heterogeneous nutrient sources create niche ...algaebiomass.org/wp-content/gallery/2012-algae... ·...
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ORNL is managed by UT-Battelle for the US Department of Energy
Heterogeneous nutrient sources create niche differentiation for algal polycultures
Teresa Mathews Oak Ridge National Laboratory [email protected]
Shovon Mandal Jonathan Shurin
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Goals & Challenges
• 2500-5000 gallons biofuel intermediate/acre/y at a cost of $3 GGE
• Challenges – Cost: need ~5x reduction in cost – Scale: crop losses & natural resources limiting
**Cost-effective nutrient & water sources and crop protection are key to large scale sustainable algal biomass production
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Algal biomass production: Monoculture vs polyculture
• In nature, algae exist as polycultures
• Biofuel industry focus is on monoculture strains selected for high oil, growth rates
• Grow well under controlled laboratory conditions
Most research has focused on cellular level processes
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The problem with monocultures
Treated
Untreated
Many of the problems associated with scaling up are ecological
Fungi Virus
Aquatic systems ~5x more susceptible to pests than terrestrial crops
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Polycultures for crop protection
Monoculture
2 spp polyculture
Corcoran & Boeing, 2012
Polycultures with different sized cells suppressed grazing
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Can polycultures outperform monoculture productivity?
Shurin et al., 2014
Overyielding can occur if polycultures include species with complementary traits or that occupy different niches
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Creating niche differentiation with nutrients
• 106 C: 16 N: 1 P – Nitrate (NO3) – Ammonia (NH4) – Urea (NH2)2CO
• How do algal growth rates differ when given different nitrogen sources?
• Do different algal species prefer different nitrogen sources?
• Can polycultures grown in mixed nutrient media outperform monocultures?
Culture media
Waste water
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Selected algae
Cyanobacteria (Blue green algae) 13. Synechococcous sp.
Cyanelles (Glaucophytes) 12. Cyanophora biloba
Heterokonts(Diatoms) 1. Navicula sp. 2. Cyclotella menenghiniana Chlorophytes (Green Algae) 3. Raphidocelis subcapitata 4. Chlamydomonas reinhardtii UTEX 2243 5. Chlamydomonas reinhardtii 6. Scenedesmus obliquus 7. Scenedesmus dimorphus 8. Neochloris oleabundans 9. Chlorococcoum oleofaciens 10. Tetraselmis sp. 11. Chlorella vulgaris
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Selected algae
Cyanobacteria (Blue green algae) 13. Synechococcous sp.
Cyanelles (Glaucophytes) 12. Cyanophora biloba
Heterokonts(Diatoms) 1. Navicula sp. 2. Cyclotella menenghiniana Chlorophytes (Green Algae) 3. Raphidocelis subcapitata 4. Chlamydomonas reinhardtii UTEX 2243 5. Chlamydomonas reinhardtii 6. Scenedesmus obliquus 7. Scenedesmus dimorphus 8. Neochloris oleabundans 9. Chlorococcoum oleofaciens 10. Tetraselmis sp. 11. Chlorella vulgaris
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Experimental design
Cyanobacteria (Blue green algae) 13. Synechococcous sp.
Cyanelles (Glaucophytes) 12. Cyanophora biloba
Heterokonts(Diatoms) 1. Navicula sp. 2. Cyclotella menenghiniana Chlorophytes (Green Algae) 3. Raphidocelis subcapitata 4. Chlamydomonas reinhardtii 5. Chlamydomonas reinhardtii 6. Scenedesmus obliquus 7. Scenedesmus dimorphus 8. Neochloris oleabundans 9. Chlorococcoum oleofaciens 10. Tetraselmis sp. 11. Chlorella vulgaris
NO3 media (WC media)
NH4 media
Urea media
Simulated waste water media
Select complementary
strains
Equimolar nitrogen in each culture medium
Equimolar amounts of each nitrogen species
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Preliminary results
Ammonium Nitrate Urea
Cyanobacteria Glaucophyte
Diatoms
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Preliminary results
Ammonium Nitrate Urea
Green algae
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Preliminary conclusions
Ammonium Nitrate Urea
Green algae
Up to 3 fold differences in growth rates in some species with different N sources
Optimal N source different between different species
Nitrate was not always the optimal N source; cost reduction!
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Next steps
• Polycultures in homogeneous & mixed media (2 & 5 species combos)
• Quantify relative yield (productivity), nutrient use efficiency, stability
• Add stressors (grazers, chytrid fungi) to polycultures in mixed and homogeneous media
Polycultures are a reality of large scale algal production. Understanding and managing species interactions could significantly help the biofuel industry.
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Questions?
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