The world leader in serving science

Dr. Timothy Cross

EMEA Regional Marketing Manager - HPLC

Increasing Productivity in Liquid Chromatography

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Benefits of increased productivity

Where can we find productivity gains?

Hardware enhancements

Column technology and chemistries

CDS software

Productivity in ion chromatography

Summary

Agenda Outline

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Benefits of Increased Productivity

Higher sample throughput

Faster from sample to result

Lower cost per sample

Enhanced qualitative and quantitative sample knowledge

Improved return on investments

Efficient bench space utilization

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Where Can Productivity Be Enhanced?

• System pump pressure

• Thermo Scientific™ Vanquish™ Duo system (Dual and Tandem LC / LC-MS)

• Consistent performance

Hardware

• Smaller column particles and diameters

• Novel chemistries

• Mass spectrometry friendly buffers

Columns and chemistries

• Thermo Scientific™ Chromeleon™ CDS software

• Thermo Scientific AppsLab Library of analytical applications

Software

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Productivity Enhancements - Hardware

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620 bar

Thermo ScientificTM

UltiMateTM 3000 Basic Automated

•Highly economic & reliable

Up to 1000 bar 1500 bar

Vanquish Horizon

•High pressure binary solvent mixing

•Industry-leading precision and accuracy

•Two thermostatting modes

•Unmatched detection sensitivity

•Biocompatible

•Integration of multiple detection technologies

Vanquish Flex

•High pressure binary and low pressure quaternary solvent mixing options

•Two thermostatting modes

•Biocompatible

•Integration of multiple detection technologies

Thermo ScientificTM

UltiMateTM 3000 SD

•Workhorse for standard HPLC applications

Thermo ScientificTM

UltiMateTM 3000 RS/BioRS

•Specialty workflow support

•Binary and quaternary UHPLC systems

800-1200 bar

Thermo ScientificTM

UltiMateTM 3000 RSLCnanoThermo Scientific™ EASY-nLC™ 1200

•UHPLC systems for Nano/Cap/Micro range

•Ideal front-end for proteomics applications

HPLC systems UHPLC systems

Routine analysis Low-flowFlexibility, more performance and method

developmentResearch & HT

Vanquish Duo workflows

Increasing System Pump Pressure

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Vanquish Horizon UHPLC Delivers – Boosted MS Performance

Standard method Sequence coverage

100%

100%

100%

100%

100%

Thermo Scientific™ Acclaim™ 120 C18 column, 2.1 x 250 mm, 2 - 45% ACN + 0.1% FA, 60℃.

5 10 15 20 25 [min]Time (min)

14.64

20.2310.592.73

10.78

14.52

7.992.70

6.97 9.43

5.20

1.86

4.456.02

3.351.13

3.124.12

2.431.08

30 min gradient

0.4 mL min-1

600 bar

20 min gradient

0.4 mL min-1

600 bar

13 min gradient

0.6 mL min-1

850 bar

8 min gradient

1.0 mL min-1

1150 bar

5 min gradient

1.1 mL min-1

1300 bar

• Vanquish system used for high throughput peptide mapping

• Gradient time reduced from 30 to 5 min with constant 100% sequence coverage

• Thermo Scientific™ Q Exactive™ HF hybrid quadrupole-Orbitrap mass spectrometer delivers enough data points per chromatographic peak

• Vanquish system is able to speed up peptide mapping significantly

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Three Workflows. Two Flow Paths. One Integrated UHPLC Solution.

Dual LC

• A two channel LC system with standard footprint

Tandem LC/LC-MS

• System for efficient detector utilization

Inverse Gradient

• System for advanced quantification capabilities

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Unique Vanquish Modules

▪ 2 ternary pumps in one housing

▪ 1000 bar (15,000 psi) version

▪ Delivers 2 completely independent

gradients

▪ 2 injection units in one autosampler

▪ 1000 and 1500 bar (15,000 or

22,000 psi) version

▪ Independent flow paths

Vanquish Flex

dual pump

Vanquish

dual split samplers

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Vanquish Duo for Dual LC

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What Can Be Accomplished With the Vanquish Duo for Dual LC?

Finished in half the time Efficient characterization

“The instrument offers a fantastic increase in throughputwhen running two different separation chemistries, enabling us to

generate large amounts of characterization data”

Dr. Jonathan Bones, NIBRT

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Key Benefits – Vanquish Duo for Dual LC

No additional bench space needed

Vanquish Duo for dual LC system Vanquish system

55 cm 55 cm

13

Key Benefits – Vanquish Duo for Dual LC

Lower costs per sample

Cost of ownership calculator:

Throughput increase

cost per sample

Aggregate analysis experimental conditions

Throughput gain

Doubled

throughput and

lower costs per

sample

Number of samples per day

96

192

Single Dual

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Vanquish Duo for Tandem LC/LC-MS

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Key Benefits – Vanquish Duo for Tandem LC/LC-MS

Increased throughput

Abs. RT Shift

Column left to right

[min]

Rel. RT Shift

Column left to right

[%]RT RSD [%]

n=10

Area RSD [%]n=10

0.023 0.18 0.11 2.47

Vanquish Duo for Tandem

5.0 10.0 15.0 20.0 25.0 30.0 35.0min

0

1.6e9

co

un

ts

1.6e9

Left column (n=5)

Right column (n=5)

co

un

ts

LC-MS peptide mapping example

0

10

20

30

40

50

60

70

80

90

2.1 mm - 50 mm 2.1 mm - 150 mm 2.1 mm - 250 mmTH

RO

UG

HP

UT

IN

CR

EA

SE

BY

(%

)

Throughput increase by Tandem LC/LC-MS

5 min Gradient 15 min Gradient 30 min Gradient

“Since installing the Tandem LC-MS system we have doubled our

throughput and increased the precision of our data!”

Richard Rogers, Just Biotherapeutics

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Key Benefits – Vanquish Duo for Tandem LC/LC-MS

Advanced column wash without sacrificing throughput

0

50

100

0 10 20 30 40 50 60

Elu

en

t B

[%

]

Run Time [min]

0

50

100

0 10 20 30 40 50 60

Elu

en

t B

[%

]

Run Time [min]

0

50

100

0 10 20 30 40 50 60

Elu

en

t B

[%

]

Run Time [min]

Time savings

Time savings

Standard gradient profile

Tandem mode allows for time savings

Extra column washing steps without

sacrificing throughput

Additional column wash

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Key Benefits – Vanquish Duo for Tandem LC/LC-MS

Lower costs per sample

Cost of ownership calculator:

Throughput increase

cost per sample

Long gradient experimental conditions

Throughput and

costs comparison

between standard

single and Vanquish

Duo workflows

Number of samples per day

24

48

Single Dual Tandem

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18

Vanquish Duo for Inverse Gradient

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• Detection of substances

without a chromophore

• Consistent analyte response

independent of chemical

structure

• Dynamic range up to four

orders of magnitude and

highest sensitivity

• Relative quantification using

virtually any standard

Unique Detectors: Charged Aerosol Detection

Comparison of charged aerosol detection

to UV and MS

Saving costs for additional detectors or assays

See what others are missing

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Conventional gradient elution

Inverse Gradient Compensation for Uniform Response

15%

85%

15%

Eluent dependent

analyte response

Identical mass o.c.Analytical gradient

Inverse gradient compensation

15%

85%

15%

Elu

en

t at d

ete

cto

r

Analytical gradient

50%

85%

15%

85%

Inverse gradient

Elu

en

t at d

ete

cto

r

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Key Benefits – Vanquish Duo for Inverse Gradient

0,0

0,1

0,2

0,3

0,4

0 10 20 30 40 50

Peak

Are

a (p

A*m

in)

Mass on column (ng)

Adenine

Tenofovir

Emtricitabin

Tenofovir Disoproxil

0

0,1

0,2

0,3

0,4

0 10 20 30 40 50

Peak

Are

a (p

A*m

in)

Mass on column (ng)

Adenine

Tenofovir

Emtricitabine

Tenofovir Disoproxil

Standard free quantification

Without gradient Compensation With inverse gradient compensation

Similar response curves for all analytes after gradient compensation

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Productivity Enhancements – Columns and Chemistries

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What is Thermo Scientific™ Accucore™ Vanquish Column?

• Next generation UHPLC column

• Founded on state-of-the-art core enhanced technology

• 1.5 µm solid core particles

• Designed in synergy with the Vanquish UHPLC to deliver:

• Better separations: High efficiency enables the separation of very complex mixtures

• More results: High efficiency at high flow rates

• Easier interactions: Seamless workflow solution, for simple and easy separations

Accucore Vanquish UHPLC columns

delivering powerful separations

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Why Core Enhanced technology Delivers: More Results

40% time saving and better separations

Accucore Vanquish C18 1.5 µm

100 x 2.1mm

Solid core C18 2.6 µm

100 x 2.1mm

Average increase in peak capacity of 220% Average increase in resolution of 24%

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Productivity Enhancements – Chromatography Data System (CDS) Software

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• Problem: Many areas where manual sequence creation causes errors

• Templates have limited flexibility

• Solution: eWorkflows make it simple for anyone to run powerful analyses

Application Automation – Chromeleon CDS eWorkflows

Select eWorkflow,

instrument and launch Set no. of samples

and start position

Click ‘Finish’

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Proven efficiency gains for lab productivity

• Significant productivity gains reported by range of customers from

different industries

• Productivity gains have been verified by direct competitor comparison

• Head to head comparison of same tasks (prescribed by customer) in each CDS

Chromeleon Chromatography Data System (CDS) – Proven Efficiency Gains

<50%Windows

<50%Mouse clicks

>50% Faster

Brian Alliston, Sterling Pharma“Having previously used several other CDS systems, Chromeleon proved to be a very intuitive

system. It has a ‘familiar feel’ which means it’s easy to learn. The user interface is simple and

clear and everything is where you would expect it to be. I find the system easy to use and

training new staff is easy.”

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AppsLab Library of Analytical Applications

Search applications

• Filter based on instrument, analyte or technique

Download eWorkflow

• Hundreds of eWorkflows

Run the eWorkflowin Chromeleon CDS

thermofisher.com/appslab

Find, download, and run

new methods in minutes

The world leader in serving science

Productivity Enhancements – Ion Chromatography

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The Thermo Scientific™ Dionex™ Ion Chromatography Product Line

RFIC

HPIC

Thermo Scientific™

Dionex™ Aquion™

system

Thermo Scientific™

Dionex™ Integrion™

system

Thermo Scientific

Dionex ICS-4000

system

Thermo Scientific

Dionex ICS-6000 system

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Introducing the ICS-6000 HPIC System: What’s New?

• Single or dual channel configurations

• Thermo Scientific™ IC PEEK Viper™ Fittings

• Thermo Scientific Dionex Consumables Device Monitor

• Unity Remote Services

• Tablet control of the IC system

• HPIC capable up to 5000 psi

• Automated eluent generation (RFIC-EG eluent

generation without manual eluent preparation))

• Always ready system operation with capillary IC

• Standard bore, microbore, and capillary formats

• Flexible detector options

Compatible with Thermo Scientific™ ISQ™ EC single

quadrupole, triple quadrupole, and high-resolution

accurate-mass (HRAM) Thermo Scientific™

Orbitrap™ mass analyzer mass spectrometers

Accelerate your

productivity

Explore the possibilities

IC – MS enabled

Solve complex analysis

challenges

• 2-D ion chromatography for trace-level analysis

• Improved method reproducibility for complex

carbohydrate analysis using Dual EGC control

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What is Consumables Monitoring?

• Automatically track consumables without human intervention

• Confirm installation

• Monitor usage

• Store and retrieve performance metrics

• Data are permanently stored on the consumable itself

• Assists with fulfillment of audit and documentation needs

• Increased awareness of usage and replacement frequency

• Elimination of manual log book entries

• Removes competitive lock-out

• Error prevention for new/novice IC users

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What is Consumables Monitoring?

• Assists with fulfillment of audit and documentation needs

• Increased awareness of usage and replacement frequency

• Elimination of manual log book entries

• Removes competitive lock-out

• Error prevention for new/novice IC users

Save a day of hunting down documentation = $500. Get a bonus of looking good and avoiding stress.

Plan ahead and avoid a day of downtime while awaiting consumables shipment. Potential savings: 32 samples at $50 each = $1600.

Eliminate 15 minutes/day = $1600/year. Have confidence that the recorded information is 100% correct.

Maintain productivity even when novice IC users replace consumables. Eliminate risk of destroying consumable. Potential savings: $1600/year

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Unity Remote Services

Diagnostics

• Predictive failure alerts

• Resource guidance

• Speed to resolution

Service

• Improves diagnostics

• Enhances technical support

• Increases first-time fix rate

Regulatory requirements

• Performance validation

• Quality validation

• Security

• Instrument history

• Audit trail of transactions

Product support

• Reduced diagnoses time

• Effective collaboration

Content delivery

• Instrument alerts

• Instrument performance

• Real time instrument status

NOTE: Remote Services not available in some areas

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Ultra

High

ProductivityLiquid

Chromatography

Summary

Productivity

• Increased throughput and uptime. Lower costs. Improved return-on-investment (ROI)

Increased Liquid Chromatography Productivity

• Combination of hardware, chemistry and software

Next Steps to Increased Productivity

• Discuss productivity solutions at booth 101 (Hall B1)

• Visit www.thermofisher.com/vanquishduo

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