Real-Time PCR

Efficiency and flexibility at every step of real-time PCR

High-Throughput Real-Time PCR Solutions

BIO-RAD LABORATORIES, INC. | 32 | BIO-RAD LABORATORIES, INC.

HIGH-THROUGHPUT SOLUTIONS FOR EVERY STEP OF REAL-TIME PCR Bio-Rad’s high-throughput real-time PCR products leverage more than 25 years of expertise. We provide the throughput, flexibility, and reliability required to support drug development from discovery through production.

BIO-RAD LABORATORIES, INC. | 3

PrimePCR™

Gene ExpressionAssays

PrimePCRRun Files

PrimePCR™

Gene ExpressionAssays

PrimePCRRun Files

VS.

GENE TARGET SELECTION

SAMPLE PREP

REACTION SETUP

DATA ACQUISITION

RESULTS

6 STEPS

BIO-RAD WORKFLOW

GENE TARGET SELECTION

ASSAY DESIGN

ASSAY VALIDATION

PLATE DESIGN

SAMPLE PREP

REACTION SETUP

PROTOCOL SELECTION

DATA ACQUISITION

DATA ANALYSISDATA ANALYSIS

RESULTS

10 STEPS

TRADITIONAL WORKFLOW

HIGH THROUGHPUT

MAXIMIZED THROUGHPUT, REDUCED MANUAL INTERVENTIONTraditional real-time PCR workflows include a multitude of unreliable and labor-intensive steps that limit throughput, process efficiency, and scalability. We provide a suite of products that simplify and accelerate real-time PCR to permit true high-throughput gene expression profiling and quantification.

4 | BIO-RAD LABORATORIES, INC.

Bio-Rad Competitor

vs.CFX Automation System II

1CFX Real-Time PCR Detection System

2CFX Real-Time PCR Detection System

43 in. (109 cm)

Twister AutomationRobot

1Competitor Real-Time PCR System

Twister AutomationRobot

2Competitor Real-Time PCR System

97 in. (246 cm)

30 in

. (76

cm

)

3 Complete automated data analysis2 Load run files into automation software

PrimePCR run file

1 Download run files

High-Throughput Plate Handling

The CFX Automation System II is the only robotic microplate handler that can operate two real-time PCR detection systems at the same time without the need for custom software. This true walk-away, high-throughput real-time PCR automation system:

■■ Doubles throughput — 96-well and 384-well real-time PCR detection systems can be paired as required and serviced by a single plate handler

■■ Conserves benchspace — two real-time PCR detection systems can be serviced using a fraction of the footprint of other automation systems

■■ Automates protocol assignment — barcode-specific cycling protocols can be assigned to each plate with or without LIMS integration

HIGH THROUGHPUT

From tissue culture to real-time PCR without time-consuming RNA purification steps. Prepare real-time PCR compatible cell lysates in a single 10 minute step using SingleShot™ Cell Lysis RT-qPCR Kits.

Comprehensive Portfolio of Consumables

Our line of robotics-compatible real-time PCR consumables was designed specifically with automation in mind. Products include:

■■ Temperature-stable supermixes■■ Warp-free Hard-Shell® PCR Plates■■ One-step RT-qPCR kits■■ Kits for RNA purification-independent direct qPCR

Twice the throughput. Half the footprint.The CFX Automation System II can operate two CFX Real-Time PCR Detection Systems at once, doubling throughput and saving valuable benchspace.

From Plates to Analyzed Data in Three Clicks

High-Throughput Automated Solutions for Drug Discovery and Development Our CFX Real-Time PCR Detection Systems, wide range of real-time PCR consumables, and the CFX Automation System II provide streamlined, high-throughput solutions for applications such as:

■■ Target discovery ■■ Target validation■■ Lead screening ■■ Lead optimization■■ Biomarker studies

BIO-RAD LABORATORIES, INC. | 5* U.S. patents 6,627,424; 7,541,170; and 7,560,260

PCR qPCR

NeedsFast Cycling Times

Large Reaction VolumeHigh

Throughput FlexibleStandard

ThroughputLarge

Reaction VolumeHigh

Throughput

Module 96-Well Fast96-Deep Well

384-Well Dual 48/48 Fast CFX96 CFX96 Deep Well CFX384

One thermal cycler chassis, seven reaction modules. The C1000 Touch™ Thermal Cycler Chassis is compatible with four reaction modules for PCR (96–Deep Well, 96-Well Fast, 384-Well, and Dual 48/48 Fast Reaction Modules) and three modules for real-time PCR (CFX96™, CFX96 Deep Well, and CFX384™ Optical Reaction Modules) that accommodate a range of throughput and application requirements.

Interchangeable Module

FLEXIBLE AND CUSTOMIZABLE

Supermixes for All Sample Types

All of our SsoAdvanced™ Supermixes utilize patented* Sso7d fusion polymerase technology, which increases processivity and inhibitor tolerance to ensure superior performance even on challenging templates. Supermixes are available as custom formulations and in custom sizes. Products include:

■■ SsoAdvanced™ Universal SYBR® Green and Probes Supermixes■■ SsoAdvanced™ Universal Inhibitor-Tolerant SYBR® Green Supermix■■ SsoAdvanced PreAmp Supermix■■ SingleShot™ Cell Lysis RT-qPCR Kits

FLEXIBLE AND CUSTOMIZABLE SOLUTIONSReal-time PCR reagent, instrument, and software requirements are as diverse as the products they help bring to the market. To address this diversity in application requirements we have built flexibility and customizability into our entire product line.

Reliable real-time PCR data from even the most challenging samples. SsoAdvanced™ Universal Inhibitor-Tolerant SYBR® Green Supermix is a high-performance real-time PCR supermix that allows generation of superior data using crude lysates from plants, seeds, cells, bacteria, and FFPE samples.

Flexible System Configurations

CFX Real-Time PCR Systems and CFX Manager™ Software provide:

■■ LIMS and automation compatibility■■ Swappable 96- and 384-well reaction modules for real-time PCR■■ Customized run setup and data analysis ■■ Multi-instrument connectivity■■ Two- to five-channel multiplexing■■ CFX Manager Software, Security Edition for U.S. 21 CFR Part 11 compliance■■ Remote notification of run completion with attached data file

Customizable Real-Time PCR Assays and Panels

Bio-Rad’s PrimePCR Gene Expression Assays are expertly designed, and panels are curated to include only biologically relevant genes. PrimePCR Assays are available as:

■■ SYBR® Green– and probe-based individual assays

■■ Custom and predesigned disease and pathway panels

■■ Wet-lab validated and NGS sequence verified assays for the human, rat, and mouse genomes

■■ Expertly in silico designed assays for a range of other model organisms

6 | BIO-RAD LABORATORIES, INC.

RELIABLE PERFORMANCE AND REPRODUCIBLE RESULTSFrom the solid-state optical technology that allows our real-time PCR detection systems to perform reliably run after run and year after year to the algorithms that ensure that all of the most relevant gene targets are represented in each PrimePCR Panel, our real-time PCR portfolio provides confidence in reagents, technology, and results.

CONFIDENCE IN RESULTS

1,000sOF CFX SYSTEMS INSTALLED IN 84COUNTRIES

#1MARKET LEADER IN DIGITAL PCR 1,000sOF Sso7d PUBLICATIONS

15YEARS IN REAL-TIME PCR 27YEARS IN PCR

Expertise

Reliability

Innovation

BIO-RAD LABORATORIES, INC. | 7

On-Site Thermal Validation Services

Our on-site thermal validation service ensures optimal performance of CFX Detection Systems. A Bio-Rad trained and certified service engineer will:

■■ Provide a thermal validation report clearly documenting instrument performance

■■ Assess PCR block accuracy, thermal uniformity, and instrument ramp rate

■■ Confirm thermal block settling times and overshoot are within specifications

On-Site Installation Qualification (IQ) and Operational Qualification (OQ) Services

IQ/OQ services for CFX Detection Systems and the CFX Automation System II are performed on-site. A certified service engineer documents that instruments are installed and functioning properly and ensures that instruments will generate reliable and reproducible data.

CFX Qualification Plate

The CFX Qualification Plate provides a reliable and fast way to qualify any CFX Real-Time PCR Detection System. These sealed and frozen instrument qualification plates contain predispensed reagents and offer:

■■ 1-click pass/fail results■■ Easy integration into QC procedures■■ Convenient troubleshooting

INSTRUMENT VALIDATION AND QUALIFICATIONBio-Rad offers a comprehensive suite of products and services that ensure our real-time PCR instruments perform optimally, experiment after experiment.

4 Thermal Validation Services 4 IQ/OQ

4 Qualification Plates 4 Expert Care Services

Bio-Rad’s Expert Care Service

Bio-Rad’s service and repair plans offer a wide variety of individualized plans.

■■ Choose a preconfigured plan or design your own custom plan for maximum flexibility

■■ Use on-site and off-site services as needed

■■ Consult our team of dedicated experts who have direct access to the latest procedures, parts, tools, and factory-trained engineers to ensure the best service for your instruments

Visit bio-rad.com/support/expertcare for more information.

■■ Extended Cover Plan

■■ Complete Support Plan

■■ Proactive Plan

■■ MyPlan

■■ Repair Plan

■■ Performance Plan

REGULATORY TOOLS AND SERVICES

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Bio-Rad Laboratories, Inc.

Life ScienceGroup

Bio-Rad thermal cyclers and real-time PCR thermal cyclers are covered by one or more of the following U.S. patents or their foreign counterparts owned by Eppendorf AG: U.S. Patent Numbers: 6,767,512 and 7,074,367.

Hard-Shell Plates are covered by one or more of the following U.S. patents or their foreign counterparts owned by Eppendorf AG: U.S. Patent Numbers 7,347,977; 6,340,589; and 6,528,302.

Use of SsoAdvanced Supermixes and PrimePCR PreAmp Assays is covered by one or more of the following U.S. patents and corresponding patent claims outside the U.S.: 5,804,375; 5,994,056; and 6,171,785. The purchase of these products includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser’s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. These product are for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained from the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Purchase of iTaq DNA Polymerase includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser’s own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

SYBR is a trademark of Life Technologies Corporation. Bio-Rad Laboratories, Inc. is licensed by Life Technologies Corporation to sell reagents containing SYBR Green I for use in real-time PCR, for research purposes only.

Twister is a trademark of Zymark Corporation.

Ordering Information

Catalog # Description

1845075 CFX Automation System II for Real-Time PCR Systems, plate handler, barcode scanner, mounting plate and automation software

MSB1001 Microseal® ‘B’ Adhesive Seals, optically clear, 100

HSP3905 Hard-Shell® Thin-Wall 384-Well Skirted PCR Plates, clear shell/white well, 50

HSP9901 Hard-Shell® Low-Profile 96-Well Skirted PCR Plates, white shell/clear well, 50

HSP9955 Hard-Shell® Low-Profile Thin-Wall 96-Well Skirted PCR Plates, white shell/white well, 50

1855195 CFX96 Touch Real-Time PCR Detection System, C1000 Touch Thermal Cycler chassis, CFX96 Optical Reaction Module, CFX Manager Software, qbase+ Software license, cables

1855485 CFX384 Touch Real-Time PCR Detection System, C1000 Touch Thermal Cycler chassis, CFX384 Optical Reaction Module, CFX Manager Software, qbase+ Software license, cables

1854095 CFX96 Touch Deep Well Real-Time PCR Detection System, C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, CFX Manager Software, qbase+ Software license, cables

1814000 PX1 PCR Plate Sealer, heat sealing instrument, plate support block, sealing frame, power cord

1725085 SingleShot SYBR® Green Cell Lysis and RT-qPCR Kit, 500 x 50 µl reactions

1725090 SingleShot SYBR® Green Cell Lysis and RT-qPCR Kit, 100 x 50 µl reactions

1725271 SsoAdvanced™ Universal SYBR® Green Supermix, 5 x 1 ml

1725281 SsoAdvanced™ Universal Probe Supermix, 5 x 1 ml

Visit bio-rad.com/web/qPCRhighthroughput for more information.