humanized antibody
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Definition
◦Humanized antibodies are antibodies made from non-human species whose protein sequences have been modified to increase their similarity to antibody variants produced naturally in humans.
Human or fully human
A human antibody is one of which both chain types, and the J chain in the case of polymeric antibodies, are of human origin.
Phage display
• The genes for the V regions of antibody heavy and light chains can be inserted separately into these phages, and they display the proteins on the surface.
Gene isolated from;
(a) Naïve B-cells
(b) Immunized, antigen-specific memory B-cells.
Selection The proteins may be selected by
screening against the antigen of choice, and the desirable proteins may be produced and secreted by bacteria infected by the specific phage.
The secreted proteins can be reassembled in vitro to make functional antibodies.
Primarily human systemThe best approach so far for producing
antibody . Strategies used ;
Mouse myeloma cell line transfected with human immunoglobulin genes
Fusion of human B cells with a mouse-human hybrid
'heteromyeloma'