informal class evaluation 1. what do you like about the class? what works well for you? what should...
TRANSCRIPT
Informal Class Evaluation
1. What do you like about the class? What works well for you? What should we do more of?
2. What do you not like about the class? What doesn’t work for you? What should we do less of?
3. What are your thoughts on the labs so far (good / bad / indifferent)?
4. How could the class be improved for the rest of the term? What about for next term?
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Lab Debriefing
Why did the potato extract turn brown as it sat out on the desk?
What would you observe if the reaction was proceeding quickly? slowly?
Was the potato the enzyme? What was the substrate?
Enzyme Concentration
3
Time (min)
Substrate Concentration
4
Time (min)
Salt Concentration
5
Time (min)
Cell Structure and Compartments
The Cell Is the Basic Unit of Life
All living things are made of cell(s) (characteristic of life)
Cells
An organism can be unicellular or multicellular Range from one to
billions of cells Humans have over
200 types of cells
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Cells
An organism can be unicellular or multicellular Range from one to
billions of cells Humans have over
200 types of cells
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History of Cell Discoveries
1590’s: Hans and Zacharias Janssen Lens grinders First compound microscope
History of Cell Discoveries
1665: Robert Hooke Observed thin slice of
cork through microscope Tiny, hollow, roomlike
structures = “cells” (what monks live in)
Only saw outer walls because cork is dead
History of Cell Discoveries1680’s: Anton van
Leeuwenhoek Blood, rainwater,
scrapings from teeth, etc “Animalcules” =
unicellular organisms, bacteria?
History of Cell Discoveries1838-9: Schleiden and
Schwann Schleiden - plant parts
are made of cells Schwann - animal parts
are made of cells
CELL THEORY
1. Cells are the basic units of structure and function in all living things
2. All living things are made of cells
3. All cells come from pre-existing cells
How do we study cells?
Light Microscope Electron Microscope
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Light Microscopes (LM) Passes visible light through a specimen Can study living cells Can magnify only about 1,000 times Can resolve objects as small as 2 m
Light Microscope Pictures
Electron Microscope (EM)
Greater magnification than LM Uses a beam of electrons rather than light Has much greater resolution than LM (2
nm) Can magnify up to 100,000 times Cannot be used with living specimens
Scanning Electron Microscope (SEM) Studies detailed architecture of cell
surfaces
SEM PicturesPOLLEN
BLOOD CELLS
FISH GILLS
Transmission Electron Microscope (TEM) Details of internal cell structure
TEM Pictures
MELANOCYTE
NEURON
BACTERIA
BACTERIAE. coli400X
2m = 0.002 mm
BACTERIA
BACTERIAE. coliElectron microscope
ANIMAL CELL
HUMAN CHEEK CELLHomo sapiens400X
60m = 0.06 mm
ANIMAL CELL
HUMAN CHEEK CELLHomo sapiens600X
PLANT CELL
ELODEA LEAF CELLSElodea canadensis400X
Estimate size?
50 - 150 m = 0.05 - 0.15 mm
PLANT CELL
ELODEA LEAF CELLSElodea canadensis600X
Cell Types
Which two types of cells are more similar? Which type is more different?
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Prokaryotes and Eukaryotes Prokaryotes
Little internal organizationMuch smaller than
eukaryotes
Figure 5.2
Prokaryotes and Eukaryotes
EukaryotesDNA contained in
nucleus Membrane bound
organelles - internal compartments for special functions
Origin of Eukaryotes
ENDOSYMBIOSIS Large cells engulfed
smaller cells, but didn’t digest them
Origin of Eukaryotes
ENDOSYMBIOSIS Large cells engulfed
smaller cells, but didn’t digest them
MEMBRANE INFOLDING Cells folded membrane
in from outside of cell to increase surface area
Cell Size
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Cell Size
Most cells are microscopic
Some aren’t.
Cells Vary in Size
Minimum size = total size of all the molecules required for cellular activity
Cell Size
Minimum size = total size of all the molecules required for cellular activity
Maximum is limited by the need for sufficient surface area to carry out functions
Surface area to Volume Ratio A small cell has a greater ratio of surface area
to volume than a large cell of the same shape
Surface area to Volume Ratio
The microscopic size of most cells ensures a sufficient surface area across which nutrients and wastes can move to service the cell
Surface area to Volume Ratio
Microvilli in the small intestine
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Prokaryotes and Eukaryotes
Functions performed by an organism
Functions performed by an organism
Prokaryotes Unicellular The cell must perform
ALL functions Unspecialized
Eukaryotes Unicellular Multicellular Cells can be
specialized to perform one function
Cells interact together
Biological Hierarchy
Molecule Cell Tissue Organ Organ System Organism
Parts of the Cell
Cell ORGANELLES: Parts of the cell that perform a specialized function just like an organ in the body
Function #1: Keeping your insides inside
CELL MEMBRANE PROKARYOTES EUKARYOTES
Function 1b: Food, Waste, Gas Exchange
CELL MEMBRANEControls what
enters and leaves the cell
Function #2: Passing on your traitsGENETIC MATERIAL -
DNA
Function #2: Passing on your traitsGENETIC MATERIAL -
DNA PROKARYOTES
Circular Small
Function #2: Passing on your traitsGENETIC MATERIAL - DNA PROKARYOTES
Circular Small
EUKARYOTES Linear Large Enclosed in Nucleus
Function #3: Making Proteins
FUNCTIONS• Structures
– EX: red blood cells contain hemoglobin protein that carries oxygen
• Enzymes– EX: lactase digests
lactose sugar
Function #3: Making Proteins
PROKARYOTES: DNA contains
instructions to make proteins
Ribosomes assemble proteins
Function #3: Making Proteins
EUKARYOTES Contains different
organelles involved in protein synthesis
Function #3: Making Proteins
1. NUCLEUS Only Eukaryotes Contains DNA
Function #3: Making Proteins
1. NUCLEUS Surrounded by
“nuclear envelope” (membrane made of phospholipids)
Membrane contains “nuclear pores” that allow things in and out
Function #3: Making Proteins
2. RIBOSOMES All cells Assemble amino
acids to make a protein using the information from the nucleus
Function #3: Making Proteins
2a. FREE RIBOSOMES
Floating in cytoplasm Make proteins that will
be used in cytoplasm (hemoglobin)
Function #3: Making Proteins
2b. ATTACHED RIBOSOMES
Attached to Endoplasmic Reticulum
Make proteins that will be transported out of the cell
Function #3: Making Proteins
3a. ROUGH ENDOPLASMIC RETICULUM
Only Eukaryotes Site of protein
manufacturing and transport
Made of membrane
Function #3: Making Proteins3b. SMOOTH
ENDOPLASMIC RETICULUM
Synthesis of lipids to make vesicles (membrane sacks) that transport proteins to other organelles
Detoxification of drugs and poisons
Made of membrane
Function #3: Making Proteins
4. GOLGI APPARATUS Only Eukaryotes Sorts and tags proteins,
then packages them in vesicles for transport to final destination
Made of membrane Animation: Cain Ch 5
05
End-Product = Protein (EX: Hemoglobin) Used inside red
blood cells
Prokaryotes vs Eukaryotes
Which organelles involved in the eukaryotic process of protein synthesis are MISSING from prokaryotes?
Function #4: Getting rid of waste / recycling
PROKARYOTES Why did the surface
area to volume ratio have to be small?
Cell Membrane
Function #4: Getting rid of waste / recycling
5. LYSOSOME Only Eukaryotes Contain enzymes
that break down macromolecules
Made of membrane (stuff shipped in vesicles)
Function #5: Storing Extra Stuff
6. VACUOLE PLANTS – 1 large
vacuole Stores extra water
and nutrients
Function #5: Storing Extra Stuff
6. VACUOLE ANIMALS –
several smaller vacuoles
Stores mostly nutrients
Function #6: Moving
PROKARYOTES Flagella
Function #6: Moving
EUKARYOTES Flagella Cilia Pseudopods
Function #6: Moving
8. CILIA Small hairs
Function #6: Moving
8. CILIA Small hairs
9. FLAGELLA Whiplike tails
Function #6: Moving
Made of MICROTUBULES
Function #6b: Movement WITHIN the cell
7. CYTOSKELETON All cells Made of different
types of protein (recall how proteins are produced)
Function #6b: Movement WITHIN the cell
7a.MICROTUBULES Shape of cell Position organelles Vesicle guides
Function #6b: Movement WITHIN the cell
7a.MICROTUBULES Shape of cell Position organelles Vesicle guides
Function #6b: Movement WITHIN the cell
7b.INTERMEDIATE FILAMENTS
Structural reinforcement
Function #6b: Movement WITHIN the cell
7b.INTERMEDIATE FILAMENTS
Structural reinforcement
Flagella / cilia
All of these processes require…
Function #7: Making Energy10. CHLOROPLAST
Plants onlyCaptures energy from
sunlight and stores it as chemical energy in sugar (Photosynthesis
Chlorophyll = green pigment that captures energy
Some bacteria can do photosynthesis
Function #7: Making Energy11. MITOCHONDRIA
– Only Eukaryotes– Transform energy
stored in sugar into ATP
Function #7: Making Energy
PROKARYOTES Some can perform
photosynthesis All can perform
cellular respiration Lack chloroplasts
and mitochondria
Function #8: Protection, Support
13. CELL WALLPlants and BacteriaSupport and
protection for cell
Inside an Animal Cell
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Inside a Plant Cell
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Cell Parts Activity
1. Label the parts of the animal and plant cell.
2. Identify which is which (and how you can tell)
3. Fill in the table with functions of cell parts and analogies (CELL PART is like a WHATEVER because …)