0 5000 10000 15000 20000 25000 30000

ORF1ab S 3aEM6 7a 7b 8

N

3a E M 6 7a7b 8 N 3‘ UTR

10

1 2 3 4 5 7 8 9 10 11 1 2

10

Length (nt)

Transcripts

RNA Fragments

SARS-CoV-2 positive control

Information and Data Sheet

Product Catalogue No. Reactions

SARS-CoV-2 positive control 20

SARS-CoV-2 positive control 100

cv-C020-000567

cv-C100-000567

20

100

98% Coverage of SARS-CoV-2 Transcripts

siTOOLs Biotech SARS-CoV-2 positive control contains RNA fragments (120 – 2000 nt) covering 98% of the SARS-CoV-2 transcriptome. It is applicable in RT-PCR or LAMP detection assays.

3‘ UTR

Scale (ng)

2

10

SARS-CoV-2 positive control has twenty RNA fragments that cover all major transcripts, enabling its versatile application as a quality control.

2000

ORF1ab #1, #2, #3, #4, #5, #7, #8, #9, #10, #11

1800-2000

S #1, #2

ORF3a 830

E 230

M 670

Length (nt)

ORF6 190

ORF7a 370

ORF8 370

N 1260

120ORF10

FragmentFragment Length (nt)

Contact Us!info@sitools.de | +49 (0) 89 12501 4800 | www.sitoolsbiotech.com | siTOOLsBiotech

Wide Detection Range with SARS-CoV-2 Positive ControlReal-time quantitative PCR analysis of SARS-CoV-2 positive control using primers against fragments ORF1ab #8, E and N showed good detection sensitivity across five log-dilutions.

N-Gene E-Gene

Cq

2 3 4 5 6 7 8 9 Log Starting Quantity R2=0,992

25

20

15

10

5

Cq

2 3 4 5 6 7 8 9 Log Starting Quantity R2=0,959

25

20

15

10

5

Cq

2 3 4 5 6 7 8 9 Log Starting Quantity R2=0,949

25

20

15

10

5

Fragment #8

DS-260520-v1

ORF1ab #8 (Rdrp)

E

Fragment Forward Primer (5’ -> 3’)*

Reverse Primer (5’ -> 3’)*

N

GTG ARA TGG TCA TGT GTG GCG G

CAR ATG TTA AAS ACA CTA TTA GCA TA

ACA GGT ACG TTA ATA GTT AAT AGC GT

ATA TTG CAG CAG TAC GCA CAC A

CAC ATT GGC ACC CGC AAT C

GAG GAA CGA GAA GAG GCT TG

Instructions for UseSARS-CoV-2 positive control is supplied as a lyophilized powder with nuclease-free water provided.

1. Spin down contents of tube.2. Add nuclease-free water :

3. Resuspend well and spin down contents before aliquoting.4. Use 1 μl SARS-CoV-2 positive control (100 pg) per reaction.

SARS-CoV-2 positive control 20SARS-CoV-2 positive control 100

20 µl100 μl

Reverse transcription (Superscript IV Vilo, Thermo Scientific) and qPCR (Sensifast SYBR No-ROX, Bioline) was performed in triplicates with six log-dilutions of SARS-CoV-2 positive control (starting amount: 73 ng). Primer sequences obtained from Corman, V. M et. al (2020) Detection of 2019 novel coronavirus (2019-nCoV) by real- time RT-PCR. Eurosurveillance. 25, 2000045 *R is G/A; S is G/C.