humoral and cellular immune response against sars-cov-2
TRANSCRIPT
Humoral and cellular immune response against SARS-CoV-2 variants following heterologous and homologous
Vaccination
Reinhold Förster and Georg Behrensfor the Hannover CoCo-Study group
Analysis scheme
~ 4 month
~ 4 month
~ 4 month ~ 6 month
Parameters determined
• Anti-S1 IgG (Euroimmune; QuantiVac)• Anti-S- IgA• Surrogate virus neutralization test (sVNT)• S-specific B cells
• S-specific CD4 T cells• S-specific CD8 T cells• IGRA
No drop in ISW S-specific B cells
preliminary results
0.0
0.2
0.4
0.6
0.8
% I
SW
S
pik
e+
fro
m C
D19
+C
D2
0+
2 wks post boost
4 mth post boost
pre boost
** ****nsns
Strong drop in S1 antibodies 6 month after boostin BNT/BNT vaccinees
preliminary results
100
200
300
400
500
600
700
800A
nti-S
1 Ig
GR
U/m
l
0
ChAd ChAd ChAd ChAd
- ChAd -
ChAd
ChAd
ChAd
BNT BNT
2 wks 4 mths2 wks 4 mths
BNT BNT BNT
BNT BNT
2 wks 4 mths
BNT
BNT
6 mths
Median: 27.8 162.6 60.5 44.9 626.7 236.4 112.0 648.1 248.9 81.0
EuroImmune SARS-CoV-2 QuantiVac S1
1 RU = 3.2 BAU
-85% compared to 2 wks
Strong drop in S1 antibodies 6 month after boostin BNT/BNT vaccinees
preliminary results
100
200
300
400
500
600
700
800A
nti-S
1 Ig
GR
U/m
l
0
ChAd ChAd ChAd ChAd
- ChAd -
ChAd
ChAd
ChAd
BNT BNT
2 wks 4 mths2 wks 4 mths
BNT BNT BNT
BNT BNT
2 wks 4 mths
BNT
BNT
6 mths
Median: 27.8 162.6 60.5 44.9 626.7 236.4 112.0 648.1 248.9 81.0
EuroImmune SARS-CoV-2 QuantiVac S1
1 RU = 3.2 BAU
-85% compared to 2 wks
Hammerschmidt , re-submittedBosnjak et al, Cell. Mol Immunol; 2020
Surrogate virus neutralization test
Correlation of sVNT with pVNT
De Barros-Martins et al.; Nat. Med.; 2021
Alpha Beta Gamma Delta
Drop in neutralizing antibodies 4 month after boost
preliminary results
Strong drop in neutralizing antibodies 6 month after boostin BNT/BNT vaccinees
preliminary results
Frequencies of Spike-specific CD4+ T cells
preliminary results
Reduced frequencies of Spike-specific CD8+ T cells
preliminary results
Interferon gamma release assay
preliminary results
10
100
1000
10000
100000
IFN
-g m
UI/
ml
ChAd ChAd ChAd ChAd- ChAd -
ChAd
ChAdChAd
BNT BNT
2 wks 4 mths2 wks 4 mths
Summary
• Heterologous immunization with ChAd followed by BNT or MOD induces Spike –specificCD4 and CD8 T cell responses as well as high titers of neutralizing antibodies.
• 4 month after booster immunization a drop in T cell responses is observed with allimmunization protocols.
• ChAd/ChAd vaccinees have lowest Spike-specific T cell counts as well as lowest titers ofall vaccination proocols
• Mod/Mod induces highest titers of Delta neutralizing antibodies
• After six month Delta neutralizing antibodies dropped in BNT/BNT vaccinees byapprox 85%
Joana Barros-MartinsSwantje I. HammerschmidtIvan OdakChristiane RitterMichaela FriedrichsenChristian Schultze-Florey Inga RavensStefanie WillenzonAnja BubkeJasmin RistenpartAnika JanssenGünter BernhardtBerislav Bošnjak
Anne CossmannMetodi V. StankovGema Morillas Ramos Alexandra Dopfer-JablonkaAnnika HeidemannGeorg M. N. Behrens
George SsebyatikaThomas Krey
Markus Hoffmann Stefan Pöhlmann
Jan Münch
Christoph ThurmAnnegret ReinholdDirk Reinhold, Burkhart Schraven2
Acknowledgement
Inst. Immunologie, MHH Klinik für Rheumatologieund Immunology, MHH
Inst. für BiochemieUni Lübeck
Inst. für VirologieUni Ulm
Inst. für ImmunologieUni Magdeburg
Deutsches PrimatenZentrum, Göttingen
