igem at william & mary...igem at william & mary caroline golino (cams, ’17) john marken...
TRANSCRIPT
![Page 1: iGEM at William & Mary...iGEM at William & Mary Caroline Golino (CAMS, ’17) John Marken (Mathematics, ‘17) Margaret Saha (Biology) “The new field of synthetic biology promises](https://reader035.vdocuments.site/reader035/viewer/2022081521/5e7b867669ae6532ae355f2d/html5/thumbnails/1.jpg)
iGEM at William & Mary
Caroline Golino (CAMS, ’17)
John Marken (Mathematics, ‘17)
Margaret Saha (Biology)
“The new field of synthetic biology promises to change health care, computer technology, the production of biofuels, and more. Students participating in the International Genetically Engineered Machine (iGEM) competition are on the front lines of this revolution.” [Cell, 26 Jan. 2017]
![Page 2: iGEM at William & Mary...iGEM at William & Mary Caroline Golino (CAMS, ’17) John Marken (Mathematics, ‘17) Margaret Saha (Biology) “The new field of synthetic biology promises](https://reader035.vdocuments.site/reader035/viewer/2022081521/5e7b867669ae6532ae355f2d/html5/thumbnails/2.jpg)
Genes consist of modular parts
PromoterRibosome
Binding Site
Protein Coding Region Terminator Icons:
Newcastle iGEM 2010
The design (or redesign) and construction of new biological entities such as enzymes, genetic circuits, and cells.
What is Synthetic Biology?
- - - - -
![Page 3: iGEM at William & Mary...iGEM at William & Mary Caroline Golino (CAMS, ’17) John Marken (Mathematics, ‘17) Margaret Saha (Biology) “The new field of synthetic biology promises](https://reader035.vdocuments.site/reader035/viewer/2022081521/5e7b867669ae6532ae355f2d/html5/thumbnails/3.jpg)
Genes (and their modular parts) can be wired into circuits
Environmental Signal
GeneticCircuit
Processing
CellularResponse
Genetic circuits can be coupled to cellular pathways
![Page 4: iGEM at William & Mary...iGEM at William & Mary Caroline Golino (CAMS, ’17) John Marken (Mathematics, ‘17) Margaret Saha (Biology) “The new field of synthetic biology promises](https://reader035.vdocuments.site/reader035/viewer/2022081521/5e7b867669ae6532ae355f2d/html5/thumbnails/4.jpg)
What is iGEM (international Genetically Engineered Machine)?
• iGEM maintains collection of standardized biological parts• iGEM hosts annual competition in synthetic biology• Students solve real world problems• Build genetically engineered biological systems
![Page 5: iGEM at William & Mary...iGEM at William & Mary Caroline Golino (CAMS, ’17) John Marken (Mathematics, ‘17) Margaret Saha (Biology) “The new field of synthetic biology promises](https://reader035.vdocuments.site/reader035/viewer/2022081521/5e7b867669ae6532ae355f2d/html5/thumbnails/5.jpg)
2014
![Page 6: iGEM at William & Mary...iGEM at William & Mary Caroline Golino (CAMS, ’17) John Marken (Mathematics, ‘17) Margaret Saha (Biology) “The new field of synthetic biology promises](https://reader035.vdocuments.site/reader035/viewer/2022081521/5e7b867669ae6532ae355f2d/html5/thumbnails/6.jpg)
2014 Summary
Submitted Channelrhodopsin-1 and Channelrhodopsin-2 in the BioBrick backbone to iGEM Registry
![Page 7: iGEM at William & Mary...iGEM at William & Mary Caroline Golino (CAMS, ’17) John Marken (Mathematics, ‘17) Margaret Saha (Biology) “The new field of synthetic biology promises](https://reader035.vdocuments.site/reader035/viewer/2022081521/5e7b867669ae6532ae355f2d/html5/thumbnails/7.jpg)
2014 Results
![Page 8: iGEM at William & Mary...iGEM at William & Mary Caroline Golino (CAMS, ’17) John Marken (Mathematics, ‘17) Margaret Saha (Biology) “The new field of synthetic biology promises](https://reader035.vdocuments.site/reader035/viewer/2022081521/5e7b867669ae6532ae355f2d/html5/thumbnails/8.jpg)
![Page 9: iGEM at William & Mary...iGEM at William & Mary Caroline Golino (CAMS, ’17) John Marken (Mathematics, ‘17) Margaret Saha (Biology) “The new field of synthetic biology promises](https://reader035.vdocuments.site/reader035/viewer/2022081521/5e7b867669ae6532ae355f2d/html5/thumbnails/9.jpg)
What is Noise?
Exp
ress
ion
Exp
ress
ion
Time
Time
![Page 10: iGEM at William & Mary...iGEM at William & Mary Caroline Golino (CAMS, ’17) John Marken (Mathematics, ‘17) Margaret Saha (Biology) “The new field of synthetic biology promises](https://reader035.vdocuments.site/reader035/viewer/2022081521/5e7b867669ae6532ae355f2d/html5/thumbnails/10.jpg)
Noise Measurement Results
pLlacO-1
pLac
λ pR
Time
![Page 11: iGEM at William & Mary...iGEM at William & Mary Caroline Golino (CAMS, ’17) John Marken (Mathematics, ‘17) Margaret Saha (Biology) “The new field of synthetic biology promises](https://reader035.vdocuments.site/reader035/viewer/2022081521/5e7b867669ae6532ae355f2d/html5/thumbnails/11.jpg)
2015 Results
Justin Knight, iGEM Foundation
![Page 12: iGEM at William & Mary...iGEM at William & Mary Caroline Golino (CAMS, ’17) John Marken (Mathematics, ‘17) Margaret Saha (Biology) “The new field of synthetic biology promises](https://reader035.vdocuments.site/reader035/viewer/2022081521/5e7b867669ae6532ae355f2d/html5/thumbnails/12.jpg)
![Page 13: iGEM at William & Mary...iGEM at William & Mary Caroline Golino (CAMS, ’17) John Marken (Mathematics, ‘17) Margaret Saha (Biology) “The new field of synthetic biology promises](https://reader035.vdocuments.site/reader035/viewer/2022081521/5e7b867669ae6532ae355f2d/html5/thumbnails/13.jpg)
Images adapted from Lestas et al 2010, Nature
Application of modular and additive control
![Page 14: iGEM at William & Mary...iGEM at William & Mary Caroline Golino (CAMS, ’17) John Marken (Mathematics, ‘17) Margaret Saha (Biology) “The new field of synthetic biology promises](https://reader035.vdocuments.site/reader035/viewer/2022081521/5e7b867669ae6532ae355f2d/html5/thumbnails/14.jpg)
Images adapted from Lestas et al 2010, Nature
Application of modular and additive control
![Page 15: iGEM at William & Mary...iGEM at William & Mary Caroline Golino (CAMS, ’17) John Marken (Mathematics, ‘17) Margaret Saha (Biology) “The new field of synthetic biology promises](https://reader035.vdocuments.site/reader035/viewer/2022081521/5e7b867669ae6532ae355f2d/html5/thumbnails/15.jpg)
Images adapted from Lestas et al 2010, Nature
Application of modular and additive control
![Page 16: iGEM at William & Mary...iGEM at William & Mary Caroline Golino (CAMS, ’17) John Marken (Mathematics, ‘17) Margaret Saha (Biology) “The new field of synthetic biology promises](https://reader035.vdocuments.site/reader035/viewer/2022081521/5e7b867669ae6532ae355f2d/html5/thumbnails/16.jpg)
Multi-State Response
Input
Ou
tpu
t
Modifying circuit behavior
![Page 17: iGEM at William & Mary...iGEM at William & Mary Caroline Golino (CAMS, ’17) John Marken (Mathematics, ‘17) Margaret Saha (Biology) “The new field of synthetic biology promises](https://reader035.vdocuments.site/reader035/viewer/2022081521/5e7b867669ae6532ae355f2d/html5/thumbnails/17.jpg)
Multi-State Response
Input
Ou
tpu
t
Shifting Sensitivity
Input
Modifying circuit behavior
![Page 18: iGEM at William & Mary...iGEM at William & Mary Caroline Golino (CAMS, ’17) John Marken (Mathematics, ‘17) Margaret Saha (Biology) “The new field of synthetic biology promises](https://reader035.vdocuments.site/reader035/viewer/2022081521/5e7b867669ae6532ae355f2d/html5/thumbnails/18.jpg)
Multi-State Response
Input
Ou
tpu
t
Scaling MagnitudeShifting Sensitivity
InputInput
Modifying circuit behavior
![Page 19: iGEM at William & Mary...iGEM at William & Mary Caroline Golino (CAMS, ’17) John Marken (Mathematics, ‘17) Margaret Saha (Biology) “The new field of synthetic biology promises](https://reader035.vdocuments.site/reader035/viewer/2022081521/5e7b867669ae6532ae355f2d/html5/thumbnails/19.jpg)
Multi-State Response
Input
Scaling MagnitudeShifting Sensitivity
InputInput
![Page 20: iGEM at William & Mary...iGEM at William & Mary Caroline Golino (CAMS, ’17) John Marken (Mathematics, ‘17) Margaret Saha (Biology) “The new field of synthetic biology promises](https://reader035.vdocuments.site/reader035/viewer/2022081521/5e7b867669ae6532ae355f2d/html5/thumbnails/20.jpg)
2016 Results
![Page 21: iGEM at William & Mary...iGEM at William & Mary Caroline Golino (CAMS, ’17) John Marken (Mathematics, ‘17) Margaret Saha (Biology) “The new field of synthetic biology promises](https://reader035.vdocuments.site/reader035/viewer/2022081521/5e7b867669ae6532ae355f2d/html5/thumbnails/21.jpg)
Broader Impacts of iGEM @ W&M:
• Ideas for new COLL courses
• Collaboration with W&M “Maker Movement”
• Synergy with W&M Engineering and Data Science
initiatives
• Outreach to local schools
• Partnerships with industry
The BioMakerSpace in ISC3
![Page 22: iGEM at William & Mary...iGEM at William & Mary Caroline Golino (CAMS, ’17) John Marken (Mathematics, ‘17) Margaret Saha (Biology) “The new field of synthetic biology promises](https://reader035.vdocuments.site/reader035/viewer/2022081521/5e7b867669ae6532ae355f2d/html5/thumbnails/22.jpg)
The Future of Synthetic Biology: Engineering a Biological Revolution …
Mali et al. 2013, ScienceGalanie et al. 2015, Science
Din et al. 2016, NaturePark et al. 2016, Science
Roybal et al. 2016, CellPardee et al. 2016, Cell