hyola 404rr consolidated version july 4th 2013

8/12/2019 Hyola 404RR Consolidated Version July 4th 2013

1/31

Hypothesis

Accepted/Reje

cted

Data collected that

supports the

conclucion (please

add info in separate

document)

1. Germination was the biological issue for the badstand of plants and the origin was:

a.Bad germination in the seed at processing plant reject refer tab 1a

rejected

CONCLUCION REJECTED

b.Bad germination due bad transport/storagemanagement. reject refer tab 1b

rejected

CONCLUCION REJECTEDc.Bad germination due to toxicity effects of Syngenta(XXXXX?) seed treatment.

reject refer tab 1c

?

CONCLUCION ?????

d.Bad germination due to toxicity effects of Bayer

(Jockey) seed treatment.reject refer tab 1d

?(likely) (part of issue only)


2/31

CONCLUCION

?????

2. Emergency of good quality seed was the biologicalissue for the bad stand of plants and the origin was:

a.Reduced vigor or hypocotyl length due to toxicityeffects of Syngenta (XXXXX?) seed treatment.

reject refer tab 2a

?

CONCLUSION ??????

b.Reduced vigor or hypocotyl length due to toxicity

effects of Bayer (Jockey) seed treatment.

refer tab 2b - further

information required

? (likely)

CONCLUCION?????

c.Low vigor or hypocotyl length due to varietal (hybrid)effect.

reject refer tab 2c


3/31

rejected

performance of the

hybrid in prior yearsin field trials

CONCLUCION ???????

d.Low vigor or hypocotyl length due to environmentaleffects from the production area (Canada). Seed lot

effect

further information

required

?

CONCLUCION

e. Interaction a x c or b x c. Or a x d or b x d

likely

3. Emergency of bad quality seed was the biologicalissue for the bad stand of plants of plants and the origin

was:

covered in 2

above

a.Reduced vigor or hypocotyl length due to toxicityeffects of Syngenta (XXXXX?) seed treatment.

refer tab 3a

CONCLUCION

b.Reduced vigor or hypocotyl length due to toxicity

effects of Bayer (Jockey) seed treatment.refer tab 3b

CONCLUSION ??????


4/31

c.Low vigor or hypocotyl length due to varietal (hybrid)effect.

refer tab 3c - further

information required

CONCLUCION

d.Low vigor or hypocotyl length due to environmentaleffects from the production area (Canada).

refer tab 3d

CONCLUSION ??????e.Interaction a x c or b x c.

f.Presence of fungus in the seed that is not controlled

by Jockey.reject

Seed from OS

production (untreated

& jockey treated), Aus

production (untreated

& Jockey treated) &

production from Aus

prod last season were

tested by

Government

pathologist and found

normal levels of fungi

and bacteria with no

differences in types &

levels of pathegons

between five

samples.

CONCLUSION REJECTED

4. A combination of any of the above was the reasonfor the bad stand of plants.


5/31

Data needed to be collected

Responsible Location

Sue Toowoomba

Jim

Sue Toowoomba

Jim

Sue Toowoomba

Jim

We need to discuss

questions in the right

Sue Toowoomba

Jim


6/31

Need need to discuss

questions in the right

Sue Toowoomba

Jim

Need to get more clarity on

the info or extra info

Test the effect of chemical (Jockey)

on hypocotyl length and seedling

emergence on thermogradient plate

at 4 temperatures (range 10-25deg

C) using treated and untreated seed.

Assessment to include germination

count & measurement of hypocotyl

length @ 4,7 & 10 days

Sue Toowoomba

We need to treat seed with these

chemicals alone and in combination.

Based on experience of other

species we should look attemperature /chemical interaction

or we risk missing the impact. Rate

of seed deterioration affects should

also be investigated. Ideally a dose

series would be helpful but not

essential to keep the experimental

size manageable in the first cycle. Jim

Need to get more clarity on

the info or extra info

Sue Toowoomba


7/31

We need to review in-house testing

done prior to implementation of

routine seed treatment. (GGS

records)I expect GGS would have

looked at both temperature and

dose affects. We may have moved

onto new genotypes post theoriginal work? JIm

We need to run test that

answers to the question in

column G. We also need to

run this trial with and

without bath chemicaks

independently.

Test hypocotyl length and seedling



C) of Hyola 404RR Xaustralian prod

and Canadian Prod. Assessment to

include germination count &

measurement of hypocotyl length @

4,7 & 10 days

Sue Toowoomba

Jim

AGREE ON NEEDED TRIALS

Sue Toowoomba

Jim

Jim

Sue Toowoomba

Sue Toowoomba


8/31

Test hypocotyl length and seedling



C) of Hyola 404RR and Hyola 555TT.

Assessment to include germination

count & measurement of hypocotyl

length @ 4,7 & 10 daysSue Toowoomba

Sue Toowoomba

Sue Toowoomba

Sue Toowoomba


9/31

Comments

the germinations are acceptable on arrival from Canada in the pacseeds lab and these are consistent with OIC's done

before shipment from Canada. Germination post treatment in Toowoomba do not show any, or no significant seed

deterioration.

Only one data from Canada was presented. Although, see that germination at Australia

was OK which supports that fact that was OK in Canada also. What means the variable

"quantity per treatment (mt)"?

The germination on samples returned from WA are lower than you could reasonably expect even if the conditions

during shipment were extreme. Note that moisture on returned samples are consistent with moisture of

preshipment samples so we can infer seed moisture did not increases due to conditions during shipment. Hence any

accelerated deterioration during transport would have to be through a temperature impact. Using the published

seed deterioration equations even extreme (but possible) temperatures during shipment are insufficient to explain

the observed fall in germination. That means another factor is involved- the most likely other factor is phytotoxicity.

Given the returned samples are showing reduced germination it is more than likely the source of phytotoxicity is one

of the applied chemicals. However the reduced germination itself appears insufficient to produce the poor field

emergence, the phytotoxic effect is probably affecting more than germination and related vigour.

Very strong supportative data

the germination and vigour hypothesis can be explored in the same experiments and as germination and vigour are

related this aspect is easy to combine. Hypocotyl length can be captured in the same tests with appropriate testing

methodology

Last test was done in May 13th. When seed was planted? Before? ...if afterhow many

days? What means batches MX and JX?

The seed deterioration that occurred during shipment/ storage in WA is more than can be explained by standard

temperature and moisture driven deterioration. In addition untreated samples stored in Toowoomba deteriorated at

a rate predicted by the seed deterioration equations, whilst it did not predict seed deterioration in treated seed

stored either in Toowoomba (seed store) or shipped to WA. The chemicals appear to be the only variable to explain

this difference, but there is no direct evidence(?) to separate the two chemicals


10/31

Last test was done in May 13th. When seed was planted? Before? ...if afterhow many

days? Only two batches were treated with Jockey? All 404RR seed was terated with

Jockey + Syngenta agrochems? No 404RR untreated seed was sown?

Table 2a does not shows seed Quality info nor Vigor or hypocothyl length. How do we

know tat the seed was f good quality"?

Table 2b does not shows seed Quality info nor Vigor or hypocothyl length. How do we

know tat the seed was f good quality"?


11/31

No data is presented showing thet 404RR has same hypocotul length as any other

ccommercial material

It is at least possible if not probable that the phytoxic effect is more pronounced on some seeds in the lot than

others. This may be because of seed coat damage, thin seed coats (there is significant size variation within the lot,larger seeds are likely to have thinner seed coats allowing the chemical to slowly enter the seed. The seed lot also

has a reasonable % of green seed. Green seed is know to be more permeable, so may allow chemical entry over time.

EXPLAIN 3B DATA. WHY SO LOW GERM DATA?


12/31

EXPLAIN 3C DATA. WHY SO LOW GERM DATA?

EXPLAIN 3B DATA. WHY SO LOW GERM DATA?


13/31

proposed testing methodologies

background:

The germination data indicates the seed was good quality before treating and immediately thereafter

Seed lot contains a high % of green and damaged seed, and seed of variable size

Jockey is know to affect hypocotyl length and to affect damaged seed more than sound seed

Green seed is known to be more permeable

Large seed is likely to have thinner seed coats and may therefore be more permeable

Germination and vigour tests post delivery to WA show significant deterioration but not sufficient to exp

Germination and vigour tests do not take hypocotyl length into account in seedling classification but vari

Germination temperature is known to profoundly affect expression of phyto toxic affects and hypocotyl l

The syngenta chemicals cruiser and maxim have not had adverse comments in literature re emergence.

The more likely cause of the observed problems is:

Hypothesis 1:

Jockey adversely affects hypocotyl length and this results in reduced and late emergence in fields where

This effect is not observed in lab tests because they do not routinely take account of hypocotyl length. It

Hypothesis 1A

Jockey adversely affects hypocotyl length of some seeds in the seed lot and this results in reduced or lat

Hypothesis 2 and 2A

As above but Synngenta chemical is responsible

Hypothesis 3 and 3A

interaction of jockey and Syngenta cause the effect.

Testing methodology

A key field observed feature is hypocotyl length, any testing should closely examine this trait

To better manage work loads and "iron out" testing methods I suggest testing the hypotheses one at a ti

Given jockey is the prime suspect I would do it first.

chemical seed lot germination conditions

roll towels single marked row 100(?) seeds per toweltemperatures treatments to cover range above and belo

10 15 20 25

nil Canada lot as delivered

" green seed

" non green

" damaged

" sound black

other seed lot


14/31

other variety**

jockey Canada lot as delivered

" green seed

" non green

" damaged

" sound black

other seed lotother variety

(jockey treatment at recommended rate lab treated)

Collected data- hypocotyl length. (approx 5mm increments), germ abnormal etc. Pay particular note of o

test duration

subject to final temps- approx same total heat units for each temp. Hence 20deg 10 days, 10 deg 20 day

4 roll towels per treatment (total 400 seeds). Completely randomized design.

reference set of green seeds, damaged kept for future reference

* if soil temp data is unavailable use ambient(avg max,min) for 2 weeks prior and 2 weeks post plantings

** If seed available from seed lot planted along side canadian seed in WA consider using this.


15/31

lain all of the field observations

ation for this was noted in some tests of treated seed lots. Not clear if there were 2 classes (very short a

length in other species.

pposite in case of metalaxyl and thiamethoxam, neutral for other components.

planting depth is deeper, or there is some soil crusting, other field factors (temperature) may increase th

is likely the effect becomes more pronounced during post treatment storage so is not observed in tests i

e emergence in fields where planting depth is deeper or there is some soil crusting. Other field factors (t

me.

likely field temperatures (if field likely is 20 then range for tests would be approx 10- 30)*

30 (actual temperatures as fit the plate- range is goal not actual temperatures)


16/31

ther specific abnormalities such as stunted roots.

, 30 deg7 days

(or month prior and month after)


17/31

nd normal) or a continuum.

e effect

mmediately after treatment.

mperature) may increase the effect.


18/31

Quantity per

treatment (mt)Grower Batches OIC (Pre shipment)

Original Germ Jan

13

Germ May 13 (retained

QA sample)

52 Hytech Untreated 96 96.5 94.1

60 Blend Untreated 97.5 97

18.44 Hardy L1 Untreated 94 95

20.5 Millner Untreated 97 93

18.58 Hickey L3 Untreated 98 97

57.14 Murna Untreated 95 95

Hyola 404RR


19/31

Quantity per

treatment (mt)Grower Batches

Original Germ

Jan 13

Germ May 13 (retained

QA sample)

Germ May 13 (sample

from

transport/storage)

52 Hytech JX 94.5 89 81.75

60 Blend MX 95.2 93.3 89.9

18.44 Hardy L1 MX 97 96

20.5 Millner MX 96.8 93.1 91.5

18.58 Hickey L3 MX 95 95.5

37.14 Murna MX 96.25 94.3 94

20 Murna JX 93.5 94.5

NB Germ results = average of germs

Hyola 404RR


20/31

Quantity per treatment

(mt)Grower Batches

Original Germ

Jan 13

Germ May 13

(retained QA

sample)

Germ May 13 (sample

from

transport/storage)

60 Blend MX 95.2 93.3 89.9

18.44 Hardy L1 MX 97 96

20.5 Millner MX 96.8 93.1 91.5

18.58 Hickey L3 MX 95 95.5

37.14 Murna MX 96.25 94.3 94


Hyola 404RR


21/31

Quantity per

treatment (mt)Grower Batches

Original

Germ Jan 13

Germ May 13

(retained QA

sample)

Germ May 13

(sample from

transport/storage)

52 Hytech JX 94.5 89 81.75

20 Murna JX 93.5 94.5


Hyola 404RR


22/31

Quantity per

treatment (mt)Grower Batches CST Jan 13

"CST" May 13

(retained QA

sample)

"CST" May 13

(sample from

transport/storage)

AA Jan 13"AA" May 13 (retained

QA sample)tr

Hardy L1 Untreated 93 97 85 85

18.44 Hardy L1 MX 92 95 75 80.5

Millner Untreated 93 94 87 90

20.5 Millner MX 90.6 91.6 79.5 82 84.6

Hickey L3 Untreated 95 90 94 90

18.58 Hickey L3 MX 95 90 90 87 87

Murna Untreated 95 93 88 88

37.14 Murna MX 92.3 89 80.6 83.3


Hyola 404RR


23/31

Quantity per


"CST" May 13

(retained QA

sample)

AA Jan 13"AA" May 13

(retained QA sample)

Murna Untreated 95 93 88 88

20 Murna JX 92 88 76 82


Hyola 404RR


24/31

Hyola 404RR

Quantity per


"CST" May 13 (retained

QA sample)

Hardy L1 Untreated 93 97

18.44 Hardy L1 MX 92 95

Millner Untreated 93 94

20.5 Millner MX 90.6 91.6

Hickey L3 Untreated 95 90

18.58 Hickey L3 MX 95 90

Murna Untreated 95 93

37.14 Murna MX 92.3 89

20 Murna JX 92 88

Hyola 555TT

89mt Hytech Untreated 94

Hytech MX 91

Hytech JX 93

Hyola 575CL

Frontier L1 Untreated 93

50 JX 95.6



25/31

"CST" May 13 (sample

from transport/storage)AA Jan 13

"AA" May 13

(retained QA

sample)

"AA" May 13

(sample from

transport/storage

)

85 85

75 80.5

87 90

79.5 82 84.6 77.5

94 90

90 87 87 86

88 88

80.6 83.3

76 82

89.25

67

78

96

94.6


26/31

Quantity per treatment

(mt)Grower Batches CST Jan 13

"CST" May 13 (retained

QA sample)

"CST" May 13 (sample from

transport/storage)AA Jan

Frontier + Hytech Blend Untreated 92.5 89 93.1

46.5 + 5.5 Blend MX 90 92 54-93 76.5


Where results are variable a range has been given


27/31

Quantity per


"CST" May 13

(retained QA sample)


from transport/storage)AA Jan 13

"AA"

Hytech Untreated 93.4 89 78.8

60 Hytech JX 93.5 85.5 36-80 25.5




28/31

Hyola 404RR

Quantity per

treatment (mt)Grower Batches Germ Feb 13 CST Jan 13

"CST" May 13

(retained QA

sample)

Hytech Untreated 96.5 93.4 89

60 Hytech JX 94.5 93.5 85.5

Blend Untreated 97.5 92.5 89

60 Blend MX 95.2




29/31


from

transport/storage)

AA Jan 13

"AA" May 13

(retained QA

sample)

"AA" May 13 (sample

from

transport/storage)

78.8 71.2

36-80 25.5 36.6 5-29

71.1


30/31

Quantity per

treatment (mt)Prod Country Grower Batches CST Jan 13

"CST"

May 13

(retained

QA

sample)

"CST"

May 13

(sample

from

transport/

storage)

Frontier + Hytech Aus + Canada Blend Untreated 92.5 8946.5 + 5.5 Blend MX 90 92 54-93

Canada Hytech Untreated 93.4 89

60 Hytech JX 93.5 85.5 36-80




31/31

AA Jan 13

"AA" May

13

(retained

QA

sample)

"AA" May 13

(sample from

transport/storag

e)

71.176.5 77.6 17-74

78.8 71.2

25.5 36.6 5-29

hyola 404rr consolidated version july 4th 2013

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