Journal of Research in Engineering and Applied Sciences

ISOLATION AND IDENTIFICATION OF MYCOFLORA FROM PETROLEUM CONTAMINATED

SOILS IN PUDUCHERRY REGION

1 2Research Supervisor, Research ScholarKanchi Mamunivar Centre for Post Graduate Studies

2Email: { yoursarun85@gmail.com}

Abstract

1. Introduction

2. Materials and methods

2.1. Study site

2.2 Preparation of samples

2.3 Spread plate methods

34

2.3 Enumeration of colonies

2.4 Identification of organisms

2.5 Isolation of soil mycoflora

3. Results and discussion

Table1: Physical characterization of various soil samples

S. No.

Physico-chemcial parameters

Soil sample locations

S1

S2

S3 S4 S5 S6

1

pH

8.1

7.4

7.8 8.2 6.8 8.0

2

Electrical conductivity

(dSm-1 )

0.17

0.10

0.12 2.10 0.11 0.24

3

Organic carbon

0.44

0.22

0.22 0.31 0.38 0.67

4

Soil texture

Sandy loam

Sandy loam

Sand SandSandy loam

Sandy loam

5

Available Nitrogen (Kg ha-1)

71.83

64.90

120.65 103.13 30.33 156.88

6

Phosphorus (Kg ha-1)

19.57

16.87

19.54 19.72 17.51 20.23

7

Potassium (ppm)

8.97

33.45

5.56 31.23 8.90 14.0

8

Bulk density (gm/cc)

1.51

1.46

1.32 1.67 1.22 1.32

9

Sodium (ppm)

42.65

599.0

NA NA 22.77 609.14

10 Calcium (meq/100 g) 0.12 0.11 NA NA 0.44 0.53

11 Magnesium (meq/100 g) 0.14 0.15 NA NA 0.13 0.19

12 Copper (ppm) 2.32 1.14 NA NA 1.5 1.5

13 Zinc (ppm) 2.0 1.6 NA NA 1.9 1.0

14 Manganese (ppm) 3.9 2.5 NA NA 5.6 543

15 Iron (ppm) 25.7 27.8 NA NA 25.7 7.3

Table 2: Mycoflora of various soil samples

S. No.

Phylum Class Order/Group

Genus with no. of species

1

Ascomycota

Eurotiomycetes

Eurotiales (28)Penicillium (14)

2

Sordariomycetes

Hypocreales Cladosporium (6) Fusarium (12)

3

Saccharomycetes

Saccharomycetales

Geotrichum (5)

4

Zygomycota

Zygomycetes

Mucorales Absidia (11)Mucor (3)Rhizopus (12)

5 Basidiomycota Urediniomycetes Sporidiales Torula (3)Pseudotorula (2)

6 Agaricomycetes Cantharellales Rhizoctonia (2)

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4. Conclusion

References

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