i ha - usda-aphis · aphis form 7023 (replaces vs form 18-23 (oct 88). which is obsolete part 1 -...

. . . - . . - . v -.-.,.,, - 1 - aDa (124 - r USDA*ARS*MOSQ AND FLY RSH UNIT; Page 2

NOU-24-2004 10:26 USDA QPHIS FIG Thls r8p* is maulred by leu (7 USC 2143), Fellun to regort aowmlng lo Ihe roguleUon6 can DEC ~7200p rltached fwm for n ~ u l l in w wdw lo came and dmkt end la Re tubjcct to wneHlee 89 pmvldrd b In 3ecUon 21! dalllanel Inronmllm.

UNITED STATES DEPARTMENT OF AGRICULTURE ANIMAL AND PLANT HEALTH INSPECTION SERVICE

ANNUAL REPORT OF RESEARCH FACILITY ( TYPE OR PRINT ) I Medical & Vet Entomology Res Lab

1600 Sw, 23rd Drive Gainesville, FL 32608

1. CERTIFICATE NUMBER: 58.F4006 CUSTOMER NUMBER: 945

I T-NG ~*wm ( uu OII k a d m u4 uhps W B I ~ ~ ~a~ued~or W d g , at aqmdmenteuon, or hold for fheee p u r n i r n nddU&ml ha I .- ) I

FORM APPROVED OM0 NO. 06794036

5 m . r k a e r u m . , Slngbrrd.

condlusntd, w ' hdd foruseln mchlnp, leeling. arperlmanu, ' research,or sum W( not ye used lor such purposes.

t?. Guinea Pigs

7, Hamsderri

12 Other Farm Animals

13. Other Animals __t_

FACILITY LOCATIONS ( Wm ) - Sw AMM ~sting

0. hmbw of enlmla upon whlch expanmlr, mcnfng, lblwrch. swgtry, w m s were FonducW Inwwlnp accompanying mln or dlrwbO b h e enlmsl!, an far whlch apptaprists 8fIe8u\Olle. Malgeclc, or l ~ ~ u l l i z l n g drupe were Udd.

NwnWr of wlmals upon Wlch IWcihg. srperWonts, f w d , MUOW of lesb mm, conbucled InwMnp lccomOenyIng pain or dkwaoe lo lh0 a i d s end lor nR1 \he uee at %roprlote enealheUc, anafgeelc, or Lrsnpulllz d w w l d have edwndy a f ? W me procedures, reo or hlmemllon ef #n (eechlng, remmh, expertmenb. s u r w , or &Is. ( An urplaruuon of the proccdm producing paln or dl6lreU In lhwe anlmls and he reeec such drug6 were not usad m a 1 be a w h d to 11119 rm

1 REWRI OF ANIMALS USED BY OR UNDER CONTROL OF RESE*RCH FACILITY f At&& addltlenal shedm If n ~ ~ s a l y or u6e APHIS Form 7- 1 I I 1 i

1

-

- C

- - - I

- - - -

- " - .. - - I

TOTAL. NUMBER OF ANIMALS

( COLUMNS C + D + E )

3) TMS lndlily 1s Jdherrng I0 the s l ~ W d 9 7 d -letlorn under Iha Ad, and II hno rcquirod lhel uctepllonr lo ma a@ndwas and ngukllms W apbdfled em mrd by ha prhdpel I-oc and ep krnl~l~nsl krlmel Care and UM Camnnoo (IACUC). A mmmy Mall ruclr uteutkn, lo rUachod to chk annual mpatt In add~aon la Idodvyhg me ~CUcgowovcd Q I C ~ O P ~ ~ . W srrmery Int Mer Wandon d ma src~0UoM as well ar me specks end mhbr of enltmh sffectsd.

4) The eaendlw wbrlnerreh for Ulls mew& bdllty fun d g m e t e auUmrily to Mswb the pfobldbn d adequete ve(orlnnry aue ud lo aversea the edequey of other eepcetr d enlnrel- und urn.

CERTIFICATION BY HEADQUARTERS RESEARCH FACILITY OFFICIAL ( Chlet Ereartlve dmcer or Legally ReeponslMe InetituUonal Ofndal )

OAT€ SI-0

APHIS 7023 (Roptawa VS FORM 18-29 (OCT 88). wNCh I6 obsew.) nbuqy I

This report is required by law (7 USC 2143). Failure to report according to the regulations can See reverse side for Interagency Report Control No I result in an order to cease and desist and to be subject to penalties as provided for in Section 2150. additional information. 01 80-DOA-AN

MAIL CODE 810-3 KENNEDY SPACE CENTER, FL 32899

I 3. REPORTING FACILITY (List all locations where animals were housed or used in actual research, teslng, teaching, or experimentation, or held for these purposes. Attach additional

ANNUAL REPORT OF RESEARCH FACILITY (TYPE OR PRINT)

J sheets if necessary.) I FACILITY LOCATIONS(sites)

LlFE SCIENCES SUPPORT FACILITY KENNEDY SPACE CENTER, FL 32899

FORM APPROVED OM8 NO. 0579-0036


2. HEADQUARTERS RESEARCH FACILITY (Name and Address, as registered wifh USDA, indude zig, Code)

SPACE LlFE SCIENCES LAB SLSL BLDG M6-1025; STATE ROAD 3 8 FIFTH ST

Animals Covered By The Animal

Welfare Regulations

1. REGISTRATION NO. CUSTOMER NO. 58-F-0009 966

animals being bred, conditioned, or held for use in teaching. testing, experiments, research, or surgery but not yet used for such purpo=.

animals upon which teaching. research. experiments, or tests were conducted invdving no pain, distress, or use of pain- relieving drugs.

which experiments, teaching, research. surgery, or tests were conducted invdving accompanying pain or distress to the animals and for which appropriate anesthetic, analgesic, or tranquilizing drugs were used.

4. Dogs

5. Cats I I I

6. Guinea Pigs I I I 7. Hamsters 1 I I

9. Non-Human Primates

8. Rabbits

10. Sheep

I

1 I. Pigs I I I

12. Other Farm Animals

13. Other Animals

experiments, research, surgery or tests-were conducted invdving accompanying pain or distress to the animals and for which the use of appropriate anesthetic,analgesic, or tranquilizing drugs would have adversely affected the procedures, results, or interpretation of the teaching, research. experiments, surgery, or tests. (An explanation of the pmcedures pmducing pain or distress in these animals and the reasons such drugs were not used must be attached to this repott)

F.

TOTAL NO. OF ANIMALS

(Cols. C + D + E)

-

ASSURANCE STATEMENTS

1) Professionally acceptable standards governing the care, treatment. and use of animals, including appropriate use of anesthetic, analgesic, and tranquilizing drugs, prior to, during. and following actual research, teaching, testing, surgery, or experimentation were followed by this research facility.

2) Each principal investigator has considered alternatives to painful procedures.

3) This facility is adhering to the standards and regulations under the Act, and it has required that exceptions to the standards and regulations be specified and explained by the principal investigator and approved by the Institutional Animal Care and Use Committee (IACUC). A sum- of all the exceptions is attached to thk annual report. In addition to identifying the IACUC-approved exceptions. this summary includes a brief explanation of the exceptions, as well as the species and number of animals affected.

L - I ,

I a

APHIS FORM 7023 (Replaces VS FORM 18-23 (Oct 88). which is obsolete PART 1 - HEADQUARTERS (AUG 91 )

4) The attending veterinarian for this research facility has appropriate authority to ensure the provision of adequate veterinary care and to oversee the adequacy of other aspects of animal care and use.

CERTIFICATION BY HEADQUARTERS RESEARCH FACILITY OFFICIAL (Chief Executive Officer or Legally Responsible Institutional official)

I c e t i i that the above is true, correct, and complete (7 U.S.C. Section 2143) SIGNATURE OF C.E.O. OR INSTITUTIONAL OFFICIAL NAME a TITLE OF C.E.O. OR INSTITUTDNAL OFFICIAL (Type or Print) DATE SIGNED

I 1012612W

result in an order to cease md dadst and to be subkd to 'ml t ies as bwided f;;r in Saction 21!


1. CERTIFICATE NUMBER: 5843400 1

CUSTOMER NUMBER: 873

FORM AFVROV& OM8 NO. 0579-0036

wov 2 3m ANNUAL REPORT OF RESEARCH FACILITY

( TYPE OR PRINT )

Florida State University Laboratory Animal Resources 101 Biomedical Research Facility Mail Code 4341 Tallahassee, FL 32306

I. REPORTING FACILITY ( List all locations Mere animals were housed or used in actual research, tes I, or experimentation, or held for these purposes. Altach additional sheets if necessary ) I FACILITY LOCATIONS ( sites ) - See Atached Listing

REPORT OF ANIMALS USED BY OR UNDER CONTROL OF RESEARCH FACILITY I Attach additional sheets H necessanf or use APHIS Form 7023A 1 1 I I

C. Numberd animals upon which teaching, research. experiments, or t e s t s m amducted involving no pain, distress, or use a paindieving drugs.

D. Number d animals upon which W m e n t s , teaching, research,

E. Number d anlmals upon which teaching, experimenb. research, suqety or tests were conduded involving sccompenying pain a distress to the animals and for wh the use d appnwiate aneslhetic, analgesic, w tranquilit drugs would have adversely affected the procedures, res or MemMath d lh teaching, mearch, ewedmenls. surgery, or tests. ( An explanation of the procedures produdng pain or distress in these animals and the reasc such drugs wen, not used must be altached to this reporl

F.

TOTAL NUMBER OF ANIMALS


B. Number*nimsl being e.. conditioned, or held for usei in teaching. testing. experiments. research, or swgery but not yc used for such purposes.

A n h h Cwered By The Animal

Wolfam R.guIation8 conducted Invo(ving rcoonpwrylng w n or dlsbess to the animals an forwlrichrppm9rkto anWhetk. analgesic, or banquilidng drugs were used.

6. Guinea Pigs I I

9. Non-human Primates I I 10. Sheep

11. Pigs I

12. O h r F a n Animals I I

1 ASSURANCE STATEMENTS

13. Other Animals

Meadow Voles

Prarie Voles

I) f w e r s i o ~ l l y mxptabk standards g o w h g tho ram, wamt, wd use d d d s . induding appmpwa use of neststic, rnrlgasic. .nd tranquilizing drugs, prior to, dwkrg. and fdkming -1 teaching. testing, wrgery, a experimentation were followed by thk research facility.

120 --.----

440

2) Each principal investigator has considered altmatims to painful procedures. 0

3) T M facility h adhering to the atwrdards a d regdaths under the Act. md it has requlred that exu&Mns to the standards a d regulations be spedfied and explained by the prindpel investigator a d aP kwtiMional Animal Can, a d Use ComrniRw (UCUC). A summary of dl such axcoptiom b attached to thk 8 m l npon In addition to idenWying the I A C U C a m exceptions. this summary im brief ewlanation d the exceptions. as well as the species and number d animals affected.

4) the attending veterinarian for this research facility has .ppropri& auth4ty b enwe the provision d idequate vsterlnrry and to ovwsee the adequacy d other aspects d animal care a d ube.

CERTlFK%TK)N BY HEADQUARTERS RESEARCH FACKlM OFFICIAL ( Chbf tcecrrthre Officer or Legally Responsible I-l Offidal )

DATE SIGNED

1 1 / 2 2 / 0 4 SIGNANRE OF C.E.O. OR INSTrmTK)NM OFFICIAL

APHIS FORM 7023 (Replaces VS FORM 18-23 (& 88). which is obsdae.)

NAME 6 TITLE OF C.E.O. OQ INSTfnmONAL OFFICIAL ( Type or W )

. This repnn 1s required by law I7 USC 2143). Fallureto report according lo lhs reguiationscan N OV 3 0 20° See atlached~mfor Interagency Repan Controi No.: -sZ-

result in an order to ceaseand d e w and to be sublea lo pensltiesal provided fwin Section 21: additional i n f m l i m .

I I Telephone: (352) -392-9271 I ANNUAL REPORT OF RESEARCH FACILITY

(TYPE OR PRINT )

I I I 3. REPORTING FACILW ( ~ i s t ali locations where antmats ware housed or used in mluai raseanh, tasting. or expedmantation, or hold for thaw puma-. Anach additional rheelo if necessary) I

FORM APPROVE0 OM0 NO 0579-0036


university Of Florida P.O. Box 115500 Gainesville. FL 32611

FACILITY LOCATIONS ( Sites I - See Alached Listing

1. CERTIFICATE NUMBER: 5&R-0003


REPORT OF ANIMALS USED BY OR UNDER

8. Number danlmsl being bred. candil~oned w

Animals Corerad held for use in By Th. A n l ~ l leaching, test~ng,

WetIan Regulations expsnmsnts. research. or aurgev but not ye wed for Ouch

NTROL O F RESEARCH FACILITY I Attach addltlonal shaeo H n.cesurv or u u APHIS Form 7023A I I . Number of D. Number of animals upm

animals w o n I which exoerimts.

4. Dogs

5 Cats

tesb were

8 Rabbits

9. Non-human Primates

10. Sheep

mnduaed inmlving no pato. distre58,or use o pam~l ieang dwge.

11. pigs 1 0

13. Other Animals

dmt- to the enimal. an f~ uhish appmpma aneslhetlc, analgesic, or tranquil~ring drugs were used.

! COl - C

- -

- -

- -

- -

- -

- -

- -

- - - -

Deer Mice I 0 ASSURANCESTATEMENTS I

I 1 Prdesionally acceptable rlsndsrdr governing me cars. trsatmont. and use d animals. including appmpnets uu, of mestalk, analgesic. and tranquilizing drug*, p m to, during, and foiloxrng actual rest teaching, testing, surgery. or expenmentation were f o l l ~ e d by mir resoarch facility.

2) Each principal mvesligator has considered alternativer to pamful pmedures

surgery, or teats. (An explanation of the pmcedurss prcduuw pain ix distress in thee animals and the reas such dwgs wen n d used mug be ambled lo this repon

4) The anending veterinar~an for lhis rerearch facility has appmprime wthonty to enrun the prowan d adequate vslmnary care and to ovaroes the adequacy ofolhsr aspecls d snimsi care and use

I CERTIFICATION BY HEADQUARTERS RESEARCH FACILITY OFFICIAL ( C h i d Executive OfRatr or Lagally Reaponslble InstlMional OfRclal ) I


OATE SiGNED

APHIS FORM 7023- (Replacer VS FORM 18-23 (OCT 86) uhlch 8s obsolete )

Facilities under the care of Animal Care Services housing USDA-covered species:

University of Florida P.O. Box 100006 Campus Gainesville, FL 326 10 (352) 392-2978

Health Sciences Center (Communicore Bldg., Progress Center Facility, Shands, Medical Science Center, Academic Research Bldg.) - Animal Care Services 1600 SW Archer Road Gainesville, FL - Shands Complexes 1351 Research Drive Alachua, FL -Progress Center

34'h Street Farm - Animal Care Services SW 34Ib Street Gainesville, FL 32610

Animal Research Facility (ARF), including deer paddocks on Mowry Road - Animal Care Services Bldg 167 Mowry Road, Gainesville, FL 32610

McKnight Brain Institute - Animal Care Services 100 S. Newell Drive, Gainesville, FL 32611

College of Veterinary Medicine - Metabolic Bldg. 2015 SW 16& Avenue, Gainesville, FL 32610

College of Liberal Arts and Sciences - Bartram Hall Bartram Hall, NeweU Drive, Gainesville, FL 32611

College of Liberal Arts and Sciences - Psychology Bldg. Psychology Building, Center Drive, Gainesville, FL 32611

Bivens Arm Research Facility on 23rd Street - Animal Care Services 2292 SW 23rd Street Gainesville, FL

Bldg. 628, Physiology Barn and Surgical Suite, Bldg. 459, rooms 137 and 137 A-C -Animal Sciences, Gainesville, FL

10. Dairy Research Unit, Gainesville, FL 13200 NW 59Ib Drive, Gainesville, FL 32653

11. Paddock 1 and Paddock 7, Gainesville, FL College of Veterinary Medicine, 2015 SW 16" Avenue, Gainesville, FL 32611

12. Whitney Marine Lab, St. Augustine, FL 9505 Ocean Shore Boulevard, St. Augustine, FL 32080

13. Food Science and Human Nutrition Building, Bldg 475 Newell Drive, Gainesville, FL 32611-0370

Column E Explanation

This form is intended as an aid to completing the Column E explanation. It is not an official form and its use is voluntary. Names, addresses, protocols, veterinary care programs, and the like, are not required as part of an explanation. A Column E explanation must be written so as to be understood by lay persons as well as scientists.

1. Registration Number: 58-R-0003

129 2. Number of animals used in this study.

3. Species (common name) Rabbits of animals used in the study.

4. Explain the procedure producing pain and/or distress.

T i t l e : D277; Determinat ion o f e f f i c a c y o f smallpox vaccine LC16m8 i n the Rabbi tpox-rabbi t model.

Procedure: Please see attached.

5. Provide scientific justification why pain andlor distress could not be relieved. State methods or means used to determine that pain and/or distress relief would interfere with test results. (For Federally mandated testing, see Item 6 below)

Please see attached.

6. What, if any, federal regulations require this procedure? Cite the agency, the code of Federal Regulations (CFR) title number and the specific section number (e.g., APHIS, 9 CFR 113.102):

Agency CFR

Protocol # D277, Rabbits

4. Explanation of procedure:

New Zealand White rabbits are being utilized as animal models for these infections. This project characterizes the genes required for the systemic disease caused by rabbitpox. Rabbitpox is lethal to rabbits, while vaccinia, which is over 95% identical at the gene level to rabbitpox, causes only a local lesion and is used as a vaccine. The goal of this experiment is to identify the genes required for the systemic infection of rabbitpox in order to have potential targets for antiviral therapies in the treatment of other human pathogenic poxviruses such as smallpox and monkeypox. Since the differences in pathogenesis cannot be seen in cell culture the use of animal models is necessary.

The first part of this work (experiment #1) examines the dosage and verifies the differences in disease caused by vaccinia and rabbitpox virus in rabbits. Specifically, the project re-determines the dose of rabbitpox virus that causes systemic disease and verifies that comparable or higher doses of vaccinia virus do not cause systemic disease. The critical part of experiment #1 will be to determine whether rabbits infected with mixtures of vaccinia and rabbituox viruses develop systemic disease, with accompanying secondary lesions typical of rabbitpox. This last experiment mimics the clinical situation in which humans are vaccinated after exposure to smallpox. These results help determine effective vaccine regimens. The experiments require minimum manual restraint of the rabbits, shaving of both hind flanks and the intradermal inoculation of lOOul buffer containing the corresponding plaque forming units of virus, either alone or as a mixture. Following infection, animals are routinely monitored two times a day (early morning and late afternoon), until or unless symptoms of disease develop. Upon onset of symptoms, the animals are closely observed four times a day (early morning, mid-day, late afternoon and late evening) for signs of severe disease (as indicated by decreased respiratory rates, dyspnea, open-mouth breathing, severe lung sounds, temperature equal to or exceeding 106"F, anorexia (not to exceed four days), dehydration, andlor morbidity). At this point the animals are euthanized according to the AVMA Panel on Euthanasia. A set of criteria for scoring the level of disease in the animals has been created. Upon euthanasia, the animals undergo necropsy to examine the spread of the virus to secondary sites and for the isolation of viruses for further characterization.

The dominance of rabbitpox virulence upon infections with mixtures of vaccinia and rabbitpox viruses provides a system for the identification of the rabbitpox virus genes, which particularly dispose the virus to cause systemic disease in rabbits. Identification of such genes provides the possibility of developing antiviral drugs and immunological reagents against these specific virulence determinants.

Experiment #2 is designed to infect rabbits with mixtures of recombinant vaccinia and rabbitpox viruses in order to identify rabbitpox virus genes associated with

systemic disease. Recombinant viruses from cell culture are isolated and then used to infect rabbits. The recombinants capable of causing systemic disease can then be isolated from the secondary lesions typical of systemic disease. In the event virulence of rabbitpox virus in mixed infections of vaccinia and rabbitpox viruses is not dominant, and is attenuated during mixed infections, then specifically engineered vaccinia virus containing segments of rabbitpox virus will be constructed, purified and assayed in rabbits for increased systemic disease. The experiment requires minimum manual restraint of the rabbits, shaving of both hind flanks, and the intradermal inoculation at up to five sites on the flanks of lOOul buffer containing the recombinant viruses. Following infection, animals are routinely monitored two times a day (early morning and late afternoon), until or unless symptoms of disease develop. Upon onset of symptoms, the animals are closely observed four times a day (early morning, mid-day, late afternoon and late evening) for signs of severe disease (as indicated by decreased respiratory rates, dyspnea, open-mouth breathing, severe lung sounds, temperature equal to or exceeding 106OF, anorexia (not to exceed four days), dehydration, and/or morbidity). At this point the animals are euthanized according to the AVMA Panel on Euthanasia. Upon euthanasia the animals undergo necropsy to examine the spread of the virus to secondary sites and for the isolation of viruses for further characterization.

Experiment #3 represents an alternative method for identification of rabbitpox virus, which predisposes the virus to virulence. The third set of experiments is to replace the identified gene segments of rabbitpox responsible for virulence with the corresponding genes in vaccinia and assay for attenuation after the injection of the recombinant viruses into rabbits. Attenuation of this engineered rabbitoox virus - strain is recognized by the appearance of a local lesion with no accompanying systemic disease. Following infection, animals are routinely monitored two times a day (early morning and late afternoon), until or unless symptoms of disease develop. Upon onset of symptoms, the animals are closely observed four times a day (early morning, mid-day, late afternoon and late evening) for signs of severe disease (as indicated by decreased respiratory rates, dyspnea, open-mouth breathing, severe lung sounds, temperature equal to or exceeding 106"F, anorexia (not to exceed four days), dehydration, andor morbidity). At this point, the animals are euthanized according to the AVMA Panel on Euthanasia. Upon euthanasia the animals undergo necropsy to examine the spread of the virus to secondary sites and for the isolation of viruses for further characterization.

5. Justification:

Analgesics are not indicated in these studies because their effects on the inflammatory and immune responses hinder the distinctions observed in the two viral diseases. It is critical that the rabbitpox and vaccinia viral diseases be distinguishable. For the screening of recombinant viruses it is also critical for the disease pathways to be uninhibited for the detection of virulent viruses.




57 2. Number of animals used in this study.

3. Species (common name) Rabbits of animals used in the study

4. Explain the procedure producing pain andlor distress.

T i t l e : D290; Gene r e g u l a t i o n o f mammalian DNA v i ruses .

Procedure: Please see attached.

5. Provide scientific justification why pain andlor distress could not be relieved. State methods or means used to determine that pain andlor distress relief would interfere with test results. (For Federally mandated testing, see Item 6 below)

Please see attached.

6. What. ~f any, federal regulations require this procedure? Cite the agency, the code of Federal Regulations (CFR) title number and the specific section number (e.g., APHIS. 9 CFR 113.102):

Agency CFR

Protocol # D290, Rabbits

4. Explanation of procedure:

New Zealand White rabbits are being utilized as animal models in the study. This is an experiment to determine efficacy of the smallpox vaccine LC16m8 against rabbitpox.

Proposed experiments in rabbits: 1. Determining the lethal dose of rabbitpox in rabbits.

2. Determining efficacy of the smallpox vaccine LC16m8 against rabbitpox in rabbits.

Experiment 1 determines the dosage of rabbitpox required to lethally infect rabbits. This requires manually restraining the rabbits and infecting them with rabbitpox by intraderma1 injection.-i he site ofinoculation will be a shaved region of each hind flank. Following infection, animals will be routinely monitored two times a day (early morning and late afternoon) until symptoms of disease develop. Upon onset of symptoms (see criteria below), the animals are closely monitored four times per day for signs of severe disease (as indicated by decreased respiratory rates of 25 bpm or less, difficulty breathing, open-mouth breathing, severe lung sounds, temperature equal to or exceeding 106'F, loss of appetite [not to exceed four days], and dehydration) at which point the animals are humanely euthanized.

A set of criteria for scoring the level of disease in the animals has been created to determine the time when the project shifts monitoring from 2 to 4 times a day and to determine when euthanasia is performed.

Experiment 2 determines the efficacy of LC16m8 against lethal rabbitpox challenge in rabbits. Dryvax and placebo are also being tested as positive and negative controls, respectively. This requires vaccinating the rabbits and then subjecting them to challenge by infecting them with rabbitpox. Briefly, the rabbits are vaccinated by scarification and are challenged with rabbitpox intradermally at day 28. Challenge is being done at day 28 since it is the average time at which - - - neutralizing antibodies are at peak titers in response to a vaccine.

Considering that this is the first time that vaccinia-based vaccines are being tested for their abilities to neutralize rabbitpox, it is necessary that a range of concentrations of challenge rabbitpox be used in the study. This circumvents the possibility that could occur if we only used one concentration of challenge rabbitpox, where the chosen concentration simply overwhelms even an efficacious vaccine. Therefore, the vaccine-challenge study is done using the two lowest lethal concentrations of rabbitpox (referred to as "lowest lethal pfu" and "second lowest lethal pfu," respectively) as determined in Experiment 1.

As detailed above, the animals are closely observed, up to four times a day, using the guidelines outlined in the first experiment above, for signs of severe disease (as indicated by decreased respiratory rates of 25 bpm or less, difficulty breathing, open-mouth breathing, severe lung sounds, temperature equal to or exceeding 106OF, loss of appetite (not to exceed four days), and dehydration, at which point the animals are humanely euthanized.

For endpoint analyses, blood is drawn from vaccinated rabbits at baseline, day 14 and 28 (pre-challenge) to measure neutralizing antibodies against rabbitpox in vitro. Also, internal organs are excised from a specific number of rabbits from each vaccine arm at day 38 (i.e., 10 days post-challenge, last day of the experiment) to determine the influence of vaccination on challenge rabbitpox titer in these organs.

5. Justification:

Rabbitpox is a lethal infection in rabbits. The rabbits will be vaccinated with LC16m8, Dryvax, or placebo. They are then injectedscarified with rabbitpox to determine whether vaccination protects the rabbits against a lethal rabbitpox infection. Therefore, providing these animals with any other form of anesthesia, analgesia or treatment will not be appropriate. It is necessary for the project to determine whether the animals develop severe symptoms, as that is the only way to tell whether the vaccine treatment is working. Presumably, if they "don't" develop severe symptoms, the vaccine is working as expected.

Thts report is required by law (7 USC 2143). Failure to report according to the regulations can 'OV I- 52@k Wattachedfonnfor Interagency Report Control NO- additional infonetion. 1 result in an order to cease and desist and to be subiect to ~enalties as d d e d f& in Section 21!


1. CERTIFICATE NUMBER: 58-R-0005 I FORM APPROVED OMB NO. 0579-0036


Mount Sinai Medical Center Mount Sinai Medical Center 4300 Alton Rd. Miami Beach, FL 33140

ANNUAL REPORT OF RESEARCH FACILITY ( TYPE OR PRINT )

Telephone: (305) -674-2790

-

3. REPORTING FACILITY ( List all locations where animals k r e housed or used in actual research. testing, or experimentation, or held for these purposes. Attach additional sheets if necessary )

1 REPORT OF ANIMALS JSED BY OR UNDER CONTROL OF RESEARCH FACILITY I Attach additional s h t s if ~essaimr or use APHIS Form 7023A 1 1 B. Number d animal

being bred, conditioned, or held for use in teaching, testing. experiments, research, or surgery but not yo used for such purposes.

C. Number of animals upon which teaching, research. experiments, or tests were conducted involving no pain. distress, or use cr pain-relieving drugs.

--

D. Number of animals upon which experiments, teaching, research, surgery. or tests were conducted involving accompanying pain a distress to the animals an for which appropriate amthetic, analgesic, or tranquilizing drugs were used.

E. Number of animals upon which teaching, experiments, march , surgery or tests were conducted involving accompanying pain or distress to the animals and for wb the use of appropriate anesthetic, analgesic, or tranquiliz drugs would have adwKsel y affected the procedures, res a int6fpretaUon of the teaching, research, expe8iments, surgery, or tests. ( An explanation of the procedures producing pain or dlstrsss in these animals and the reasc such drugs were not used must be attached to this repod


Animals Covmd By The Animal

Welfare Regulations ( COLUMNS C + D + E )

4. Dogs

5. Cats

6. Guinea Pigs I -- I 7. Hamsters I -- I 8. Rabbits I -- I 9. Non-human Primates

10. Sheep

12. ~Other Farm Animals s 13. Other Animals r

ASSURANCE STATEMENTS I 1) RofeSsionally acceptable standards governing the care. treatment. and use of animals, including appropriate use of anestetic. analgesic, and tranquilizing drugs, prior to, during, and following actual rese

teaching, testing, surgery, or experimentation were followed by this research facility.


3) This facility is adhering to the standards and regulations under the Act. and it has required that exceptions to the standards and regulations be spedfied and explained by the principal i nve~ t iga t~ and aP Institutional Animal Care and Use Committee (IACUC). A summary of all such rxceptionr b attached to thk annual npoh In addition to identnying the IACUC-approved exccceptims. this Summary in( brief explanation of the exceptions, as well as the species and number of animals affected.

4) The attending veterinarian for this research facility has appropriate authority to ensure the provision d adequate veterinary care and to oversee the adequacy of other aspects of animal care and we.

CERTIFICATION BY HEADQUARTERS RESEARCH FACILITY OFFICIAL ( Chief Executive Officer or Legally Responsible Institutional Official )

( AUG 91 )

7 ATE SIGNED

///y/'z/ #

APHIS FORM 7023 (Replaces VS ~ 0 ~ ~ 1 8 - 2 3 (OCT 88). which is obsolete.) I

This rspc,w required by law (7 U6C 2143) Failurnto mpon am din^ tothe repulat8ons can Sot nveras side 01. oi8o.BoA-AN lntela sncy Rapan Control No

, ~esun in an order to mass and desist and to be subjen to penanies as pmvidedfor m Ssdion 21 50 addtionel infomation

ANNUAL REPORT OF RESEARCH FACILITY I I DFf

UNlTEO STATES DEPARTMELIT OF AGRICULTURE ANIMAL AND PLANT HEALTH INSPECTION SERVICE

I 3. ~mpomn. f m d ~ ~ t y (~is1.11 i o u ~ ~ ~ . m e n .nim.ls wn hsusM a u u d to estu.~ nm.rrh. ~esung. or held (or mew punrose. . AN* mio on.^ .nee* (n n-.su/) I

FAClTlLlTY LOCATIONS (Sites) - See Anached Listing

I. CERTIFICATE NUMBER: SCR.OO07


I REPORT OF ANIMALS USED BY OR UNDER

FORM APPROVED OMB NO. 057%00PS I

A. 8. Number af animals being

Animals Covered bred B ~h ~ n i a1 mnditio ed

wel%re ReguEtiona or held &t h e m teaching.

............................ 12. UOR 13. Other i ~ a t by rprc+s) not eyused

for uch Dumoses. . .

4. Dogs

5. Cak

6. Guinea Pigs

7. Ham9ters

8. Rabbits

9. Non-human Primates 99

10. Sheep

11. Pigs


INTROL OF RESEARCH FACILITY (Attach a itional sheets if necessary or use APHIS FORM 7023A) I

I ASSURANCE STATEMENTS I

CERTIFICATION BY HEADQUARTERS RESEARCH FACILITY OFFICIAL (Chief ExecuUve Offlcer or Legally Responsible Instltuuonal OMclal)

+ (Repiaces FORM 18-23 (OCT 88), which is obsolete.)

(AUG 91)

OFFICIAL,Typo apnnt)

DATE SIGNEO

ATTACHMENT 1 - Facility Locations

University of Miami ani Contact person for all facilities:

Rosentstiel Medical Science Building 1600 NW 10 Avenue Miami, F133136

R Bunn Gantier Building 1011 NW 15 Street Miami, FL 33136

Diabetes Research Institute (DM) 1450 N W 10Avenue Miami, FL 33136

Parkinson Foundation Building 1501 NW 9 Avenue Miami,FL 33136

Bascom Palmer Eye Institute (BPEI) 900 NW 17 Street Miami, FL 33136

Rosenstiel School of Marine & Atmospheric Science (RSMAS) 4600 Rickenbacker Causeway Key Biscayne, FL 33149-1098

South Campus 12500 SW 152 ST Miami, FL 33177

Perrine Primate Center 15657 SW 127 Avenue Perrine, FL 33177

Behavioral Medicine Research Building Department of Psychology 125 1 Stanford Drive Coral Gables, FL 33146

Cox Science Bldg. (182) 1301 Memorial Drive Coral Gables, FL 33124

Lois Pope Life Center (LPLC) 1095 NW 14th Terrace Miami. FL 33136

Batchelor Children's Research Institute (BCRI) 1580 NW 10 Avenue Miami, FL 33136

Mannheimer Foundation, Inc. (Satellite Facility) 20255 S.W. 360" Street Homestead, Florida 33034-4102

ATTACHMENT 2

PROTOCOLS REQUIRING SPECIAL EXEMPTION FROM RECOMMENDED POLICIES OR GUIDELINES

IACUC reviewed, discussed and approved the following exemptions from USDA Policy.

Protocol #01-078 - Granted an exemption for not using analgesia in Hamsters and Rabbits for antibody production with Freund's adjuvant. The IACUC reviewed, discussed and approved the exemption fiom USDA Policy. Protocol #01-173 - Exemption granted for Non Human Primates (NHPs) for IV injection and subsequent hydration for 2-12 hours. Animals are observed continuously and provided additional environmental enrichment while restrained. Protocol #02-241 - Exemption granted for pig restraint by sling for a total of 5 hours. Animals are observed continuously while restrained. Protocols #02-257, 03-130 and 03-131 - Exemption granted for restraining board restraint for NHPs for 1 hour for IVGTT and 2-12 hours for STZ injections or critical care as needed. Animals are observed continuously and provided additional environmental enrichment while restrained. Protocol #03-069 - Granted an exemption for not using analgesia in Rabbits for antibody production with Freund's adjuvant. The IACUC reviewed, discussed and approved the exemption from USDA Policy. Protocol #03-222 - Exemption granted for Multiple Survival Surgery and for unrelieved social stress. Protocol #04-127 - Exemption granted for rabbit antibody production with Freund's adjuvant. The IACUC reviewed, discussed and approved the exemption for USDA Policy. Protocol #04-171 - Exemption granted for streptozotocin injection: the animal will be placed on the restraining board, injected with ST2 and dehydration for 2 hours post injection. The animal will be placed in a cage, and 8-14 hours after the injection - when hypoglycemia occurs-the monkey will be placed on the restraining board for an additional 2-12 hours to administer glucose and maintain normoglycemia. Metabolic challenges: (glucose, arginine, glucagons) the monkey will be sedated with ketamine, placed on restraining board and maintained there for up to 1-2 hours to collect samples. Thyrnoglobulin fludarabine administration: The first dose of thymoglobulin is administered over a 4 hour period, the 2nd over a 3 hour period and subsequent doses over a 2 hour period. Each antibody is considered in the context of known side effects of administration (or lack thereof). Mabs are given over a time period up to 1 hour that prevents documented side effects in NHP or humans. Animals are observed continuously and provided additional environmental enrichment while restrained. IACUC Committee approved an exemption to individually house a rhesus monkey with individually housed cynomologus monkeys. Common infectious agents have been ruled out and compatibility has been established.

10. Exemptions for MULTIPLE SURVIVAL SURGERY PROCEDURES were reviewed, discussed and approved by the IACUC for the following protocols involving a PIG model of skin injury.

01-01 I-ENTITLED: To examine the effect of various topical agents on incisional wounds in a porcine model

01-012: ENTITLED: A wound healing model to examine the effect of various agents on skin graft take and wound healing

01-102: ENTITLED: An animal model to examine the effect of various topical agents, dressings 01-102resub10,Ol-102resub11: ENTITLED: An animal model to examine the effects of various topical agents, dressing and/or devices on second degree burn wound healing

01-103: ENTITLED: An animal model to examine the effect of various topical agents, dressing 01-103resub13,Ol-103resub14: ENTITLED: An animal model to examine the effect of various topical agents, dressings and/or devices on partial thickness wound healing

01-124: ENTITLED: An animal model to study various topical agents, dressings and/or devices on partial thickness wound infections 01-124resub20: ENTITLED: Evaluation of an antimicrobial dressing on the proliferation of pseudomonas aeruginosa in a partial thickness wound model 01-124resub21: ENTITLED: Evaluation of an antimicrobial dressing on the proliferation of Staphyloccuc aureus in a partial thickness wound model 01 -124resub22,01-124resub23,01-124resub24: ENTITLED: An animal model to study

01-2 13: ENTITLED: Photosensitive PEG-heparin Hydrogels in Wound Healing

02-001: ENTITLED: A New Model of Pediatric Stroke in Infant Piglets 02-170: ENTITLED: Development of a lymphedema-Wound Healing Model in Pigs

02-249: ENTITLED: An animal model to study various topical agents, dressings, andlor devices on bacterial biofilms on partial thickness wound infections 02-249resub05: ENTITLED: Staphylococcus aureus biofilms in atopic dermatitis? 02-249resub06: ENTITLED: Evaluation of an antimicrobial dressing on the proliferation of pseudomonas aeruginosa in a partial thickness wound model 02-249resub06: ENTITLED: Evaluation of an antimicrobial dressing on the proliferation of staphylococcus aureus in a partial thickness wound model 02-249resub07: ENTITLED: An animal model to study various topical agents, dressing, andfor devices on bacterial biofilms on partial thickness wound infection 03-047: ENTITLED: An animal model to examine the effect of various topical agents, dressing and/or devices on scar formation in deep partial thickness wounds 03-086 ENTITLED: Laser Resurfacing: study to examine the effect of different test agents on laser resurfacing healing 03-162 ENTITLED: An animal model to study various topical agents, dressing and/or devices on second-degree burn wound infections.

03-162resub01,03-162resub02: ENTITLED: An animal model to study various topical agents, dressing andlor devices on second-degree bum wound infections. 04-038: ENTITLED: An Animal study to determine the effects of various topical treatments and/or dressings on full thickness wounds in a porcine model 04-038resub01: ENTITLED: An Animal model to determine the effects of various topical treatments and/or dressing on full thickness wounds in a porcine model 04-039: ENTITLED: A porcine model to study the effect of various topical wound healing and/or antimicrobial agents on deep partial thickness wounds 04-080 (01-102): ENTITLED: An animal model to study the effects of various topical agents, dressings andfor devices on second degree bum wound healing 04-081 (01-103) ENTITLED: An animal model to examine the effects of various topical agent, dressings, and/or devices on partial thickness would healing 04-089: ENTITLED: To Determine the effects of Ischemia, Hypoxia, and Microbes on Wound Healing using a porcine model 04-101: ENTITLED: Carotid aneurysm model 04-1 16 (01-124): ENTITLED: An Animal model to study various topical agents, dressing and/or devices on partial thickness wound infections

11. Exemptions for MULTIPLE SURVIVAL SURGERY PROCEDURES were reviewed, discussed and approved by the IACUC for the following protocols involving Canine models

01-1 10: ENTITLED: Studies of the Graft promoting, tolerogenic potential of donor cells andlor antigens administered in conjunction with novel imrnunomodulatory drugs in a canine islet transplant model: Effect of transplant site, donor cell composition, and timing. .. 02-089: ENTITLED: A novel Artificial Spinchter for the Control of Urinary Incontinence 02-264: ENTITLED: Microfabricated deliverylrepositioning system for stents

03-019: ENTITLED: Evaluation of new coil designs in canine

04-049: ENTITLED: Bioengineering of a Stent-Graft for Gene Therapy

04-101 : ENTITLED: Carotid aneurysm model

04-137: ENTITLED: Alternatives Sites for Islet Implantation in Dogs

12. Exemptions for MULTIPLE SURVIVAL SURGERY PROCEDURES were reviewed, discussed and approved by the IACUC for the following protocols involving Non Human Primate Models

01 -061 : ENTITLED: Transplantation of porcine transgenic OEG after primate spinal cord injury

01-173: ENTITLED: Multifaceted approaches to the induction of tolerance to allogeneic and xenogeneic pancreatic islets in non-human primates: Incorporation of novel immunointervention agents, administration of donor antigen and encapsulation technology 02-107: ENTITLED: A Breeding Center for Specific Pathogen Free Macaques (Macaca mulatto) 02-257: ENTITLED: Targeted radiotherapy with a bone-seeking radioistop compound (153 samarium-lexidronam) to establish hematopoietic chimerism and tolerance in non- human primate islet allograft recipients 03-067: ENTITLED: Autologous transplantation of olfactory ensheathing glia into the primate cervical spinal cord 03-130: ENTITLED: Use of an implantable device for xenogeneic pancreatic islet cotransplants with irnmunoprotective sertoli cells in non-human primates 03-131: ENTITLED: Gene modification of pancreatic islets for enhancement of post- transplant survival in non-human primates. 03-158: ENTITLED: Transplantation of Bone Marrow Stromal Cell after Primate Spinal Cord Injury 04-1 71 : ENTITLED: Pancreatic Islet Transplantation in Non Human Primates: Multificeted Approaches to the Induction of Tolerance Enhancement of Engrahent

13. Exemptions for MULTIPLE SURVIVAL SURGERY PROCEDURES were reviewed, discussed and approved by the IACUC for the following protocols involving Rabbit models

00-127: ENTITLED: Ex-Vivo expansion of limbal epithelial stem cells on amniotic membrane 01-201 : ENTITLED: Regulated therapeutic angiogenesis: Preclinical Studies 02-065: ENTITLED: Ex-vivo Expansion of Rabbit Retinal Pigment Epithelial Cells on Rabbit Amniotic Membranes for Autologous Re-Transplantation onto Denuded Bruch's Membrane 02-223: ENTITLED: Stenting and coiling of elastase-induced aneurysms 02-254: ENTITLED: Genetic engineering of vascular prosthetic grafts to prevent restenosis 03-039: ENTITLED: In vivo evaluation of an embolic containment system in a rabbit, elastase-induced 03-1 08: ENTITLED: Preliminary investigations of endovascular therapies for the treatment of aneurysms 03-151: ENTITLED: Stent-assisted coiling of elastase induced carotid aneurysms in rabbits using a new self-expanding helical stent design 03-180: ENTITLED: Fibered and no-fibered platinum coils for the treatment of elastase-induced carotid aneurysms in rabbits-Evaluation of stroke

03-188: ENTITLED: Ex-vivo Expansion of Limbal Epithellial Stem Cells on Amniotic Membrane 03-214: ENTITLED: Stenting and Coiling of Elastase-Induced Aneurysms 03-222: ENTITLED: Biobehavioral Base of CHD Risk and Management (Program Project) Project 3: Environment, CNS and Atherosclerosis in WHHL Rabbits 04-002: ENTITLED: Baseline Ultrasound and MRI Imaging of Periocular Anecortavd Acetate Drug Administration in Rabbit Eyes 04-020: ENTITLED: Flow Divertors to Cure Cerebral Aneurysms 04-040 ENTITLED: Development of a Lymphedema Wound Healing Model in Rabbits 04-105 ENTITLED: Preliminary investigations of endovascular therapies for the treatment of aneurysms 04-149 ENTITLED: Coiling of elastase-induced carotid aneurysms in rabbits using a new bioactive coli design 04-190 ENTITLED: Endovascular treatment of elastase-induced carotid aneurysms in rabbits using a new liquid embolic agent

14. Exemptions for MULTIPLE SURVIVAL SURGERY PROCEDURES were reviewed, discussed and approved by the IACUC for the following protocols involving cat models 02-134: ENTITLED: Laser trabeculoplasty using Titanium Sapphire and Alexandrite vs Argon lasers 03-046: ENTITLED: Continuous intraocular pressure sensing system

15. Exemptions for MULTIPLE SURVIVAL SURGERY PROCEDURES were reviewed, discussed and approved by the IACUC for the following protocols involving guinea pig models 02-222: ENTITLED: Neural receptor enhancement project

16. Exemptions for MULTIPLE SURVIVAL SURGERY PROCEDURES were reviewed, discussed and approved by the IACUC for the following protocols involving goat models

03-082: ENTITLED: Vena cava filter study

Optional Column E Explanation Form

This form is intended as an aid to completing the Column E explanation. It is an official form and its use is voluntary. Names, addresses, protocols, veterinary care programs, and the like, are not required as part of an explanation. A Column E explanation must be written so as to be understood by lay persons as well as scientists.

I . Registration Number: 58-R-0007

2. Number of animals used in this study. 2

3. Species (common name) of animals used in this study.Hamster


Production of polyclonal antibodies using Freunds Complete Adjuvant- no pain relief.

5. Provide scientific justification why pain andlor distress could not be relieved. State methods or means used to determine that pain andlor distress relief would interfere with test results. (For Federally mandated testing, see question 6 below)

Analgesia was withheld because the use of analgesics and antiinflammatory agents could blunt the antibody response to the injected antigen. The unrelieved distresdpain is justijied by the need to maximize the immune response to the new antigen.

6. What, if any, federal regulations require this procedure? Cite the agency, the Code of Federal Regulations (CFR) title number and the specific section number (e.g., APHIS, 9 CFR 113.102): none.

Agency CFR

Optional Column E Explanation Form

This form is intended as an aid to completing the Column E explanation. It is an official form and its use is voluntary. Names, addresses, protocols, veterinary care programs, and the like, are not required as part of an explanation. A Column E explanation must be written so as to be understood by lay persons as well as scientists.

1. Registration Number: 58-R-OW7

2. Number of animals used in this study. 1

3. Species (common name) of animals used in this study.Rabbit


Production ofpolyclonal antibodies using Freunds Complete Adjuvant- no pain relief.

5. Provide scientific justification why pain and/or distress could not be relieved. State methods or means used to determine that pain andlor distress relief would interfere with test results. (For Federally mandated testing, see question 6 below)

Analgesia was withheld because the use of analgesics and antiinflammatory agents could blunt the antibody response to the injected antigen. The unrelieved distresdpain is justijied by the need to maximize the immune response to the new antigen.

6. What, if any, federal regulations require this procedure? Cite the agency, the Code of Federal Regulations (CFR) title number and the specific section number (e.g., APHIS, 9 CFR 113.102): none.

Agency CFR

This report is required by law (7 USC 2143). Failure to report according to the regulations can See reverse side for result in an order to cease and desist and to be subject to penalties as provided for in Section 2150. additional information,

Interagency Report Control No I 01 80-DOA-AN


I

3. REPORTING FACILITY (List all locations where animals were housed or used in actual research. testing, teaching. or experimentation, or held for these purposes. Attach additional hnnts if necesaawb I

ANNUAL REPORT OF RESEARCH FACILITY ( W E OR PRINT)

FACILITY LOCATlONS(s#es)

FLORIDA A&M UNIVERSITY TALLAHASSEE, FL 32307-3800

1. REGISTRATION NO. CUSTOMER NO. 58-R-0012 874

I

2. HEADQUARTERS RESEARCH FACILITY (Name and Address, as registered w#h USDA, indude Z@ Code)

FAMU COLLEGE OF PHARMACY 201 DYSON PHARMACY BLDG TALLAHASSEE, FL 32307-3800

REPORT OF ANIMALS USED BY OR UNDER CONTROL OF RESEARCH FACILITY (Attach add#ional sheets if necessary or use APHIS FORM 7023A)



Welfare Regulations

B. Number of animals being bred. conditioned, or held for use in teaching. testing, experiments. research, or surgery but not yet used for such purposes.

4. Dogs

5. Cats --

7. Hamsters -

8. Rabbits

9. Non-Human Primates I I

C. Number of animals upon which teaching. research, experiments, or tests were conducted

1 1. Pigs

involving no and for which appropriate pain, distress, or anesthetic, analgesic, or use of pain- tranquilizing drugs were relieving drugs. I d.


D. Number of animals upon which experiments. teaching, research, surgery, or tests were conducted involving accompanying pain or distress to the animals

experiments, surgery, or tesk (An explanation of the procedures producing pain or distress in these animals and the masons such drugs were not used must be attached to this twodl

13. Other Animals

E. Number of animals upon which teaching, experiments, research, surgery or tests were conducted involving accompanying pain or distress to the animals and for which the use of appropriate anesthetic,analgesic. or tranquilizing drugs would have adversely affected the procedures, results, or intermetation of the teachina. research.


F.


(Cob. C + 0 + E)

1 ) Professionally acceptable standards governing the care, treatment, and use of animals, including appropriate use of anesthetic, analgesic, and tranquilizing drugs, prior to, during. and following actual research, teaching, testing, surgery, or experimentation were followed by this research facility.


3 ) This facility is adhering to the standards and regulations under the Act, and it has required that exceptions to the standards and regulations be specified and explained by the principal investigator and approved by the Institutional Animal Care and Use Committee (IACUC). A summsry of all the exceptlonr is attached to this amual report. In addition to identifying the IACUC-approved exceptions, this summary includes a brief explanation of the exceptions, as well as the species and number of animals affected.


I CERTIFICATION BY HEADQUARTERS RESEARCH FACILITY OFFICIAL 3 (Chief Executive m c e r or Legally Responsible Institutional official)

I cetii that the above is t w , correct, and complete (7 U.S.C. Section 2143) SIGNATURE OF C.E.O. OR INSTITUTIONAL OFFICIAL NAME & TITLE OF C.E.O. OR INSTITUTIONAL OFFICIAL (Type or Print) DATE SIGNED

I I APHIS FORM 7023 (Replaces VS FORM 18-23 (Oct 88), whkh b obsolete

(AUG 91 ) PART 1 - HEADQUARTERS

rr .re<' I 5 req.reao, an 7 .SC?l43) Fa ~rslo'eponnccor3rqloirereg. a'onrcan See allached form lor :rWspency Reron CcnlroNo .I ,, an croer IO ced~eara oer n aro lo oc i.c.ec< to penalter ar pro" a m lor n Sect on 21 ~ ! V ~ B I n l m r n ~ ~ ~

A - U N ~ E O STATES DEPARTMENT OF AGRICULTURE

ANIMAL AND PLANT HEALTH INSPECTION SERVICE I 1. CERTIFICATE NUMBER: 58-R-0015 I FORM APPROVED OM0 NO. 0579-0036

CUSTOWR NUMBER: 930 1 ANNUAL REPORT OF RESEARCH FACILITY

( T Y P E OR PRINT )

University Of South Florida 12901 Bruce 0. Downs Blvd Mdc 20 Tampa, FL 33612

I Telephone: (813)-- 979-6743 I I I J 1. REPORTING FACILITY ( Lid eii localions where animals were hweed or used in adud research, Issling. cx exwimsnlalion, a held fw these purpmsr. Atlach addiiional she& 1 necsrrary )

FACILITY LOCATIONS ( Sites ) - Sea Atached Listing

REPORT OF ANIMALS USED BY OR UNDER

Antmdr Covered BY he Animal

Welfare Reg~ Ia l l~ns

6. Number D f animal bemg bnd. condillonad. or held lor in teaching, ieollng. expaiments. research. 01 surgery but no1 fl used for such purporsr.

4. Dcgs

5. Cats

6. Guinea Pigs

7. Hamsters - 8. Rabbits


10. Sheep

11. Pigs


Goats

13. Other Animals

F e r r e t s

4TROL O F RESEARCH FACILITY 1 Attach addlt lonal sheets If nscssrarv or use APHIS Form 702U I I

2) Each principal invesligatw has considered allmativsr 10 painhtl procedures

, ~~~ - ~ . lesls yore w i~~emrstaon dthe lseciwng, r&h, expenmmn.

3, Thts IOC l y s aonsnng tome rlandaror ana replal,onr mdsr me An. m a $1 nssreq~lreo lnat sxcepllonr lo !ha SlanaamP m a rq.lstmr Dsipechea and erg alnea oy me gnncpal nrerngata ,no ap I~i!!.!.ona A r r r o r e an0 use Commnee . A C L A w m m y ot all such.xcsptbm hatuchsd tolhm annual r e p m m aaolon lo osn!.h/mq ine I A C L C - ~ P D ~ O ~ ~ . r r c * c r s :ns r.rmsr, n rnPl erc a m on CI me exremonr ar r e as msspeces and nrmoa of m m a r ancrtea

I Tne attenong .pav 31 anlot th J reresrcn lnr I ly nea apcmpnala a.lnont, losnsrre me~rmdon dadcq-ate wtennary raream lo we-ms aomvacy d omer i r ~ e c ' r 3 an mso care ana >re

I CERTIFICATION BY HEADQUARTERS RESEARCH FAClL lN OFFICIAL ( Chef Exec,twe Oifcer or Legally Respamoo s tnsl!l~lonal OI(;c,al )

cmd~ned invdving no pain. dlsVBU. OT use D

pain-relieving dwg..

r Pnol j

APHISFORM7023 hlch is Obrdele I

distress 10 the animals an lawhich appmpriae ansrlhelis. analgastc. a mnqllllidng dwgt wem usad.

WWY. or t e e . ( *n explanation d lha pmedures prcduung pain or dislreu in lhaaa animals and the reas, such dwgs were no( used must be anached lo this rapon

( COLUMNS c + D + E )




7 2. Number of animals used in this study

3. Species (common name) Nonhuman primatesf animals used in the study.


These animals are pre-diabetic models wich predisposes :hem to a high likelihood of the development of cardiovascular conditions, such as atherosclerosis, and other co-morbidities, such as neuropathy, blindness, dermatitis, and urinary tract infections, which have the potential to produce pain, discomfort, or distress.

5. Provide scientific justification why pain and/or distress could not be relieved. State methods or means used to determine that pain andlor distress relief would interfere with test results. (For Federally mandated testing, see Item 6 below)

The natural aging process and development of diabetic-related conditions and associated pathologies can potentially produce pain, discomfort, or distress. Veterinarians are always consulted when pain, discomfort, or distress is recognized. All pathological processes associated with thb agin and pre-diabetic condition are evaluated and those that are amenable to current veterinary medical care standards are promptly placed on appropriate pharmacological therapies.


Agency CFR


This form is intended as an aid to completing the Column E explanation. It is not an official form and its use is voluntary. Names, addresses, protocols, veterinary care programs, and the like. are not required as part of an explanation. A Column E explanation must be written so as to be understood by lay persons as well as scientists.


2. Number 8 of animals used in this study.

3. Species (common name) Rabbits of animals used in the study.


Antibody production may produce local inflammation in animals administered emulsions containing Complete Freunds' Adjuvant and can potentially produce pain, discomfort, or distress.

Veterinarians are always consulted when pain, discomfort, or distress is recognized a d analgesics prescribed where indicated.

5. Provide scientific justification why pain andlor distress could not be relieved. Slate methods or means used to determine that pain andlor distress relief would Interfere with test results. (For Federally mandated testing, see Item 6 below)

Antibody production can potentially produce ~a in , discomfort, or distress associated with the development of sterile granuloma(s) at the immunization sites. This cannot be reliably anticipated. Pain induced by these processes is treated in accordance with current veterinary medical care standards; i.e. localhystemic treatment; analgesics.


Agency CFR

T Interagency Report Control No.: This report is required by law (7 USC 2143). Failure to report according to the regulations can N 0 v 2 2 See attached form for

result in an order to cease and desist and to be subject to penalties as provided for in Section 21! additional information.


1. CERTIFICATE NUMBER: 58-R-0016 I FORM APPROVED OMB NO. 0579-0036 I

CUSTOMER NUMBER: 875 I I University Of Central Florida 12443 Research Parkway Suite 207 Orlando, FL 32826


Telephone: (407) -823-3778

I

,. REPORTING FACILITY ( List all locattons where animals were housed or used in actual research, testing, or experimentation, or held for these purposes. Attach additional sheets if necessary ) 3 FACILITY LOCATIONS ( Sites ) - See Atached Listing

REPORT OF ANIMALS USED BY OR UNDER CONTROL OF RESEARCH FACILITY I Attach additlonal sheets if necessarv or use APHIS Form 7023A 1 I

C. Numberof animals upon which teaching, research, experiments, or tests were conducted involving no pain, distress, or use a pain-relieving drugs.

0. Number of animals upon which experiments. teaching, research, surgery, or tests were conducted involving accompanying pain or distress to the animals an for which appropriate anesthetic, analgesic, or tranquilizing drugs were used.

E. Number of animals upon which teaching, experiments, research, surgery or tests were conducted involving accompanying pain or distress to the animals and for wh the use of appropriate anesthetic, analgesic, or tranquiliz drugs would have adversely affected the procedures, res or interpretation of the teaching, research, experiments. surgery, or tests. ( An explanation of the procedures producing pain or distress in these animals and the reast such drugs were not used must be attached to this reporl

B. Number of animal being bred. conditioned, or held for use in teaching, testing. experiments.




research, or surgery but not ye used for such purposes. I

6. Guinea Pigs I 0 I

4. Dogs

5. Cats

7. Hamsters I

0 0

8. Rabbits I O I 9. Nonhuman Primates I 0 I 10. Sheep

11. Pigs

I ASSURANCE STATEMENTS

0

0


13. Other Animals

1) Professionally acceptable standards governing the care, treatment, and use of animals, including appropriate use of anestetic, analgesic, and tranquilizing drugs, prior to, during, and following actual resc teaching, testing, surgery, or experimentation were followed by this research facility.

0

0


3) This facility is adhering to the standards and regulations under the Act, and it has required that exceptions to the standards and regulations be specitied and explained by the principal investigator and ap lnstitutional Animal Care and Use Committee (IACUC). A summary of all such exceptions is attached to this annual report. In addition to identifying the IACUC-approved exceptions, this summary in< brief explanation of the exceptions, as well as the speaes and number of animals affected.



S FORM 18-23 (OCT 88). which is obsolete.)

This report is required by law (7 USC 2143) Failure to report according to the regulations Can NO V 3 0 200# ee attached form for result in an order to cease and desist and to be subiect to ~enalties as arovided for in Section 21! dditional information.

Interagency -0.:



I. CERTIFICATE NUMBER: 58-R-0020

CUSTOMER NUMBER: 82 1 6

Florida Institute Of Technology 150 W. University Blvd. Melbourne, FL 32901

FORM APPROVED OMB NO. 0579-0036

I. REPORTING FACILITY ( List all locations where animals were housed or used in actual research, testing, or experimentation, or held for these purposes. Attach additional sheets if necessary ) i FACILITY LOCATIONS ( Sites ) - See Atached Listing

I purposes. I

REPORT OF ANIMALS USED BY OR UNDER CONTROL OF RESEARCH FACILITY I Attach additional sheets if necessarv or use APHIS Form 7023A \

,6. Guinea Pigs

7. Hamsters

A.


Welfare Regulations

I 1. Pigs

12. Other Farm Animals I I

B. Number of animal being bred, conditioned, or held for use in teaching, testing, experiments, research, or surgery but not yr used for such

13. Other Animals 0

I


C. Number of animals upon which teaching, research, experiments, or tests were conducted involving no pain, distress, or use o pain-relieving drugs.

D. Number of animals upon which experiments, teaching, research, surgery, or tests were conducted involving accompanying pain or distress to the anlrnals an for which appropriate anesthetic, analgesic, or tranquilizing drugs were

E. Number of animals upon which teaching, experiments. research, surgery or tests were conducted involving accompanying pain or distress to the animals and for wh the use of appropriate anesthetic, analgesic, or tranquiliz drugs would have adversely affected the procedures, res or interpretation of the teaching, research, experiments. surgery, w tests. ( An explanation of the procedures producing pain or distress in these animals and the reasc such drugs were not used must be attached to this report



used.

reportable to in the &t I

year. We have used only mice, rats, snakes and f ish ,

1) Professionally acceptable standards governing the care, treatment, and use of animals, including appropriate use of anestetic, analgesic. and tranquilizing drugs, prior to, during, and following actual resf teaching, testing, surgery, or experimentation were followed by this research facility.


3) This facility is adhering to the standards and regulations under the Act, and it has required that exceptions to the standards and regulations be spedfied and explained by the principal investigator and ap Institutional Animal Care and Use Committee (IACUC). A summary of all such exceptions is attached to this annual report. In addition to identifying the IACUC-approved exceptions, this summary inc brief explanation of the exceptions, as well as the species and number of animals affected.



/ I 1 DATE SIGNED 1

rs VS FORM 18-23 (OCT 88). which is obsolete.)

~ h , s rmon ,r required by law (7 USC 2143). Failure to repan according to the regulations can See ~ W O M slda for Interagency Repat Control No resu' .I an order tocease and derislandto be wbpd 10 pmaltim ar pmvided for in Seclion 2150. addllional information. 0180-WA-AN

UNITED STATES DEPARTMENT OF AGRICULTURE I. REGISTRATION NO. CUSTOMER NO. ANIMAL AND PLANT HEALTH INSPECTION SERVICE 58-R0021 868 FORMAPPROVED

OM0 NO. 05794036 I

I 3. REPORTING FACILITY [LISI a11 local,answhere animals were housdor used in actual rerearch, lasting. leaching, aerpsnrnentat~on, or held for thaw ppumorer. Anach additional

rheelr ,f necessary ) FACILITY LOCATIONS(OIBS~

MANNHEIMER FOUNDATION. INC. HOMESTEAD, FL 33034

MANNHEIMER FOUNDATION INC HOMESTEAD, FL 33034


4. Dogs

5. Cats

6. Guinea Pigs

7. Hamsters

2. HEADQUARTERS RESEARCH FACILm (Name and Addrsss, as regisfered wIh USDA. bauds z@ code)

THE MANNHEiMER FOUNDATION INC 20255 SW 360 ST. HOMESTEAD. FL 33034

8. Rabbits

9. Non-Human Primates

10. Sheep

11. Pigs

13. Mher Animals

12. Mher Farm Animals

2) Each principal lnvesligalar has conridered aitemsliva to painful pmcsdurer

820

I

4) The snendlng vetmnanan far lhl9 researdl fas8llW has appmpnate aulhcdiy lo ensure me pmvislm d adequate velerinanl ram and to o m the Bde6uacy of oth~r aspens d anmai cars and use

I CERTIFICATION BY HEADQUARTERS RESEARCH FACILITY OFFICIAL (Chief Executive Officer or Legallv Rer~onsible lnstltutional otlicial) I

I I I

. i ~ l r t l f y ma1 ms above is Ina, m&:and &mplstst7 U S C Secllon 2143)

.

SIGNATURE OF C.E.O. OR INSTITUTIONAL OFFICIAL NAME A T I N E OF C.E.O. OR INSTINTIONAL OFFICIAL F y o e or Pnm) DATE SIGNED

220

1 11/09/2004 I I I I I APHIS FORM 7023 (Replac.. VS FORM 18-23 (OM a), whkh h Ob.ol.(. PART 1 -HEADQUARTERS

17

(AUG 91)

237

-

APHIS Form 7023 Column E Explanatlon

This form is intended as an aid to completing the APHIS Form 7023 Column E explanation. It is not an official form and its use is voluntary. Names, addresses, protocols, veterinaly care programs, and the like, are not required as part of an explanation. A Column E explanation must be written so as to be understood by lay persons as well as scientists


213. Species (common name) 8 Number of animals used in this study:

Non-Human Primates (17)

4. Explain the procedure producing pain and/or distress.

The (17) Non-Human Primates have been physically restrained and an orogastric or nasogastric tube placed in order to be medicated. These two procedures do not cause pain, so pain management is not indicated.

5. Provide scientific justification why pain andlor distress could not be relieved. State methods or means used to determine that pain and/or distress relief would interfere with test results. (For Federally mandated testing, see Item 6 below)

Induction of chemical restraint with Ketamine or similar class of drugs. may interfere with the distribution or pharmacok~netics of the compounds used in these pharmacology studies.

6. What, if any, federal regulations require this procedure? Cite the agency, the code of Federal Regulations (CFR) title number and the specific section number (e.g.. APHIS. 9 CFR 113.102):

Agency: N/A CFR:

See attached form for additional information.

Interagency Report Control 0.: 4 This report is required by law (7 USC 2143). Failure to report according to the regulations can ,result in an order to cease and desist and to be subiect to penalties as Provided for in Section 21!

- -- -


1. CERTIFICATE NUMBER: 58-R-0023 I FORM APPROVED OMB NO. 0579-0036

CUSTOMER NUMBER: 9 14

St. Petersburg Junior College 7200 66th St ~ o r t h OCf 1 8 a111 ANNUAL REPORT OF RESEARCH FACILITY

( TYPE OR PRINT ) - - -

Pinellas Park, FL 33781

Telephone: (727) -341 -3652

I 3. REPORTING FAClLrrY ( Ust all locations where animals were housed or used in actual research, testing , or experimentation, or held for these purposes. Attach additional sheets if necessary ) I

FACILITY LOCATIONS ( Sites ) - See Atached Listing

1 REPORT OF ANIMALS USED BY OR UNDER CONTROL OF RESEARC

B. Number of animal being bred, conditioned, or

H FACILITY I Attach additional sheets If necessaw or use APHIS Form 7023A 1

Number of animals upon which teaching, research. experiments, or tests were conducted involving no pain, distress, or use a pain-relieving drugs.

D. Number of animals upon which experiments. teaching, research, surgery, or tests were conducted involving accompanying pain or distress to the animals an for which appropriate anesthetic, analgesic, or tranquilizing drugs w e used.


E. Number of animals upon which teaching, experiments, research, surgery or tests were conducted involving accompanying pain or distress to the animals and for wfi the use of appropriate anesthetic, analgesic, or tranquiliz drugs would have adversely affected the procedures, res or interpretation of the teaching, research, experiments, surgery, or tests. ( An explanation of the procedures producing pain or distress in these animals and the reasc such drugs were not used must be attached to this repod


Welfare Regulations

held for use in teaching, testing, experiments, research, or surgery but not ye used for such


4. Dogs

5. Cats

6. Guinea Pigs

7. Hamsters

8. Rabbits

9. Non-hurnan Rimates ( I 10, Sheep

12. Other Farm Animals HO r s e s I Cattle

13. Other Animals I I


1) Professionally acceptable standards governing the care, treatment, and use of animals, induding appropriate use of anestetic, analgesic, and tranquilizing drugs, prior to, during, and following actual resc teaching, testing, surgery, or experimentation were followed by this research facility.

2) Each principal investigator has considered alternatives to painful procedures. . .

3) This facility is adhering io the standards and regulations under the Act, and it has required that exceptions to the standards and regulations be specified and explained by the principal investigator and aP Institutional Animal Care and Use Committee (IACUC). A summary of all such exceptions & attached to tMs annual report. In addition to identifying the IACUC-approved exceptions, this summary inc brief explanation of the exceptions, as well as the species and number of animals affected.


ERTlFlCATlON BY HEADQUARTERS RESEARCH FACILITY OFFICIAL

58-R-0035 J

ANIMAL AND PLANT HEALTH INSPECTION SERVICE FORM APPROVED OMB No. 0579-8036

CUSTOMER NUMBER: 908 I

ANNUAL REPORT OF RESEARCH FACILITY (TYPE OR PRINT) I Florida Atlantic University

777 Glades Road P.O. Box 3091 Boca Raton, FL 3343 1

I I Telephone: (561) -367-23 10 I I

3. REPORTING FACILITY (List aU locations where animals were housed or used in actual research, testing, teachug, or experimentation, or held for these purposes. Attach additional sheets if necessary) I FACILITY LOCATIONS (Sites) - See Attached Listing

REPORT OF ANIMALS USED BY OR UNDER CONTROL OF RESEARCH FACLllTY (Attach additional sheets if fiecessarv or use APHIS FORM 7023A.J

Animals Covered 9. The Animal

Welfare Reguhtioos

4. Dons

B. Number of animals being b d , conditioned, or held for use in teacbiIq4, testin& experiments, reatarch, or surgery but w t yet Psed for such pu'P-=

0

5. Cats

6. Guinea Pig 0

7. Hamsters 0

8. Rabbits 0


10. Sheep 0

I I. Pigs 0

12. Other Farm Animals 0

13. Other Animals I 0

C. Number of animals upom which teaching, -=8, experiments, or tests wen conducted involving a0 pain, distress, or use of pails- relieving drugs.

0

TOTAL NUMBEF OF ANIMAL

b. Number of animals upon which experiments, teaching, research, surgery, or tests were conducted involving acmmpanying pain e r distress to the animals and for which appropriate anesthetic, analgesic, or tranquilizing drugs wen used.

(COLUMNS C+D+E)

E. Number of animals upom which teaching, experimenls, research, surgery or tests were conducted involving accompanying pain or distress to the animah and for which the use of appropriate anesthetic, analgesic, or tranquilizing drugs would have advemdy affected tire procedures, results, or interpretation of the leaching, reatarch, experiments, surgery, or tests. (An explanation of the procedures producing pain or distress in these animals and the reasons such drags were not IUUI must be attached fo this report).


1) Professionally acceptable standards governing the care, treatment, and use of animals. including appropriate use of anesthetic, analgesic, and tmmphng he, prim to, durm& and following actual researcb teaching, testiag, surgery, or experimentation were followed by this research h&y.

2) Each principal investigator has considered alternatives to p a d 3 procedures.

3) This facility is adhering to the standards and regulations under the Act, and it has required that exceptions to the standards arid regulations be specified and explained by the principal investigator arid approved by the Institutional Animal Care arid Use Committee (IACUC). A summary of aU such exceptions is attached to this annual report In addition to ldenhfilmg the IACUC-approved exceptions, this summary inchdes a brief explanation of the exceptions, as well as the species and number of ammals affected

4) The attending veterinarian firr this research facility has appropriate authority to ensure the provision of adequate veterinary care and to oversee the adequacy of other aspects of animal care arid use.

CERTIFICATION BY HEADQUARTERS RESEARCH FACILITY OFFICIAL 1 (Chief Executive Offwer or Legally Responsible Institutional Official)

Interagency Report Contr Y This report is required by law (7 USC 2143). Failure to report according to the regulations can result in an order to cease and desist and to be subject to penalties as provided for in Section 21!


UNITED STATES DEPARTMENT OF AGRICULTURE ANIMAL AND PLANT HEALTH INSPECTION SERVICE I

-- -- --

1. CERTIFICATE NUMBER: 58-~-0037 I FORM APPROVED OMB NO. 0579-0036

CUSTOMER NUMBER: 82 1 8

Rumbaugh-Goodwin lnstitute For Cancer Research Goodwin Institute For Cancer Research 1850 N.W. 69th Avenue Plantation, FL 3331 3


I 3. REPORTING FACILITY ( List all locations where animals were housed or used in actual research, testing, or experimentation, or held for these purposes. Attach additional sheets if necessary )


REPORT OF ANIMALS USED BY OR UNDER CONTROL OF RESEARCH FACILITY I Attach additional sheets if necessaw or use APHIS Form 7023A 1

B. Number of animal being bred, conditioned, or held for use in teaching, testing, experiments, research, or surgery but not ye used for such purposes.

-

c. Number of animals upon wh~ch teaching, research, experiments, or tests were conducted involving no pain. distress, or use a pain-relieving drugs.

- - - -

D. Number of animals upon which experiments. teaching, research. surgery, or tests were conducted involving accompanying pain or distress to the animals an for which appropriate anesthetic, analgesic, or tranquilizing drugs were used.

E. Number of animals upon which teaching, experiments, research, surgery or tests were conducted involving accompanying pain or distress to the animals and for wh the use of appropriate anesthetic, analgesic, or tranquiliz drugs would have adversely affected the procedures, res or interpretation of the teaching, research, experiments, surgery, or tests. ( An explanation of the procedures producing pain or distress in these animals and the reasc such drugs were not used must be attached to this reporl


Welfare Regulations



4. Dogs I 5. Cats I 6. Guinea Pigs I 7. Hamsters I 8. Rabbits I

10. Sheep


13. Other Animals


1) Professionally acceptable standards governing the care, treatment, and use of animals, including appropriate use of anestetic, analgesic, and tranquilizing drugs, prior to, during, and following actual resc teaching, testing, surgery, or experimentation were followed by this research facility.


3) This facility is adhering to the standards and regulations under the Act, and it has required that exceptions to the standards and regulations be specified and explained by the principal investigator and ap Institutional An~mal Care and Use Committee (IACUC). A summary of all such exceptions is attached to this annual report. In addition to identifying the IACUC-approved exceptions, this summary in( brief explanation of the exceptions, as well as the species and number of animals affected.



1 DATE SIGNED

( AUG 91 )

~ j - / / * U L /

APHIS FORM 7023 (Replaces VS FORM 18-23 (OCT 88). which is obsolete.) I

Interagency Report Control No. eb' This report IS required by law (7 USC 2143). Fanlure to report according to the regulations can N 0 Y 1 5 2001 See anached form for result ~n an order to cease and desist and to be subject to penalties as provided for in Section 21! additional tnforrnation.

1. CERTIFICATE NUMBER: 58-R-0045



8 FORM APPROVED OMB NO. 0579-0036

- Dolphin Research Center 58901 Overseas Hwy Grassy Key, FL 33050


Telephone: (305) -289-0002

I I

3. REPORTING FACILITY ( List all locations where animals were housed or used in actual research, testing 1, or experimentation, or held for these purposes. Attach additional sheets if necessary ) I FACILITY LOCATIONS ( Sites ) - See Atached Listing

REPORT OF ANIMALS USED BY OR UNDER CONTROL OF RESEARCH FACILITY I Attach additional sheets if necessaw or use APHIS Form 7023A 1 1 A. B. Number of animal 1

being bred, conditioned, or

Animals Covered held for use in By The Animal teaching, testing,

Welfare Regulations experiments, research, or surgery but not ye used for such

C. Number of animals upon which teaching. research, experiments. or tests were conducted involving no pain, distress, or use Q

pain-relieving drugs.

D. Number of animals upon which experiments, teaching, research, surgery, or tests were conducted involving accompanying pain or distress to the animals an for which appropriate anesthetic, analgesic, or tranquilizing drugs were used.

E. Number of animals upon which teaching, experiments, research, surgery or tests were conducted involving accompanying pain or distress to the animals and for wh the use of appropriate anesthetic, analgesic, or tranquiliz drugs would have adversely affected the procedures, res or interpretation of the teaching, research, experiments, surgery, or tests. ( An explanation of the procedures producing pain or distress in these animals and the reasc such drugs were not used must be attached to this report



purposes.

4. Dogs

5. C.ats

6. Guinea Pigs

7. i-(amsters

8. Rabbits


10. Sheep

11. Pigs


13. Other Animals I I

1 ASSURANCE STATEMENTS - - - - - -- -- --

I) Professionally acceptable standards governing the care, treatment, and use of animals, including appropnate use of anestetic, analgesic, and tranquilizing drugs. prior to, during, and following actual rest teachmg, testing, surgery, or experimentation were followed by this research facility.


3) This facility is adhering to the standards and regulations under the Act, and it has required that exceptions to the standards and regulations be specified and explained by the principal investigator and ap Institutional Animal Care and Use Committee (IACUC). A summary of all such exceptions is attached to this annual report. In addition to identifying the IACUC-approved exceptions. this summary in( brief explanation of the except~ons. as well as the species and number of an~mals affected.

4) The attending veterinarian for this research facility has appropriate authority to ensure the provision of adequate veterinary care and to oversee the adequacy of other aspects of animal care and use


SIGNATURE OF C.E.O. OR INSTITUTIONAL OFFICIAL I NAME 8 TITLE OF C.E.O. OR INSTITUTIONAL OFFICIAL (_Type or Print) I DATE SIGNED

This report is requ~red by law (7 USC 2143). Failure to report according to the regulations can O " i ?OD' See attached form for Interagency Report Control No.:

result in an order to cease and desist and to be subject to penalties as provided for in Section 21! additional information.


Bild Animal Hospital 2500 Nw 79th St. Miami, FL 33147

1. CERTIFICATE NUMBER: 58-~-0058




I I 3. REPORTING FACILITY ( List all locations where animals were housed or used in actual research, testing, or experimentation. or held for these purposes. Attach additional sheets if necessary ) 1


REPORT OF ANIMALS USED BY OR UNDER CONTROL OF RESEARCH FACILITY I Attach additional sheets if necessarv or use APHIS Form 7023A 1 I I

A. B. Numberof animal 4 being bred, conditioned, or

Animals Covered held for use in By The Animal teaching, testing.

Welfare Regulations experiments, research, or surgery but not ye used for such


Number of anlmals upon which experiments. teaching, research, surgery, or tests were conducted involving accompanying pain or distress to the animals an for which appropriate anesthetic, analgesic, or tranquilizing drugs were used.




4. Dogs 0 I 5. Cats I n

6. Guinea Pigs I I) I 7. Hamsters

8. Rabbits 0

9. Non-human Primates -I--d 10. Sheep

11. Pigs

13. Other Animals I 12. Other Farm Animals


I 1 ) Professionally acceptable standards governing the care, treatment, and use of animals, including appropriate use of anestetic. analgesic. and tranquilizing drugs, prior to, during, and following actual res6

teach~ng, testing, surgery, or experimentation were followed by this research facility.

0


3) This facility is adhering to the standards and regulations under the Act, and it has required that exceptions to the standards and regulations be specified and explained by the principal investigator and ap Institutional Animal Care and Use Committee (IACUC). A summary of all such exceptions is attached to this annual report. In addition to identifying the IACUC-approved exceptions, this summary inc br~ef explanation of the exceptions, as well as the species and number of an~mals affected.


CERTIFICATION BY HEADQUARTERS RESEARCH FACILITY OFFICIAL

DATE SIGNED

APHIS FORM 7023 ( R p p l a c e s ~ ~ FORM 18-23 (OCT 881, which is obsolete.)

( AUG 91 ) t/

Interagency R e p m k o 01 80-DOA-AN

This report is required by law (7 USC 2143). Failure to report according to the regulations can result in an order to cease and desist and to be subject to penalties as provided for in Section 2150.

See reverse side for additional information. - .

UNITED STATES DEPARTMENT OF AGRICULTURE I. REGISTRATION NO. CUSTOMER NO. ANIMAL AND PLANT HEALTH INSPECTION SERVICE 58-R-0103 8214 FORM APPROVED

OM8 NO. 0579-0036

ANNUAL REPORT OF RESEARCH FACILITY 2. HEADQUARTERS RESEARCH FACILITY (Name and Address, as regatered with USDA,

include Zip Code)

(TYPE OR PRINT) SCHOOL OF NAT & HEALTH SCIENCE 11300 NE SECOND AVE MIAMI SHORES, FL 33161 (305) 899-3200

3. REPORTING FACILITY (List all locations where animals were housed or used in actual research, testing, teaching, or experimentation, or held for these purposes. Attach additional sheets if necessaw.)

FACILITY LOCATlONS(sites)

I REPORT OF ANIMALS USED BY OR UNDER CONTROL~F RESEARCH FACILITY (Attach additional sheets if necessary or use APHIS FORM 7023A )

0. Number of animals upon I E. Number of animals upon which teaching, I F. 8. Number of animals being bred, conditioned, or held for use in teaching, testing, experiments, research, or surgery but not yet used for such purposes.

C. Number of animals upon which teaching, research. experiments, or tests were conducted involving no pain, distress, or use of pain- relieving drugs.

which experiments, teaching, research. surgery, or tests were conducted involving accompanying pain or distress to the animals and for which appropriate anesthetic, analgesic, or tranquilizing drugs were used.

-- experiments, research. surgery or tests were conducted involving accompanying pain or distress to the animals and for which the use of appropriate anesthetic,analgesic, or tranquilizing drugs would have adversely affected the procedures, results, or interpretation of the teaching, research, experiments, surgery, or tests. (An explanation of the procedures producing pain or disfress in these animals and the reasons such drugs were not used must be attached to this report)



Welfare Regulations (Cols. C +

D + E)

4. Dogs --

5. Cats

6. Guinea Pigs 1 A

7. Hamsters I A

8. Rabbits d 9. Non-Human Primates

10. Sheep I

1 I. Pigs


13. Other Animals


1) Professionally acceptable standards governing the care, treatment, and use of animals, including appropriate use of anesthetic, analgesic. and tranquilizing drugs, prior to, during, and following actual research, teaching, testing, surgery, or experimentation were followed by this research facility.


3) This facility is adhering to the standards and regulations under the Act, and it has required that exceptions to the standards and regulations be specifled and explained by the principal investigator and approved by the Institutional Animal Care and Use Committee (IACUC). A summary of all the exceptions is attached to this annual report. In addition to identifying the IACUC-approved exceptions, this summary indudes a brief explanation of the exceptions, as well as the species and number of animals affected.


CERTIFICATION BY HEADQUARTERS RESEARCH FACILITY OFFICIAL (Chief Executive Officer or Legally Responsible Institutional official)

I c e w that the above is true, correct, and complete (7 U.S.C. Section 2143) DATE SlGNED

HEADQUARTERS

Miami Children's Hospital George E Batchelor Research And Academic Pavilion 3196 Sw 62nd Ave Miami, FL 33155

'Thi8repMIsw.Jimdbylaw(7USCZ(U). h U V . D ~ e w a r d i n g t o ~ r e g u l - C M NOV 3 0 2001 ~ 8 l l r ( * 6 f o m f u - kdomuwn. RePad Conw No.: - reU in an wder to cease and d d s t and to be ~I~OCI to psnrl6m a provided for In Sedbn 21!

ANNUAL REPORT OF RESEARCH FACILITY ( TYPE OR PRIM )

UNITED STATES DEPAKfMENT OF AGRK=ULTURE ANIMAL AND PLANT HEALTH INSPECTION SERVICE

I I Telephone: (305) -663-8596

I

3. REPORTING FACILITY ( USI dl locations where mimais were homed or uaed in odual maeorch. tedng, or expwimmbtion, or hdd for these pwpms. A#eeh &JMUKII w if )

1. CERTIFICATE MIYbUI: ~ ~ - 0 1 09 CWOM€Rwu#eR: 850

I REPORT OF ANIMALS USED BY OR UNDER CONTROL OF RESW

FORM APPI#)VED OMB NO. ~790038

B. Number of a n i d being bred, conditioned, or held for use in teaching, tasting, exwlments, research, or surgery but not r U8ed f0r Such vP-=.

C. kmtmrol *upon wNch teaching, -, lsmd-, or testawere cmdudd invdving no pain. ~ a u s e u p.kwdlevrng -.

D. Numbefdmkndsugon which expwhents, leeching, -, =wY,or-- conduded invoMng ==mmngprlnw dlatm8~ to the Mknela an for whtch approplate enoatharc. rr#lWc. or barnl idng dwoa - d.

E. Wnbm d animda upon which (l#chlng, eqmimenta, mmuch, surgery or test8 wem conducted invdvlng a&mpmving pdn or dislmss to the aniW and for wh Um use d qpmprbk a m a t h d ~ mdgeslc, or traquillz d ~ w o u l d ~ r d v c K w l y r l l b d e d t h e ~ , res o r ~ d t h e ~ n g , ~ , e x p e r i m a n t a ,

F* . . - TOTAL NUMBER

OF ANIMALS

( COLUMNS

4. Dogs - --

5. Cats I 6. Guinea Pigs

7. Hamsters -- 8. Rabbits I

10. Sheep

11. Pigs I 12. Other Farm Animals

13. Other Animals 1 ASSURANCE STATEMENTS

1) PmlWmally -table slenderds gwemlng the care, trsament. and use d anhala. including .pprapriats rws d m ~ d l c , cmdgedc, and tranquitldng dmga, prior to, during. and fdlawing actual r a ~ teaching. testing. surgery, or ewpsrlmentetion ware followed by this mearch fadlity.

2) Each principal investigator has c~sidersd alsmathras b p.krM pmcedwa.

3) This fadlHy Is adhering to the standards and regulations m t m the Ad. and it has required that excepUw to the standards and mguIaUons be apedfid and ewlained by Ihe prkrdpal aP lnstltutionai Animal Cam and Uae Committee (MCUC). A mmmuy of dl such mtuptlonr b attachd b thb wwwl report. h addtion to identifying the IACXMhpproMd ex@OM. * m r Y l f~ brief explanation of the exceptions. as well as Ihe apdes and number d animals M o d .

41 The 8Mendimr veterinarian for this research fdl i tv has m a t e trulh#itv to sn#rra the woukion d m w - -- a m m m r d anhd CZNe md W.


APHIS FOR t

Attachment 3. Reporting Facility

Miami Children's Hospital George E. Batchelor Research and Academic Pavilion 3 196 S. W. 62nd Avenue Miami, FL 33 155

I

This report is rearlud br 'qw ( Z USC 2143). Fallwe la wwt accudinp 10 the repulmtim On Sea atladwd fan lar result in an orde; lo ceek and dosmt and be subject lo penallles as &odd& for In Section 21!

1. CERTFICATE NUWBER: 58-Ra 1 18 FORM APPROVED OW NO. 05794038

CUSTOMER 976

UNITED STATES DEPARTMENT OF AGRICULTURE AN- AN0 PLANT HEALTH INSPECTION SERVICE

Toxin Technology, Inc. 7165 Curtiss Ave. Sarasota, FL 34231


Telephone: (941 ) -925-2032

FAClLlTY LOCATIONS ( Sit= ) - See UIchcd U M g

) REPORT OF ANIMALS USED BY OR UNMR CONTROL OF RESEARCH FACILITY ( Attach additional s h M W necessm or use MI3 Form 70- b

It

... .- --

- - - - - -

- - - - -

-

- -

- - - -.

- -

8. Flunberafmimsl being bred. condimed. a held for urn in teaching. IesHrg, axpeirntms. research. or surgery but not yc used for such

0. Numbwdrrhn01s up~n nhich ex@ehenb, tea&ng,msemh surgery, or tests were condudre invddng K.compmySn9 P m of dsl- lo h. rnimels an lorwmckrppap~ msthdic. a ~ & s i e , or tranquil(tin9 chrgs nntre used.

E. ~w d m i m ~ s upon r r ~ c h t-ng, expafmsna. rrcrswch, surgery or asts conauaed involving

poinordlsheaetothsmhaI611ndlarvh the use of apprepfirte neslhetk. maigesic. or tmnquliz dm@s would hrve sdvmdy rffsded me procedures. rw or iNCIpWtkm d the lerddng, ntwrch, rxpeflmmls, surgery, ar tests. ( An OxpkUlfan d the procedurar prarlucing psh w itstma In mirnals a d the rsr# such drugs wua not used mwl be anached to this rsporl

mAL NUMBER OF ANIWLS


11. Pigs I 12. Other Farm Animals

13. Other Animds ----.-I ASSURANCE STATEMENTS

1) Prokssian~lty a c c e m slenderds m i w the care. 1rea8nent. Lnd use of animals. hcludnp approprid. uu d mestatic. anolgmk. M V M q u i i i i drugs, prtar to. duflng. and fdlowring Wu.l teaching, tmiq. rvgery, crerrprimcnfstlan w e n fdldwed by lhis research fadlily.

2) Em9 @cipaI Inwfigrrlor has considemi alternatives to p a d d promhms.

3) This facltity Is adhering to the stRnderds end npMiom under Ihe Act. Md 11 has reajrsd lhel exceptions to lk slnckrds and regrdHions be spedlled and e@sined by lh. principal InvbSligmtor 8 nd ap hslilulionel Anima C#u? and Use Committee (IACUC). A suwwnary of all sudb ucoptlonz h al!.dwd (o thb amurl rrport. tn addlaon to identi* *e UCUGapprwccl rxepaions. thi3 summary inc brief explanation of the ~ p t ~ s , as wdl as h e species a d rn- d Stlimcrls dfeC&d.

4) The attending wmnafian far lhii mmrch facility hes appropriate artharlty k ensure the pmTOV& d adequate vsrwmaryws a d lb oversee tho dequncy dother aspets of animal cam and use.

CERTIFICATION BY HEADQUARTERS RESEARCH FACiLCrV OFFICIAL ( Chkf ExecuUue Officer or legally Reswmibb MMional OIlldel )

APHIS FORM 7023 (Replaces VS FORM 1B23 (OCT which is obsde(e.)


Interagency Report ~ontr& This report is required by law (7 USC 2143). Failure to report according to the regulations can result in an order to cease and desist and to be subject to penalties as provided for in Section 21!

UNITEO STATES DEPARTMENT OF AGRICULTURE ANIMAL AND PLANT HEALTH INSPECTION SERVICE

1. CERTIFICATE NUMBER: 5 8 - ~ - 0 1 20 CUSTOMER NUMBER: 1000 I FORM APPROVED

OMB NO. 0579-0036

Orhs Health Sciences Center Orhs Health Research Institute 110 Bonnie Loch Court Orlando, FL 32806

SEP 2 72004 ANNUAL REPORT OF RESEARCH FACILITY ( TYPE OR PRINT )

Telephone: (407) -426-9794

I, or experimentation, or held for these purposes. Attach additional sheets if necessary )


REPORT OF ANIMALS USED BY OR UNDER CONTROL OF RESEARC 1

H FACILITY I Attach additional sheets if necessarv or use APHIS Form 7023A

B. Number of animal being bred. conditioned. or held for use in teaching, testing, experiments, research, or surgery but not yc used for such purposes.

C. Number of animals upon which teaching. research, experiments, or tests were conducted involving no pain, distress. or use a pain-relieving drugs.

D. Number of animals upon which experiments, teaching, research. surgery, or tests were conducted involving accompanying pain w distress to the animals an for which appropriate anesthetic, analgesic, or tranquilizing drugs were used.





4. Dogs

5. Cats

6. Guinea Pigs

7. Hamsters

8. Rabbits


10. Sheep


13. Other Animals -----I-


1 ) Professionally acceptable standards governing the care, treatment, and use of animals, including appropriate use of anestetic, analgesic, and tranquilizing drugs, prior to, during, and following actual resr teaching, testing, surgery. or experimentation were followed by this research facility.


3) This facility is adhering to the standards and regulations under the Act, and it has required that exceptions to the standards and regulations be specified and explained by the principal investigator and ap Institutional Animal Care and Use Committee (IACUC). A summary of all such exceptions is attached to this annual report. In addition to identifymg the IACUC-approved exceptions, this summary in( brief explanation of the exceptions. as well as the species and number of animals affected.

4) The attendmu veterinarian for this research facility has appropriate authority to ensure the provision of adequate veterinary care and to oversee the adequacy of other aspects of animal care and use. . . . . J


S (DATE SIGNED

( AUG 91 )

APHIS FORM 7023 (Replaces VS FORM 18-23 (OCT 88), which IS obsolete.) 1 '

See attached form for 6

Interagency Report Control No.: additional information.

This report is required by law (7 USC 2143). Failure to report according to the regulations can result in an order to cease and desist and to be subject to penalties as provided for in Section 21!


1. CERTIFICATE NUMBER: 58-~-0 1 2 1 CUSTOMER NUMBER: 1663


Dumond Conservancy For Primates & T P 0 Box 246 ANNUAL REPORT OF RESEARCH FACILITY

( TYPE OR PRINT ) Miami, FL 33170

Telephone: (305) -238-9981

I 1, or experimentation, or held for these purposes. Attach additional sheets if necessary ) 3 I. REPORTING FACILITY ( List all locations where anlmals werehoused or used in actual research, tes

FACILITY LoCATloNs ( Sites ) - See Atached Llstlng '9755 / f a 5 3 bd 2 1 b 641- M I ~ I FL 33170 ~ Y * J AAZ:

1 REPORT OF ANIMALS USED BY OR UNDER ONTROL OF RESEARCH FACILITY I Attach additional sheets if necessaw or use APHIS Form 7023A 1 1 C. Number of

animals upon which teaching, research, experiments, or tests were conducted involving no pain, distress, or use a pain-relieving drugs.







4. Dogs I 5. Cats I 6. Guinea Pigs I 7. Hamsters I 8. Rabbits I

1 1. Pigs I 12. Other Farm Animals

13. Other Animals


1 ) Professionally acceptable standards governing the care, treatment, and use of animals, including appropriate use of anestetic, analgesic, and tranquilizing drugs, prior to, during, and following actual resc teachmg, testing, surgery, or experimentation were followed by this research facility.


3) This facllity is adhering to the standards and regulations under the Act, and it has required that exceptions to the standards and regulations be specified and explained by the principal investigator and ap lnstitutional Animal Care and Use Committee (IACUC). A summary of all such exceptions is attached to this annual report. In addition to identifymg the IACUC-approved exceptions, this summary inc brief explanation of the exceptions, as well as the species and number of animals affected.

4) The attending veterinarian for this research facility has appropriate authority to ensure the prov~sion of adequate veterinary care and to oversee the adequacy of other aspects of animal care and use.


( AUG 91 )

i ~

DATE SlGNEP

APHIS

resrAU in anordez to cease and des~st and to be subiect to penalties as ~rovided f i r in Section 21!

, This report is required by law (7 USC 2143). Failure to report according to the regulations can ov m1 Seeattachedform for

additional information. -- -- -


L


CUSTOMER NUMBER: 1796 - FORM APPRO&D OM0 NO. 0579-0036

Brevard Community College 151 9 Clearlake Road Cocoa, FL 32922

3. REPORTING FACILITY ( Llst all locations where animals were housed or used in actual research, tesl


Telephone: (321) -632-1 11 1 I

5

1. or experimentation, or held for these purposes. Attach additional sheets if necessary ) I FACILITY LOCATIONS ( Sites ) - See Atached Listing

B. Number of animal being bred, conditioned, or held for use in teaching, testing, experiments, research, or surgery but not yc used for such purposes.

1 REPORT OF ANIMALS

s. Number of animals upon which teaching, research, experiments, or tests were conducted

USED BY OR UNDER CONTROL OF RESEARCH FACILITY i Attach additional sheets if necessarv or use APHIS Form 7023A I I

D. Number of animals upon which experiments, teaching, research. surgery, or tests were conducted involving accompanying pain or distress to the animals an for which appropriate anesthetic, analgesic, or tranquilizing drugs were used.




Welfare Regulations ( COLUMNS C + D + E ) involving no pain,

distress, or use a pain-relieving drugs.

4. Dogs

6. Guinea Pigs

7. Hamsters

8. Rabbits


10. Sheep

11. Pigs

S i m a l s

13. Other Animals


I ) Professionally acceptable standards governing the care, treatment, and use of animals, including appropriate use of anestetic, analgesic. and tranquilizing drugs, prior to, during, and following actual resc teaching, testing, surgery, or experimentation were followed by this research facility.


3) This facility is adhering to the standards and regulations under the Act, and it has required that exceptions to the standards and regulations be specified and explained by the principal investigator and ap Institutional Animal Care and Use Committee (IACUC). A summary of all such exceptions is attached to this annual report. In addition to identifying the IACUC-approved exceptions, this summary in( brief explanation of the exceptions, as well as the species and number of animals affected.

4) The attending vetennarian for this research facility has appropriate authority to ensure the provision of adequate veterinary care and to oversee the adequacy of other aspects of animal care and use.


APHIS ~ 0 ~ ~ 7 0 2 3 (Replaces VS FORM 18-23 (OCT 88), which is obsolete )

DATE SIGNED

11-16.d 9

Addendum to APHIS Form #7023 2003104

Reporting facility (List all locations where animals were housed or used in research, testing, or experimentation, or held for these purposes*). *Animals at Brevard Community College are utilized for TEACHING purposes only at the following locations:

Brevard Community College: 0:. Cocoa Campus Veterinary Technology Building #2 1 Fine Arts Building #4 Various classrooms Institute for Community Education/BCC Lab School

0:. Titusville Campus Fine Arts Building - various classrooms Science Bldg

0% Melbourne Campus Criminal Justice Building #8

d

9 Palm Bay Science Bldg #1

Brevard County Sheriffs Ranch Alternative Sentencing Farm Cocoa, FL

Central Brevard Humane Society 1020 Cox Rd. Cocoa, FL

Cadavers and preserved specimens utilized for teaching purposes in the Veterinary Technology program @ Brevard Community College, Cocoa campus:

4. Preserved cadavers from Carolina Biological Supply: 5 canines 7 felines

9 Donated cadavers from the local humane society: 9 felines 3 canines

This repori IS required by law (7 USC 2143). Failure to report according to the regulations can result in an order to cease and desist and to be subiect to oenalties as orovided for in Section 21!


Interagency Report C trol No.: r - - - -

UNITED STATES DEPARTMENT OF AGRICULTURE ANIMAL AND PLANT HEALTH INSPECTION SERVICE I

-- -- -

1. CERTIFICATE NUMBER: 58-R-0 1 27 CUSTOMER NUMBER: 8985

FORMAPPROVED OMB NO. 0579-0036

Everglades Wildlife Sanctuary P 0 Box 2326 La Belle, FL 33935


I Telephone: (863) -64+M=W & ' 0 q 9 1 I I

3. REPORTING FACILITY ( List all locations where animals were housed or used in actual research, testing, or experimentation, or held for these purposes. Attach additional sheets if necessary ) 1 -- - - --


B. Number of animal being bred. conditioned, or held for use in teaching, testing, experiments. research, or surgery but not yc used for such purposes.

REPORT OF ANIMALS USED BY OR UNDER I

C. Number of animals upon which teaching. research, experiments, or tests were conducted involving no pain, distress, or use o pain-relieving drugs.

CONTROL OF RESEARCH FACILITY I Attach additional sheets if necessarv or use APHIS Form 7023A 1

0. Number of animals upon which experiments. teaching, research, surgery, or tests were conducted involving accompanying pain or distress to the animals an for which appropriate anesthetic, analgesic, or tranquilizing drugs were used.

E. Number of animals upon which teaching, experiments, research, surgery or tests were conducted involving accompanying pain or distress to the animals and for wh the use of appropriate anesthetic. analgesic, or tranquiliz drugs would have adversely affected the procedures, res or interpretation of the teaching, research, experiments, surgery, or tests. ( An explanation of the procedures producing pain or distress in these animals and the reasc such drugs were not used must be attached to this reporl




4. Dogs

5. Cats

6. Guinea Pigs

7. Hamsters

8. Rabbits


10. Sheep

11. Pigs


13. Other Animals I


1) Professionally acceptable standards governing the care, treatment, and use of animals, including appropriate use of anestetic, analgesic, and tranquilizing drugs, prior to, during, and following actual resr

i teaching, testing. surgery. or expenmentation were followed by this research facility.





DATE SIGNED


Interagency ~ e p o r t ? f & d J o . : This report is required by law (7 USC 2143). Failure to report according to the regulations can result in an order to cease and desist and to be subiect to ~enalties as orovided for in Section 21!

m

-

I. REPORTING FACILITY ( List all locations where animals were housed or used in actual research, testing, or experimentation, or held for these purposes. Attach additional sheets if necessary )

- - - -


-

1. CERTIFICATE NUMBER: 5843-01 28 I FORM APPROVED OM0 NO. 0579-0036


Lemur Conservation Foundation Po Box 249142500 73rd Ave E ANNUAL REPORT OF RESEARCH FACILITY

( TYPE OR PRINT ) Myakka City, FL 34251

Telephone: (941 ) -322-8494

REPORT OF ANIMALS USED BY OR UNDER CONTROL OF RESEARCH FACILITY I Attach additional sheets if necessarv or use APHIS Form 7023A 1 I 1 I I - - -

2. Number of animals upon whtch teachmg. research, expenments, or tests were conducted involwng no pain. distress, or use o pain-relieving drugs.


E. Number of animals upon which teaching, experiments, research, surgery or tests were conducted involving accompanying pain or distress to the animals and for wh the use of aopropriate anesthetic, analgesic, or tranquiliz drugs would have adversely affected the procedures, res or interpretation of the teaching, research, experiments. surgery, or tests. ( An explanation of the procedures producing pain or distress in these animals and the reasl such drugs were not used must be attached to this reporl

6. Number of animal being bred, conditioned, or held for use in teaching, testing. experiments, research, or surgery but not ye used for such purposes.




I

4. Dogs

5. Cats I 6. Guinea Pigs 7

I

7. Hamsters



13. Other Animals I

ASSURANCE STATEMENTS I 1) Professionally acceptable standards governing the care, treatment, and use of animals, including appropriate use of anestetic, analgesic, and tranquilizing drugs, prior to, during, and following actual resc





This report is required by law (? USC 21 43) Failure to report according to the regulations can Set reverse side for result In an order to cease and desist and to be subject lo penalties as provided for tn Section 2 150 additionalinformation

1 REGISTRATION NO. pp --- -


ANNUAL REPORT OF RESEARCH FACILITY ( W E OR PRINT)

I OM6 NO. 0579-0036

2. HEADQUARTERS RESEARCH FACILITY (Namr and Addreu, as registerad with USDA Include Zlp Codr)

- 3. REPORTING FACILITY (Lisl all kxatlons where anlmals were housed or used in actual research, testing. teaching. or experimentation, or held for these purposes. Attach additional

FACILITY LOCATIONS (S~tes)

REPORT OF ANIMALS USED BY

A

Animals Covered

By m e Anima Welfare Regulations

OR UNDER CONTROL OF RESEARCH FACILI

6. Number of C. Number of animals being animals upon

bred. which Ieachtng. conditioned. or research. held fa use in experiments, o r teaching. testing, tests were experiments. conducted TBBBBTh. or involving no surgery but not pain, distress. 01 yet used for such use of pain- Wpo* relieving drugs.

I' (Attech additional sheets cf ns

D. Number of animals upon vhkh experiments. te-~ng. msearch. surgery. or tests were conducted involving ao~mpenying pain w diStreM to tha animals

and for which appropriate anesthetic, analgesic. or tranquilizing drugs were used

-rv or use APHIS FORM 7023AI

E. Number of anirnals upon which teachlng experiments. research. swgery. or testa were conducted involving accompanying pain or distress to the animals and for which the use of appropriate eneathetic, anatgesic. or tranqulliring d ~ g s would have adversely affected the procedures, results, or int- of the leeching. reseam. experiments, surgery. or tests. e n explanatirm of the pmcedures producing pain or distress in those animds and me iwasons such dmgs were not used must be attached to this rwporl)

TOTAL NO

OF ANIMALS

(Cola. C + D + E)

4. Dogs

5. Cats

6. Guinea Pigs

7. Hamsters

8. Rabbits


10. Sheep

11.. Pigs

12 Other Farm An~mals

13 Other Animals

/ I I I


1) Professionallyacceptable standards governing the care, treatment. and use of animals indudlng appropriate use of anesthetic. malgeslc, and tranquilizing drugs, prior to. dur(ng and fdlowlng actual research. teaching, testing, surgery. or experimentatkm were fdlowed by this research facility

2) Each principal Investigator has wnsidered alternatives to palnful procedures.

3) This facility 1s adhering to the standards and regulations under the Ad. and it has required that exceptions to the standards and regulations be speafied and explained by the principal investigator and approved by the Institutional Animal Care and Use Cornmines (IACUC). A summaryof all such exceptions is attached l o this annual rrport.In addition to ldennfying the IACUC-approved exceptions, tMs summary indudes a brief explanation of the exceplinns, aa well as the species and number of animals affected.

4) The attending veterinarindor this research fadlity has appropriate authority to ensure the provision of adequate veterinmy care and lo oversee the adequacy of other aspects of animal care and use.


(Chief Executive Officer or Legally Responsible Institutional Official) I cerWy that the abow is tnm, correct, and complrtr (7 U.S.C. section 2143).

( DATE SIGNED


This report is required by law (7 USC 2143) Fa~lure to report according to the regulations can See attached form for result in an order tc cease and desist and to be subject to penalties as provided for in Section 21! 0 CT 2 I) m1)h addlt~onal information.

Lubee Foundation Inc 1309 N.W. 192nd Ave Gainesville, FL 32609

h

IJNlTED STATES DEPARTMENT OF AGRICULTURE ANIMAL AND PLANT HEALTH INSPECTION SERVICE

I Telephone: (352) -485-1 250

I I

I. REPORTING FACILITY ( List all locations where animals were housed or used in actual research. testing, or experimentation. or held for these purposes. Attach additional sheets if necessary )

1. CERTIFICATE NUMBER: 58-~-0 13 1 CUSTOMER NUMBER: 3082

- -

FACILITY LOCATIONS ( Sites ) - See Atached Listtng

FORM APPROVE OMB NO. 0579-0036

REPORT OF ANIMALS USED BY OR UNDER CONTROL OF RESEARCH FACILITY I Attach additional sheets if necessarv or use APHIS Form 7023A 1 I I I I


Welfare Regulations

B. Number of animal bemg bred. conditioned, or held for use in teaching. testing. experiments, research, or surgery but not ye used for such

5. Cats

6. Guinea Pigs

7. Hamsters .

N / A

8. Rabbits - -. -

f N / A

9. Non-human Primates N / A 10. Sheep N / A 1 1. Plgs

-- N / A 12. Other Farm Animals N / A

C. Number of animals upon which teaching. research, experiments, or tests were conducted involving no pain. distress, or use o pam-relieving drugs

13. Other Animals

D. Number of animals upon which experiments, teaching, research, surgery. or tests were conducted involving accompanying pain or distress to the animals an for which appropriate anesthetic, analgesic, or tranquilizing drugs were used.

I

E. Number of animals upon which teaching, experiments. research, surgery or tests were conducted involving accompanying pain or distress to the animals and for wh the use of appropriate anesthetic, analgesic, or tranquiliz drugs would have adversely affected the procedures, res or interpretation of the teaching, research, experiments, surgery, or tests ( An explanation of the procedures producing pain or distress in these animals and the reasc such drugs were not used must be attached to th~s report



99 I None .-j--.-zg9

ASSURANCE STATEMENTS I

1) Professionally acceptable standards govemmg the care, treatment. and use of animals. including appropriate use of anestetic, analgesic, and tranquilizing drugs. prior to, during. and following actual resc teachlng testlng, surgery or experimentation were followed by this research facility.

0

I 2) Each principal investigator has considered alternatives to painful procedures

None

3) This facility is adhering to the standards and regulations under the Act, and it has required that exceptions to the standards and regulations be specified and explained by the principal investigator and ap lnstitut~onal Anmal Care and Use Committee (IACUC). A summary of all such exceptions is attached to this annual report. In add~tion to identifying the IACUC-approved exceptions, this summary in( brlef explanation of the exceptions, as well as the species and number of animals affected.

4) The attending veter~narian for this research facdity has appropr~ate authonty to ensure the provision of adequate veterinary care and to oversee the adequacy of other aspects of animal care and use


USDA Annual Report 2003-2004

Date: 1 October 2004

1) Animal Inventory as of Sept. 30,2004: 267 bats and 4 Sugar gliders

2) Animal deaths 1 Oct 03 - 30 Sept 04: 20 bats (~nimals held, but not used for research)

3) Facility Location: Lubee Foundation, Inc. 1309 NW 192" Avenue Gainesville, FL. 32609 r j . ~ & ~ ~ ~ . ~ b b ~ .

The following buildings at the facility: a) Core Building b) Bat Observation Building c) Small Bats Building d) Quarantine Building

Interagency Report Contr w See attached form for additional information.

e

Th,s repoi is required by law (7 USC 2143). Failure to report according to the regulations can



I L C ,

.-


Everglades Habitat Preservation Inc

result in an order to cease and desist and to be subject to penalties as provided for in Section 21!



P 0 Box 2326 La Belle, FL 33975

-

I, or experimentation, or held for these purposes. Attach additional sheets if necessary ) 1 3. REPORTING FACILITY ( List all locations where animals were housed or used in actual research, testing r


REPORT OF ANIMALS USED BY OR UNDER CONTROL OF RESEARCH FACILITY I Attach additional sheets If necessarv or use APHIS Form 7023A 1 I

- -- - --


-

C. Number of an~mals upon whch teaching. research, experiments, or tests were conducted involving no p a n distress, or use o pain-relieving drugs.

- - --






5. Cats

6. Guinea Pigs

7. Hamsters

8. Rabbits I 9. Non-human Primates I 10. Sheep I 11. Pigs I 12. Other Farm Animals

& 4 . 1 ~

ASSURANCE STATEMENTS I 1 ) Professionally acceptable standards governing the care, treatment, and use of animals, including appropriate use of anestetic, analgesic, and tranquilizing drugs, prior to, during, and following actual resc



3) This facility is adhering to the standards and regulations under the Act, and it has required that exceptions to the standards and regulations be specified and explained by the principal investigator and ap lnstitut~onal Animal Care and Use Committee (IACUC). A summary of all such exceptions Is attached to this annual report. In addition to identifying the IACUC-approved exceptions, this summary in( brief explanation of the exceptions. as well as the specles and number of animals affected.



) DATE,SIGN~D 1

1. REGISTRATION NO. FORM APPROVED

OMB NO. 05794038

2. HEADOUARTERS RESURCH FACLIW (Name and Address, as registered wdh USDA Include Zip Code)

., This report is required by law (7 USC 2143). Failure to report according l o the regulalions can so0 r w ~ u rid. lor Intaragency Report Control No. *e+i in an order lo kease and desist and to be subjecl l o penalties as provided lor in &lion 2150. addiliond inlormetion. 01 80-00A-AN

*.

CONTINUATION SHEET FOR ANNUAL REPORT O f RESEARCH FACILITY

( TYPE OR PRINT)

Animals Covered By The Animal condil~oned, or

Welfare Regulations accompanying pain or

------------------ 12 &/OR 13. Other

I

I 1). Prolessionally acceplihle slondards governing the cate. treatmenl. and use 01 animak. including approriate use of afteslhet~c, analgesic, ant1 tro*~quilizing drltgs, prior to, c l l ~ r ~ l q ,

and lollowiry actual research, teach~ng, tesllng. surgery, or elcpertmenlallon were lollowed by this research lactltly.

2). Each prirwpal ~nvesligator has considered alternalives to painlul procedures

3). This facility 1s adhering to the standards and regulal~ons UMer the Act, and 11 has required Ihat exceplions l o Ihe standards and regulal~ons be specified and explained by the principal invesl~gator and approved by the Ins l~ lu t i o r~~ I Animal Care afd Use Colmwttee (IACUC) A summary of all such exceptions is at lachcd to this annual report In addition l o ~denlily~ng Ihe IACUC-approved exceptions, th~s sunlmary lncludes a br~el explanallon of the excepllons, as well as Ihe speoas ant1 nurrlber ol animals allected

4). The attending velerinar~an for lhls research lacilily has ap(Popriate aulhor~ly to ensure the provlsio~~ of adequale veleriltary care and to oversee the adequdcy of other aspects 01 antmal care and use.

CEHTIFICATXON BY IIEADQUAKTES RESEARCH FACILITY OFFICIAL (Chief Executive Officer or Legally Responsible Institutional Oficial)

I re r t~ l y that the above IS true. correcl. and cornplele (7 U S.C Sect~on 2143)

SGNANRE OF C.E.O. OR INSTITUTIONAL OFFICIAL I NAME T ~ E OF C.EO- OR INSTITUTIONAL OFFK~IAL nype or P ~ I I U I DATE SIGNED

This report is required by law (7 USC 2143). Failure to report according to the regulations can 2 3 2DO4 see attached form for result in an order to cease and desist and to be subject to penalties as provided for in Section 21! additional information.


I Imperial Point Animal Hospital 5141 Ne 17th Ave Fort Lauderdale, FL 33316

I. CERTIFICATE NUMBER: 5&&0 133




I 3. REPORTING FACILITY ( List all locations where animals were housed or used in actual research. testing, or experimentation, or held for these purposes. Attach additional sheets if necessary )

FACILITY LOCATIONS ( S~tes ) - See Atached Listing

REPORT OF ANIMALS USED BY OR UNDER CONTROL OF RESEARCH FACILITY I Attach additional sheets if necessarv or use APHIS Form 7023A 1 I rn




F.



B. Number of animal being bred, conditioned, or held for use in teaching, testing. experiments,


Welfare Regulations research, or surgery but not yc used for such purposes. I

4. Dogs

5. Cats

6. Guinea Pigs I I 8. Rabbits


10. Sheep

11. Pigs


13. Other Animals I I I


1 ) Professionally acceptable standards governing the care, treatment, and use of animals, including appropriate use of anestetic, analgesic. and tranquilizing drugs, prior to, during, and following actual resc teaching, testing, surgery, or experimentation were followed by this research facility.


3) This facility is adhering to the standards and regulations under the Act, and it has required that exceptions to the standards and regulations be specified and explained by the principal investigator and ap Institutional Animal Care and Use Committee (IACUC). A summary of all such exceptiom is attached to this annual report. In addition to identifying the IACUC-approved exceptions, this summary inc brief explanation of the exceptions, as well as the species and number of animals affected.


- -

1 DATE SIGNED 1

APHIS FORM 7023 (Replaces VS FORM 18-23 (OCT 88), which is obsolete.)

( AUG 91 )

UNITED STATES DEPARTMENT OF AGRICULTURE ANIMAL AND PLANT HEALTH INSPECTION SERVICE I 1. CERTIFICATE NUMBER: 58-R-0 1 34

CUSTOMER NUMBER: 26748 I FORM APPROVED OMB NO. 0579-0036

ANNUAL REPORT OF RESEARCH FACILITY ( TYPE OR PRINT ) I

P 0 Box 2326 La Belle, FL 33975

--------------- ------- -------------- I ---- ------------------ I I

. REPORTING FACILITY ( List all locations where animals were housed or used in actual research, testing, or experimentation. or held for these purposes. Attach additional sheets if necessary ) 1 FACILITY LOCATIONS ( Sites ) - See Atached Listing

4. Dogs -9 5. Cats I I

6. Guinea Pigs I I -

7. Hamsters I I 8. Rabbits I I 9. Non-human Primates I 10. Sheep I I 11. Pigs


C. Number of animals upon which teaching, research. experiments, or tests were conducted involving no pain, distress, or use a pain-relieving drugs.



REPORT OF ANIMALS USED BY OR UNDER CONTROL OF RESEARCH FACILITY I Attach additional sheets if necessaw or use APHIS Form 7023A 1 I


A.


Welfare Regulations ( COLUMNS

i C + D + E )


1) Professionally acceptable standards governing the care, treatment, and use of animals, including appropriate use of anestetic, analgesic, and tranquilizing drugs. prior to, during, and following actual resc

8. Number of an~mal bemg bred. condit~oned, or held for use ~n teachlng, testmg, expenments, research. or surgery but not ye used for such purposes.


I


3) This facility is adhering to the standards and regulations under the Act. and it has required that exceptions to the standards and regulations be specified and explained by the principal investigator and ap Institutional Anlrnal Care and Use Committee (IACUC). A summary of all such exceptions is attached to this annual report. In addition to identifying the IACUC-approved exceptions, this summary in( brief explanation of the exceptions, as well as the species and number of animals affected.



APHIS FORM 7023 (Replaces VS FORM 18-23 (OCT 88), which is obsolete.)

SIG

( AUG 91 )

DATE SIGNED

(b)(6), (b)(7)c

I. Number of an~mals bemg bred. cond~t~oned, or held tor use In teachmg, testing, experlments, research. or surgery but not yet used tor such purposes.

8 ,:> 2 - Department of veterans A£ f airs Headquarters 810 Vermont Avenue, NW Washington, DC ,,, q 4 2 0 .\ . $ 2 . . , I

C Number of animals upon wh~ch teach~ng, research, experiments, or tests were conducted involvmg no paw, distress, or use ot paln- relieving drugs.

D. Number o l an~~na ls upon which experiments, . teaching, research, surgery, or tests were conducted ~nvolving accompanymg pam or distress to the animals and for wh~ch appropriate anesthetic, analgesic, or tranquilizing drugs were used.

E. Number 01 an~mals upon whlch teach~ng, experlments, research, surgery or tests were

'

conducted ~nvolvmg accompanying pam or dlstress to the anmals and lor wh~ch Ihe use of appropriate anesthetic, analges~c, or t ranqu~l~z~ng drugs would have adversely affected the procedures, results, or ~nterpretat~on of the teachmg, research,

.experiments, surgery, or tests (An explanatron of the procedures producrng pain or dtstress m these anrmals and the reasons such drugs were not used must be attached to this report)


L

Th~s report IS requ~red by law (7 USC 2143) Fa~lure to report accord~ng to the regulat~ons can DEC 072004

See reverse s~de tor Interagency Report Control NO

result tn an order tocease and des~st and l o be subjecl l o penalt~es as prov~ded for III Sectlon 2150 , , , , add~t~onal mtormat~on 01 80-DOA-AN :Lr

3 3 1 ' > < I '

(Cols. C + D + E)

. L ,. " FORM APPROVED

,. , OM6 NO 0579-0036

3 UNITED STATES DEPARTMENT OF AGRICULTURE ' *

ANIMAL AND PLANT HEALTH INSPECTION SERVICE - ,

- , 1. REGISTRATION NO.

58-v-003 : 43/. r '+;- .

la/c . . C I

- - . - - ,- -.- , -...%-

- ' ,. -.. -- - * % r 4, , c , -;+ ,-t 416.;: 3


. 3 T . ,

2. HEADQUARTERS RESEARCH FAC!LIP (Name and Address, as registered wrth USDA, . I jncltde&7~(2@$l Z t r : s ; ! : - 4 ~ ~

I

3. REPORTING FACILITY (L~st all locat~ons where an~mals were housed or used In actual research, testmg, teach~ng, or experlmerttatlor~, or held tor these purposes- Attach addltlonal sheets 11 necessary ) -

FAClLlTV LOCATIONS (Sites)

VA Medical Center (516)

Bay Pines, FL 33744 REPORT OF ANIMALS USED BY OR UNDER CONTROL OF RESEARCH FAClLlTV (Altach ~cirdrtror~al sheets if necessarv or use APHIS FORM 70234)

m

-

A

Anmals Covered By The Anlmal

Welfare Regulat~ons

0

4. Dogs

5. Cats

6. Gumea P~gs

7. Hamsters

8. R a b b ~ t s

9. Non-human Pr~rnates

10. Sheep

11. Plas

12. Other Farm An~mals

13. O t h e r An~mals

RATS


- -

- - m

1) Protess~oc~ally acceptable standards governmg the care, treatment, and use 01 anlmals. 111clud1ng approrlate use o l anesthet~c, analges~c, and tranquhr~ng drugs, pr~or to, dur~ng, and lollow~ng actual research, teach~ng, testmg, suryery. or exper~mer~tat~on were lotlowed by lhls resedrch taclllty

2) Each prmc~pal lnvest~yator has c:ons~dered alternal~ves to pa1111ul procedures

3) Th~s t ~ c ~ l ~ t y IS adhermy to the standards and regulations under the Act, and 11 has requ~red that exceptlons lo the staodards and regulat~ons be specitled and expld~ned by the prlnc~pal Investtgator and approved by Ihe Inst~tut~onal A n ~ ~ r ~ a l Care and Use Co~n~n~ t t ee (IACUC) A summary of all such exceptions i s at tached t o thrs annual report. In a d d ~ l ~ o n to ~den l~ tymg the IACUC-approved exceptions, this sulrlrnary ~ncludes brtel expldoat~on ol !he exceptlons, as well as !he specles and number 01 a n ~ ~ ~ l a l s aflected

4) The a t te r~d~ng veterlnarlacl for t h ~ s research lac~l f ty has approprldte duthor~ly l o ensure the provlslo~l of adequate veterlndry care and to oversee the adequacy o l other aspects of dllllndl Lare and use

CEKTIFICA'I'ION BY 1IEAI)QUAHTKS RESEARCH FACII.ITY OFFICIAI, (Chief Executive Officer or Legally Itesponsible Institutional Official)

I c.ert~fy lhat the above IS true. corre~t. a d co~nplete (7 U S C St?c.t~on 2143)

I

I

) e or Prrr~t) DATE SIGNED

//- 3-4/

. ' - ? " . :

APHIS FORM 7 g 3 (Replaces VS FORM 18-23 (OCT 881, whtch 1s obsolete )

(AUG 91)

PART 1 - HEADQUARTERS

' I I ' 3. REPORTING FACILIT* (L~st all l w a t m r where an~malr were housed or used in actual research. Iestlng, teshtng, a exper~meltMton, or held lor these p u r p o ~ ~ i . Attach addltlonal

sheets 11 necessary )

FAClLrrY LOCATIONS (Sites)

I

This report 1s requtred by law (7 USC 2143) Falure to report accord See reverse stde for Interagency Report Control No

result ~n an order to cease and des~sl and l o be subject to penalt~es as p ' o addtttpnal inlormalton 01 80-WA-AN 1 , L ' 1 ,

VA Medical Center1546

a . UNITED STATES DEPARTMENT OF AGRICULTURE - ' 1. REGISTRATION NO. ' . , .',. '+I ' 1) .: : ANIMAL AND PLANT HEALTH INSPECTION SERVICE 4; ' a 1 i

58-v-005 %, , .

" ',, ' ~ O R M APPROVED a '

, OMB NO 0579-0036,

,. . '=". * ' - ' . . - 2. HEADOUARTERS R E S F H 3 F A S I L Y (Name and fddress. as registered with USDA include Zip Cqde) ;* r ' '. '.+d -. - "

C *.

Department o f ~ete$i&s A f f a i r s ANNUAL REPORT OF RESEARCH FACILITY

(TYPE OR PRINT) 810 Vermont Ave. NW Washington, DC 20420

I

13. O t h e r Animals

ts

Mice

F.

TOTAL NO OF ANIMALS

(Cols. C + I) + E)

0

0

5

0

3

0

0

0

0 I

2) Each p r~nc~pa l mvesttgalor has cons~dered allernat~ves to p a ~ ~ ~ f u l procedures

3) Tha l a c ~ l ~ t y IS adhermy to the standards a i d r e g ~ l ~ t t o ~ ~ s under ihe Act, and II has requ~red that excepl~ons to ihe sta~~dards and regulal~ons be spec~ l~ed and explrned by the p r ~ n ~ ~ p a l mvestlgator and approved by the lna t~ la tu~ la l Arwsal Care and Use Co~nm~ltee (IACUC) A summar). of all such exceptions i s a t tached t o t h ~ s annual repor(. In addlt~on to ideol~fytng the IACUC approved exceptlons, t h ~ s sulnmary ~ncludes a b r~e l expldnal~on of the exceplans. as well as the spectes and number 01 an~mals attested

4) Tt,e a l l and~~ lg velerwandl lor l h ~ s resedrch t ac~ l~ i y has approprlale aulhor~ly to ensure the provlsloll ot adequate veler~nary Care and l o oversee the adquacy of olher aspects o l dnltnal Lare a11d use

CEHTIYICA'l'ION BY 1IEAI)QUAU'TES RESEAHCll FACII.11'Y OPFICIAI. (Chief Executive Officer or Legally Responsible Institutional Ofticial)

I c.ert11y Ihal Ihe abt,ve IS true. correct. and co~nplele (7 U S C Sec.l~on 2143)

PART 1 - HEADQUARTERS

necessan or use APHIS FORM 7023*)

E. Number of an~mals upon wh~ch teach~ng. experiments, research, surgery or tests were conducted ~nvolvtng accompanytng p a n or dtstress to the antmals and tor wh~ch the use of appropriate anesthettc, analgestc, or tranqutllztng drugs would have adversely affected the procedures, results, or tnterpretatan of the teach~ng, research. exprlments, surgery, or tests (An explanation of the procedures prodUcfl1y pain Or these animals and the reasons such drugs were not used must be attached to this report)

0

0

0

0

0

0

0

0

0 I

REPORT OF ANIMALS USED BY OR UNDER CONTROL OF


I ) Pruless~oaally acceptable standards governing the care, treatment, and use of animals. including approriale use ot anesthetic. analgesic. and tranquilizing drugs. prior lo. duriilg. m d tollowng actual research, leach~ng, testing, surgery, or exper~menlalion were lollowed by thts research lacility.

100

557

(1

SIGNATURE OF C.E.O. OR INSTITUTIONAL OFFICIAL NAME B TITLE OF C.E.O. OR INSTITUTIONAL OFFICIAL (7ype or Prirlt)

.' t

RESEARCH FAClLlT*

DATE SIGNED

k/ &. . .

496

90

(1

A

(Attach ddid~tinnal sheets I

306

303

A

An~mals Covered By The An~mal

Welfare Regulattons

'

4. Dogs

5 . Cats

6. Gumea PIQS

7. Hamsters

8. Rabb~ts


10. Sheep

11. P~as

I

B. Number of an~mals betng bred, cond~t~oned, or held lor use ~n teachtng, testing, wper~ments, research, or surgery but not yet used tor such purposes.

0

0

0

0

0

0

0

n

C Number 01 an~mals upon which teachtng. research, experiments, or tests were conducted ~nvolv~ng no Pam, dfilress, or use of pan- reltevmg drugs

0

0

5

0

3

0

0

n 0

160

28

0

allllnall ulun wh~ch experc~rlenls, teachtng, research. surgery, or lesls were conducled t~wolvtng accompalrytng patn or dlslless the alllmals

which approprlale and lor anesthetu, al,algeslc, or tral,qulllzlng used

0

0

0

0

0

0

0

o 0

1,962

420

108 - -

' . - Thrs report ts requ~red by law (7 USC 2143) Failure to report according to the regulations can See reverse side lor Interagency Report Contrd No

r d t in an ordec to cease and desist and to be subject to penaltres as provrded lor in Section 2150. additional informatcon. 01 80---AN


FORM APPROVED OM6 NO. 05790036

DEPARTMENT OF VETERANS AFFAIRS 810 VERMONT AVENUE, NW WASHINGTON, DC 20420

8

3. REPO#IING FACILITY (Ltst aU bcat~ons where annnak were housed or used tn actual research, tesltng. teaching, or awper-tation, or heid tor these prposer. Al l lch add~tmnal sheers it necessary.)

1601 SW ARCHER ROAD I

Animak Cavered 6 y The Animal

Welfare Regulations

antmak bemg bred, ~ t ~ . or held tor use In teaching. testing, experiments. research. M surgerybutlmI yet used for such purposes.

GAI-- PT. - 32608 1 REPORT Of: ANIMALS USED BY OR UNDER CONTROL OF RESEARCH FACtLCCY (Mach adidifrorral sheets if necessary or use APHIS FORM 702311) -

A l B. umber of c Number ot D. N~~~~ aolmals E Number of mmaJs upon which teaching. experiments, research, surgery or tests were

F. antmak upon whlch experctnents. whach leachmg* teachrng, research. conducted invdnng accompanying pain or distress research. surgery, or tests were to the anrmds and for wbiih the used appr-te TOTAL NO exPerl-ts- or conducted tnvdving anesthettc, analgesic, or tranquiirzing drugs would OF A N ~ M A ~ tests were accompanytng pan or have adversely atfected the procedures. results. or conducted di.;trers to the arlrnwfs lnterpretatlon of the teaching. research. inrdvinpn~ and which appropriate exPerImentS, SUrWY= 01 tests. (h a ~ l a n - d (-I. C + pan, distress, or a,tbtlc, analg-, Procedurm producing W n ff them? 0 + E) useofpaul- tranquilizing drugs were animals end the reasons such drugs were not used relbevtng drugs. used mud be atiached to this repod).

--

4. Dogs 0

5: Cats 0

6, eu-ilea Pigs 0

7. wste rs 0

8. ' Rabbrt~ 4


10. Sheep 0

11. Pias 0

13. O W Animals

1 ASSURANCE STATEMENTS --

1) P r o l e s s ~ ~ ~ I l y acceptable standards governtng the care, treatment, and use of anrmals, tnctuding ~pproriate use o l anesthetic. analgesic. and tranqutlrztng drugs, pww to. dunng. and fdlowtg actual research, teachtg. lestmg, urryery. or experrmentatlon were followed by thts resedrch tactlity

2). Each prtncipal investrgator has conudered dternal8ves to pattrful procedures

3). This faciltty is adhering to Ihe standards and regu&tums under the Act, and it has required that exceptions to the standards and regublions be specified and explained by the ptncrpal investigator and approved by the Institutional Antmat Care and Use Committee (IACUC). A sumnlary of all SUCh exceptl045~ k attached to this-annual report. In addilion to identifying the IACUC-approved exceptions. this summary includes a brief explanation of the exceptions, as well as the species and number of an~rnals atlected.

4) The attend~tq vetertnarwtr lor this rese~rch tacrltty has appropiate authortly to ensure the provlslo~~ ol Jdeqlwte veterinary care and to orersee the adequacy of olher aspects of atltcnal care and use.

CEHTIFJCA'I'ION HY IIEADQUAK'I'ES HESEARCIS PACI1,ITY OFFICIAI, (Chief Executive Offacer or Legally Responsible Institutional Ofh5al)

I certrfy that the above is true, correct. dtd cotnplete (7 U S C Sectmn 2143)

SIGNATURE OF CEO. OR INSTITUTIONAL OFFICLAL NAME 8 TITLE OF C.E-0. OR INSTITUTIONAL OFFICIAL fly* or P~IIU)

)I

1

(AUG

i ha - usda-aphis · aphis form 7023 (replaces vs form 18-23 (oct 88). which is obsolete part 1 -...

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