interactions between stimulus dynamics and network...
TRANSCRIPT
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Supplementary data
Interactions between Stimulus Dynamics and Network Activity in the Representation of Natural Auditory Scenes
Chandramouli Chandrasekaran1, 2, Hjalmar K. Turesson1,2, Charles H. Brown3, and Asif A. Ghazanfar1, 2, 4
1Neuroscience Institute Departments of 2Psychology and 4Ecology & Evolutionary Biology Princeton University Princeton NJ 08540, USA 3Department of Psychology University of South Alabama Mobile AL 36688, USA
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Supplementary figure legends
Supplementary figure 1: Periodic click trains have a multipeaked temporal structure — related to figure 1
A: An artificial synthesized click train with an approximate frequency of 6 Hz. X-axes depict time in seconds, Y-axes the amplitude in Hilbert units.
B: Fourier spectrum of the click train shown in A. Note the multiple peaks and the resemblance to the spectrum of the Savannah acoustic scene.
Supplementary figure 2: Spectrotemporal structure of natural acoustic scenes – related to figure 1
A: Modulation spectra of two other Savannah acoustic scenes as a function of both temporal and spectral frequency. X-axes depict temporal frequency in Hz; Y-axes depict spectral frequency in kHz. Color bar denotes the power in log units. B: Left, average spectral structure in the sound for the three different Savannah scenes. Red – Savannah 01, green Savannah 02, blue – Savannah 03. X-axes depict spectral frequency in kHz; Y-axes depict power in log units. C: Temporal modulation spectra of the three Riverine acoustic scenes as a function of both temporal and spectral frequency. Figure conventions as in A. D: Left, average spectral structure in the sound for the three different Riverine forest scenes. Red – Riverine forest 01, green Riverine forest 02, blue – Riverine forest 03. X-axes depict spectral frequency in kHz; Y-axes depict power in log units. E: Power in the modulation spectrum as a function of temporal frequency for three different selected spectral frequencies marked as 1,2,3 in the modulation spectrum for the Riverine 03 acoustic scene (Figure C) — red – 5.5 kHz, green – 7 kHz, blue – 3.45 kHz. X-axes depict temporal frequency; Y-axes depict power in log units F: Global temporal structure computed by summing over all spectral frequencies for the three different Riverine scenes. X-axes depict temporal frequency in Hz; Y-axes depict log power. G: Modulation spectra of the three other Rainforest acoustic scenes as a function of both temporal and spectral frequency. Figure conventions as in A. H: Left, average spectral structure in the sound for the three different Rainforest forest scenes Red – Rainforest 01, green Rainforest forest 02, blue – Rainforest 03. X-axes depict spectral frequency in kHz; Y-axes depict power in log units.
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I: Power in the modulation spectrum as a function of temporal frequency for three different selected spectral frequencies marked as 1,2,3 in the modulation spectrum for the Rainforest 03 acoustic scene (G) — red – 5.1 kHz, green – 7.2 kHz, blue – 3.3 kHz. Figure conventions as in E J: Global temporal structure pooled by summing over all spectral frequencies for the three different rainforest scenes. Conventions as in F. Supplementary figure 3: Change in power of the LFP during natural scene stimulation – related to figure 2 A: Power spectrum of the LFP response from 3 cortical sites during the presentation of the Riverine forest acoustic scenes. X-axes depict frequency in Hz. Y-axes on the left depict the power in logarithmic units. Y-axes on the right denote the percent enhancement of power relative to baseline. B: Percent enhancement of the power in the LFP during Riverine acoustic scenes relative to the baseline. X-axes depict frequency in Hz. Y-axes depict the enhancement in percent. C: Percent enhancement of the power in the LFP during Rainforest acoustic scenes relative to the baseline. Conventions as in B. D: Negative logarithm to the base 10 of the p value obtained from t-tests comparing the power spectrum during the natural scene stimulation to the power spectrum during the baseline period. X-axes depict frequency in Hz. Y-axes depict the –log10 (p) in arbitrary units. The dotted line denotes the Bonferroni corrected p-value cutoff. Supplementary figure 4: phase coherence within a given cortical site is consistent across intact savannah exemplars – related to figure 3. Phase coherence between the LFP and the Savannah scene for all possible combinations of Savannah scenes and LFP. Rows denote different LFP in response to different Savannah scene exemplars and used as input to the phase coherence. Columns denote different Savannah scene envelopes. X-axes in all 9 plots depict frequency; Y-axes depict phase coherence. Error bars denote bootstrapped standard errors obtained by resampling the cross spectral phase angle distribution 1000 times. Supplementary figure 5: Phase coherence between spikes and LFPs to Riverine and Rainforest scenes – related to figure 5 A: Average phase coherence between the LFP and Riverine scene averaged over the population of cortical sites (gray lines) for intact (thick red lines) versus shuffled (thick black line). Thin gray traces represent individual sites for intact phase coherence measures. X-axes depict frequency in Hz; Y-axes on the left depict phase coherence for the single exemplars. Y-axes on the right depict the phase coherence for the average. B: Average rank ordered intact and shuffled phase coherence between Riverine scene and the LFP for specific frequency bands. X-axes depict cortical site number; Y-axes depict phase coherence.
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C: Average phase coherence between the LFP and Rainforest scene averaged over the population of cortical sites for intact (thick red lines) versus shuffled (thick black line). Thin gray traces represent individual sites for intact phase coherence measures. Figure conventions as in A. D: Average rank ordered intact and shuffled coherence between Rainforest scene and the LFP for specific frequency bands. Conventions as in B. E: Average phase coherence between the spiking activity and Riverine scene averaged over the population of cortical sites for intact (thick green lines) versus shuffled (thick black line). Conventions as in A. F: Average rank ordered intact and shuffled coherence between Riverine scene and the spiking activity for specific frequency bands. Conventions as in B. G: Average phase coherence between the spiking activity and Rainforest scene averaged over the population of cortical sites for intact (thick green lines) versus shuffled (thick black line). Thin gray traces represent individual sites for intact phase coherence measures. Figure conventions as in A. H: Average rank ordered intact and shuffled coherence between Rainforest scene and the spiking activtity for specific frequency bands. Conventions as in B. Supplementary Figure 6: Phase coherence between LFP and natural scenes as a function of both spectral and temporal modulation frequency – Related to figure 6. A: Percent of cortical sites that showed enhanced phase coherence between Riverine scenes and LFP as a function of both spectral and temporal frequency. X-axes depict temporal frequency in Hz; Y-axes depict spectral frequency in kHz. Colorbar denotes the percentage of sites. B: Percent of cortical sites that showed enhanced phase coherence between Rainforest scenes and LFP as a function of both spectral and temporal frequency. Conventions as in A. Supplementary figure 7: Spike field phase coherence during the processing of natural scenes – related to figure 8, 9. A: Left, plot of correlation coefficient between Riverine scene-LFP phase coherence and spike-field phase coherence for the population of cortical sites. Horizontal error bar denotes the standard error over the 3 intact pairs. Vertical error bars denote the standard error over correlations performed for the 3 intact pairs. X-axes depict Riverine scene-LFP phase coherence; Y-axes denotes the correlation coefficient. B: Percent enhancement of the spike field phase coherence relative to the baseline for the population of cortical sites during Riverine scene stimulation. X-axes depict frequency in Hz. Y-axes
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the enhancement in percent. Gray lines denote points at which the spike field phase coherence was significantly different from baseline (p < 0.05, corrected for multiple comparisons). C: Percent enhancement of the spike field phase coherence relative to the baseline for the population of cortical sites during the Rainforest scene stimulation. Figure conventions as in B.
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