESARI Shreekant, KUMAR Vijay, DAS Pradeep.

11:50 – O54 - Molecular and biochemical characterization of insecticide resistance in Phlebotomus and Lutzomyia sand flies. BERNHARDT Scott A., DENLINGER David S., GOMPERT Zachariah, CRESWELL Joseph S.

12:00 – O55 - Resistance of Phlebotomus argentipes (Diptera: Psychodidae) a vector of visceral leishmaniasis to insecticides in India. SINGH Ram.

12:10 – discussion 12:20- 13:00: epidemiology and control (posters oral presentation)

P45- Impact of climate change on the geographic distribution areas of sandfly vector of leishmaniasis in the pre-Saharan zones of North Africa and the need of good government strategies. KAHIME Kholoud, MESSOULI M., BOUSSAA Samia, BOUMEZZOUGH Ali. P46- Role of irrigation areas in zoonotic cutaneous leishmaniasis transmission in central Tunisia: Investigation of Phlebotomus (Phlebotomus) papatasi distribution and Leishmania infection rate. GHRAB Jamila, CHOUIHI Emira, DAABOUB Jabeur, BOURATBINE Aida, CHAHED Mohamed K. P47- Dynamics of Laroussius populations and Leishmania infection rate of female sandflies in an endemic visceral leishmaniasis region, Tunisia, North Africa. BENABID Meriem, RHIM Adel, BEN ROMDHANE Rania, ZERZRI Manel, BOURATBINE Aida

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P48- Epidemiologic survey of phlebotomine vectors in a canine leishmaniasis endemic area in Spain. VELEZ R., BALLART Cristina, DOMENECH E., CAIRÓ J., PORTÚS Montserrat, GÁLLEGO Montserrat P49- Evidence for stable endemic sandfly populations in the light of migration streams into Austria. OBWALLER Adelheid G., KARAKUŞ Mehmet, POEPPL Wolfgang, TOZ Seray, ÖZBEL Yusuf, ASPÖCK Horst, WALOCHNIK Julia P50- Absence of Leishmania-infected phlebotomines in gallery forests of the Federal District of Brazil. RAPELLO Aline M., MINUZZI-SOUSA Thaís T. C., VITAL Tamires E., FERREIRA Tauana S., TIMBÓ Renata V., DE ANDRADE Andrey J., GURGEL-GONÇALVES Rodrigo P51- Vectors of the subgenus Leishmania (Viannia) in the Tapajós national forest reserve located in the lower Amazon Region of Brazil. DE SOUZA Adelson A.(†), DOS SANTOS Thiago V., JENNINGS Yara L., ISHIKAWA Edna A., BARATA Iorlando, SILVA Maria DG.(†), LIMA José A., SHAW Jeffrey, LAINSON Ralph(†), SILVEIRA Fernando P52- Natural transovarial and transstadial transmission of Leishmania infantum in Rhipicephalus sanguineus (Acari :Ixodidae). AZIZI Kourosh, ASGARI Qasem, MOEMENBELLAH-FARD Mohammad Djaefar, SOLTANI Aboozar, DABAGHMANESH Tahereh P53- Molecular Epidemiology of phlebovirus in four provinces in Morocco. ES-SETTE Nargys, AJAOUD Malika, CHARREL Rémi, LEMRANI Meryem P54- Phleboviruses circulating in sandflies in Emilia-Romagna region (Northern Italy) in 2013-2015. CALZOLARI Mattia, BELLINI Romeo, BONILAURI Paolo, PINNA Marco, DEFILIPPO Francesco, DOTTORI Michele, ANGELINI Paola. P55- Isolation of Piura virus, an insect-specific negevirus, from Lutzomyia evansi in Colombia. CONTRERAS-GUTIÉRREZ María Angélica, GUZMAN Hilda, NUNES Marcio RT., URIBE SOTO Sandra I., VIVERO Rafael J., VÉLEZ Iván D., VASILASKIS Nikos, TESH Robert B. P56- Characterization of susceptibility of Phlebotominae (Diptera: Psychodidae) to the insecticide, alpha-cypermethrin. ROCHA Douglas DA., DE ANDRADE Andrey J., REINALDO Luciana M., OBARA Marcos T. P57- Evaluation of the level of knowledge of public health professionals regarding the vector of visceral leishmaniasis and its control measures. MARTINS Anna A.P., FUGA Mariana, DE OLIVERA Alessandra G., SANTOS Mirella F. D C. - P58- Reduced Leishmania infantum parasitic loads in humans exposed to sand fly bites in the Amazon region of Brazil. LAURENTI Márcia D., CHAVES Luis F., WARBURG Alon, SILVEIRA Fernando T. - P59- Comparison of various recombinant salivary proteins as epidemiological markers for dog exposure to Phlebotomus perniciosus in different localities in Italy, Portugal and Spain. WILLEN Laura, KOSTALOVA Tatiana, POLANSKA Nikola, LESTINOVA Tereza, MAIA Carla, SUMOVA Petra, VLKOVA Michaela, FIORENTINO Eleonora, SCALONE Aldo, OLIVA Gaetano, VERONESI Fabrizia, CRISTÓVÃO José M., COURTENAY Orin, CAMPINO Lenea, GRADONI Luigi, GRAMICCIA Marina, BALLART Cristina, GÁLLEGO Montserrat, VOLF Petr. - P60- Can we identify Leishmania super-spreaders to reduce transmission to sand fly vectors? LISON Aurore, REED Steve, COURTENAY Orin - P61- Vector control using long-lasting insecticidal nets against Kala-azar in Bangladesh. SANJOBA Chizu, ÖZBEL Yusuf, TOJO Bunpei, NOİRİ Eisei, MATSUMOTO Yoshitsugu - P62- Analysis of gene expression in a Lutzomyia longipalpis-derived cell line. CORTES Luzia M. C., MELO Barbara C. A., SOUZA-SILVA Franklin, PEREIRA Bernardo A. S., BELLO Felio J., MOREIRA Otacilio C., DE PITA-PEREIRA Daniela, BRITTO Constança, ALVES Carlos R.

13:00-14:00 – Lunch

14:00-15:30 – Posters visit, sandflies colonization workshop (room B1036, 1st floor).

14

15:30- 16:30: epidemiology and control (oral communivations)

15:30 – O56- Evaluation of the spatial relationship between area of insecticide treatment and location of Leishmaniasis cases using geographical information systems in Adana, Turkey. KAVUR Hakan, ARTUN Ozan, KOCA Kenan.

15:40 – O57 - Manipulation of sandfly distributions within the peridomestic environment, and implications for the control of vector borne disease. DILGER E., BORGES-ALVES G., CARTER V., HEREDEDIA M.G., NUNES C.M., GARCEZ L.M., BRAZIL Reginaldo P., HAMILTON James G.C., COURTENAY Orin.

15:50 – discussion 16:00 – O58- KalaCORE research on the efficacy of control measures against Phlebotomus orientalis, the principal vector of visceral leishmaniasis in East Africa. ELNAIEM Dia-Eldin, OSMAN Omran F., LEMMA Wossenseged, ELHADI Hanan A. A., NOUR Bakri Y. M., KHALID Noteila M., YIMER Mulat, ALVAR Jorgi, COURTENAY Orin.

16:10 – O59 - Visceral leishmaniasis on the Indian subcontinent: modelling the dynamic relationship between vector control schemes and vector life cycles. POCHÉ David, GRANT William, WANG Hsiao-Hsuan

16:20 – discussion 16:30 – coffee break and posters visit 18:00 – social event: meeting with the mayor at City Hall around a glass of Champagne.

15

Friday, July 1st

9:00 -10:40 – modern tools for sand flies studies (oral communications)

9:00 – O60- Leishmania HASP and SHERP genes are required for in vivo differentiation, parasite transmission and host virulence attenuation. DOEHL Johannes S. P., SÁDLOVÁ Jovana, ASLAN Hamide, METANGMO Sonia, VOTÝPKA Jan, KAMHAWI Shaden, VOLF Petr, SMITH Deborah F.

9:10 – O61- A glance on what Leishmania infantum chagasi expresses inside Lutzomyia longipalpis. LOZA-TELLERIA Erich, DA SILVA Thais L., FARIAS João R. O., TRAUB-CSEKÖ Yara M.

9:20 - discussion 9:30 – O62 - Lutzomyia longipalpis TGF-β has a role in Leishmania infantum chagasi survival in the vector. DI-BLASI T., LOZA-TELLERIA Erich, MARQUES C., MACEDO-COUTO R., NEVES M., TEMPONE A.J., RAMALHO-ORTIGÃO M., TRAUB-CSEKÖ Yara M.

9:40 – O63 - Novel method to quantify leishmania metacyclic promastigotes delivered by individual sand fly bite reveals the efficiency of parasite transmission. GIRAUD Emilie, MARTIN Oihane, ROGERS Matthew E.

9:50 – discussion 10:00 – O64 - Blood feeding effect on Phlebotomus papatasi SP15 and SP44 salivary transcripts. HOSSEINI-VASOUKOLAEI Nasibeh, AKHAVAN Amir Ahmad, JEDDI-TEHRANI Mahmood, IDALI Farah, KHAMESIPOUR Ali, YAGHOOBI-ERSHADI Mohammad Reza, KAMHAWI Shaden, VALENZUELA Jesus G.

10:10 – O65 - Phlebotomus orientalis salivary proteins and antigens. ROHOUSOVA Iva, WARBURG Alon, VOLF Petr.

10:20 – O66- Parity/nulliparity and sand fly salivary gland gene expression. HOSSEINI-VASOUKOLAEI Nasibeh, AKHAVAN Amir Ahmad, JEDDI-TEHRANI Mahmood, IDALI Farah, KHAMESIPOUR Ali, YAGHOOBI-ERSHADI Mohammad Reza, KAMHAWI Shaden, VALENZUELA Jesus G.

10:30 – discussion

10:40-11:20 – Coffee break, posters visit.

11:20 -12:10– modern tools for sand flies studies (oral communications)

11:20 – O67 - Different approaches for further application of MALDI-TOF mass spectrometry for species identification of phlebotomine sand flies species identification. HLAVACKOVA Kristýna, DVORAK V., HALADA P., VOLF Petr.

11:30 – O68- MALDI-TOF protein profiling as a method of choice for high-throughput species identification of sand flies - an example from the Balkan. DVORAK V., HLAVACKOVA Kristýna, HALADA P., ALTEN Bülent, IVOVIC V., OMERAGIC J., PAJOVIC I., MARTINKOVIC F., MIKOV O., STEFANOVSKA J., VOLF Petr.

16

11:40 – discussion

11:50 – O69 - New generation sequencing (NGS) as a tool for identification of pooled sand flies. AYHAN Nazli, DVORAK Vit, PIORKOWSKI Géraldine, ALKAN Cigdem, de LAMBALLERIE Xavier, VOLF Petr, CHARREL Rémi N.

12:00 – discussion

12h10 – 13h30 – lunch

13h30: closing ceremony and designation of the place and date of ISOPS X