international journal of livestock research issn 2277-1964 ... · international journal of...

International Journal of Livestock Research eISSN : 2277-1964 NAAS Score -5.36 Vol 7 (1) Jan’17

[email protected] DOI 10.5455/ijlr.20161218125703

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Original Research

Effects of Treated Cocoa (Theobroma cacao) Bean Shell Based Diets on Serum

Biochemistry and Haematological Indices of Laying Hens

Olumide, M.D.*1

, Hamzat, R.A.2, Bamijoko, O.J.

3 and Akinsoyinu A.O

1

1*Department of Animal Science, Babcock University, Ilishan Remo, Ogun State, NIGERIA

2Department of Animal Science, Federal University, Dutsin-Ma, Katsina State, NIGERIA

3Federal College of Animal Production and Health, Moor Plantation, Ibadan, Oyo State, NIGERIA

*Corresponding author: [email protected]

Rec. Date: Sep 01, 2016 05:51

Accept Date: Dec 18, 2016 00:57

Published Online: January 08, 2017

DOI 10.5455/ijlr.20161218125703

Abstract

Cocoa Bean Shell (CBS) is a waste product from cocoa processing industries, with attendant

disposal problem. The level to which CBS can be utilized by layers was evaluated. The Raw CBS,

(RCBS), Enzyme Rovabio Treated CBS (ECBS) and CBS Fermented by solid state (FCBS) were

evaluated for their contents of Crude Protein (CP), Crude Fiber (CF), Ether Extract (EE),

Metabolisable Energy (ME) and Theobromine. Total of 140 laying hens at 6-week in lay was used in

this experiment with 20 birds per treatment, replicated twice. The experimental design is completely

randomized design. The experiment lasted for eight weeks. Hen Day Production (HDP), internal

and external egg quality characteristics were monitored. Data were analyzed using ANOVA. This

study investigated the effect of treated cocoa bean shell based-diets on serum biochemistry and

hematological parameters of layers. The variation observed in the total protein was significant

while the variations observed for serum biochemical indices like albumen, globulin and creatinine

were not significantly different among the dietary treatment. Fermentation and addition of enzymes

reduced theobromine contents to 0.72 and 0.81 respectively while the highest value was recorded

for RCBS (0.85). Hematological indices were not significantly different, however, highest serum

total protein values (5.16g/dl) was observed in birds fed control diet. Enzyme-treated cocoa bean

shell effectively replaced up to 10% maize in layers’ diets.

Key words: Cocoa Bean Shell, Laying Birds, Hematology, Serum Biochemistry

How to cite: Olumide, M. D., Hamzat, R. A., Bamijoko, O. J., & Akinsoyinu, A. O. (2017). Effects of Treated Cocoa (Theobroma cacao) Bean Shell Based Diets on Serum Biochemistry and Haematological Indices of Laying Hens. International Journal of Livestock Research, 7(1), 8–20. doi:10.5455/ijlr.20161218125703 Introduction

A minimum of 30g of animal and 40g of plant protein is recommended (FAO, 1994) and up till now,

Nigeria (and most other developing countries) still battle with the problems of inadequate protein intake

from animal sources. Campaign has been intensified in recent times, in countries beset with the problem

http://dx.doi.org/10.5455/ijlr.20161218125703



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of deficiency in dietary animal protein intake by encouraging micro–livestock production such as rearing

of snails, porcupine, and cane rats and so on, with a view to further bridge the gap of protein deficiency. It

is obvious that the production of these micro sources of animal protein cannot achieve much in solving

the deficiency problem as a result of their size, slow growth rate and reproducibility. The turnover of

protein derivable from micro livestock production is small compared with poultry, pig and ruminants. To

meet this target, animals with short generation interval, high fecundity and prolificacy are the likely

channels for providing the expected animal protein. Such animals include poultry, fish and swine and on

the long run cattle, sheep and goats. Among the livestock enumerated, the major antidote to this protein

deficiency is the development of poultry, which include table egg production. Their ability to utilize some

non-conventional feedstuff and agro-industrial by-products of no dietary value to man. Cocoa bean shell

(CBS) is a waste product from the cocoa industries. It is the thin husk surrounding the cocoa bean. These

CBS is high in nutritive potential but it is of limited use in animal feeds because of its theobromine

content (Olumide et al., 2015). Theobromine belongs to the same naturally occurring methylated xanthine

group as caffeine (Olubamiwa and Hamzat, 2005). In Nigeria, large quantities of cocoa by-products

(including discarded cocoa bean shell and husk) are produced annually by farmers and associated

processing Industries. Cocoa bean shell (CBS), cocoa pod husk (CPH) and cocoa bean cake (CBC) could

prove as animal feed ingredients. The purpose of investigating blood composition is to have a way of

distinguishing normal states from states of stress. The stress factors could be nutritional, environmental

or physical. Serum or plasma chemical values are useful in the assessment of the nutritional and health

status of animals. Dietary components have measurable effects on blood components and such blood

constituents are widely used in nutritional evaluation and survey of animals (Church et al., 1984;

Veulterinora, 1991; Olorode et al., 1996). Blood variables most consistently affected by dietary

influences include red blood cell counts, packed cell volume, plasma protein and glucose (Aletor and

Egberongbe, 1992). Serum level detection tells the existence of liver cells damage (Aniket, 2005), for

example if liver fails to deaaminate amino acids, nitrogenous wastes may accumulate in the body causing

harm. Babatunde and Pond, (1987) have established that packed cell volume and hemoglobin were

directly related to the nutritional balance of the diet fed to the animal and attributed decrease in total

protein to inhibition of protein utilization. Hematological parameters usually studied include erythrocytes

(RBC), packed cell volume (PCV, hematocrit), hemoglobin (Hb), Mean Corpuscular Hemoglobin

(MCH), Mean Corpuscular, Hemoglobin Concentration (MCHC), leucocytes total count (WBC)

thrombocytes (platelets) etc. (Jaini 1986). These parameters measure the condition of health of the animal

under study. Serum biochemical metabolites include electrolytes, blood proteins, plasma lipids and blood

glucose. Based on the previous work of Olumide et al., 2015, which concluded that broilers can

effectively consume up to 10% inclusion of RCBS in their diets without compromising health status. This



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study investigated the effect of treated cocoa bean shell based-diets on serum biochemistry and

hematological parameters of laying birds.

Materials and Methods

Experimental Site

This experiment was carried out at Kolmart Farm, Poultry Section, Erunmu, Ibadan, Oyo - State,

Nigeria. The birds were housed in an open-sided building in a thoroughly cleaned, washed and

disinfected two tier cage system of 32 x 38 x 42 cm dimension. The birds were caged individually.

Experimental Birds and Layout of the Experiment

A total of 140 laying hens at six week in lay were used in this experiment with 20 birds per

treatment, replicated twice. The experiment lasted for eight weeks. The cocoa bean shell was

sourced from cocoa industry in Lagos, while other feed ingredients were purchased from a

commercial feed miller in Ibadan. Seven diets were formulated as shown in Table 1, Diet A

(Control diet) contained no test ingredient (CBS). Diets B and C contained raw cocoa bean shell

(RCBS) with a 5% and 10% maize replacement respectively. Diet D and E contained 5 and 10%

CBS with the inclusion of Rovabio enzyme (ECBS) while diet F and G also contain 5 and 10%

fermented cocoa bean shell (FCBS).

Table 1- Gross Composition (g/100gDM) of Cocoa Bean Shell-Based Layer Diets

Ingredient A (0%) B (5%) C (10%) D (5%) E (10%) F (5%) G (10%)

Maize 55.54 52.76 49.98 52.76 49.98 52.76 49.98

RCBS - 2.78 5.55 - - - -

ECBS - - - 2.78 5.55 - -

FCBS - - - - - 2.78 5.55

SBM 12.13 12.13 12.13 12.13 12.13 12.13 12.13

GNC 8.00 8.00 8.00 8.00 8.00 8.00 8.00

Wheat offal 12.11 12.11 12.11 12.11 12.11 12.11 12.11

Bone Meal 2.55 2.55 2.55 2.55 2.55 2.55 2.55

Oyster Shell 8.67 8.67 8.67 8.67 8.67 8.67 8.67

Lysine 0.25 0.25 0.25 0.25 0.25 0.25 0.25

Methionine 0.20 0.20 0.20 0.20 0.20 0.20 0.20

Salt 0.30 0.30 0.30 0.30 0.30 0.30 0.30

Premix 0.25 0.25 0.25 0.25 0.25 0.25 0.25

Rovabio - - - 0.05 0.05 - -

Total 100.00 100.00 100.00 100.05 100.05 100.00 100.00

Calculated Composition Metabolisable

Energy 2631.06 2602.82 2574.57 2602.82 2574.57 2602.38 2585.09

Crude Protein % 16.05 16.17 16.34 16.17 16.34 16.20 16.40

Crude Fiber % 4.34 4.72 5.09 472 5.09 4.55 4.75

*RCBS-Raw Cocoa Bean Shell, *ECBS-Enzyme-treated Cocoa Bean Shell, *FCBS-Fermented Cocoa Bean Shell

**Composition Vitamin/Mineral Mix 1 kg (layers): Vitamin A 1000000IU, Biotin 40g, Vitamin B12 10mg.Folic acid

500mg, Manganese 4800MG, Zinc 58mg,Iron 5800mg, Selenium 120mg, Iodine 60mg, Cobalt 300mg. Composition

of methionine 20,000mg, Butylated hydroxytolerance BHT 50,000mg.



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Management of Experimental Birds

The experimental birds were raised until point of lay on commercial diet and to six weeks in-lay. The

birds were given adequate medication and vaccination before the commencement of the experiment. The

birds were weighed individually at the beginning of the experiment before they were placed on

experimental diets. Feed and water were supplied adequately and other daily routine managements were

provided. The performance records of daily feed intake, egg production and feed conversion ratio were

monitored.

Hematology and Serum Biochemistry

At the end week 8, 2 birds per replicate and a total of 4 birds per treatment were randomly selected and

bled by the jugular vein using needle and syringe. The blood was carefully labeled for hematological and

serum biochemistry analysis. The blood samples for hematological parameters were collected into bottles

pretreated with EDTA, an anti-coagulant. Blood samples for biochemical indices were collected into

another sample bottle containing no anti-coagulant. The samples for serum biochemistry were kept in the

refrigerator. The samples were spurned in the centrifuge at 3,000 rpm and the clearer portion decanted

into small sample tubes stored in a freezer to assay for serum biochemical indices. The hematological

indices monitored include Red Blood Cell Count (RBC), White Blood Cell Count (WBC), Packed Cell

Volume (PCV) and Hemoglobin Concentration (Hb). Serum biochemical indices investigated were total

protein, albumin, creatinine, globulin, albumin, globulin, cholesterol and glucose.

Analysis

Proximate analysis of the feed was carried out (AOAC, (1990). The anti – nutritional factor, theobromine,

in the diet was also determined (AOAC, (1990).

Statistical Analysis

Data collected were analyzed using analysis of variance (ANOVA) and significant means where

significant separated using Duncan Multiple Range Test (Gomez and Gomez, 1985). Statistical Analysis

Software (SAS, 1999) computer package was used.

Results

The gross composition of the layer diets is presented in Table 1. Metabolisable energy decreased from

2609.96 kcal ME/kg in diet A (control diet) to 2566.39 kcal ME/kg in diet G (10% FCBS). Table 2 shows

the determined analysis of experimental diets. Crude protein ranged between 17.00–17.40% (diet E).

Crude fiber increased from 4.10% (diet A) to 4.8% (diet G). Ether extract values decreased from 3.49%

(diet A) to 3.39% (diet G). The values obtained for ash ranged from 5.16 (diet G) – 10.00 (diet C).



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Table 2 - Proximate Composition of Cocoa Bean Shell-Based Diet

Parameters A

0%

B

5%

C

10%

D

5%

E

10%

F

5%

G

10%

Crude Protein (%) 17.00 17.15 17.30 17.20 17.40 17.18 17.38

Crude Fiber (%) 4.10 4.46 4.82 4.44 4.76 4.51 4.80

Ether Extract (%) 3.49 3.46 3.40 3.46 3.44 3.44 3.39

Ash (%) 5.48 8.05 10.00 4.89 5.77 5.32 5.16

Nitrogen Free Extract

(%)

69.93 68.88 64.48 70.01 68.63 69.55 69.27

Metabolisable Energy

Kcal/kg/DM

2620.10 2601.00 2573.83 2610.0 2590.03 2608.53 2589.32

Theobromine % 0.00 0.03 0.06 0.02 0.04 0.01 0.03

No of observation (2)

Performance Characteristics

Table 3 shows the result of performance characteristics. The feed intake ranges from 89.40 for treatment

G and 98.00 for treatment D. Treatment D had the highest percentage hen day production of 86.68, while

the lowest 78.17 was obtained from treatment G. Although, differences in HDP of birds on diet D and E

(ECBS) were not significant compared with control, the birds on diet D had the highest hen day

production followed by those on diets A and E. The egg weight of birds on the RCBS was lower than all

other treatments; this could be attributed to the presence of theobromine. Egg weight of birds on diet with

enzyme CBS diets D and E (5 and 10%) has higher egg weight compared with the control diet. The FCR

obtained in this study ranged from 2.43–2.65. The absence of mortality throughout the period of this

experiment further attested to the suitability of CBS as a substitute for maize in layers diet. This also

indicates that layer can tolerate CBS based diet than broilers.

Table 3 - Performance Characteristics of Layers Fed Variously Treated CBS Based Diet

Parameters A

0%

B

5%

C

10%

D

5%

E

10%

F

5%

G

10%

SEM

Feed Intake (g) 98.80a

95.38bc

93.96cd

98.00a

97.06ab

92.16d

89.40e

0.46

Hen Day Egg

Production %

85.56ab

83.01b 81.51c

86.68a

85.27ab

81.01c

78.17d

0.53

Egg Weight (g) 61.37bc

60.06c

60.49c

65.56a

64.61a

63.24b

62.26b

3.19

Feed Conversion

Ratio

2.44 2.47 2.55 2.43 2.48 2.53 2.65 0.05

Mortality (% ) Rate 0.00 0.00 0.00 0.00 0.00 0.00 0.00 -

a, b, c: Means along the same row with any identical superscripts are not significant (P > 0.05). No of Observation

(2)

Serum Biochemistry and Hematological Characteristics of Layers Fed CBS -Based Diets

The results of the serum biochemistry and hematological parameters of birds fed CBS based diets are

presented in Table 4. Variations observed in the total protein were significant, although as the level of



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CBS in the diet increased the total protein level in the blood also decreased. However, variations observed

for serum biochemical indices like, albumen globulin and creatinine were not significantly different

(P>0.05) among the dietary treatments. There were also significant differences in values obtained for

Albumin: Globulin ratio, glucose and cholesterol value. The highest serum albumin value of 3.15 g/dl

was obtained from birds on diet G while the least was obtained from diet E (2.16 g/dl). The globulin

varied from 1.71–2.76 g/dl. Diet G has the highest value of albumin-globulin of 2.67 and diet A had the

least value of 0.88. Variation observed in the glucose levels were significant (P < 0.05). Birds on diet C

(10%RCBS) had the highest glucose concentration (mg/dl) of 136.45 while birds on diet G had the least

109.10. Although, the glucose concentration increased as the level of substitution increases for all the

birds on the diets. The serum creatinine levels (mg/dl) were not significantly different, the value ranged

from 1.19–1.40. Although as the level of CBS increases the creatinine level in the serum increases. Total

cholesterol levels significantly decreased (P<0.05) as the level of substitution of CBS for maize increased.

The results of the hematology of layers fed variously treated CBS based diet indicted that all

hematological parameters (Red Blood Count, White Blood Count, Packed Cell Volume and Hemoglobin)

monitored were similar across the dietary treatments. The red blood cells (mm3

x 106) ranged between

2.45–2.93, while the white blood cells (mm3 x 10

3) varied from 19.94–20.72 without following a

particular trend among dietary treatments. The lowest packed cell volume of 21.25% was obtained from

birds fed the control diet A and the highest value of 26.00% observed in birds fed diet B. The other PCV

values were within these two values. The hemoglobin concentration (g/100ml) was highest (8.66) in

birds fed diet B (5% RCBS) and lowest (7.34) in birds fed diet F (5% FCBS).

Table 4 - Serum Biochemistry and Hematological Characteristics of Layers Fed CBS–Based Diets Treatments

Parameters A B C D E F G SEM

Total Protein (g/dl) 516a

5.09a

4.95ab

4.95ab

4.06b

4.64ab

5.14a

0.18

Albumin (g/dl) 2.40 3.37 2.87 2.33 2.16 2.43 3.15 0.30

Globulin (g/dl) 2.76 1.71 2.08 2.26 1.90 1.99 2.22 0.12

Albumin: Globulin Ratio 0.88e

2.15b

1.53c

1.12d

1.13d

1.21d

2.67a

0.31

Glucose(mg/dl) 119.94bc

117.05cd

136.45a

129.50ab

136.42a

107.90d

109.10d

2.10

Creatinine(mg/dl) 1.33 1.30 1.40 1.27 1.31 1.79 1.23 0.11

Cholesterol(mg/dl) 139.50a

123.41ab

115.80b

122.58ab

112.77b

127.80ab

127.80ab

3.44

Red Blood Cell (mm3 x 10

b) 2.87 2.64 2.93 2.72 2.27 2.67 2.45 0.26

White Blood Cells (mm3 x

103)

19.94 20.07 20.51 19.07 20.45 20.72 19.95 0.77

Packed Cell Volume (%) 21.25 26.00 25.25 22.25 22.00 23.50 23.00 2.03

Hemoglobin (g/100ml) 7.58 8.66 8.08 7.33 7.58 7.34 7.67 0.55

a,, b, c, d, e: Means along the same row with any identical superscripts are not significant (P>0.05).



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Discussion

Proximate Composition of Experimental Diets

Birds on the control diet recorded the highest calculated metabolisable energy, ME (kcal/kg) and this

decreased with increased contents of CBS in the diets. The analyzed proximate composition (g/100DM)

of the experimental diets revealed that the percentage crude protein increases as the level of inclusion of

various CBS increases in the diet. The crude fiber level in the diet also increases with increase in the level

of CBS in the diet while the ether extract values reduce accordingly. No particular trend was observed in

the ash value obtained. Nitrogen free extract did not follow any particular trend but the values obtained

were close for all the dietary treatments. The highest values for crude protein was obtained for diet E

(17.40%) while the least value was obtained for the control diet (17.00). The control diet (A) had the

lowest crude fiber value (4.10%) while the highest value was obtained with diet C (4.82%). The highest

ether extract value of 3.49% was obtained in the control diet. The metabolisable energy (kcal/kg) values

of all the diets met the levels recommended by Olomu, (1979) and Fetuga (1984). Although the dietary

crude protein levels in all the diets met the values recommended by National Agriculture Extension and

Research Liaison Services NAERLS, (1990) and Olomu, (1995). The crude fibre falls within 3–5%

recommended by NRC (1994) for layers. Bentil et al. (2015) also suggested that the nutritive value of

cocoa bean shells for use as feed for animals through solid state fermentation.

Performance Characteristics

Variations observed in the feed intake (FI), hen day egg production (HDEP) and Egg Weight were

significant except feed conversion ratio. The feed intake of the birds on the control diet was higher and

similar to those on diet D and E (5 and 10%ECBS). This is in line with the findings of Iyayi and

Okhankhuele, (2002) who reported that birds on enzyme supplemented diets performed significantly

better than those on other diets, in cassava leaf meal based diets supplemented with enzyme. However,

the average daily feed intakes of the birds on the RCBS were lower than those on the control diets A, D

and E (ECBS). This could be as a result of the anti-nutritional factor theobromine in the diet. As the

level of CBS in all the diet increased the feed intake declined. Several reports (Yeong et al., 1989; Odunsi

et al., 1999; Olubamiwa et al., 2002) depicted reduced feed intake by laying birds fed CBS and cocoa

bean cake based-diets due to the theobromine content of the diet. Reduced feed intake is believed to be

due to destruction of the intestinal lining and severe indigestion in the birds (Yeong et al., 1989;

Olubamiwa et al., 2000). Although, differences in HDP of birds on diet D and E (ECBS) were not

significant compared with control, the birds on diet D had the highest hen day production followed by

those on diets A and E. This apparent increase in egg production could be due to the enzyme added to

diets D and E. Also, Olukosi and Adeola, (2007) reported that supplementation of wheat based diet with



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combination of xylanase and phytate improved growth performance of broiler chickens. However, the

HDP and egg weight obtained in this study were higher than those reported elsewhere (Keshavarz and

Nakajima, (1995). This could be due to the fact that the protein requirement of these birds was met at

lower level of 10% CBS as noted (Junqueira et al., 2006. The slight decrease in metabolisable energy/

(kcal/kg) and ether extract, and increase in crude fibre with increased levels of substitution could be

responsible for slight numerical decreases in HDP obtained with increased levels of substitution of CBS

for maize. This is in line with the report (Reid et al., 1984) which observed an increase in egg production

of single comb white leghorns pullets fed 16% dietary protein. As metabolisable energy increased from

2.42 to 2.64 and from 2.68 to 3.08 kcal ME/g diet there was a 2.25% increase in egg production for every

0.22 kcal ME/g increase in the diet. Egg production was however not affected by dietary energy level

(Harms et al., 2000). The values of ADFI (87.00–98.80g/bird/day) and HDEP (78.17–86.68%) obtained

in this study were higher than the corresponding values with spent sorghum grain and whole cassava meal

(Aderemi et al., 2006). The HDEP of 77– 78% obtained at the peak production (9–10 weeks) of Nera

(Majaro, 1999) were lesser than those obtained in this study. However, while higher ADFI was obtained

for birds fed kola pod-based meal by Olubamiwa et al., (2000), the percentage HDEP (64.5–71.3)

recorded by these authors were far below the values obtained in the present study. The egg weight of

birds on the RCBS was lower than all other treatments; this could be attributed to the presence of

theobromine. Previous studies have shown that theobromine reduces feed intake which leads to lower

laying rate and egg size (Hatagalung and Chang, 1978, Odunsi et al., 1999, Olubamiwa et al., 2002). Egg

weight of birds on diet with enzyme CBS diets D and E (5 and 10%) has higher egg weight compared

with the control diet. This shows that addition of enzyme Rovabio effectively reduce the anti-nutritional

factor in cocoa bean shell. According to Viveros et al., (2002) effect of anti-nutrients is markedly reduced

when diets are supplemented with enzymes by interfering with the antinutrients-protein complexes and

thereby releasing these proteins for digestion. This improved performance of birds on enzyme-

supplemented diets is brought about by improved absorption of fats and fatty acids as well as fat-soluble

micronutrients contained in the diet (Danicke et al., 1999).

The FCR obtained in this study 2.43–2.65 was lower when compared with that recorded by Kwari et al

(1999) 4.14–4.92 but fall with the same range with that of Fasuyi et al (2005) 2.38–2.76. The absence of

mortality throughout the period of this experiment further attested to the suitability of CBS as a substitute

for maize in layers diet. This also indicates that layer can tolerate CBS based diet than broilers. When all

the performance of the laying birds fed 5% of the various forms of CBS based diet was compared, there

was no significant difference in value of ADFI, HDP, egg with and FCR but when compared at 10%,

significant difference (P > 0.05) was observed in the ADFI, HDP and FCR.



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Serum Biochemistry and Hematological Characteristics of Layers Fed CBS -based Diets

The values obtained for the total protein were significantly different from each other. As the level of CBS

in the diet increases, the serum protein level reduces for the RCBS and ECBS except for that of FCBS

that increases. This significant variation observed in the values of total protein, albumin: globulin was an

indication of bad quality protein but contrarily the values obtained for the albumin and Globulin shows

that there are no significant variations observed, this was an indication of the good quality protein and

other nutrients fed to these birds as well as adequate metabolism of these nutrients to meet the

requirement of these birds. Olorode et al., 1996 confirmed that the significant (P < 0.05) reduction in

total protein of birds fed 10 and 15% shea butter cake was an indication of inferior protein quality and/or

nutrition. The values of total protein obtained for all the diets were lesser than that recommended by

Mitruka and Rawnsley (1977) except for the control. This could be as a result of the anti-nutritional

factor theobromine in the diets. While the values obtained for the albumin, glucose, creatinine fell within

the ranges 2.10–3.45 g/dl, 152–182 mg/dl and 0.90 – 1.85 mg/dl respectively for normal chickens by

Mitruka and Rawsley (1977). It was observed that as the level of inclusion of variously treated CBS in the

diets increases the total albumin level reduces except for the FCBS which increases as the level of

replacement increases for maize. This could be as a result of the theobromine content. The significant

increases obtained in the serum glucose level with increasing levels of variously treated CBS in the diets

is desirable especially when the highest content of 136.45mg/dl obtained from treatment C was far below

152mg/dl recommended by Mitruka and Rawsley (1977) for normal chickens. The author has

recommended a range of 152–182 mg/dl for normal chicken. The differences in the result of this study

compared to the recommendation of these authors could have been caused by differences in factors like

diets among others. Although, no significant difference was observed in the values of creatinine obtained

in this study. It was observed that as the level of variously treated CBS in the diets increased the level of

creatinine also increased although the highest value was obtained from the serum of the birds fed RCBS

based diet (1.40 mg/dl). This may point to slight tissue damage in birds fed 10% RCBS based diets. The

highest serum cholesterol was obtained from the control and the values significantly (P<0.05) decreased

with increasing levels of substitution of RCBS and ECBS for maize in the diets, except for the FCBS that

was slightly reduced. The consistent reduction in the serum cholesterol obtained with the increasing

substitution of variously treated CBS for maize in the diets could be explained by the increasing contents

of anti-nutritional factor and fibers that was associated with the increased levels of substitution. Anti-

nutritional factor like saponins have been found to assay a physiological effect on lowering the level of

plasma cholesterol concentration in experimental animals (Potter et al., 1979; Oakenful, 1981). Results

of past research works have suggested that lowering effect of fibre on liver lipid in poultry (Patel et al.,

1979; Longe, 1984; Scheeman, 1990) Dietary fiber has the ability to bind nuclear components like bile



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acids and phospholipids, thus slowing the absorption of lipid from the small intestine and so lowering the

plasma cholesterol and liver fat accumulation (Schneeman, 1990). Also, in the light of this, Donnica

(2000) and Lazaro et al (2003) recommended the consumption of soluble fibers’ to lower blood

cholesterol. The globulin values obtained in this study, although significantly different from each other,

but they were higher than the recommended values e.g. 0.45–0.96 g/dl by Mitruka and Rawsley, (1977).

Although no particular trend was followed with the increasing level of substitution of RCBS, ECBS and

FCBS. Albumin: Globulin obtained was lesser than the values recommended by the previous authors for

albumin globulin ratio (2.10–3.45) except for those obtained by diet B and G. The non-significant

differences in all the hematological parameters investigated in the birds fed the control and variously

treated CBS–based diets could explain absence of deleterious effect that could be linked with the diets fed

to these birds. No particular trend was observed in the hematological parameters with the increase in CBS

based diets. All the parameters falls with those recommended by Mitruka and Rawsley, (1977) 1.58-3.82

10c1

mm3, 7.4–12.2 g/dl, 9.2–28.610

6mm

3 for RBC ,Hb and WBC while some of the PCV values falls

below the recommend value of the above authors (24.9–40.7) for normal chickens. Oladele et al., (2001)

attributed the lower values of packed cell volume, hemoglobin and total protein of extensively managed

indigenous diet recorded during dry season to low level of nutrition especially protein deficiency, due to

scarcity of vegetables and farm left over’s to forage on. Ikhimioya et al., (2000) have suggested that the

low erythrocytes’ parameters (PCV and RBC) an anemic condition recorded in scavenging Nigerian

indigenous chicken was caused by poor nutrition resulting from their ability to ingest enough of highly

nutritious feeds while scavenging for daily nutrient need. Serum and hematological indices such as album,

globulin, creatinine, red blood cell count, blood cell count, packed cell volume and hemoglobin were not

significantly (P >0.05) affected by dietary treatments. Creatinine and glucose levels in the blood increased

while cholesterol decreased significantly (P <0.05). The creatinine and cholesterol levels of the birds fed

variously treated CBS based diets were still within the value recommended for chickens. The increase in

values with increasing contents of various forms of CBS based diets was desirable since the maximum

content (136.45 mg/dl) at 10% RCBS was the closest to the minimum value in a range of 152-182 mg/dl

recommended for normal chicken by these authors. The increased serum glucose content with increasing

dietary forms of CBS-based diets was desirable, since the maximum content (136.45mg/dl) at 10% RCBS

was the closest to the minimum value in a range of 152-182mg/dl recommended for normal chicken.

Conclusion

Results of various investigations carried out with CBS showed that it is a suitable alternative for maize in

the diets of layers. Its nutritional qualities could further be enhanced to allow for incorporation into the

diets of poultry at higher levels than adopted in the present study. It is therefore concluded that cocoa



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bean shell can be used to replace maize up to 10% in the diet of commercial layers without any

deleterious effects on the performance, hematology and egg quality parameters.

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