insemination technique - smile you are vet -...
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Dr Hany lotfi
Insemination technique
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1- Transportation
Liquid semen
Frozen semen
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2- Detection of the estrus
a- Observer:
b- Using marked teaser (vasectomized male):
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c- Using mount heat detector:
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c- Pedometer
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3- Examination of the cow
The record of the cow must be examined for
- Date of last parturition
-Date of last insemination
- Examination after insemination
Clinical examination of the cow
- Freedom from pregnancy
- Estrus finding
- Healthiness of reproductive tract
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4- Best time for insemination
Beginning sings of estrus Optimum time for insemination
Early morning
Late on the day
Late in the same day
Next morning
Mid of the day Too late in the same day
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Heritability for some traits in dairy cattle 4- Selection of the male
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Predicted transmitting ability (PTA)
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5- Instrument required for insemination
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6- Retrieval of the frozen sample from the tank
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Thawing
Straw
Pellet
Ampoule
Water path at 37 oC for 30 sec
Water path at 35 oC for 60 sec
Emersion on 0.8ml of Sod. Citrate
2.9% at 37 oC
Ice water at 5 oC for 10 min
Warm water 20-38 for 1 min
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Inseminating gun
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Split sheathesNon-split
sheaths O ring Spiral Kombicolor
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Loading of the sample into insemination gun
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Instrument used for liquid semen and frozen semen as
pellet or ampoule
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Semen deposition into female genital tract
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Cervical Uterine body
1- No possibility of uterine damage 1- Possibility of uterine damage
2- Not interrupt the pregnancy 2-Interrupt the pregnancy
3- Utilize the antimicrobial agent
of cervical mucus
4- Non motile m.o. may not pass to
the uterus
3- Easily establish the infection
5- It is easier and quickly performed.
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Insemination of mare
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The old protocol include insemination of the mare every 24- 48 h
beginning on the third day of estrus and continuing until she goes
out of estrus
Insemination of the mare with one dose of semen per cycle. The
second include insemination of the mare with two doses before and
after ovulation.
To control the time of ovulation by application of as ovulatory agent
in conjunction with the examinations to reduce the number of
reproductive examinations.
Timing of Insemination
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