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INTRODUCTION TO PROTEOMICS COURSE OUTLINE : ABOUT INSTRUCTOR : COURSE PLAN : This course introduces to the basic biology of proteins and the new advanced science called as proteomics which aims to look into the protein properties from a global perspective, i.e., not undertaking one protein at a time, but an entire set of proteins in the milieu. The course will cover in detail the two major aspects of proteomics i.e., Gel-based proteomics and Mass spectrometry-based proteomics. The gel-based module will cover different techniques like SDS-PAGE, 2-DE, 2D-DIGE etc. These techniques had a major contribution in transition from protein chemistry to proteomics. Mass spectrometry, on the other hand, is an advanced analytical technique for accurate mass measurement. In this module, we will discuss the basics of mass spectrometry, sample preparations, liquid chromatography, hybrid mass spectrometers and quantitative proteomics techniques such as iTRAQ, SILAC and TMT using mass spectrometry. The course will also provide the basic knowledge about sample preparation, mass spectrometry workflow, different chromatography technologies and quantitative proteomics. Dr. Sanjeeva Srivastava is the Group Leader for the Proteomics Laboratory at the Indian Institute of Technology Bombay India (IITB). He obtained his Ph.D. from the University of Alberta and post-doc from the Harvard Medical School in the area of proteomics, stress physiology and has specialized expertise in applications of data enabled sciences in global health, developing country and resource limited settings. Week 01 : Week 02 : Week 03 : Week 04 : Week 05 : Week 06 : Week 07 : Week 08 : PROF. SANJEEVA SRIVASTAVA Department of Biosciences & Bioengineering IIT Bombay Basics of Proteins and Proteomics Gel-based proteomics Two-dimensional gel electrophoresis (2-DE) Difference in gel electrophoresis (DIGE) & Systems Biology Basics of mass spectrometry Basics of mass spectrometry and sample preparation Quantitative proteomics Advancement in Proteomics INTENDED AUDIENCE PRE-REQUISITES B.Sc., M.Sc. and MS Any B.Sc. or M.Sc.The target audiences of this course are required to have a basic introduction to biology. : : TYPE OF COURSE COURSE DURATION EXAM DATE Rerun | Elective | UG/PG 8 weeks (17 Aug’20 - 09 Oct’20) 17 Oct 2020 : : :

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INTRODUCTION TO PROTEOMICS

COURSE OUTLINE :

ABOUT INSTRUCTOR :

COURSE PLAN :

This course introduces to the basic biology of proteins and the new advanced science called as proteomics which aims to look into the protein properties from a global perspective, i.e., not undertaking one protein at a time, but an entire set of proteins in the milieu. The course will cover in detail the two major aspects of proteomics i.e., Gel-based proteomics and Mass spectrometry-based proteomics. The gel-based module will cover di�erent techniques like SDS-PAGE, 2-DE, 2D-DIGE etc. These techniques had a major contribution in transition from protein chemistry to proteomics. Mass spectrometry, on the other hand, is an advanced analytical technique for accurate massmeasurement. In this module, we will discuss the basics of mass spectrometry, sample preparations, liquidchromatography, hybrid mass spectrometers and quantitative proteomics techniques such as iTRAQ, SILAC and TMT using mass spectrometry. The course will also provide the basic knowledge about sample preparation, mass spectrometry work�ow, di�erent chromatography technologies and quantitative proteomics.

Dr. Sanjeeva Srivastava is the Group Leader for the Proteomics Laboratory at the Indian Institute of Technology Bombay India (IITB). He obtained his Ph.D. from the University of Alberta and post-doc from the Harvard Medical School in the area of proteomics, stress physiology and has specialized expertise in applications of data enabled sciences in global health, developing country and resource limited settings.

Week 01 :Week 02 :Week 03 :Week 04 :Week 05 :Week 06 :Week 07 :Week 08 :

PROF. SANJEEVA SRIVASTAVADepartment of Biosciences & BioengineeringIIT Bombay

Basics of Proteins and ProteomicsGel-based proteomicsTwo-dimensional gel electrophoresis (2-DE) Di�erence in gel electrophoresis (DIGE) & Systems BiologyBasics of mass spectrometry Basics of mass spectrometry and sample preparation Quantitative proteomicsAdvancement in Proteomics

INTENDED AUDIENCEPRE-REQUISITES

B.Sc., M.Sc. and MSAny B.Sc. or M.Sc.The target audiences of this course are required to have a basicintroduction to biology.

TYPE OF COURSECOURSE DURATIONEXAM DATE

Rerun | Elective | UG/PG8 weeks (17 Aug’20 - 09 Oct’20) 17 Oct 2020

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