how does sample processing work? insights from a core lab at the university of california, san...

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CTSI Clinical Research Services Clinical & Translational Sciences Institute U C S F This publication made possible by Grant Number UL1 RR024131 from the National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH), and NIH Roadmap for Medical Research. Its contents are solely the responsibility of the authors and do not necessarily represent the official view of NCRR or NIH. Information on NCRR available at http://www.ncrr.nih.gov/. Information on Re-engineering the Clinical Research Enterprise can be obtained from http://nihroadmap.nih.gov/clinicalresearch/ove rview-translational.asp CTSI Clinical Research Services Clinical & Translational Sciences Institute U C S F This publication made possible by Grant Number UL1 RR024131 from the National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH), and NIH Roadmap for Medical Research. Its contents are solely the responsibility of the authors and do not necessarily represent the official view of NCRR or NIH. Information on NCRR available at http://www.ncrr.nih.gov/. Information on Re-engineering the Clinical Research Enterprise can be obtained from http://nihroadmap.nih.gov/clinicalresearch/ove rview-translational.asp The CTSI-CRS Sample Processing Core Laboratories perform specimen processing, sample storage, and shipping for more than 200 clinical studies. Our services are available to all UCSF faculty, residents, and research fellows. We have five laboratories located at: Moffitt Hospital, Adult Moffitt Hospital, Pediatrics Mt. Zion Hospital • SFGH Tenderloin Research Clinic Sample types: • Blood • Urine • Saliva Amniotic Fluid Cerebrospinal Fluid Moffitt Hospital Adult Moffitt Hospital Pediatrics & NCRC Mt. Zion Hospital San Francisco General Hospital Tenderloin Research Center Specimen aliquoting Serum/plasma separation Buffy coat isolation Addition of protease inhibitors, etc. Blood smear preparations Shipping Short-term storage (3 months max.) PBMC isolation EBV transformation of B cells Cryopreservation of cells Cell staining for flow cytometry Point-of-care testing (rapid HIV, rapid HCV) Labeling, Set-up Processing Storage, Inventory Shipping Moffitt Adult Lab SFGH Lab TCRC Lab PCRC staff CTSI-CRS Sample Processing Core Laboratories Wendy Staub, Lab Manager [email protected] 415-794-6460 More than 45,000 samples processed in 2011 Mt. Zion Lab Transfer pipettes

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Page 1: How Does Sample Processing Work? Insights from a Core Lab at the University of California, San Francisco

CTSI Clinical Research Services

Clinical & Translational Sciences Institute

U C S F

This publication made possible by Grant Number UL1 RR024131 from the National Center for Research Resources (NCRR), a

component of the National Institutes of Health (NIH), and NIH Roadmap for Medical Research. Its contents are solely the responsibility of the authors and do not necessarily represent the official view of NCRR or NIH. Information on NCRR available at http://www.ncrr.nih.gov/. Information on Re-engineering the Clinical Research Enterprise can be obtained from

http://nihroadmap.nih.gov/clinicalresearch/overview-translational.asp

CTSI Clinical Research Services

Clinical & Translational Sciences Institute

U C S F

This publication made possible by Grant Number UL1 RR024131 from the National Center for Research Resources (NCRR), a

component of the National Institutes of Health (NIH), and NIH Roadmap for Medical Research. Its contents are solely the responsibility of the authors and do not necessarily represent the official view of NCRR or NIH. Information on NCRR available at http://www.ncrr.nih.gov/. Information on Re-engineering the Clinical Research Enterprise can be obtained from

http://nihroadmap.nih.gov/clinicalresearch/overview-translational.asp

The CTSI-CRS Sample Processing Core Laboratories perform specimen processing, sample storage, and shipping for more than 200 clinical studies. Our services are available to all UCSF faculty, residents, and research fellows. We have five laboratories located at: • Moffitt Hospital, Adult • Moffitt Hospital,

Pediatrics • Mt. Zion Hospital • SFGH • Tenderloin Research

Clinic Sample types: • Blood • Urine • Saliva • Amniotic Fluid • Cerebrospinal Fluid

Moffitt Hospital Adult

Moffitt Hospital

Pediatrics & NCRC

Mt. Zion Hospital

San Francisco General Hospital

Tenderloin Research

Center

Specimen aliquoting

Serum/plasma separation

Buffy coat isolation Addition of protease inhibitors, etc.

Blood smear preparations

Shipping Short-term storage (3 months max.)

PBMC isolation EBV transformation of B cells

Cryopreservation of cells

Cell staining for flow cytometry

Point-of-care testing (rapid HIV, rapid HCV)

Labeling, Set-up

Processing

Storage, Inventory

Shipping

Moffitt Adult Lab SFGH Lab TCRC Lab PCRC staff

CTSI-CRS Sample Processing Core Laboratories Wendy Staub, Lab Manager

[email protected] 415-794-6460

More than 45,000 samples processed in 2011

Mt. Zion Lab Transfer pipettes