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RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures

Introducing the SCIEX Triple Quad™ and QTRAP® 6500+ LC-MS/MS System with optional SelexION®+ Technology

SCIEX, Concord, Ontario (Canada)

2 © 2015 AB Sciex RUO-MKT-11-2491 For Research Use Only. Not for use in diagnostic procedures

Our Best Triple Quad Just Got Better – the Most Sensitive and Fastest SCIEX Triple Quad™ System

The New SCIEX 6500+ Series with IonDrive™ Technology

Improved IonDrive™ detection system

• Detect analytes across a wide scope of

chemistries, low mass or high mass, positive or

negative polarity

• Quantify with lowest limits of quantitation and up

to 6 orders of linear dynamic range

• Ultra fast polarity switching with Scheduled

MRM™ Pro algorithm for multi-target

quantitation

Elevated SelexION® technology

• Jet Injection technology with improved

transmission and no effect on resolution

Audible noise reduction

Industry proven performance of IonDrive™

Turbo V ion source and QJet® ion guide

Results in less time – fast, high-throughput,

simplified data processing tools

Service and support resources for success

New

New

New


Hardware Overview


Improved IonDrive™ Detection System

• New detector ‒ Larger detection area

• New power supply ‒ High energy dynode at 15 kV

improves sensitivity

‒ Enables faster 5 msec polarity switching

• “Floated” design ‒ Ions in negative polarity directed straight into the detector

‒ This benefits low mass ions which are detected much more efficiently than

in the 6500 system

Contributes to significant signal gains in negative mode Q3 fragment

ion <100 Da

S/N (LOQ) improvements are compound, matrix and LC dependent

Improved data quality in screening applications with ultra-fast 5 msec

polarity switching


Sensitivity of 6500+ System in Positive Polarity

0.1 pg/mL Testosterone (with SelexION®+ Technology, 50 µL Injection)


Linearity of 6500+ System in Positive Polarity

0.1 to 100 pg/mL Testosterone (with SelexION®+ Technology)

0.1 to 5 pg/mL

Accuracies: 92 to 109%

%CV: 3.8% at 0.1 pg/mL


Linearity of 6500+ System in Positive Polarity

1 to 100,000 pg/mL Flurazepam (with SelexION®+ Technology)

1 to 1000 pg/mL

5 orders

r > 0.9999


Sensitivity of 6500+ System in Negative Polarity

1 ng/L (1 part-per-trillion) PFOA and PFOS (Linearity 0.5 to 5000 ng/L)

Quantifier (blue)

Qualifier 1 (purple)

Qualifier 2 (orange)

Quantifier (blue)

Qualifier 1 (purple)

PFOA

PFOS

499/80 499/99

413/369 413/219 413/169


100 ppt PFBS

QTRAP® 5500 LC-MS/MS system


QTRAP® 6500+ LC-MS/MS system

MRM 298.9 / 80.0

~ 2x S/N improvement

MRM 298.9 / 99.0

~ 2.5x S/N improvement



0.1 ng/mL (100 ppt) Monofluoroacetate (Linearity 0.05 – 1000 ng/mL)

77/57 (blue)

77/33 (purple)

Internal standard

81/60 (blue)

81/35 (purple)

Accuracies: 96.1 to 110.9%

%CV: 4.99% at 0.1 pg/mL (n = 10)


Ultra Fast Polarity Switching of 6500+ System

Multi-Pesticide Screening for ~300 Analytes with 5 msec Switching

552 MRM transitions

Positive ESI

58 MRM transitions

Negative ESI

Flufenacet CV = 4.37%

Methoxyfenozide CV = 1.52%


Multi-Pesticide Screening with 5 msec Polarity Switching

Pesticide Standard and Sample Extracts (Positive Polarity)

1 ng/mL

Tomato (10x)

Banana (10x)

Lemon (10x)

Cucumber (10x)

Strawberry (10x)

Arugula (20x)


Multi-Pesticide Screening with 5 msec Polarity Switching

Pesticide Standard and Sample Extracts (Negative Polarity)

1 ng/mL

Tomato (10x)

Banana (10x)

Lemon (10x)

Cucumber (10x)

Strawberry (10x)

Arugula (10x)


Software Overview


Quantitation and Ion Ratio in MultiQuant™ Software

QuEChERS Extract of Cucumber (dSPE with PSA) and 10 x Dilution

Std. 1 Lemon Tomato Cucumber Strawberry Arugula

Propamocarb


QTRAP® Library Searching in MasterView™ Software

QuEChERS Extract of Cucumber (dSPE with PSA) and 10 x Dilution

Sample

MS/MS FIT = 100%

Standard



QuEChERS Extract of Strawberry (dSPE with PSA) and 10 x Dilution


Dimethoate


QTRAP® Library Searching in MasterView™ Software


Sample

MS/MS FIT = 100%

Standard





Fludioxonil (-)


SelexION®+ Technology Differential Mobility Spectrometry (DMS)


DMS Electrode 1

DMS Electrode 2

DMS Electrode 1

DMS Electrode 2

Lens

Lens

SelexION®+ Ion Mobility Device with Jet Injector Technology

• New DMS cell designed to improve ion

transmission by ~2x without loss in

selectivity or resolution

• Addition of lens increases ion velocities

into cell

• Reduces transit times through

detrimental fringing fields

• Only available on 6500+ platform

Continually driving selectivity improvements

SelexION cell

SelexION+ cell

DMS Electrode 1

DMS Electrode 2

DMS Electrode 1

DMS Electrode 2

Lens

Lens


6500+ DMS removed

Detection of Triazole with and without DMS

6500+ with DMS

Standard

0.5 ng/L

Spiked and extracted fruit

(10 µg/kg)

S/N ~ 390

S/N ~ 60

IS = 3000 V; TEM = 550ºC

N

NHN


Accuracy 97-103%

%CV < 2%

Linearity and Repeatability (0.01 mg/kg – 50 mg/kg; n = 3)


Added Selectivity to Simplify Sample Extraction

Example: 1 µg/kg Imidacloprid and Metabolites in Bee Pollen Analyzed

using QTRAP® 6500+ System with SelexION®+ Technology

Pollen extract 4x dilution, average sensitivity gain over 6500 with DMS = 3x

Imidacloprid Imidacloprid-IS

5-OH

Olefin


Detection of Somatostatin with and without SelexION+

6500+ DMS removed 6500+ with DMS

Somatostatin, a cyclic peptide with poor MS/MS fragmentation is acquired using MIM (819.7/

819.7) assay in rat plasma showing high background noise limiting the S/N ratio and LOQ

Highly reduced background noise enabling desirable LOQ of 100 pg/mL using new SelexION+ jet

injector on 6500+ System (S/N ratio = 12, accuracy = 101% and %CV < 10% )

100 pg/mL


Industry Proven Robustness and Ruggedness

Alprazolam %CV = 3.5%

Buprenorphine %CV = 7.9%

Clenbuterol %CV = 2.9%

Methamphetamine %CV = 2.8%

Repeatability of 4000 injections (crashed human plasma (1:3) diluted 1:2)

SelexION®+, IonDrive™ Turbo V Ion Source and QJet® Ion Guide


Industry Proven Robustness and Ruggedness

Alprazolam

Buprenorphine

Clenbuterol

Methamphetamine

Repeatability of 4000 injections (crashed human plasma (1:3) diluted 1:2)

1. Injection 1000. Injection 2000. Injection 3000. Injection 4000. Injection


Audible Noise Reduction

• Active (source exhaust modifications) ‒ Source exhaust flow minimized to match gas load

‒ Current shipping design assumes maximum

incoming source gas load at all times

• Passive (quieter tubing) ‒ Eliminate “whistling” noise

• Quieter system operation than 6500 ‒ Noticeable under most source conditions

‒ No impact to sensitivity

Decreased audible noise for a more comfortable work

environment


The New SCIEX 6500+ Series and SelexION+ technology

Industry proven performance: IonDrive™ Turbo V ion source

and QJet® ion guide

• Significant sensitivity boost in low mass

negative mode with “new HED+ detector

technology”

• Enhanced SelexION+ performance with

“new jet injector DMS cell” without

compromising resolution

• Fast polarity switching speed (5 msec)

• Audible noise reduction


Trademarks/Licensing

For Research Use Only. Not for use in diagnostic procedures.

© 2015 AB Sciex. The trademarks mentioned herein are

the property of AB Sciex Pte. Ltd. or their respective owners.

AB SCIEX™ is being used under license.

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