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Multidrug-Resistant Microbial Infection and Therapy Study Outbreak of multidrug resistance bacterium infections has become the

major trouble problem among the human being, livestock farm and fish farm. Therapeutic methods by using antibiotics are difficult to control these microbial infections. Multiple drug-resistant bacterium of Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aureus cause serious nosocomial infections in intensive care units in every area of the world. New drugs discovery is more delay speed than the bacterial mutation rate, therefore the new therapeutic processes develop is emergent. The therapeutic methods of phage therapy is an old one, because of the antibiotics resistant of bacterium are increased and the old method is remind again. In our laboratory have prepared many obligate virulent phages for combating these multi-drug resistant bacterium. Besides phage, we also study the lysin proteins, bacteriocin and compound extraction from herbs to investigate the proper agent for treating bacterium infections.

Biomarker Investigation for Osteoarthritis Diagnosis Articular cartilage degeneration is a complex heterogenous disease. The

imbalance in cartilage synthesis and degradation is major to the development of articular cartilage degeneration. The diagnosis of OA is generally base on clinical and radiographic change. Other form of imaging used to measure cartilage tissue directly is MRI (Magnetic resonance imaging), however, the limitation of the radiographs for detecting cartilage destruction is that significant cartilage degradation has occurred. Some biomarkers derived from bone, cartilage and synovium are selected to screen the articular cartilage degenerative progression, but most are major in detecting the specimens from serum or synovial fluid. To avoid collecting the specimens by invasion to

MMMooollleeecccuuulllaaarrr BBBiiiooolllooogggyyy LLLaaabbb... Hung, Chih-Hsin Associate Professor Department of Chemical Engineering & Institute of Biotechnology and Chemical Engineering. I-Shou University Phone: +886-7-6577711 ext 3414 Fax: +886-7-6578945 E-mail: chhung@isu.edu.tw

patients’ bodies, we will develop the rapid detection system to exam the urinary samples from articular cartilage degenerative patients.

IIImmmpppooorrrtttaaannnttt IIInnnssstttrrruuummmeeennntttsss Protein 2-D system equipments (Bio-Rad x1), SDS-PAGE and protein transfer equipments (Bio-Rad x3), Ultra centrifuge (Hitachi x1), PCR machine (x1), agarose gel electroporesis tank (many), Low-temperature and high-speed centrifuge (Kubota 3780x2), High speed centrifuge (Kubota 3300Tx1), CCD digital camera for DNA gel (x1), Autoclave (x3), Sonicator (x1), Incubator (x3), Oven (x1), Chemical hood (x1), Protein purification system (x1), Laminar flow (P2 level) (Length 120 cm x1, 150 cmx1), Whole wave spectrophotometer (x1), 4oC Refrigerator (2 door x2, 3 doorx2), -20 oC Refrigerator (x2), -80 oC Refrigerator (x1), Eletroporator (1 for cell, 1 for bacterium), Dark room (x1)

LLLaaabbb... mmmeeemmmbbbeeerrrsss Ph.D. program: 1 person Graduate student: 3 persons Undergraduate student: 4 persons

SSSeeellleeecccttteeeddd pppaaapppeeerrrsss (((fffrrrooommm 222000000999)))(((SSSCCCIII JJJooouuurrrnnnaaalll))) 1. Wang J. L., C. H. Lai, H. H. Lin, W. F. Chen , Y. C. Shih and C. H. Hung* (2012) Heteroresistant

vancomycin-inter 1 mediate Staphylococcus aureus (hVISA) effect in methicillin-resistant Staphylococcus aureus (MRSA) bacteremia patients with high vancomycin minimum inhibitory concentration. PLoS ONE (In Revised).

2. Shen C. Y., K. N. Huang, S. H. Wang, T. C. Li, Y. C. Chiu, S. M Kuo, S. J. Chang, S. L. Fu, Y. K. Tu, Y. H. Kao and Hung C. H* (2012) Sensitive Quartz Crystal Microbalance Biosensor for Detecting Osteoarthritis by Coating Monoclonal Antibody. Biotechnol. Prog. (In Revised)

3. Liao C. S., C. H. Hung and S. L. Chao, (2012) Decolorization of azo dye reactive black B by Bacillus cereus strain HJ-1. Chemosphere (In Press)

4. Huang K. N., C. Y. Shen, S. H. Wang, C. H. Hung (2012) Development of QCM-based Immunosensor for Detecting Alpha-fetoprotein. Instrument. Sci. & Technol. 44: (In Press).

5. Lin C. C. , C. H. Hung*, C. W. Chang, L. R. Huang (2012), "Rapid Discrimination of Bacteria and Bacteriophages by Raman Spectroscopy", Applied Mechanics and Material. (EI Journal) (accepted)

6. Shen G. H., J. L. Wang, F. S. Wen, K. M. Chang, C. F. Kuo, C. H. Lin, H. R. Luo and C. H. Hung* (2012) Isolation and Characterization of φkm18p, a Novel Lytic Phage with Therapeutic Potential Against Extensively Drug Resistant Acinetobacter baumannii. PLoS ONE 7: e46537..

7. Hung C. H., N Tsao, Y. F. Zeng, S. L. Lu, C. N. Chuan, Y. S. Lin, J. J. Wu and C. F. Kuo. (2012) Synergistic effects of streptolysin S and streptococcal pyrogenic exotoxin B on the mouse model of group A streptococcal infection, Med Microbiol Immunol. 201:357-369.

8. Hung C. H., C. F. Kuo,C.-H. Wang, C. M.Wu, and N. Tsao. (2011) Experimental Phage Therapy in Treating Klebsiella pneumoniae-Mediated Liver Abscesses and Bacteremia in Mice. Antimicrob. Agents Chemother. 55:1358-1365.

9. Wang S. H., C. Y. Shen, T. C. Weng, P. H. Lin, J. J. Yang, I. F. Chen, S. M. Kuo, S. J. Chang, Y. K Tu, Y. H. Kao and C. H. Hung* (2010) Detection of Cartilage Oligomeric Matrix Protein Using a Quartz Crystal Microbalance. Sensors 10:11633-11643.

10. Wang C. P., W. C. Hung, T. H. Yu, C. A. Chiu, L. F. Lu, F. M. Chung, C. H. Hung, S. J. Shin, H. J. Chen, Y. J. Lee. (2010) Genetic variation in the G-50T polymorphism of the cytochrome P450 epoxygenase CYP2J2 gene and the risk of younger onset type 2 diabetes among Chinese population: potential interaction with body mass index and family history. Exp. Clin. Endocrinol. Diabetes 118:346-52.

11. Shen, G. H., C. H. Hung, S. T. Hu, B. D. Wu, C. F. Lin, C. H. Chen, K. M. Wu, and J. H. Chen.(2009) Combining PCR-Restriction Enzyme Analysis with Phenotypic Characters for Mycobacteria Identification in Taiwan. Int. J. Tuberc. Lung Dis. 13:472-479..

12. Shen G. H., C. H. Chen, C. H. Hung, K. M. Wu, Y. Y. Liu, M. Y. Chen, J. H. Chen. (2009)

Combining the Capilia TB assay with smear morphology for rapid identification of Mycobacterium tuberculosis complex. Int. J. Tuberc. Lung Dis.13:371-376.

PPPaaattteeennnttt Nina Tsao, Chih-Hsin Hung, Chih-Feng Kuo "Newly isolated bacteriophage specific to Klebsiella pneumoniae", US 8,252,575 B2,USA. From 2012.08.28 to 2031.01.06. (Updated on 2012 / 11/ 06)

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