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Mini-Symposium on Initiatives in (Analytical) Sciences Linkage to Local Issue-Global Impacts-Sustainable World” y = 0.4658x + 6.9881 R² = 0.9889 0 20 40 60 80 0 20 40 60 80 100 120 Relative R intensity Ammonium-nitrogen, mg/L in downscaling cost effective chemical analysis Initiatives using moving drop and other novel platforms moving drop other novel platforms Chiang Mai University - Prof. Dr. Kate Grudpan, Dr. Sutasinee Apichai, Dr. Suphasinee Sateanchok, Dr. Khajorngai Thajee and Ms. Pheeraya Jaikang Rajabhat Universities - Lampang Rajabhat University; Mr. Narong Kotchabhakdi Buriram Rajabhat University; Ms. Chuleekant Sainate Chiang Rai Rajabhat University; Dr. Napaporn Wannaprom & Dr. Yaowalak Khanhuathon Rajamangala University - Rajamangala University of Technology Lanna Chiang Rai; Dr. Karuna Jainontee King Mongkut's University of Technology Thonburi ; Asst. Prof. Dr. Monapat Vongboot Stirring piece for coffee The solution of reagent and sample are dropped on the position A and B. The change of reaction is monitored by taking a photo or recording a video. The signals of color solution on a position D are translate by using the software. The signals are statistically evaluated. 1 2 3 4 Colorimetric assay Kinetic study Iron assay Copper assay Phosphate assay using 1,10-phenanthroline reagent using bicinchoninic acid method using molybdenum blue method Intensity of color Concentration of analyte In (rate) In concentration of analyte NH 3 NH 4 + OH - + PTFE thread sheet Natural indicator- immobilized paper Transparent sticker Natural indicator - immobilized paper preparation Adjust pH Dried butterfly pea flower Butterfly pea flower extract Natural indicator- immobilized paper Filter paper Relative R intensity NH 4 + -N, mg/L NH 4 + -N solution NaOH solution 2 1 The natural material sensor with downscaling pervaporation and gas diffusion for ammonium - nitrogen determination 10 20 40 60 80 100 (mg/L)

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Mini-Symposium on Initiatives in (Analytical) Sciences

Linkage to Local Issue-Global Impacts-Sustainable World”

y = 0.4658x + 6.9881R² = 0.9889

0

20

40

60

80

0 20 40 60 80 100 120

Rel

ativ

e R

inte

nsi

ty

Ammonium-nitrogen, mg/L

in downscaling cost effective chemical analysis Initiatives using moving drop and other novel platforms moving drop other novel platforms

Chiang Mai University - Prof. Dr. Kate Grudpan, Dr. Sutasinee Apichai, Dr. Suphasinee Sateanchok, Dr. Khajorngai Thajee and Ms. Pheeraya Jaikang

Rajabhat Universities - Lampang Rajabhat University; Mr. Narong Kotchabhakdi

Buriram Rajabhat University; Ms. Chuleekant SainateChiang Rai Rajabhat University; Dr. Napaporn Wannaprom & Dr. Yaowalak Khanhuathon

Rajamangala University - Rajamangala University of Technology Lanna Chiang Rai; Dr. Karuna Jainontee

King Mongkut's University of Technology Thonburi; Asst. Prof. Dr. Monapat Vongboot

Stirring piece for coffee

The solution of reagent and sample are dropped on the position A and B.

The change of reaction is monitored by taking a photo or recording a video.

The signals of color solution on a position D are translate by using the software.

The signals are statistically evaluated.

1 2 3 4

Colorimetric assay Kinetic study

Iron assay

Copper assay

Phosphate assay

using 1,10-phenanthroline reagent

using bicinchoninic acid method

using molybdenum blue method

Inte

nsi

ty o

f co

lor

Concentration of analyte

In (

rate

)

In concentration of analyte

NH3

NH4+ OH-+

PTFE thread sheet

Natural indicator-immobilized paper

Transparent sticker

Natural indicator-immobilized paper preparation

Adjust pH

Dried butterfly pea flower

Butterfly pea flower extract

Natural indicator-immobilized paper

Filter paper

Rel

ativ

e R

inte

nsi

ty

NH4+-N, mg/L

NH4+-N solution

NaOH solution2

1

The natural material sensor with downscaling pervaporation and gas diffusion for ammonium-nitrogen determination

10 20 40 60 80 100 (mg/L)