heat stress (hs) in blueberry final report 1 1 april, 2013
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Heat Stress (HS) in Blueberry Final report 1 1 April, 2013. Temperature Sensor. c yclic nucleotide-gated calcium channel Arabidopsis has two paralogs (CNG2 and CNG4) . - PowerPoint PPT PresentationTRANSCRIPT
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Heat Stress (HS) in BlueberryFinal report 11 April, 2013
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• cyclic nucleotide-gated calcium channel
• Arabidopsis has two paralogs (CNG2 and CNG4)
Temperature Sensor
Andrija Finka, America Farinia Henriquez Cuendet, Frans J.M. Maathuis, Younousse Saidi, and Pierre Goloubinoffa. . Plasma Membrane Cyclic Nucleotide Gated Calcium Channels Control Land Plant Thermal Sensing and Acquired Thermotolerance. The Plant Cell, Vol. 24: 3333–3348, August 2012.
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Conserved Family of Genes
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Deletions Grow Slower 20° C
Andrija Finka, America Farinia Henriquez Cuendet, Frans J.M. Maathuis, Younousse Saidi, and Pierre Goloubinoffa. . Plasma Membrane Cyclic Nucleotide Gated Calcium Channels Control Land Plant Thermal Sensing and Acquired Thermotolerance. The Plant Cell, Vol. 24: 3333–3348, August 2012.
Arabidopsis Moss
WT
CNGC2-/-
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CNGC2-/- Hyper-Thermosensitive
Moss
HSP
prom
oter
+ R
epor
ter
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More Calcium Enters CNGC2-/- Moss
WTCNGC2-/-
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3 Temp-Sensitive Ca2+ ChannelsMoss
conductance inpicosiemens
(1 – 100 range)
WT CNGC2-/-
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Prime Plants for ThermotoleranceArabidopsis
WT
CNGC2-/-
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Full HSR at Lower TempsArabidopsis
WT CNGC2-/-
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Survive Extreme Heat TreatmentWTCNGC2-/-
Arabidopsis
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Post Temperature Sensor
1. calcium channel in PM (primary pathway)2. histone sensor in the nucleus3. unfolded protein sensor (UPR) in the endoplasmic reticulum4. unfolded protein sensor (UPR) in the cytosol
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• tolerant NCED4 allele is responsible for germination at
high temperatures in lettuce
• encodes 9-cis-epoxycarotenoid dioxygenase 4
(1.13.11.51)
• Catalyzes the first step of abscisic-acid biosynthesis
from carotenoids in chloroplasts, in response to water
stress
Seed Germination
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NCED4 Determines Heat Inhibition
wt NCED4
inhib NCED4
inhib NCED4 + RNAi
tolerant NCED4
heat tolerant seeds
heat inhibited seeds
Heqiang Huo, Peetambar Dahal, Keshavulu Kunusoth, Claire M. McCallum, and Kent J. Bradford.2013 ePublication. Expression of 9-cis-EPOXYCAROTENOID DIOXYGENASE4 Is Essential for Thermoinhibition of Lettuce Seed Germinationbut Not for Seed Development or Stress Tolerance. www.plantcell.org/cgi/doi/10.1105/tpc.112.108902.
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NCED4 Determines Heat Inhibition
wt NCED4
inhib NCED4
inhib NCED4 + RNAi
tolerant NCED4
heat tolerant seeds
heat inhibited seeds
Heqiang Huo, Peetambar Dahal, Keshavulu Kunusoth, Claire M. McCallum, and Kent J. Bradford.2013 ePublication. Expression of 9-cis-EPOXYCAROTENOID DIOXYGENASE4 Is Essential for Thermoinhibition of Lettuce Seed Germinationbut Not for Seed Development or Stress Tolerance. www.plantcell.org/cgi/doi/10.1105/tpc.112.108902.
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HS Pathway
• reverts when cooled and HsfB antagonism wins
• HS response requires heterotrimer of HsfA+B+C• HsfA1 (family) is the master regulator
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HsfA1-independent Pathwayheat may be able to activate HS response without HsfA1
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genes involved in thermotolerance
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List of Genes with 51 SSRs Primer Pairs xnWRKY 3 scaffold00003 x 3RBOHD & E scaffold00029 x 3BIP1 scaffold00039 x3MBF1 scaffold00048 x3H2A.Z aka HTA11 scaffold00069 x4CNGC2 & CNGC4 scaffold00079 x3CAT1 scaffold00093 x3HSFA2 scaffold00109 x3ETR1 scaffold00112 x3FAD6 scaffold00117 x3HSFB1 scaffold00122 x3FAD3 scaffold00126 x3PLD scaffold00152 x3ABI3 scaffold00173 x3UVH6 scaffold00209 x3CAD2 scaffold00210 x3VTC2 scaffold00252 x3NPQ1 scaffold00260 x3ABI1 scaffold00338 x3RBOHA & C & G & H scaffold02317 x2
FAD5 scaffold00455 x3ACD2 scaffold00474 x3RBOHB scaffold00539 x3FAD4 scaffold00588 x3BZIP28 scaffold00604 x3BZIP17 scaffold00617 x3IRE1 scaffold00646 x3DREB scaffold00667 x3MS1 scaffold00725 x3BIP2 scaffold00773 x3NCED4 scaffold00790 x2VTC1 scaffold00867 x3CAM3 scaffold00875 x3CDPK 2 scaffold00916 x3ETO1 scaffold00956 x3AXR1 scaffold01037 x3PIPK scaffold01039 x3HSFA1 scaffold01062 x3RBOHF scaffold01076 x1
UBP2 scaffold01164 x3ABI2 scaffold01374 x3HOT1 scaffold01438 x3ETO2 & 3 scaffold01451 x3UVH3 scaffold01548 x2UVH1 scaffold02430 x2ETO1 #2 scaffold01626 x2FAD2 scaffold01825 x2EIN2 scaffold02031 CDUBP1 scaffold02114 x2FAD7 scaffold02449 x1UTR3 scaffold02795 x1BZIP60 no scaffoldNPR1 scaffold12427 x0
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ReferencesAndrija Finka et al. 2012. Plasma Membrane Cyclic Nucleotide Gated Calcium Channels Control Land Plant Thermal Sensing and Acquired Thermotolerance. The Plant Cell. Vol. 24: 3333–3348.
Heqiang Huo et al.2013 ePublication. Expression of 9-cis-EPOXYCAROTENOID DIOXYGENASE4 Is Essential for Thermoinhibition of Lettuce Seed Germination but Not for Seed Development or Stress Tolerance. www.plantcell.org/cgi/doi/10.1105/tpc.112.108902.
Jane Larkindale et al. 2005. Heat Stress Phenotypes of Arabidopsis Mutants Implicate Multiple Signaling Pathways in the Acquisition of Thermotolerance. Plant Physiology. Vol. 138: 882–897.
Sachin Kotak et al. 2007. A Novel Transcriptional Cascade Regulating Expression of Heat Stress Proteins duringSeedDevelopmentof Arabidopsis. The Plant Cell, Vol. 19: 182–195.
Ching-Hui Yeha et al. 2012. Some like it hot, some like it warm: Phenotyping to explore thermotolerance diversity. Plant Science. Vol. 195: 10-23.
Yong-Xiang Lin et al. 2011. Genome-wide identification, classification and analysis of heat shock transcription factor family in maize. BMC Genomics. Vol. 12: 76.
Hsiang-chin Liu and Yee-yung Charng. 2012. Acquired thermotolerance independent of heat shock factor A1 (HsfA1),the master regulator of the heat stress response. Plant Signaling & Behavior 7(5): 547–550.
Jason Argyris, et al. 2011. A gene encoding an abscisic acid biosynthetic enzyme (LsNCED4) collocates with the high temperature germination locus Htg6.1 in lettuce (Lactuca sp.) Theor Appl Genet. Vol. 122:95–108.
Younousse Saidi, et al. 2009. The Heat Shock Response in Moss Plants Is Regulated by Specific Calcium-Permeable Channels in the Plasma Membrane. The Plant Cell. Vol. 21: 2829–2843.
Ondrej Krinke, et al. 2009. Phospholipase D Activation Is an Early Component of the Salicylic Acid Signaling Pathway in Arabidopsis Cell Suspensions. Plant Physiology. Vol. 150, pp. 424–436.