is leading us into a prosperous future in the 21st century · 2018. 3. 12. · kribb achieved great...

is leading us into a prosperous futurein the 21st century

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Contents

04Introduction

14Divisions

68Biotechnology R&DInfrastructure

92Appendices

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Division of Leading Research

Division of BioIntegration Research

Division of Molecular Therapeutics

Division of Biomaterials Science

Division of Biotechnology R&BD

Division of Ochang R&D

Jeonbuk Branch Institute

Biological Resource Center

Daejeon-KRIBB-FHCRC

Research Cooperation Center

Korea Biosafety Clearing House

Biotech Policy Research Center

KOrean BioInformation Center(KOBIC)

International Biological Material

Research Center

The 21st Century Frontier R&D Program

International Cooperation

Technology Commercialization

Support for Technology Information

Outstanding Research Achievements

List of Patents Registered Overseas

List of Technology Transfer

List of Research Projects

Events

Researcher Index

Location

Message from the President

Mission and Vision,

Key R&D Areas

Management Objectives

General Information

Organization

Yearly Progress

| 04 Introduction | 14 Divisions | 68 Biotechnology R&D Infrastructure | 92 Appendices

NK cell therapy, the development of a diagnostic tool for gastric cancer using protein biomarkers(International J. of

Cancer, 2007.02), and the discovery of a hyper sensitive cancer biomarker have qualitatively altered during cancer

progression(Molecular & Cellular Proteomics, 2007.12). Our latest achievements have proven excellence both from

the qualitative and quantitative perspectives compared with the previous year's publications and patents.

Now, KRIBB, which is undergoing a remarkable period of growth, is living up to its new motto 'KRIBB High-Five'

seeking rebirth as 'a premium technical research institute capable of leading the world into a new era of

biotechnology innovation'. Every researcher at KRIBB is fully committed to ensuring better public health and

pursuing the development of the bio industry.

Sang Ki Rhee, Ph.D.President & CEO

The Korea Research Institute of Bioscience and Biotechnology(KRIBB)

is the government-funded research institute dedicated to state-of-

the-art bioscience and biotechnology. The Korean government has

been systematically promoting biotechnology through the national

plan [Biotech 2000] (1994~2006), which has made a remarkable

progress to date. [Bio-Vision 2016] envisions designed to make Korea

one of the world's top seven biotechnology powerhouses by 2016.

KRIBB achieved great results in 2007. We have built up a strategic

partnership with the global pharmaceutical company, Pfizer(2007.06)

and also established the International Biological Material Research

Center. Furthermore, the approval of a research-based clinical trial of

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Messagefrom the President

Mission & Vision

Key R&D Areas

ManagementObjectives

Vision

Result

Strategy

Bio-Pharmaceuticals

Bio-Materials

Bio-Nanotechnology

Bio-Informatics

To develop next generation bio-pharmaceuticals by using genome and proteomeinformation, and stem cell and bio-organs by regenerating cells

To develop new functional materials by using genome information, and clean andenvironmental biomaterials

To develop cutting-edge fusion bioscience and technology (biochip, biosensor)

To discover bio information related to diseases, to collect bio information, and tointerpret bio structures and functions

"KRIBB High-Five 2016"The premium research institute leading global biotechnology innovation

Create Global Elite5 world-class researchinstitutes5 world-class scientists

Create Top Brand5 items for technologysource of industrialization

Contribute Bio Economy50 trillion economy ripple effect50 billion won royalty

KRIBB High-Five 2016Pride

KRIBBElite

KRIBBConnectKRIBB

Safe KRIBB

Global KRIBB

Quality Management for a superb performanceWe have implemented 'quality management' in order to ensure great results in our four main Research

Areas - bio-pharmaceuticals, bio-materials, bio-nanotechnology, and bio-informatics. In order to

manage the organization's objectives and facilitate our rebirth as an organization focused on policies,

we have reformed the research organization and made a commitment to promote the national

economy and public welfare by pursuing significant research achievements.

Innovation Management to strengthen our innovative abilityWe, as a government-funded research institute, have established and promoted five innovation

policies, the 'KRIBB High-Five', with the goal of improving research and management efficiency.

Pride KRIBB Establishing an organization culture based on unity and faithElite KRIBB Seeking out and cultivating the bio-stars of the next generationConnect KRIBB Creating new, up-to-date businessSafe KRIBB Creating a safe environment for researchers on the front burnerGlobal KRIBB Raising the research institute's status and profile throughout the world

Open Management - laying the foundations for another leap forwardFor our second leap forward, we have been promoting the business of the Ochang campus and the

Jeonbuk Branch Institute, and performing our role as the primary agent of domestic bio-clusters. We

have also been working on raising public awareness of the organization and buiding a global

cooperation network through an ongoing process of exchange with foreign organizations.

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General Information

Foundation BasisArticle 8, Act on the Establishment, Management and Promotion of Government-funded Research Institutions

ObjectivesResearch and development in bioscience and biotechnology and support for research projects Domestic and overseas cooperation among industry, academia and research institutes and distribution of the results

MissionResearch institute for realizing a bio-society in the 21st century

FunctionResearch on bioengineering and the distribution of technology (bio-pharmaceuticals, bio-materials, bio-nanotechnology, bio-informatics)Public infrastructure support for industry, academia, research institutes, and the government(public infrastructure, cooperation among industry, academia and research institutes, think-tank for national policy)

History

1985. 02

1989. 06

1990. 07

1990. 12

1995. 03

1999. 05

2001. 01

The Genetic Research Center(GRC) established as a spin-off institute of the Korea Advanced Institute of Science and Technology(KIST)

GRC transferred to the Korea Institute of Science and Technology(KAIST)

GRC moved to Daedeok Science Town

GRC changed its name to the Genetic Engineering Research Institute(GERI)

GERI changed to the Korea Research Institute of Bioscience and Biotechnology(KRIBB)

KRIBB became an independent entity

KRIBB became an independent legal entity under the umbrella ofthe Korea Research Council of Fundamental Science and Technology(KRCF)

Human Resources

Budget (Unit: Millions of Won) Facilities (Area: m2)

Descriptions

Regular employees

Executives

1

Researchers

176

Engineers

46

Administrators

26

Technicians

46

Total

295

Descriptions

Government fundsMinistry of Commerce, Industry and Energy fundsInstitute revenues

Government-funded projectsPrivately-funded projectsRoyaltiesMiscellaneous

Total

Revenue

Budget

50,832

2,291

52,18348,5331,850

5001,300

105,306

Expenditure

Descriptions

Personal expensesDirect expenses

Institute projectsSpecific research projectsResearch for other organizationsPrivately-funded projectsGeneral purpose projects

Indirect expenses Working expensesInstallation expenses

OthersLoansMiscellaneous

Total

Budget

21,881

65,02118,17622,41114,9491,4378,048

17,1125,450

11,662

1,292792500

105,306

Daejeon Main Campus

Site Building Research and support

Main buildingResearch buildingResource buildingVenture buildingNative plant building(1)Native plant building(2)Cafeteria buildingGreenhouse research building, etc.

Residence SingleSuperintendence APTDormitory

Ochang Campus

Site Building Research

National Primate Research CenterBio-Evaluation Center


Site Building Research

Research buildingAdministration building and dormitory

100,978

51,552

44,67311,87117,0086,5543,0441,1341,0772,6461,339

6,879909991

4,979

212,258

14,235

14,2354,7749,461

18,098

8,551

8,5515,7402,811

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10

OrganizationPresident Auditor Assistant Auditor

Vice President

Biological Resource Center

Division ofBiotechnology R&BD

Division of Leading Research

Division ofBioIntegration Research

Division ofMolecularTherapeutics

Division ofBiomaterials Science

Divison of R&D Policy

Division of Planning & Management

Division of General Administration

Division of Ochang R&D


Intellectual PropertyManagement DevelopmentBiotechnology ProcessEngineeringDepartmentBiotechnology ProcessDevelopmentDepartment

Systemic Proteomics Research CenterStem Cell Research Center

BioNanotechnology Research Center

Genome Research Center

TherapeuticAntibody Research CenterMetabolic’Syndrome Research Center

Medical Genomics Research Center

Disease Model Research Center

Regenerative Medicine Research CenterOmics andIntegration Research CenterTranslational Research Center

Molecular Cancer Research Center

Natural Medicines Research Center

FunctionalMetabolite Research Center

Plant Genome Research Center

SystemsMicrobiology Research CenterEnvironmental Biotechnology Research Center

Innovation & Policy Department- Innovation & Policy

Research Team- Innovation &

Management Team

Public Relations & Inter-national CooperationDepartment

Planning & Budget Section

R&D Management Section

Computer & InformationSection

General Affairs Section

ProcurementSection

Facilities & Securities Section

AccountingSection

Bio-Evaluation Center

National PrimateResearch Center

Ochang General Administration Section

Molecular Bioprocess Research CenterBioindustry Research Center

Jeonbuk General Administration Section

Daejeon-KRIBB -FHCRC ResearchCooperation Center

KoreaBiosafetyClearing House

Biotech PolicyResearchCenter

International Biological MaterialResearch Center

KOrean Bio InformationCenter (KOBIC)

MicrobialGenomics & Applications Center

Plant Diversity ResearchCenter

Human Genome Functional Analysis Center

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12

Yearly Progress

1. Personnel

2. Budget Unit : Millions of won

3. Research Expenses Unit : Millions of won

4. Publication Unit : Items

5. Patents Unit : Items

6. Technology Transfer Unit : Items

Regular EmployeesSpecial Service Interns

Total

‘00228297525

‘07295639934

‘06295530825

‘05297547884

‘04300510810

‘03290461751

‘02280353633

‘01243343586

‘00 ‘01 ‘02 ‘03 ‘04 ‘05 ‘06 ‘07

‘00 ‘01 ‘02 ‘03 ‘04 ‘05 ‘06 ‘07

‘00 ‘01 ‘02 ‘03 ‘04 ‘05 ‘06 ‘07

‘00 ‘01 ‘02 ‘03 ‘04 ‘05 ‘06 ‘07

‘00 ‘01 ‘02 ‘03 ‘04 ‘05 ‘06 ‘07

‘00 ‘01 ‘02 ‘03 ‘04 ‘05 ‘06 ‘07

Total‘00

44,134‘07

105,306‘06

98,257‘05

95,594‘04

76,198‘03

64,573‘02

62,172‘01

53,244

GovernmentInstitutes

PrivateOthers

Total

‘0031,6579,0251,626

4542,353

‘0750,68640,6991,401

-92,756

‘0650,94135,2101,299

-87,450

‘0547,04128,546

702200

76,489

‘0444,00822,860

709905

65,482

‘0337,88820,268

757740

59,653

‘0243,08510,044

444-

53,573

‘0137,5259,5801,023

3948,167

DomesticOverseas

Total

‘00120208328

‘07129287416

‘06141360501

‘05136303439

‘04117271388

‘03121229350

‘02106170276

‘01116188204

DomesticOverseas

Total

‘0019-

19

‘0713215

‘0613-

13

‘0515-

15

‘048-8

‘0311-

11

‘02213

‘0120-

20

Domestic ApplicationRegistration

Overseas ApplicationRegistration

‘008667

1714

‘07184126

4711

‘06147139

4613

‘05127116

2310

‘0413588

2520

‘0311364

1928

‘027364

1217

‘017965

89

525

586

934

825884

810

751

633

44,13

4

98,25

7

105,3

06

76,19

8

64,57

3

62,17

2

53,24

4

95,59

4

87,45

0

92,75

6

65,48

2

59,65

3

53,57

3

48,16

7

76,48

9

328

304

416

501

439

388

350

276

19

20

15

13

15

8

11

3

42,35

3

Inspired by Life 2007 KRIBB Annual Report

DIVISION ofLEADING RESEARCH

Principle Functions and DutiesSystemic studies of protein structure and function

Development of NK cell therapy based on stem cell differentiationIntegrative research of BINT(bio/info/nano-technology) for healthcare

Comparative genome structure & genome network researchDiscovery of target and development of therapeutic antibodies for cancer therapy

Platform technology for metabolic syndrome

Systemic Proteomics Research Center <Stem Cell Research Center <

Bio-Nanotechnology Research Center <Genome Research Center <

Therapeutic Antibody Research Center <Metabolic Syndrome Research Center <

DIVISION ofLEADING RESEARCH2007 KRIBB Annual Report

19

ResearchersSeong Eon Ryu [email protected] Analysis of cellular switches

Hyun Jun Lee [email protected] Functional validation of drug targets

Jeong Hee Moon [email protected] Discovery of analytical techniques

Dae Gwin Jeong [email protected] Structure and function of PTPs

Tae Seong Yoon [email protected] Structure-based drug design

Research AreasAnalysis and applications of cellular switching- Analyzing the change in the proteome during the cellular damage

process caused by an ischemic stroke- Constructing a proteome signaling network for cellular switching- Uncovering disease target proteins

Research of protein structures and functions- Examining novel structures of protein tyrosine phosphatases (PTPs)

which are crucial to cell function- Researching the role of PTP proteins in a variety of organic

environments and discovering prospective disease targets, etc.

Development of analytical techniques and discoveringbiomarkers- Analyzing low amount proteins through mass-spectrometric

technologies- Discovering and verifying biomarkers

Selected PublicationsSeong Eon Ryu(Corresponding) Crystal structure of the majordiabetes autoantigen, IA-2 reveals distinctive immune epitopes Diabetes56: 41-48 (2007)Seong Eon Ryu(Corresponding) Broadly neutralizing anti-hepatitis B virus antibody reveals a complementarity determining regionH3 lid-opening mechanism Proc Natl Acad Sci U S A. 104: 9230-9235(2007)Seong Eon Ryu(Corresponding) Phosphoproteomic analysis ofneuronal cell death by glutamate induced oxidative stress Proteomics 7:2624-2635 (2007)Seong Eon Ryu(Corresponding) Structural insight into theconstitutive repression function of the nuclear receptor Rev-erb . J. Mol.Biol. 373: 735-744 (2007)Dae Gwin Jeong(First) Crystal structure of the catalytic domain ofhuman DUSP5, a dual specificity MAP kinase protein phosphataseProteins 66: 253-258 (2007) Jeong Hee Moon(First) Photodissociation at 193nm of some singlyprotonated peptides and proteins with m/z 2000-9000 using a tandemtime-of-flight mass spectrometry using glycerol: enhancement with adisperse laser beam Rapid Comm in Mass Spec. 21: 1468-1474 (2007)

AchievementsHigh-throughput discovery and validation of disease targetproteinsThrough subcellular fractionation and multi-dimensional mass analysismethods the proteome involved in brain cell death was analyzed, leadingto the discovery of target proteins for brain damage treatment and theestablishment of an activity analysis system for new target proteins.Regulatory substances were also identified.

Construction of a systemic research infrastructure for aprotein familyThe overexpression and purification of the PTP protein family wassuccessful. This result was used to clarify the tertiary structure of theprotein family, produce monoclonal antibodies and analyze systematicfunctions, all with a view to laying the foundations for a world-classcenter of excellence dedicated to researching PTP structures andfunctions.

Our goal is to develop platform technologies for disease treatmentand diagnosis through systemic proteomics studies of cellularfunctional switches. The major research area encompasses thestructural/functional analysis of disease related proteins and the high-throughput discovery of drug target proteins. Particular emphasis isplaced on the holistic and quantitative analysis of disease model cellsassociated with the vascular system.

Systemic ProteomicsResearch Center

Director Dr. Seong Eon RyuContact Tel +82-42-860-4149

Fax +82-42-860-4598E-mail [email protected]


21

ResearchersInpyo Choi [email protected] Differentiation of NK cells from hematopoietic stem cells- Anti-tumor NK cell therapy based on NK differentiation

In Seong Choe [email protected] Functional analysis of novel genes related to hepatoadenocarcinoma

Hee Gu Lee [email protected] Development of diagnostic techniques using cancer specific biomarkers

Jaewha Kim [email protected] Evaluation of NK cell effects on tumor suppression in animal models

Eun Young Song [email protected] Development of a cellular immunology-based diagnostic assay system

Kee Nyung Lee [email protected] Functional studies of VDUP-1 on tumorigenesis

Suk Ran Yoon [email protected] Regulation of NK differentiation from hematopoietic stem cells

Jin Woong Chung [email protected] Functional studies of the genes involved in NK differentiation

Research AreasCell differentiation- Developing platform technology for the differentiation of stem cells- Developing platform technology for the regulation of immune cell

activity

Cell therapy- Developing cell therapy for cancer treatment- Developing customized cell therapy through preclinical study

Selected PublicationsInpyo Choi(Corresponding) VDUP1 mediates nuclear export ofHIF1- via CRM1-dependent pathway BBA-MCR (in press) Inpyo Choi (Corresponding) Hypoxia-induced IL-18 increaseshypoxia-inducible factor-1 expression through a Rac1-dependent NF- Bpathway Mol. Biol. Cell (in press)Suk Ran Yoon, Jin woong Chung(First) and Inpyo Choi(corresponding) Development of Natural Killer Cells fromHematopoietic Stem Cells Mol. Cells 24(1): 1-8 (2007)Hee Gu Lee (Corresponding) Up-regulation of Mac-2 bindingprotein by hTERT in gastric cancer Int. J. Cancer 120(4): 813-20 (2007)Eun Young Song(Corresponding) Development of a rapid,immunochromatographic strip test for porcine rotavirus in stool J. Virologicalmethods (in press)Jae Wha Kim(Corresponding) S100A6 (Calcyclin) enhances thesensitivity to apoptosis via the up regulation of caspase-3 activity inHep3B cells J. Cellular Biochem. (in press)

AchievementsMolecular profiling for NK cell differentiation from stemcellsNK cells develop from hematopoietic stem cells (HSCs) in the bonemarrow. To understand the molecular regulation of NK cell development,serial analysis of gene expression (SAGE) was applied to HSCs, pNK,mature NK cells cultured without (-OP9) or with (+OP9) stromal cells,OP9. From 170,464 total individual tags from four SAGE libraries, 35,385unique genes were identified. The identification of genome-wide profilesof gene expression in different stages of NK cell development affords us afundamental basis for defining the molecular network during NK celldevelopment.

Molecular mechanism of VDUP1 during NK celldifferentiation from stem cellsIn order to observe the mechanism of VDUP1, the differentiation gene ofNK cells, knock-out mice for this gene were developed. Observation oftheir small intestines revealed abnormal proliferation and a 70% decreasein the number and activity of NK cells, leading to depressed anticanceractivity, and thus proving that this gene does in fact play a critical role inNK cell differentiation.

Development of immune therapy techniques utilizing NKcellsBased on the observations made during NK differentiation, animmunotherapy for cancer has been designed. Platform technology fordrug development for a cancer immune therapy was established and willbe used in the treatment of incurable diseases involving immune cells.

Our goal is to identify the differentiation factors involved in thedifferentiation of adult stem cells to immune cells, and by reserchingtheir functions, to develop the core platform technology for immune celltherapies for targeting cancer.

Stem Cell Research Center


Director Dr. In Pyo Choi Contact Tel +82-42-860-4223

Fax +82-42-860-4593 E-mail [email protected]

23

ResearchersBong Hyun Chung [email protected] Biochips and biosensors- Bionanomaterials

Haryoung Poo [email protected] Cellular immunology

Min-Gon Kim [email protected] Biosensors and biochips- Biochemical engineering

Yong-Beom Shin [email protected] material engineering for biosensors and biochips-BioMEMS

Sang Jeon Chung [email protected] Bioorganic and medicinal chemistry- Chemical biology

Yong Taik Lim [email protected] Bioimaging- Nanoparticles-based biophotonics

Moonil Kim [email protected] biology-Biochips and biosensors

Im Sik Chung [email protected] Biocompatible nanoparticles- Surface chemistry

Tae Hwan Ha [email protected] Nano-materials

Yongwon Jung [email protected] Biochemistry- Cancer biology

Chang-Soo Lee [email protected] Molecular self-assembly- Nanotechnology

Development of a mobile diagnostic sensor for liver diseaseA miniaturized strip sensor, which can measure the level of specificenzymes in the blood and thereby indicate the status of several liverdiseases, was developed and incorporated into a mobile cell-phonesystem. A prototype of this Mobile Lifecare System with developed stripsensors is shown in the Figure.

Selected PublicationsBong Hyun Chung(Corresponding) Wavelength and intensitymultiplexing of metal nanoparticles for the fabrication of multicoloredmicro- and nanospheres Advanced Functional Materials 16(8): 1015-102 (2007)Bong Hyun Chung(Corresponding) Surface plasmon resonanceimaging-based protein arrays for the high-throughput screening ofprotein-protein interaction inhibitors Proteomics 5(17): 4427-4431(2007)Bong Hyun Chung(Corresponding) Surface plasmon resonanceimaging protein arrays for the analysis of triple protein interactions ofHPV, E6, E6AP, and p53 Proteomics 6 (7): 2108-2111 (2007)Bong Hyun Chung(Corresponding) Oriented immobilization ofantibodies with GST-fused multiple Fc-specific B-domains on a goldsurface Analytical Chemistry 79(2): 546-556 (2007)Bong Hyun Chung(Corresponding) Direct immobilization ofprotein g variants with various numbers of cysteine residues on a goldsurface Analytical Chemistry 79(7): 2680-2687 (2007)Bong Hyun Chung(Corresponding) Self-directed and self-oriented immobilization of antibody by protein G-DNA conjugateAnalytical Chemistry 79(17): 6534-6541 (2007)

Research AreasProtein chips- Developing platform technologies to construct new generation protein

chips, whose detection system is free of fluorescence and radioisotopelabeling.

- Creating protein chips with biocontents that are employed in diseasediagnosis and high throughput screening of potential drug candidates.

NanobiomaterialsConjugating various biomolecular inorganic materials, such as metal ormagnetic nanoparticles, in order to investigate protein-proteininteractions and treat cancers.

NanobiosensorsDeveloping platform technology to establish and practically usebiosensors for the early diagnosis of diseases:- Label-free ultrasensitive nanobiosensing.- Interfacing biocontents with sensor substrates- Design and production of disease-specific biomarkers

Mobile lifecare systemDeveloping the necessary technology for the Mobile Lifecare System,incorporating IT with biochip and biosensor technology to enableportable health care.

AchievementsDevelopment of novel nanobiomaterials and technologytransferThe technology to produce metal nanoparticles capable ofaccommodating the visible spectrum of light and multicolor colloids wasdeveloped and used in the incorporation of gold and silver nanoparticlesfor pigment composition in cosmetics. This technology was transferred toNatural F&P Ltd. in January 2006.

Our research center is involved in the development of nanobiochips,nanobiosensors and nanobiomaterials through the utilization ofbiocontents. By integrating research into biotechnology (BT),nanotechnology (NT) and information technology (IT), we aim toconceive new drug discovery tools and technology for advanceddisease diagnosis and treatment, which will contribute to the creation ofnew businesses and realize our dream of enhancing and prolonginghuman life.

Bio-Nanotechnology Research Center

Director Dr. Bong Hyun ChungContact Tel +82-42-860-4442



25

ResearchersHong-Seog Park [email protected] Comparative analysis of gene structure between human and chimpanzee

brains- System development for the application of genomic sequence information- Unveiling the origins and migration of Koreans

Haeyoung Jeong [email protected] Analysis of microbial genome structure

Sang-Haeng Choi [email protected] Comparative analysis of gene structures between human and chimpanzee

brains- Genetic marker development in Korean cattle (Hanwoo)- Metagenome library construction from ancient DNA

Hyung-Cheol Kim [email protected] Molecular evolution of the non-coding region in mammals- Functional analysis of chimpanzee specific genes

Jungmin Seo [email protected] Development of an automated EST data analysis pipeline and system

Taeha Woo [email protected] Development of a database and web application for bioinformatics

Dae-Won Kim [email protected] Comparative analysis of gene structures between human and chimpanzee

brains- System development for the application of genomic sequence information

Genomic sequence variability of the prion gene (PRNP) inKorean cattleWe investigated sequence variants in the PRNP gene of 20 specimensbelonging to the Korean cattle breed, and have analyzed and comparedgenetic features between other varieties of cattle breeds. We discovered a2.6 kb SNP hot spot region localized in the putative promoter region ofthe PRNP gene. We also characterized a new 19 bp/10 bp allele located inthe putative promoter region.

Selected PublicationsHong-Seog Park(Co-author) Chromosomal localization of Koreancattle (Hanwoo) BAC clones via BAC end sequence analysis Asian AustralasianJournal of Animal Sciences 20(3): 316-327 (2007)Sang-Haeng Choi(First) & Hong-Seog Park(Corresponding)Genomic sequence variability of the prion gene (PRNP) in Korean cattleAsian-Australasian Journal of Animal Sciences 20(5): 653-660 (2007)

Research AreasAnimal genome- Comparative genome structure research into chimpanzees, whales, cattle,

and pigs.

Plant genome- High-throughput DNA sequencing of the Solanaceae sp. family of plants- Mass gene collection of industrially used plants, especially medicinal

plants grown domestically

Microbial genome- Meta-genome research of environmental organisms- Genome structure analysis based on massive genome sequencing of

various bacteria

Research support- Based on projects: governments and companies

AchievementsComparative analysis of the gene structures of chimpanzeeand human brainsOut of all the animals on earth, the chimpanzee most closely resemblesman. We constructed cDNA libraries of the chimpanzee brain (Cerebrumand Cerebellum), and sequenced 26,880 reads. We discovered 14,673unique genes in the cerebrum and 16,477 unique genes in the cerebellum.

Construction of comparative physical map between whalesand humansWe clearly explained that most parts of the OR cluster regions wereremoved from the false killer whale genome. Most of the remaining ORgenes turned out to be pseudogenes apart from some intact OR genes,which will provide a clue to understanding the evolutional mechanismof olfactory functions.

Our primary goal is to acquire an understanding of the biologicalphenomena of life through genomic science, which is a systematic andcomprehensive technology based on the genome information of variousorganisms. The Genome Research Center(GRC) has established a solidplatform for genomic sciences, and archived many world-leadingresearch products through international cooperation. Furthermore, weare actively collaborating with many academic and industry researchgroups in order to contribute to the advancement of Korea’s genometechnology industry.

Genome Research Center

Director Dr. Hong Seog ParkContact Tel +82-42-879-8132



27

ResearchersHyo Jeong Hong [email protected] Therapeutic antibody development- Biomarker discovery by antibody approach- Cancer biology

Youngwoo Park [email protected] Therapeutic antibody development - Target discoveries for a therapeutic antibody and a therapeutic receptor

Sang Seok Koh [email protected] Studies on targeted therapeutics through cancer genomics- Mechanistic investigation of novel metastatic factors

Jin-San Yoo [email protected] Development of antibody-based therapeutics- Engineering of antibody and beyond for clinical application

Semi Kim [email protected] Identification/validation of novel therapeutic targets in cancer

development and metastasis- Development of anti-cancer biologics drugs

Jeong-ki Min [email protected] Vascular biology- Biomarker discovery by antibody approach

Discovery of therapeutic targets for cancer therapyA Novel type II transmembrane serine protease was identified andcharacterized as a cancer therapeutic target. The new protease hasproven to be highly up-regulated in lung cancer tissues and is associatedwith the invasiveness, motility, and cell-matrix adhesion of cancer cells.The mining of a DNA microarray expression database also allowed us toidentify many novel genes with therapeutic potential. For example, thedevelopment of human antibodies targeting PAUF is underway fortherapeutic intervention.

Discovery of novel human embryonic stem cell (hESC)surface markersMonoclonal antibodies binding to the surface of undifferentiated hESCswere generated and selected. Their target antigens were identified andcharacterized. The mAbs will be useful in the identification andpurification of undifferentiated hESCs for basic research and clinicalapplication.

Selected PublicationsHyo Jeong Hong(Corresponding) Broadly neutralizing anti-hepatitis B virus antibody reveals a complementarity determining regionH3 lid-opening mechanism Pron. Natl. Acad Sci USA 104(22): 9230-59.6 (2007)Sang Seok Koh(Corresponding) Molecular cloning and functionalanalysis of a novel oncogene, cancer up-regulated gene 2 (CUG2)Biochem. Biophys. Res. Commun. 360(3): 633-9 2.9 (2007)Youngwoo Park, Semi Kim(Corresponding) TMPRSS4 promotesthe invasion, migration and metastasis of human tumor cells byfacilitating an epithelial-mesenchymal transition Oncogene (in press)

Research AreasCancer cell biology- Tumor progression and metastasis- Apoptosis - Intracellular signaling- Stem cells

Discovery and validation of drug targets for antibody-based cancer therapy- Discovery of drug targets through genomics and antibody approaches- Studies on the role of identified targets in tumor progression- Validation of the drug targets as anti-cancer agents

Development of therapeutic antibodies- Development of therapeutic antibodies such as human monoclonal

antibodies and humanized antibodies- Optimization of therapeutic antibodies through affinity maturation- Construction of a mammalian cell line producing therapeutic

antibodies or proteins- Production and purification of therapeutic antibodies or proteins- In vitro and in vivo study of therapeutic antibodies and proteins

AchievementsStructural analysis of the interaction between HBV neutralizinghumanized antibody and preS1 antigenA humanized antibody against the preS1 antigen of hepatitis B virus wasdeveloped. The complex structure of the antigen-binding pocket wasdetermined by crystallography and the functional mapping of the antigen-combining site was performed by alanine-scanning mutagenesis of all theCDRs. The information will be useful for a rational design regarding themaximal humanization and affinity maturation of the antibody.

Our goal is to discover new drug targets for antibody-based cancertherapy and to develop therapeutic antibodies and proteins. We alsostudy the molecular mechanism of cancer progression and validate theanti-cancer drug targets.

TherapeuticAntibody Research Center

Director Dr. Hyo Jeong HongContact Tel +82-42-860-4122



29

ResearchersYoung Ik Lee [email protected] Research and development of bioactive materials effective to NAFLD,

including fatty liver and liver cirrhosis

Sung Uk Kim [email protected] Research and development of bioactive materials including

antidiabetics and antifungal agents from natural resources

Tae-Sook Jeong [email protected] Research and development of bioactive materials including anti-

obesity and anti-atherosclerotic agents from natural resources andsynthetic compounds

Research AreasDevelopment of a platform technology for metabolicsyndrome- Investigation of the causes and discovery of targets for metabolic

syndrome- Studies of the regulation mechanism- Investigation of the genes and proteins related to metabolic syndrome

and their functional analysis

Development of bio-materials inhibiting metabolicfunction- Establishment of screening systems for regulators capable of controlling

metabolic syndrome- Research and development of novel therapeutic proteins and lead

compounds from natural resources and their derivatives, which havebiological effects on obesity, diabetes and cardiovascular diseases

- Evaluation of efficacy in animal models

Selected PublicationsYoung Ik Lee(Corresponding) Aberrant epigenetic modificationsin hepatocarcinogenesis induced by hepatitis B virus X-proteinGastroenterology 132: 1476-1494 (2007)Sung Uk Kim(Corresponding) Inhibition of chitin synthase 2 andantifungal activity of lignans from the stem bark of Lindera erythrocarpaPlanta Med. 73: 679-682 (2007)Tae-Sook Jeong(Corresponding) Effects of diarylheptanoids onthe tumor necrosis factor-a-induced expression of adhesion molecules inhuman umbilical vein endothelial cells J. Agri. Food Chem.(in press) Tae-Sook Jeong(Corresponding) Effects of 5-O-methylhirsutanonolon nuclear factor-kB-dependent production of NO and expression of iNOSin lipopolysaccharide-induced RAW264.7 Cells J. Agri. Food Chem.(in press)

AchievementsDevelopment of anti-atherogenic agents for the prevention/treatment of cardiovascular diseasesThe abietane compounds from Torreya nucifera leaves and new O-acyloxime derivatives were developed as anti-atherogenic agents. Inparticular, abietane compounds were observed to reduce atheroscleroticlesions and improve the inflammatory condition in cholesterol-fed LDLr-deficient mice. These active materials can be utilized for the developmentof nutraceuticals and new drugs for the prevention and treatment ofcardiovascular diseases.

Development of anti-fibrogenic agents for the treatmentof liver cirrhosisWe have developed an anti-fibrogenic which is effective in the treatmentof liver cirrhosis. We have almost finished the preclinical tests andtoxicological tests that are required for clinical applications. Aftersatisfying all the requirements we plan to carry out clinical tests at homeand abroad.

Research at the Metabolic Syndrome Research Center focuses onbasic and applied studies for the development of novel diagnostictechniques and therapeutic agents for metabolic syndrome. Theobjectives of such research activity are to discover the novel moleculartargets responsible for metabolic syndrome, and to search and developnovel therapeutic agents from natural resources, which are effective intreating obesity, diabetes, hyperlipidemia, and atherosclerosis, and thusto contribute to the promotion of a better quality of life and welfare.

MetabolicSyndrome Research Center

Director Dr. Young Ik LeeContact Tel +82-42-860-4150



DIVISION ofBIO- INTEGRATION RESEARCH

Principle Functions and DutiesResearching the function and clinical implications of the human disease genome

Researching disease model-based muromicsResearching regenerative medicine for embryonic stem cell therapy and xenotransplantation

Omics-based research for intelligent cellular engineering and molecular reconstruction Integrated and collaborative research of clinical and basic sciences

Medical Genomics Research Center <Disease Model Research Center <

Regenerative Medicine Research Center <Omics and Integration Research Center <

Translational Research Center <

2007 KRIBB Annual ReportDIVISION of

BIO-INTEGRATION RESEARCH

3332

target genes in order to define a therapeutically valid target for anti-cancer drug development in HCC.

Development of multi-purpose expression systems forstomach/liver cancer candidate genes Five hundred multi-purpose expression clones for the 100 candidategenes related to gastric or liver cancers were constructed using theGateway system. Since these clones can be expressed as His, GST or EGFPfusion proteins in E. coli, insect cells or mammalian cells, they can bedirectly used in a large-scale cell-based assay for a functional study aswell as in the large-scale preparation of antigen proteins for antibodyproduction.

Development of a high-content screening systemA high-content screening system using automated fluorescence imagingwas developed in order to identify candidate drugs for cancers includingliver and stomach cancers.

Construction of whole-genome mutant system of fissionyeast and application to anti-cancer drug developmentWe built a whole-genome deletion mutant library for the fission yeast S.pombe, and applied it to high-throughput gene function analysis anddrug screening. We identified one lead and several candidate compoundsfor anti-cancer drug development as well as novel genes involved in cellcycle regulation. A high-throughput platform for the analysis of themode of drug action is also being established, and together with thehigh-throughput drug screening system (HTS), collaborative researcheswith domestic pharmaceutical companies are currently underway.

Selected PublicationsSeon-Young Kim(Corresponding), In-Sun Chu (Corresponding)GAzer: gene set analyzer Bioinformatics 23(13): 1696-9 (2007)Yong Sung Kim(Corresponding) Epigenetic down-regulation andsuppressive role of DCBLD2 in gastric cancer cell proliferation andinvasion Molecular Cancer Research (in press)Kwang-Lae Hoe(Corresponding) PPARg activation abolishesLDL-induced proliferation of human aortic smooth muscle cells via SOD-mediated down-regulation of superoxide BBRC 359(4): 1017-23 (2007)Misun Won(Corresponding) Kyung-Sook Chung(First) Rapidscreening of human genes for their relevance to cancer using fissionyeast J. of Biomolecular Screening 12 (4): 568-577 (2007)Kyung-Sook Chung(First), Misun Won (First) Yeast-basedscreening to identify modulators of G-protein signaling usinguncontrolled cell division cycle by overexpression of Stm1 J. ofBiotechnology 129(3): 547-554 (2007)

Kyung Chan Park [email protected] Large-scale screening and identification of cancer-related genes- Functional validation of candidate target genes for therapeutics

development

Cho-Rok Jung [email protected] Functional analysis of genes associated with cancer- Development of anti-cancer gene therapeutic tools- Anti-cancer drug screening and validation

In-Sun Chu [email protected] Integrative Functional Genomics using microarray gene expression

profiling

Research Areas- Establishment of a functional genomics research infrastructure and

technology platforms- Large-scale screening and identification of disease-related genes- Functional validation of candidate target genes and biomarkers for

therapeutics/diagnostics development- Development of tools and strategies for modulating the therapeutic

targets and monitoring the biomarkers

AchievementsIdentification of E2 EPF UCP as a novel therapeutictarget of human cancers E2-EPF (UCP) associates with and targets pVHL for ubiquitin-mediatedproteolysis in cells, thereby stabilizing HIF-1 . UCP is detectedcoincidently with HIF-1 in human primary liver, colon and breasttumors, and metastastic cholangiocarcinoma and colon cancer cells.UCP promotes tumor growth and metastasis in vitro and mousexenograft models through effects on the pVHL-HIF pathway. UCP may bea new molecular target for therapeutic intervention in human cancers.

Development of therapeutic target genes for liver cancerAbout 2,500 hepatocellular carcinoma-associated genes were identifiedthrough DNA chip analysis of human liver cancer tissues. Theapplication of cell chip technology to these genes revealed 680 genes thatare functionally relevant to the regulation of major biochemicalpathways whose abnormality can lead to the development of liver cancer.From this candidate gene pool we finally identified 12 target genes withhigh therapeutic potential. Currently, we are extensively characterizing afew of them in terms of their biological function, as well as their clinicalsignificance in terms of cancer development and progression. Recently,we initiated a collaborative research project with Pfizer for three of these

ResearchersYoung Il Yeom [email protected] Genomic analysis of cancers and identification and functional

validation of therapeutic targets- Cancer gene therapy

Yong Sung Kim [email protected] Epigenomics in gastric and colon cancers - High-throughput LOH genotyping associated with gastric

cancer

Mi Sun Won [email protected] Functional validation of candidate target genes and

biomarkers for therapeutics/diagnostics development- Development of anticancer drugs by chemical screening and

study of modes of action

Kwang-Lae Hoe [email protected] Developing a drug target screening technology

Nam-Soon Kim [email protected] Identification and functional study of target genes related to

gastric and liver cancers- Systemic screening of human novel genes using the zebrafish

as an animal model

Kyung-Sook Chung [email protected] Development of anticancer drugs by chemical screening and

study of modes of action - Functional validation of candidate target genes for

therapeutics development

Young Joo Kim [email protected] Bioinformatics: Disease associated network analysis- Korean HapMap Database for Korean haplo-type mapping and

SNP statistical analyses

Dong-Uk Kim [email protected] Systematic parallel analysis of haploinsufficiency phenotype in S.pombe

- Drug target identification using drug-induced haploinsufficiency

Seon-Young Kim [email protected] Functional genomics approach to understanding human

cancers- Bioinformatics and computational biology

Soo Jung Kim [email protected] Functional studies on genes involved in liver cancer

Our goal is to establish top-quality genomics-based technologyplatforms and apply them to biomedical research programs in orderto achieve a high-throughput identification and global functionanalysis of the genes associated with the diseases that are mostprevalent in the Korean population such as stomach and livercancers. We also conduct functional genomics research to discovervalidated targets and biomarkers for the development of effectivediagnostics and therapeutics.

MedicalGenomicsResearch Center

Director Dr. Young Il YeomContact Tel +82-42-860-4109


DIVISION ofBIO-INTEGRATION RESEARCH

3534

Selected PublicationsDae-Yeul Yu(Corresponding) Hepatitis B Virus X Protein InducesHepatic Steatosis Via Transcriptional Activation of SREBP1 andPPARgamma Gastroenterology 132(5): 1955-1967 (2007) Dae-Yeul Yu(Corresponding) Roles of peroxiredoxin II in theregulation of proinflammatory responses to LPS and protection againstendotoxin-induced lethal shock JEM 204(3): 583-594 (2007)

AchievementsGeneration of an HBx transgenic mouse inducinghepatocellular carcinoma as well as hepatic steatosisThe results suggest that HBx may have multi-functions inducing notonly hepatocellular carcinoma but also hepatic steatosis, which cancause metabolic syndrome in HBV patients.

Generation of H-ras12V transgenic mice inducinghepatocellular carcinoma Gender-related differences in molecular responses to activated Ras mayhave implications for the prevalence of hepatic alterations in the mice.

Generation of Peroxiredoxin II knockout mouse withvarious phenotypes Peroxiredoxin II has been proven to be essential for sustaining the lifespan of erythrocytes, negatively regulating acute inflammation,preventing cellular senescence, regulating PDGF signaling and vascularremodeling, and regulating thymocyte maturations and proliferation.

Overexpression of the membrane-type matrixmetalloproteinase-1 gene induces mammary glandabnormalities and adenocarcinoma in transgenic miceMT1-MMP may have a tumorigenic function in mice.

ResearchersDae-Yeul Yu [email protected] Discovery of early detection biomarkers in cancer model mice- Discovery of new therapeutic targets by studying phenotypes

and in vivo function of disease model mice

Chul-Ho Lee [email protected] Development of animal models for human metabolic disease - Discovery and validation of the functional genes associated

with metabolic disease

Byung-Hwa Hyun [email protected] Genetic study of animal models for human neurological

diseases- Development of animal models for human diseases with non-

human primates

Research Areas- Discovery of early detection biomarkers in cancer model mice- Discovery of new therapeutic targets by studying phenotypes

and in vivo function of disease model mice- Development of techniques for the efficient production of

mutant mice, embryonic stem cells and MEF (mouseembryonic fibroblast) cells

- Research support with animal models

Our goal is to generate animal models for human diseases bymutagenesis such as transgenic, gene targeting and naturalbreeding, for in vivo validation of the genes associated with humandiseases. The establishment of an infrastructure for in vivo animalexperimentation is also an important objective of our center.

DiseaseModel Research Center

Director Dr. Dae-Yeul YuContact Tel +82-42-860-4422



3736

Selected PublicationsDeog-Bon Koo(Corresponding) Exogenous dibutyryl cAMP affectsmeiotic maturation via protein kinase A activation; it stimulates furtherembryonic development including blastocyst quality in pigsTheriogenology 69: 290-301 (2008)Deog-Bon Koo(First) Effects of daunorubicin on gangliosideexpression and neuronal differentiation of mouse embryonic stem cellsBiochem Biophys Res Commun. 362(2): 313-318 (2007)Yee Sook Cho(Corresponding) Microfabricated embryonic stemcell divider for large-scale propagation of human embryonic stem cellsLab Chip 7: 513-515 (2007)Yee Sook Cho(Corresponding) Simvastain suppresses self-renewal of mouse embryonic stem cells by inhibiting RhoAgeranylgeranylation Stem Cells 25: 1654-1663 (2007)Yong-Kook Kang(Corresponding) DNA methylation state ispreserved in the sperm-derived pronucleus of pig zygote Int. J. Dev. Biol.51(8): 707-14 (2007)Yong-Kook Kang(Corresponding) Dynamic DNA methylationReprogramming - Active De-methylation and Immediate Re-methylationin the Male Pronucleus of Bovine Zygotes Dev. Dyn. 236: 2523.2533 (2007)Yong-Kook Kang(Corresponding) Characterization of DNAmethylation change in stem cell marker genes during the differentiationof human embryonic stem cells Biochem. Biophy. Res. Comm. 359(3):536-542 (2007)

Research AreasStem cell - Developing platform technologies for the production of therapeutic

cells through mechanistic studies on stem cell self-renewal anddifferentiation

- Development of embryonic stem cell lines derived from interspeciessomatic cell nuclear transfer (iSCNT) blastocysts

Molecular control of early embryonic developmentStudying molecular and epigenetic mechanisms of early embryosdevelopment

OrganogenesisResearching the molecular development of organogenesis to gain a betterunderstanding of organ development

AchievementsStem cells- Signaling pathways important for ESC self-renewal and differentiation - Development of neural-lineage differentiation of human ESCs by

using an inhibitor of SHH signaling - Development of a large-scale culture system for hESCs using

nanotechnology

Molecular control of early embryonic development- Synchronizing meiotic resumption by dbcAMP treatment improves the

developmental capacity and embryonic qualities of IVF and SCNTporcine embryos

- In bovines, the compound processes of active DNA demethylation, and denovo DNA methylation, along with de novo H3-K9 trimethylation,alsotake place altogether within this very narrow window of pronucleusdevelopment

Organogenesis- The accumulation of reactive oxygen species (ROS) in the pancreas of

an adult cloned pig leads to apoptosis

ResearchersDeog-Bon Koo [email protected] Mammalian embryo development- Interspecies somatic cell nuclear transfer

Kyung-Kwang Lee [email protected] Xenotransplantation- Production of knock-out clone pig

Yong-Kook Kang [email protected] Epigenetic control of early mammalian development- HTS Development for drug screening in human ES cells

Kweon Yu [email protected] Molecular genetics

Yee Sook Cho [email protected] Stem cell-based drug screening- Signaling mechanisms controlling ESC self-renewal anddifferentiation

Janghwan Kim [email protected] Differentiation of human ESCs toward neural stem cells.- Stem cell-based drug screening for neural and cardiac cells.

Jung Sun Park [email protected] Nuclear transfer for the production of cloning animals,- Micromanipulation of mouse, porcine and bovine oocytes

Sun Mi Woo [email protected] Signaling mechanisms controlling neural stem cell self-

renewal and differentiation

Mi-Young Son [email protected] Cardiomyocyte differentiation from human embryonic stem

cells

Our goal is to develop platform technologies for regenerative biologythrough fundamental studies on development and differentiation inhumans and model organisms.

RegenerativeMedicine Research Center

Director Dr. Deog-Bon KooContact Tel +82-42-860-4418



3938

Development of glyco-engineered yeast to producehuman mannose-type glycoproteinsThe glycosylation pathways of non-traditional yeasts, H. polymorphaand Yarrowia lipolytica, have been elucidated and reconstructed in orderto produce human-compatible mannose-type glycoproteins, which willbe used as high quality and economical hosts for the mass production ofhigh-value added therapeutic glycoproteins.

Visualization and quantification of in vivo signaling dynamicsWe developed a protocol to detect in vivo interactions between proteinpartners. We can obtain the live cell imaging of fluorescence-probedsignaling molecules after expression in a mammalian cell line.

Selected PublicationsHyun Ah Kang(Corresponding) Identification of the cadmium-inducible Hansenula polymorpha SEO1 gene promoter by transcriptomeanalysis and its application to whole-cell heavy-metal detection systemApplied and Environmental Microbiology 73 (19): 5990-6000 (2007)Hyun Ah Kang(Corresponding) Engineering of the yeast Yarrowialipolytica for the production of glycoproteins lacking the outer-chainmannose residues of N-glycans Applied and EnvironmentalMicrobiology 73 (14): 4446-4454 (2007)Ki-Sun Kwon(Corresponding) Cytoplasmic localization andubiquitination of p21(Cip1) by reactive oxygen species Biochem BiophysRes Commun. 358 (22): 219-225 (2007)Ki-Sun Kwon(Corresponding) Curcumin attenuates glutamate-induced HT22 cell death by suppressing map kinase signaling Mol CellBiochem. 298(1-2): 187-194 (2007)Sung Sup Park(2nd author) In a model of immunoglobulinheavy-chain (Igh)/c-myc translocation, the Igh 3 regulatory regioninduces c-myc expression at the immature stage of B cell developmentGene Chromosome Canc. 46(10): 950-959 (2007)

Research AreasOmics-based cellular modeling and reconstruction- Integrated genomic analysis of the metabolic regulatory networks and

stress response mechanisms - Functional genomic analysis and the application of protein secretion

and modification networks- Development of synthetic regulatory circuits and signal transduction

networks based on Omics information

Cellular kinetics of signal transduction proteins- Study of genetic regulation and biochemical characterization of signal

transduction proteins- Study of spatio-temporal kinetics of signaling proteins by using bio-

imaging technology- Modeling of the signal transduction process based on the integrated

analysis and reconstruction of the movement and interaction of proteincomplex

Glycomics-based glycan remodeling and fusion technology Development of a glyco-engineered strain library by glycosylation pathwayreconstruction- Development of a glycan modifying enzyme library and an enzymatic

glycan modification process- Development of a high-throughput and ultra-sensitive glycan analysis

system - Development of a glycan-based bio-chip and cellular imaging technology

AchievementsDevelopment of integrated genomics platforms forindustrial yeast, Hansenula polymorphaWe have established platforms for proteomics and glycomics research ofmethylotrophic yeast, H. polymorpha, along with the development ofwhole-genome cDNA microarrays. This will facilitate an IntegratedOmics approach to obtain solid knowledge about the cellular systems ofH. polymorpha at the global level, thereby enabling the successfulexploitation of the industrial potential of this yeast.

ResearchersHyun Ah Kang [email protected] Integrated genomics and cellular engineering of yeast- Protein secretion and glycosylation

Ki Sun Kwon [email protected] Cellular kinetics of signal transduction protein - Bio-imaging of in vivo signaling dynamics

Myung Kyu Lee [email protected] Ab generation against post-translationally modified protein - Peptide biochemistry and biology

Seong Sup Park [email protected] Neuronal cell death mechanisms- Apoptotic signaling in cancer cells

Ohsuk Kwon [email protected] Functional genomics and synthetic biology of prokaryotes - Signal transduction and molecular genetic engineering

Doo-Byoung Oh [email protected] Development of glycan remodeling technology- Protein therapeutics and engineering

Our goal is to develop core platform technology that will be useful forthe production of next-generation bio-therapeutics and high-valueadded metabolites. We focus on intelligent cellular engineering andmolecular reconstruction based on the understanding of lifephenomena at system level via the acquisition and integratedanalysis of various Omics data, such as functional genomics,proteomics and glycomics.

Omics andIntegration Research Center

Director Dr. Hyun Ah KangContact Tel +82-42-860-4378



4140

Proteomic research on neuronal cell deathProteomic research led to the discovery of key protein players in theapoptosis of neuronal cells. Functional studies on these proteins wereconducted.

Research on apoptosis and cell signalingProteomic research led to the discovery of regulators of cellular apoptosis andcell signaling. The results were published in the major scientific journals.

Selected PublicationsDo Hee Lee & Byoung Chul Park(Co-corresponding) Proteomicanalysis of glutamate-induced toxicity in HT22 cells Proteomics 7(2):185-193 (2007)Byoung Chul Park(Corresponding) G1 to S phase transitionprotein 1 induces apoptosis signal-regulating kinase 1 activation bydissociating 14-3-3 from ASK1 Oncogene (in press)Kwang-Hee Bae(First) & Sang Chul Lee(Corresponding)Phosphoproteomic analysis of neuronal cell death by glutamate-inducedoxidative stress Proteomics 7(15): 2624-2635 (2007)Do Hee Lee & Sung Goo Park(Co-corresponding) Caspase-7mediated cleavage of proteasome subunits during apoptosis Biochem.Biophys. Res. Comm. 363(2): 388-394 (2007)Eui-Jeon Woo(Corresponding) Structural insight into theconstitutive repression function of the nuclear receptor Rev-erbbeta J.Mol. Biol. 373(3): 735-744 (2007)Kwang-Hee Bae & Sung Goo Park (Co-corresponding) Gpx3of S. cerevisiae suppresses non-enzymatic degradation of glutaminesynthetase in an activity-independent manner Biochem. Biophys. Res.Comm. 362(2): 405-409 (2007)

Research AreasAutoimmune disorderDiscovery and functional verification of biomarkers from patientssuffering immune diseases, such as atopic dermatitis, asthma, andrheumatoid arthritis

ApoptosisIdentification and functional studies on new substrate of caspases, keyregulators of apoptosis

Neuroscience and neurodegenerative diseasesProteomic research into neuronal cell functions and neurodegenerativediseases

Differentiation of mesenchymal stem cellsDiscovery and functional verification of the genes and marker proteins,that are involved in the differentiation of human mesenchymal stemcells into adipocytes and osteoblasts

Cell signalingResearch on the mechanisms of key cell signaling pathways, e.g. theMAPK pathway

Phathogenic microbesProteomic research into pathogenic microbes (e.g. Vibrio) and industrialmicrobes

AchievementsProteomic research on pathogenic microbes, VibrioProteomic research on pathogenic microbes, Vibrio, led to the discoveryof proteins, which were implicated in Vibrio virulence. Functional studiesof these proteins were conducted.

ResearchersByoung Chul Park [email protected] Target mining and validation using proteomics- Translational research of immune diseases

Sang Chul Lee [email protected] Elucidation of reaction mechanism of TCDD - Proteomic analysis of neuronla cell death pathway and stem

cell differentiation

Sung Goo Park [email protected] Mechanism and functions of apoptosis-related proteins- Protease degradomics

Do Hee Lee [email protected] Functions and regulation of ubiquitin-proteasome system- Protein misfolding diseases (e.g., Huntington s disease, ALS, SCA)

Seung Jun Kim [email protected] Structural studies on anti-oxidant proteins and protein tyrosine

phosphatases- Drug development using 3-D structural information

Eui-Jeon Woo [email protected] Structural and functional studies on DNase and proteins in

apoptosis- Hormone nuclear receptors and their application

Sunghyun Kang [email protected] Aptamers- Proteomics and mass spectrometry

Kwang-Hee Bae [email protected] Target mining and validation using reverse genetics tools- Proteomic analysis of neuronal cell death pathway and stem

cell differentiation

Jae-Ran Lee [email protected] Functional study of neuronal synapse formation by protein

tyrosine phosphatases- Regulation of synaptogenesis by microglia in the brain

We are set to establish ourselves as the R&D hub of nationwidetranslational research in Korea. We are establishing closecollaboration with many partner groups in basic research andclinical medicine. Our major research interests include immunediseases, apoptosis, degenerative neuro-diseases, stem celldifferentiation, and cell signaling.

TranslationalResearch Center

Director Dr. Byoung Chul ParkContact Tel +82-42-860-4260



DIVISION ofMOLECULAR THERAPEUTICS

Principle Functions and DutiesIdentification of therapeutic targets in cancer and discovery of their modulators

Development of new drug candidates from natural resourcesDiscovery of bioactive metabolite from microorganisms and plants

Molecular Cancer Research Center <Natural Medicines Research Center <

Functional Metabolite Research Center <


MOLECULAR THERAPEUTICS

4544

Research Areas- Target identification and validation by chemical method and NMR

technology- Discovery of anti-tumor agents from natural sources and peptides- Lead identification/optimization for development of novel anticancer

therapeutics- Mode of action of candidate compounds using genomic or proteomic tools

AchievementsFinding STAT3 inhibitor from natural productsSugiol, a diterpene compound, was identified from the screening ofnatural products using the STAT3-dependent luciferase reporter system.We confirmed that sugiol specifically inhibits only Tyr705 of STAT3 inDU145 prostate cells and that the expression level of cyclin D1, cyclin Aand survivin is down-regulated by STAT3 activity.

Development of a small molecule inhibitor of HIF-1 activityStructural modification of a hit compound discovered during high-throughput screening using an HRE-dependent reporter assay hasrevealed a novel class of HIF-1 inhibitors, which potently inhibit the HIF-1a protein accumulation and its target gene expression under hypoxicconditions in human hepatocellular carcinoma Hep3B cells.

Validation of DRG9 in an animal model as an oncogeneA novel protein, DRG9, has been identified via the elucidation of amolecular mechanism of a p50 selective inhibitor, a natural diterpenecompound. This protein, which is induced by a variety of kB activators,potently stimulates kB target gene expression, p65/RelA transcriptionalactivity, and HIF-1a accumulation. Furthermore, over-expression ofDRG9 in cancer cells significantly increased its anchorage independentgrowth, invasiveness, and angiogenic activity, suggesting that DRG9could be an oncogenic protein.

Determination of a structural basis for the broad neutralizationof an HBV-neutralizing antibody We have determined the crystal structures of the humanized monoclonalantibody HzKR127 Fab and its complex with preS1 epitope peptide of thehepatitis B virus. In the complex structure, the bound peptide forms atype IV -turn followed by a 310 helical turn, whose looped-outconformation provides a structural basis for broad neutralization.

ResearchersByoung-Mog Kwon [email protected] Chemical genomics using cell- or phenotype-based assay, gene and

protein expression profiling- Natural product chemistry

Jung Joon Lee [email protected] Discovery and optimization of anti-cancer drug-lead from chemical

library and natural products targeted NF-kB, HIF-1, and PPAR andevaluation of their efficacy in animal models

- Molecular pharmacology and target discovery of bioactive naturalproducts

Kyou-Hoon Han [email protected] Study of natively unstructured proteins by NMR structural biology- Development of peptide drugs using -conotoxins

Kwang-Hee Son [email protected] Study of microbial natural products as SMDs (small molecular drugs)- Study of microbial enzymes as a protein drug

Young-Soo Hong [email protected] Manipulation of antibiotic biosynthetic genes to make precise structural

changes in a complex antibiotics molecule- Heterologous expression of an artificial biosynthetic pathway

Dong Cho Han [email protected] Study of cancer migration and metastasis, and development of its

blocking chemicals - Study of apoptosis induced by cell cycle arrest and stress

Seung-Wook Chi [email protected] Structural biology of p53 in the cancer protein network - Structure-based drug development

Kyeong Lee [email protected] Medicinal chemistry and chemical biology of a small molecule inhibitor

of HIF (Hypoxia Inducible Factor) as a potential anticancer agent- Development of ansamycin analogues as a potent HSP90 inhibitor

Selected PublicationsSeung-Wook Chi(First) Broadly neutralizing the anti-HBVantibody revealed a CDR H3 lid opening mechanism Proc. Natl.Acad. Sci. USA 104: 9230-9235 (2007)Kyeong Lee(First) & Jung Joon Lee(Corresponding)(Aryloxyacetylamino)benzoic acid analogues: A new class ofhypoxia-inducible factor-1 inhibitor J. Med. Chem. 50: 1675-1684 (2007)Kwon, B, M.(Corresponding) Apoptosis induction of 2 -hydroxycinnamaldehyde as a proteasome inhibitor is associatedwith ER stress and mitochondrial perturbation in cancer cellsBiochem. Pharm. 74: 557-565 (2007)Jung Joon Lee & Young-Soo Hong(Corresponding)Mutasynthesis of geldanamycin by the disruption of a geneproducing starter unit: generation of structural diversity in thebenzoquinone ring ChemBioChem 8: 1491-1494 (2007)Kyou-Hoon Han(Corresponding) Pre-structured motifsin the natively unstructured preS1 surface antigen of thehepatitis B virus Protein Science 16: 2108-2117 (2007)Kyeong Lee & Jung Joon Lee(Corresponding)Synthesis of (aryloxyacetylamino)-isonicotinic/nicotinic acidanalogues as a potent hypoxia-inducible factor (HIF)-1alphainhibitor Bioorganic Med. Chem. Lett. 1997, 17(22): 6305-6310 (2007)

The goals of the molecular cancer research center are to discovernovel molecular targets for anticancer drug screening and todevelop anti-cancer drug candidates against the novel targets. Weapply functional and chemical genomics in order to identify the geneor protein involved in tumor progression and metastasis. To findnovel anti-cancer agents, we screen chemical library and naturalproducts using cell-based assay systems.

MolecularCancerResearch Center

Director Dr. Byoung Mog KwonContact Tel +82-42-860-4557



4746

Research AreasIdentification of new molecular targets related to immuneand metabolic diseases- Identifying major genes or proteins involved in the immune system and

lipid metabolism, and their functional analysis- Establishing a bioassay/screening system for such compounds, and

developing specifically active compounds

Immunomodulator research- Researching the cellular response modulator affected by the excessive

activation of immune cells- Researching the inhibition activity of respiratory viruses- Isolating natural bioactive compounds and synthesizing them, and

developing a potent compound by structure optimization

Metabolome research- Isolating metabolomes derived from plants - Elucidating metabolome structure and researching its metabolism

Construction of a natural product library- Collecting plant resources domestically and abroad, and securing data- Constructing a plant extract library and a natural compound library

Establishment of a wild plant seed bank in Korea- Collecting native plant seeds and making voucher specimens- Constructing a database system for seeds and the study of seed properties

AchievementsIdentification of disease-oriented molecular targetcandidatesThrough research of molecular targets for asthmatic diseases, threedisease target candidates have been identified. This enabled thedevelopment of new anti-asthmatic drug candidates, which are beingprepared for preclinical testing (of toxicity, PK, etc.).

Identification of leads and candidates for new drugsDiscovery research for chronic inflammatory diseases including asthma,allergies and cancer and metabolic disorders such as obesity and diabetesrevealed about 20 leads and 10 therapeutic candidates, includingverproside.

Development of anti-viral agentsIn the search for natural anti-viral agents, a material showingexceptional activity against white-spot syndrome virus was discovered.Research for industrialization is ongoing.

ResearchersHyeong-Kyu Lee [email protected] Identification of new molecular targets related to immune diseases,

establishment of a bioassay/screening system for such compounds, anddevelopment of active compounds

- Construction of a natural product library

Kyung-Seop Ahn [email protected] Determination of anti-inflammatory and anti-allergic activity of

natural products using a mouse asthma model and cell-based assay

Sei-Ryang Oh [email protected] Identification of biologically active compounds from natural sources- Establishment of a screening system for anti-asthmatic & anti-

inflammatory activity.

Ho-Jae Lee [email protected] Development of an assay system for the screening of TGF- signal

modulators- Molecular mechanisms of TGF- signaling during inflammation

and/or airway remodeling

Sangku Lee [email protected] Organic synthesis of natural compounds and medicinal chemistry for

the development of drug candidates

Joongku Lee [email protected] Establishment of a wild plant seed bank to ensure the ex-situ

conservation of plant resources and to develop mass production for highvalue-added products

Hyun Sun Lee [email protected] Development of therapeutic agents/nutraceuticals for metabolic diseases

including obesity, type 2 diabetes, insulin resistance, hyperlipidemia andconstruction of bioactive substances from natural products

Young-Kook Kim [email protected] Establishing a screening system for metabolic diseases including

atherosclerosis and obesity, and developing specifically bioactivecompounds

Mun-Chual Rho [email protected] Identification of inflammation related target molecules - Eestablishment of screening systems and development of active compounds

for inflammation related diseases

Dur-Han Kwon [email protected] Identification of cultures and a quantitative titration method for several

viruses- Development of new active compounds against diseases induced by

viruses including the influenza virus, rotavirus, coranvirus, rhinovirusand enterovirus

Construction of biomaterial infrastructureWith regard to natural product library construction, we arecontinuing to expand the number of samples in our plant extractand seed bank libraries (plant extracts: 500; natural products: 50;seed bank: 500 a year), and have launched a promotionalcampaign to encourage their use.

Industrial researchWe developed natural anti-bronchitis agents and were able toregister a patent and exchange information with severalcompanies.

Commercialization of cholesterol-lowering drugsWe developed cholesterol-lowering agents from a natural sourceand transferred them to Dong-Hwa Pharm.Ind.Co. Ltd for anti-atherosclerosis drugs.

Selected PublicationsHyeong-Kyu Lee & Kyung-Seop Ahn(Corresponding)Anti-inflammatory and anti-allergic effects of kefir in a mouseasthma model Immunobiology 212(8): 647-654 (2007)Hyeong-Kyu Lee & Sei-Ryang Oh(Corresponding)Suppressive effect of verproside isolated from Pseudolysimachionlongifolium on airway inflammation in a mouse model ofallergic asthma Int. Immunopharmacology 6 (6): 978-986(2007)Ho-Jae Lee(Corresponding) Negative regulation ofActivin/Nodal signaling by SRF during Xenopus gastrulationDevelopment 134: 769-777 (2007)Ho-Jae Lee(First) SRF is a nuclear repressor of Smad3-mediated TGF- signaling Oncogene 26: 173-185 (2007)Mun-Chual Rho(Corresponding) Dimericsesquiterpenoids isolated from Chloranthus japonicus inhibitedthe expression of cell adhesion molecules J. Ethnopharmacol.104(1-2):270-7 (2007)Hyun Sun Lee(Corresponding) Inhibition ofdiacylglycerol acyltransferase by alkamides isolated from thefruits of Piper longum and Piper nigrum J. Agric Food Chem.54(26):9759-9763 (2007) Hyun Sun Lee(Corresponding) Inhibition ofdiacylglycerol acyltransferase by betulinic acid from Alnushirsuta Planta Med. 72 (3): 267-269 (2007)

Our aim is to develop natural therapeutics that will be effectiveagainst chronic immune diseases (e.g. asthma, arthritis, vasculardiseases, metabolic diseases, etc.) which are difficult to treat withmodern drugs, from the natural product library, starting withtraditional medicines.

NaturalMedicinesResearch Center

Director Dr. Hyeong Kyu LeeContact Tel +82-42-860-4413



4948

Antimicrobial agentsDiscovering novel antibiotics against drug resistant pathogenic bacteria,and determining their mode of action

Epigenomic modulatorsDeveloping bioactive compounds for regulating DNA methyltransferase-mediated gene expression and carcinogenesis

AchievementsApoptotic death of prostate tumor by a compound isolatedfrom plant extractsFive compounds showing similarities in their chemical structure wereisolated from a plant extract. Among them, four had a strong induction ofapoptosis, inducing the activation of caspase-3 and PARP cleavage.Exploitation of the mechanism of the compounds indicated that FLIP,downstream of Fas, was down-regulated in its expression by thecompounds. The malignant growth of cancer cells, including HBx-transformed liver and K-ras-transformed prostate cells, was also reduced bythe compounds. Furthermore, one of the compounds significantly reducedtumor size in a mouse model injected with the K-ras-transformed cancercells. The related patents and manuscripts are under preparation.

Isolation of ER-stress inducer and inhibitorAn endoplasmic reticulum (ER)-stress inducer was isolated from a plantextract. This compound strongly induced the expression of ER-stress-associated proteins, including GRP78 chaperone, and the splicing of XBP-1mRNA, a hallmark of ER-stress-induced IRE-1a activation. Conversely, aninhibitor of ER-stress was also isolated for use as an inhibitor of type IIdiabetes mellitus. Both are expected to be effective in cancer therapeuticsand anti-diabetes.

Isolation and application of extremophilesOver twenty new species of microorganisms, including the halophilicHalomonas kribbensis were isolated from extreme environments such assalt fields. The metabolite analysis and study of these organisms arecurrently taking place.

ResearchersJong-Seog Ahn [email protected] Microbiology and cell biology- Antidibetes and anticancer agents

Bo-Yeon Kim [email protected] Biochemistry and cell biology- Anticancer and antidiabetics

Chang Jin Kim [email protected] Microbiology- Microbial diversity and biocontrol agent

Jong-Pyung Kim [email protected] Natural product chemistry- Antioxidants and cosmeceuticals

Won-Gon Kim [email protected] Natural product chemistry- Antibiotics

Ick-Dong Yoo [email protected] Natural product chemistry- Cosmeceuticals

Bong-Sik Yun [email protected] Natural product chemistry- Mushroom metabolite

Research AreasObesity/DiabetesDeveloping anti-obesity and anti-diabetic lead compounds with regulatoryroles in the insulin singnaling and ER-stress induced gene expression

Natrural product library Isolate and deposit the bioactive secondary metabolites through microbialsynthetic biotechnology and metabolomic technology

AntioxidantsDeveloping new bioactive antioxidant compounds from mushrooms andother natural materials

Information exchange and commercialization ofobesity/diabetes therapeuticsPurified compounds from Torreya nucifera, Salviamiltiorrhiza, Morus sp were identified as having anti-obesity andanti-diabetic effects through the inhibition of proteindephosphorylation, leading to increased insulin sensitivity. Theyproved to be effective in the mouse model system. This technologyhas been exchanged with a corporation and research is underwayfor its use in pharmaceutical drugs and functional productscontaining insulin sensitizing compounds.

Selected PublicationsJong Seog Ahn(Corresponding) NF-kB inhibitionenhances caspase-3 degradation of Akt1 and apoptosis tocamptothecin Cell Signaling 19: 1713-1721 (2007)Bo Yeon Kim(Corresponding) NF-kB inhibition increaseschemosensitibity to trichostatin A-induced cell death of Ki-Ras-transformed human prostate epithelial cells Carcinogenesis 27:2258-2268 (2007)Won-Gon Kim(Corresponding) Fumimycin: a PeptideDeformylase Inhibitor with an Unusual Skeleton Produced byAspergillus fumisynnematus Org. Lett. 9 (13): 2449-2451(2007) Chang-Jin Kim(Corresponding) Shimazuella kribbensisgen. nov., sp. nov., a mesophilic representative of the familyThermoactinomy-cetaceae Int. J. Syst Evol Microbiol 57(11):2660-2664 (2007)Ick-Dong Yoo(Corresponding) Glucose starved HT-29human colon calcinoma cells are sensitive to verrusosidin as aGRP78 down-regulator Toxicology 229(3): 253-261 (2007)Jong-Pyung Kim(Corresponding) Cyathusals A, B and C,New Antioxidants from the Fermented Mushroom Cyathusstercoreus J. Nat. Prod. 70(6): 1043-1045 (2007)Bong-Sik Yun(Corresponding) Highly oxygenated andunsaturated metabolites providing a diversity of hispidin classantioxidants in the medicinal mushrooms Inonotus andPhellinus Bioorg. Med. Chem. 15: 3309-3314 (2007)

The goal of the research center is to discover the bioactivemetabolites of microorganisms and plants in order to find drugcandidates for metabolic and infectious diseases through screeningand development. In order to accomplish this goal, we havedeveloped a screening system based on cell biology andmetabolomics and constructed a metabolite bank isolated frommicroorganisms and plants to develop medicinal and functional well-being compounds.

FunctionalMetabolite Research Center

Director Dr. Jong seong AhnContact Tel +82-42-860-4312



DIVISION ofBIO-MATERIALS SCIENCE

Principle Functions and DutiesStructural and functional genomics of economically important plants

Genome-based systems microbiologyEnvironmental biotechnology to produce sustainable industrial materials

Plant Genome Research Center <Systems Microbiology Research Center <

Environmental Biotechnology Research Center <


BIO-MATERIALS SCIENCE

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Development of an edible plant-derived vaccine forAlzheimer s diseaseAntigens for mutant -amyloid proteins, which are known to be acause of Alzheimer s disease, were overexpressed in potatoes as anedible vaccine. We have developed the transgenic potato, and haveconfirmed through experiments with mice that the potato vaccine doesproduce specific antibodies for -amyloids. We need to conductadditional animal trials, as well as tests for toxicity, before we can usethis product as a human vaccine.

Selected PublicationsMin SR(First) A sepal-expressed ADP-glucose pyrophosphorylasegene (NtAGP) is required for petal expansion growth in 'Xanthi' tobaccoPlant Physiol. 145 (1): 277-289 (2007)Youm JW(First) & Kim HS(Corresponding) Oralimmunogenicity of potato-derived HBsAg middle protein in BALB/cmice Vaccine 25: 577-584 (2007)Park JM(Corresponding) The chili pepper CaATL1: an AT-hookmotif-containing transcription factor implicated in defence responsesagainst pathogens Mol. Plant Pathol 8(6): 761-771 (2007)Hur CG(Corresponding) TISA: Tissue-specific alternative splicingin human and mouse genes DNA Research 13(5): 229-243 (2007)

AchievementsInternational collaboration for analysis of the SolanaceaegenomeThe Plant Genome Research Center supervises the internationalconsortium-driven research for the genomic analysis of Solanaceae, andparticipates in the international tomato genome sequencing project. Thisten-year project, launched in 2004, involves 20 nations. Ten countries areparticipating in primary operations to decode the genomic sequence.Korea is responsible for the 2nd chromosome, which consists of 12% ofthe entire genome; thus far we have accomplished about 60% of ourdesignated task. We hope that this may serve as an opportunity tointernationally promote our country’s plant bioengineering industry.(http://sol.pdrc.re.kr)

Large-scale isolation of pepper genes and public releaseWe have undertaken the task of gene identification in peppers, Korea smost important vegetable product, and have finished analyzing about120,000 expressed sequence tags. A database for this information hasbeen developed and opened to the public. We believe that we have securedmore than two-thirds of the pepper genome, which consists of 30,000unigenes, and expect varieties information to be helpful in thedevelopment of new strains. (http://sol.pdrc.re.kr)

Development of platform technology for plant functionalgenomics researchVirus-induced gene silencing was developed for the large-scale screeningof genes. This technology is being used in the screening of many types ofSolanaceae plants including Nicotiana benthamiana, peppers, andtomatoes. The resultant data are expected to be widely used in theidentification of functional genes.

Development of transplastomic technology The need to improve transgenic technology in order to prevent therandom release of artificially produced genes into the environment viathe pollen of transgenic plants is gaining prominence. We developedtransformation technology for foreign gene expression in plastids. Thesegenes can only be inherited from the maternal line, which cannot bespread through pollen, thereby ensuring a low environmental risk.

ResearchersJang Ryol Liu [email protected] Won Joong Jeong [email protected] Ran Min [email protected] - Development of CyanoCrops by introducing cyanobacterial genes

into the chloroplast genome of crops- Cloning of salt resistance genes from marine cyanobacterias via a

functional genomics approach and development of salt-tolerant crops- Development of new cultivars of strawberry by genetic transformation

Jae Heung Jeon [email protected] Soon Kim [email protected] Development of an edible vaccine for Alzheimer s disease- Development of transgenic crops - Development of novel binary vectors for molecular farming

Suk Yoon Kwon [email protected] Goo Hur [email protected] Solanaceae international genome project - Development of a gene pool involving tissue-specific and alternative

splicing- Plant, microbial genome databases and annotation

Jae Sun Moon [email protected] Sun Cho [email protected] Mee Park [email protected] Molecular plant-microbe interactions- Development of an oligo DNA chip for the diagnosis of pathogens- Identification of the genes involved in development by virus-induced

gene silencing

Research Areas- Research of plant genome structure- Research into the functional genomics of plant-microbe interactions- Research into bio-informatics technology and establishment of a

platform for various genomes- Research into the development of an environmentally-friendly binary

vector system- Research into the signal transduction network of plant cell death - Research into CyanoCrops by using cyanobacterial genes

The center has focused on the development of the platform technologyrequired for the industrial application of functionally important genesand new transgenic plants with useful traits. We have conductedresearch of the structure and functional genomics of economicallyimportant plants of the Solanaceae family (e.g. peppers, tomatoes,potatoes), and have secured successful results and platformtechnologies for their commercial application.

PlantGenome Research Center

Director Dr. Suk Yoon KwonContact Tel +82-42-860-4340



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Continued discovery of bacterial biodiversity - maintainingNo 1. ranking in the field - Isolation of 5 new genus of bacteria and more than 30 novel species- Isolation of novel class lipase from metagenome library

Selected PublicationsYoon, S. H., Park, Y.-K., Lee, S., Choi, D., Oh, T. K., Hur, C.-G.,and Kim. J. F. Towards pathogenomics: A web-based resource forpathogenicity islands Nucleic Acids Res. 35: D395-D400 (2007)Ha, J.S., Song, J.J., Lee, Y.M., Kim, S.J., Sohn, J.H., Shin, C.S.,and Lee, S.G. Design and application of highly responsivefluorescence resonance energy transfer biosensors for the detection ofsugars in living Saccharomyces cerevisiae. Appl. Envir. Microbiol.73(22): 7408-7414 (2007) Cho, H.-S., Park, S.-Y., Ryu, C.-M., Kim. J. F., Kim, J.-G., andPark. S.-H. Interference of quorum sensing and virulence of the ricepathogen Burkholderia glumae by an engineered endophytic bacteriumFEMS Microbiol. Ecol. 60: 1-14 (2007)Kwon, S. J., Jung, H. C., and Pan, J.G. Transgalactosylation in awater-solvent biphasic reaction system with -galactosidase displayed on thesurfaces of Bacillus subtilis spores Appl. Envir. Microbiol. 73: 2251-2256(2007)Yoon, J.-H., Kang, S.-J., Jung, S.-Y., and Oh, T.-K. Humicoccus flavidusgen. nov., sp. nov., isolated from soil. Int. J. Syst. Evol. Microbiol.57: 56-59 (2007)

Research AreasMicrobial genome analysis Genome sequencing and functional genomics of industrialmicroorganisms ; deciphering microbial diversity on a genome scale

Microbial cell factoryDeveloping novel expression systems with yeast and bacteria, metabolicpathway engineering, and engineered bacteria-plant interaction

Biocatalyst innovationCustom-made enzymes, biomolecular engineering, and innovativebiocatalysis processes

AchievementsConstruction of a yeast protein factory for the efficientproduction of recombinant proteins for therapeutics andindustrial enzymes - Genome-wide screening of the TFP library- Efficient secretion of difficult-to-express proteins and enzymes

Dissecting probiotic Paenibacillus-plant interactions andtheir genome analysis- Understanding Paenibacillus-elicited plant growth promotion and

induced resistance - Functional analysis of biosynthetic genes of polymyxin and fusaricidin - Improvement of crop productivity by plant-associated Paenibacillus spp.

Development of biocatalytic processes - Biodiesel production with immobilized and improved lipase- Whole-cell biocatalysis with surface-displayed -galactosidase

Genome analysis of Escherichia coli B and microbialpathogenicity islands - Genome analysis of E. coli BL21(DE3), REL606, and evolved strains - Comparative omics analysis of E. coli B and K-12: transcriptomes to

phenomes- Web service of a database for prokaryotic pathogenicity islands

ResearchersJae Gu Pan [email protected] Microbial physiology, bioprocessing

Dae Sil Lee [email protected] Carbohydrate enzymes, antimicrobials

Eui Sung Choi [email protected] Yeast expression system, metabolic engineering

Seung Hwan Park [email protected] Bacillus genetics, GRAS microbial factory

Jung Hoon Sohn [email protected] Yeast protein factory, yeast genetics

Seung Goo Lee [email protected] Biomolecular engineering, bioimaging, biosensors

Jihyun F. Kim [email protected] Microbial genomics, bacterial pathogenicity

Jung Hoon Yoon [email protected] Bacteria taxonomy, microbial biodiversity

Heung Chae Jung [email protected] Protein engineering, biocatalysis

Soo Keun Choi [email protected] Bacillus genetics, metabolic regulation

Choong-Min Ryu [email protected] Bacteria-plant interactions, plant immunity

Myung Hee Kim [email protected] Protein structure and functions

Sung Ho Yoon [email protected] Metabolic engineering, bioinformatics

Our aim is to become a national competence center specializing inmicrobial biotechnology by developing the platform technology requiredfor microbial genomics and microbial cell factories.

SystemsMicrobiologyResearch Center

Director Dr. Jae Gu PanContact Tel +82-42-860-4483



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Microbial community researchDeveloping functional microbial communities for bioremediation ofcontaminated soil and monitoring microbial diversity and functions

AchievementsDevelopment of plants with enhanced tolerance to multipleenvironmental stressesTransgenic crops (e.g. sweetpotato, potato) for sustainable agricultureand environments were developed through the gene manipulation ofantioxidative mechanisms under the control of an oxidative-stressinducible SWPA2 promoter. These plants showed a strong tolerance tomultiple environmental stresses, including paraquat-mediated oxidativestress, drought, extreme temperatures and air pollution. In addition,root-specific promoters are under study for the production of bioenergyand functional feed materials in transgenic sweetpotatoes.

Greenhouse gas reduction and beneficial materialproductionOutdoor mass bioreactor and cyanobacteria harvesting apparatus weresuccessfully developed; they showed improved efficiency in carbondioxide fixation. Thus, the produced cyanobacterial biomass could beused in health foods, cosmetics, and medicines. In addition,environmentally-friendly materials and apparatus were developed forbloom control.

Development of functional microbial communities forbioremediationSeveral functional microbial communities (FMCs) degrading highlypetroleum oil were obtained from oil-contaminated soil throughsequencing enrichment techniques. Their microbial diversity wasexamined using DGGE and t-RFLP. We developed an effective FMCpreservation technology using solid carriers and filtration. After 6 monthsof preservation, FMC maintained the degrading activity and microbialdiversity by more than 90%.

ResearchersSang-Soo Kwak [email protected] Transgenic plants with enhanced tolerance to multiple environmental

stresses - Molecular breeding of sweetpotato for sustainable development in

marginal lands

Hee-Mock Oh [email protected] Ecophysiological study of microalgae and cyanobacteria in eutrophic

waters- Production of high-value materials from microalgae and cyanobacteria

Haeng-Soon Lee [email protected] Transgenic plants with enhanced tolerance to multiple environmental

stresses- Transgenic plants for bioenergy and functional feed materials

Hee-Sik Kim [email protected] Biodegradation of petroleum hydrocarbons in contaminated soil by a

microbial community- Molecular analysis of microbial diversity and functions in

contaminated environments (chlorinated compounds, ECDs, heavymetals)

Chi-Yong Ahn [email protected] Ecological modeling for freshwater quality and cyanobacterial bloom- Ecophysiological and molecular study of cyanobacteria and their toxins

Research AreasPlant antioxidation researchDeveloping industrial transgenic plants with enhanced tolerance tomultiple environmental stresses for sustainable agriculture andenvironments in marginal lands

Microalgae researchUsing cyanobacteria in carbon dioxide reduction and developingenvironmentally-friendly production technology for useful materials

Director Dr. Sang Soo KwakContact Tel +82-42-860-4432


Selected PublicationsSang-Soo Kwak(Corresponding) Enhanced tolerance oftransgenic sweetpotato plants expressing both superoxide dismutase andascorbate peroxidase in chloroplasts against methyl viologen-mediatedoxidative stress Mol. Breeding 19: 227-239 (2007)Hee-Mock Oh(Corresponding) Determination of cyanobacterialdiversity during algal blooms in Daechung reservoir, Korea, on the basisof cpcBA intergenic spacer region analysis App.l Environ. Microbiol.72: 3252-3258 (2007)Hee-Mock Oh(Corresponding) Life cycle of the ichthyotoxicdinoflagellate Cochlodinium polykrikoides in Korean coastal watersHarmful Algae 6: 104-111 (2007)Hee-Sik Kim(Corresponding) Cloning of srfA operon fromBacillus subtilis C9 and its expression in E. coli. Appl. MicrobiolBiotechnol. 75: 567-572 (2007)Chi-Yong Ahn(First author) Alternative alert system forcyanobacterial bloom, using phycocyanin as a level determinant J.Microbiol. 45: 98-104 (2007)

We aim to develop industrial platform technology using high-techecogenomics and biological resources in response to the UnitedNations Framework Convention on Climate Change for sustainabledevelopment. To achieve this goal, we focus on the development ofintegrated fusion technology combined with plant science, microbialscience including microalgae, and environmentally-friendly materialsscience.

EnvironmentalBiotechnologyResearch Center


DIVISION ofBIOTECHNOLOGYR&BDIn order to promote and commercialize our research outcomes, we are focusing on bioprocess development for thecommercialization, technology transfer and licensing in/out, and the effective management of the intellectual properties ofKRIBB. In particular, we are carrying out a process scale-up, optimization and industrialization through pilot plant facilities,cultivation of human resources, and academic-industrial collaboration especially for the commercialization of bio-products.Additionally, we are systematically registering and managing the intellectual properties of KRIBB, as well as engaging intechnology transfer and supporting the business activities of bio-companies.

ResearchersJoon-Ki Jung [email protected] Genetic engineering- Bioprocess engineering

Hong-Weon Lee [email protected] Fermentation physiology- Bioprocess engineering

Eun-Gyo Lee [email protected] Recombinant protein productionand purification

- Cell culture

Jung-Oh Ahn [email protected] Genetic engineering- Metabolic engineering

Research AreasBioprocess development for the production of bioactive materials andbiopharmaceuticalsProcess development, process optimization, and scale-up of the entire bioprocess includingexpression system, fermentation, downstream process and chromatographic purification

Microbial fermentation and animal cell cultureHigh quality and high yield production of valuable metabolites and recombinant proteins bybatch, fed-batch and continuous cultures

Providing pilot plant facilities Providing diverse pilot plant facilities for the prototype production and R&D collaboration forthe manufacturing process development

Patent management and technology transferManagement of KRIBB's patents, and the promotion of technology transfer and licensingin/out

Venture promotion and innovative supportOne-roof business-support system ranging from the incubation of bioventure companies tomanagement and marketing consultation

AchievementsProduction of a pronase from Streptomyces griseusIn order to produce a pronase (mixture of proteolytic enzymes) cost-effectively, we screened the appropriate mutant strains after consideringproductivity, growth rate and stability. We then optimized the fermentationconditions including culture environment and media. In a pilot-scalefermentor (2,500 liters) the pronase was stably produced up to 3,600 U/mL.We are currently preparing to license this technology to a bio company.

Development of recombinant vaccines for livestockLeptospiral outer membrane proteins (OMPs) have been known to play animportant role in the pathogenicity of Leptospira. New vaccine strategiesusing the OMPs as subunit-antigens are deemed necessary for effectiveprotection against Leptospirosis instead of the classical vaccines based oninactivated whole cells. We developed an expression system of recombinantLipL41 to enhance the expression of their antigenic subunits effectively andevaluated the potential of recombinant LipL41 as a subunit-antigen toprotect against Leptospirosis. Also, the processes for the mass-productionand purification of this protein were developed, while supportive tests onthe immunogenicity of the purified protein were conducted on guinea pigs.

Production of shikimic acid in metabolically-engineeredEscherichia coliShikimic acid is a high-value compound that serves as a key startingmaterial for Tamiflu, for use as an orally active antiviral compound for theprevention and treatment of influenza infections. An alternative to theextraction of shikimic acid from Illicium plant is fermentative productionby metabolically engineered microorganisms. For the fermentativeproduction of shikimic acid, we developed rationally designed E. colistrains by blocking the aromatic amino acid pathway after the productionof shikimic acid. Furthermore, we developed the fermentation process foran effective production of metabolically-engineered E. coli.

Production of bioactive human cytokines in recombinantEscherichia coli One of the major issues in the bioindustry and proteomics research area isto find a solution that will enable us to produce bioactive recombinanthuman proteins by using the functional fusion proteins and signal human

proteins by using the functional fusion proteins and signal sequences inE.coli. We developed the secretion technology of human cytokines for theirmass-production and were able to optimize the purification process toproduce pure and high yields.

Production of therapeutic proteins in mammalian cellcultureMammalian cell culture has become the dominant system for theproduction of recombinant proteins for clinical applications because oftheir proper protein folding and complete post-translationalmodification. We are developing rCHO cell culture technology for thelarge-scale production of therapeutic proteins in a suspension bioreactor,and the boosting technology for high-quality proteins by geneticallyengineered cells has also been studied. Furthermore, chromatographicpurification and high-throughput analysis technology have beendeveloped.

Selected PublicationsJung-Oh Ahn(First) & Hong-Weon Lee(Corresponding)Translation elongation factor 1- gene from Pichia pastoris: molecularcloning, sequence, and use of its promoter Appl. Microbiol. Biotechnol.(2007)Joon-Ki Jung(Corresponding) Over-production of beta-carotenefrom metabolically engineered Escherichia coli. Biotechnol. Lett.28(12):897-904 (2007)Joon-Ki Jung(Corresponding) Enhanced protease cleavageefficiency on the glucagon-fused interleukin-2 by the addition ofsynthetic oligopeptides Protein Expr. Purif. 55(1):159-65.d (2007)

DIVISION ofBIOTECHNOLOGY R&BD

Director Dr. Joon Ki Jung Contact Tel +82-42-860-4510


DIVISION of Ochang R&D Bio-EvaluationCenter Our aim is to establish a specific and collective foundation for the techniques, facilities, and manpower required to support theeffective and successful development of biotech products. For this purpose, we have not only constructed an evaluationinfrastructure for optimizing, analyzing, and standardizing living modified organisms, drug candidates and lab animals, but wehave also been assessing the usefulness and risks posed by biotech products and biotech R & D processes so as to helpfacilitate their commercialization.

ResearchersHwan Mook Kim [email protected] Risk assessment of LMOs- Evaluation of anticancer and immunomodulating drug candidates

Stephen Beungtae Ryu [email protected] Human and environmental risk assessment of LMOs- Identification of commercially valuable genes/LMOs

Won Kee Yoon [email protected] Veterinary pathology- Health safety assessment of LMOs

Soon-Chun Jeong [email protected] Genetics of plant disease resistance genes- Molecular genetics characterization of LMOs

Chang-Gi Kim [email protected] Plant and fungal ecology- Environmental risk assessment of LMOs

Hyoung-Chin Kim [email protected] Toxicology- Germ-free animal production

Ki-Hoan Nam [email protected] Experimental animal medicine- Mouse stem cells

Young-Suk Won [email protected] Microbiology- Health monitoring of laboratory animals

Song-Kyu Park [email protected] Molecular pharmacology- Evaluation of anticancer drug candidates

Kiho Lee [email protected] Biopharmaceutics- Drug metabolism and pharmacokinetics in drug discovery

Jong Soon Kang [email protected] Molecular pharmacology- Efficacy evaluation of immunomodulatory and anticancer agents

DIVISION ofOchang R&D

61

Research AreasLiving modified organisms (LMOs)Conducting a genetic analysis of LMOs as well as assessing the risksposed by LMOs to humans and the environment. Identification ofcommercially valuable genes and LMOs

Drug discoveryDiscovery and preclinical evaluation of new drug candidates

Laboratory animalsActing as a national center for lab animal standardization, developingtransgenic animals and evaluating their usefulness

AchievementsLiving modified organismsWe have established and developed infrastructures for the geneticanalysis and assessment of the potential risks posed to humans and theenvironment by living modified organisms. Notably, we moved tonewly built greenhouse and laboratory facilities at Ochang,Chungbuk, at the end of 2006. In addition, on the basis of thisinfrastructure, we have been assessing the potential risks ofdomestically developed transgenic rice, chilli peppers, potatoes, poplarsand rootstocks for watermelons. We have also conducted NationalEnvironmental Monitoring on domestic soybeans, corns and oilseedrapes and inspected the extent of their genetic contamination byimported LMOs. In collaboration with academic and private institutes,we have identified commercially valuable LMOs.

Drug discoveryDrug discovery and development is a highly valuable area includingtarget identification/validation, hit identification, lead optimization,and preclinical/clinical studies. We have developed and implementedan integrated infrastructure for drug discovery that encompassespreclinical efficacy, ADME and toxicity evaluation. Furthermore, wehave applied this technology platform to the discovery and preclinicalevaluation of drug candidates, such as anticancer agents, celltherapeutics, antirheumatics and antiallergics, for cancer and

immunological diseases. Our infrastructure for drug discovery plays akey role in providing practical support to many drug discovery effortsin the fields of academia and industry.

Experimental laboratory animalsLaboratory animals are essential in biosciences and biotechnologies.Our role involves the collection, maintenance, standardization anddevelopment of laboratory animals to make them invaluable publicresources. We have also been carrying out environmental, genetic andhealth monitoring to ensure the safe, reliable and reproduciblescientific usages of laboratory animals. As a national laboratoryanimal resources center, the total number of laboratory animals wehave collected amounts to 201 strains. We have distributed animalresources to 27 organizations and performed health-monitoring for2,099 heads of 30 organizations. We are also developing an in vivoshort/mid-term carcinogenesis assessment system using mice for newdrug candidates.

Selected Publications Soon-Chun Jeong (Corresponding) & Hwan Mook Kim(Corresponding) Molecular analysis and quantitative detection of atransgenic rice line expressing a bifunctional fusion TPSP FoodControl 18: 1434-1442 (2007)Hwan Mook Kim (First) & Song-Kyu Park (Corresponding)Structure-activity relationship studies of a series of novel d-lactam-based histone deacetylase inhibitors Journal of Medicinal Chemistry50: 2737-2741 (2007)Hwan Mook Kim (First) Anti-tumor activity of ex vivo expandedcytokine-induced killer cells against human hepatocellular carcinomaInternational Immunopharmacology 7: 1793-1801 (2007)Jong Soon Kang (First) & Hwan Mook Kim (Corresponding) Topicalapplication of a novel ceramide derivative, K6PC-9, inhibits dust miteextract-induced atopic dermatitis-like skin lesions in NC/Nga miceInternational Immunopharmacology 7: 1589-1597 (2007)Young-Suk Won (First) Helicobacter callitrichis sp. nov., a novelHelicobacter species isolated from the feces of the common marmoset.(Callithrix jacchus) FEMS Microbiology Letters 271: 239-244 (2007)

Director Dr. Hwan Mook KimContact Tel +82-42-240-6520


DIVISION of Ochang R&D National PrimateResearch CenterThe NPRC was established in the KRIBB as a major national infrastructure with the purposes of supportingindustrial/academic/research institutions in their pursuit of developing xenotransplant organs, providing animal models forresearch into regenerative medicine and incurable diseases, and evaluating the preclinical trials of new drug candidates.

ResearchersKyu-Tae Chang [email protected] Developing cells and tissue resources derived from non-human primates

and researching their applications- Developing new breeder miniature pigs for the research and

development of bio-organs

Ekyune Kim [email protected] Study on the functional relationship of membrane proteins involved

in mammalian fertilization- Down-regulation of membrane proteins through the endocytosis

mechanism

Sang-Hyun Kim [email protected] Development of specific detection methods for pathogen

identifications from animal samples- Structural/functional studies of lipopolysaccharides (Salmonella,

Yersinia spp.)

Research AreasAcquisition/propagation/distribution of specificpathogen free (SPF) primate resourcesAcquiring and propagating SPF primate resources, and thendistributing them to industrial/academic/research institutions

Standardization in handling and regulating labrequirements for primate researchMaintaining quality in the standard of primate resources bymonitoring bacteria, viruses and general health parametersEstablishing a standard operating procedure (SOP) for providingguidelines for the breeding and management of primate resources atthe international level

Xenotransplantation researchTransplanting organs (e.g. pancreatic islet, heart) from transgenicgerm-free pigs into SPF primates and analyzing the efficacy and safetyof the transplanted organs

DIVISION ofOchang R&D

63

Regenerative medical research and applicationsUsing primate disease models in cell therapy and gene therapyresearch, and evaluating their efficacy/safety for the treatment ofincurable diseases

Preclinical efficacy assessment of newly-developeddrug candidatesApplying various biodrugs and biomaterials to SPF primates in orderto evaluate their efficacy

Evaluation of the immunogenicity and safety of vaccinecandidatesTesting various vaccines and AIDS vaccines and assessing theirimmunogenicity, efficacy and safety

Development of disease modelsConstructing disease models for incurable diseases from primates,which have the most similar metabolic pathways to mankind, andthus developing new drugs and applications for organ xenograft andregenerative medicine

Collaboration/support for nationwide non-institutionalresearch involving primatesProviding specialized technology and information about primate careand facilities to other researchers, and conducting collaborativeresearch for the development of related technologies

AchievementsAcquisition of primate resourcesPrimates give us valuable opportunities in providing the non-humanphysiological/anatomical data required for biomedical research andits applications. The NPRC currently houses six types of SPF primates:Rhesus monkeys, Cynomolgus monkeys, African green monkeys,Japanese monkeys, squirrel monkeys, and the common marmoset,which gives a total of 110 animals.

Transfer of primate-related resources and techniquesto national partners at industrial/academic/researchinstitutionsThe NPRC shares its primate-related expertise with researchersnationwide, in fields such as neuroscience, pharmacokinetics, and soforth. We provide services for the upkeep of SPF primates, includingmicrobiological monitoring, quarantine and maintenance workshops,and also train the personnel (e.g. veterinarians and breeders) whowork with the primates.

Collaboration with national and international researchteamsWe have conducted collaborative studies in various fields, includingxenotransplantation and the pharmacokinetic evaluation oftherapeutic drugs against aplastic anemia. For the introduction ofbaboons, we cooperated with the Washington, Tulane, and SouthwestNational Primate Research Centers, and are currently collaboratingwith globally renowned researchers in embryo implantation anddevelopment. We are also working together with domestic companiesfor the development of mini-pigs, which are useful in the generationof xenotransplantational organs.

Selected Publications Sang-Hyun Kim(Co-author) Secondary acylation of Klebsiellapneumoniae lipopolysaccharide contributes to sensitivity toantibacterial peptides Journal of Biological Chemistry 282:15569-15577 (2007) Sang-Hyun Kim(Co-author) Contribution of AIDA-I to thepathogenicity of a porcine diarrheagenic Escherichia coli and tointestinal colonization through biofilm formation in pigs VeterinaryMicrobiology 120: 308-319 (2007)

Director Dr. Kyu Tae ChangContact Tel +82-43-240-6300


Jeonbuk Branch InstituteMolecularBioprocessResearch CenterOur goal is to develop biotechnologies and bioprocesses for the production of microbial metabolites, proteins, industrialenzymes and bioenergy, all of which are useful for the pharmaceutic, nutraceutic, dietetic, cosmetic, feed and fine chemicalindustries, etc.

ResearchersChul Ho Kim [email protected] Development of the human papillomavirus vaccine- Bioconversion of by-products generated by the palm oil industry

Hyo Kon Chun [email protected] Process development for the production of a flexible display substrate- Process development for the production of a Tamiflu substrate from

quinic acid

Joong Su Kim [email protected] Study and genetic analysis of carbohydrate polymer producing genes

in Aureobacidium species- Development of the glycosyltransferases applicable to industries

Jae Jun Song [email protected] Development of platform technology for massive screening and the

commercialization of industrially valuable enzymes using the HTS system- Development of hyper-sensitive cell-based genetic circuitry for the

screening of functional genes from meta-genomic DNA

Sukhoon Koh [email protected] Development of genetic tools for Aureobasidium sp.- Development of enzymes for the carbohydrate industry

Woo Song Lee [email protected] Development of atherogenic agents from various plants and

microorganisms- Development of a trans-sialidase inhibitor from various plants and

microorganisms

Jeong-Woo Seo [email protected] Development of processes for the production of highly pure

polyunsaturated fatty acids- Development of biorefinery technologies

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Research AreasMicrobial metabolic engineering- Production of microbial metabolites- Metabolic engineering of industrial microorganisms

Bioconversion technology- High throughput screening of novel biocatalysts- Directed evolution of industrial enzymes- Production of bio-substances by bioconversion

Molecular bioprocess engineering- Production of therapeutic recombinant proteins- Development of bio-refinery technologies

AchievementsXanthones from Cudrania tricuspidata displayingpotent -glucosidase inhibitionWe have proven that xanthone compounds isolated from the root of C.tricuspidata possess highly potent -glucosidase inhibition properties.

Novel Levansucrase enzymeNovel levansucrase was isolated from Bacillus megaterium and wascloned in E. coli.

Development of human papillomavirus vaccineHuman papilomavirus genes coding L1 protein were isolated fromKorean patients infected with various types of HPV.

Selected Publications Jeong-Woo Seo(Coauthor) Thermolysis of iso-migrastatin andits congeners via [3,3]-sigmatropic rearrangement: a new route to thesynthesis of migrastatin and its analogues Organic Letters 8(25):5865-5868 (2007)Woo Song Lee(Corresponding) Human ACAT inhibitory effectsof shikonin derivatives from lithospermum erythrorhizon Bioorg.Med. Chem. Lett. 17: 1112-1116 (2007)

Director Chul Ho KimContact Tel +82-63-570-5110


Jeonbuk Branch InstituteBioindustry Research CenterOur goal is to develop the technology required for the mass-production of microbial metabolites and industrial enzymes.Additional objectives of the center include the construction of a base for regional bio-industry development through technicalsupport business for the activation of agriculture and stockbreeding, and research into mass-production with regard to theindustrialization of useful bio-materials.

ResearchersByung Dae Yoon [email protected] Base construction for regional bio-industry development through

research into microbial-materials for agriculture and stockbreeding- Development of a mass-production process and analysis of immune

activity of -glucan purified from Aureobasidium sp.

Min Soo Kim [email protected] Development of fermentation techniques of biopolymers- Bioconversion of a high-intensive sweetener from waste orange peel

Research AreasDevelopment of a mass-production technology for useful biologicalcompounds and research into practical technology through field application- Search for new functional biological compounds- Development of mass-production technology for useful biological compoundsusing pilot plants

- Development of field application technology for the commercialization ofuseful compounds

Achievements Environmentally-friendly agriculture based on biologicalcontrol technologyAgriculture in the next decade will have to produce more food from lessland through the more efficient and sustainable use of natural resourcesand inflict minimal impact on the environment in order to meet growingpopulation demands. Promoting and adopting "environmentally-friendly agriculture by biological control technology and itsmanagement systems" could help us to reach this goal. Thus, we arefocusing on conservation agriculture, defined as mineral disturbancecombined with microorganisms in soils, as a more sustainablecultivation system for the future.

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Application of immunostimulators for non-antibiotic pig-breeding

-Glucan, an endogenous polysaccharide immunostimulator, exhibitshigh adjuvant activity in domestic breeding animals. It is one of the non-toxic, non-pyrogenic, and water-soluble immunostimulators. We havediscovered that -glucan has an immunoenhancing effect on pigs as anadjuvant and as a nonspecific immunostimulant. As such, furtherinvestigations in isolating and characterizing new biological agents asimmunomodulators should continue in view of this success.

Search for industrially useful microbial resources fromKorean traditional fermented foodsWe have studied the functional effects of Korean-style fermented foodssuch as kimchi, soy sauce, etc. Traditional Korean fermented foodspromote good digestion, because the consumption of a cup of clear soupcontaining soy sauce enhances gastric juice secretion in humans.Fermented soy sauce contains three tartaric isoflavone derivatives calledsoy flavones. These soy flavones were shown to have inhibitory activitiesagainst histidine decarboxylase, which produces histamine, a mediator ofinflammation, allergy and gastric acid secretion. Soy sauce also exhibitsantiplatelet activity. Beta-Carbolines were isolated from soy sauce as theactive compounds. Soybeans and wheat, which are the main rawingredients of soy sauce, are allergenic foods.

Development of a high-intensity sweetener using citruspeel waste- Recently, a high-intensity sweetener(a low-calorie, full-bulk naturalsugar) has just attained GRAS (Generally Recognized As Safe) statusunder the U.S. Food and Drug Administration (FDA) regulations, therebypermitting its use as a sweetener in foods and beverages. We have studiedthe development of NHDC produced from citrus peel waste, with regard toits demonstrated food and beverage applications and the potential healthand medical benefits of this unique substance. NHDC has been found tobe safe and efficacious for use as a low-calorie, full-bulk sweetener in awide variety of foods, beverages, health foods, and dietary supplements. Itfills broad, heretofore unmet needs for a low-calorie sweetener inproducts in which the bulk of sugar is important, such as chocolates,chewing gum, cakes, ice cream, and frosted cereals. Its synergism withother sweeteners also makes it useful in various foods. The various healthand medical benefits of NHDC have been indicated, including thetreatment of type 2 diabetes, hyperglycemia, anemia, and hemophilia, aswell as the improvement of fetal development.

Selected Publications Byung-Dae Yoon(Corresponding) Extracellular production of aglycolipid biosurfactant, mannosylerythritol lipid, by Candida sp. SY16using fed-batch fermentation Applied Microbiology and Biotechnology70(4 ): 391 - 396 (2007)

Director Byung Dae YoonContact Tel +82-63-570-5210


BIOTECHNOLOGY R&DINFRASTRUCTURE> Biological Resource Center> Daejeon-KRIBB-FHCRC Resource Cooperation Center> Korea Biosafety Clearing House> Biotech Policy Research Center> KOrean BioInformation Center(KOBIC)> International Biological Material Research > The 21st Century Frontier R&BD Program> International Cooperation> Technology Commercialization> Support for Technology Information

BIOTECHNOLOGY R&D INFRASTRUCTURE 2007 KRIBB Annual Report

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Biological Resource CenterAs a national bio-infrastructure for biological resources, we perform the role of a biotechnology think-tank and bio R&D. Themain goal of the BRC, as a national infrastructure, is the collection, preservation and distribution of biological resources.

ResearchersHee-Mock Oh [email protected] Ecophysiological study and mass cultivation of microalgae and

cyanobacteria

Ho-Yong Park [email protected] Insect pathology and microbiology for biomaterials and bioactive

substances

Kyung Sook Bae [email protected] Microbial taxanomy

Jung-Sook Lee [email protected] Management of bacteria and national bio R&D products

Chang-Bae kim [email protected] Research on biodiversity information and DNA barcode records

Suk Weon Kim [email protected] Management of plant cell lines

Kee-Sun Shin [email protected] Management of yeast and fungi

Young-Hyo Chang [email protected] Management of anaerobes and research into anaerobes and probiotic

bacteria

Song-Gun Kim [email protected] Management of plasmid resources

Jin-Woo Bae [email protected] Management of archaea

Hyun Woo Oh [email protected] Structural biology and insect histology

Doo Sang Park [email protected] Microbiology and microbial enzymology

Research AreasBiological resource acquisitionCollection and preservation of core biological resources, both foreignand domestic, offering public support by distributing biologicalresources to academia, industry, and research institutions, andorganizing patent strain deposits

Development of core technologies for valuable bioresourcesDeveloping platform technology for the identification and preservationof useful biological resources, the constant screening of new species,the screening of highly active enzymes (e.g. proteinase, xylanase,lipase) and bioactive substances derived from insects, insect-relatedmicroorganisms and various environments, and using them for thedevelopment of nutraceuticals and biomaterials

Construction of an information network and support ofvarious servicesBuilding local and international information networks for biologicalresources and providing support for workshops, conferences, andconsultation, etc.

AchievementsCollection and preservation of biological resourcesOver 1,300 strains, including bacteria, actinomycetes, yeasts, filamentousfungi, anaerobes, cell lines and patent strains, were acquired, and about63,000 cases of long-term preservation were recorded.

Research activitiesWe published 56 papers concerning biological resources, and described22 new species. We also generated and registered 17 patents, includingone international patent. We developed a management system forbiological resources using barcodes (IRIS, Information of Resource-Indexing System, v3.0) for the implementation of systematic and efficient

management. We also improved industrial microbes and developedproduction processes for the efficient manufacture of the highly activeproteinase, Arazyme, from a Korean spider. Highly active xylanase andlipase were also developed from insects. These technologies weretransferred to a related corporation for industrialization.

Biological resource distribution and information-sharingOver 4,000 strains were distributed to academia, industry, and researchinstitutions. We held four workshops and two conferences, and offeredconsultation and technical support for more than 7,000 cases.

Selected PublicationsSong-Gun Kim(First) A direct substrate-substrate interactionfound in the kinase domain of the bifunctional enzyme, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase J. Mol. Biol. 370:14-26 (2007)Jin Woo Bae(Corresponding) Natronococcus jeotgali sp. nov.,a halophilic archaeon isolated from shrimp jeotgal, a traditionalfermented seafood from Korea Int. J. Syst. Evol. Microbiol. 57: 2129-2131 (2007)Jin Woo Bae(Corresponding) Vibrio litoralis sp. nov., isolatedfrom a Yellow Sea tidal flat in Korea Int. J. Syst. Evol. Microbiol. 57:562-565 (2007)Jin Woo Bae(Corresponding) Marinobacterium halophilumsp. nov., a marine bacterium isolated from the Yellow Sea Int. J. Syst.Evol. Microbiol. 57: 77-80 (2007)

Director Dr. Hee-Mock OhContact Tel +82-42-860-4600


BiologicalResource Center

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Daejeon-KRIBB-FHCRC ResearchCooperation CenterOur goal is to discover cancer biomarkers of high sensitivity and specificity that are useful for the early diagnosis andprediction of cancers as well as the monitoring of drug effects. In collaboration with the teams of the 'International CancerBiomarker Consortium' led by Dr. Lee Hartwell of the Fred Hutchinson Cancer Research Center, we are focusing on identifyingbiomarkers for the early detection of liver, stomach, colon and other cancers that will help to treat cancer patients moreeffectively and efficiently, and ultimately to raise the survival rate of cancer patients.

ResearchersHyang-Sook Yoo [email protected] Cancer genomics- Cell cycle and signaling, yeast genetics and gene expression

Jeong-Heon Ko [email protected] Discovery of biomarkers that show a change both in quantity and

quality with a high positive prediction value.- Functional studies that relate the candidate biomarker proteins to the

biology of cancer

Eun Wie Cho [email protected] Discovery of autoantibody-based biomarkers that show a change in

the serum of cancer patients

Sang Jick Kim [email protected] Development of new antibodies for recognizing specific glyco-branch

Yong-Sam Kim [email protected] Discovery of biomarkers that show a change in terms of both quantity

(amount of protein in serum level) and quality (aberrant glycosylation)with a high positive prediction value

- Biological validation of cancer biomarker candidates

Research Areas- Discovery of biomarkers that show a change both in quantity and

quality with a high positive prediction value in gastric, liver, colon andlung cancer

- Functional studies that relate candidate biomarkers to the biology ofcancer

- Role of glycolipid in the apoptotic death of mammalian cells.- Discovery of auto-antibodies produced during cancer progressions as

cancer biomarkers- Development of new glyco-recognizing antibodies using phage display

AchievementsIdentification of candidate biomarkers for colon cancerAbout 100 candidate proteins that are assumed to be involved in thedevelopment and malignancy of cancer were identified in the coloncancer model cell line WiDr and patients’ serum. The identificationmethod and proteins were patented.

Effect of an aberrant glycosylation of TIMP-1 on cancermetastasisAberrant glycosylation of TIMP-1 is responsible for the mitigatedinhibition of both matrix metalloproteinase (MMP)-2 and MMP-9,and this aberrancy elevates the invasive/metastatic potential of coloncancer cells.

Effect of an aberrant glycosylation of PTPk on cancercell migrationPTPk undergoes an aberrant glycosylation in GnT-V-overexpressingWiDr cells, and the aberrantly glycosylated PTPk was vulnerable toproteolytic cleavage. The enhanced cleavage of PTPk in GnT-V-overexpressing cells was responsible for the mitigation of thehomophilic binding capacity, resulting in an increase in cancer cellmigration.

Role of GM3 in glutamate-induced cell death GM3 was up-regulated in the mouse hippocampal cell line HT22 deathcaused by glutamate. Twelve-lipoxygenase (12-LOX) was recruited toganglioside-enriched microdomains in a GM3-dependent mannerduring oxidative glutamate toxicity.

Selected PublicationsYong Sam Kim, ..., and Jeong-Heon Ko(Corresponding)“Functional proteomic study reveals that N -acetylglucosaminyltransferaseV reinforces the invasive/metastatic potential of colon cancer throughaberrant glycosylation on TIMP-1” Mol. & Cell. Proteomics (in press)Yong Sam Kim, ..., and Jeong-Heon Ko(Corresponding)Identification of target proteins of N-acetylglucosaminyltransferase Vin human colon cancer and implications of protein tyrosine phosphatekappa in enhanced cancer cell migration Proteomics 6: 1187-1191(2006)Yong Sam Kim, ..., and Jeong-Heon Ko(Corresponding)Ganglioside GM3 is involved in neuronal cell death FASEB J. 20: 1248-1250 (2006)

Director Dr. Hyang Sook YooContact Tel +82-42-860-4710


Daejeon-KRIBB-FHCRCResearch Cooperation Center

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Korea BiosafetyClearing HouseKorea Biosafety Clearing House(KBCH) specializes in education and public relations, survey and research, and internationalcooperation as well as abiding by the legal requirements concerning the LMO information that is needed for theimplementation of the "Cartagena Protocol on Biosafety" and the "Act on Trans-boundary Movements, etc. of LMOs"

ResearchersHo-Min Jang [email protected] Biosafety information management, especially in accordance with the

Biosafety Protocol and the "Act on Trans-boundary Movements, etc. ofLMOs"

Bong-Suk Sung [email protected] Research and analysis of the trade of LMOs

Gi-Cheol Kim [email protected] Research and analysis on laws, policy, socio-economical issues, etc.

about LMOs and Bioindustry

Gook-Che Jeon [email protected] Biosafety information management

Jeong-Suk Jo [email protected] Research and analysis on R&D about LMOs and Bioindustry

Mi-Hee Jeon [email protected] Communication and public relations

Research AreasLegal requirements concerning LMO informationA key priority in the effort to establish national informationmanagement and sharing systems for LMOs is to achievesynchronization and interoperability between the information systemsof all organizations involved in biosafety informational activities

Communication and public relationsPromotion of public awareness and participation through online andoffline communication

Survey and researchResearch and analysis on law, regulations, R&D, and socio-economical issues, etc. about LMOs and biosafety

International cooperationCollaboration with foreign organizations and participation ininternational meetings about LMOs and biosafety

AchievementsInformation exchange through biosafety informationsystemsKorea ratified the Biosafety Protocol in October 2007 and willimplement the "Act on Trans-boundary Movements, etc., of LMOs" onJan. 1, 2008. The KBCH has already launched its national role ofregistering Korean LMO information at the BCH. A key priority in theeffort to establish national information management and sharingsystems for LMOs is to achieve synchronization and interoperabilitybetween the information systems of all organizations involved inbiosafety informational activities. The KBCH presented itself at the Biosafety Protocol relatedmeetings(Conference of the Parties serving as the meeting for theParties to the Cartagena Protocol on Biosafety, etc.), BCH operatingexperts' meetings, etc. It also supported six Asian countries - Thailand,Indonesia, Mongolia, Myanmar, the Philippines and Bhutan for theBCH operating experience exchange. In particular, the KBCH providedsupport for the improvement of biosafety capacity by dispatchingbiosafety experts to Pakistan in 2007.

Promotion of communication among the public, specialistsand stake-holdersThe Biosafety White Paper and the Biosafety Journal have beenpublished and distributed to biosafety stake-holders. In 2007, the KBCHdistributed PR booklets and leaflets by levels(beginning, intermediate,advanced), and produced LMO laws and regulations as well as PRmovies. The KBCH also made efforts to promote awareness of LMOsthrough educational seminars for the public including 'Knowingcorrectly about LMOs,' and 'LMO laws and regulations.' Furthermore,it has been running a Biosafety essay competition for undergraduateand graduate students. The competition was expanded to includemiddle and high school students in 2007.

In-depth investigation/analysis on biosafety and LMOsSurveys on the public perception of Biotechnology and LMOs have beencarried out since 2003 to gain a better understanding of changes inconsumer perceptions. Surveys of companies' perception of and attitudetowards biotechnology and LMOs were also carried out in 2007. Theresults have been taken into consideration as an important factor formaking effective programs for education and promotion. The KBCHalso investigated and analyzed "Methods for implementing LMO Lawsefficiently" and "The current state of LMOs for industrial use" and soon. The KBCH surveyed the worldwide LMO authorization status as wellas the Korean status of LMOs and Bio imports and exports, and alsoanalyzed the trends on policy and regulation, R&D, society andeconomy, public perception and risk assessment and review.

Director Dr. Ho Min JangContact Tel +82-42-879-8300


Korea BiosafetyClearing House

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Biotech PolicyResearch CenterNominated and established by the Ministry of Science and Technology in 2004, the Biotechnology Policy Research Center aims toassist the government in establishing policies on biotechnology. To do so, the center investigates domestic and international policyinformation regarding biotechnology and runs a portal site (http://www.bioin.or.kr/) to enhance the public’s understanding ofbiotechnology and biotechnology policy. The center also develops and provides biotechnology statistics, bibliometric, and marketanalysis. Additionally, the center organizes and supports various networks among the expert groups associated with the field ofbiotechnology.

ResearchersByung-Hwan Hyun [email protected] Director of the center

Seong-Hoon Moon [email protected] Policy planning and policy research, analysis of industrial trends

Young-Cheol Kim [email protected] Policy planning and policy research, biotechnology white paper

Dong-Sub Yoon [email protected] Policy planning and policy research, statistical development: Gathering

and arranging statistical data of BT from domestic and overseascountries

Eun-Sung Kim [email protected] Policy planning and policy research, international collaboration

Cheon Moo Lee [email protected] Policy planning and policy research, analysis of institutional trends

related to bioethics and biosafety

Moo Woong Kim [email protected] Analysis of technological trend and patent analysis

Eun Jung Kim [email protected] Analysis of technological trends and patent analysis

Su Gil Kim [email protected] Organizing expert networks and monitoring BT information

Mi Jeong Park [email protected] Management of bioportal (i.e. BioIn) and the monitoring of BT

information

Seong-Hoon Park [email protected] Management of bioportal (i.e. BioIn) and knowledge resource system

(i.e. KR), PPT design

Min-Jung Oh [email protected] Management of budget, public relations

Seung-Hoo Shin [email protected] UST student (University of Science & Technology), management of

technology and policy

Research AreasPolicy planning- To plan a comprehensive national policy or strategies to foster the

research and development of biotechnologyPolicy research- To investigate technology, industry, institution, and policy information as

well as to conduct relevant statistics, patent map, and bibliometricanalysis

Information gathering/Distribution- To run a portal site (http://www.bioin.or.kr/) in order to provide

systematic information regarding biotechnology and biotechnologypolicy as a whole

Public relations - To publish biotechnology white papers and to organize public

workshops in order to enhance the public’s understanding of biotechnologyInternational collaboration- To facilitate effective international collaborations with foreign policy

institutes and establish a foreign base for the center

AchievementsMonitoring BT trends and offering informationThe center monitors technology, industry, policy and institutions withregard to BT, and provides the gathered information to policymakers andresearchers to help with their planning of new policies and projects.- Technical Trend Reports (2007): Glycomics and Carbohydrate

Medicine; Human Genomics and Relevant Techniques; Brain Researchand Relevant Techniques; Biological Resources; Bioremediation; NewDrug Development

- Industrial Trend Reports (2007): A Study on the Validity Assessmentof the Establishment of BT Infra and Facilities in Kyung-Gi Biocenter;An Analysis of the Trend of Bioindustries in Major Countries; 2007Biotechnology Statistics; A Study on the Domestic and International Marketand Prospects for New Bio-drugs

- Institutional and Policy Trend Reports (2007): Technology Assessmentand Public Understanding of Biotechnology; Planning of the Promotionof R&D Related to Aging Society

Patent analysis and statistical development- Patent map and papers analysis system has been devised to assist the

government in planning R&D strategy for national projects and to setthe direction for BT research projects.

- The center has gathered every item of domestic and overseas statisticaldata on biotechnology and categorized them by investment, humanresources, industry, and technology up to the end of 2006. Released withstatistical reports, the data has been used for policymaking.

- Patent Map Reports (2007): FDA-approved Drug; Core Technologies relatedto the Bio-Green 21 Project; Platform Technologies related toConstruction of Antibody Therapeutics; Technological Trends inTherapeutics for Atopy

White paper and national guidebook for BT- The center has published comprehensive white papers that cover national

policies on BT and R&D status, and domestic and overseas trends in BT.- The guidebook, which encompasses national policies, domestic research

status, systems and laws on BT, is used for the global promotion ofKorea’s biotechnology industry.

Network of BT expertsExperts in BT have formed a broad community for policymaking andplanning in the field of BT. The network has worked on analyzingcurrent issues and developing future technologies.

Director Dr. Byung Hwan HyunContact Tel +82-42-879-8370


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KOreanBioInformationCenter(KOBIC)Our goal is to develop a bioinformation infrastructure that will facilitate the efficient acquisition, analysis, and circulation of theever-increasing corpus of bioinformation. We are developing a coherent network among the major genome researchorganizations, building integrated databases, and supplying an information analysis system. By providing such a sharedinfrastructure, we intend to raise the standard of Korean bioinformatics research facilities in order to lift Korea into the top fiveworld ranking at the very least in terms of quality and quantity.

ResearchersJong Hwa Bhak [email protected] Interactome- SNP analysis

Sunghoon Lee [email protected] Proteome- Evolution

Bo Kyeng Hou [email protected] Systems biology- Bioinformatics

Byungwook Lee [email protected] Database- Comparative genomics

Daeui Park [email protected] Ageing- Protein-Protein Interaction

Woo-Yeon Kim [email protected] Repeatome- SNP analysis

Sang-Bae Kim [email protected] Microarray

Tae Hyung Kim [email protected] Bioinformatics- Sequence analysis

Chang-Bae Kim [email protected] Comparative genomics- Biodiversity Information

Seungwoo Hwang [email protected] Microarray- SNP

Research AreasDevelopment of an integrated nationalgenomics database- Developing an integrated database of genome

information for humans, animals, plants, andmicroorganisms and a platform for joint use

- Developing support systems for the identification/analysis of useful genes and core platform technologyfor bioinformatics

Development of a tight network and databasetransaction system for major researchorganizations- Building a network among genome research

institutions to serve as a center for sharing genomicinformation

- Cyber community (BioWiki, BioBlog, BioNew, etc.) tofacilitate the sharing of researchers' interests andresources

Distribution of bioinformatics research, analyticaltools, and package development- Distribution of an EST cluster and alternative splicing analysis software- Integrated platform (BioWorkbench) for analyzing biological data

upon automated bioinformatics infrastructure- Framework system(BioPipe) oriented by workflows and WEB 2.0 to

analyze large-scale biological problems

Support of large-scale bioinformatics analysis andcollaborative research- Developing SNP information analysis, analysis systems/software- Offering support for the Brassica genome analysis map

Development of bioinformatics technology and support- Developing/distributing software for information analysis of gene

regulation/function- Analyzing genome function and proteomes

AchievementsInfrastructure for information researchWe operate the fifth largest genome/proteome sequence retrievalsystems (SRS) in the world, which can be used as both a biologicaldatabase and an analytical tool. We acquired public resources andautomated analysis, built an automated analytical core system, anddeveloped bioinfrastructures.

Domestic and international bioinformatics educationalservicesWe held the 6th Korea-Japan-China Bioinformatics Training Course(Mar. 27-30, 2007). We also offer cost-free bioinformatics analysis tovarious research institutions, and give periodic lectures, providingbasic training in bioinformatics.

Development of international networks for bioinformaticsand the establishment of MOUsExchange agreements were made with Chungbuk NationalUniversity(2007.3.15) and the National Association of BiodiversityInstitutes (NABI, 2007.3.6).We have developed the following bioinformatics tools, databases, andservices :- Patome- SNP@Ethnos, SNP@NMD, SNP analysis server(SNP@domain)- BioPipe(biopipe.net), BioWorkbench, BioCommunity(biocommunity.org)- Primate Genome Database(repeatome.org)- Localizome server(localizome.org)- Array Port(microarray data analysis system)- Gene Set Analyzer- BioPedia(biopedia.org, an openfree bioinformation hyper text system)- BioPeople(biopeople.org, an openfree biological researchers' directory)- BioSpecies(biospecies.org, an openfree biodiversity directory)- BioCorea(biocorea.org, an openfree directory for Korean biotechnology)

Selected PublicationsJong Hwa Bhak(Corresponding) Impact of transcriptional propertieson essentiality and evolutionary rate Genetics 175: 199-206 (2007) Byungwook Lee(Corresponding) Patome: a database server forbiological sequence annotation and analysis in issued patents andpublished patent applications Nucleic Acids Research 35: D47-D50 (2007)Areum Han(Corresponding) SNP2NMD: A database of humanSNPs (single nucleotide polymorphisms) causing NMD(nonsense-mediated mRNA decay) Bioinformatics 23(3): 397-399 (2007)

Director Dr. Jong Hwa BhakContact Tel +82-42-879-8500


KOreanBioInformation Center(KOBIC)

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InternationalBiological MaterialResearch CenterWe aim to procure indigenous biological materials from four overseas' regional centers and their neighboring countriesthrough legal routes within the scope of international collaborative research projects. Our mission is to provide researcherswith biodiversity materials and ethnobotanical information including indigenous medicinal knowledge, and to establish thenation's core infrastructure for developing new natural drugs and nutraceutical products, along with other commerciallyimportant natural products.

ResearchersHyeong-Kyu Lee [email protected] product chemistry-Immunology

Joong-Ku Lee [email protected] Plant taxonomy- Biodiversity

Research Areas- Establishment of four collaborative biological material research

centers for the collection and preparation of biological materialsworldwide

- Establishment and operation of a comprehensive system anddatabase in order to manage biological materials and relatedtraditional medicinal knowledge procured from four regional centersand their neighboring countries

- Establishment and operation of a supply system for efficient provisionof biological materials to leading research groups within the scope ofassigned projects

- Development of new natural drugs, nutraceuticals and othercommercially-important natural inventions.

AchievementsEstablishment of an International Biological MaterialResearch Center- The center’s organization has been completed. Items of quipment

and facilities have been set up : highly-sensitive items of equipment(LC/MS and electric microscopes) and an expanded herbarium(storagecapacity of over 100,000 voucher specimens). In addition, nearly 7,000plant extracts have been distributed so far.

- Procurement of Foreign Biological Materials

ChinaEstablishment of the Korea-China Biological Material Research Center- Located in Cumming, Yunnan- Personnel appointed(1 expert from Korea, 2 from China) and research

equipment set up- Biological materials(2,246 extracts) with ethnobotanical information

Central America- Establishment of the Korea-Costa Rica Biological Material Research Center - Building personnel and research equipment to be ready by January 2008 - The Korea International Cooperation Agency provides funds for buildings

and most of the equipment- Biological materials(400 dried plant species and extracts) with

ethnobotanical information

South-east Asia- Working group meetings held for the establishment of a collaborative

center (June 2007)- Biological materials(309 dried plant species) including oceanic

biological materials from Micronesia

South Africa- Working group meetings held for the establishment of a collaborative

center (June 2007)- Biological materials(500 dried plant species) including oceanic

biological materials from Micronesia

Director Dr. Hyouk JoungContact Tel +82-42-860-4300


Information Biological MaterialResearch Center

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The 21st Century Frontier R&D ProgramThe Center for Functional Analysis of Human Genome

The completion of the human genome sequence and the recent improvements made in chip technologies and bio-informaticapproaches have provided enormous opportunities for the prevention and treatment of incurable diseases such as cancer.The R&D program for this center has been initiated i) to create knowledge, biomarkers, and molecular targets by genomicapproaches for the development of diagnostics and therapeutics for stomach and liver cancer disease which are prevalent inKorea, and ii) to compete with advanced countries in the area of functional genomics of human beings. Our aims are i) tocollect on a large scale and analyze functionally and clinically the novel biomarkers and molecular targets more closelyassociated with tumorigenesis by genomic approaches, and thereby ii) to develop diagnostics and candidates for the targetedtherapy of stomach and liver cancers.

Researchers Dong-Soo Im [email protected] Functional analysis and validation of genes associated with tumor - Gene therapy of cancer

Young Il Yeom [email protected] Genomic analysis of cancers and identification and functional

validation of therapeutic targets - Cancer gene therapy

Yong Sung Kim [email protected] Epigenomics in gastric and colon cancers - High-throughput LOH genotyping associated with gastric cancer

Jeong-Heon Ko [email protected] Discovery of bio-markers that show a change both in quantity and

quality with a highly positive prediction value in gastric,hepatocellular, colon, and lung cancers

- Functional studies that relate candidate bio-markers to the biology ofcancer

Jung-Joon Lee [email protected] Functional analysis and validation of a new gene to determine

suitability for a molecular target in cancer therapy

Hee Gu Lee [email protected] Preparation and production of antibodies against peptides,

recombinant or fusion proteins from noble genes- Identifying for cancer bio-markers and developing their uses in

diagnostics

Sang Chul Lee [email protected] Diffentiation of stem cell- Discovery of cancer bio-markers by proteomic approaches

Sang Seok Koh [email protected] Genomic analysis of cancers - Development of human monoclonal antibodies for cancer

therapeutics

Cho-Rok Jung [email protected] Functional analysis and validation of genes associated with tumor - Gene therapy of cancer

Research Areas- Development of cancer diagnostics- Functional analysis of molecular targets for innovative cancer-

therapeutics- Development of candidates (small molecules and antibodies) for

targeted cancer therapy- Establishment of a high-throughput functional screening system of

genes at genomic level

AchievementsHuman gene bankWe established a human gene bank at KRIBB in 2003 that possesses38,000 Korean unigenes and 10,000 full-length human cDNAs, andhave distributed the genes to the interested parties. About 40,000 geneshad been sent out domestically and to foreign countries by 2006. Wewill continue this service to take the lead in functional genomics.

Gene chipWe have selected a set of 250 genes that can serve as survival predictionbiomarkers for liver cancer patients, and prepared a prototype genechip containing the biomarkers that can be useful for patienttreatment and tailored medicine (Patented in Korea, 2006).

Novel diagnostic and prognostic biomarker for gastriccancerMac-2 binding protein (Mac-2BP) is a secreted tumor antigen that iselevated in many cancers including gastric cancers and implicated intumor metastasis. Mac-2BP protein is highly expressed in most gastriccancer cell lines and tumor tissues. Evaluation of intracellular andsecreted Mac-2BP levels via ELISA indicates that Mac-2BP is expressedand secreted more abundantly in gastric cancer patients than othertumor patients and healthy donors (Patented, 2006). The elevatedserum Mac-2BP level in gastric tumor patients is also significantlyassociated with distant metastasis and the more advanced stage of atumor. In 2007, we licensed out the Mac-2BP biomarker to HanribLife-Tech Inc. in Korea, which will develop a kit for the diagnosis andprognosis of gastric cancer.

Novel target We found that E2-EPF UCP targets the tumor suppressor VHL fordegradation and is associated with tumor growth and metastasis. TheUCP may be a new molecular target for the therapeutic intervention ofhuman cancers (Nat. Med. 12, 809-816, 2006).

International collaboration with PfizerWe have initiated cooperative research with Pfizer, a globalpharmaceutical company, for pursing innovative biomedical research.Pfizer has sponsored a research project, in which genes associated withliver cancer will be evaluated and determined whether they aresuitable targets for drugs.

Director Dr. Dong Soo Im Contact Tel +82-42-860-4400


The 21st CenturyFrontier R&D Program

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The 21st Century Frontier R&D ProgramPlant DiversityResearch Center

We graft plant resources and modern bioengineering technology to create high value-added products such as natural drugs andnutraceuticals. We also apply gene isolation and manipulation technology to develop new types of transgenic medicinal plants.

ResearchersHyeong-Kyu Lee [email protected] Establishment of a plant extract bank of Korea- Natural Product Chemistry- Immunology

Joong-Ku Lee [email protected] Establishment of a Seed Bank for wild and endangered plant species

in Korea- Plant taxonomy- Biodiversity

Jung-Joon Lee [email protected] Development of nutraceuticals for improving erectile dysfunction- Discovery of bioactive molecules- Molecular mechanism of bioactive molecules

Byoung-Mog Kwon [email protected] Development of nutraceuticals and biologically active compounds for

cancer therapy- Chemical genomics- Natural products

Young-Kook Kim [email protected] Development of natural drugs for hyperlipidemia- Cholesterol acyltransferase- Cholesterol metabolism

Research AreasInfra projects- Constructing and managing the plant extract bank, plant

bioinformatics system and seed bank for wild and endangered plantsspecies

Development of plant-derived natural drugs andnutraceuticals- Grafting plant resources and modern bioengineering technology to

create natural drug materials for the treatment and prevention of diseases- Developing high value-added nutraceuticals

Development of high value-added transgenic plants- Analyzing useful genes in plants, and using gene isolation and

manipulation technology to create new types of value-addedtransgenic medicinal plants

Collection, preservation and cultivation of plants species- Collecting and classifying plant resources originating from the Korean

peninsula- Nurturing superior wild flower and tree cultivars

AchievementsTechnology transfer with corporationsIn the area of natural drugs and nutraceuticals research, we registered35 patents and transferred three different technologies, resulting in334 million won in technical fees, thus contributing to the activity ofnatural drugs development and its related industries. We also securedand accumulated important infra-technologies.

Preclinical/clinical trials of natural drugs andnutraceuticals15 candidates for natural medicines and nutraceuticals were advancedand four are currently in clinical trials, and two are currently inpreclinical testing. These are the results of extensive knowledge andpreclinical experience of plant resources and oriental medicine. Webelieve that this serves as an example, showing the possibility ofdeveloping high value-added natural medicines/nutraceuticals valuedat billions of won in a short period.

Continuation and reinforcement of infra projectA plant extract bank (Korean Plant Component Bank) with about5,200 domestic/foreign samples was constructed, and it distributed25,328 extracts to domestic academic/industrial/research institutions.We set up a center for the research of plant genome function andconstructed 280,000 EST of genetic information and microarraydatasets. For the seed bank for wild and endangered plant species, wecollected 493 classes of seedlings of rare and endangered plants,examined their rate of germination (460 classes), studied theirfeatures (30 classes) and constructed chromosome databases (83classes).

Director Dr. Hyouk JoungContact Tel +82-42-860-4300



8584

The 21st Century Frontier R&D ProgramMicrobial Genomics& Applications Center

We aim to promote the discovery of novel genes, valuable biomolecules, engineered microbes and innovative bioprocesses.The Microbial Genomics and Applications Center focuses on developing technology platforms to utilize information about thegenome function obtained from analyses of microbial genomes isolated from diverse environments.-Elevate Korea to one of the top countries in the global microbial industry-Create a market exceeding one billion dollars-Construct a global knowledge infrastructure by acquiring key intellectual property rights

Researchers Jung Hoon Yoon [email protected] - Biodiversity - Metagenome

JiHyun F. Kim [email protected] - Microbial Genomics- Bacteria-host interaction

Myung Hee Kim [email protected] Protein structures and functions- Macromolecular crystallography

Kwang-Hee Son [email protected] - Microbial metabolome- Small molecule drugs from natural products

Research AreasMicrobial diversity and metagenomes- Isolation and characterization of marine microbes, extremophiles,

and symbiotic microbes - Construction of metagenomic libraries- Screening microbes with high industrial potential

Functional genomics- Functional genomics and proteomics- Comparative genomics- Analysis of metabolic pathways and networks- Bioinformatics and a portal for microbial genome information

Metabolic engineering and genomic applications- Identification of lead molecules from microbial resources- Metabolic engineering and genome engineering- Genome information-based enzyme evolution- Novel molecular bioprocesses- Novel bioconversion technologies

AchievementsAchievement of number one in the world’s new bacteriadiscoveryWe have secured more than 200 species of microbial resources andregistered over 100. Over 3,000 Mbps of effective metagenomes wassecured.

Analysis of microbial genomeGenome functional research was carried out for six microbial groupsfrom Tricholoma matsutake, kimchi lactic acid bacteria, entericbacteria, actinomycetes, yeasts, and V. vulnificus. We also securedOmics information in order to construct a data analysis system for theOmics of E. coli B, and were successful in completing a genomesequence database and a comparative analysis system.

Reprogrammed microbial cells and applicabletechnologyWe developed an astaxanthin hyper-production strain through afunctional genomic and pathway engineering approach(KRIBB), andanalyzed the metabolic pathway of sulfur-containing amino acidusing Corynebacterium genome information; we developed an L-methionine hyper-producing strain by genome based cell-reconstruction(CJ Co., Ltd); we developed medicinal glycosides by abioconversion-based process using glycosyltransferases andcharacterization (Chonnam Univ.); we developed a production systemfor virus-like particles (VLP) by genome-wide control of yeast (MogamBiotech Research Institute); we engineered a glucoamylase promoterthat has been widely used for the recombinant protein in Aspergillusniger; and we succeeded in developing a biotransformation process forvalienamine, a major intermediate for the chemical production ofvoglibose (Yuhan Co.)

Director Dr. Tae Kwang OhContact Tel +82-42-879-8200



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International CooperationKRIBB has made every effort to become an internationally renowned research institute in the field of biotechnology through intensive cooperation to beachieved by building strategic global networks and carrying out joint research projects, in particular with major national and global pharmaceuticalcompanies such as Pfizer Inc.

Global Research ActivitiesKRIBB was involved in 16 international research activities in 2007, working with leading scientific organizations across the globe. KRIBB works activelywith our key partners to develop and support relationships best suited to achieving mutual goals.

Global Cooperation NetworksKRIBB has signed 15 Memorandums of understanding and agreement in 2007 and has strengthened its ties with 99 institutions in 28 countries (120agreements) in total to prepare for the bio era and to build a world-class research environment.

Other Major Achievements

Visit of foreign delegates312 foreign delegates from overseas visited KRIBB in 2007 to establish international networks and discuss future research cooperation. Important visitorsincluded Catherine BRECHIGNAC, President of CNRS, Dr. Bharat Chowrira, Vice President of Merck & Co.,Inc., and Dr. BJ Bormann, Vice President ofPfizer, etc.

International seminars and symposiaKRIBB hosted or organized a number of seminars, symposium and meetings.

Appointment of foreign adjunct research fellowsKRIBB appointed 8 foreign adjunct research fellows, including Dr. Anthony Watts, Professor of Oxford University, Datin Dr. Ann Anton, Professor ofUniversiti Malaysia Sabah, and Dr. Hiroyuki Osada of RIKEN, asking them the favor of advice and guidance to promote research cooperation betweenKRIBB and other overseas institutions.

Project Manager

Jeong-Heon Ko

Min-Gon Kim

Jong-Seog Ahn

Jong Hwa Bhak

Dae-Yeul Yu

Hyouk Joung

Inpyo Choi

Sang-Soo Kwak

Project Title

International cooperation for cancer biomarkerdevelopment

Development of a cancer-diagnosis biosensortargeting post-modified proteins

Bioactive Metabolite Research Center

Development of a Korea-China-Japan bioinformatics network

Generation of ENU mutant mice and studies onthe in vivo function of the mice

Establishment of a foreign biological resourcescenter and central hub in Korea

Development of platform technology for cancerimmunotherapy

Understanding of the molecular mechanism ofdrought tolerance in plant and development ofindustries

Country

USA

USA

Japan

Japan,China

Japan,China

Peru

USA

China

Cooperating institute

FHCRC

FHCRC

RIKEN

DNA Data Bank of Japan, National Institute of GeneticsShanghai Center for Bioinformation Technology

RIKENNanjing University

Amazonian Biodiversity Research

University of Washington School of MedicineFred Hutchinson Cancer Research Center

Shanghai Institutes for Biological Sciences

Country

International CooperationNetwork

Canada

Indonesia

Laos

Malaysia

InstituteAsia-Pacific Molecular Biology Network (A-IMBN)

The Consortium for the Barcode of Life(CBOL)

Biodiversity Institute of Ontario (BIO)

The Agency for the Assessment and Application of Technology (BPPT)

IDB

Souphanouvong University

KEDAH BioResources Corporation

Country

Nigeria

South Africa

Spain

Taiwan

USA

Institute

IAMRT

CSIR

University of Valencia

Industrial Technology Research Institute (ITRI)

yet2.comLARTA-BANSDPfizer Inc.University of Utah

Title

Foreign Scholar Seminar (Sir John Skehel, Royal Society, UK)

KRIBB-Harvard Joint Symposium

KRIBB- BIOTEC Joint Symposium

KRIBB-Indonesia Joint Symposium

KRIBB-ITRI Joint Symposium

6th Osong International Biosymposium

Topic

The Origin of Pandemic Influenza

Cancer/Medical Research

Biosensor and Biochip

Recent Research Status of Biotech in Two Countries

Recent Research Status of Biotech in Two Countries

Emerging Plant Biotechnology Trends and Industrial Implications

Manager Mr. Jin Sun Choi Tel +82-42-860-4730Fax +82-42-860-4739 E-mail [email protected]

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Technology Commercialization Support for TechnologyInformation

Information Collection and Current StatusThe KRIBB Digital Library(http://library.kribb.re.kr) has made it a priority to collect electronic materials such as electronic journals, electronic books, andweb databases for providing information more rapidly and improving users' convenience. The library has been subscribing to electronic journals since1998 and participating in the KESLI consortium. At the moment, the number of electronic journals to which the library subscribes is 5,770 titles, which ismuch higher than that of 62 printed journals. More than 360,000 articles are downloaded in the PDF and HTML formats each year.

Research Results and Information DatabasesThe library has databased KRIBB's research results on the web with 6,532 papers, 1,140 patents, and 1,340 research reports (Total: 9,012) for web users. Byusing the iLIPS program, users can search and gain access to all data including printed books, electronic journals, and electronic books within the library.

Document Delivery Service (DDS)To solve the problems of the increasing amount of information and the lack of collections in the library, the library has made agreements with theKorean Medical Library Association (KMLA), National Digital Science Library (NDSL), Korea Institute of Science and Technology Information (KISTI), andKorea Education and Research Information Service (KERIS) so that researchers can obtain copies of original materials. The library has provided morematerials (1,745 items) rather than received requests for original copies (1,104 items), which means that it has contributed to promoting documentdelivery among domestic libraries. In particular, users can receive materials within two or four days.

Management of Papers and Computerized WorkIn deciding promotion and assessing personal performance, every paper published by researchers has been managed with a special program (MIS)according to the criteria for paper assessment. The program is used for registering and inputting papers, building a full-text database, checking SCI and IF,issuing statistical data, reporting the results in and out of the KRIBB, providing a service for web users, and publishing references(SCI reference materials,vol. 5, in Aug.). Every process has been through the computer for managing papers and books, requesting for copies DDS, and paying copying fees.

- Searching for excellent patent technologies, and promoting technology commercialization of research outcomes through partnershipsbetween industry and research institutes.

- Identifying and nurturing excellent bio-ventures, such as the enterprise invested by institute and the start-up company by researcher

Main Function Patent management team- Intellectual property management / patent information and program

management / promising technology search

Technology commercialization team- Technology transfer / technology marketing / technology commercialization

support / establishment of the enterprise invested by institute

Major Policy ProjectTechnology Licensing Organization Business Support (Korea Technology Transfer Center)- Promoting technology industrialization through the researcher's retention

ability and the outcome of mutual collaboration between industry,universities, and research institutes.

- Supporting resolution for troubled technology to small and medium biobusinesses and venture businesses, and also the activities between industry,university, and research institute.

Technology Business Incubator (Small and Medium Business Administration)- Increasing the possibility of success of the launch of a new business by

constructing an all-period technology business support system, ranging fromtechnology development to production and marketing.

Tenants Information of Bio Venture CenterStart-up company by KRIBB researcher

Enterprise innovation support team- Search and nurture of ventures / incubation and support of new ventures /

industry, academia, institute networking

Technology Innovation Center (Ministry of Commerce, Industry and Energy)- Strengthening technology competitiveness and bio medical-related

technology innovation among small and medium businesseses and venturebusinesses in the Daejeon region.

Regional Innovation System (Ministry of Commerce,Industry and Energy)- Strengthening bio industry's technological capabilities at Daejeon and

constructing a bio industry mecca.

No. Name of Company Main Product No. Name of Company Main Product

Start-up company by non-KRIBB researcher

No. Name of Company Main ProductNo. Name of Company Main Product

Manager Dr. Heung Chae Jung Tel +82-42-860-4540Fax +82-42-860-4549 E-mail [email protected]

Manager Mr. Young Hee Roh Tel +82-42-860-4700Fax +82-42-860-4719 E-mail [email protected]

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3

Anichem Inc.Ace Biotech Inc.

ENZBANK, Inc.

cDNA library construction and DNA Sequencer

Microorganism probiotics

Eugenteck Inc.

ProBionic Corp.

4

5

Functional Bio Material(Bio Capsule)L-Ornithine-L-Aspartate, LipaseThermostable enzyme, new glycoside, functional carbohydrate

1

2

345

6

7

8

NeoPharm Co., Ltd.

Dae Sung MicrobiologicalLabs. Co., Ltd.

Lee's Biotech., Ltd.Bionutregen Co., Ltd.Bio-dreams Co., Ltd.

VaccTech Corp.

Enbioengineering. Co., Ltd.

Enzychem Co., Ltd.

Recycling and treating the foodwaste using edible mushroom spawnContaminated soil restoration service, microorganism used in natural detergentBio Informatics(protein interaction predicting System)Functional food material, cosmetic ingredients businessHerbicide for Landscape ArchitectureDental instruments, fluoric ion introducing instrumentBio diesel, Glycerin

en2t Inc.

H-Plus Eco Ltd.

Oiteck Co., Ltd.

HEALTHKEEPER

Moghu Research Laboratory

Dr. PREVENT Co., Ltd.

EXST Co., Ltd.

9

10

11

12

13

14

15

Ceramide-contained cosmeceuticals (Atopalm, Zeroid)Animal pharmaceuticals (vaccine, AVILAK ABS/probiotics)Liver function improving product (Hep-Clean MH Plus)Anti-obesity functional food (slim&slim diet coffee)Continus pesticide formulation, elicitorHuman vaccine and biomedicine, whitening cosmeceuticalsMicroorganism absorbing filter , water waste treatment, Bioreactor

Antibiotics, tuberculosis drug

Information No. of Material Major Sources

Journals

Books

Printed journalsElectronic journals

Printed booksElectronic books

Research reportsMarket trend reports

Video materialsWeb databases

4695,770

2,10014,240

5,95065020012

Currently 62 journals are subscribed and 16,412 volumes are bound16 publishers including ACS, Blackwell, Elsevier, Nature, Springer, Wiley

Research books or reference materialsebrary, Elsevier, Humana, netLibrary, Springer, Wiley

KRIBB and other institutesDatamonitor, Frost & SullivanKBS MediaDiscoverygate: Beilstein, Delphion, JCR, SciFinder, Scopus

APPENDICES> Outstanding Research Achievements> List of Patents Registered Overseas> List of Technology Transfer> List of Research Projects> Events> Researcher Index> Location

APPENDICES 2007 KRIBB Annual Report

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Date

2007-01-12

2007-03-05

2007-03-13

2007-05-03

2007-05-14

2007-05-16

2007-06-08

2007-08-07

Substance

Mobile liver diagnosissystem

Verification of the functionof host antioxidant enzymeeffective against endotoxin

The top in the world forthe last three years in thepublication of novel bac-terial species

Identification of a newmolecular mechanism inthe development of livercancer by the hepatitis Bvirus

Verification of the mecha-nism of hepatic steatosisby hepatitis B virus

Elucidation of the mecha-nism for hepatitis virusneutralization

Approval for a research-based clinical trial of NKcell therapy

Development of a diagno-stic tool for gastric cancerusing a protein biomarker

Researcher

Bong Hyun Chung

Dae-Yeul Yu

Jung-Hoon Yoon

Young Ik Lee

Dae-Yeul Yu

Seong Eon RyuHyo Jeong Hong

Inpyo Choi

Hee Gu Lee

Note

- A new technology for diagnosing the liver byconnecting a biosensor to the mobile phone forreal-time monitoring of liver patients, and arepresentative model of the ubiquitous healthcaresystem of the future.

- Verification of the role of host antioxidant enzymeresistant against inflammation response to LPS. - Published in Journal of Experimental Medicine(2007. 03)

- Korean scientists published twenty percent ofnovel bacterial species described in 2006.- Published in Int. J. Syst. Evol. Microbiol. (2006. 01)

- We verified that the hepatitis B virus-X proteinderegulates DNA methyltransferase and promotesboth specific regional hypermethylation andglobal hypomethylation involved in the formationof hepatocellular carcinoma.- Published in Gastroenterology (2007. 04)

- Verification that the molecular mechanismhepatitis B virus induces hepatic steatosis- Published in Gastroenterology(2007. 05)

- Explaining the type B hepatitis virusneutralization mechanism through threedimensional structure determination. - Published in PNAS(Proc. Natl. Acad. Sci.USA)(2007. 05)

- Clinical trial for leukemia/tumors using NK cellsdifferentiated from HSC. It will be applied tohaploidentical family patients to preventrecurrence of leukemia/tumor after bonemarrow transfer.- USA patent (pending, 2007)

- Development of a diagnostic and prognostictechnology for stomach cancer using a smallblood sample.- Published in International J. of Cancer (2007. 02)

Date

2007-08-15

2007-08-29

2007-08-30

2007-11-08

2007-11-26

2007-11-28

2007-12-13

2007-12-26

Substance

Construction of systematicdeletion mutants of Schi-zosaccharomyces pombeand its applications to adrug-target screeningsystem

Worldwide first isolationof archaebacteria fromfood

Mining of drug targets byp h o s p h o p r o t e o m i canalysis during neuronalcell death

Development of the DNA chip for the identificationof bird species

Development of a human-ized antibody to TAG-72

Discovery of a highlysensitive cancer biomarkerqualita-tively altered duringcancer progression

Programs and databasesfor tissue-specific alternativesplicing gene, novel GPCRclassification

New anti-influenza com-pound

Researcher

Kwang-Lae Hoe

Jin-Woo Bae

Sang Chul Lee Kwang-Hee Bae

Chang-Bae Kim

Hyo Jeong Hong

Jeong-Heon Ko

Cheol-Goo Hur

Dur-Han Kwon

Note

- A S. pombe deletion project has been completed,and a drug-target screening system has beensetup using the deletion library.

- Worldwide first isolation of archaebacteria fromfood (Korean shrimp jeotgal).- The first case of novel archaeabacterial speciesin Korea.- Published in IJSEM (2007. 09)

- Mining of drug targets toward neurode-generative diseases by phosphoproteme profilingduring neuronal cell death.- Published in Proteomics(2007. 08)

- Development of an oligonucleotide chip basedon the DNA barcode records for the identificationof bird species including migratory birds.

- Humanized antibody to TAG-72 was constructedand its tumor targeting efficacy and biodistributionwere studied.

- GnT-V directs cancer malignancy by inducing anaberration of TIMP-1 glycan, and accordingly theaberrant TIMP-1 can be a prominent cancerbiomarker. - Published in Molecular & Cellular Proteomics(2007. 12)

- The tissue specific genes expressed in humanand mouse tissues are predicted usingbioinformatics and proved by experiment. Thismethod is a fusion technology of BT and IT andenables us to find out the candidate target genesof new drugs.- Published in Computational Biology and

Chemistry

- New anti-influenza compound (Q7R) isolatedfrom natural plant shows broad protectionagainst several type of viruses includinginfluenza viruses.

Outstanding Research AchievementsImages Images

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List of Technology TransfersList of Patents Registered OverseasName of Technology

High expression vector(pcDCM-dhfr)

Microbial consortia for the treatment of industrial wastewatercontaining chlorinated organic compounds

Production of biosurfactant for the treatment of waste-petroleum

Method for cloning and expressing target genes by homologousrecombination

Hybridomas producing mouse monoclonal antibidy HBV pre S1

Development of a diagnostic tool for gastric cancer using protein biomarkers

The high expression vector system in mammalian cells

Humanized antibody to TAG-72

Screening method of agents inducing apoptosis and developmentof RhoY as an anti-cancer drug

Phaffia rhodozyma mutant strains with improved astaxanthin-extractability and the method of astaxanthin extraction therefrom

Novel bifunctional P450 hydroxylase isolated from themetagenome library

Enzymatic production of biodiesel

Production strain & scale-up process development of B-glucosidase using recombinant DNA technology

Application of MIG12 and OIP5 as novel targets in cancer therapeutics

Programs and databases for tissue-specific alternative splicinggene, novel GPCR classification

Technologies for the production of geldanamycin derivatives bybiosynthetical modification

Natural compound having an antiviral effect

Cellular signaling detection technology

Date

2007-01-25

2007-04-02

2007-04-02

2007-04-26

2007-07-16

2007-08-07

2007-10-02

2007-11-15

2007-11-15

2007-11-16

2007-11-23

2007-11-27

2007-11-27

2007-12-03

2007-12-05

2007-12-18

2007-12-18

2007-12-18

Director

Hyo Jeong Hong

Hee-Mock Oh

Byung Dae Yoon

Seung Goo Lee

Hyo Jeong Hong

Hee Gu Lee

Youngwoo Park

Hyo Jeong Hong

Mi Sun WonHwan Mook Kim

Eui Sung Choi

Jong-Seog Ahn

Jung Hoon Sohn

Joon-Ki Jung

Mi Sun Won

Cheol-Goo Hur

Young-Soo Hong

Dur-Han Kwon

Seong Eon Ryu

Company

Celldex Therapeutics,Inc.

Korea Organic FarmingDevelopment Corp.

Korea Organic FarmingDevelopment Corp.

SolGent Co., Ltd.

Santa Cruz Biotechnology Inc.

Hanrib Lifetech Inc.

Hanswha Chemical Corp.

Viromed Co., Ltd.

Seoulpharma Co., Ltd.

Seohae E&T Co., Ltd.

Genotech Co., Ltd.

Daekyung Esco Co., Ltd

EnzBank Co., Ltd

Samchully Pharm. Co., Ltd

WithUSTech. Co., Ltd.SC&T Inc.

Pharmaceutical Co., Ltd.

Kolon Life Science Inc.

Abfrontier

Title of patent

Transformant of yeast producing recombinant human parathyroidhormone and method for producing said hormone

Insecticidal compositions comprising compound having inhibitoryactivity versus CoA: cholesterol acyltransferase

Composition containing cinnamic acid derivatives for preventing ortreating elevated blood lipid level-related diseases



Esterase, its DNA overexpression and production of optically activearyl propionic acids using the same

A peroxidase genomic gene derived from Ipomoea batatas and apromoter thereof

Poly-gamma-glutamate having ultra high molecular weight andmethod for using the same


Method for the diagnosis of cancers by measuring the changes ofGlycosylation of proteins related to tumorigenesis and metastasis andkit for diagnosis of cancers using the same

Pharmaceutical compositions of diaryl-isoxazole derivatives for theprevention and treatment of cancers

Hansenula polymorpha yapsin deficient mutant strain and process forthe preparation of recombinant proteins using the same

Date

2007-02-01

2007-02-09

2007-04-03

2007-04-15

2007-05-18

2007-05-29

2007-07-04

2007-07-04

2007-07-17

2007-07-19

2007-07-31

2007-08-28

Inventors

Hyun Ah Kang et al.

Young Kook Kim et al.

Song Hae Bok et al.

Hyun Ah Kang et al.

Hyun Ah Kang et al.

Bong Hyun Chung et al.

Sang Soo Kwak et al.

Jae Jun Song et al.

Hyun Ah Kang et al.

Jeong Heon Ko et al.

Byoung-Mog Kwon et al.

Hyun Ah Kang et al.

Country

Germany

Russia

Canada

Spain

Japan

U.S.A.

Europe

China

U.S.A.

Australia

U.S.A.

U.S.A.

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List of Research Projects

Projects funded with over 0.1million(USD) are listed

<Fund Source>- KFDA : Korea Food & Drug Administration- KRCF : Korea Research Council of Fundamental Science & Technology- MAF : Ministry of Agriculture and Forestry- MOE : Ministry of Environment

- MOHW : Ministry of Health & Welfare- MOICE : Ministry of Commerce Industry and Energy- MOMAF : Ministry of Maritime Affairs & Fisheries- MOST : Ministry of Science & Technology

Project Title

A project to establish of a biosafety information infrastructure as a focal point of BCH

Development of salt tolerant bioenergy crops by utilizing salt-inducible genesfrom marine cyanobacteria

Purification and characterization of recombinant proteins from transgenic pig milk

The development of an oligo chip for the diagnosis of viruses

Study of the action mechanism of anti-diabetic drugs by transcriptome profiling

Development of short/medium term models for garcinogenicity testing

DNA microarray-based identification of target genes for the drug action ofevodia officinalis and curcuma longa

Development of biomaterials for cosemeceuticals from the bio-resources ofJeju Island

NMR and statistical physics approach to characterize the structural state andfunctional motifs of unstructured proteins

A study on the characterization and quality control of nanomaterials

Detection of developmental abnormalities in cloned gnotobiotic pig fetus duringgestation

Development of bioinformatics tools for isolation of a useful promoter fromSolanaceae genome information

Practical use of induced resistance compounds derived from endophytic andsoil microorganisms

A study on the mode of action for a RhoB-modulating anti-cancer drugcandidate

An integrated information bank for microbial genome research

CO2 fixation by cyanobacteria and production of high-value biomaterials fromtheir biomass

Project Manager

Ho-Min Jang

Jang Ryol Liu

Bong Hyun Chung

Jae Sun Moon

Young Il Yeom

Hyoung-Chin Kim

Young Il Yeom

Jong-Pyung Kim

Kyou-Hoon Han

Bong Hyun Chung

Deog-Bon Koo

Jung Mee Park

Choong-Min Ryu

Mi Sun Won

Jihyun F. Kim

Hee-Mock Oh

Fund

MOICE

MOMAF

MAF

MAF

KFDA

KFDA

KFDA

MOICE

MOST

KFDA

MAF

MAF

MAF

MOST

MOST

MOST

Period

07.01 - 07.12

07.01 - 07.12

07.01 - 07.12

07.01 - 07.12

07.02 - 07.11

07.03 - 07.11

07.03 - 07.11

07.03 - 08.02

07.03 - 08.02

07.04 - 07.11

07.04 - 07.12

07.04 - 07.12

07.04 - 07.12

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

Project Title

Complex natural plants therapeutics for the treatment of hepatitis

Construction and utilization of plant EST DB

Construction of polyketide biosynthesis diversity and development ofpharmaceutical microbila metabolit

Construction of polyketide biosynthesis diversity and development ofpharmaceutical microbila metabolit

Construction of a seed bank of wild and endangered species in Korea

Development of a diagnostic tool for gastric cancer using protein biomarker

Development of environmental risk assessment technologies of living modifiedorganisms

Development of an HTS drug-target screening system using haploinsufficiency

Development of active compounds for lipid lowering

Development of analytical methods for LMOs

Development of anticancer drug candidates by rational biosynthesis ofgeldanamycin

Development of astaxanthin hyperproduction strain by a functional genomic andpathway engineering approach

Development of biological products for monitoring bio-medical functions usinghighly sensitive biosystems

Development of cyanocrops by utilizing photosynthetic syanobacterial genes

Development of a deposit and application systems for biological data

Development of detection system for HCC markers

Development of fully human monoclonal antibodies against a novel target forcancer therapeutics

Development of functional genome resources for gastric and liver cancers andconstruction of their database combined with function information

Development of a Korean hapMap database

Development of LMO risk assessment systems on human health

Development of molecular biological tools for the strain improvement of succinicacid producing Mannheimia succiniciproducens

Development of multi-conditional sylanase from insect microbial genomes

Project Manager

Young Ik Lee

Cheol Goo Hur

Jong-Seog Ahn

Jae Gu Pan

Joong-Ku Lee

Hee Gu Lee

Sung-Uk Kim

Kwang-Lae Hoe

Young Kook Kim

Hwan Mook Kim

Young-Soo Hong

Eui Sung Choi

Hee Gu Lee

Jang Ryol Liu

Bo Kyeng Hou

Eun Young Song

Sang Seok Koh

Nam-Soon Kim

Young Joo Kim

Hyoung-Chin Kim

Ohsuk Kwon

Ho-Yong Park

Fund

MOST

MOST

MOST

MOST

MOST

MOST

MOST

MOST

MOST

MOST

MOST

MOST

MOST

MOST

MOST

MOST

MOST

MOST

MOST

MOST

MOST

MOST

Period

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

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List of Research ProjectsProject Title

Identification and validation of biomarkers for the HCC

Identification of molecular targets for the targeted therapy of hepatocellularcarcinoma and their application to therapeutics development

Integrated functional genomics on the regulatory network of proteinsecretion and modification in yeast: functional and genomic analysis of theprotein quality-control network

Integrated system of national biological resources and genome information

KRIBB technology transfer office project

Live imaging technology for interaction network in living cells

Management of the application of bio R&D products

Management fund for the Center for Functional Analysis of Human Genome

Microbial resources bank

Molecular function of DRG9 in cancers and its validation as a moleculartarget for drug development

National Cosmeceutical Research Center

Project for the management of the PDRC program

Project for the management of the MGAC program

Spatio-temporal bio-imaging of the calcium signaling system

Study of the co-regulation of a disease specific metabolome with proteomeand genome

System development of biological markers for the diagnosis and cure ofcerebrovascular diseases

Target discovery of hepatocarcinoma using hepatitis virus transgenic mouseand clinical validation

Target identification by genome and proteome analysis

Target validation and therapeutic antibody development using the cancergenomic databases

The application of a novel gene associated with tumor growth andmetastasis for the treatment of liver cancer

The international tomato chromasome 2 sequencing project and functionalanalysis for solanaceae genomes

Project Manager

Jeong Heon Ko

Young Il Yeom

Hyun Ah Kang

Jong Hwa Bhak

Joon-Ki Jung

Seung-Goo Lee

Jung-Sook Lee

Dong Soo Im

Jung-Hoon Yoon

Jung Joon Lee

Ick-Dong Yoo

Hyouk Joung

Tae Kwang Oh

Ki Sun Kwon

Sung Goo Park

Young Joo Kim

Sang Chul Lee

Dong Cho Han

Youngwoo Park

Cho-Rok Jung

Cheol Goo Hur

Fund

MOST

MOST

MOST

MOST

MOICE

MOST

MOST

MOST

MOST

MOST

MOHW

MOST

MOST

MOST

MOST

MOST

MOST

MOST

MOHW

MOST

MOST

Period

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

Project Title

Development of new antibacterial compounds against novel targets derivedfrom microbial genomics

Development of nutraceuticals and biologically active compounds for cancertherapy

Development of nutraceuticals to improve erectile dysfunction, to protectliver function, and to potentiate tumor immunotherapy from indigenousacanthopanx sp.

Development of platform technology for natural killer cells differentiationfrom hematopoietic stem cells

Development of a protein production system and protein design for bio-electrical devices

Development of a somatic cell clone monkey

Development of technologies for mass production and in vitro plantregeneration of useful aquatic plant resources by tissue culture

Development of a transgenic plant capable of producing an edible vaccineagainst Alzheimer's disease

Diagnostic target identification for gastric cancer using nucleic acidbiomarkers

Discovery and development of functional microorganisms

Disease specific biomarker discovery by using serum

DNA barcode system for the conservation and management of majororganisms from Korea

DNA chip-based analysis of the dynamics of the genetic network controllingthe calcium metabolism in mammalian systems

Enhancement of plant comoplex resistance and growth rate by manipulatingmembrane signal transduction

Establishment of a centralized bank for animal cells including frozen embryos

Establishment of a plant extract bank

Evaluation of anticancer activity, pharmacokinetics, and preliminary toxicityof anticancer drug candidates targeting RhoB

Exploitation of extremophiles and metagenomes

Functional analysis of the pathogen-associated molecular patterns andeffectors of Burkholderia glumae causing bacterial grain rot in rice, and thedevelopment of disease control methods by the surveillance mechanism ofrice against pathogen

Project Manager

Won-Gon Kim

Byoung-Mog Kwon

Jung Joon Lee

Inpyo Choi

Moonil Kim

Kyu-Tae Chang

Suk Weon Kim

Hyun Soon Kim

Yong Sung Kim

Jung Hoon Yoon

Seong Eon Ryu

Chang-Bae Kim

Young Il Yeom

Stephen Beungtae Ryu

Ki-Hoan Nam

Hyeong Kyu Lee

Song-Kyu Park

Chang Jin Kim

Jae Sun Moon

Fund

MOST

MOST

MOST

MOST

MOST

MOST

MOST

MOST

MOST

MOST

MOST

MOE

MOST

MOST

MOST

MOST

MOST

MOST

MOST

Period

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

07.04 - 08.03

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List of Research ProjectsProject Title

Establishment of lung cancer model mice vulnerable to oxidative stressand elucidation of the related mechanisms

Mechanistic studies and therapeutic applications of a new apoptosis-inducing biofactor

System development for single-cell assay and molecular evolution offunctional biocatalysts

Development of cancer targeting biomolecules and their immobilizationmethods on nanoparticles

Development of a point-of-care testing platform using FRET-basednanosensors

Development of a portable instrument to assess safety of water andagricultural products based on single cellular bio-sensors

Platform engineering center for industrial utilization of protein resources

Designer’s synthetic biotechnology cluster

Development of asthma-related biomarkers based on functionalgenomics and proteomics

Proteome analysis of metabolism related proteins for new secondarymetabolite synthesis

Development of nano-probes for gastric cancer cell surface markers andtheir application to a cell microchip

Genome-wide epigenomic profiling of human embryonic stem cells

System development for application of genomic sequence information

Development of a bio-system for the optimization of label free molecularimaging technology

Development of a non-human primate embryonic stem cell usinginterspecies nuclear transfer

Identification of a genetic marker associated with Korean medicine bygenome-wide SNP genotyping

Study of the redox-mediated cell signaling network

Project Manager

Dae-Yeul Yu

Sangseok Koh

Seung Goo Lee

Bong Hyun Chung

Seung Goo Lee

Jang Ryol Liu

Jae Gu Pan

Jae Gu Pan

Hyeong Kyu Lee

Byoung Chul Park

Min-Gon Kim

Yong Sung Kim

Hong-Seog Park

Sang Jeon Chung

Deog-Bon Koo

Yong Sung Kim

Ki Sun Kwon

Fund

MOST

MOST

MOST

KRCF

MOST

MOST

MOICE

MOST

KRCF

MOICE

MOST

MOST

MOST

MOST

MOST

MOST

MOST

Period

07.07 - 08.07

07.07 - 08.07

07.07 - 08.07

07.08 - 08.06

07.08 - 08.07

07.08 - 08.07

07.08 - 08.07

07.08 - 08.08

07.09 - 08.06

07.09 - 08.08

07.10 - 08.09

07.10 - 08.09

07.10 - 08.09

07.11 - 08.07

07.11 - 08.07

07.11 - 08.07

07.11 - 08.07

Project Title

Therapeutic Antibody Center

Analysis of microbial genome function of a plant-probiotic bacillus and itsapplication

Management of plant diseases by induced systemic resistance

Development of nutraceuticals improving dyslipidemia and respiratoryinflammation using old platycodi radix

Enzyme platform for the production of cellulosic bioethanol

Human and environmental risk assessment of transgenic crops

Development of insecticide and antimicrobial agent using microbes

Genomics and proteomics approaches to drug candidates for the mode of action

Biocatalyst technology innovation: development of in silico based integrativedirected evolution technology

Development and application of glycan chips for diagnostics and high-throughputscreening

Development of next-generation glycan control systems for glycan remodeling

Glycomic approach for the search of cancer biomarkers

Interfacing of nanostructure/biomolecule and its applications

Regional innovation system for Deajeon biotechnology

Support program for policy & information of biotechnology

Anti-metastasis therapeutic antibody development

Construction of fully human monoclonal antibody for the treatment of lungcancer

Developing protein biosensor by engineering of hormone nuclear receptors

Development of protein chip-based bioassay system and biological contents forprotein chip applicaion

Development of targets and preclinical drugs for the tailored therapy of gastriccancer

Development of transgenic cloned pigs for xenotransplantation

Discovery and functional analysis of new anti-cancer target genes using DNAmethyltransferase inhibitors

Project Manager

Hyo Jeong Hong

Seung Hwan Park

Choong-Min Ryu

Hyun Sun Lee

Jung-Hoon Sohn

Stephen Beungtae Ryu

Chang-Jin Kim

Byoung-Mog Kwon

Jae Gu Pan

Hyun Ah Kang

Hyun Ah Kang

Jeong Heon Ko

Bong Hyun Chung

Joon-Ki Jung

Byung Hwan Hyun

Hyo Jeong Hong

Youngwoo Park

Eui-Jeon Woo

Bong Hyun Chung

Nam-Soon Kim

Kyung-Kwang Lee

Bo-Yeon Kim

Fund

MOHW

MOST

MAF

MAF

MAF

MOST

MAF

MOST

KRCF

KRCF

MOICE

KRCF

MOICE

MOICE

MOST

MOST

MOST

MOST

MOST

MOST

MOST

MOST

Period

07.04 - 08.03

07.04 - 08.04

07.04 - 08.04

07.05 - 07.12

07.05 - 08.05

07.06 - 08.03

07.06 - 08.06

07.07 - 08.03

07.07 - 08.06

07.07 - 08.06

07.07 - 08.06

07.07 - 08.06

07.07 - 08.06

07.07 - 08.06

07.07 - 08.06

07.07 - 08.07

07.07 - 08.07

07.07 - 08.07

07.07 - 08.07

07.07 - 08.07

07.07 - 08.07

07.07 - 08.07

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Researcher IndexResearcher

AHN Chi-Yong

AHN Jong-Seog

AHN Jung-Oh

AHN Kyung-Seop

BAE Jin-Woo

BAE Kwang-Hee

BAE Kyoung-Sook

BHAK Jong Hwa

BOK Song Hae

CHANG Kyu-Tae

CHANG Young-Hyo

CHI Seung-Wook

CHO Eun Wie

CHO Hye Sun

CHO Yee Sook

CHOE In Seong

CHOI Eui Sung

CHOI Inpyo

CHOI Sang-Haeng

CHOI Soo Keun

CHU In-Sun

CHUN Hyo Kon

CHUNG Bong Hyun

CHUNG Im Sik

CHUNG Jin Woong

CHUNG Kyung-Sook

CHUNG Sang Jeon

Page

56

48, 97, 99

58

46

70, 95

40, 95

70

78, 101

96

62, 100

70

44

72

52

36

20

54, 97, 99

20, 94, 100

24

54

32

64

22, 94, 96, 98, 102, 103

22

20

32

22, 103

Researcher

HA Tae Hwan

HAN Dong Cho

HAN Kyou-Hoon

HOE Kwang-Lae

HONG Hyo Jeong

HONG Young-Soo

HOU Bo Kyeng

HUR Cheol Goo

HWANG Seungwoo

HYUN Byung-Hwa

HYUN Byung-Hwan

IM Dong-Soo

JANG Ho-Min

JEON Gook-Che

JEON Jae Heung

JEON Mi-Hee

JEONG Dae Gwin

JEONG Haeyoung

JEONG Soon-Chun

JEONG Tae-Sook

JEONG Won Joong

JO Jeong-Heon

JO Jeong-Suk

JOUNG Hyouk

JUNG Cho-Rok

JUNG Heung Chae

JUNG Joon-Ki

Page

22

44, 101

44, 98

32, 95, 99

26, 94, 95, 97, 102

44, 97, 99

78, 99

52, 95, 97, 99, 101

78

34

76, 102

82, 101

74, 98

74

52

74

18

24

60

28

52

82

74

85, 101

32, 82, 101

54, 90

58, 97, 101, 102

Researcher

JUNG Yongwon

KANG Hyun Ah

KANG Jong Soon

KANG Sunghyun

KANG Yong-Kook

KIM Bo-Yeon

KIM Chang Jin

KIM Chang-Bae

KIM Chang-Gi

KIM Chul Ho

KIM Dae-Won

KIM Dong-Uk

KIM Ekyune

KIM Eun Jung

KIM Eun-Sung

KIM Gi-Cheol

KIM Hee-Sik

KIM Hwan Mook

KIM Hyoung-Chin

KIM Hyun Soon

KIM Hyung-Cheol

KIM Jaewha

KIM Janghwan

KIM Jihyun F.

KIM Jong-Pyung

KIM Joong Su

KIM Min Soo

Page

22

38, 96, 101, 102

60

40

36

48, 102

48, 100, 102

70, 78, 95, 100

60

64

24

32

62

76

76

74

56

60, 97, 99

60, 98, 99

52, 100

24

20

36

54, 86, 98

48, 98

64

66

Researcher

KIM Min-Gon

KIM Moo Woong

KIM Moonil

KIM Myung Hee

KIM Nam-Soon

KIM Sang Jick

KIM Sang-Bae

KIM Sang-Hyun

KIM Semi

KIM Seon-Young

KIM Seung Jun

KIM Song-Gun

KIM Soo Jung

KIM Su Gil

KIM Suk Weon

KIM Sung Uk

KIM Tae Hyung

KIM Won-Gon

KIM Woo-Yeon

KIM Yong Sung

KIM Yong-Sam

KIM Young Joo

KIM Young-Cheol

KIM Young-Kook

KO Jeong-Heon

KOH Sangseok

KOH Sukhoon

Page

22, 103

76

22, 100

54, 86

32, 99, 102

72

78

62

26

32

40

70

32

76

70, 100

28

78

48, 100

78

32, 82, 100, 103

72

32, 99, 101

76

46, 84, 96, 99

72, 95, 96, 101, 102

26, 82, 99, 103

64

Researcher IndexResearcher

KOO Deog-Bon

KWAK Sang-Soo

KWON Byoung-Mog

KWON Dur-Han

KWON Ki Sun

KWON Ohsuk

KWON Suk Yoon

LEE Byungwook

LEE Chang-Soo

LEE Cheon Moo

LEE Chul-Ho

LEE Dae Sil

LEE Do Hee

LEE Eun-Gyo

LEE Haeng-Soon

LEE Hee Gu

LEE Ho-Jae

LEE Hong-Weon

LEE Hyeong-Kyu

LEE Hyun Jun

LEE Hyun Sun

LEE Jae-Ran

LEE Joongku

LEE Joong-Ku

LEE Jung Joon

LEE Jung-Sook

LEE Kee Nyung

Page

36, 98, 103

56,96

44, 84, 96, 100, 102

46, 95, 97

38, 101, 103

38, 99

52

78

22

76

34

54

40

58

56

20, 82, 94, 97, 99

46

58

46, 80, 84, 100, 103

18

46, 102

40

46

80, 84, 99

44, 82, 84, 100, 101

70, 101

20

Researcher

LEE Kiho

LEE Kyeong

LEE Kyung-Kwang

LEE Myung Kyu

LEE Sang Chul

LEE Sangku

LEE Seung Goo

LEE Woo Song

LEE Young Ik

LEESunghoon

LIM Yong Taik

LIU Jang Ryol

MIN Jeong-ki

MIN Sung Ran

MOON Jae Sun

MOON Jeong Hee

MOON Seong-Hoon

NAM Ki-Hoan

OH Doo-Byoung

OH Hee-Mock

OH Hyun Woo

OH Min-Jung

OH Sei-Ryang

OH Tae Kwang

PAN Jae Gu

PARK Byoung Chul

PARK Daeui

Page

60

44

36, 102

38

40, 82, 95, 101

46

54, 97, 101, 103

64

28, 94, 99

78

22

52, 98, 99, 103

26

52

52, 98, 100

18

76

60, 100

38

56, 70, 97, 98

70

76

46

87, 101

54, 99, 102, 103

40, 103

78

Researcher

PARK Doo Sang

PARK Hong-Seog

PARK Ho-Yong

PARK Jeong Mee

PARK Jung Sun

PARK Kyung Chan

PARK Mi Jeong

PARK Seong Sup

PARK Seong-Hoon

PARK Seung Hwan

PARK Song-Kyu

PARK Sung Goo

PARK Youngwoo

POO Haryoung

RHO Mun-Chual

RYU Choong-Min

RYU Seong Eon

RYU Stephen Beungtae

SEO Jeong-Woo

SEO Jungmin

SHIN Kee-Sun

SHIN Seung-Hoo

SHIN Yong-Beom

SOHN Jung Hoon

SON Kwang-Hee

SON Mi-Young

SONG Eun Young

Page

70

24, 103

70, 99

52, 98

36

32

76

38

76

54, 102

60, 100

40, 101

26, 97, 101, 102

22

46

54, 98, 102

18, 94, 97, 100

60, 100, 102

64

24

70

76

22

54, 97, 102

44, 86

36

20, 99

Researcher

SONG Jae Jun

SUNG Bong-Suk

WON Mi Sun

WON Young-Suk

WOO Eui-Jeon

WOO Sun Mi

WOO Taeha

YEOM Young Il

YOO Hyang-Sook

YOO Ick-Dong

YOO Jin-San

YOON Byung Dae

YOON Dong-Sub

YOON Jung Hoon

YOON Suk Ran

YOON Sung Ho

YOON Tae Seong

YOON Won Kee

YU Dae-Yeul

YU Kweon

YUN Bong-Sik

Page

64, 96

74

32, 97, 98

60

40, 102

36

24

32, 82, 98, 100, 101

72

48, 101

26

66, 97

76

54, 86, 94, 100, 101

20

54

18

60

34, 94, 103

36

48

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Events

2007KOREA RESEARCHINSTITUTE ofBIOSCIENCE & BIOTECHNOLOGY

2007-01-23 Signing of an MOU with the Consortiumfor the Barcode of Life (CBOL)

2007-11-07 The 1st KRIBB Poster Festival

2007-11-01The 6th Osong International Bio-Symposium (Osong, Korea)

2007-10-08 Participating in the HUPO 6th AnnualWorld Congress (Seoul, Korea)

2007-10-05KRIBB-ITRI (Taiwan) Joint Symposium

2007-10-04Daejeon-KRIBB-FHCRC Joint Symposium

2007-09-10Visit by Merck representatives

2007-07-09Visiting of INBio delegation, Costa Rica

2007-07-03 Visit by the President of the ArabScience & Technology Foundation (ASTF)

2007-06-15 Visit by the Vice Chancellor of theUniversity of Western Australia

2007-06-14 Signing of an MOU with Pfizer Inc.

2007-05-31 KRIBB-BIOTEC (Thailand) Joint Symposium

2007-05-07 Participating in ‘BIO 2007’ (Boston), theworld’s greatest Bio exposition

2007-03-09 Visit by the President of CNRS, France

2007-02-12Signing of an MOU with the University ofValencia, Spain

Location

DaejeonMain Campus

OchangCampus

JeonbukBranch Institute

Korea Research Institute of Bioscience and Biotechnology concentrates on the promotion of human welfare and national prosperity

Published by KRIBB

Edited by Sang Ki RheeDesigned by Design-Thecopyright @ 2007 KRIBB

All rights reserved. No part of this publication may be reproduced,

stored in a retrieval system or transmitted in any form or

by any means, electronic, mechanical, photocopying, recording or otherwise,

without the permission of the copyright holder.

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