JoVE, the Journal of Visualized Experiments, is the first and only peer reviewed PubMed/Medline indexed journal devoted

to the publication of scientific research in a video format. Since 2006 JoVE has been publishing video articles from

laboratories around the world about a variety of scientific disciplines. JoVE also recently introduced Science Education, a

video database to teach laboratory fundamentals. JoVE helps to visualize the complex world of science to increase

educational outcomes, speed learning of lab protocols and methods, and provide a visual basis to accompany traditional

teaching and learning. From basic protocols to specific images and videos that can be used in class, JoVE offers

something for everyone.

The benefits of a JoVE subscription include:

With JoVE, you can provide your school with a 21st century solution for teaching and learning science.

For more information contact your account manager today at libraries@jove.com or call 617-945-9051.

60 new video articles published monthly

Perpetual access to content published during your subscription

Access to archived content

Subscription customized for the sections you require

Guaranteed set price for 3 renewal periods

Unlimited simultaneous users

IP authentication for campus-wide access

Ability to purchase as a monograph, one-time purchase or serials purchase

Authors are provided with a copy of their article for educational, non-commercial use

Scientists can preserve the technique used in their own labs, even after postdocs leave

JoVE helps others understand nuances that may be lost in a print protocol

Techniques can be shared with collaborators without traveling

Authors are able to access viewer statistics not available with traditional journals

Video articles on JoVE get 10,000+ quality views per year

How authors publish in JoVE

Authors write text manuscript using the provided template and submit online. The article will be editorially

and peer reviewed. If accepted, JoVE will script and prepare for filming. Pre-production takes 7-10 weeks.

PRE-PRODUCTION

Our experienced videographers film at the lab. Video can be captured from microscopes, computer

screens, and even underwater. A filming typically takes a full day.

FILMING

After filming, our video team will edit the video, animate schematics, and record voiceovers. The video is

then returned to the author(s) for review. Post production takes 2-3 months.

POST PRODUCTION

Once post production is complete an article is put on the publication calendar and will soon appear on

www.JoVE.com

PUBLISHING

Benefits of publishing in JoVE

JoVE General

JoVE: General is our primary reference resource which includes techniques and protocols from biological, medical,

chemical and physical research. Areas of techniques covered include, but are not limited to:

Developmental Biology

Molecular biology

Bioinformatics

Marine biology

Environmental biology

Veterinary medicine

Non-pathogenic microbiology

Plant Biology

JoVE Neuroscience

JoVE: Neuroscience includes techniques from a wide variety of fields, focusing on those intended to lend insight into the

structure, function, physiology and pathophysiology of the central and peripheral nervous systems. Areas of techniques

covered include, but are not limited to:

Systems neurobiology

Electrophysiology

Development and neural plasticity

Cellular and molecular neurobiology

Animal behavioral testing

Human and animal brain imaging

Psychophysics

Neurobiology of disease

JoVE Immunology and Infection

JoVE: Immunology and Infection includes video articles demonstrating seminal and novel techniques in the fields of

immunology and infectious diseases. The scope of the section includes but is not limited to protocols that address the

following topics:

Basic biology of pathogenic bacteria, fungi, parasites, viruses, and infectious prions

Biology and roles of vectors in microbe life cycles

in vitro and in vivo modeling of pathogenesis

JoVE Journal Sections

JoVE Clinical and Translational Medicine

JoVE: Medicine is a primary reference and educational resource for scientists and physicians. Articles typically document

medical procedures, case reports, clinical trials and translational medicine with direct bedside application. Areas of

techniques covered include:

Animal models of diseases

Surgical subspecialties

Internal medicine

Clinical trials

Behavioral health

Best medical practice

Medical and nursing teaching resources

JoVE Bioengineering

JoVE: Bioengineering focuses on techniques in which the principles of engineering, mathematics and physics

are applied to problems associated with the life sciences. This rapidly growing area of research includes but is

not limited to:

Advanced Instrumentation

Cell and Tissue Engineering

Computational Biology

Synthetic Biology

Bio-MEMS and Microfluidic Devices

Nanotechnology

Biomimetics and Biomaterials

Cybernetics

G

Immune response to infection and tumors

Immune cell development

Autoimmune responses and diseases

Allergic diseases

JoVE Applied Physics

JoVE: Applied Physics focuses on the experimental disciplines within physics, as well as mechanical and electrical

engineering. Interdisciplinary fields such as materials science and chemical physics are welcomed. Instrumentation is

encouraged from all fields especially optics, photonics, and astronomy. The scope of the section includes but is not limited to:

Atomic, molecular, and optical physics

Condensed matter physics

Solid state physics

Plasma physics

Fluid mechanics

Mechanical engineering

Electrical engineering

Materials science and engineering

Organic chemistry

Inorganic chemistry

Physical chemistry

Analytical chemistry

Biochemistry

Chemical biology

Molecular biology

Preparation, use, and analysis of novel materials and structures

Spectroscopic techniques

Molecular self-assembly and recognition

JoVE Chemistry

JoVE: Chemistry focuses on the methodology within chemistry and chemical preparation within multidisciplinary fields.

The chemistry section welcomes articles about new methodologies and new twists on existing methodologies.

The scope of this section includes but is not limited to:

Cognition (Attention, Decision Making, Reasoning)

Sexual and Motivational Behaviors

Social Behaviors

Learning and Memory

Sleep and biological rhythms

Language Processing

JoVE Behavior

JoVE Behavior includes both observational and experimental techniques that seek to understand human and animal behavior,

implicated brain regions, related physiological changes, and underlying genetic causes. The scope of section includes but is

not limited to:

Addiction

Emotion

Control of Movement

Consciousness

Renewable energy and sustainable materials

Environmental engineering

Basic and applied ecology

Ecotoxicology and ecological health

Marine biology and oceanography

Soil and agricultural sciences

Forestry and botany

Atmospheric science and climate modeling

Geosciences

JoVE Environment

JoVE Environment is a multidisciplinary section devoted to the publication of research methods employed in environmental

science and green technologies, from biofuels to oceanography to atmospheric sciences. Special consideration is given to

experimental techniques and approaches aimed at understanding the Earth, protecting natural resources and creating a more

sustainable and environmentally-conscious planet.

The scope of this section includes but is not limited to:

An Introduction to the Centrifuge

Introduction to the Microplate Reader

Understanding Concentration and Measuring Volumes

Making Solutions in the Laboratory

An Introduction to the Micropipettor

Science Education

Science Education Collections

JoVE Science Education is a revolutionary video database dedicated to teaching laboratory fundamentals through video

demonstrations. Each collection features 15 videos showing an overview of a technique or instrument. Every video is paired

with additional video resources to illustrate practical applications and complementary skills.

General Laboratory Techniques

Using a Hemacytometer to Count Cells

Passaging Cells

PCR: The Polymerase Chain Reaction

DNA Gel Electrophoresis

Separating Protein with SDS-PAGE

Bacterial Transformation: The Heat Shock Method

Bacterial Transformation: Electroporation

The ELISA Method

Plasmid Purification

Gel Purification

The Western Blot

An Introduction to Transfection

DNA Ligation Reactions

Restriction Enzyme Digests

Molecular Cloning

Basic Methods in Cellular and Molecular Biology

An Introduction to Saccharomyces cerevisiae

An Introduction to Drosophila melanogaster

An Overview of the Model Organism: C. elegans

Model Organisms I

Introduction to Serological Pipettes and Pipettors

Introduction to the Bunsen Burner

An Introduction to Working in the Hood

Measuring Mass in the Laboratory

Introduction to the Spectrophotometer

Histological Sample Preparation for Light Microscopy

Introduction to Fluorescence Microscopy

Introduction to Light Microscopy

Regulating Temperature in the Lab: Preserving Samples Using Cold

Regulating Temperature in the Lab: Applying Heat

Yeast Maintenance

Yeast Reproduction

Yeast Transformation and Cloning

Isolating Nucleic Acids from Yeast

Drosophila Maintenance and Care

Drosophila Development and Reproduction

Drosophila Embryo Larva

Drosophila Larval IHC

C. elegans Maintenance

Caenorhabditis elegans Development and Reproduction

RNAi in C. elegans

C. elegans Chemotaxis Assay

Novel results compel researchers to pursue unfamiliar,

interdisciplinary experiments.

In the course of scientific discovery, results from one set of

experiments will often necessitate new questions and new, cross

disciplinary experiments. “We had a discovery about five years ago

concerning the role of the circadian system in the development of

the mammary glands and lactation,” Dr. Casey says. The circadian

system governs many cyclical processes in mammalian bodies,

including the sleep/wake cycle and has the reproductive cycles.

These are known commonly as circadian rhythms, and are governed

by the suprachiasmatic nucleus, a group of cells in the

hypothalamus region of the mammalian brain.

“I’ve been doing research for 20 years,

and having JoVE makes things so much easier. JoVE leads to better

results and saves a ton of money, time,

laboratory animals, and frustration!”

Who: Dr. Theresa Casey, Assistant Professor in the Department of

Animal Sciences at Purdue University and member of Dr. Karen

Plaut Lab.

Where: Purdue is a large university in West Lafayette, Indiana with

nearly 40,000 students in attendance each year.

If you are interesting in

adding JoVE as a resource

please contact us by emailing

or by calling

w w w . J o V E . c o m

While searching for methods papers describing how to dissect the suprachiasmatic nucleus in

mice, Dr. Casey and her colleagues discovered JoVE, the world’s first peer reviewed, pub-med

indexed video methods journal. “Once you can see how someone else does something, or see how

someone does something differently than you, it makes your work better,” Dr. Casey explains.

So far, Casey and her colleagues have used JoVE videos to accomplish three different goals in

their laboratory:

Dr. Casey and her colleagues are physiologists, and lacked the clinical neuroscience experience to

properly study the suprachiasmatic nucleus in mice. Other discoveries have caused the Plaut lab to

experiment with different model organisms including mice, rats and cows. Describing the

challenges faced in her lab, Dr. Casey says, “Picking the most appropriate model system is

difficult. In studying the interaction between circadian and mammary systems, we have had to look

in a lot of peripheral tissues.”

Dissection of the Suprachiasmatic Nuclei (SCN) from the hypothalamus – a technique

new to the lab.

Ex Vivo Measurement of Milk Let Down in mice – a new method to measure milk

production in mice.

Differentiation of HC-11 Cells in culture- a familiar technique that visualization helped

match results with previously published literature.

Dr. Casey explains that JoVE has saved the Plaut

Lab thousands of dollars, particularly in travel costs.

“I had a collaborator in Buffalo who knew the SCN

surgery, and I’ve seen it done before. By using the

JoVE video, we saved money in travel costs to go to

Buffalo repeatedly to learn the technique.” By using

Jove to learn the SCN dissection, $6300 was saved

in lost wages; $1100 was saved on travel; and $7700

in reagents. If JoVE helps other labs at Purdue

University save 1/10th of the amount saved by Plaut

Lab, JoVE would save Purdue University’s 100+

labs over $150,000 a year.

Wages $6231 Travel $1100

Reagents $7669

In other ways, JoVE is invaluable and helps make researchers better versed in their colleague’s work.

“I’ve been doing research for 20 years, and having JoVE makes things so much easier. You can

educate yourself on research other scientists are doing around you and get familiarized on a

technique before you try it. I like to watch techniques and refresh myself on experiments I haven’t

conducted in 18 years but need now.” For a seasoned researcher, learning a technique with JoVE is

10x faster than standard journals.

The Plaut lab is also preparing a paper for publication about how they have utilized the HC-11 cell

culture technique that the lab learned from JoVE.

0

10

20

30

40

Da

ys

The time it takes to learn a procedure

Seasoned Researcher

With JoVE (Days)

Without JoVE (Days)

Frequent Student Turnover Reduces Research Productivity.

Each year, as a new class of experienced researchers graduate in

the spring and first year students arrive in the fall, the Drew Lab

faces the reality of academia: student turnover. As new students

work on their own research projects, they turn to Lab Manager Ms.

Jeanette Moore for guidance. “New students will come in and say,

‘oh, you’ve done this experiment in the past, I’m going to take it and

use it here. Who can teach me how?’” Moore explains. When

someone currently at the University is familiar with the technique,

the students are helped immediately. “Often, no one is trained in the

techniques needed. In that case, we would spend up to six months

looking at text references to successfully complete an experiment.”

The problem is compounded by the fact that the Drew Lab works

“With traditional text publications, only descriptions of the experiment were

provided. What was really needed wasfor the experiment

to be shown. Actions teach better than words,

that is where JoVE comes in.”

Who: Jeanette Moore, Research Professional in the Dr. Kelly Drew

Lab at University of Alaska, Fairbanks.

Where: University of Alaska Fairbanks is a public research university

in Fairbanks, Alaska with a student population of 11034.

or by calling

w w w . J o V E . c o m

If you are interesting in

adding JoVE as a resource

please contact us by emailing

It’s important to have the conceptual understanding of what you are doing in an experiment, but as

Ms. Jeanette Moore tells us of text journals, “sometimes you’re reading directions, and the words

just don’t make sense. That is the value of JoVE, actually seeing how to insert a needle in a

laboratory animal before you do it so that you don’t have to do it twice.” JoVE’s peer-reviewed video

articles help Ms. Moore and her students achieve their research goals. While developing a drug

therapy for ischemia, Moore and her students turn to JoVE for instruction and tips for their

experiments. “While we have done blood perfusion and fluorescent immunohistochemistry in the

past, we’ve never done them together while trying to illicit a response to a drug therapy.”

“JoVE makes a difference in our ability to even do these experiments. Scientists are constantly

struggling to find someone to help them, and often not finding anyone. Being in Alaska, and relying

only on the experience of our staff, we’re limited in what we can do.”

JoVE’s ever-expanding library of nearly 2200 videos help Ms. Moore and her colleagues stay

up-to-date with the most efficient techniques to do their experiments and execute complex surgeries

while saving valuable time, money, and animal resources. The students love JoVE because it helps

them graduate on time and with publications they need for their career.

The lab is able to prepare a new paper for publication and has received three new grants thanks

to JoVE’s support.

Using JoVE has saved Ms. Moore and the Drew Lab months of trial and error, saved the laboratory

money, animal resources, and have led to new publications and three new grants. “Instead of trying to

learn a technique from the literature for six months, new researchers can master the method in a few

days. As such, everything is streamlined with JoVE. We use fewer expensive antibodies in our

research, we learn the techniques faster so we use fewer animals, you reduce the drugs you use on

the animals, and you get publishable results. The time saved helps ensure we can push students

through to graduation as well.” The lab saved thousands of dollars on the expensive cFos, GAD67 and

tyrosine hydroxylase. They also applied to NIH grant RO3 because of research achieved with JoVE.

with perfusion in animals. Longer periods of trial and error inevitably mean more animals sacrificed

for the research.

Laboratory techniques established as early as the 1980s are still

valuable in current research; however, few researchers are trained in

these techniques and they can be difficult to learn from traditional

literature.

Drosophila melanogaster, the common fruit fly, is a common animal

model for researchers studying biochemistry, genetics and

neuroscience. Flies have been used for over a century, but rose to

prominence after scientists established techniques to transgenically

manipulate the species genome in the late 1970s and early ‘80s. As

more and more research laboratories use flies, new scientists need

“JoVE videos make researchers become

experts in no time and move on to new experiments.”

Who: Nikolaos Giagtzoglou, Post Doctoral researcher in

neuroscience at Baylor College of Medicine. Currently in the Hugo

J. Bellen lab.

Where: Baylor College of Medicine, located in Houston, Texas, is a

graduate level institution focused on medical and biomedical

research with 1,211 students.

If you are interesting in

adding JoVE as a resource

please contact us by emailing

or by calling

w w w . J o V E . c o m

“I needed to learn three Drosophila techniques: giant fiber recording and dissection in Drosophila,

dissection of ovaries, and backfilling of motor neurons with calcium indicators. I really had no

starting point to learn these techniques, and JoVE was invaluable,” Dr. Giagtzoglou explains. The

worth of these techniques is inestimable when trying to understand adult fly neural structure and

function.

“Even when you meet someone who specializes in a technique, it can be hard to coordinate busy

schedules to travel and learn the method,” Dr. Giagtzoglou tells us. This was the case when he met

Dr. Greg Macleod, a JoVE author and expert in motor neuron backfilling. Dr. Giagtzoglou

discovered Dr. Macleod’s article and immediately saw the benefit of JoVE.

With over 2200 peer-reviewed video-methods articles, Dr. Giagtzoglou found demonstrations of the

techniques he needed and frequently returns to JoVE when learning new techniques or helping

colleagues. “There are at least nine JoVE articles the lab uses regularly, either for review or to learn

a new technique. It’s like night and day. JoVE’s visual demonstration, from the beginning to the

end, is helpful to researchers,” Dr. Giagtzoglou says. “Watching a JoVE video-article is so much

more helpful than reading just materials or methods, which can have grammatical mistakes,

bad syntax, or may be hard to interpret.”

Reduce the time it takes to learn a technique from weeks to days.

Reduce the number of generations of flies required to get experimental results, saving

thousands of dollars in man-hours of researchers and cost of fly upkeep.

Reduce the emotional stress of researchers waiting to get crucial results.

Using JoVE, Dr. Giagtzoglou and his colleagues were able to:

to be trained in laboratory techniques established up to 30 years ago.

Often, these techniques lack recent literature describing their steps, and scientists looking to innovate

need to go to the labs of specialist “gurus” to learn a method. Dr. Nikolaos Giagtzoglou, a

post-doctoral researcher in the Bellen lab at Baylor School of medicine, needed to learn many

techniques to conduct his own research and be a good collaborator. He has found success using

JoVE to learn these new techniques.