hepatitis b serology laboratory : distribution : 4202 page ... · specimen 4140: 519 iu/ml (16-946)...

14
Dispatch Date : 01-Jan-2018 Page 1 Hepatitis B serology Distribution : 4202 Laboratory : Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG © Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:18 on Thursday 12 July 2018 Your Score Intended Result Your Report Specimen 4138 HBsAg positive HBsAg positive 2 Anti-HBc positive Anti-HBc positive 2 Anti-HBc IgM negative Anti-HBc IgM negative 2 HBeAg negative HBeAg negative 2 Anti-HBe positive Anti-HBe positive 2 Specimen 4139 HBsAg positive HBsAg positive 2 Anti-HBc positive Anti-HBc positive 2 Anti-HBc IgM positive Anti-HBc IgM positive 2 HBeAg negative HBeAg negative 2 Anti-HBe positive Anti-HBe positive Not scored Specimen 4140 HBsAg positive HBsAg positive 2 Anti-HBc positive Anti-HBc positive 2 Anti-HBc IgM negative Anti-HBc IgM negative 2 HBeAg negative HBeAg negative 2 Anti-HBe positive Anti-HBe positive 2 Specimen 4141 HBsAg negative HBsAg negative 2 Anti-HBc negative Anti-HBc negative 2 Anti-HBc IgM negative Anti-HBc IgM negative 2 HBeAg negative HBeAg negative 2 Anti-HBe negative Anti-HBe negative 2 Specimen 4142 HBsAg positive HBsAg positive 2 Anti-HBc positive Anti-HBc positive 2 Anti-HBc IgM negative Anti-HBc IgM negative 2 HBeAg negative HBeAg negative 2 Anti-HBe positive Anti-HBe positive 2 Specimen 4143 HBsAg negative HBsAg negative 2 Anti-HBc negative Anti-HBc negative 2 Anti-HBc IgM negative Anti-HBc IgM negative 2 HBeAg negative HBeAg negative 2 Anti-HBe negative Anti-HBe negative 2 of 14

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Page 1: Hepatitis B serology Laboratory : Distribution : 4202 Page ... · Specimen 4140: 519 IU/mL (16-946) with Abbott Architect; 340 IU/mL (22-1000) with DiaSorin Liaison. Specimen 4142:

Dispatch Date : 01-Jan-2018

Page 1

Hepatitis B serology

Distribution : 4202

Laboratory :

Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG

© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:18 on Thursday 12 July 2018

Your Score Intended Result Your Report

Specimen 4138 HBsAg positive HBsAg positive 2 Anti-HBc positive Anti-HBc positive 2 Anti-HBc IgM negative Anti-HBc IgM negative 2 HBeAg negative HBeAg negative 2 Anti-HBe positive Anti-HBe positive 2

Specimen 4139 HBsAg positive HBsAg positive 2 Anti-HBc positive Anti-HBc positive 2 Anti-HBc IgM positive Anti-HBc IgM positive 2 HBeAg negative HBeAg negative 2 Anti-HBe positive Anti-HBe positive Not scored

Specimen 4140 HBsAg positive HBsAg positive 2 Anti-HBc positive Anti-HBc positive 2 Anti-HBc IgM negative Anti-HBc IgM negative 2 HBeAg negative HBeAg negative 2 Anti-HBe positive Anti-HBe positive 2

Specimen 4141 HBsAg negative HBsAg negative 2 Anti-HBc negative Anti-HBc negative 2 Anti-HBc IgM negative Anti-HBc IgM negative 2 HBeAg negative HBeAg negative 2 Anti-HBe negative Anti-HBe negative 2

Specimen 4142 HBsAg positive HBsAg positive 2 Anti-HBc positive Anti-HBc positive 2 Anti-HBc IgM negative Anti-HBc IgM negative 2 HBeAg negative HBeAg negative 2 Anti-HBe positive Anti-HBe positive 2

Specimen 4143 HBsAg negative HBsAg negative 2 Anti-HBc negative Anti-HBc negative 2 Anti-HBc IgM negative Anti-HBc IgM negative 2 HBeAg negative HBeAg negative 2 Anti-HBe negative Anti-HBe negative 2

of 14

Page 2: Hepatitis B serology Laboratory : Distribution : 4202 Page ... · Specimen 4140: 519 IU/mL (16-946) with Abbott Architect; 340 IU/mL (22-1000) with DiaSorin Liaison. Specimen 4142:

Dispatch Date : 01-Jan-2018

Page 2

Hepatitis B serology

Distribution : 4202

Laboratory :

Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG

© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:18 on Thursday 12 July 2018

Cumulative score information Total number of specimens sent to you for UK NEQAS for Hepatitis B serology over the last 3 distributions is 18 For these distributions specimen numbers 3789 3790 3791 3792 3793 3794 3956 3957 3958 3959 3960 3961 4138 4139 4140 4141 4142 4143 have been sent. Number of specimens reported too late for analysis (not scored) 0 Your cumulative score for the specimen/test combinations that you reported was 174 out of a possible total of 174 The mean score calculated from the reports returned by UK laboratories testing the specimen/test combinations you examined was 171.86 (with a standard error of 2.71). Performance rating Your performance rating for UK NEQAS for Hepatitis B serology (i.e. the number of standard errors by which your cumulative score lies above or below the mean for UK laboratories) is 0.79. A performance rating of more than 1.96 standard errors below the mean indicates possible poor performance. Your performance rating may change if other participants' results are amended. No score penalty is incurred for non return of reports. However non reporting of results may be used as a measure of poor performance.

Comments: Overall performance was excellent with 99.7% of participants reporting correctly for HBsAg, 99.9% for anti-HBc, 99.7% for anti-HBc IgM, 99.6% for HBeAg and 97.9% for anti-HBe. Comment on specimen 4139 Anti-HBe: 169/259 (65.3%) of participants reported a positive anti-HBe result for this specimen, 54/259 (20.8%) reported a negative result and 36/259 (13.9%) reported an equivocal result. Therefore consensus for this specimen was not reached and is not scored. Pre-distribution testing showed a positive anti-HBe result using the Abbott Architect and Biomerieux Vidas platforms with a negative anti-HBe result using the Diasorin ETI-EBK PLUS EIA. Cumulative performance information is presented on this page. In the histograms on page 3 and subsequent pages a maximum of 12 kit results are displayed: these include the most commonly used methods and the method(s) used in your laboratory is indicated by an arrow. The figures in the histograms and those in the overall results tables may differ (1) due to exclusion of kits displayed in the histograms resulting in apparently lower numbers of data sets in the histograms or (2) due to participants using more than one kit resulting in higher numbers of data sets in the histograms. HBsAg quantitative results : 45 participants reported quantitative results expressed in IU/mL using the Abbott Architect quantitative assay (n=14) and the DiaSorin Liaison assay (n=31). Median (lowest-highest) values by assay were as follows: Specimen 4138: 527 IU/mL (18-943) with Abbott Architect; 332 IU/mL (20-1000) with DiaSorin Liaison. Specimen 4139: 6 IU/mL (0-11) with Abbott Architect; 4 IU/mL (0-14) with DiaSorin Liaison. Specimen 4140: 519 IU/mL (16-946) with Abbott Architect; 340 IU/mL (22-1000) with DiaSorin Liaison. Specimen 4142: 4402 IU/mL (250-7751) with Abbott Architect; 3450 IU/mL (150-9643) with DiaSorin Liaison. Specimens 4141 and 4143 were negative for all markers. The quantitative results provided by the participants varied significantly to each other and were also different to the pre- distribution testing results that we have reported. If you use a quantitative assay please specify your method and report your results so we can include them in the analysis. Turn around time: The time taken to report your results was 4-days. This information is provided for your own use and does not form part of your performance assessment. Acknowledgements : We thank colleagues at PHE-NIS-Public Health Laboratory, Southampton; PHE-NIS-Virus Reference Division, Colindale; PHE-NIS-Public Health Laboratory, Manchester; Gloucestershire Royal Hospital; and NHSBT, Colindale for their kind assistance with pre-distribution testing. Enquiries: Repeat specimens can be ordered using the web form or by emailing [email protected]. stating your laboratory number, distribution number and type, and specimen number(s). Pre-distribution test results are available should you experience a technical failure and wish to discuss the results. Any technical queries about this distribution should be emailed to Habib Seyedzadeh at the above address. Report authorised by: Dr Sanjiv Rughooputh, Director

Per

form

ance

Rat

ing

Distribution

Your performance rating over the past 12 distributions Your current performance rating is 0.79

MICPERF2

3467 3517 3572 3677 3729 3787 3901 3954 4013 4086 4141 4202-4

-3

-2

-1

0

1

2A

llU

K

Tot

al s

core

Distribution

Total score you achieved for each of the last 12 distributionsYour total score for this distribution is 58

MICPERF2X

3467 3517 3572 3677 3729 3787 3901 3954 4013 4086 4141 42020

20

40

60

80

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Page 3: Hepatitis B serology Laboratory : Distribution : 4202 Page ... · Specimen 4140: 519 IU/mL (16-946) with Abbott Architect; 340 IU/mL (22-1000) with DiaSorin Liaison. Specimen 4142:

Dispatch Date : 01-Jan-2018

Page 3

Hepatitis B serology

Distribution : 4202

Laboratory :

Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG

© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:18 on Thursday 12 July 2018

Six specimens were dispatched with the request to report on hepatitis B surface antigen (HBsAg) status and other hepatitis B markers. The specimens were derived from thrombinised human plasma (serum) donations. Specimens 4138, 4139, 4140 and 4142 were diluted 1:9.7, 1:4, 1:242, 1:6.9 respectively in negative thrombinised human plasma (serum). Specimens 4141 and 4143 were pools of negative thrombinised human plasma (serum).

Specimen : 4138 HBsAg positive (26 IU/mL)

UK (%) All (%)

8 (3.5) 9 (1.6)Biokit : Bioelisa60 (26.1) 76 (13.5)bioMerieux : Vidas49 (21.3) 178 (31.6)Architect HBsAg qual II

7 (3.0) 8 (1.4)Beckman : Access3 (1.3) 11 (2.0)Abbott : Architect5 (2.2) 14 (2.5)BioRad : Monolisa7 (3.0) 16 (2.8)Murex2 (0.9) 17 (3.0)Architect HBsAg quant

10 (4.3) 21 (3.7)Neutralisation (all kits)10 (4.3) 27 (4.8)DiaSorin : Liaison14 (6.1) 35 (6.2)Siemens:ADVIA Centaur47 (20.4) 108 (19.1)Roche

Number of Reports0 40 80 120 160 200

Positive Equivocal Negative

Your Report : HBsAg positive

HBsAg equivocal

140

HBsAg positive

0

Overall Results

388

-1

% Correct

00

100

------------------------------------------------------------UK

3880

Total

1HBsAg negative

140

All

100

------------------------------------------------------------

2

Score

Your Score : 2

Specimen : 4138 HBsAg positive (26 IU/mL) Anti-HBc positive

UK (%) All (%)

5 (3.3) 6 (1.5)Biokit : Bioelisa47 (30.7) 169 (42.5)Architect anti-HBc II

2 (1.3) 4 (1.0)DiaSorin0 (0.0) 4 (1.0)Abbott : Prism0 (0.0) 5 (1.3)OCD : VITROS6 (3.9) 7 (1.8)Beckman : Access1 (0.7) 8 (2.0)BioRad : Monolisa1 (0.7) 10 (2.5)Abbott : Architect7 (4.6) 19 (4.8)DiaSorin : Liaison9 (5.9) 28 (7.0)Siemens:ADVIA Centaur

34 (22.2) 44 (11.1)bioMerieux : Vidas39 (25.5) 80 (20.1)Roche

Number of Reports0 40 80 120 160 200

Positive Equivocal Negative

Your Report : Anti-HBc positive

Anti-HBc equivocal

129

Anti-HBc positive

0

Overall Results

347

-1

% Correct

00

100

------------------------------------------------------------UK

3470

Total

1Anti-HBc negative

129

All

100

------------------------------------------------------------

2

Score

Your Score : 2

Specimen : 4138 HBsAg positive (26 IU/mL) Anti-HBc IgM negative

UK (%) All (%)

30 (41.7) 121 (50.2)Abbott : Architect1 (1.4) 1 (0.4)BioRad : Monolisa0 (0.0) 1 (0.4)Beckman0 (0.0) 1 (0.4)DiaSorin0 (0.0) 2 (0.8)Dia.Pro1 (1.4) 2 (0.8)Beckman : Access0 (0.0) 2 (0.8)Unspecified0 (0.0) 6 (2.5)OCD : VITROS3 (4.2) 13 (5.4)DiaSorin : Liaison3 (4.2) 18 (7.5)Siemens:ADVIA Centaur

24 (33.3) 28 (11.6)bioMerieux : Vidas10 (13.9) 46 (19.1)Roche

Number of Reports0 30 60 90 120 150

Positive Equivocal Negative

Your Report : Anti-HBc IgM negative

Anti-HBc IgM equivocal

73

Anti-HBc IgM positive

244

Overall Results

244

2

% Correct

730

100

------------------------------------------------------------UK

00

Total

1Anti-HBc IgM negative

0

All

100

------------------------------------------------------------

-1

Score

Your Score : 2

Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG

© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:18 on Thursday 12 July 2018

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Page 4: Hepatitis B serology Laboratory : Distribution : 4202 Page ... · Specimen 4140: 519 IU/mL (16-946) with Abbott Architect; 340 IU/mL (22-1000) with DiaSorin Liaison. Specimen 4142:

Dispatch Date : 01-Jan-2018

Page 4

Hepatitis B serology

Distribution : 4202

Laboratory :

Six specimens were dispatched with the request to report on hepatitis B surface antigen (HBsAg) status and other hepatitis B markers. The specimens were derived from thrombinised human plasma (serum) donations. Specimens 4138, 4139, 4140 and 4142 were diluted 1:9.7, 1:4, 1:242, 1:6.9 respectively in negative thrombinised human plasma (serum). Specimens 4141 and 4143 were pools of negative thrombinised human plasma (serum).

Specimen : 4138 HBsAg positive (26 IU/mL) HBeAg negative

UK (%) All (%)

24 (31.6) 28 (10.6)bioMerieux : Vidas31 (40.8) 137 (51.7)Abbott : Architect

0 (0.0) 1 (0.4)Dia.Pro0 (0.0) 2 (0.8)BioRad : Monolisa0 (0.0) 2 (0.8)Unspecified0 (0.0) 4 (1.5)OCD : VITROS1 (1.3) 4 (1.5)DiaSorin3 (3.9) 16 (6.0)DiaSorin : Liaison3 (3.9) 19 (7.2)Siemens:ADVIA Centaur

14 (18.4) 52 (19.6)Roche

Number of Reports0 30 60 90 120 150

Positive Equivocal Negative

Your Report : HBeAg negative

HBeAg equivocal

76

HBeAg positive

259

Overall Results

261

2

% Correct

751

99.2

------------------------------------------------------------UK

10

Total

1HBeAg negative

1

All

98.7

------------------------------------------------------------

-1

Score

Your Score : 2

Specimen : 4138 HBsAg positive (26 IU/mL) Anti-HBe positive

UK (%) All (%)

24 (31.6) 26 (10.0)bioMerieux : Vidas31 (40.8) 136 (52.1)Abbott : Architect

0 (0.0) 1 (0.4)Dia.Pro0 (0.0) 1 (0.4)Ortho0 (0.0) 1 (0.4)BioRad : Monolisa0 (0.0) 1 (0.4)Biokit : Bioelisa0 (0.0) 2 (0.8)Unspecified0 (0.0) 3 (1.1)OCD : VITROS1 (1.3) 4 (1.5)DiaSorin3 (3.9) 16 (6.1)DiaSorin : Liaison3 (3.9) 19 (7.3)Siemens:ADVIA Centaur

14 (18.4) 51 (19.5)Roche

Number of Reports0 30 60 90 120 150

Positive Equivocal Negative

Your Report : Anti-HBe positive

Anti-HBe equivocal

76

Anti-HBe positive

1

Overall Results

259

-1

% Correct

01

99.2

------------------------------------------------------------UK

2570

Total

1Anti-HBe negative

76

All

100

------------------------------------------------------------

2

Score

Your Score : 2

Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG

© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:18 on Thursday 12 July 2018

of 14

Page 5: Hepatitis B serology Laboratory : Distribution : 4202 Page ... · Specimen 4140: 519 IU/mL (16-946) with Abbott Architect; 340 IU/mL (22-1000) with DiaSorin Liaison. Specimen 4142:

Dispatch Date : 01-Jan-2018

Page 5

Hepatitis B serology

Distribution : 4202

Laboratory :

Six specimens were dispatched with the request to report on hepatitis B surface antigen (HBsAg) status and other hepatitis B markers. The specimens were derived from thrombinised human plasma (serum) donations. Specimens 4138, 4139, 4140 and 4142 were diluted 1:9.7, 1:4, 1:242, 1:6.9 respectively in negative thrombinised human plasma (serum). Specimens 4141 and 4143 were pools of negative thrombinised human plasma (serum).

Specimen : 4139 HBsAg positive (0.3 IU/mL)

UK (%) All (%)

8 (3.5) 9 (1.6)Biokit : Bioelisa60 (26.4) 77 (13.7)bioMerieux : Vidas49 (21.6) 179 (31.7)Architect HBsAg qual II

7 (3.1) 8 (1.4)Beckman : Access3 (1.3) 11 (2.0)Abbott : Architect5 (2.2) 14 (2.5)BioRad : Monolisa7 (3.1) 17 (3.0)Murex2 (0.9) 17 (3.0)Architect HBsAg quant9 (4.0) 20 (3.5)Neutralisation (all kits)

11 (4.8) 29 (5.1)DiaSorin : Liaison14 (6.2) 35 (6.2)Siemens:ADVIA Centaur46 (20.3) 107 (19.0)Roche

Number of Reports0 40 80 120 160 200

Positive Equivocal Negative

Your Report : HBsAg positive

HBsAg equivocal

140

HBsAg positive

0

Overall Results

388

-1

% Correct

02

99.5

------------------------------------------------------------UK

3861

Total

1HBsAg negative

139

All

99.3

------------------------------------------------------------

2

Score

Your Score : 2

Specimen : 4139 HBsAg positive (0.3 IU/mL) Anti-HBc positive

UK (%) All (%)

5 (3.2) 6 (1.5)Biokit : Bioelisa47 (30.3) 169 (42.0)Architect anti-HBc II

2 (1.3) 4 (1.0)DiaSorin0 (0.0) 4 (1.0)Abbott : Prism0 (0.0) 5 (1.2)OCD : VITROS6 (3.9) 7 (1.7)Beckman : Access1 (0.6) 8 (2.0)BioRad : Monolisa1 (0.6) 11 (2.7)Abbott : Architect7 (4.5) 19 (4.7)DiaSorin : Liaison9 (5.8) 28 (7.0)Siemens:ADVIA Centaur

35 (22.6) 45 (11.2)bioMerieux : Vidas40 (25.8) 81 (20.1)Roche

Number of Reports0 40 80 120 160 200

Positive Equivocal Negative

Your Report : Anti-HBc positive

Anti-HBc equivocal

130

Anti-HBc positive

0

Overall Results

348

-1

% Correct

00

100

------------------------------------------------------------UK

3480

Total

1Anti-HBc negative

130

All

100

------------------------------------------------------------

2

Score

Your Score : 2

Specimen : 4139 HBsAg positive (0.3 IU/mL) Anti-HBc IgM positive

UK (%) All (%)

30 (41.7) 121 (50.2)Abbott : Architect1 (1.4) 1 (0.4)BioRad : Monolisa0 (0.0) 1 (0.4)Beckman0 (0.0) 1 (0.4)DiaSorin0 (0.0) 2 (0.8)Dia.Pro1 (1.4) 2 (0.8)Beckman : Access0 (0.0) 2 (0.8)Unspecified0 (0.0) 6 (2.5)OCD : VITROS3 (4.2) 13 (5.4)DiaSorin : Liaison3 (4.2) 18 (7.5)Siemens:ADVIA Centaur

24 (33.3) 28 (11.6)bioMerieux : Vidas10 (13.9) 46 (19.1)Roche

Number of Reports0 30 60 90 120 150

Positive Equivocal Negative

Your Report : Anti-HBc IgM positive

Anti-HBc IgM equivocal

73

Anti-HBc IgM positive

1

Overall Results

244

-1

% Correct

10

99.6

------------------------------------------------------------UK

2430

Total

1Anti-HBc IgM negative

72

All

98.6

------------------------------------------------------------

2

Score

Your Score : 2

Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG

© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:18 on Thursday 12 July 2018

of 14

Page 6: Hepatitis B serology Laboratory : Distribution : 4202 Page ... · Specimen 4140: 519 IU/mL (16-946) with Abbott Architect; 340 IU/mL (22-1000) with DiaSorin Liaison. Specimen 4142:

Dispatch Date : 01-Jan-2018

Page 6

Hepatitis B serology

Distribution : 4202

Laboratory :

Six specimens were dispatched with the request to report on hepatitis B surface antigen (HBsAg) status and other hepatitis B markers. The specimens were derived from thrombinised human plasma (serum) donations. Specimens 4138, 4139, 4140 and 4142 were diluted 1:9.7, 1:4, 1:242, 1:6.9 respectively in negative thrombinised human plasma (serum). Specimens 4141 and 4143 were pools of negative thrombinised human plasma (serum).

Specimen : 4139 HBsAg positive (0.3 IU/mL) HBeAg negative

UK (%) All (%)

25 (32.5) 29 (10.9)bioMerieux : Vidas31 (40.3) 137 (51.5)Abbott : Architect

0 (0.0) 1 (0.4)Dia.Pro0 (0.0) 2 (0.8)BioRad : Monolisa0 (0.0) 2 (0.8)Unspecified0 (0.0) 4 (1.5)OCD : VITROS1 (1.3) 4 (1.5)DiaSorin3 (3.9) 16 (6.0)DiaSorin : Liaison3 (3.9) 19 (7.1)Siemens:ADVIA Centaur

14 (18.2) 52 (19.5)Roche

Number of Reports0 30 60 90 120 150

Positive Equivocal Negative

Your Report : HBeAg negative

HBeAg equivocal

76

HBeAg positive

258

Overall Results

261

2

% Correct

750

98.9

------------------------------------------------------------UK

30

Total

1HBeAg negative

1

All

98.7

------------------------------------------------------------

-1

Score

Your Score : 2

Specimen : 4139 HBsAg positive (0.3 IU/mL) Anti-HBe positive

UK (%) All (%)

25 (32.5) 27 (10.3)bioMerieux : Vidas31 (40.3) 136 (51.7)Abbott : Architect

0 (0.0) 1 (0.4)Dia.Pro0 (0.0) 1 (0.4)Ortho0 (0.0) 1 (0.4)BioRad : Monolisa0 (0.0) 1 (0.4)Biokit : Bioelisa0 (0.0) 3 (1.1)OCD : VITROS0 (0.0) 3 (1.1)Unspecified1 (1.3) 4 (1.5)DiaSorin3 (3.9) 16 (6.1)DiaSorin : Liaison3 (3.9) 19 (7.2)Siemens:ADVIA Centaur

14 (18.2) 51 (19.4)Roche

Number of Reports0 30 60 90 120 150

Positive Equivocal Negative

Your Report : Anti-HBe positive

Anti-HBe equivocal

76

Anti-HBe positive

54

Overall Results

259

NS

% Correct

1036

65.3

------------------------------------------------------------UK

16918

Total

NSAnti-HBe negative

48

All

63.2

------------------------------------------------------------

NS

Score

Your Score : Not scored

Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG

© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:18 on Thursday 12 July 2018

of 14

Page 7: Hepatitis B serology Laboratory : Distribution : 4202 Page ... · Specimen 4140: 519 IU/mL (16-946) with Abbott Architect; 340 IU/mL (22-1000) with DiaSorin Liaison. Specimen 4142:

Dispatch Date : 01-Jan-2018

Page 7

Hepatitis B serology

Distribution : 4202

Laboratory :

Six specimens were dispatched with the request to report on hepatitis B surface antigen (HBsAg) status and other hepatitis B markers. The specimens were derived from thrombinised human plasma (serum) donations. Specimens 4138, 4139, 4140 and 4142 were diluted 1:9.7, 1:4, 1:242, 1:6.9 respectively in negative thrombinised human plasma (serum). Specimens 4141 and 4143 were pools of negative thrombinised human plasma (serum).

Specimen : 4140 HBsAg positive (22 IU/mL)

UK (%) All (%)

8 (3.5) 9 (1.6)Biokit : Bioelisa60 (26.3) 76 (13.5)bioMerieux : Vidas49 (21.5) 178 (31.7)Architect HBsAg qual II

7 (3.1) 8 (1.4)Beckman : Access3 (1.3) 11 (2.0)Abbott : Architect5 (2.2) 14 (2.5)BioRad : Monolisa7 (3.1) 16 (2.8)Murex2 (0.9) 17 (3.0)Architect HBsAg quant

10 (4.4) 21 (3.7)Neutralisation (all kits)10 (4.4) 27 (4.8)DiaSorin : Liaison14 (6.1) 35 (6.2)Siemens:ADVIA Centaur47 (20.6) 108 (19.2)Roche

Number of Reports0 40 80 120 160 200

Positive Equivocal Negative

Your Report : HBsAg positive

HBsAg equivocal

140

HBsAg positive

4

Overall Results

388

-1

% Correct

00

99.0

------------------------------------------------------------UK

3840

Total

1HBsAg negative

140

All

100

------------------------------------------------------------

2

Score

Your Score : 2

Specimen : 4140 HBsAg positive (22 IU/mL) Anti-HBc positive

UK (%) All (%)

5 (3.2) 6 (1.5)Biokit : Bioelisa47 (30.3) 169 (42.3)Architect anti-HBc II

2 (1.3) 4 (1.0)DiaSorin0 (0.0) 4 (1.0)Abbott : Prism0 (0.0) 5 (1.3)OCD : VITROS6 (3.9) 7 (1.8)Beckman : Access1 (0.6) 8 (2.0)BioRad : Monolisa1 (0.6) 11 (2.8)Abbott : Architect7 (4.5) 18 (4.5)DiaSorin : Liaison9 (5.8) 28 (7.0)Siemens:ADVIA Centaur

35 (22.6) 44 (11.0)bioMerieux : Vidas40 (25.8) 81 (20.3)Roche

Number of Reports0 40 80 120 160 200

Positive Equivocal Negative

Your Report : Anti-HBc positive

Anti-HBc equivocal

130

Anti-HBc positive

0

Overall Results

347

-1

% Correct

00

100

------------------------------------------------------------UK

3470

Total

1Anti-HBc negative

130

All

100

------------------------------------------------------------

2

Score

Your Score : 2

Specimen : 4140 HBsAg positive (22 IU/mL) Anti-HBc IgM negative

UK (%) All (%)

30 (41.7) 121 (50.2)Abbott : Architect1 (1.4) 1 (0.4)BioRad : Monolisa0 (0.0) 1 (0.4)Beckman0 (0.0) 1 (0.4)DiaSorin0 (0.0) 2 (0.8)Dia.Pro1 (1.4) 2 (0.8)Beckman : Access0 (0.0) 2 (0.8)Unspecified0 (0.0) 6 (2.5)OCD : VITROS3 (4.2) 12 (5.0)DiaSorin : Liaison3 (4.2) 18 (7.5)Siemens:ADVIA Centaur

24 (33.3) 28 (11.6)bioMerieux : Vidas10 (13.9) 46 (19.1)Roche

Number of Reports0 30 60 90 120 150

Positive Equivocal Negative

Your Report : Anti-HBc IgM negative

Anti-HBc IgM equivocal

73

Anti-HBc IgM positive

243

Overall Results

245

2

% Correct

730

99.2

------------------------------------------------------------UK

20

Total

1Anti-HBc IgM negative

0

All

100

------------------------------------------------------------

-1

Score

Your Score : 2

Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG

© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:18 on Thursday 12 July 2018

of 14

Page 8: Hepatitis B serology Laboratory : Distribution : 4202 Page ... · Specimen 4140: 519 IU/mL (16-946) with Abbott Architect; 340 IU/mL (22-1000) with DiaSorin Liaison. Specimen 4142:

Dispatch Date : 01-Jan-2018

Page 8

Hepatitis B serology

Distribution : 4202

Laboratory :

Six specimens were dispatched with the request to report on hepatitis B surface antigen (HBsAg) status and other hepatitis B markers. The specimens were derived from thrombinised human plasma (serum) donations. Specimens 4138, 4139, 4140 and 4142 were diluted 1:9.7, 1:4, 1:242, 1:6.9 respectively in negative thrombinised human plasma (serum). Specimens 4141 and 4143 were pools of negative thrombinised human plasma (serum).

Specimen : 4140 HBsAg positive (22 IU/mL) HBeAg negative

UK (%) All (%)

24 (31.6) 28 (10.5)bioMerieux : Vidas31 (40.8) 137 (51.5)Abbott : Architect

0 (0.0) 1 (0.4)GBC EASE0 (0.0) 1 (0.4)Dia.Pro0 (0.0) 2 (0.8)BioRad : Monolisa0 (0.0) 2 (0.8)Unspecified0 (0.0) 4 (1.5)OCD : VITROS1 (1.3) 4 (1.5)DiaSorin3 (3.9) 16 (6.0)DiaSorin : Liaison3 (3.9) 19 (7.1)Siemens:ADVIA Centaur

14 (18.4) 52 (19.5)Roche

Number of Reports0 30 60 90 120 150

Positive Equivocal Negative

Your Report : HBeAg negative

HBeAg equivocal

76

HBeAg positive

261

Overall Results

261

2

% Correct

760

100

------------------------------------------------------------UK

00

Total

1HBeAg negative

0

All

100

------------------------------------------------------------

-1

Score

Your Score : 2

Specimen : 4140 HBsAg positive (22 IU/mL) Anti-HBe positive

UK (%) All (%)

24 (31.6) 27 (10.3)bioMerieux : Vidas31 (40.8) 136 (51.9)Abbott : Architect

0 (0.0) 1 (0.4)Dia.Pro0 (0.0) 1 (0.4)Ortho0 (0.0) 1 (0.4)BioRad : Monolisa0 (0.0) 1 (0.4)Biokit : Bioelisa0 (0.0) 2 (0.8)Unspecified0 (0.0) 3 (1.1)OCD : VITROS1 (1.3) 4 (1.5)DiaSorin3 (3.9) 16 (6.1)DiaSorin : Liaison3 (3.9) 19 (7.3)Siemens:ADVIA Centaur

14 (18.4) 51 (19.5)Roche

Number of Reports0 30 60 90 120 150

Positive Equivocal Negative

Your Report : Anti-HBe positive

Anti-HBe equivocal

76

Anti-HBe positive

20

Overall Results

259

-1

% Correct

15

90.3

------------------------------------------------------------UK

2342

Total

1Anti-HBe negative

73

All

96.1

------------------------------------------------------------

2

Score

Your Score : 2

Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG

© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:18 on Thursday 12 July 2018

of 14

Page 9: Hepatitis B serology Laboratory : Distribution : 4202 Page ... · Specimen 4140: 519 IU/mL (16-946) with Abbott Architect; 340 IU/mL (22-1000) with DiaSorin Liaison. Specimen 4142:

Dispatch Date : 01-Jan-2018

Page 9

Hepatitis B serology

Distribution : 4202

Laboratory :

Six specimens were dispatched with the request to report on hepatitis B surface antigen (HBsAg) status and other hepatitis B markers. The specimens were derived from thrombinised human plasma (serum) donations. Specimens 4138, 4139, 4140 and 4142 were diluted 1:9.7, 1:4, 1:242, 1:6.9 respectively in negative thrombinised human plasma (serum). Specimens 4141 and 4143 were pools of negative thrombinised human plasma (serum).

Specimen : 4141 HBsAg negative

UK (%) All (%)

8 (4.2) 9 (1.8)Biokit : Bioelisa40 (21.1) 54 (11.0)bioMerieux : Vidas48 (25.3) 173 (35.2)Architect HBsAg qual II

0 (0.0) 7 (1.4)OCD : VITROS7 (3.7) 8 (1.6)Beckman : Access2 (1.1) 9 (1.8)Abbott : Architect5 (2.6) 12 (2.4)Murex2 (1.1) 13 (2.6)Architect HBsAg quant5 (2.6) 14 (2.8)BioRad : Monolisa9 (4.7) 23 (4.7)DiaSorin : Liaison

13 (6.8) 34 (6.9)Siemens:ADVIA Centaur45 (23.7) 104 (21.1)Roche

Number of Reports0 40 80 120 160 200

Positive Equivocal Negative

Your Report : HBsAg negative

HBsAg equivocal

140

HBsAg positive

387

Overall Results

388

2

% Correct

1400

99.7

------------------------------------------------------------UK

10

Total

1HBsAg negative

0

All

100

------------------------------------------------------------

-1

Score

Your Score : 2

Specimen : 4141 HBsAg negative Anti-HBc negative

UK (%) All (%)

5 (3.7) 6 (1.6)Biokit : Bioelisa40 (29.6) 158 (42.2)Architect anti-HBc II

0 (0.0) 3 (0.8)Unspecified0 (0.0) 4 (1.1)Abbott : Prism0 (0.0) 5 (1.3)OCD : VITROS6 (4.4) 7 (1.9)Beckman : Access1 (0.7) 8 (2.1)BioRad : Monolisa1 (0.7) 9 (2.4)Abbott : Architect6 (4.4) 18 (4.8)DiaSorin : Liaison8 (5.9) 26 (7.0)Siemens:ADVIA Centaur

30 (22.2) 39 (10.4)bioMerieux : Vidas36 (26.7) 77 (20.6)Roche

Number of Reports0 40 80 120 160 200

Positive Equivocal Negative

Your Report : Anti-HBc negative

Anti-HBc equivocal

114

Anti-HBc positive

327

Overall Results

327

2

% Correct

1140

100

------------------------------------------------------------UK

00

Total

1Anti-HBc negative

0

All

100

------------------------------------------------------------

-1

Score

Your Score : 2

Specimen : 4141 HBsAg negative Anti-HBc IgM negative

UK (%) All (%)

16 (40.0) 94 (49.2)Abbott : Architect0 (0.0) 1 (0.5)Dia.Pro0 (0.0) 1 (0.5)Beckman0 (0.0) 1 (0.5)DiaSorin1 (2.5) 2 (1.0)Beckman : Access0 (0.0) 2 (1.0)Unspecified0 (0.0) 5 (2.6)OCD : VITROS1 (2.5) 11 (5.8)DiaSorin : Liaison2 (5.0) 15 (7.9)Siemens:ADVIA Centaur

14 (35.0) 17 (8.9)bioMerieux : Vidas6 (15.0) 42 (22.0)Roche

Number of Reports0 20 40 60 80 100

Positive Equivocal Negative

Your Report : Anti-HBc IgM negative

Anti-HBc IgM equivocal

41

Anti-HBc IgM positive

194

Overall Results

194

2

% Correct

410

100

------------------------------------------------------------UK

00

Total

1Anti-HBc IgM negative

0

All

100

------------------------------------------------------------

-1

Score

Your Score : 2

Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG

© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:18 on Thursday 12 July 2018

of 14

Page 10: Hepatitis B serology Laboratory : Distribution : 4202 Page ... · Specimen 4140: 519 IU/mL (16-946) with Abbott Architect; 340 IU/mL (22-1000) with DiaSorin Liaison. Specimen 4142:

Dispatch Date : 01-Jan-2018

Page 10

Hepatitis B serology

Distribution : 4202

Laboratory :

Six specimens were dispatched with the request to report on hepatitis B surface antigen (HBsAg) status and other hepatitis B markers. The specimens were derived from thrombinised human plasma (serum) donations. Specimens 4138, 4139, 4140 and 4142 were diluted 1:9.7, 1:4, 1:242, 1:6.9 respectively in negative thrombinised human plasma (serum). Specimens 4141 and 4143 were pools of negative thrombinised human plasma (serum).

Specimen : 4141 HBsAg negative HBeAg negative

UK (%) All (%)

14 (35.0) 16 (7.7)bioMerieux : Vidas14 (35.0) 104 (50.0)Abbott : Architect

0 (0.0) 1 (0.5)GBC EASE0 (0.0) 2 (1.0)BioRad : Monolisa0 (0.0) 2 (1.0)Unspecified0 (0.0) 3 (1.4)OCD : VITROS0 (0.0) 3 (1.4)DiaSorin2 (5.0) 15 (7.2)DiaSorin : Liaison2 (5.0) 16 (7.7)Siemens:ADVIA Centaur8 (20.0) 46 (22.1)Roche

Number of Reports0 30 60 90 120 150

Positive Equivocal Negative

Your Report : HBeAg negative

HBeAg equivocal

41

HBeAg positive

206

Overall Results

206

2

% Correct

410

100

------------------------------------------------------------UK

00

Total

1HBeAg negative

0

All

100

------------------------------------------------------------

-1

Score

Your Score : 2

Specimen : 4141 HBsAg negative Anti-HBe negative

UK (%) All (%)

13 (33.3) 14 (6.8)bioMerieux : Vidas14 (35.9) 105 (51.2)Abbott : Architect

0 (0.0) 1 (0.5)BioRad : Monolisa0 (0.0) 1 (0.5)Biokit : Bioelisa0 (0.0) 2 (1.0)Unspecified0 (0.0) 3 (1.5)OCD : VITROS0 (0.0) 3 (1.5)DiaSorin2 (5.1) 15 (7.3)DiaSorin : Liaison2 (5.1) 16 (7.8)Siemens:ADVIA Centaur8 (20.5) 45 (22.0)Roche

Number of Reports0 30 60 90 120 150

Positive Equivocal Negative

Your Report : Anti-HBe negative

Anti-HBe equivocal

40

Anti-HBe positive

204

Overall Results

204

2

% Correct

400

100

------------------------------------------------------------UK

00

Total

1Anti-HBe negative

0

All

100

------------------------------------------------------------

-1

Score

Your Score : 2

Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG

© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:18 on Thursday 12 July 2018

of 14

Page 11: Hepatitis B serology Laboratory : Distribution : 4202 Page ... · Specimen 4140: 519 IU/mL (16-946) with Abbott Architect; 340 IU/mL (22-1000) with DiaSorin Liaison. Specimen 4142:

Dispatch Date : 01-Jan-2018

Page 11

Hepatitis B serology

Distribution : 4202

Laboratory :

Six specimens were dispatched with the request to report on hepatitis B surface antigen (HBsAg) status and other hepatitis B markers. The specimens were derived from thrombinised human plasma (serum) donations. Specimens 4138, 4139, 4140 and 4142 were diluted 1:9.7, 1:4, 1:242, 1:6.9 respectively in negative thrombinised human plasma (serum). Specimens 4141 and 4143 were pools of negative thrombinised human plasma (serum).

Specimen : 4142 HBsAg positive (1798 IU/mL)

UK (%) All (%)

8 (3.5) 9 (1.6)Biokit : Bioelisa60 (26.5) 76 (13.6)bioMerieux : Vidas49 (21.7) 177 (31.7)Architect HBsAg qual II

7 (3.1) 8 (1.4)Beckman : Access3 (1.3) 11 (2.0)Abbott : Architect5 (2.2) 14 (2.5)BioRad : Monolisa6 (2.7) 15 (2.7)Murex2 (0.9) 17 (3.0)Architect HBsAg quant

10 (4.4) 21 (3.8)Neutralisation (all kits)9 (4.0) 26 (4.7)DiaSorin : Liaison

14 (6.2) 35 (6.3)Siemens:ADVIA Centaur47 (20.8) 108 (19.3)Roche

Number of Reports0 40 80 120 160 200

Positive Equivocal Negative

Your Report : HBsAg positive

HBsAg equivocal

140

HBsAg positive

0

Overall Results

387

-1

% Correct

00

100

------------------------------------------------------------UK

3870

Total

1HBsAg negative

140

All

100

------------------------------------------------------------

2

Score

Your Score : 2

Specimen : 4142 HBsAg positive (1798 IU/mL) Anti-HBc positive

UK (%) All (%)

5 (3.2) 6 (1.5)Biokit : Bioelisa47 (30.5) 169 (42.4)Architect anti-HBc II

2 (1.3) 4 (1.0)DiaSorin0 (0.0) 4 (1.0)Abbott : Prism0 (0.0) 5 (1.3)OCD : VITROS6 (3.9) 7 (1.8)Beckman : Access1 (0.6) 8 (2.0)BioRad : Monolisa1 (0.6) 11 (2.8)Abbott : Architect7 (4.5) 18 (4.5)DiaSorin : Liaison9 (5.8) 28 (7.0)Siemens:ADVIA Centaur

34 (22.1) 43 (10.8)bioMerieux : Vidas40 (26.0) 81 (20.3)Roche

Number of Reports0 40 80 120 160 200

Positive Equivocal Negative

Your Report : Anti-HBc positive

Anti-HBc equivocal

130

Anti-HBc positive

1

Overall Results

347

-1

% Correct

00

99.7

------------------------------------------------------------UK

3460

Total

1Anti-HBc negative

130

All

100

------------------------------------------------------------

2

Score

Your Score : 2

Specimen : 4142 HBsAg positive (1798 IU/mL) Anti-HBc IgM negative

UK (%) All (%)

30 (41.7) 120 (49.8)Abbott : Architect1 (1.4) 1 (0.4)BioRad : Monolisa0 (0.0) 1 (0.4)Beckman0 (0.0) 1 (0.4)DiaSorin0 (0.0) 2 (0.8)Dia.Pro1 (1.4) 2 (0.8)Beckman : Access0 (0.0) 2 (0.8)Unspecified0 (0.0) 6 (2.5)OCD : VITROS3 (4.2) 13 (5.4)DiaSorin : Liaison3 (4.2) 18 (7.5)Siemens:ADVIA Centaur

24 (33.3) 28 (11.6)bioMerieux : Vidas10 (13.9) 46 (19.1)Roche

Number of Reports0 30 60 90 120 150

Positive Equivocal Negative

Your Report : Anti-HBc IgM negative

Anti-HBc IgM equivocal

73

Anti-HBc IgM positive

243

Overall Results

245

2

% Correct

730

99.2

------------------------------------------------------------UK

20

Total

1Anti-HBc IgM negative

0

All

100

------------------------------------------------------------

-1

Score

Your Score : 2

Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG

© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:18 on Thursday 12 July 2018

of 14

Page 12: Hepatitis B serology Laboratory : Distribution : 4202 Page ... · Specimen 4140: 519 IU/mL (16-946) with Abbott Architect; 340 IU/mL (22-1000) with DiaSorin Liaison. Specimen 4142:

Dispatch Date : 01-Jan-2018

Page 12

Hepatitis B serology

Distribution : 4202

Laboratory :

Six specimens were dispatched with the request to report on hepatitis B surface antigen (HBsAg) status and other hepatitis B markers. The specimens were derived from thrombinised human plasma (serum) donations. Specimens 4138, 4139, 4140 and 4142 were diluted 1:9.7, 1:4, 1:242, 1:6.9 respectively in negative thrombinised human plasma (serum). Specimens 4141 and 4143 were pools of negative thrombinised human plasma (serum).

Specimen : 4142 HBsAg positive (1798 IU/mL) HBeAg negative

UK (%) All (%)

24 (31.6) 28 (10.6)bioMerieux : Vidas31 (40.8) 136 (51.3)Abbott : Architect

0 (0.0) 1 (0.4)GBC EASE0 (0.0) 1 (0.4)Dia.Pro0 (0.0) 2 (0.8)BioRad : Monolisa0 (0.0) 2 (0.8)Unspecified0 (0.0) 4 (1.5)OCD : VITROS1 (1.3) 4 (1.5)DiaSorin3 (3.9) 16 (6.0)DiaSorin : Liaison3 (3.9) 19 (7.2)Siemens:ADVIA Centaur

14 (18.4) 52 (19.6)Roche

Number of Reports0 30 60 90 120 150

Positive Equivocal Negative

Your Report : HBeAg negative

HBeAg equivocal

76

HBeAg positive

260

Overall Results

260

2

% Correct

760

100

------------------------------------------------------------UK

00

Total

1HBeAg negative

0

All

100

------------------------------------------------------------

-1

Score

Your Score : 2

Specimen : 4142 HBsAg positive (1798 IU/mL) Anti-HBe positive

UK (%) All (%)

24 (31.6) 26 (10.0)bioMerieux : Vidas31 (40.8) 136 (52.1)Abbott : Architect

0 (0.0) 1 (0.4)Dia.Pro0 (0.0) 1 (0.4)Ortho0 (0.0) 1 (0.4)BioRad : Monolisa0 (0.0) 1 (0.4)Biokit : Bioelisa0 (0.0) 2 (0.8)Unspecified0 (0.0) 3 (1.1)OCD : VITROS1 (1.3) 4 (1.5)DiaSorin3 (3.9) 16 (6.1)DiaSorin : Liaison3 (3.9) 19 (7.3)Siemens:ADVIA Centaur

14 (18.4) 51 (19.5)Roche

Number of Reports0 30 60 90 120 150

Positive Equivocal Negative

Your Report : Anti-HBe positive

Anti-HBe equivocal

76

Anti-HBe positive

0

Overall Results

259

-1

% Correct

00

100

------------------------------------------------------------UK

2590

Total

1Anti-HBe negative

76

All

100

------------------------------------------------------------

2

Score

Your Score : 2

Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG

© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:18 on Thursday 12 July 2018

of 14

Page 13: Hepatitis B serology Laboratory : Distribution : 4202 Page ... · Specimen 4140: 519 IU/mL (16-946) with Abbott Architect; 340 IU/mL (22-1000) with DiaSorin Liaison. Specimen 4142:

Dispatch Date : 01-Jan-2018

Page 13

Hepatitis B serology

Distribution : 4202

Laboratory :

Six specimens were dispatched with the request to report on hepatitis B surface antigen (HBsAg) status and other hepatitis B markers. The specimens were derived from thrombinised human plasma (serum) donations. Specimens 4138, 4139, 4140 and 4142 were diluted 1:9.7, 1:4, 1:242, 1:6.9 respectively in negative thrombinised human plasma (serum). Specimens 4141 and 4143 were pools of negative thrombinised human plasma (serum).

Specimen : 4143 HBsAg negative

UK (%) All (%)

7 (3.7) 8 (1.6)Biokit : Bioelisa39 (20.7) 53 (10.8)bioMerieux : Vidas48 (25.5) 172 (35.2)Architect HBsAg qual II

0 (0.0) 7 (1.4)OCD : VITROS7 (3.7) 8 (1.6)Beckman : Access2 (1.1) 9 (1.8)Abbott : Architect5 (2.7) 12 (2.5)Murex2 (1.1) 13 (2.7)Architect HBsAg quant5 (2.7) 14 (2.9)BioRad : Monolisa9 (4.8) 23 (4.7)DiaSorin : Liaison

13 (6.9) 34 (7.0)Siemens:ADVIA Centaur45 (23.9) 104 (21.3)Roche

Number of Reports0 40 80 120 160 200

Positive Equivocal Negative

Your Report : HBsAg negative

HBsAg equivocal

139

HBsAg positive

385

Overall Results

386

2

% Correct

1390

99.7

------------------------------------------------------------UK

10

Total

1HBsAg negative

0

All

100

------------------------------------------------------------

-1

Score

Your Score : 2

Specimen : 4143 HBsAg negative Anti-HBc negative

UK (%) All (%)

5 (3.7) 6 (1.6)Biokit : Bioelisa40 (29.4) 158 (42.1)Architect anti-HBc II

0 (0.0) 3 (0.8)Unspecified0 (0.0) 4 (1.1)Abbott : Prism0 (0.0) 5 (1.3)OCD : VITROS6 (4.4) 7 (1.9)Beckman : Access1 (0.7) 8 (2.1)BioRad : Monolisa1 (0.7) 10 (2.7)Abbott : Architect6 (4.4) 18 (4.8)DiaSorin : Liaison8 (5.9) 26 (6.9)Siemens:ADVIA Centaur

31 (22.8) 40 (10.7)bioMerieux : Vidas36 (26.5) 76 (20.3)Roche

Number of Reports0 40 80 120 160 200

Positive Equivocal Negative

Your Report : Anti-HBc negative

Anti-HBc equivocal

114

Anti-HBc positive

325

Overall Results

326

2

% Correct

1140

99.7

------------------------------------------------------------UK

10

Total

1Anti-HBc negative

0

All

100

------------------------------------------------------------

-1

Score

Your Score : 2

Specimen : 4143 HBsAg negative Anti-HBc IgM negative

UK (%) All (%)

16 (40.0) 91 (48.4)Abbott : Architect0 (0.0) 1 (0.5)Dia.Pro0 (0.0) 1 (0.5)Beckman0 (0.0) 1 (0.5)DiaSorin1 (2.5) 2 (1.1)Beckman : Access0 (0.0) 2 (1.1)Unspecified0 (0.0) 5 (2.7)OCD : VITROS1 (2.5) 11 (5.9)DiaSorin : Liaison2 (5.0) 15 (8.0)Siemens:ADVIA Centaur

14 (35.0) 17 (9.0)bioMerieux : Vidas6 (15.0) 42 (22.3)Roche

Number of Reports0 20 40 60 80 100

Positive Equivocal Negative

Your Report : Anti-HBc IgM negative

Anti-HBc IgM equivocal

41

Anti-HBc IgM positive

191

Overall Results

191

2

% Correct

410

100

------------------------------------------------------------UK

00

Total

1Anti-HBc IgM negative

0

All

100

------------------------------------------------------------

-1

Score

Your Score : 2

Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG

© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:18 on Thursday 12 July 2018

of 14

Page 14: Hepatitis B serology Laboratory : Distribution : 4202 Page ... · Specimen 4140: 519 IU/mL (16-946) with Abbott Architect; 340 IU/mL (22-1000) with DiaSorin Liaison. Specimen 4142:

Dispatch Date : 01-Jan-2018

Page 14

Hepatitis B serology

Distribution : 4202

Laboratory :

Six specimens were dispatched with the request to report on hepatitis B surface antigen (HBsAg) status and other hepatitis B markers. The specimens were derived from thrombinised human plasma (serum) donations. Specimens 4138, 4139, 4140 and 4142 were diluted 1:9.7, 1:4, 1:242, 1:6.9 respectively in negative thrombinised human plasma (serum). Specimens 4141 and 4143 were pools of negative thrombinised human plasma (serum).

Specimen : 4143 HBsAg negative HBeAg negative

UK (%) All (%)

14 (35.0) 16 (7.7)bioMerieux : Vidas14 (35.0) 104 (50.0)Abbott : Architect

0 (0.0) 1 (0.5)GBC EASE0 (0.0) 2 (1.0)BioRad : Monolisa0 (0.0) 2 (1.0)Unspecified0 (0.0) 3 (1.4)OCD : VITROS0 (0.0) 3 (1.4)DiaSorin2 (5.0) 15 (7.2)DiaSorin : Liaison2 (5.0) 16 (7.7)Siemens:ADVIA Centaur8 (20.0) 46 (22.1)Roche

Number of Reports0 30 60 90 120 150

Positive Equivocal Negative

Your Report : HBeAg negative

HBeAg equivocal

41

HBeAg positive

205

Overall Results

206

2

% Correct

410

99.5

------------------------------------------------------------UK

10

Total

1HBeAg negative

0

All

100

------------------------------------------------------------

-1

Score

Your Score : 2

Specimen : 4143 HBsAg negative Anti-HBe negative

UK (%) All (%)

14 (35.0) 15 (7.3)bioMerieux : Vidas14 (35.0) 105 (51.0)Abbott : Architect

0 (0.0) 1 (0.5)BioRad : Monolisa0 (0.0) 1 (0.5)Biokit : Bioelisa0 (0.0) 2 (1.0)Unspecified0 (0.0) 3 (1.5)OCD : VITROS0 (0.0) 3 (1.5)DiaSorin2 (5.0) 15 (7.3)DiaSorin : Liaison2 (5.0) 16 (7.8)Siemens:ADVIA Centaur8 (20.0) 45 (21.8)Roche

Number of Reports0 30 60 90 120 150

Positive Equivocal Negative

Your Report : Anti-HBe negative

Anti-HBe equivocal

41

Anti-HBe positive

205

Overall Results

205

2

% Correct

410

100

------------------------------------------------------------UK

00

Total

1Anti-HBe negative

0

All

100

------------------------------------------------------------

-1

Score

Your Score : 2

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© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:18 on Thursday 12 July 2018

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