high sensitivity rna screentape® eukaryotic rna … · f6 7.5 2.1 99800 28 rna concentration...
TRANSCRIPT
Filename: emilM - HSRNA - 2017-06-07 - 01.HSRNA
Gel Images
Default image (Contrast 50%), Image is Scaled to Sample, Image is Scaled to view larger Molecular Weight range
Default image (Contrast 50%), Image is Scaled to Sample, Image is Scaled to view larger Molecular Weight range
Default image (Contrast 50%), Image is Scaled to Sample, Image is Scaled to view larger Molecular Weight range
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 1 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
Default image (Contrast 50%), Image is Scaled to Sample, Image is Scaled to view larger Molecular Weight range
Sample Info
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsEL1 - - 3750 Electronic Ladder Ladder
A1 2.1 - 190 HS-1001RNA concentrationoutside recommendedrange for RINe
B1 2.1 - 223 HS-1002RNA concentrationoutside recommendedrange for RINe
C1 2.2 - 180 HS-1003RNA concentrationoutside recommendedrange for RINe
D1 2.0 - 118 HS-1004RNA concentrationoutside recommendedrange for RINe
E1 2.1 - 172 HS-1005RNA concentrationoutside recommendedrange for RINe
F1 2.1 - 193 HS-1006RNA concentrationoutside recommendedrange for RINe
G1 2.2 - 209 HS-1007RNA concentrationoutside recommendedrange for RINe
H1 2.2 - 164 HS-1008RNA concentrationoutside recommendedrange for RINe
A2 2.2 - 226 107RNA concentrationoutside recommendedrange for RINe
B2 3.0 - 414 117RNA concentrationoutside recommendedrange for RINe
C2 1.2 - 273 119RNA concentrationoutside recommendedrange for RINe
D2 2.1 - 245 121RNA concentrationoutside recommendedrange for RINe
E2 2.3 - 227 123RNA concentrationoutside recommendedrange for RINe
F2 2.0 - 263 124RNA concentrationoutside recommendedrange for RINe
G2 2.4 - 316 HS-1009RNA concentrationoutside recommendedrange for RINe
H2 2.1 - 271 HS-1010RNA concentrationoutside recommendedrange for RINe
A3 2.1 - 235 69RNA concentrationoutside recommendedrange for RINe
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 2 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
B3 2.1 - 272 70RNA concentrationoutside recommendedrange for RINe
C3 1.9 - 271 71RNA concentrationoutside recommendedrange for RINe
D3 2.0 - 283 74RNA concentrationoutside recommendedrange for RINe
E3 2.2 - 228 86RNA concentrationoutside recommendedrange for RINe
F3 1.5 - 234 88RNA concentrationoutside recommendedrange for RINe
G3 1.9 - 254 91RNA concentrationoutside recommendedrange for RINe
H3 2.8 - 283 106RNA concentrationoutside recommendedrange for RINe
A4 1.8 - 239 52RNA concentrationoutside recommendedrange for RINe
B4 1.6 - 252 53RNA concentrationoutside recommendedrange for RINe
C4 2.6 - 289 59RNA concentrationoutside recommendedrange for RINe
D4 3.7 - 263 60RNA concentrationoutside recommendedrange for RINe
E4 2.3 - 235 62RNA concentrationoutside recommendedrange for RINe
F4 2.2 - 248 63RNA concentrationoutside recommendedrange for RINe
G4 8.1 2.1 8290 67H4 8.8 4.3 1580 68A5 8.6 3.0 1510 38B5 4.4 0.6 1770 39C5 7.9 2.0 8790 42D5 2.3 - 2820 45E5 7.4 2.2 3160 46F5 4.4 0.8 7260 47G5 4.9 1.0 3160 48H5 6.3 2.1 5300 50A6 7.9 1.9 7350 10B6 8.3 2.3 4510 11C6 7.9 2.2 14500 15D6 8.1 2.6 13600 22E6 8.5 2.5 23300 26
F6 7.5 2.1 99800 28RNA concentrationoutside recommendedrange for RINe
G6 6.8 1.6 168000 32RNA concentrationoutside recommendedrange for RINe
H6 4.6 0.5 119000 36RNA concentrationoutside recommendedrange for RINe
A7 8.7 3.1 3870 1B7 8.9 2.6 9200 2
C7 6.9 1.6 78100 5RNA concentrationoutside recommendedrange for RINe
D7 8.7 3.1 1470 7E7 7.7 1.7 24600 73F7 8.0 1.9 24600 77
G7 5.4 0.6 175000 113RNA concentrationoutside recommendedrange for RINe
H7 7.5 2.1 70600 138RNA concentrationoutside recommendedrange for RINe
A8 8.0 2.0 21100 140
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 3 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
B8 7.3 1.8 81300 143RNA concentrationoutside recommendedrange for RINe
C8 7.2 1.8 71800 147RNA concentrationoutside recommendedrange for RINe
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 4 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
EL1: Electronic Ladder
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsEL1 - - 3750 Electronic Ladder Ladder
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker200 189 - 2770 5.98500 638 - 3760 20.251000 557 - 1640 17.662000 580 - 854 18.424000 634 - 466 20.116000 554 - 272 17.58
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 5 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
A1: HS-1001
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
A1 2.1 - 190 HS-1001RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 6 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
B1: HS-1002
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
B1 2.1 - 223 HS-1002RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 7 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
C1: HS-1003
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
C1 2.2 - 180 HS-1003RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 8 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
D1: HS-1004
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
D1 2.0 - 118 HS-1004RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 9 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
E1: HS-1005
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
E1 2.1 - 172 HS-1005RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 10 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
F1: HS-1006
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
F1 2.1 - 193 HS-1006RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 11 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
G1: HS-1007
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
G1 2.2 - 209 HS-1007RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 12 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
H1: HS-1008
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
H1 2.2 - 164 HS-1008RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 13 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
A2: 107
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
A2 2.2 - 226 107RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 14 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
B2: 117
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
B2 3.0 - 414 117RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker882 41.9 - 140 100.00 18S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 15 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
C2: 119
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
C2 1.2 - 273 119RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 16 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
D2: 121
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
D2 2.1 - 245 121RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 17 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
E2: 123
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
E2 2.3 - 227 123RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 18 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
F2: 124
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
F2 2.0 - 263 124RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 19 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
G2: HS-1009
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
G2 2.4 - 316 HS-1009RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker1230 7.15 - 17.1 100.00 18S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 20 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
H2: HS-1010
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
H2 2.1 - 271 HS-1010RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 21 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
A3: 69
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
A3 2.1 - 235 69RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 22 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
B3: 70
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
B3 2.1 - 272 70RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 23 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
C3: 71
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
C3 1.9 - 271 71RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker661 16.6 - 73.8 100.00 18S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 24 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
D3: 74
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
D3 2.0 - 283 74RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 25 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
E3: 86
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
E3 2.2 - 228 86RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 26 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
F3: 88
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
F3 1.5 - 234 88RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker1160 7.36 - 18.7 100.00 18S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 27 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
G3: 91
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
G3 1.9 - 254 91RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 28 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
H3: 106
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
H3 2.8 - 283 106RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker804 22.3 - 81.6 100.00 18S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 29 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
A4: 52
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
A4 1.8 - 239 52RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 30 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
B4: 53
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
B4 1.6 - 252 53RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker1252 8.59 - 20.2 100.00 18S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 31 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
C4: 59
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
C4 2.6 - 289 59RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker863 7.02 - 23.9 100.00 18S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 32 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
D4: 60
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
D4 3.7 - 263 60RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker887 24.4 - 81.0 100.00 18S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 33 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
E4: 62
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
E4 2.3 - 235 62RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker1384 4.80 - 10.2 100.00 18S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 34 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
F4: 63
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
F4 2.2 - 248 63RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 35 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
G4: 67
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsG4 8.1 2.1 8290 67
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker1247 1630 - 3840 32.63 18S3248 3360 - 3040 67.37 28S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 36 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
H4: 68
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsH4 8.8 4.3 1580 68
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker1252 234 - 549 18.86 18S3769 1010 - 785 81.14 28S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 37 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
A5: 38
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsA5 8.6 3.0 1510 38
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker1237 222 - 528 25.24 18S3878 657 - 498 74.76 28S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 38 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
B5: 39
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsB5 4.4 0.6 1770 39
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker1153 257 - 655 62.86 18S3543 152 - 126 37.14 28S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 39 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
C5: 42
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsC5 7.9 2.0 8790 42
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker1131 1680 - 4380 33.05 18S2957 3410 - 3390 66.95 28S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 40 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
D5: 45
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsD5 2.3 - 2820 45
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker791 181 - 673 100.00 18S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 41 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
E5: 46
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsE5 7.4 2.2 3160 46
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker1206 497 - 1210 30.85 18S3384 1120 - 969 69.15 28S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 42 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
F5: 47
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsF5 4.4 0.8 7260 47
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker881 1560 - 5220 55.96 18S2295 1230 - 1580 44.04 28S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 43 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
G5: 48
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsG5 4.9 1.0 3160 48
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker1204 463 - 1130 50.05 18S3416 462 - 398 49.95 28S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 44 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
H5: 50
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsH5 6.3 2.1 5300 50
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker1214 644 - 1560 32.04 18S3262 1370 - 1230 67.96 28S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 45 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
A6: 10
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsA6 7.9 1.9 7350 10
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker1186 1520 - 3770 34.77 18S3047 2850 - 2750 65.23 28S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 46 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
B6: 11
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsB6 8.3 2.3 4510 11
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker1191 802 - 1980 30.48 18S3206 1830 - 1680 69.52 28S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 47 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
C6: 15
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsC6 7.9 2.2 14500 15
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker1174 2720 - 6830 31.56 18S2904 5910 - 5980 68.44 28S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 48 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
D6: 22
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsD6 8.1 2.6 13600 22
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker1144 2290 - 5880 27.84 18S2861 5920 - 6090 72.16 28S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 49 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
E6: 26
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsE6 8.5 2.5 23300 26
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker880 4170 - 14000 28.50 18S2137 10500 - 14400 71.50 28S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 50 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
F6: 28
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
F6 7.5 2.1 99800 28RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker790 20300 - 75400 32.14 18S1807 42800 - 69600 67.86 28S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 51 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
G6: 32
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
G6 6.8 1.6 168000 32RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker857 38500 - 132000 37.74 18S1982 63500 - 94200 62.26 28S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 52 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
H6: 36
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
H6 4.6 0.5 119000 36RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker1657 25100 - 44600 64.86 18S1886 13600 - 21200 35.14 28S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 53 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
A7: 1
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsA7 8.7 3.1 3870 1
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker1183 554 - 1380 24.63 18S3219 1700 - 1550 75.37 28S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 54 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
B7: 2
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsB7 8.9 2.6 9200 2
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker1170 1670 - 4190 27.54 18S2976 4380 - 4330 72.46 28S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 55 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
C7: 5
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
C7 6.9 1.6 78100 5RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker822 18400 - 66000 38.02 18S1936 30000 - 45700 61.98 28S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 56 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
D7: 7
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsD7 8.7 3.1 1470 7
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker1150 213 - 544 24.36 18S3563 660 - 545 75.64 28S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 57 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
E7: 73
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsE7 7.7 1.7 24600 73
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker771 5710 - 21800 36.80 18S1851 9800 - 15600 63.20 28S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 58 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
F7: 77
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsF7 8.0 1.9 24600 77
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker769 5590 - 21400 34.59 18S1855 10600 - 16800 65.41 28S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 59 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
G7: 113
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
G7 5.4 0.6 175000 113RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker1822 40600 - 65600 62.28 18S2057 24600 - 35200 37.72 28S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 60 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
H7: 138
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
H7 7.5 2.1 70600 138RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker814 14400 - 51900 32.09 18S1888 30400 - 47400 67.91 28S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 61 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
A8: 140
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsA8 8.0 2.0 21100 140
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker1142 4440 - 11400 33.09 18S2812 8980 - 9390 66.91 28S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 62 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
B8: 143
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
B8 7.3 1.8 81300 143RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker760 18200 - 70300 36.08 18S1772 32200 - 53400 63.92 28S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 63 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017
C8: 147
Sample Table
Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations
C8 7.2 1.8 71800 147RNA concentrationoutside recommendedrange for RINe
Peak Table
Size [nt] Calibrated Conc.[pg/µl]
Assigned Conc.[pg/µl]
Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations
25 700 700 82400 - Lower Marker807 16200 - 59000 35.55 18S1852 29300 - 46600 64.45 28S
High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 64 of 64
TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017