introduction - microchem · introduction: microchem lab services (pty) ltd is an independent and...

Introduction: Microchem Lab Services (Pty) Ltd is an independent and authoritative provider of a comprehensive range of chemical and microbiological analyses and related services to the food and non-food industries. Our aim is to offer comprehensive chemical, nutritional, environmental, microbiological, and food borne pathogen analysis to clients. Microchem was established by Raymond Hartley in 2007 when he bought the Woolworths in-house laboratory. In 2009 Microchem acquired Consulting Microbiological Laboratory (CML) in Gauteng. CML was a well known microbiological laboratory, the acquisition allows for greater opportunities to service Microchem’s Gauteng clients who require microbiological services. Our Services:

Routine food microbiology analyses

Food borne pathogen analyses

Water microbiological analyses

Product shelf-life analyses

Foreign object inspections

Hygiene inspections and reports

Microbiological swabbing

Food chemistry

Nutritional analyses

Trace elemental and heavy metal analyses

Food colorants (Azo Dyes)

Vitamin analyses

Water chemical analyses

Melamine analyses

Pesticide analyses Consulting services: Our specialists provide consultation on the assessments and advice on environmental hygiene management programmes, interpretation of analytical results and advice on nutritional analyses, as well as assistance with pathogen management in production facilities. Quality: Microchem is a South African National Accreditation System (SANAS) accredited laboratory that uses a quality system which meets national and international criteria (EN45001, ISO 17025). The analytical methods employed are based on nationally and internationally accepted procedures.

Facilities: To meet your analytical requirements our modern facilities include centrally situated laboratories with over 1000 square meters of laboratory space for adequate sample reception, analyses and storage. Microchem continuously invests in the latest technology, Our state of the art technology includes GC, GC/MS, GS-MS/MS, ICP-OES, HPLC, LC-MS/MS, real time PCR, Tempo, Vidas and LIMS. These instruments have greatly broadened the range of analyses, and enable us to test for substantially lower levels of detection, and allows for the analysis of whole new classes of compounds. Personnel: Our laboratories are staffed with advanced degree chemist, microbiologists, biotechnologists, food technologists, and highly trained analysts to provide an exemplary and personalized service. The personnel’s strong, multi-skilled technical backgrounds and experience in a variety of analytical specialties ensure that our laboratory stays in the forefront of technology to provide superior results. Why use Microchem? We meet the needs of our clients by providing:

Quality assured results (SANAS accredited)

Excellent service

The convenience of one-stop lab solution

Rapid turnaround times

Competitive prices Contact Information: Cape Town (Head Office)

1st Floor Fairweather House,

176 Sir Lowry Road

Woodstock, Cape Town, South Africa, 8001

Tel: +27 (21) 465-6996

Managing Director: Raymond Hartley

[email protected]

Gauteng Micro Laboratory

41 Golden Drive,

Morehill, Benoni, South Africa, 1501

Tel: +27 (11) 425-3775

Please visit our website for additional information: www.microchem.co.za

mailto:[email protected]

http://www.microchem.co.za/

Tests:

ANALYSES SANAS Accred

ited

Lead times (Days)

Method used Instrument LOD Sample amount required

Nutritional Analyses 10 to 14 min 350g

% Moisture Yes AOAC 950.46 Drying in air

oven 0.0024g

% Ash Yes AOAC 923.03 Ash by

Furnace 0.0022g

% Protein (% Nitrogen included in protein analysis)

Yes AOAC 968.06 Leco Protein

analyser 0.07%

% Total fat and Fatty Acid Profile (Including the following):

Yes AOAC 996.06 GC 0.00844g/100g

% Saturated Fat Yes AOAC 996.06 GC -

% Mono unsaturated fat Yes AOAC 996.06 GC -

% Poly unsaturated fat Yes AOAC 996.06 GC -

% Trans fat Yes AOAC 996.06 GC -

% Omega 3 (EPH/DHA), 6 & 9 Yes AOAC 996.06 GC -

mg Cholesterol Yes AOAC 976.26 GC 1.1455mg/100g

% Glycaemic carbohydrates Yes -

% Total sugars: (Used for Glycaemic Carbohydrates)

Yes AOAC 982.1 (amended)

GC -

% Fructose Yes AOAC 982.1 (amended)

GC 0.009mg/g

% Glucose Yes AOAC 982.1 (amended)

GC 0.003mg/g

% Sucrose Yes AOAC 982.1 (amended)

GC 0.018mg/g

% Maltose Yes AOAC 982.1 (amended)

GC 0.020mg/g

% Galactose Yes AOAC 982.1 (amended)

GC 0.006mg/g

% Trehalose Yes AOAC 982.1 (amended)

GC 0.012mg/g

% Lactose Yes AOAC 982.1 (amended)

GC 0.038mg/g

% Starch (Used for Glycaemic Carbohydrates)

Yes AOAC 996.11 HPLC 0.1457mg/g

% Total dietary Fibre Yes AOAC 991.43 Gravimetric -

mg Sodium Yes AOAC 984.27

(amended) ICP-OES 0.0811ppm

KJ/100g Energy (by calculation) Yes Calculated

% Carbohydrates (Calculated) Yes Calculated

Chemical Analyses

% Total dietary Fibre (Part of full nutritional analyses)

Yes 10 to 14 AOAC 991.43 Gravimetric -

& Total dietary fibre (Including fat,moisture and sugar)

Yes 10 to 14 AOAC 991.43 Gravimetric -

% Soluble and Insoluble dietary fibre No 10 to 14 AOAC 991.43 Gravimetric -

pH No 2 pH meter -

Brix No 2

Handheld refractometer

-

% Acidity (total acidity) Yes 5 to 10 AOAC 942.15 Titration -

% Salt (as NaCL) Yes 5 to 10 Metrohm

application Autotitrator 0.06% NaCL

Peroxide value (milli equivalence) on fat or oil

No 5 to 10 AOAC 965.33 Titration -

Peroxide value (food products containing > 20% fat


% Free fatty acids- (By titration only on fats or oils)


& Free fatty acids (Food products containing > 20% fat)


ppm Sulphur dioxide No 14-20 AOAC 962.16 -

Vitamins Analyses min 100g

Vitamin A- Retinol Yes 5 to 10 AACC 86-06 HPLC 0.0121mg/g

Vitamin E- Tocopherol Yes 5 to 10 AACC 86-06 HPLC 0.0523mg/g

Vitamin C- Ascorbic acid Yes 5 to 10 AOAC 984.26 HPLC 0.4312ug/g

Vitamin B1- Thiamine Yes 5 to 10 AOAC 95.7.17 HPLC 0.021ug/g

Vitamin B2- Riboflavin Yes 5 to 10 AOAC 95.7.17 HPLC 0.050ug/g

Vitamin B3- Niacin Yes 5 to 10 AOAC 95.7.17 HPLC 0.500ug/g

Vitamin B5- Pantothenic acid No 5 to 10 - LC-MS -

Vitamin B6- Pyridoxine Yes 5 to 10 AOAC 95.7.17 HPLC 0.057ug/g

Vitamin B7- Biotin No - LC-MS -

Vitamin B9- Folic acid No - LC-MS -

Elemental Analyses min 100g

Aluminium (ICP-OES analysis) No 5 to 10 AOAC 984.27

(amended) ICP-OES -

Arsenic (ICP-OES analysis) Yes 5 to 10 AOAC 984.27


Barium (ICP-OES analysis) No 5 to 10 AOAC 984.27

(amended) ICP-OES -

Bismuth (ICP-OES analysis) No 5 to 10 AOAC 984.27

(amended) ICP-OES -

Boron (ICP-OES analysis) No 5 to 10 AOAC 984.27

(amended) ICP-OES -

Cadmium (ICP-OES analysis) Yes 5 to 10 AOAC 984.27


Calcium (ICP-OES analysis) Yes 5 to 10 AOAC 984.27


Chromium (ICP-OES analysis) No 5 to 10 AOAC 984.27

(amended) ICP-OES -

Cobalt (ICP-OES analysis) No 5 to 10 AOAC 984.27

(amended) ICP-OES -

Copper (ICP-OES analysis) Yes 5 to 10 AOAC 984.27


Gallium (ICP-OES analysis) No 5 to 10 AOAC 984.27

(amended) ICP-OES -

Indium (ICP-OES analysis) No 5 to 10 AOAC 984.27

(amended) ICP-OES -

Iron (ICP-OES analysis) Yes 5 to 10 AOAC 984.27


Lead (ICP-OES analysis) Yes 5 to 10 AOAC 984.27


Lithium (ICP-OES analysis) No 5 to 10 AOAC 984.27

(amended) ICP-OES -

Magnesium (ICP-OES analysis) Yes 5 to 10 AOAC 984.27


Manganese (ICP-OES analysis) Yes 5 to 10 AOAC 984.27


Mercury (ICP-OES analysis) Yes 5 to 10 AOAC 984.27


Molybdenum (ICP-OES analysis) No 5 to 10 AOAC 984.27

(amended) ICP-OES -

Nickel (ICP-OES analysis) No 5 to 10 AOAC 984.27

(amended) ICP-OES -

Phosphorus (ICP-OES analysis) No 5 to 10 AOAC 984.27


Potassium (ICP-OES analysis) Yes 5 to 10 AOAC 984.27


Selenium (ICP-OES analysis) No 5 to 10 AOAC 984.27

(amended) ICP-OES -

Silver (ICP-OES analysis) No 5 to 10 AOAC 984.27

(amended) ICP-OES -

Sodium (ICP-OES analysis) Yes 5 to 10 AOAC 984.27


Strontium (ICP-OES analysis) No 5 to 10 AOAC 984.27

(amended) ICP-OES -

Sulphur (ICP-OES analysis) No 5 to 10 AOAC 984.27

(amended) ICP-OES -

Thallium (ICP-OES analysis) No 5 to 10 AOAC 984.27

(amended) ICP-OES -

Tin (ICP-OES analysis) No 5 to 10

AOAC 984.27 (amended)

ICP-OES -

Zinc (ICP-OES analysis) Yes 5 to 10 AOAC 984.27


Water Analyses - SANS 241 10 to 14 min 1L of

water

Aluminium No 5 to 10 AOAC 984.27

(amended) ICP-OES -

Ammonia as N No 5 to 10 Spectrophoto

meter -

Arsenic as As Yes 5 to 10 AOAC 984.27


Cadmium as Cd Yes 5 to 10 AOAC 984.27


Cloride as Cl No 5 to 10 Spectrophoto

meter -

Cobalt as Co No 5 to 10 AOAC 984.27

(amended) ICP-OES -

Conductivity No 5 to 10 Conductivity

meter -

Copper as Cu Yes 5 to 10 AOAC 984.27


Cyanide (recoverable) as CN No 5 to 10 Spectrophoto

meter -

Flouride as F No 5 to 10 Spectrophoto

meter -

Iron as Fe Yes 5 to 10 AOAC 984.27


Lead as Pb Yes 5 to 10

AOAC 984.27 (amended)

ICP-OES 0.0523ppm

Managanese as Mn Yes 5 to 10 AOAC 984.27


Mercury as Hg Yes 5 to 10 AOAC 984.27


Nickel as Ni No 5 to 10 AOAC 984.27

(amended) ICP-OES -

Nitrate as N No 5 to 10 Spectrophoto

meter -

Nitrite as N No 5 to 10 Spectrophoto

meter -

pH No 5 to 10 pH meter -

Selenium as Se No 5 to 10 AOAC 984.27

(amended) ICP-OES -

Sodium as Na Yes 5 to 10 AOAC 984.27


Total chromium as Cr No 5 to 10 AOAC 984.27

(amended) ICP-OES -

Total dissolved solids No 5 to 10 Conductivity

meter -

Vanadium as V No 5 to 10 AOAC 984.27

(amended) ICP-OES -

Zinc as Zn Yes 5 to 10 AOAC 984.27


Strontium No 5 to 10 AOAC 984.54 ICP-OES -

Thallium No 5 to 10 AOAC 984.55 ICP-OES -

Other Chemical Analyses

Azo dyes: which includes Yes 10-15 In house developed LC-MS - min 100g

Sudan 1 Yes 10-15 LC-MS -




Para Red Yes 10-15 LC-MS -

Methyl Yellow Yes 10-15 LC-MS -

* Additional cost if Erythorosine present in sample

No

Melamine screening Yes 10-15 In house developed LC-MS - min 100g

Melamine quantification No 10-15 LC-MS -

Sunscreen Analysis No 10-15 In house developed

Zinc oxide No 10-15

CAPE TOWN MICROBIOLOGICAL ANALYSIS

SANAS Accred

ited

Lead times Days

Method used Instrument LOD Sample Amount

Routine Micro min 100g

TVC (Total Viable Count) Yes 3 MFHPB-18 <10 CFUs/g

Enterobacteriaceae Yes 2 ISO 7402 <10 CFUs/g

Coliforms Yes 2

ISO 4832/ISO 5541/1

<10 CFUs/g

Yeast and Mould Yes 6

Oxoid-Acidified PDA

<10 CFUs/g

Lactic acid bacteria Yes 4 ISO 15214:1998 <10 CFUs/g

Osmophillic yeasts and moulds Yes 6

Oxoid-Modified Wort agar

<10 CFUs/g

Pseudomonas spp. Yes 3

CCFRA Method 9.1: 1995

<10 CFUs/g

Commercial sterility No 6 MFHPB-01 <10 CFUs/g

Species ID: Gram negative bacteria No 3 Oxoid n/a

Sporeformers: Aerobic No 3 MFLP- 44 <10 CFUs/g

Sporeformers: Anaerobic No 3 MFLP- 44 <10 CFUs/g

Pathogens 25g/patho

gen

E. coli (confirmed) Yes 2 Oxoid-TBX agar <10 CFUs/g

Staphylococcus aureus (confirmed) Yes 2 ISO 6888-2 <10 CFUs/g

Listeria monocytogens (detection) Yes 3 AFNOR BRD: 07/04

-09/98 <10 CFUs/25g

Listeria monocytogens (confirmation)

Yes 2 AFNOR BRD: 07/04

-09/98 n/a

Listeria monocytogens (biochemical confirmation)


-09/98 n/a

Listeria monocytogens count Yes 2 AFNOR BRD: 07/05

-09/01 <10 CFUs/25g

E. coli 0157 (detection) No 3 Oxoid-SMAC agar

with BCIG <10 CFUs/25g

E. coli 0157 (confirmation) No 2 Oxoid n/a

Salmonella (detection) Yes 4 AFNOR BRD 07/11–

12/05 <10 CFUs/25g

Salmonella (confirmation) Yes 3 AFNOR BRD 07/11–

12/05 n/a

Bacillus cereus (presumptive count) No 2 Oxoid-Brilliance <10 CFUs/g

Clostridium perfringens (detection) No 2 Oxoid-OPSP Agar <10 CFUs/g

Clostridium perfringens (confirmation)

No 2 Oxoid

<10 CFUs/g

Vibrio spp (confirmed) No 7 Lab developed <10 CFUs/25g

Water analyses

TVC (water) Yes 4 Oxoid-R2A <1 CFUs/ml 50ml

E. coli (water) Yes 2 AFNOR BRD 07/7-

12/04

<1 CFU/ volume filtered

250ml

Coliforms (water) Yes 2 AFNOR BRD 07/8 -

12/04


250ml

Pseudomonas spp (water) Yes 3 CCFRA Method 9.1:

1995


250ml

Enviromental Swabs

TVC Yes 3 MFHPB-18

<10 CFUs/area

swabbed 1 swab

Enterobacteriaceae Yes 2 ISO 7402

<10 CFUs/area

swabbed

Coliforms Yes 2 ISO 4832 <10 CFUs/area

swabbed

E. coli Yes 2 Oxoid

<10 CFUs/area

swabbed

Staphylococcus aureus Yes 2 ISO 6888-2

<10 CFUs/area

swabbed

Yeast and Mould Yes 6 Oxoid-Acidified

PDA

<10 CFUs/area swabbed

Listeria monocytogensswab (detection)


-09/98


1-3 swabs

Listeria monocytogenes sponge (detection)


-09/98


1 sponge

Listeria monocytogens(confirmation)


-09/98 n/a n/a

Airplates

TVC No 3 MFHPB-18 n/a 1 plate

Yeast and Mould No 6 Oxoid-Acidified

PDA n/a 1 plate

Other tests offered

Foreign Object Identification No volume

dependant Visual/photographi

c Stereomicrosc

ope n/a n/a

Water Activity No volume

dependant Novasina

Novasina Aw unit

100g

GAUTENG MICROBIOLOGICAL ANALYSIS

SANAS Accred

ited

Lead times Days

Method used Instrument LOD Sample Amount

Routine Micro n/a Minimum

100g

TPC (Total Plate Count) Yes

3 AOAC Official

Method 996.23 <10 CFUs/g

Anaerobic Total Plate Count No

3 CCFRA Method

1.1.1:2003 <10 CFUs/g

Coliforms Yes

2 BIORAD 07/8 -

12/04 <10 CFUs/g

Enterobacteriaceae No

2 SANS 21528-

2:2005 <10 CFUs/g

Yeast and Mould Yes 5 SANS 7954 <10 CFUs/g

Lactic acid bacteria Yes 4 SANS 15214:1998 <10 CFUs/g

Pseudomonas aeruginosa No

3

Method 2.5.2: 2003 Campden &

Chorleywood <10 CFUs/g

Sporeformers: Aerobic No 3 MFLP- 44 <10 CFUs/g

Sporeformers: Anaerobic No 3 MFLP- 44 <10 CFUs/g

Clostridium spores No

6 SANS 5761 (SABS

761) Detection/g

Thermophilic count No

3 CCFRA Method

1.1.1:2003 <10 CFUs/g

Gram negative ID No n/a Visual/Biochemical Microscope n/a n/a

pH No 2 pH meter

Pathogens n/a Minimum

25g

E. coli (confirmed) Yes

2 BIORAD 07/8 -

12/04 <10 CFUs/g

Staphylococcus aureus (confirmed) Yes 2

SANS 6888 - PART 2

<10 CFUs/g

Listeria monocytogenes (detection) Yes

3 BIORAD 07/04 -

09/98 n/a

Listeria monocytogenes (count) Yes

3 BIORAD 07/04 -

09/98 n/a

E. coli 0157 (detection) Yes

2 Merck - Single Path

Method

Bacillus cereus (presumptive count) Yes 2 SANS 7932

Clostridium perfringens (presumptive count)

Yes 2 SANS 7937

<10 CFUs/g

Vibrio species (detection) No 4 SANS method 6196

Salmonella (detection) Yes 4 SANS 6579:2003 n/a

Confirmations n/a n/a

Bacillus cereus Yes 3 SANS 7932

Clostridium spores No

5 SANS 5761 (SABS

761)

Clostridium perfringens Yes 3 SANS 7937

Listeria monocytogenes (confirmation)

Yes 2

BIORAD 07/04 - 09/98

Pseudomonas aeruginosa No

3


Chorleywood

Salmonella Yes 3 SANS 6579:2003

Vibrio spp. No 3 SANS method 6196

Vibrio cholera No 3 SANS 6315:2003

E. coli (USP only) No 3 USP- 33:62

Staphylococcus aureus (USP only) No 1 USP- 33:62

Pharmaceutical

TVC (Total Aerobic Microbial Count) No 3 USP - 33:61 <10 CFUs/g

Yeast and Mould (Total Combined Yeast & Mould Count)

No 5 USP - 33:61

<10 CFUs/g

Pseudomonas aeruginosa (detection)

No 3 USP - 33:62 Detection/g

E. coli (detection) No 4 USP- 33:62 Detection/g

Salmonella (detection) No 4 USP- 33:62 Detection/g

Staphylococcus aureus (detection) No 3 USP- 33:62 Detection/g

14 day sterility No

15 USP-33:71 Growth/No growth/ml

Pyrogen test No 2 USP- 33:85 Detection/LAL

Preservative challenge testing (5 organisms)

No 40 USP-33:51

<10 CFUs/g

Preservative challenge testing (7 organisms)

No 40 WWSP 008

<10 CFUs/g

TPC (Water) R2A No

6 BP 2010:IV APP XVI

B <1 CFU/volume

filtered

Airplates

TVC No 3 1 plate n/a

E. coli No 2 1 plate n/a

Yeast and Mould No 5 1 plate n/a

Coliforms No 2 1 plate n/a

Water

TVC (water) Yes 3 SANS 5221 <1 CFU/ml 50ml

Coliforms (water) Yes

2 SANS 5221 <1 CFU/volume

filtered 250ml

E. coli (water) Yes


filtered 250ml

Faecal Coliforms (water) Yes


filtered 250ml

Yeast and Mould (water) No


filtered 50ml

Pseudomonas aeruginosa (water) No

3


Chorleywood <1 CFU/volume

filtered 250ml

Vibrio cholera (water) No

4 SANS 6315:2003 Detection/volum

e filtered 500ml

Swabs

Sterile swabs n/a per swab

TPC (Total Plate Count) Yes

4 AOAC Official

Method 996.23 <10 CFUs/area

swabbed 1 swab

Coliforms Yes

2 BIORAD 07/8 -

12/04 <10 CFUs/area

swabbed 1 swab

E. coli Yes

2 BIORAD 07/8 -

12/04 <10 CFUs/area

swabbed 1 swab

Yeast and Mould Yes

5 SANS 7954 <10 CFUs/area

swabbed 1 swab

Lactic acid bacteria Yes

4 SANS 15214:1998 <10 CFUs/area

swabbed 1 swab

Pseudomonas aeruginosa Yes

3


Chorleywood


1 swab

Clostridium perfringens Yes


swabbed 1 swab

Staphylococcus aureus Yes

2 SANS 6888 - PART

2 <10 CFUs/area

swabbed 1 swab

Bacillus cereus Yes


swabbed 1 swab

Listeria monocytogenes (count) Yes

2 BIORAD 07/04 -

09/98 <10 CFUs/area

swabbed 1-3 swabs

Listeria monocytogenes (detection) Yes

3 BIORAD 07/04 -

09/98 <10 CFUs/area

swabbed 1-3 swabs

Salmonella (detection) Yes

4 SANS 6579:2003 <10 CFUs/area

swabbed 1-3 swabs

E. coli 0157 (detection) Yes

2 Merck -Single Path <10 CFUs/area

swabbed 1-3 swabs

Plates (Media) n/a n/a n/a n/a n/a Per plate

PCA plate

ECA plate

RBA plate

BPA plate

XLD plate

BGAM plate

TSA (RODAC) plate

SCA (RODAC)

MRSA plate

Buffered peptone water

introduction - microchem · introduction: microchem lab services (pty) ltd is an independent and...

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