improving post-rainy sorghum varieties to meet the growing ... · improving post-rainy sorghum...

Improving post-rainy sorghum varieties to meet the growing grain and fodder demand in India – Phase 2

(2013-2018)

Cape Town 11April, 2018 ACIAR project concluding meeting R Madhusudhana

HS Talwar, R MadhusudhanaJai Kishan, Shiwesh Kumar

ICAR-Indian Institute of Millets Research, Hyderabad, IndiaDO N

OT COPY

Role Of IIMR in Phase 2

Objective 1. Defining the best package to develop improved cultivars

1.1. Staygreen QTL (Stg3a and Stg3b) introgression into two post-rainy season genotypes.

1.2. Assess intermediate BC product for staygreen and trait expression in 2 post-rainy adapted genotypic background

1.3. Conduct multi-location trials of most promising products of all the six recurrent backgrounds

Objective 2. Improve breeding efficiency by better targeting key traits and trait donor.

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Target genotypes of post-rainy sorghum : productivity is ~700 kg/ha

Post-rainy sorghum crop under drought

CRS4RSLG262

(Phule Maulee)

Recurrent Pedigree Maturity (days) Yield (t/ha)

CRS4 Selection from (Chakur Bidri x SPV-1537) 3-1

110 (medium-deep soils)

Grain : 2.5-3.0Fodder :5

RSLG262 Germplasm selection 105-110 (Shallow-Medium soils)

Grain: :1.5-1.8 Fodder: 4.5-5 DO N

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B35: Stay-green trait donor

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B35 CRS4 RSLG262

Parents in Pre-bloom stage

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Crossing block: Development of Genetic material

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Available markers• Bhattramakki et al.

(2000)• Srinivas et al.(2008)• Yonemaru et al. (2009)

Newly designed • Stg3a : 37 (Stg3a:1-37) • Stg3b : 11 (Stg3b:1-11)

Development of SSR markers Stg3a : 57-62 Mb Stg3b: 69-72 Mb

Xtxp29857.1SBY-2-121257.3Stg3a_357.6Stg3a_557.7SBY-2-122558.0SBY-2-123858.3XSdre10858.4SBY-2-123958.5SBY-2-124558.9Stg3a_1359.0Unnhsbm271 SBY-2-124959.2SBY-2-125459.6SBY-2-125859.8Stgnhsbm3359.9SBY-2-126460.0msbCIR339 SBY-2-126760.2Stgnhsbm34 Xtxp214Xtxp44560.4SBY-2-127460.6XSdre2460.7SBY-2-127960.8Stgnhsbm44 SBY-2-128661.0Xtxp43061.1Stgnhsbm45 Stg3a_3061.2Xtxp161.3SBY-2-1293 XSdre32Unnhsbm15961.4Xtxp5661.5Stgnhsbm3561.6SBY-2-129861.8Stgnhsbm3661.9SBY-2-130462.0SBY-2-130862.2SBY-2-131262.4

SBY-2-144269.2Xtxp10069.5SBY-2-144569.6SBY-2-1448 Unnhsbm22969.9SBY-2-144970.0Xtxp20770.1SBY-2-145670.4Stg3b_870.5Stg3b_970.7SBY-2-1463 Xtxp29670.9SBY-2-146771.0SBY-2-147271.1Stg3b_1171.3

Red: Polymorphic Underlined : used for foreground selection

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RSL

G26

2 B

35

CR

S4

B35

RSL

G26

2 B

35

CR

S4

B35

3 F1’S

3 F1’S

4 F1’S

4 F1’S

SbGM2-6 SbGM4-4

Testing F1 hybridity : 5 SSRs usedCRS4 x B35 and RSLG262 x B35

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A H H H A H H A H HAHABHAHHHAAHHHAAAAAH

RSLG

262 B3

5

H H HA A AH H A H A H HA A A H AH A A A A H A A A A AH H HH H H H A HH H H H H A H A A HA

Foreground selection of RSLG262 BC1F1s with SBY-2-1472

CRS4

B3

5

B HA HH H HH H A A A H A H A A B A B H H A H A A A A A A H A A H H A B B A A H B A B H A H H A

A B A H H H H H H HHHHHAAAAAHHAHHAHHHHBForeground selection of CRS4 BC1F1s with SBY-2-

1279

Genotyping of BC1F1s : RSLG262 and CRS4

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Stg3a_50.0

SBY-2-125413.1SBY-2-125815.4SBY-2-126717.9SBY-2-127920.4Stg3a_3021.6Stgnhsbm36 SBY-2-129322.9SBY-2-130825.4SBY-2-131225.8SBY-2-130426.5

SBY-2-1448 Xtxp20737.5Xtxp296 SBY-2-1472Stg3b_9 Stg3b_1140.5

Stg3a_50.0

SBY-2-125811.1SBY-2-125411.9SBY-2-126712.7SBY-2-127914.8Stg3a_3016.0SBY-2-129317.4SBY-2-1312 SBY-2-130820.4

SBY-2-1448 Xtxp20738.8

Stg3b_9 Xtxp29653.2SBY-2-1472 Stg3b_1154.5

Xtxp29857.1SBY-2-121257.3Stg3a_357.6Stg3a_557.7SBY-2-122558.0SBY-2-123858.3XSdre10858.4SBY-2-123958.5SBY-2-124558.9Stg3a_1359.0Unnhsbm271 SBY-2-124959.2SBY-2-125459.6SBY-2-125859.8Stgnhsbm3359.9SBY-2-126460.0msbCIR339 SBY-2-126760.2Stgnhsbm34 Xtxp214Xtxp44560.4SBY-2-127460.6XSdre2460.7SBY-2-127960.8Stgnhsbm44 SBY-2-128661.0Xtxp43061.1Stgnhsbm45 Stg3a_3061.2Xtxp161.3SBY-2-1293 XSdre32Unnhsbm15961.4Xtxp5661.5Stgnhsbm3561.6SBY-2-129861.8Stgnhsbm3661.9SBY-2-130462.0SBY-2-130862.2SBY-2-131262.4SBY-2-144269.2Xtxp10069.5SBY-2-144569.6SBY-2-1448 Unnhsbm22969.9SBY-2-144970.0Xtxp20770.1SBY-2-145670.4Stg3b_870.5Stg3b_970.7SBY-2-1463 Xtxp29670.9SBY-2-146771.0SBY-2-147271.1Stg3b_1171.3

RSLG262 CRS4

Relative Physical and Genetic maps (BC1F1)

Local physical map

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QTL introgression into CRS4

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CRS4 x B35

F1 x CRS4

F2 x CRS4

Rabi 14-15:• 80 BC1F1s were sown in pots. (C1-80) • Genotyping of 80 BC1F1s was done with 15

(Stg3a and Stg 3b) markers• 11 selected plants were back crossed to

develop BC2F1s.

BC2F1

KH15: 59 BC1F2s were advanced to BC1F3s

Kharif 15:• 98 BC2F1 s from backcrossed progenies (C7, C41

and C47) were grown in pots.• FS: Genotyping completed with 15 Stg3a and

Stg3b markers • 11 plants based on genotyping data selected

and back crossed to develop BC3F1s.

BC1F2

BC3F1 Rabi 15-16:• 90 BC3F1s of C41-28 were grown in pots. • FS: Genotyping completed with 15 Stg3a and

Stg3b markers • 27 BC4F1s were developed in CRS4 ground.

• Background selection of BC3F1 plants (76) ; 839 markers tested.

Rabi 2015-16:66 BC1F3s developed

25 BC1F3s were evaluated in Physiology trials (Dr. Talwar )

Type (3A &3B)

Plants

BB 20BA 6A B 0

CRS4

BC1F1

x CRS4

x CRS4

BC3F2

BC1F3 BC2F2

BC1F4 BC2F3 BC3F2

BC4F1Kharif 16• 25 BC4F1s of C41-28 are grown in pots. • FS: Genotyping –going on

• To get BC4F2sKharif 16:• 100 BC3F2s of (C41-28-49 (40), C41-28-52

(30) and C41-28-75 (30)) are grown.• FS: Genotyping –Completed with 15 SSRs

47 BC1F4Kharif 2016 : Seed increase

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Kharif – 2016- BC3F2

•Three CRS4 derivatives selected based on FG and BG data

•C41-28-52 and C41-28-75 : HH (30 each)

•C41-28-49 : BB (40 plants)

•>90% recovery of RP

•Genotyping with 15 Stg QTL markers.

•Selected : B+B (20); B+A (6) and A+B (0)

Selfed to get BC3F3s.

Rabi season :2017-18

(BC3F4s)

Multi-location trials of selected MAS lines of CRS4

(Dr. H S Talwar)


Un-replicated trial (BC3F3)Advanced to BC3F4s

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OPY

CRS4 Foreground selection (BC3F1s)

POT

9

POT

13

POT

14

POT

15

POT

16PO

T 27

POT

28PO

T 34

POT

36

POT

38

POT

45

POT

52

POT

58PO

T 62

POT

63

POT

64

POT

67

POT

69

POT

70PO

T 71

POT

75

POT

78

POT

79PO

T 82

POT

85

POT

86

POT

90

POT

7PO

T 19

POT

30

POT

35

POT

39

POT

42PO

T 47

POT

60PO

T 81

POT

6PO

T 11

POT

12PO

T 22

POT

23PO

T 24

POT

31PO

T 32

POT

33

POT

43

POT

56PO

T 66

POT

68PO

T 83

POT

49

BC3F

2

F 2 F2 F2 F2 F2 F2 F2 F2 F2 F2 F2 F2 F2 F2 F2 F2 F2 F2

Stg3a

CRS4

B35

C28-

9

C28-

13

C28-

14

C28-

15

C28-

16C2

8-27

-1C2

8-28

C28-

34

C28-

36

C28-

38

C28-

45

C28-

52

C28-

58C2

8-62

C28-

63

C28-

64

C28-

67

C28-

69

C28-

70C2

8-71

C28-

75

C28-

78

C28-

79C2

8-82

C28-

85

C28-

86

C28-

90

C28-

7C2

8-19

C28-

30

C28-

35

C28-

39

C28-

42C2

8-47

C28-

60C2

8-81

C28-

6C2

8-11

C28-

12C2

8-22

C28-

23C2

8-24

C28-

31C2

8-32

C28-

33

C28-

43

C28-

56C2

8-66

C28-

68C2

8-83

C28-

49

Uni154 A B A A A A A H A A A H A A A H A A A H A A H H H A A A H A A H A A H A H H A H A A H H A H A A A A A H AXsdre55 A B G H H H H H A A H A H A A H H A A H A H H H A A A A H H H H A H A G H H A A A A A H A A A A A A H H AXtxp19 A B H H H H H H A H H H H H H H H H A H H H H H H H H A H H H H H H H H H H A A A A A A A A A A A A A A B

SBY-2-1254 A B H H H H H H A H H H H H H H H H H H H H H H H H H A H H H H H H H H H H A A A A A A A A A A A A A A B

SBY-2-1258 A B H H H H H H H H H H H H H H H H H H H H H H H H H A H H H H H H H H H H A A A A A A A A A A A A A A BSBY-2-1267 A B H H H H H H H H H H H H H H H H H H H H H H H H H A H H H H H H H H H H A A A A A A A A A A A A A A BSBY-2-1279 A B H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H A A A A A A A A A A A A A A B

Stg3a-30 A B H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H A A A A A A A A A A A H A A BSBY-2-1293 A B H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H A A A A A A A A A A A H A A BStgnhsbm36 A B H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H A A A A A A A A H H A H A A BSBY-2-1308 A B H H H H H H H H H H H H H H H H H H H H H H H H H H H H A H H H H H A H A A A H A A H H H H A H A A BSBY-2-1312 A B H H H H H H H H H H H H H H H H H H H H H H H H H H H H A H H H H H A H A A A H A A H H H H A H A A B

Xsdre45 A B H H H H H H H H H H H H H H H H H H H H H H H H H H H H A H H H H H A H A A H H A H H H H H A H A A BXtxp286 A B H H H H H H H H H H H H H H H H H H H H H H H H H H H H A H H A H H A H A A H H A H H H H H H H A A B

Stg3bSBY-2-1448 A B H H H H H H H H H H H H H H H H H H H H H H H H H H H A A H A A A H A A H H H H H H H H H H H H H H B

Xtxp207 A B H H H H H H H H H H H H H H H H H H H H H H H H H H H A A H A A A H A A H H H H H H H H H H H H H H BStg3b-9 A B H H H H H H H H H H H H H H H H H H H H H H H H H H H A A H A A A H A A H H H H H H H H H H H H H H Bxtxp296 A B H H H H H H H H H H H H H H H H H H H H H H H H H H H A A H A A A H A A H H H H H H H H H H H H H H B

SBY-2-1472 A B H H H H H H H H H B H H H H H A H H H H H H H H H H H A A A A A A A A A H H H H H H H H H H H H H H BStg3b-11 A B H H H H H H H H H B H H H H H A H H H H H H H H H H H A A A A A A A A A H H H H H H H H H H H H H H B

BC4F1 seeds 267 124 225 313 307 380 245 345 90 310 472 173 90 215 271 190 349 149 122 46 46 128 38 220

Seed Color YW W W W R W W W W W W W W W YW W W YW W W W R YW BDO NOT C

OPY

CRS4

C41-28-49-14B-B Plant

C41-28-49-18 B-A Plant

CRS4-BC3F2 plants

DO NOT C

OPY

QTL introgression into RSLG262

DO NOT C

OPY

RSLG262 x B35

F1 x RSLG262

F2 x RSLG262

Rabi 14-15:• 80 BC1F1s were sown in pots. • Genotyping of 80 BC1F1s was done with 15

(Stg3a and Stg 3b) markers• 13 selected plants were back crossed to

develop 13 BC2F1s.

BC2F1

Kharif15: 59 BC1F2s were sown and advanced to BC1F3s

Kharif15:• 90 BC2F1 s from backcrossed progenies (R33

and R37) were grown in pots.• FS: Genotyping completed with 15 Stg3a and

Stg3b markers • 11 plants based on genotyping data selected

and back crossed to develop 11 BC3F1s.

BC1F2

BC3F1Rabi 15-16:• 90 BC3F1s of R37-6 and R37-13 were grown. • FS: Genotyping completed with 15 Stg3a and

Stg3b markers • 33 BC4F1s were developed in CRS4 ground.

• Background selection of BC3F1 plants (73) ; 839 markers tested.

Rabi 2015-16:72 BC1F3s developed

25 BC1F3s were evaluated in Physiology trials (Dr. Talwar )

RSLG262

BC1F1

x RSLG262

x RSLG262BC1F3 BC2F2

BC1F4 BC2F3 BC3F2

Type (3A &3B)

Plants

BB 4BA 10A B 0

BC3F2 BC4F1 Kharif 15-16:• 36 BC4F1s of R37-6 (7) and R37-13 (29) in pots. • FS: Genotyping –going on

• To get BC4F2s

Kharif 16:• 148 BC3F2s of ( (R37-13-11-2 (50) and R37-13-30 (98)) are grown.• FS: Genotyping –going on

56 BC1F4Kharif 2016 : Seed increase

DO NOT C

OPY

Kharif – 2016- BC3F2

•Two RSLG262 derivatives selected based on FG and BG data

•R37-13-11-2 (50 plants) and R37-13-30 (48 plants) : HH

•95% recovery of RP

•Genotyping with 15 Stg QTL• markers.

•Select : B+B (4); B+A (10) and A+B (0)

•Selfed to get BC3F3s.


(BC3F4s)

Multi-location trials of selected MAS lines of

RSLG262(Dr. H S Talwar)


Un-replicated trial (BC3F3)Advanced to BC3F4s

DO NOT C

OPY

RSLG262

POT

91

POT

94

POT

98

POT

101

POT

103

POT

104

POT

107

POT

109

POT

111

POT

112

POT

113

POT

115

POT

125

POT

127

POT

130

POT

131

POT

134

POT

136

POT

137

POT

141

POT

150

POT

152

POT

153

POT

154

POT

154

POT

156

POT

156

POT

157

POT

157

POT

157

POT

158

POT

160

POT

161

POT

162

POT

164

POT

166

POT

167

POT

168

POT

168

POT

169

POT

170

POT

171

POT

173

POT

173

POT

175

POT

175

POT

176

POT

178

POT

92

POT

93

POT

99

POT

115

POT

117

POT

96

POT

96

POT

100

POT

110

POT

120

POT

121

POT

122

POT

123

POT

124

POT

126

POT

128

POT

129

POT

131

POT

132

POT

133

POT

135

POT

138

POT

139

POT

140

POT

143

POT

144

POT

145

POT

146

POT

148

POT

151

POT

155

POT

159

POT

161

POT

163

POT

165

POT

167

POT

169

POT

174

POT

174

POT

177

Marker

RSLG

262

B35

R6-1

R6-4

R6-8

R6-1

1

R6-1

3

R6-1

4

R6-1

7

R6-1

9

R6-2

1

R6-2

2

R6-2

3

R6-2

5-1

R13-

5

R13-

7

R13-

10

R13-

11-2

R13-

14

R13-

16

R13-

17

R13-

21

R13-

30

R13-

32

R13-

33R1

3-34

-1

R13-

34-2

R13-

36-1

R13-

36-2

R13-

37-1

R13-

37-2

R13-

37-3

R13-

38

R13-

40

R13-

41-2

R13-

42

R13-

44

R13-

46-1

R13-

47-1

R13-

48-1

R13-

48-2

R13-

49-2

R13-

50

R13-

51

R13-

53-1

R13 -

53-2

R13-

55-1

R13-

55-2

R13-

56

R13-

58

R6-2

R6-3

-2

R6-9

R6-2

5-2

R6-2

7-2

R6-6

-1

R6-6

-2

R6-1

0

R6-2

0

R6-3

0

R13-

1

R13-

2

R13-

3

R13-

4

R13-

6

R13-

8

R13-

9

R13-

11-1

R13-

12

R13-

13

R13-

15

R13-

18

R13-

19

R13-

20

R13-

23

R13-

24

R13-

25

R13-

26-1

R13-

28

R13-

31

R13-

35

R13-

39

R13-

41-1

R13-

43

R13-

45

R13-

47-2

R13-

49-1

R13-

54-1

R13-

54-2

R13-

57

stg3a

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

BC3F

2

Stg3a-5 A B H H H H H H H H H A H H H H H H H H H H H H H H H H H B H H H H H H H H H H H H H H H H H H H H H A A A A H H H H H H H H H H A H H H H H H H H H H H H H H H H H H H H H H H H

SBY-2-1254 A B H H H H H H H H H A H H H H H H H H H H H H H H H H H B H H H H H H H H H H H H H H H H H H H H A A A A A H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H



SBY-2-1279 A B H H H H H H H H H H H H H H H H H H H H H H H H H H H B H H H H H H H H H H H H H H H H H H H H A A A A A H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H

Stg3a-30 A B H H H H H H H H H H H H H H H H H H H H H H H H H H H B H H H H H H H H H H H H H H H H H H H H A A A A A H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H

SBY-2-1293 A B H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H A A A A A H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H

Stgnhsbm36 A B H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H A A A A A H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H



SBY-2-1312 A B H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H A A A H A H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H

Stg3b

SBY-2-1448 A B H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H A A H H H H H H H A H H H A H A H A A A A H H H A H A H A H A A H A A A A A A A

Xtxp207 A B H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A

Stg3b-9 A B H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A

xtxp296 A B H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H H A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A

SBY-2-1472 A B H H H H H H H H H H H H H H H H H H H H H H H H H H H H H B H H H H H H H H H H H H H H H H H H H H H H H A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A

Stg3b-11 A B H H H H H H H H H H H H H H H H H H H H H H H H H H H H H B H H H H H H H H A H H H H H H H H H H H H H H A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A H H A

281 444 305 333 453 500 163 126 300 290 335 510 445 134 133 274 366 451 411 248 264 246 250 242 308 157 250 275 328 426 348 565 355 464

W R R R B W W W W W W W W R W B W W R R R R W R R R R W R R R W W R

Foreground selection (BC3F1s)

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R37-13-20-21 B-B Plant

R37-13-11-2-26 B-A Plant

RSLG262-BC3F2 plants

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Status of MAS material available : IIMREntry Stage Lines

CRS4 BC1F4 22BC1F5 42BC2F3 90BC2F4 126BC3F4 510BC4F3 201

991

RSLG262 BC1F4 30BC1F5 54BC2F3 74BC2F4 69BC3F4 111BC4F3 192

530

Trial Stage Total CRS4 RSLG262MLT (HST) BC3F4 13 8 5ICRISAT BC3F4 123 74 49DO N

OT COPY

Phenotypic evaluations

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Phenotyping of stay-green introgressed BC products

1. Field evaluations:• 2016-17: Hyderabad• 2017-18: MLT

Hyderabad (Telangana)Solapur (Maharashtra)Bijapur (Karnataka)

2. Evaluations in Lysimeters at IIMR

Hyderabad

Solapur

Bijapur

Postrainy sorghum growing area

ACIAR project concluding meeting Cape Town 11April, 2018 H. S Talwar

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0

20

40

60

80

100

120

140

160

0

2

4

6

8

10

12

14

16

18

20

22

24

26

28

30

32

34

36

38

40

Jun1

1-17

,2016

1

8-24

2

5-01

Jul 0

2-08

0

9-15

1

6-22

2

3-29

3

0-05

Aug

06-1

2

13

-19

20-2

6

27

-02

Sep

03-0

9

10

-16

Sep1

7-23

24-3

0Oc

t 01-

07

08

-14

15-2

1

22

-28

29-0

4No

v 05-

11

12

-18

19-2

5

26

-02

Dec 0

3-09

10-1

6

17

-23

24-3

1Ja

n 01

-07

08-1

4

15

-21

22-2

8Ja

n 29

-04

Feb

05-1

1

12

-18

19-2

5

26

-4

Ma

r 05-

11

12

-18

19-2

5

26

-31

Rain

fall

(mm

)

Tem

pera

ture

(o C)

Standered weeks

Hyderabad

Rain fall (mm) Max T Min T

Total RF: 984.2 mm, Excess: 22%

8-12 oC

Weather at Hyderabad


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0

10

20

30

40

50

60

70

80

90

100

02468

101214161820222426283032343638404244

June

04-1

0Ju

n11-

17,20

16

18-

24

25-

01Ju

l 02-

08

09-

15

16-

22

23-

29

30-

05Au

g 06

-12

13-1

9

20

-26

27-0

2Se

p 03

-09

10-1

6Se

p17-

23

24

-30

Oct 0

1-07

08-1

4

15

-21

22-2

8

29

-04

Nov 0

5-11

12-1

8

19

-25

26-0

2De

c 03-

09

10

-16

17-2

3

24

-31

Jan

01-0

7

08

-14

15-2

1

22

-28

Jan

29-0

4

Fe

b 05

-11

12-1

8

19

-25

26-4

Mar 0

5-11

12-1

8

19

-25

26-3

1

Rain

fall (

mm

)

Tem

pera

ture

(o C)

Standered Weeks

Solapur

Rain fall (mm)

Max T

Min T

Weather at Solapur

Total Rainfall: 564.3, Excess: 3.5%


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MaterialWell watered Water stressed

BC3F4s of CRS4 & RSLG 262• 8 in CRS -4 background• 5 in RSLG 262 background

Traits : Phenology, growth parametersPhysiological traits (GLAR, RWC,

SPAD) Yield components (Grain yield

panicle weight, stover weight)

Experiments•3 Replications•2 Treat (WW & WS)•Split plot design


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Progeny MAS name Genotype Stg3a Stg3b

C1 C41-28-49-14 BB + +

C2 C41-28-52-18 BB + +

C3 C41-28-75-21 BB + +

C4 C41-28-52-10 BB + +

C5 C41-28-49-22 BB + +

C6 C41-28-52-13 BB + +

C7 C41-28-49-27 BA + -

C8 C41-28-49-18 BA + -

Progeny MAS name Genotpye Stg3a Stg3b

R1 R37-13-30-15,16 BB + +

R2 R37-13-30-11 BB + +

R3 R37-13-11-2-30 BA + -

R4 R37-13-11-2-26 BA + -

R5 R37-13-30-20, 37 AB - +

BC3F4 : Used for multiplication trials

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Soil Moisture

10

15

20

25

30

35

40

0 15 30 45 60 75 90 105 120 135 150

Soil

Moi

stur

e (%

)

Days after Sowing (DAS)

Hyderabad

Well watered (WW)

Water stressed (WS)

10

15

20

25

30

35

40

0 15 30 45 60 75 90 105 120 135

Soil

moi

stur

e (%

)

Days after Sowing (DAS)

Solapur


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Variations in green leaf area retention (GLAR) Location: Hyderabad

45.413

24.982

55.495

16.762

0

10

20

30

40

50

60

70

80

GLA

R (%

)

WS71.431

36.812

72.146

51.081

0

10

20

30

40

50

60

70

80

90WW

All QTL lines improved over recipient parents under both WS & WWMore improvement with RSLG 262 than CRS 4 under WS, vice-versa under WW Most improved lines under WS- CRS 4: C1, C3 &C7, RSLG 262- R5 & R3


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37.9

17.4

55.8

37.4

0

10

20

30

40

50

60

703A

+3B

(C3)

3A+3

B (C

4)

3A+3

B (C

1)

3A+3

B (C

2)

3A (C

8)

3A (C

7)

3A+3

B (C

6)

3A+3

B (C

5)

CRS

4

3A (R

3)

3A+3

B (R

2)

3A (R

4)

3B (R

5)

3A+3

B (R

1)

RSLG

262

M35

-1

B35

WS75.6

51.5

65.6

49.6

0

10

20

30

40

50

60

70

80

90WW

Variations in green leaf area retention (GLAR) Location: Solapur

All QTL lines improved over recipient parents under both WS & WWMore improvement with RSLG 262 than CRS 4 under WS, vice-versa under WWMost improved lines under WS- CRS 4: C3, C4 & C1; RSLG 262- R3, R2


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418.7

374.44390.74

371.48

0

50

100

150

200

250

300

350

400

450

500

3A+3

B (C

2)

3A+3

B (C

5)

CRS

4

3A (C

7)

3A+3

B (C

3)

3A (C

8)

3A+3

B (C

4)

3A+3

B (C

6)

3A+3

B (C

1)

3A+3

B (R

1)

RSLG

262

3A (R

4)

3B (R

5)

3A+3

B (R

2)

3A (R

3)

M35

-1

B35

WW

Variations in grain yield Location: Hyderabad

Five QTL lines improved in CRS 4, three lines in RSLG 262 under WS More improvement with CRS 4 (51%) than RSLG 262(23%) under WSMost improved lines under WS- CRS 4: C3 & C7 RSLG 262- R5, R1 & R3

297.96

197.78

344.26

279.44

0

50

100

150

200

250

300

350

400

450

Gra

in y

ield

(g m

-2)

WS


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598.06

549.81 534.63

0

100

200

300

400

500

600

700

3A (C

8)

CRS4

3A+3

B (C

6)

3A+3

B (C

3)

3A+3

B (C

4)

3A+3

B (C

1)

3A+3

B (C

2)

3A (C

7)

3A+3

B (C

5)

RSLG

262

3A (R

4)

3A+3

B (R

2)

3A+3

B (R

1)

3B (R

5)

3A (R

3)

M35

-1

B35

WW

Variations in grain yield Location: Solapur

Four QTL lines improved in both CRS 4, four lines in RSLG 262 under WS More improvement with CRS 4 (56%) than RSLG 262 (48%) under WSMost improved lines under WS- CRS 4: C3, C8,C7 RSLG 262- R3

129.3582.96

125.5684.81

0

100

200

300

400

500

600

700

Gra

in y

ield

(g m

-2)

WS


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997.6

740.7

875.6

666.9

0

200

400

600

800

1000

1200

3A+3

B (C

3)

3A+3

B (C

5)

CRS

4

3A (C

7)

3A+3

B (C

2)

3A (C

8)

3A+3

B (C

4)

3A+3

B (C

1)

3A+3

B (C

6)

3B (R

5)

3A+3

B (R

2)

RSLG

262

3A (R

3)

3A (R

4)

3A+3

B (R

1)

M35

-1

B35

WW

Variations in stover yield Location: Hyderabad

Three QTL lines improved in both CRS 4 & RSLG 262 under WS More improvement with CRS 4 (66%) than RSLG 262 (62%) under WSMost improved lines under WS- CRS 4: C3 & C7, RSLG 262: R5 & R2

758.9

457.4

637

393.7

0

200

400

600

800

1000

1200

Stov

er y

ield

9g

m-2

)

WS


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1453.7

907.4

1120.4

916.7

0

200

400

600

800

1000

1200

1400

1600

1800

2000

Stov

er y

ield

(g m

-2)

WS

1824.1

1407.41314.8

0

500

1000

1500

2000

2500 WW

Variations in stover yield Location: Solapur

One QTL lines improved in CRS 4, three lines in RSLG 262 under WS More improvement with CRS 4 (60%) than RSLG 262 (22%) under WSMost improved lines under WS- CRS 4: C3, RSLG 262- R2


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0.39

1.00

0.72

0.0

0.5

1.0

1.5

2.0

2.5

3A+3

B (C

1)3A

+3B

(C3)

3A (C

7)CR

S 4

3A+3

B (C

2)3A

+3B

(C6)

3A (C

8)3A

+3B

(C4)

3A+3

B (C

5)

3A+3

B (R

1)3B

(R5)

3A (R

3)3A

(R4)

3A+3

B (R

2)RS

LG 26

2

M35-

1B3

5

Drou

ght s

usce

ptib

le in

dex

(DSI

)

GLAR

0.06 0.060.13

0.97

0.0

0.5

1.0

1.5

2.0

2.5

3.0

3A+3

B (C

1)3A

+3B

(C3)

3A (C

7)3A

+3B

(C6)

3A+3

B (C

5)3A

+3B

(C2)

3A+3

B (C

4)3A

(C8)

CRS

4

3A (R

3)3B

(R5)

3A+3

B (R

1)3A

+3B

(R2)

RSLG

262

3A (R

4)

M35-

1B3

5

Grain Yield

0.13

0.38

0.53

0.0

0.5

1.0

1.5

2.0

2.5

3A+3

B (C

3)3A

+3B

(C1)

3A (C

7)3A

+3B

(C6)

3A (C

8)CR

S 4

3A+3

B (C

4)3A

+3B

(C5)

3A+3

B (C

2)

3A+3

B (R

1)3B

(R5)

3A (R

3)3A

+3B

(R2)

3A (R

4)RS

LG 26

2

M35-

1B3

5

Stover yield

Variations in drought susceptible index DSILocation: Hyderabad

CRS 4 - 3 QTL lines improved for GLAR, All in GY, 5 for SY. Most improved lines – C3, C1 & C7RSLG 262 – All QTL lines improved for GLAR, GY & SY. Most improved lines – R5, R1 & R3

DSI= (1-Viws/Viww)/D, D= (1-Viwsm/Viwwm)


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0.0

0.2

0.4

0.6

0.8

1.0

1.2

1.4

3A (C

7)3A

+3B

(C3)

3A+3

B (C

1)3A

+3B

(C5)

3A (C

8)3A

+3B

(C2)

3A+3

B (C

6)3A

+3B

(C4)

CRS4

3A (R

3)3A

+3B

(R1)

3A (R

4)3A

+3B

(R2)

3B (R

5)RS

LG 26

2

B35

M35-

1

GY

0.0

0.5

1.0

1.5

2.0

2.5

3A+3

B (C

6)3A

+3B

(C2)

3A+3

B (C

3)3A

(C7)

3A+3

B (C

4)3A

+3B

(C5)

3A (C

8)3A

+3B

(C1)

CRS

4

3A (R

3)3A

+3B

(R2)

3A (R

4)3A

+3B

(R1)

3B (R

5)RS

LG 26

2

M35-

1B3

5

STY

Variations in drought susceptible index DSILocation: Solapur

CRS 4 - Four lines improved for GLAR & GY, 5 for STY. Most improved lines – C3 & C7

RSLG 262 – All QTL lines improved for GLAR, GY & SY. Most improved lines – R3

DSI= (1-Viws/Viww)/D, D= (1-Viwsm/Viwwm)

0.0

0.5

1.0

1.5

2.0

2.5

3A+3

B (C

3)3A

(C7)

3A+3

B (C

1)3A

(C8)

3A+3

B (C

2)3A

+3B

(C4)

3A+3

B (C

6)3A

+3B

(C5)

CRS4

3A (R

3)3A

+3B

(R2)

3A (R

4)3B

(R5)

3A+3

B (R

1)RS

LG 26

2

M35-

1B3

5

Drou

ght s

usce

ptib

le in

dex

GLAR


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Evaluations in Lysimeters

Traits MeasuredWater extraction/Capture (Total WU)Transpiration efficiency

Lysimetery facility at IIMR – capacity 360cylindrical pots

Methodology Lysimters are PVC tubes(L-2m, Dia-30 cm)

filled with soil, kept in trenches

Crop was sown after saturating the soil(Thinned to one plant/tube)

After 30 DAS, open surface covered by 2”poly beads to cut evap.

Treatments: 2 (WW & WS )- 30DAS

Periodic weighing of tubes using load cellbalance on moving trolly


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Water extraction/capture

19350

17810

19100

14570

10000

12000

14000

16000

18000

20000

22000

24000

26000

3A+3

B (C

5)

3A (C

8)

3A (C

7)

3A+3

B (C

3)

CRS4

3A+3

B (C

6)

3A+3

B (C

4)

3A+3

B (C

2)

3A+3

B (C

1)

3A (R

4)

3A+3

B (R

1)

3A (R

3)

3B (R

5)

3A+3

B (R

2)

RSLG

262

M35

-1

B 35

Wat

er e

xtra

cted

(g p

lant

-1)

WS24170

2349023920

23380

10000

12000

14000

16000

18000

20000

22000

24000

26000WW

Improvement in water extraction in both backgrounds under WS. C3 & C7; R31.5 Kg/plant in CRS 4 and 4.5 kg/plant in RSLG 262 under WSNegligible improvement under WW


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Transpiration efficiency8.8

6.4

7.3

6.01

3.0

3.5

4.0

4.5

5.0

5.5

6.0

6.5

7.0

7.5

8.0

8.5

9.0

9.53A

+3B

(C3)

3A+3

B (C

2)

3A+3

B (C

4)

CRS

4

3A+3

B (C

1)

3A (C

7)

3A+3

B (C

6)

3A+3

B (C

5)

3A (C

8)

3B (R

5)

3A+3

B (R

1)

3A (R

3)

RSLG

262

3A+3

B (R

2)

3A (R

4)

M35

-1

B 35

Tran

spira

tion

effic

ienc

y (k

g w

ater

/g d

wt) WS

7.4

6.26.1

5.5

3.0

3.5

4.0

4.5

5.0

5.5

6.0

6.5

7.0

7.5

8.0

8.5

9.0

9.5WW

Improvement in TE in both backgrounds under both WS & WW (C3, C2; R5. R1 &R3) WS: 37% in CRS 4 and 21% in RSLG 262WW: 19% in CRS 4 and 11% in RSLG 262


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In sum! Staygreen QTL introgression led to significant improvement in

GLAR, GY, STY, WE and TE in both the genetic backgroundsparticularly under WS conditions

Yield components improved more in CRS 4 (Gy-54%, STY-63)than RSLG 262 background (Gy-35%, STY-47) under WS.

Enhancement in GLAR and yield components seems to be due toimprovement in WE and TE.

Improvement in WE was more in RSLG 262 than CRS 4 and viceversa for TE

Lines like C3 & C7 in CRS 4 & R3 in RSLG 262 backgrounds arepromising and need to be evaluated on more locations


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Improved lines over recipient parents

CRS 4: C3 (3A+3B) CRS 4: C7 (3A) RSLG 262: R3 (3A)


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Where to go??1. Multi-location testing for two more seasons to confirm the results2. Repetition of Lysimetry trials3. Fine mapping of Stg3a and Stg3b – candidate gene identification and

better markers for MAS4. On-farm trials of identified lines5. Registration of genetic stocks for TE and WE6. Introgression into new cultivars - CSV-29R (Medium- deep), CSV-26R

(shallow soils)

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Acknowledgements

RARS, BijapurCRS, Solapur

Jai KishanShiwesh kumar

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improving post-rainy sorghum varieties to meet the growing ... · improving post-rainy sorghum...

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