introduction our past ancestors stood on the face of this earth with the belief that it was a...

70
Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view, was the center of the universe. People believed humans were totally unrelated to other animal life here on Earth; that humans were placed on this earth to rule over and make use of the boundless resources, put here for the sole benefit of humans. Water, land, air, minerals, forests, fish, and all other resources, both renewable and non- renewable, were all considered to be in endless supply.

Upload: louise-webster

Post on 25-Dec-2015

215 views

Category:

Documents


0 download

TRANSCRIPT

Page 1: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Introduction

• Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world.

• The Earth, in their view, was the center of the universe. • People believed humans were totally unrelated to other

animal life here on Earth; that humans were placed on this earth to rule over and make use of the boundless resources, put here for the sole benefit of humans. Water, land, air, minerals, forests, fish, and all other resources, both renewable and non-renewable, were all considered to be in endless supply.

Page 2: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,
Page 3: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,
Page 4: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Paradigm

The way that humans view the world is known as a paradigm.

• "The earth and all things on it exist for the sole benefit of humans" was an old world paradigm. This paradigm was the reason that western civilization was created on the premise of unlimited exploitation of the earth!

• Today it is clearly understood by most people that the early views were wrong.

• The old paradigms have been replaced by new paradigms.

Page 5: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Paradigm Shift

• Changes in paradigms are known as paradigm shifts. Humans no longer regard the Earth as the centre of the universe.

• Humans now recognize that the Earth is a sphere rather than flat.

• The Earth's resources are not in endless supply for our plunder.

• We are the caretakers of this world and we must take responsibility for its sustainability.

• These are all examples of paradigm shifts. • The paradigms of modern man differ from the paradigms

of our forefathers.

Page 6: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Sustainability

• The modern paradigm views the Earth as a sustainable system provided that renewable resources are not used at a faster rate than they are replaced or recycled.

• The term sustainability means that the system can meet the needs not only of our present human population, but also those of the future.

Page 7: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Introduction to Ecology

• Is the scientific study of the interactions of organisms and their environment

• Interactions of organisms and their environment includes how the environment affects the organism and how the organism affects the environment

• Ecosystem – is a community of organisms and their physical environment

Page 8: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

                                                          

• An ecosystem is a community made up of animals, plants, and micro-organisms, together with the environment where they live.

• Members of the community depend on each other and their environment for food and shelter.

• Ecosystems have four main components: air, water, land and living creatures.

• Every ecosystem on Earth depends on water, of varying amounts, for its survival.

Page 9: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,
Page 10: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,
Page 11: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Abiotic Factors

• Space

• Temperature

• Oxygen

• Sunlight

• Water

• Inorganic and organic soil nutrients

Page 12: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Biotic Factors

• Detritus

• Disease

• Predator\Prey interactions

• Competition

Page 13: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Symbiotic Relationships

• Are biotic relationships in which two or more organisms live in close association with each other to the benefit of at least one

• Five types– Mutualism – results in mutual benefit to both organisms– Commensalism – one organism benefits the other neither

benefits nor is harmed– Parasitism – one organism benefits and the other one is

harmed– Parisitoidism – one benefits but the other is eventually killed– Predation – the interaction is beneficial to one and the other is

killed

Page 14: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Mutualism

Page 15: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Predation

Page 16: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Commensalism

Page 17: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Parasitism

Page 18: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Parasitoids

Page 19: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Trophic Structure• Each step in the food chain is called a trophic level• Refers to the feeding relationships within the ecosystem• Five levels

1. Primary producers – also called autotrophs – they produce their own food

• Consumers – heterotrophs – organisms that eat other organisms• 4 types of consumers

– Herbivores – eat plants– Carnivores – eat animals– Omnivores – eat both plants and animals– Saprobes – decomposes – eat dead plants and animals

2. Primary consumers – feed directly on plants3. Secondary consumers –feed on primary consumers4. Tertiary consumers – top consumer because it is at top of food chain5. Decomposers

Page 20: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Trophic Structure (cont’d)

• Feeding relationships within an ecosystem are generally called a food web

• The food web consists of all the possible food chains within the ecosystem

Page 21: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,
Page 22: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,
Page 23: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Your Turn

• Read pg. 8 – 13 – Questions Pg. 13 # 1,2,3,4(a)(c)

• Read pg. 22 – 23 – Questions Pg. 23 # 1 – 6

• Read pg. 34 – 39 – Questions Pg. 39 # 1 -7

• Pg. 46 # 6

Page 24: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Succession• Ecological succession refers to the series of ecological changes

that every community undergoes over long periods of time. • succession begins with relatively few pioneering plants• The plant life serves as food, and often shelter for the animal life that

can survive in that environment. • succession in the plant life is paralleled by a succession in animal life. • as a result succession, a primitive community develops. • organisms that make up the primitive community gradually change

the environmental conditions so each successive community paves the way for the next.

• Each successive community becomes more complex until it becomes a final, sustainable, stable community called a climax community.

• In an ecosystem with a climax community, the conditions continue to be suitable for all the members of the community.

• The climax community is the final stage of ecological succession.

Page 25: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Primary Succession• primary succession occurs in an area where there is no soil or

previous forms of life.• occurs in an area such as a freshly cooled lava field, or a newly

formed sand dune. • on land, primary succession is a very slow process because it

begins with the formation of soil. • The soil forms as a result of weathering and the action of pioneer

organisms. • Large rocks are broken down into smaller pieces and eventually

bacteria, fungi, and lichens inhabit the area. T• these organisms are known as pioneer organisms because they

are the first type of life to inhabit the region.

Page 26: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

• The pioneer organisms add organic matter to the primitive soil, changing the conditions of the microenvironment so that mosses, ferns, and other primitive plants begin to take over.

• Grasses may eventually replace the more primitive plants and when they die, they make the soil even richer.

• Shrubs grow and shade the grass causing it to die. • Then trees may grow and shade out the shrubs. • Seedlings of other trees may grow well in the shade. • In this way, one community of trees will be succeeded by

another community with different trees.

Page 27: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Secondary Succession

• Secondary succession occurs in an area in which an existing community has been partially destroyed and its balance upset, either by natural causes, such as fire, or as a result of human activity, such as the cutting of a forest, or abandoning a farm.

• The major difference between primary and secondary succession in a terrestrial environment is that in secondary succession, soil already exists. S

• seeds of plants will begin to grow. • Those that do grow will come from dormant seed already

in the soil, or will come from plants in communities nearby.

Page 28: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

• The seeds will establish a community but succession will eventually result in a climax community that is often the same as would normally be found in the typical climax community in the region.

• An abandoned farm may become a forest given enough time.

• The final climax community is generally the same as the climax community that surrounds the disturbed area.

• The series of stages leading to the climax community will not be the same as for a primary succession that created the original climax community.

Page 29: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Factors that contribute to ecological succession:

• The type of climax community that is established will depending on the environmental conditions of the area.

• The most important environmental conditions that affect succession include:– climate (temperature, precipitation, and availability of

sunlight), – soil (salinity, fertility, moisture, texture, etc.), – and geographical features (latitude, altitude, and

proximity to mountain ranges or large bodies of water).

Page 30: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

• Every ecosystem exists because there is a balance between its members (producers, herbivores, omnivores, predators, scavengers, parasites, competitors, decomposers, etc.) and its abiotic environment (climate, soil, availability of sunlight, pH, oxygen levels, salinity, etc.).

• It is this balance between the biotic and abiotic factors that creates the stability of the ecosystem.

Page 31: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

• In general, the greater the biodiversity, the greater the stability.

Page 32: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Your Turn

• Search the web to find at least one example to illustrate either primary succession or secondary succession. Prepare a presentation, including a sequence of diagrams or images, to describe the changes that occur during succession and lead to the formation of a climax community. Your series of diagrams or images should identify the different types of plant and animal life found at each stage in the process, and clearly identify the type of climax community formed at the end of the process of succession. You presentation should include answers to each of the following questions:

• What is succession? • What factors contribute to succession? • What are the characteristics of a climax community?

Page 33: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Energy Flow in Ecosystems

• Vast majority of all life on earth depends on sunlight as its source of energy

• Actual amount of light that reaches the surface of the earth id affected by the albedo effect

• The albedo effect is a measure of the amount of light reflected from an object

Page 34: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,
Page 35: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Energy Flow in Ecosystems (cont’d)

Photosynthesis is a biological process whereby the suns energy is used by green plants to manufacture sugar – oxygen is also produced

Carbon dioxide + water + sunlight = sugar + oxygen

The sugar that is produced serves as the source of energy for nearly all life.

The passage of food from producer to various consumers is called a food chain

Page 36: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Energy Flow in Ecosystems (cont’d)

• The transfer of energy through an ecosystem is never 100% efficient since each organism must use some of the energy for its own existence.

Page 37: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Energy Flow in Ecosystems (cont’d)

• The energy budget or the amount of energy that is available to an ecosystem depends on its producers

• Ecologists have adapted the 10 % rule, put simple “ only 10% of the available energy at one trophic level is passed on to the next level”

Page 38: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Energy Flow in Ecosystems (cont’d)

Example

Corn – primary producer -10 000 JMouse – primary consumer - 1 000 JSnake – secondary consumer - 100 JHawk – tertiary consumer - 10 J

The idea that each higher trophic level has less energy available is known as the pyramid of energy

Page 39: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Energy Flow in Ecosystems (cont’d)

Corn

Mouse

Snake

Hawk

Pyramid of Energy

Page 40: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Pyramid of Biomass

• Since the amount of water present within the tissues of different organisms varies, biologists use the dry mass of the organism for comparison since it is believed that dry mass more closely reflects the actual amount of "living matter" in the organism.

• The dry mass is known as biomass. The availability of energy will also affect the number of organisms and the mass of the organisms at each trophic level.

• In general, the higher the amount of available energy the higher is the biomass (dry mass) at each trophic level.

• The pyramid of biomass is a graphical representation of the total biomass of all the members of each trophic level.

• Generally the pyramid of biomass has the same shape as the pyramid of energy.

Page 41: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Pyramid of Numbers• The pyramid of number is the third type of graphical

representation used by biologists to study ecosystems. • The pyramid of number is often similar in shape to the pyramid

of energy or biomass, but there are exceptions. • Consider a single spruce tree in a boreal forest (biomass = 100

kg) which is infested by 100,000 spruce bud worms (total biomass = 10 kg), which are in turn eaten by 5 insect eating birds (total biomass = 1 kg).

• The pyramid of biomass would appear normal (base representing 100 kg, middle piece representing 10 kg, and a top piece representing 1 kg).

• The pyramid of number for this example will not look normal. • The pyramid of number would have a very small base

representing the producer (1 tree), a very large herbivore level (100,000 spruce bud worms), followed on top by a small predator level (5 birds).

Page 43: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Your Turn

• Pg. 39 # 9 - 14

Page 44: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

# 9

• Less than 10% of the energy in a produces is transferred to a primary consumer because it uses the rest to carry on photosynthesis, grow new tissue, reproduce, etc.

Page 45: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

# 10

• The mouse uses some of the energy ot acquired from its plant food in metabolism, growth, locomotion, etc

Page 46: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

# 11

• To construct a pyramid of numbers, you would have to count all of the organisms in the population making up the food chain

Page 47: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

# 12

• A pyramid of numbers does not show energy flow because of the potential disparity in the sizes of the organisms.

• It is not a good indicator of the health of an ecosystem

Page 48: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

# 13

• A pyramid of energy for a grassland in winter would show considerably less energy at the producer level than a grassland in the summer. The grassland receives less solar energy in the winter than in the summer.

• Day is shorter and covered by snow

Page 49: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

# 14

• The pyramid of numbers for a deciduous forest has a small base because it doesn’t take many trees to produce a large biomass.

• The large biomass is shown by the large base of the pyramid of biomass.

Page 50: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,
Page 51: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

• Niche, refers to the role that a species plays within its ecosystem. In balanced ecosystems, each species occupies its own niche. The niche is like the organism's profession - what it does to survive.

• Habitat refers to the place were an organism lives. The habitat of a species is different than its niche, it is the particular part of the environment in which it lives. The habitat of an organism is part of its niche. The organism's habitat is its address - where it lives.

Page 52: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Competition

• Competition between organisms exists in every ecosystem.

• Organisms are forced to compete against their own species and also different species in order to survive.

• The stronger and more fit organisms have a better chance of surviving.

• Competition arises when organisms have requirements in common and they must compete to meet their own needs.

• The more needs organisms have in common, the more intense the competition.

• When the resources that are being competed for become scarce the competition becomes more intense, and eventually one of the species becomes eliminated.

Page 53: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

• Competition between the same species is called intraspecific competition. – Ex. birds of the same species compete for

the best nesting grounds.

• Competition between different species is called interspecific competition. – Ex. the lion and the hyena both compete for

zebra.

Page 54: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Your Turn

• Pg. 44 # 1 - 3

Page 55: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Pests and Pesticides

• Pests are living organisms that are not wanted around us.

• Examples of pests include dandelions ,rodents or insects

• A pest is any organism that man believes is undesirable, has a negative impact on the human environment, or is in competition with human use of a resource, either natural, or cultivated.

Page 56: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Early Pesticide Use:

• Early pesticides included the use of toxic inorganic metallic salts such as copper sulfate, lead salts, arsenic, or mercury. 

• These substances were generally effective against the intended pest, but also created some environmental problems because they also killed other beneficial organisms, and polluted water and soil resources used by man.

• Most early pesticides were non-biodegradable (meaning that they were not broken down within the ecosystem).

• As a result, these early pesticides began to accumulate in the environment, contaminating water and soil resources, eventually poisoning humans.

Page 57: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Modern Pesticides:

• chemists began to develop organic pesticides that were designed to be less toxic to man and more specific toward the intended pests.

• It was soon discovered that the organic pesticides also caused unexpected environmental effects.

• Some of these pesticides were fat soluble. • This characteristic lead to a problem known as

bioaccumulation. • As each organism feeds on one lower in the food chain,

the fat soluble pesticide began to be concentrated in ever higher amounts as one moved toward the top of the food pyramid.

Page 58: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

• Since every organism eats far more than its own body mass in food, the tiny amounts found in each organism in the lower levels of the food web began to accumulate in greater concentrations in species located at higher trophic levels.

• One example of this problem is illustrated by the damage done to predatory birds as a result of bioaccumulation of DDT.

• As a result of this problem DDT has been banned from use in North America.

Page 59: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Your Turn

• Read pg. 52 – 58

• Answer questions # 1 - 10

Page 60: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Biochemical Cycles

• oxygen, carbon, hydrogen and nitrogen make up the vast majority of living tissue.

• These four elements are recycled between living organisms and the soil, water and atmosphere

• These elements are first taken up by plants, converted into food, passed through the food web, consumers and decomposers, then returned to the environment in a continuous recycling of materials.

• If recycling of these materials did not occur, life could not exist.

Page 61: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

• continuation of life depends on the continued recycling • Some elements (carbon, oxygen, sulfur, nitrogen) are

found in gaseous forms and their cycles involve the atmosphere. As a result they have a global nature.

• some of the elements may have a short term cycle such as when carbon is transferred from animals to plants in the form of carbon dioxide and a long term cycle such as the transfer of carbon from a fossil fuel to a plant following combustion.

• elements are cycled between the living organisms and the environment (both long and short term).

• It is a combination of biological and geological processes that drives chemical recycling

Page 62: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

• The elements are cycled between the living organisms and the environment (both long and short term). It is a combination of biological and geological processes that drives chemical recycling.

Page 63: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Biological Processes

– respiration– decomposition – excretion – photosynthesis – and assimilation

Page 64: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Geological Processes

– fossilization– erosion– combustion of fossil fuels (peat, oil, coal)– weathering – formation of sedimentary rock

Page 65: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Carbon Cycle

Page 66: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Your Turn

• Read 2.5 "The Carbon Cycle" on pages 62 - 65. Answer questions 1 - 7 from "Understanding Concepts" and "Making Connections" on page 65.

Page 68: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Your Turn

• Read 2.6 "The Nitrogen Cycle" on pages 66 - 67. Answer questions 1 - 6, 8 - 11, and 13 - 14 (omit questions 7 and 12) from "Understanding Concepts," "Making Connections," and "Reflecting" on page 69.

Page 69: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

Surface area to volume

• in order to survive, cells must constantly interact with their surrounding environment.

• Gases and food molecules dissolved in water must be absorbed and waste products must be eliminated.

• For most cells, this passage of all materials in and out of the cell must occur through the plasma membrane.

• Each internal region of the cell has to be served by part of the cell surface.

Page 70: Introduction Our past ancestors stood on the face of this Earth with the belief that it was a tremendously large and flat world. The Earth, in their view,

• As a cell grows bigger, its internal volume enlarges and the cell membrane expands.

• Unfortunately, the volume increases more rapidly than does the surface area, and so the relative amount of surface area available to pass materials to a unit volume of the cell steadily shrinks.

• At some point, there is just enough surface available to service all the interior; if it is to survive, the cell must stop growing.

• If the cell grows beyond a certain limit, not enough material will be able to cross the membrane fast enough to accommodate the increased cellular volume.