35th International Society for Heart Research

North American Section Meeting

“HEART FAILURE: 21st CENTURY RESEARCH and THERAPEUTICS”

June 7 -10, 2015

Seattle, Washington

Program Co - Chairs: Charles E. Murry, Rong Tian and Michael Regnier

International Society for Heart Research - North American Section

President: Elizabeth Murphy President-Elect: Gary Lopaschuk Past -President: Donald M. Bers Secretary: Jennifer Van Eyk Treasurer: Litsa Kranias Executive Secretary: Leslie Anderson Lobaugh

American section web site: www.american.ishrworld.org International web site: www.ishrworld.org

Council Members 2009 -15 Mark Anderson Susan Howlett Charles Murry Jeff Robbins Howard Rockman Mark Sussman Jil Tardiff Yibin Wang Chen Gao ECI representative (2013-2015) Stephen Lange ECI representative (2014-2016) Council Member 2015-21 Dale Abel Burns Blaxall John Elrod Joseph Hill Tim OConnell Brian ORourke Monte Willis

Council Members 2012 -18 Chris Baines Pieter de Tombe Asa Gustaffson Joan Heller Brown Timothy Kamp Walter Koch Gary Lopaschuk Jun Sadoshima

Acknowledgements

Thank you to our Sponsors

PLATINUM

Durward & Susan Huckabay Foundation SeaBear

University of Washington, School of Medicine

GOLD

Amgen Gilead Sciences, Inc.

Merck UCLA Proteomics Center

University of Washington, Division of Cardiology University of Washington, Institute for Stem Cell & Regenerative Medicine

University of Washington, Center for Cardiovascular Biology University of Washington, Mitochondria & Metabolism Center

Anonymous

IRIDIUM

Temple University, Cardiovascular Research Center & Center for Translational Medicine VisualSonics

Pfizer IonOptics

PeproTech University of Washington, Deparatment of Bioengineering

SILVER

Research and Diagnostic Systems, Inc.

Special Thanks to Our Meeting Organizers:

Conference Planner: Martha Lee Executive Secretary: Leslie Anderson Lobaugh

Sunday June 7, 2015

Overview

8:00 AM 6:00 PM Speaker Ready Room Favorita

8:00 AM 5:00 PM Registration Leonesa Foyer

8:30 AM 10:30 AM ECI Symposium Eliza Anderson Amphitheater

10:30 AM 10:45 AM Coffee Break Leonesa Foyer

10:45 AM 11:45 AM Career Devleopment Panel - Discussion Eliza Anderson Amphitheater

12:00 PM 1:30 PM ECI Lunch with Invited Senior Investigators (Ticket required)

Il Fornaio 600 Pine St, Suite 132 Seattle, WA 98101

2:00 PM 3:30 PM Young Investigator Competition-Junior Sci. Eliza Anderson Amphitheater

3:30 PM 4:00 PM Coffee Break Leonesa Foyer

4:00 PM 5:30 PM Young Investigator Competition-Senior Sci. Leonesa I/II

6:00 PM 7:00 PM Opening Keynote Speaker – Chris Murray Leonesa I/II

7:15 PM 9:00 PM Welcome Reception & Posters -Session 1

(001-078) Princessa Ballroom and Foyer

8:30 – 10:30 ECI Symposium Chairs: Chen Gao, UCLA and Samarjit Das, Johns Hopkins University 8:30 am Megan Montgomery, UCSF, An alpha-1A adrenergic receptor agonist to treat heart failure

8:42 am Sara Menazza, NHLBI, Non-nuclear estrogen receptor activation reduces cardiac ischemic-reperfusion injury in mice with cardiac specific ablation of ER-alpha

8:54 am Patrick McLendon, Cincinnati Childrens Hospital Med. Ctr., A High-Content RNAi Screen for Novel Effectors of Cardiac Proteotoxicity 9:06 am Vanessa Lina, University of San Paulo, The E487K variant of aldehyde dehydrogenase 2 protects cardiac mitochondrial metabolism in heart failure in mice.

9:18 am Catherine Passariello, University of Miami, Type 3 p90 ribosomal S6 kinase is required for concentric myocyte hypertrophy in a mouse model for Noonan syndrome

9:30 am Jan Schilling, San Diego VA, Novel roles for Chromogranin A peptide catestatin in cardiac metabolism and physiology 9:42 am Randi Parks, NHLBI, Characterization of mitochondria from mice lacking the mitochondrial calcium uniporter 9:54 am Nathan Roe, University of Washington, Deletion of Diacylglycerol:acyltransferase 1 (DGAT1) selectively reduces incorporation of 16:0 fatty acid into triglyceride and alters cardiac metabolism 10:06 am Christopher Murray, University of British Columbia, Determining IKS β-Subunit Stoichiometry Using Unnatural Amino Acid Mutagenesis 10:18 am Wei Guo, University of Wyoming, S107 improves RBM20 deficiency-induced cardiac dysfunction 10:30 – 10:45 Coffee Break 10:45 -11:45 AM Career Development Panel - Alternative Scientific Careers Panel Discussion (Part II) Chair: Mark Kohr, Johns Hopkins University Sakthivel Sadayappan, Loyola University Chicago Panelists: Drew Carlson, Program Officer, National Heart Lung and Blood Institute Steven Houser, Chair and Professor, Temple University Jeffery Molkentin, Professor, Cincinnati Children’s Research Institute Howard Rockman, Professor, Duke University

2:00 – 3:30 PM Young Investigator Competition (Junior) Chair: Jun Sadoshima 2:00-2:15 Geoffrey De Couto-Cedar Sinai, Distinctive macrophage polarization mediates the beneficial effects of

cell therapy in acute myocardial infarction 2:20-2:35 Danny El-Nachef-University of Washington and UCLA, H3K9me3 is required for adult cardiac myocyte

cell cycle exit and gene silencing in vivo. 2:40-2:55 Emma Monte-UCLA, Reciprocal regulation of cardiac chromatin by HMGB and CTCF: implications for

transcriptional regulation in pathologic hypertrophy 3:00-3:15 Pearl Qiujada-SDSU, Enhancement of myocardial repair with cardiac progenitor cell and mesenchymal

stem cell hybrids 4:00 – 5:30 PM Young Investigator Competition (Senior) Chair: Gary Lopaschuk 4:00-4:15 Sam Das-Johns Hopkins, Role of miR-181 family in heart failure: a tale of two intracellular cardiomyocyte

compartments

4:20-4:35 Mohsin Khan-Temple University, Embryonic stem cell-derived exosomes promote endogenous repair

mechanisms and enhance cardiac function following myocardial infarction

4:40-4:55 Nathan Palpant-University of Washington, Human cardiac, endothelial and blood lineages are controlled

by gradients of activing A, BMP4, and Wnt/β-catenin signaling

5:00-5:15 Zhihua Wang-UCLA, Chaer lncRNA negatively regulates PRC2 during cardiac hypertrophy

6:00 - 7:00 PM – Opening Keynote Speaker Chair: Charles M. Murry, MD, PhD

Christopher Murray, MD, PhD Institute Director, Institute for Health Metrics and Evaluation

Professor of Global Health University of Washington

Talk Title: The Global Burden of Ischemic Heart Disease

7:15 - 9:00 PM Welcome Reception & Poster Session I (posters 1-78)

Monday June 8, 2015

Overview

8:00AM 5:00PM Registration Leonesa Foyer

8:00 AM 6:00 PM Speaker Ready Room Favorita

8:00 AM 8:45 AM Plenary Talk – Research Achievement Award Leonesa I/II

9:00 AM 10:30 AM Interest Group 1 Leonesa I/II

9:00 AM 10:30 AM Interest Group 5 Eliza Anderson Amphitheater

10:30 AM 11:00 AM Coffee Break Leonesa Foyer

11:00 AM 12:30 AM Interest Group 3 Leonesa I/II

11:00 AM 12:30 PM Interest Group 4 Eliza Anderson Amphitheater

12:30 PM 1:30 PM ISHR – NAS Council Meeting Menzies Suite – 6th floor

12:30 PM 2:00 PM Lunch & Posters (79-156) Leonesa Foyer

2:00 PM 3:30 PM Interest Group 2 Eliza Anderson Amphitheater

2:00 PM 3:30 PM Session I Leonesa I/II

3:30 PM 4:00 PM Coffee Break Leonesa Foyer

4:00 PM 5:30 PM Session II Leonessa I/II

4:00 PM 5:30 PM Session III Eliza Anderson Amphitheater

8:00 AM – 9:00 AM Plenary Talk – Research Achievement Award Chairs: Rick Moss and Litsa Kranias

Mark Anderson, MD, PhD Director of the Department of Medicine, Johns Hopkins University

Physician in Chief, The Johns Hopkins Hospital Talk title: “Is CaMKII essential for coupling oxidative stress to cardiopulmonary disease?"

9:00 AM – 10:30 AM – Interest Group 1 Session – Heart Failure and Signaling Chairs: Federica del Monte, Harvard University and Stephen Lange, UCSD

9:00 Art Feldman, Temple University – “The arginine vasopressin V1A receptor: a new target for heart failure therapy”

9:22 Burns Blaxall, Cincinnati Children’s Hospital – “ The role of PAR-1 in myocyte dysfunction”

9:44 Kika Sucharov, UC Denver – “Novel mechanisms of heart failure – lessons from the pediatric heart”

10:06 Michael Kapiloff, University of Miami – “RSK3 – a therapeutic target in heart failure” 9:00 AM - 10:30 AM – Interest Group 5 Session – Ischemia and Mitochondria Chairs: Chris Baines, University of Missouri and Randi Parks, NHLBI

9:00 David Lefer, Louisiana University School of Medicine – “CAESAR: A consortium for cardioprotection”

9:22 Xin-Liang Ma, Temple University – “Systemic adiponectin malfunction and ischemic heart failure”

9:44 Chris Baines, University fo Missouri – “The mitochondrial protein C1qbp binds to cyclophilin D and ATP synthase and regulates the mitochondrial permeability transition in the heart”

10:06 Charles Steenbergen, Johns Hopkins University – “MicroRNA in mitochondria” 10:30 AM - 11:00 AM – Coffee Break 11:00 AM – 12:30 PM – Interest Group 3 Session – Stem Cell and Gene Therapy Chairs: Farid Moussavi-Harami, University of Washington and Joseph Hill, UTSW

11:00 Joseph Wu, Stanford University – “iPSCs for cardiac disease modeling and drug discovery”

11:22 Julie Ritterhoff, University of Heidelberg – “S100A1 gene therapy for heart failure”

11:44 Li Qian, University of North Carolina – “Barriers to direct cardiac reprogramming”

12:06 Michael Chin, University of Washington – “Tafazzin enzyme replacement therapy in a mouse model of Barth Syndrome”

11:00AM - 12:30 PM – Interest Group 4: Ion Channels, E-C Coupling and Contractile Proteins Chairs: Chris Liu, University of Washington and Pieter DeTombe,University of Loyola

11:00 Wolfgang Linke, Ruhr University, Bochum: “Postranslational modifications of the giant protein titin in heart

5:30 PM 7:00 PM Posters & Reception - Session II (079-156) Princessa Ballroom and Foyer

5:30 PM 7:30 PM Satellite Session: NHLBI Proteomics Seminar at ISHR 2015

“Quantitative Proteomics: Applications to Cardiovascular Biology"

Eliza Anderson Amphitheater

7:00 PM 10:00 PM ECI Social (Ticket required) Tap House Grill 1506 6th Ave Seattle, WA 98101

7:30PM 10:00 PM Evening Entertainment

failure: new mechanistic insight and therapeutic implications”

11:22 Ye Chen-Izu, UC Davis: “Mechano-chemo-transduction during cardiomyocyte contraction via localized NOS signaling”

11:44 Xander Wehrens, Baylor College of Medicine: “Role of protein phosphatases in atrial fibrillation”

12:06 Litsa Kranias, University of Cincinnati: “Hsp20/protein phosphatase 1/SERCA regulatory axis in heart failure” 12:30 PM – 2:00 PM Lunch & Posters 2:00 PM - 3:30 PM – Interest Group 2 Session: Cardiac Metabolism Chairs: Lawrence Young, Yale University and Nathan Roe University of Washington

2:00 Ira Goldberg, Columbia University – “Understanding cardiac lipotoxicity”

2:22 Jun Ren, University of Wyoming – “Cardiac autophagy in obesity”

2:44 Qinghang Liu, University of Washington – Oral Abstract – “A critical TAK1 signaling pathway regulating programmed necrosis, myocardial remodeling, and heart failure propensity”

3:06 Dale Abel, University of Iowa – “Mechanisms of diabetic cardiomyopathy” 2:00 PM - 3:30 PM Session I: Hypertrophy and Cardiomyopathy Chairs: Joan Heller Brown, UC San Diego and and Chen Gao, UCLA

2:00 John Seidman, Harvard – “Deep sequencing to understand cardiomyopathy genotype and phenotype”

2:20 Jeff Towbin, Cincinnati Children’s Hospital – “Final common pathways of cardiomyopathies and overlapping phenotypes”

2:40 Kersten Small, Merc Research Labs, Kenilworth, NJ – Oral Abstract - “Filling the HFpEF Gap to Guide New Therapies - The Development of HFpEF Animal Models”

2:50 Jun Sadoshima, Rutgers University – “Maladaptive signaling in cardiomyopathy and failure”

3:10 Jenny van Eyk, Cedars-Sinai – “Cross talk between PTMs: the regulation of PKG and GSK 3beta pathways” 3:30 PM - 4:00 PM – Coffee Break 4:00 PM - 5:30 PM Session II: Cardiac Epigenetics Chairs: Matt Hartman, University of Washington and Kaytlyn Gerbin, University of Washington

4:00 Benoit Bruneau, Gladstone Institute – “Chromatin dynamics during cardiogenesis”

4:20 Dazhi Wang, Harvard University – “Non-coding RNAs in cardiac development and diseases”

4:40 Yiqiang Zhang, University of Washington – Oral Abstract - “Transcriptomic and Epigenomic Reprogramming of Adult Cardiomyocyte-Derived Cardiac Progenitor Cells”

4:50 John Stamatoyannopoulous, University of Washington – “Regulatory DNA elements that define cell history”

5:10 Robb MacLellan, University of Washington – “Epigenetic control of the cardiac cell cycle” 4:00PM - 5:30 PM Session III: Multi-Scale Modeling of the Heart Chairs: Mike Regnier, University of Washington and Enrique Guerrero, Mexico

4:00 Jil Tardiff, University of Arizona – “Cardiac thin filament models”

4:20 C. David Williams – “Multi-filament models of cardiac sarcomeres”

4:40 Jeffrey Erickson, University of Otago – Oral Abstract - “A novel mechanism for modulation of cardiac CaMKII activity by nitric oxide”

4:50 Dan Beard, University of Michigan – “The metabolic contribution to whole-organ mechanical dysfunction in heart failure”

5:10 Andrew McCulloch, UC San Diego – “Patient-specific multi-scale modeling of dissynchronous heart failure”

5:30 – 7:00 PM Reception and Posters (79-156) 5:30-7:30 Satellite Session: NHLBI Proteomics Seminar at ISHR 2015 “Quantitative Proteomics: Applications to Cardiovascular Biology" Amphitheater, Grant Hyatt Seattle. Seattle, WA Moderators: Lisa Schwartz-Longacre, NHLBI &Edward Lau, . University of California at Los Angeles 5:30 - 5:50 Ying Ge, University of Wisconsin-Madison Top-down Proteomics in Heart Failure: Novel Molecular Insights Enabled by Cutting-edge Technology 5:50 - 6:10 Mark Kohr The Johns Hopkins University Assessing Redox-sensitive Cysteine Modifications in the Myocardium 6:10 - 6:30 Maggie Lam University of California at Los Angeles Data Science of Proteomics: Prioritizing Development of High-demand Quantitative Assays for Cardiovascular Research 6:30 - 6:50 D Brian Foster The Johns Hopkins University Integrated Omic Analysis of Heart Failure Progression 6:50 - 7:10 Sarah Franklin University of Utah Remodeling of Histone Modifications in Cardiac Hypertrophy and Failure 7:10 to 7:30 Lisa Schwartz-Longacre, NHLBI Additional Q&A; Closing Remarks 7:30 - 10:00 PM Early Career Social Event; Others on their own to explore Seattle

Tuesday – June 9, 2015

Overview

8:00AM 5:00PM Registration Leonesa Foyer

8:00 AM 6:00 PM Speaker Ready Room Favorita

8:00 AM 8:45 AM Plenary Talk–Keith Reimer Lecture Leonesa I/II

9:00 AM 10:30 AM Session IV Eliza Anderson Amphitheater

9:00 AM 10:30 AM Session V Leonesa I/II

10:30 AM 10:45 AM Coffee Break Leonesa Foyer

10:45 AM 12:15 PM Session VI Leonesa I/II

10:45 AM 12:15 PM Session VII Eliza Anderson Amphitheater

12:15 PM 2:00 PM Lunch (on your own)

12:30 PM 1:30 PM ISHR – NAS Council Meeting Minzies Suite – 6th floor

2:00 PM 3:30 PM Session VIII Leonesa I/II

2:00 PM 3:30 PM Session IX Eliza Anderson Amphitheater

3:30 PM 3:45 PM Coffee Break Leonesa Foyer

3:45 PM 5:15 PM Session X Leonesa I/II

3:45 PM 5:15 PM Session XI Eliza Anderson Amphitheater

5:45 PM Board Buses Blake Island Cruise & Dinner

8:00 AM - 8:45AM Plenary Lecture Chair: Michael Regnier, PhD

Lee Hood, MD, PhD President & Co-Founder, Institute for Systems Biology

Member of the National Academy of Science Member of the National Academy of Engineering

Member of the Institute of Medicine Talk Title: “Proactive P4 Medicine: Catalyzing a Revolution in Healthcare though Wellness” 9:00 AM – 10:30 AM Session IV: Cardiovascular Bioengineering Chairs: Ying Zheng, University of Washington and Sakthivel Sadayappan, Loyola University

9:00 Beth Pruitt, Stanford – “Micromechanical systems to study force dynamics and mechanotransduction in cardiomyocytes”

9:20 Ying Zheng, University of Washington – “Engineering Human Organ-Specific Microcirculations”

9:40 Sumit Kar, University of Wisconsin – Oral Abstract – “Phosphorylation of cardiac myosin binding protein C is a dominant determinant of diastolic function in engineered cardiac tissue”

9:50 Farid Moussavi-Harami – Oral Abstract – “2-deoxy adenosine triphosphate improves contraction and length-dependent activation in human end-stage heart failure”

10:00 Deok-Ho Kim, University of Washington – “Cardiomyocyte maturation through nanopatterning” 9:00 AM – 10:30 AM Session V: Novel Regulatory Mechanisms of Cardiac Metabolism Chairs: Stephen Kolwicz, University of Washington and John Elrod, Temple

9:00 Tish Murphy, NIH – “Hypoxic signaling – beyond HIF”

9:20 Gary Lopaschuk, University of Alberta – “Protein acetylation and fatty acid oxidation”

9:40 Chi Fung Lee, University of Washington – Oral Abstract – “Restoration of NAD Redox Balance Ameliorates Heart Failure through Regulation of Cytosolic and Mitochondrial Protein Acetylation”

9:50 Wang Wang, University of Washington – “Beta adrenergic signaling on single mitochondria”

10:10 Doug Lewandowski, University of Illinois Chicago – “Regulation of endogenous lipid metabolism” Speaker chosen from the abstracts 10:30 AM – 10:45 AM – Coffee Break 10:45 AM - 12:15 PM Session VI: Stem Cells I: Basic and small animal studies Chairs: Eric Adler, UC San Diego and Catherine Passarrello, Univerity of Miami

10:45 Mike Laflamme, University of Washington – “Electrical integration of pluripotent stem cell-derived cardiac grafts”

11:05 Jop van Berlo, University of Minnesota – “Lineage tracing of adult cardiac stem cells”

11:25 Farid El-Sayed, San Diego State University – Oral Abstract - “Empowering Cardiac Progenitor Cell-Mediated Repair of Injured Myocardium by Overexpressing P2Y14 Nucleotide Receptor”

11:35 Lior Gepstein, Technion University – “Disease modeling with human induced pluripotent stem cells”

11:55 Loren Field, Indiana University – “Gene pathways controlling cardiomyocyte renewal” 10:45 AM - 12:15 PM Session VII: Ion Channels and Heart Disease Chairs: Susan Howlett, Dalhousie University and Chris Murray, University of BC

10:45 Fernando Santana, University of Washington – “Ca2+ handling dysfunction in heart failure”

11:03 Steve Houser, Temple University – “Altered Ca2+ regulation in pathological cardiac hypertrophy”

11:21 Bjorn Knollman, Vanderbilt University – “Ca2+ triggered arrhythmias”

11:39 Jeanne Nerbonne, Washington University in St. Louis – “Mechanisms contributing to electrical remodeling in hypertrophy and failure”

11:57 Bertil Hille, University of Washington – “Phosphoinositide lipids regulate ion channels and excitability” 12:15 PM – 2:00 PM Lunch 2:00 PM – 3:30 PM Session VIII: Metabolism/Metabolomics: cancer vs. heart failure Chairs: Heinrich Taegtmeyer, UT Houston

2:00 Mohit Jain, UC San Diego – “Targeting cancer metabolism for therapy”

2:20 Daniel Raftery, University of Washington – “Metabolite profiles: cancer vs. heart”

2:40 Maenjo Kim, University of Washington – Oral Abstract – “Genetic Deletion of the γ2-subunit of AMPK Aggravates Cardiac Dysfunction During Pathological Hypertrophy”

2:50 Daniel Kelly, Sanford-Burnham Medical Research Institute – “A systems approach to identify new targets in heart failure”

3:10 Peter Rabinovitch, University of Washington – “Extending cardiac healthspan by enhancing mitochondrial function” 2:00 PM - 3:30 PM Session IX: Proteotoxicity and Heart Failure Chairs: Loren Wold, Ohio State University and Tim O’Connell, University of Minnesota

2:00 Federica Del Monte, Harvard University – “Cofillin and protein aggregates in human heart failure”

2:20 X. J. Wang, University of South Dakota – “Protein degradation and heart failure”

2:40 Monte Willis, University of North Carolina – Oral Abstract – “MuRF1 inhibits Cardiac Thyroid Hormone Signaling by TRα Mono-Ubiquitination and Localization to CAP350”

2:50 Yibin Wang, UCLA – “LncRNA mediated regulation in cardiac hypertrophy”

3:10 Jeff Robbins, Cincinnati Children’s Research Institute – “Protein aggregates and autophagy: friend or foe” 3:30 PM - 3:45 PM Coffee Break 3:45 PM - 5:15 PM Session X: Cardiac Gene Therapy Chairs: Zejing Wang, Fred Hutchinson Cancer Research Center and Namakkal Soorappan, UAB

3:45 Wally Koch, Temple University - “GRK2 inhibition for heart failure”

4:05 Mike Regnier, University of Washington – “Ribonucleotide reductase gene therapy for heart failure”

4:25 Chi Keung Lam, University of Cinncinnati – Oral Abstract – “HAX-1 is a new regulator of cyclophilin-D expression and the mitochondria permeability transition pore in the heart”

4:35 Hee-Cheol Cho, Cedars-Sinai Medical Center – “Pacing the heart with genes and cells”

4:55 Jeff Chamberlain, University of Washington – “Dystrophin/utrophin gene therapy for muscular dystrophy” 3:45 PM – 5:15 PM Session XI: Cardiovascular Genomics Chairs: Sarah Franklin, University of Utah and Mike Tranter, University of Cincinatti

3:45 Tom Vondriska, UCLA – “Epigenomic control of the failing heart”

4:05 Dan Garry, University of Minnesota – “Systems approaches to understanding cardiovascular and blood development”

4:25 Christoph Rau, UCLA – Oral Abstract – “Network-based Approaches to Identify Novel Regulators of Heart Failure”

4:35 Junhui Sun, NIH/NHLBI – Oral Abstract – “Synergistic cardioprotection induced by pharmacologica postconditioning with HS and NO donors:S-sulfhydration (SSH) vs S-nitrosylation (SNO)

4:55 Cole Trapnell, University of Washington – “Genomics at the single-cell level”

5:45PM – Board Buses for Blake Island Cruise & Dinner Boat leaves at 6:45PM

Wednesday June 10, 2015

Overview

8:00AM 12:00PM Registration Leonesa Foyer

8:00 AM 12:00 PM Speaker Ready Room Favorita

8:00 AM 8:45 AM Plenary Lecture - Closing Leonesa I/II

9:00 AM 10:30 AM Session XII Leonesa I/II

9:00 AM 10:30 AM Session XIV Eliza Anderson Amphitheater

10:30 AM 11:00 AM Coffee Break Leonesa Foyer

11:00 AM 12:30 PM Session XV Leonesa I/II

11:00 AM 12:30 PM Session XIII Eliza Anderson Amphitheater

12:30 PM Closing Leonesa I/II

8:00 AM – 8:45 AM Plenary Talk – Keith Reimer Lecture Chairs: Elizabeth Murphy and Chuck Murry

Gerald Dorn, MD Philip & Sima K Needleman Professor of Medicine

Departments of Internal Medicine and Pharmacogenomics Division of Biology & Biomedical Sciences

Washington University in St. Louis Talk Title: “Ancestry vs Ambiance: The evolutionary underpinnings of cardiac hypertrophy signaling” 9:00 AM – 10:30 AM Session XII: Mitochondria and Cardioprotection Chairs: Rong Tian, University of Washington

9:00 Peipei Ping, UCLA – “Maintenance and turnover of the mitochondrial proteome”

9:20 Asa Gustafsson, UC San Diego – “Mitophagy and heart failure”

9:40 Moshi Song, Washington University, Saint Louis – Oral Abstract – “Interdependence of mitochondrial fission and mitophagy in adult mouse hearts”

9:50 Shey-shing Sheu, Jefferson University – “Dynamic mitochondrial Ca2+ and ROS signaling in cardiac muscle cells”

10:10 Robbie Gottlieb, Cedars Sinai Medical Center – “Mitophagy and biogenesis” 9:00 AM - 10:30 PM Session XIV: Stem Cells II: Large animal and clinical studies Chairs: April Stempien-Otero, University of Washington and Edward Lau, UCLA

9:00 Eduardo Marban, Cedars-Sinai – “Cardiosphere-derived cells: How they work and what they can do”

9:20 Joshua Hare, University of Miami – “Mesenchymal stem cells: How they work and what they can do”

9:40 Tromondae Feaster, Vanderbilt University – Oral Abstract – “Comparable calcium handling of human iPSC-derived cardiomyocytes generated by multiple laboratories”

9:50 Jay Zhang, Univ. of Minnesota – “Epicardial patches for heart repair: How they work and what they can do”

10:10 Chuck Murry, University of Washington – “Human cardiomyocytes: How they work and what they can do” 10:30 AM – 11:00 AM Coffee Break 11:00 AM - 12:30 AM Session XIII: Cardiac Myofilament Proteins Chairs: John Solaro, University of Illinois, Chicago and Glen Pyle, Univ of Guelph

11:00 Richard Moss, University of Wisconsin – “cMyBP-C & diastolic dysfunction in inherited and acquired cardiomyopathies”

11:20 J.P. Jin, Wayne State University – “Regulation & function of troponin in cardiac adaptation & heart failure”

11:40 Ilka Lorenzen-Schmidt, University of Guelph – Oral Abstract – “Crossing the line: A decrease in the Z-line protein CapZ worsens function after ischemia-reperfusion injury”

11:50 Glen Tibbits, Simon Frasier University – “Troponin and cardiomyopathies”

12:10 Henk Granzier, University of Arizona – “Titin and/or Nebulin and cardiomyopathies” 11:00 AM – 12:30 PM Session XV: Cardiac Signaling Pathways Chairs: Wang Wang, University of Washington and Cat Makarewich, UTSW

11:00 Jeff Molkentin, Cincinnati Children’s Research Institute – “Hypertrophic signaling”

11:20 Howard Rockman, Duke University – “GPCR signaling in the heart”

11:40 Joan Heller Brown, UCSD – Oral Abstract – “Selective coupling of the S1P3 receptor subtype to S1P-mediated RhoA activation and cardioprotection”

11:50 Ronglih Liao, Harvard University – “Amyloid cardiomyopathy”

12:10 Lorrie Kirshenbaum, University of Manitoba – “protective mechanisms against cell death” 12:30 PM Meeting Closed

POSTER SESSIONS Posters are located in the Princessa I/II Suite and lobby. Poster Session 1 Sunday, June 7 7:15-9:00 PM Posters P001 to P078 Poster Session 2 Monday, June 8 12:30-2:00 PM and 5:30-7:00 PM Posters P079 to P156 Presenting author underlined

P#

POSTER SESSION 1: Sunday, June 7, 2015 7:15-9:00 PM

001

Serum metabolomics reveal a distinct fingerprint of heart failure with preserved ejection fraction

Beshay Zordoky1, Miranda Sung1, Justin Ezekowitz1, Rupasri Mandal1, Beomsoo Han1, Trent Bjorndahl1, Souhaila Bouatra1, Todd Anderson2, Gavin Oudit1, David Wishart1, Jason Dyck1, 1University of Alberta, Edmonton, Alberta, Canada, 2University of Calgary, Calgary, Alberta, Canada

002

Cardiac ion channel changes in response to the ER stress Man Liu1, Guangbin Shi1, Hong Liu1, Samuel C. Dudley1, 1Rhode Island Hospital and Brown University, Providence, RI, USA

003

Cellular mechanisms of endogenous cardiomyocyte regeneration in injured hearts Yiqiang Zhang1, Yonggang Liu1, Avin Mehri1, Yi Lin1, Amy Martinson1, Michael Chin1, Charles Murry1, William MacLellan1, 1University of Washington, Seattle, WA, USA

004

Effect of SERCA inhibition on sarcoplasmic reticulum Ca alternans in intact rabbit hearts Lianguo Wang1, Rachel Myles2, Donald Bers1, Crystal Ripplinger1, 1UC Davis, Davis, CA, USA,

2University of Glasgow, Glasgow, UK

005

Synergistic cardioprotection induced by pharmacological postconditioning with H2S and NO donors:S-sulfhydration (SSH) vs S-nitrosylation (SNO) Junhui Sun1, Angel Aponte1, Sara Menazza1, Marjan Gucek1, Charles Steenbergen2, Elizabeth Murphy0, 1Systems Biology Center, NHLBI/NIH, Bethesda, MD, USA, 2Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD, USA

006

Proteomic approaches to identify the cardiac prolyl hydroxalome and to analyze protein stability Andrea Stoehr1, Alicia Evangelista1, Sajni Patel1, Yongshun Lin1, Angel Aponte1, Marjan Gucek1, Elizabeth Murphy1, 1NIH/NHLBI, Bethesda, MD, USA

007

The novel Cyclophilin-D interacting protein FASTKD1 protects cells against oxidative stress-induced death Kurt Marshall1, Maike Krenz2,3, Christopher Baines1,3, 1Department of Biomedical Sciences, University of Missouri-Columbia, Columbia, Missouri, USA, 2Department of Medical Pharmacology and Physiology, University of Missouri-Columbia, Columbia, Missouri, USA, 3Dalton Cardiovascular Research Center, University of Missouri-Columbia, Columbia, Missouri, USA

009

A human S10F-Hsp20 mutant abrogates the contractile and protective effects of Hsp20 in the heart Guan-Sheng Liu1, Wen Zhao1, Elizabeth Vafiadaki2, George Adly1, Chi Keung Lam1, Wen-Feng Cai3, Persoulla Nicolaou1, Min Jiang4, Jack Rubinstein4, Guo-Chang Fan1, Gerald W Dorn II5, Evangelia G Kranias1,2, 1Department of Pharmacology & Cell Biophysics, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA, 2Molecular Biology Division, Center for Basic Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece, Greece, 3Department of Pathology & Lab Medicine, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA, 4Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA, 5Center for Pharmacogenomics, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO, USA

010

HAX-1 is a new regulator of cyclophilin-D expression and the mitochondria permeability transition pore in the heart Chi Keung Lam1, Wen Zhao1, Guansheng Liu1, Wenfeng Cai1, George Gardner1, Evangelia Kranias1, 1University of Cincinnati, Cincinnati, OH, USA

011

Network-based approaches to identify novel regulators of heart failure Christoph Rau1, Milagros Romay1, Jessica Wang1, Shuxun Ren1, Yibin Wang1, Aldons Lusis1, 1University of California, Los Angeles, Los Angeles, CA, USA

012

Human cardiac, endothelial and blood lineages are controlled by gradients of activin A, BMP4, and Wnt/Β-catenin signaling Nathan Palpant1, Lil Pabon1, Meredith Roberts1, Brandon Hadland1, Daniel Jones1, Christina Jones1, Randall Moon1, Walter Ruzzo1, Irwin Bernstein1, Ying Zheng1, Charles Murry1, 1University of Washington, Seattle, WA, USA

013

Non-nuclear estrogen receptor activation reduces cardiac ischemic-reperfusion injury in mice with cardiac specific ablation of ER-alpha

Sara Menazza1, Swathi Appachi1, Junhui Sun1, Benita Katzenellenbogen2, John Katzenellenbogen3, Philip Shaul4, Elizabeth Murphy1, 1Systems Biology Center, NHLBI, NIH, Bethesda, MD, USA,

2Department of Molecular and Integrative Physiology, University of Illinois at Urbana-Champaign, Urbana, IL, USA, 3Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL, USA, 4Department of Pediatrics, Division of Pulmonary and Vascular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA

014

Recapitulating human myocardial aging and regeneration using wild mice Nirmala Hariharan1, Jacqueline Emathinger2, Nathalie Nguyen2, Pearl Quijada2, Hazel Salunga2, Donald Bers1, Mark Sussman2, 1University of California at Davis, Davis, CA, USA, 2San Diego State University, San Diego, CA, USA

015

Atrial-selective targeting of arrhythmogenic phase-3 early afterdepolarizations in human myocytes Stefano Morotti1, Andrew McCulloch2, Donald Bers1, Andrew Edwards3,4, Eleonora Grandi1, 1University of California Davis, Davis, Ca, USA, 2University of California San Diego, San Diego, Ca, USA, 3Oslo University Hospital, Oslo, Norway, 4Simula Research Laboratory, Lysaker, Norway

016

β-arrestin signaling as a novel therapeutic approach to familial dilated cardiomyopathy David M. Ryba1, Conrad L. Cowan2, David F. Wieczorek3, Beata M. Wolska1, R. John Solaro1, 1University of Illinois at Chicago, Chicago, IL, USA, 2Trevena, Inc., King of Prussia, PA, USA,

3University of Cincinnati, Cincinnati, OH, USA

017

Type 3 p90 ribosomal S6 kinase is required for concentric myocyte hypertrophy in a mouse model for Noonan syndrome Catherine L. Passariello1, Eliana C. Martinez1, Jinling Li1, Michael S. Kapiloff1, 1University of Miami, Miami, FL, USA

018

Cardiac myosin binding protein-C R495Q and R502W mutations slow contractile kinetics in an engineered cardiac tissue model Dan Smelter1, Sumit Kar1, Willem de Lange1, Carter Ralphe1, 1University of Wisconsin, Madison, WI, USA

019

Enhancing myocardial repair with cardiochimeras Pearl Quijada1, Hazel Salunga1, Nirmala Hariharan2, Farid El-Sayed1, Maryam Moshref1, Roberto Alvarez1, Natalie Gude1, Mark Sussman1, 1San Diego State University, San Diego, USA, 2University of California, Davis, Davis, USA

020

Enhanced Na+ - glucose cotransport causes Na+ overload in diabetic hearts Rebekah Lambert1, Sarah Srodulski1, Xiaoli Peng1, Kenneth Margulies2, Florin Despa1, Sanda Despa1, 1University of Kentucky, Lexington, KY, USA, 2University of Pennsylvania, Philadelphia, PA, USA

021

Gene delivery into rat myocardium using Cardiac Isoform of alpha-2 macroglobulin – a new Cardiac Biomarker (CA2M) Ponnambalam Annapoorani1, 1.V.V.Vanniaperumal College For Women, Virudhunagar, Tamil Nadu, India

022

Development of tissue engineered small diameter vascular grafts Harveen Lamba1, Yakov Elgudin1, Gary Wnek2, Steven Emancipator1, 1Louis Stokes Veterans Affairs, Cleveland, Ohio, USA, 2Case Western Reserve University School of Engineering, Cleveland, Ohio, USA

023

Reciprocal regulation of cardiac chromatin by HMGB and CTCF: implications for transcriptional regulation in pathologic hypertrophy Emma Monte1, Manuel Rosa-Garrido1, Elaheh Karbassi1, Haodong Chen1, Christoph Rau1, Jessica Wang1, Yong Wu1, Enrico Stefani1, James N. Weiss1, Aldons J. Lusis1, Siavash Kurdistani1, Sarah Franklin2, Thomas M. Vondriska1, 1UCLA, Los Angeles, CA, USA, 2Cardiovascular Research and Training Institute, University of Utah, Salt Lake City, UT, USA

024

TRPV2 stimulation diminishes peripartum cardiomyopathy through increased ejection fraction and decreased dilation.

Evan Onusko1, Guansheng Liu1, Sheryl Koch1, Min Jiang1, Evangelia Kranias1, Jack Rubinstein1, 1University of Cincinnati, Cincinnati, Ohio, USA

025

Phosphorylation of cardiac myosin binding protein C is a dominant determinant of diastolic function in engineered cardiac tissue Sumit Kar1, Dan Smelter1, Willem de Lange1, Daniel Fitzsimons1, Richard Moss1, J Carter Ralphe1, 1University of Wisconsin-Madison, Madison, WI, USA

026

CARD9 knockout reduces myocardial ischemia and reperfusion injury in mice Xing Qin1, Li Cao1, Tiantian Zheng1, Sreejayan Nair1, Jun Ren1, Guanglong He1, 1School of Pharmacy, University of Wyoming, Laramie, USA

027

Myocardial accumulation of amylin induces oxidative stress through sarcolemmal lipid peroxidation Miao Liu1, Nirmal Verma1, Xiao Li Peng1, Andrew Morris1, Kenneth Margulies2, Sanda Despa1, Florin Despa1, 1University of Kentucky, Lexington, KY, USA, 2University of Pennsylvania, Philadelphia, PA, USA

028

Role of miR-181 family in heart failure: a tale of two intracellular cardiomyocyte compartments Samarjit Das1, Mark Kohr1, Brittany Dunkerly1, Djahida Bedja1, Oliver Kent2, Anthony Leung1, Jorge Henao-Mejia53, Richard Flavell3, David Kass1, Charles Steenbergen1, 1Johns Hopkins University, Baltimore, MD, USA, 2University of Toronto, Toronto, ON, Canada, 3Yale University School of Medicine, New Haven, CT, USA

029

Mitochondrial Ca2+ uniporter inhibition induces unsolicited Ca2+ waves by increasing sarcoplasmic reticulum Ca2+ content Jesús Roberto Garza López1,2, Gerardo de Jesús García Rivas1,2, 1Tecnologico de Monterrey, Nuevo León, Mexico, 2Cátedra de Cardiología de la Escula de Medicina del Tecnológico de Monterrey, Nuevo León, Mexico

030

Chaer lncRNA negatively regulates PRC2 during cardiac hypertrophy Zhihua Wang1, Xinghua Wang2, Iris Chen1, Chen Gao1, Tomohiro Yokota1, He Wang1, Shuxun Ren1, Ashley Cass3, Xinshu Xiao3, Guangping Li2, Yibin Wang1, 1Division of Molecular Medicine, Departments of Anesthesiology, Physiology and Medicine, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, USA, 2Department of Cardiology, Tianjin Institute of Cardiology, Second Hospital of Tianjin Medical University, Tianjin, China, 3Molecular Biology Institute, Department of Integrative Biology and Physiology, College of Life Sciences, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, USA

031

Comparable calcium handling of human iPSC-derived cardiomyocytes generated by multiple laboratories Tromondae K. Feaster1, Hyun Seok Hwang1, Dmytro O. Kryshtal1, Verónica Sánchez-Freire2, Jianhua Zhang3, Timothy J. Kamp3, Charles C. Hong1, Joseph C. Wu2, Björn C. Knollmann0, 1Vanderbilt University School of Medicine, Nashville, TN, USA, 2Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA, 3Department of Cell and Regenerative Biology, School of Medicine and Public Health, University of Wisconsin, Madison, WI, USA

032

ImageStream analysis of dispersed human cardiomyocytes: a high through-put assay for proliferation Jun Luo1, Stephen Farris1, Deri Helterline1, April Stempien-Otero1, 1University of Washington, Seattle, WA, USA

033

Deletion of Diacylglycerol:acyltransferase 1 (DGAT1) selectively reduces incorporation of 16:0 fatty acid into triglyceride and alters cardiac metabolism Nathan Roe1, Rong Tian1, 1University of Washington, Seattle, WA, USA

034

Reduced autophagic flux contributes to accumulation of fragmented mitochondria in heart failure: impact of exercise training Juliane Campos1, Bruno Queliconi2, Luiz Bechara1, Paulo Jannig3, Paulo Dourado4, Kátia Gomes1, Vanessa Zambelli5, Cibele Resende6, Silvia Guatimosim6, Alicia Kowaltowski2, Daria Mochly-Rosen7, Patricia Brum3, Julio Ferreira1, 1Institute of Biomedical Sciences - University of Sao Paulo, Sao Paulo, SP, Brazil, 2Instituto de Química - Universidade de São Paulo, São Paulo, SP, Brazil, 3School of Physical Education and Sport - University of Sao Paulo, Sao Paulo, SP, Brazil, 4Heart Institute, Sao

Paulo, SP, Brazil, 5Butantan Institute, Sao Paulo, SP, Brazil, 6Physiology and Biophysics - Federal University of Minas Gerais, Belo Horizonte, MG, Brazil, 7Department of Chemical and Systems Biology - Stanford University School of Medicine, Stanford, CA, USA

035

The E487K variant of aldehyde dehydrogenase 2 protects cardiac mitochondrial metabolism in heart failure in mice Vanessa Lima1, Ivson Silva1, Cintia Ueta1, Julio Ferreira1, 1University of São Paulo, São Paulo, São Paulo, Brazil

036

Determining IKS β-subunit stoichiometry using unnatural amino acid mutagenesis Christopher Murray1, Maatje Westhoff1, Robert Emes1, Jodene Eldstrom1, David Fedida1, 1University of British Columbia, Vancouver, BC, Canada

037

"Silencing of the mitochondrial calcium uniporter improves post-ischemic cardiac dysfunction and attenuates mitochondrial Ca2+ overload and apoptosis in cardiac myoblast and cardiomyocytes". Yuriana Oropeza-Almazán1, Alberto Marbán-González1, Eduardo Reyes-Alvarez1, Gerardo García-Rivas1, 1Tecnológico de Monterrey, Monterrey, Mexico

038

Nrf2 dependent rescue of protein aggregation mediated hypertrophic cardiomyopathy Madhusudhanan Narasimhan1, Cory Miller2, Sakthivel Ramasamy1, Jennifer Hong2, Asokan Devarajan3, Nancy Atieno2, Christopher Davidson2, Kevin Whitehead2, John R. Hoidal2, Namakkal S Rajasekaran1, 1University of Alabama at Birmingham, Birmingham, USA, 2University of Utah, Salt Lake City, USA, 3University of California at LA, Las Angeles, USA

039

Interdependence of mitochondrial fission and mitophagy in adult mouse hearts Moshi Song1, Yan Burelle2, Gerald Dorn1, 1Washington University School of Medicine, Saint Louis, MO, USA, 2University of Montreal, Montréal, QC, Canada

040

Alternative splicing of NOX4 in the failing human heart Zoltán Varga1,2, Márton Pipicz3, Przemyslaw Leszek4, Júlia A. Baan5, Luca Mendler5,6, Tamás Baranyai1, Enrique Lara-Pezzi7, Pál Pacher2, Tamás Csont3, Péter Ferdinandy1,8, 1Cardiometabolic Research Group, Dept. of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, Hungary, 2Laboratory of Physiological Studies, National Institute on Alcohol Abuse and Alcoholism, National Institute of Health, Bethesda, Maryland, USA, 3Metabolic Diseases and Cell Signaling Group, Dept. of Biochemistry, University of Szeged, Szeged, Hungary, 4Institute of Cardiology, Warszawa, Poland, 5Muscle Adaptation Group, Dept. of Biochemistry, University of Szeged, Szeged, Hungary,

6nstitute of Biochemistry II, Goethe University, Faculty of Medicine, Frankfurt, Germany, 7Centro de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain, 8Pharmahungary Group, Szeged, Hungary

041

Novel roles for Chromogranin A peptide catestatin in cardiac metabolism and physiology Jan M. Schilling1,2, Teresa Pasqua2, Venkat R. Chirasani3, Nilima Biswas2, Ennio Avolio2, Hongqiang Cheng4, Suvajit Sen5, Alice Zemljic-Harpf1,2, John P. Headrick6, Heidi N. Fridolfsson1,2, Adam Kassan1,2, Kevin Yei7, Sumana Mahata7, Gautam Bandyopadhyay2, David M. Roth1,2, Nicholas J. G. Webster1,2, Sanjib Senapati3, Hemal H. Patel1,2, Sushil K. Mahata1,2, 1VASDHS, San Diego, CA, USA,

2UCSD, La Jolla, CA, USA, 3India Institute of Technology, Chennai, India, 4Zhejiang University School of Medicine, Hangzhou, China, 5UCLA, Los Angeles, CA, USA, 6Griffith Health Institute, Griffith, Australia, 7California Institue of Technology, Passadena, CA, USA

042

The phosphodiesterase-5 inhibitor vardenafil protects against diabetic cardiomyopathy in a type-2 diabetic animal model Csaba Mátyás1, Balázs Tamás Németh1, Attila Oláh1, Mihály Ruppert1, Dalma Kellermayer1, Marianna Török1, Lilla Szabó1, Gábor Szabó2, Béla Merkely1, Tamás Radovits1, 1Heart and Vascular Center, Semmelweis University, Budapest, Hungary, 2Department of Cardiac Surgery, University of Heidelberg, Heidelberg, Germany

043

MuRF1 inhibits cardiac thyroid hormone signaling by TRα mono-ubiquitination and localization to CAP350 Monte Willis1, Jessica Berthiaume2, Kristene Wadosky1, Wei Tang1, Martin Gerdes4, Michael Portman3, 1University of North Carolina, Chapel Hill, NC, USA, 2Case Western Reserve University, Cleveland, OH,, USA, 3Seattle Children’s Research Institute, University of Washington, Seattle, WA,

USA, 4New York College of Osteopathic Medicine at, New York Institute of Technology, Old Westbury, NY, USA

044

In utero exposure to diesel exhaust promotes cardiac fibrosis through enhanced cardiac myocyte apoptosis Jamie Goodson1, Chad Weldy2, Yonggang Liu1, Jesse Tsai1, James MacDonald1, Theo Bammler1, Michael Chin1, 1University of Washington, Seattle, WA, USA, 2Duke University, Durham, NC, USA

045

Chamber specific function of p38 MAP kinase during early postnatal development Tomohiro Yokota1, Vincent Ren1, Susumu Minamisawa2, Yibin Wang1, 1Department of Anesthesiology, Medicine and Physiology, University of California, Los Angeles, Los Angeles, CA, USA, 22) Department of Cell Physiology, Jikei University School of Medicine, Tokyo, Japan

046

Restoration of NAD redox balance ameliorates heart failure through regulation of cytosolic and mitochondrial protein acetylation Chi Fung Lee1, Lorena Garcia-Menendez1, Yongseon Choi1, Juan Chavez1, Jim Bruce1, Rong Tian1, 1University of Washington, Seattle,WA, USA

047

Transient mitochondrial permeability transition pore opening in cardiac myocytes during SR Ca release Xiyuan Lu1, Donald Bers1, 1university of california, davis, davis, USA

048

Characterization of mitochondria from mice lacking the mitochondrial calcium uniporter Randi Parks1, Sara Menazza1, Angel Aponte2, Kira Holmstrom3, Toren Finkel3, Elizabeth Murphy1, 1Systems Biology Center, Bethesda, MD, USA, 2Proteomic Core Facility, Bethesda, MD, USA, 3Center for Molecular Medicine, Bethesda, MD, USA

049

Mitochondrial complex II is a source of the reserve respiratory capacity that is regulated by metabolic sensors via sirtuin 3 Jessica Pfleger1, Minzhen He1, Maha Abdellatif1, 1Rutgers University, Newark, NJ, USA

050

A novel PKCalpha isoform in signaling for cardiac hypertrophy Chen Gao1, Jianli Gong3, William Wang3, Susan Steinberg3, Yibin Wang1,2, 1Division of Molecular Medicine, University of California, Los Angeles, Los Angeles, CA, USA, 2Molecular Biology Institute, University of California,Los Angeles, Los Angeles,CA, USA, 3Department of Pharmacology, Columbia University, New York, NY, USA

051

Ablation of Sirt5 in heart alters cardiac metabolism and increase ischemia-reperfusion injury. Jennifer Boylston1, Yong Chen2, Marjan Gucek2, Michael Sack3, Junhui Sun1, Elizabeth Murphy1, 1Sytems Biology Center, NHLBI, NIH, Bethesda, MD, USA, 2Proteomics Core, NHLBI, NIH, Bethesda, MD, USA, 3Center for Molecular Medicine, NHLBI, NIH, Betheday, MD, USA

052

Empowering cardiac progenitor cell-mediated repair of injured myocardium by overexpressing P2Y14 nucleotide receptor Farid El-Sayed1, Mark Sussman1, 1San Diego State University, San Diego, California, USA

053

PKCepsilon is required in physical exercise-mediated cardioprotection Lais Domingues1, Cintia B. Ueta1, Julio C.B. Ferreira1, 1University of São Paulo, São Paulo, SP, Brazil

054

Reduced cardiac hypertrophy observed in females is unaffected by the ablation of cardiac-specific ERα Josephine Harrington1,2, Sara Menazza1, Elizabeth Murphy1, 1NHLBI, National Institutes of Health, Bethesda, MD, USA, 2University of Massachusetts Medical School, Worcester, MA, USA

055

CARD9 knockout ameliorates obesity-associated cardiac dysfunction Li Cao1, Xing Qin1, Tiantian Zheng1, Sreejayan Nair1, Jun Ren1, Guanglong He1, 1School of Pharmacy, University of Wyoming, Laramie, Wyoming, USA

056

Molecular mechanisms underlie muramyl dipeptide-induced inflammation and autophagy in macrophages Tiantian Zheng1, Li Cao1, Xing Qin1, Sreejayan Nair1, Jun Ren1, Guanglong He1, 1University of Wyoming, Laramie, USA

057

Ethanol mediated-cardioprotection is lost in mice carrying the E487K variant of aldehyde dehydrogenase 2: benefits of Alda-1 Cintia B. Ueta1, Marie-Helene Disatnik2, Che-Hong Chen2, Daria Mochly-Rosen2, Julio C.B. Ferreira1, 1University of São Paulo, São Paulo, SP, Brazil, 2Stanford University, Stanford, CA, USA

058

S107 improves RBM20 deficiency-induced cardiac dysfunction Zhiyong Yin1,2, Chaoqun Zhu1, Jun Ren3, Wei Guo1,3, 1Animal Science, University of Wyoming, Laramie, WY, USA, 2Department of Cardiology, Xi Jing Hospital, Fourth Military Medical University, Xi'an, China, 3Center for Cardiovascular Research and Alternative Medicine, College of Health Science, University of Wyoming,, Laramie, WY, USA

059

A high-content RNAi screen for novel effectors of cardiac proteotoxicity Patrick McLendon1, Gregory Davis1, James Gulick1, Kritton Shay-Winkler1, Jeffery Molkentin1, Jeffrey Robbins1, 1Cincinnati Childrens Hospital Medical Center, Cincinnati, OH, USA

060

Genetic deletion of the γ2-subunit of AMPK aggravates cardiac dysfunction during pathological hypertrophy Maengjo Kim1, Lorena Garcia-Menendez1, Yong Seon Choi1, Richard Palmiter1, Rong Tian1, 1University of Washington, Seattle, WA, USA

061

An alpha-1A adrenergic receptor agonist to treat heart failure Megan D. Montgomery1,2, Trevor Chan1,2, Rajesh Dash1,2, Philip M. Swigart1,2, Bat-Erdene Myagmar1,2, Anthony J. Baker1,2, Paul C. Simpson1,2, 1University of California, San Francisco, San Francisco, CA, USA, 2San Francisco VA Medical Center, San Francisco, CA, USA

062

The mitochondrial protein C1qbp binds to cyclophilin D and ATP synthase and regulates the mitochondrial permeability transition in the heart Manuel Gutierrez-Aguilar1, Christopher Baines1, 1University of Missouri-Columbia, Columbia, MO, USA

063

FK 506 binding proteins facilitate the termination of Ca2+ release in wild type cardiac ryanodine receptor (RyR2), but not catecholaminergic polymorphic ventricular tachycardia (CPVT) associated RyR2 mutants Joe Zhang1, Helen Waddell1, Ella Wu1, Jhanvi Dholakia1, Janet McLay1, Peter Jones1, 1Deaprtment

of Physiology and HeartOtago, University of Otago, Otago, New Zealand

064

Toll-like receptor 4 knockout alleviates paraquat-induced cardiomyocyte contractile dysfunction through an autophagy-dependent mechanism Shuyi Wang1, Maolong Dong1, Jun Ren1, 1Center for Cardiovascular Research and Alternative Medicine, University of Wyoming College of Health Sciences, laramie,wyoming, USA

065

Impaired contractility and size-dependent toxicity by silica nanoparticles in adult rat cardiomyocytes Carlos Enrique Guerrero-Beltrán1, Jesús Roberto-Garza1, Alejandra García-García2, Noemí García1, Judith Bernal-Ramírez1, Nancy Ornelas1, Omar Lozano3, Elvin Blanco4, Gerardo García-Rivas1, 1Tecnológico de Monterrey, Monterrey, Nuevo León, Mexico, 2Centro de Investigación en Materiales Avanzados S.C., Apodaca, Nuevo León, Mexico, 3Université de Namur ASBL, Namur, Belgium, 4The Methodist Hospital Research Institute, Houston ,Texas, USA

066

G3BP1 differentially regulates two cardiac-enriched, non-coding RNAs and promotes hypertrophy development Minzhen He1, Danish Sayed1, 1Rutgers New Jersey Medical School, Newark, New Jersey, USA

067

Increasing myocardial fatty acid oxidation protects against pathological hypertrophy and diastolic dysfunction in mice Stephen Kolwicz1, Yong Seon Choi1, Ana Mattos1, Dan Shao1, Tao Li1, Eric Smith1, Miranda Nabben1, Wang Wang1, Rong Tian1, 1University of Washington, Seattle, WA, USA

068

Pathophysiological impact of a highly prevalent MYBPC3 gene variant causing hypertrophic cardiomyopathy in vivo Diederik Kuster1,2, David Barefield1, Suresh Govindan1, Mayandi Sivaguru3, Ben August4, Kyounghwan Lee5, Rajasekaran Namakkal-Soorappan6, Roger Craig5, Sakthivel Sadayappan1, 1Loyola University Chicago, Maywood, IL, USA, 2VU University Medical Center, Amsterdam, The Netherlands, 3University of Illinois at Urbana Champaign, Urbana, IL, USA,

4University of Wisconsin-Madison, Madison, WI, USA, 5University of Massachusetts Medical School, Worcester, MA, USA, 6University Alabama at Birmingham, Birmingham, AL, USA

069

Age-dependent cardiac contractile dysfunction is graded by frailty, not age, in senescent C57BL/6J mice Hirad Feridooni1, Arash Boroumandi2, Nazari Polidovitch3, Robert Rose1, Robert Tsushima2, Susan Howlett1, 1Dalhousie University, Halifax, Canada, 2York University, Tornoto, Canada, 3University of Toronto, Toronto, Canada

070

Diabetic hyperglycemia acutely affects action potentials and ionic currents through CaMKII activation on murine and rabbit ventricular myocytes Zhandi Liao1, Donald Bers1, 1University of California Davis, Davis, CA, USA

071

Role of CaMKII in cardiac frequency adaptation Tamas Banyasz1,2, Balazs Horvath1,2, Bence Hegyi1,2, Peter P. Nanasi1, Zhong Jian2, Rafael Shimkunas2, Leighton T. Izu2, Ye Chen-Izu2, 1Department of Physiology, University of Debrecen, Debrecen, Hungary, 2Department of Pharmacology, University of California Davis, Davis, CA, USA

072

Enhanced myocardial repair with CardioClusters Megan Monsanto1, Kristina Fisher1, Kevin White1, Natalie Gude1, Mark A. Sussman1, 1San Diego State University, San Diego, CA, USA

073

ALPK2 is a novel regulatory kinase required for heart development Peter Hofsteen1, Aaron Robitaille1, Nathan Palpant1, Lil Pabon1, Randall Moon1,2, Charles Murry1, 1University of Washington, Seattle, WA, USA, 2Howard Hughes Medical Institute, Seattle, WA, USA

074

Autophagy induction using rapamycin rescues against targeted deletion of PTEN in cardiomyocytes-induced cardiac contractile dysfunction Xiyao Chen1,2, Xiaoling Zhu3, Xiaoming Wang2, Jun Ren1, 1University of Wyoming College of Health Sciences, laramie, wyoming, USA, 2Departments of Geriatrics, Xijing Hospital, the Fourth Military Medical University, Xi'an, China, 3Departments of Anesthesiology, Xijing Hospital, the Fourth Military Medical University, Xi'an, China

075

PTP1B knockout rescues against ER stress-induced myocardial contractile dysfunction: Role of autophagy. Xiyao Chen1,2, Nair Sreejayan2, Xiaoming Wang1, Jun Ren2, 1Departments of Geriatrics, Xijing Hospital, the Fourth Military Medical University, Xi'an, China, 2Center for Cardiovascular Research and Alternative Medicine, University of Wyoming College of Health Sciences, Laramie, laramie, Wyoming, USA

076

Defective branched-chain amino acids catabolism induces metabolic remodeling and exacerbates ischemia/reperfusion injury in heart Tao Li1, Zhen Zhang1, Stephen C Kolwicz, Jr.1, Haipeng Sun2, Yibin Wang2, Rong Tian1, 1Mitochondria and Metabolism Center, Department of Anesthesiology and Pain Medicine, University of Washington, Seattle, WA, USA, 2Department of Anesthesiology, UCLA David Geffen School of Medicine, Los Angeles, CA, USA

077

Inhibition of late Na+ current as a therapeutic strategy for treating long QT syndrome Bence Hegyi1, Zhong Jian1, Rafael Shimkunas1, Leighton T Izu1, Sridharan Rajamani2, Luiz Belardinelli2, Ye Chen-Izu1, Tamas Banyasz1, 1Department of Pharmacology, University of California, Davis, Davis, CA, USA, 2Gilead Sciences Inc., Fremont, CA, USA

078

Mechano-chemo-transduction in cardiomyocytes contracting under mechanical load Rafael Shimkunas1,2, Zhong Jian2, Bence Hegyi2, John Shaw5, Nipavan Chiamvimonvat4, Kit S. Lam3, Kenneth Ginsburg2, Julie Bossuyt2, Donald M. Bers2, Leighton T. Izu2, Ye Chen-Izu1,2, 1Department of Biomedical Engineering, University of California, Davis, Davis, CA, USA, 2Department of Pharmacology, University of California, Davis, Davis, CA, USA, 3Department of Biochemistry and Molecular Medicine, University of California, Davis, Sacramento, CA, USA, 4Department of Internal Medicine/Cardiology, University of California, Davis, Sacramento, CA, USA, 5Department of Aerospace Engineering, University of Michigan, Ann Arbor, MI, USA

P# POSTER SESSION 2: Monday, June 8, 2015 12:30-2:00 PM and 5:30-7:00 PM 079

Fatty Acid Feeding Promotes the Maturation of Cardiomyocytes Derived from Human Pluripotent Stem Cells Xiulan Yang1, Marita Rodriguez1, Andrea Leonard1, Karin Fischer1, Lil Pabon1, Hans Reinecke1, Nathan Sniadecki1, Charles Murry1, 1University of Washington, Seattle WA, USA

080

Mechanistic studies of myosin light chain mutations associated with dilated, hypertrophic and restrictive cardiomyopathy Chen-Ching Yuan1, Wenrui Huang1, Jingsheng Liang1, Katarzyna Kazmierczak1, Ana I Rojas1, Rosemeire Kanashiro-Takeuchi1, Danuta Szczesna-Cordary1, 1University of Miami, Miller school of medicine, Miami,FL, USA

081

β1-AR /CaMKII Signaling Cause Cardiac Myocyte Death via Mitochondrial Calcium Overload Pei Wang1, Shengchen Xu1, Wang Wang1, 1Mitochondria and Metabolism Center, University of Washington, Seattle, WA, USA

082

Mitochondrial Aldehyde Dehydrogenase 2 (ALDH2) Deficiency Impairs Revascularization in Chronic Ischemia and Contributed to Poor Coronary Artery Collateral Circulation Xiangwei Liu1,3, Xiaolei Sun1,2, Zhen Dong1, Peng Wang1, Hong Zhu1, Aijun Sun1,2, Jun Ren1,3, 1Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China, 2Institute of Biomedical Science, Fudan University, Shanghai 200032, China, 3Center for Cardiovascular Research and Alternative Medicine, School of Pharmacy, University of Wyoming College of Health Sciences, Laramie, Wyoming, USA

083

Oxidative stress regulates titin elasticity by affecting Ig-domain stability Nazha Hamdani1, Jorge Alegre-Cebollada2, Martin Breitkreuz1, Lars Leichert3, Julio M. Fernandez3, Wolfgang A. Linke1, 1Dept. of Cardiovascular Physiology, Ruhr University, Bochum, Germany, Bochum, Germany, 2Columbia University,, New York, USA, 3Medical Proteome Center, Ruhr University, Bochum, Bochum, Germany

084

Endogenous DRP1 modulates cardiac respiration through mPTP and independent of fission Huiliang Zhang1, Sara Bisetto2, Shey-Shing Sheu2, Wang Wang1, 1Mitochondria and Metabolism Center, Department of Anesthesiology and Pain Medicine, University of Washington, Seattle, WA, USA, 2Center for Translational Medicine, Department of Medicine, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA, USA

085

Vascular stiffening precedes the onset of HFpEF in diabetics with diastolic dysfunction Ilya Karagodin1, Omer Aba-Omer1, Rodney Sparapani1, Jennifer Strande1, 1Medical College of Wisconsin, Milwaukee, WI, USA

086

Chronic testosterone withdrawal slows calcium transient decay and prolongs contraction in ventricular myocytes isolated from gonadectomised C57BL/6 male mice Omar Ayaz1, Susan Howlett1, Robert Rose1, 1Dalhousie University, Nova Scotia, Canada

087

Defining the in vivo consequences of altered interactions between cMyBP-C and actin on cardiac function in transgenic mice Sabine van Dijk1, Kristina Bezold2, Samantha Harris1, 1University of Arizona, Tucson, AZ, USA,

2Stanford University, Stanford, CA, USA

088

Notch-mediated proliferation of human embryonic stem cell-derived cardiomyocytes Kaytlyn A. Gerbin1, Charles E. Murry1, 1University of Washington, Seattle, WA, USA

089

Development of an intracellular enzyme replacement therapy for Barth Syndrome Ana Dinca1, Wei Ming Chien1, Michael Chin1, 1University of Washington, Seattle, USA

090

S100A1 DNA-based inotropic therapy protects against pro-arrhythmogenic ryanodine receptor 2 dysfunction Julia Ritterhoff1, Andreas Seitz1, Hugo A. Katus1,2, Patrick Most1,4, 1University Hopsital Heidelberg, Heidelberg, Germany, 2German Centre for Cardiovascular Research, Heidelberg/ Mannheim, Germany, 3Thomas Jefferson University, Philadelphia, PA, USA, 4uniQure GmbH Germany, Heidelberg, Germany

091

Gender-based differences in myocardial protein S-nitrosylation Qin Shao1, Elizabeth Murphy2, Charles Steenbergen1, Mark Kohr1,3, 1Johns Hopkins University School of Medicine, Baltimore, MD, USA, 2National Heart, Lung and Blood Institute, Bethesda, MD, USA, 3Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA

092

The COP9 Signalosome controls the Degradation of cytosolic misfolded Proteins and protects against cardiac Proteotoxicity Huabo Su1,2, Jie Li1,2, Hanming Zhang1, Ning Wei3, Xuejun Wang1, 1University of South Dakota, Vermillion, South Dakota, USA, 2Georgia Regents University, Augusta, Georgia, USA, 3Yale Univeristy, New Haven, Connecticut, USA

093

Coordinated Protein Turnover of Cardiac Metabolic Clusters in Hypertrophy Edward Lau1, Quan Cao1, Dominic Ng1, Ding Wang1, Maggie PY Lam1, Peipei Ping1, 1University of California at Los Angeles, Los Angeles, USA

094

Data science on proteomics: prioritizing development of high-demand quantitative protein assays for cardiovascular research Maggie PY Lam1, Vidya Venkatraman2,3, T. Umut Dincer1, Edward Lau1, Peipei Ping1, Jennifer Van Eyk2, 1University of California at Los Angeles, CA, USA, 2Cedars-Sinai Medical Center, CA, USA,

3Johns Hopkins University, MD, USA

095

The impact of acute exposure of progesterone on mechanisms of cardiac excitation-contraction coupling in isolated murine ventricular myocytes Jennifer MacDonald1, Hirad Feridooni1, Glen Pyle2, Susan Howlett1, 1Dalhousie University, Halifax, Canada, 2University of Guelph, Guelph, Canada

096

Regulation of cell cycle genes in neonatal mouse heart regeneration HIDEMI KAJIMOTO1, Kyohei Oyama1, W. Robb MacLellan1, 1University of Washington, Seattle, USA

097

2-Deoxy Adenosine Triphosphate Restores the Contractile Function of Cardiac Myofibril from Adult Dogs with Naturally Occurring Dilated Cardiomyopathy Yuanhua Cheng1, Kaley A Hogarth2, M Lynne O'Sullivan2, Michael Regnier1, W. Glen Pyle2, 1University of Washington, Seattle, WA, USA, 2University of Guelph, Guelph, Ontario, Canada

098

Investigating the pathogenesis of Δ160E mutation-linked Hypertrophic Cardiomyopathy Salwa Abdullah1, Mark McConnell1, Jil Tardiff1, 1University of Arizona, Tucson, Arizona, USA

099

Rise of diastolic Ca2+ explains the tachy-brady sinus node arrhythmia of Na+/Ca2+ exchange KO mice. Angelo G. Torrente1, Audrey Zaini1, Rui Zhang1, Jeanney Kang1, Kenneth D. Philipson2, Joshua I. Goldhaber1, 1Heart Institute Cedars Sinai, Los Angeles, CA, USA, 2David Geffen School of Medicine at UCLA, Los Angeles, CA, USA

100

Hyperactive mitochondrial dynamics mediates obesity-induced heart dysfunction Eric Smith1, Nicolas Gutierrez Cortes1, Huiliang Zhang1, Shangcheng Xu1, Pei Wang1, Wang Wang1, 1University of Washington School of Medicine; Mitochondria and Metabolism Center, Seattle, Washington, USA

101

High saturated fat diets alter endogenous cardiac lipid profiles in mice Loreta C. Tomasi1, Stephen C. Kolwicz1, 1Mitochondria & Metabolism Center, Department of Anesthesiology and Pain Medicine, University of Washington, Seattle, WA, USA

102

Postnatal hyperplasia precedes hypertrophy in mice lacking myosin binding protein C (cMyBP-C) - a model of hypertrophic cardiomyopathy (HCM) Adrian Grimes1, Emily Farrell2, Willem de Lange2, J. Carter Ralphe2, 1University of Wisconsin School of Medicine and Public Health, Department of Medicine, Madison, WI, USA, 2University of Wisconsin School of Medicine and Public Health, Department of Pediatrics, Madison, WI, USA

103

High glucose Suppresses Branched-chain Amino Acid Catabolism in the heart through downregulation of Kruppel-Like Factor 15 Dan Shao1, Zhen Zhang1, Sung Won Choi1, Haiwei Gu1, Danijel Djukovic1, Daniel Raftery1, Rong Tian1, 1Mitochondria and Metabolism Center, Department of Anesthesiology & Pain Medicine, University of Washington, Seattle, WA, USA

104

Altered Ca2+ binding properties of Hypertrophic Cardiomyopathy-related cardiac troponin T mutation Yueh Alison Li1, Bo Liang1,2, Kaveh Rayani1, Glen Tibbits1,2, 1Simon Fraser University, Burnaby, BC, Canada, 2Child and Family Research Institute, Vancouver, BC, Canada

105

Characterization of Human Induced Pluripotent Stem Cells-derived Cardiomyocytes by simultaneous voltage and calcium optical mapping Sanam Shafaattalab1, Eric Lin1, Christine Genge1, Glen Tibbits1,2, 1Simon Fraser University, Burnaby, Canada, 2Child and Family Research Institute, Vancouver, Canada

106

Isothermal Titration Calorimetry (ITC) derived thermodynamic analysis of calcium binding to recombinant human Troponin C (TnC) with Familial Hypertrophic Cardiomyopathy (FHC) associated mutations Kaveh Rayani1, Charles Stevens1,2, Alison Li1, Glen Tibbits1,2, 1Simon Fraser University, Burnaby, British Columbia, Canada, 2Child and Family Research Institute, Vancouver, British Columbia, Canada

107

Predicted structural and functional effects of cardiac troponin mutations associated with familial hypertrophic cardiomyopathy Charles Stevens1,2, Kaveh Rayani1, Glen Tibbits1,2, 1Simon Fraser University, Burnaby, BC, Canada,

2Child & Family Research Institute, Vancouver, BC, Canada

108

Post-Surgical Atrioventricular Nodal Arrhythmogenesis In The Neonate Heart Cici Chenliu1,2, Pauline Dan1,2, Yang Qu1,2, Eric Lin1,2, Shubhayan Sanatani2, Xiao Ye Sheng1,2, Glen Tibbits1,2, 1Simon Fraser University, Burnaby, BC, Canada, 2Child and Family Research Institute, Vancouver, BC, Canada

109

Comparison of the development and progression of cardiac dysfunction in various mouse models of metabolic stress Vongai Nyamandi1, Marysol Garcia-Patino1, Julia Nogueira Varela1, Kathleen MacLeod1, 1University of British Columbia, Vancouver, BC, Canada

110

Mice with heterozygous deletion of ROCK2 are protected against myocardial ischemia-reperfusion injury Marysol Garcia-Patino1, Vongai Nyamandi1, Julia Nogueira Varela1, Zhengping Jia2, Kathleen MacLeod1, 1Molecular and Cellular Pharmacology Research Group, Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, BC, Canada, 2Neurosciences & Mental Health, the Hospital for Sick Children, and Department of Physiology, Faculty of Medicine, University of Toronto, Toronto, ON, Canada

111

2-deoxy-ADP as a substrate for oxidative phosphorylation and creatine kinase Jason Murray1, Farid Moussavi-Harami1, David Marcinek1, Michael Regnier1, 1University of Washington, Seattle, Washington, USA

112

Rapamycin induces mitochondrial remodeling to rejuvenate energy metabolism and energetics in old hearts Ying Ann Chiao1, Stephen Kolwicz1, Nathan Basisty1, Michael MacCoss1, Rong Tian1, Peter Rabinovitch1, 1University of Washington, Seattle, WA, USA

113

CD29/Beta-1 integrin identifies and contributes to pathologic cardiac fibrosis. Stephen Farris1, Deri Helterline1, April Stempien-Otero1, 1University of Washington, Seattle, WA, USA

114

Sinoatrial node dysfunction in aged and frail mice assessed using high resolution optical mapping Motahareh Moghtadaei1, Sara Rafferty1, Susan Howlett1, Robert Rose1, 1Dalhousie University, Halifax, Nova Scotia, Canada

115

Assessment of sinoatrial node activity and atrial conduction as a function of age and frailty in mice Hailey Jansen1, Sara Rafferty1, Susan Howlett1, Robert Rose1, 1Dalhousie University, Halifax, Nova Scotia, Canada

116

Distinct effects of wildtype and mutant forms of atrial natriuretic peptide on atrial electrophysiology in mice and humans

Iuliia Polina1, Rui Hua1, Sarah MacLeod1, Motahareh Moghtadaei1, John Sapp1, Jean Francois Legare1, Stacy O'Blenes1, Robert Rose1, 1Dalhousie University, Halifax, Nova Scotia, Canada

117

Muscle Ring Finger-1 (MuRF1) Enhances Autophagic Flux In vivo Traci Parry1, Megan Quintana1, Joseph Hill2, Monte Willis1, 1University of North Carolina, Chapel Hill, NC, USA, 2University of Texas Southwestern, Dallas, TX, USA

118

Calcineurin and protein phosphatase 2A modulate cardiac gap junction conductance in guinea-pig left atrium: Role of Cx43 and Cx40 phosphorylation state Fiona Hatch1, Samantha Salvage1, Abdul Waheed1, Chris Fry2, Rita Jabr1, 1University of Surrey, Guildford, UK, 2University of Bristol, Bristol, UK

119

An acute bout of exercise impacts cardiac CapZ regulation Glen Pyle1, 1Cardiovascular Research Group, Department of Biomedical Sciences, University of Guelph, Guelph, Ontario, Canada

120

Gender-dependent depression of myocardial contractility with activation of the estrogen receptor GPR30 Kaley Hogarth1, Glen Pyle1, 1Cardiovascular Research Group, Department of Biomedical Sciences,

University of Guelph, Guelph, Ontario, Canada

121

Prevascularized cardiac constructs to promote tissue survival and rapid host integration in vivo Meredith Roberts1, Dominic Tran1, Kareen Coulombe1,2, Nathan Palpant1, Maria Razumova1, Kaytlyn Gerbin1, Jill Weyers1, Sarah Dupras1, Michael Regnier1, Charles Murry1, Ying Zheng1, 1University of Washington, Seattle, WA, USA, 2Brown University, Providence, RI, USA

122

Modulation of Cardiac Stem Cell Growth by Oxygen Tension and Sirtuin 1 Michael A. Bellio1, Claudia O. Rodrigues1, Victoria Florea1, Aisha Khan1, Joshua M. Hare1, Ivonne H. Schulman1, 1University of Miami, Miami, Florida, USA

123

Covalent Modification of Cardiac Troponin C Alters Myocardial Ca2+ Sensitivity Ian Robertson1,2, Sandra Pineda-Sanabria1, Yin-Biao Sun2, Malcolm Irving2, Brian Sykes1, 1University of Alberta, Edmonton, Alberta, Canada, 2King's College London, London, UK

124

The Role of a Novel AMPK Activator in the Protection Against Angiotensin II-Induced Myocardial Hypertrophy Ian Robertson1, Nobutoshi Matsumura1, Shereen Hamza1, Carrie Soltys1, Miranda Sung1, Grant Masson1, Donna Beker1, Jason Dyck1, 1University of Alberta, Edmonton, Alberta, Canada

125

Cardiac evaluation in canine model of X-linked myotubular myopathy after correction of muscle pathology with AAV8-MTM1 Jennifer Strande1, Michael Lawlor1, David Mack2, Karine Poulard3, Melissa Goddard4, Jessica Snyder2, Robert Grange5, Jon Doering5, Virginie Latournerie6, Philippe Veron6, Hui Meng1, Lin Yang3, Fujun Liu3, Larine Buscara6, Samia Martin6, Michael O'Callaghan7, Federico Mingozzi6, Alan Beggs9, Anna Buj-Bello6, Martin Childers2, 1Medical College of Wisconsin, Milwaukee, WI, USA, 2University of Washington, Seattle, WA, USA, 3University of Florida, Gainesville, FL, USA, 4Wake Forest University, Winston Salem, NC, USA, 5Virginia Polytechnic and State University, Blacksburg, VA, USA, 6Généthon, Evry, France, 7Audentes Therapeutics, San Franscisco, CA, USA, 8University Pierre

and Marie Curie, Paris, France, 9Boston Children’s Hospital, Boston, MA, USA

126

A Novel Gene Therapy of Ribonucleotide Reductase for a Large Animal Heart Failure Model Shin Kadota1, Sam Teichman2, Michael Laflamme1, Charles Murry1, Michael Regnier1, Gregory Mahairas2, 1University of Washington, Seattle, WA, USA, 2BEAT Biotherapeutics Corp, Seattle, WA, USA

127

Cardioprotective role of miR-181c in obesity Brittany Dunkerly1,2, Junaid Afzal3, Igal Madar4, Djahida Bedja3, Jorge Henao-Mejia5, Richard Flavell5, Roopa Biswas6, Charles Steenbergen1, Samarjit Das1, 1Department of Pathology, Johns Hopkins University, Baltimore, MD, USA, 2Notre Dame of Maryland University, Baltimore, MD, USA,

3Department of Cardiology, Johns Hopkins University, Baltimore, MD, USA, 4Division of Nuclear Medicine, Russell H. Morgan Department of Radiology, Johns Hopkins University, Baltimore, MD, USA, 5Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA,

6Department of Anatomy, Physiology and Genetics, School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD, USA

128

Understanding the physiological role of gamma2-AMPK Naveen Bojjireddy1, Rong Tian1, 1University of Washington, Seattle, WA, USA

129

Pharmacological inhibition of soluble epoxide hydrolase preserves mitochondrial efficiency and cardiac function post-MI in aged mice Kristi L. Jamieson1, Victor Samokhvalov1, Maria Akhnokh1, Xiuhua Wang1, Zamaneh Kassiri1, John M. Seubert1, 1University of Alberta, Edmonton, Alberta, Canada

130

Organ-Specific VWF Promoter Activity in Response to Hypoxia and Microthrombotic Consequences Anahita Mojiri1, Maryam Nakhaei-Nejad1, Nadia Jahroudi1, 1University of Alberta, Edmonton, Alberta, Canada

131

Resveratrol improves exercise capacity in mice with heart failure by enhancing skeletal muscle oxidative capacity and vascular function Miranda Sung1, Nikole Byrne1, Ian Robertson1, Victor Samokhvalov1, Jody Levasseur1, Kelvin Jones1, John Seubert1, Jason Dyck1, 1Cardiovascular Research Centre, University of Alberta, Edmonton, AB, Canada

132

Sorcin interacts with the mitochondrial calcium uniporter and inhibits calcium transport in mitochondria Christian Silva-Platas1, Gerardo Garcia-Rivas1, 1Tecnologico de Monterrey, Monterrey, Nuevo Leon, Mexico

133

19,20-EDP protects HL-1 cardiac cells against LPS-induced cytotoxicity through activation of mitochondrial function and biogenesis. Victor Samokhvalov1, Kristi L. Jamieson1, John M. Seubert1, 1University of Alberta, Edmonton, Alberta, Canada

134

19,20-EDP targets mitochondrial pathways to produce protection of HL-1 cells against hypoxia-reoxygenation injury Victor Samokhvalov1, Kristi L. Jamieson1, John M. Seubert1, 1University of Alberta, Edmonton, Alberta, Canada

135

Identification of Pathologic Circulating Factors in Children with Dilated Cardiomyopathy Xuan Jiang1, Brian Reid1, Brian Stauffer1,3, Shelly Miyamoto2, Carmen Sucharov1, 1University of Colorado School of Medicine, Aurora, CO, USA, 2Children's Hospital Colorado, Aurora, CO, USA,

3Denver Health and Hospital Authority, Aurora, CO, USA

136

The mitochondrial calcium uniporter balances energetic supply with cardiac workload during sympathetic stress and modulates mitochondrial permeability transition in ischemia-reperfusion injury Timothy S. Luongo1, Jonathan P. Lambert1, Ancai Yuan1, Xueqian Zhang1, Santhanam Shanmughapriya1, Erhe Gao1, Polina Gross1, Steven R. Houser1, Muniswamy Madesh1, John W. Elrod1, 1Temple University, Philadelphia, PA, USA

137

Age-specific differences in ryanodine 2 receptor phosphorylation contribute to age-specific responses to phosphodiesterase 3 inhibition in heart failure therapy Kathleen Woulfe1, 1University of Colorado, Aurora, CO, USA

138

Selective blockade of β1 and β2 adrenergic receptors in a mouse model of pediatric hypertrophy Kathleen Woulfe1, 1University of Colorado, Aurora, CO, USA

139

The histone methyltransferase Smyd5 regulates cardiac hypertrophy Mickey R. Miller1, Caiyi C. Li2, Alexa Anderson1, Li Wang1, Stephen T. Smale2, Sarah Franklin1, 1Nora Eccles Harrison Cardiovascular Research & Training Institute, University of Utah, Salt Lake City, Utah, USA, 2Department of Microbiology, Immunology & Molecular Genetics, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA

140

Increased c-Myc protein levels are detrimental to cardiac function during pressure overload hypertrophy Margaret Bruce2, Dolena Ledee2, Lincoln Smith1, Danny El-Nachef1, Michael Portman1,2, Aaron Olson1,2, 1University of Washington, Seattle, WA, USA, 2Seattle Children's Research Institute,

Seattle, WA, USA

141

The effects of beta-adrenergic drugs on embryonic ventricular cell proliferation and differentiation and their impact on donor cell transplantation Tiam Feridooni1, Adam Hotchkiss1, Mark Baguma-Nibasheka1, Feixiong Zhang1, Kishore Pasumarthi0, 1Dalhousie University, Halifax, Nova Scotia, Canada

142

Revealing cardiac roles of the obscurin protein family Stephan Lange1, Matthew Klos1, Stephanie Myers1, Nancy Dalton1, Eric Devaney1, Kirk Peterson1, Ju Chen1, 1UC San Diego, La Jolla, CA, USA

143

Histone Deacetylase Inhibition Improves Cardiac Function and Attenuates Adverse Tissue Remodeling Post Myocardial Infarction with Upregulation of Wisp-1. Lillianne Harris1, Daniel Herr1, Harinath Kasiganesan1, Sabina Wang1, Donald Menick1,2, 1Medical University of South Carolina, Charleston, SC, USA, 2Ralph Johnson VA Medical Center, Charleston,

SC, USA

144

Biochemical and biophysical properties of the dilated cardiomyopathy associated cardiac troponin I mutation - P16T Jordan Klaiman1, Joseph Powers1, Robert Langan1, Maria Razumova1, Michael Regnier1, 1University of Washington, Seattle, WA, USA

145

Cardiac myocyte growth defect in lamin A/C deficient mice Kyohei Oyama1, W. Robb MacLellan1, 1University of Washington, Seattle, WA, USA

146

A high incidence of a MYBPC3 gene variant (25bp deletion) of South Asian descendents in the United States (DOSA study) Aravindakshan Jagadeesan1, Nalla Banu Durai2, Robert Molokie2, Suresh Govindan1, Stephanie Kliethermes1, Thriveni Sanagala1, Sakthivel Sadayappan1, 1Loyola University Chicago, Maywood, IL, USA, 2University of Illinois - Chicago, Chicago, IL, USA

147

β-adrenergic signaling activates glycogen autophagy in the heart Kimberley Mellor1, Ellie Stevens1, Upasna Varma2, Lea Delbridge2, 1University of Auckland, Auckland, New Zealand, 2University of Melbourne, Melbourne, Victoria, Australia

148

Developmental differences in neonate and adult cardiac extracellular matrix modulate cellular recellularization C.W Jenny Chang1, Janhavi Sharma1, Andrew Wong1, Jayne Squirrell2, Kevin Eliceiri2, Javier López1, 1University of California, Davis, Davis, USA, 2University of Wisconsin, Madison, Madison, USA

149

Acute AMPK activation attenuates cardiomyocyte glycogen accumulation induced by high glucose Upasna Varma1, Kimberley Mellor2, Lea Delbridge1, 1The University of Melbourne, Melbourne, Australia, 2The University of Auckland, Auckland, New Zealand

150

Association Between Serum Visfatin level And Coronary Artery Disease: A Meta-Analysis Pradyumna Agasthi1, Sivakanth Aloor2, Kalaivani Sivakumar1, Anekwe Onwuanyi1, 1Morehouse School of Medicine, Atlanta, GA, USA, 2University of Miami, Miller School of Medicine, Miami, FL, USA

151

Relationship between Serum YKL-40 levels and Coronary Artery Disease: A Meta-Analysis Pradyumna Agasthi1, Sivakanth Aloor2, Maihemuti Axiyan1, Anekwe Onwuanyi1, 1Morehouse School of Medicine, Atlanta, Georgia, USA, 2University of Miami, Miller School of Medicine, Miami, Florida, USA

152

Saturated high-fat diet-induced obesity increases adenylate cyclase of myocardial beta-adrenergic system and does not compromise cardiac function

Daniele Vileigas1, Adriana Deus1, Danielle Silva1, Loreta Tomasi1, Dijon Campos1, Katashi Okoshi1, Carlos Padovani1, Antonio Cicogna1, 1Sao Paulo State University, Botucatu, SP, Brazil

153

High unsaturated fatty acid diet does not rescue cardiac dysfunction or lipid energy metabolism in rats with aortic stenosis Loreta C. Tomasi1,4, Dijon H. S. Campos1, Danielle F. Vileigas1, Katashi Okoshi1, Carlos H. Padovani2, Gilson M. Murata3, Stephen C. Kolwicz4, Antonio C. Cicogna1, 1Department of Internal Medicine, Botucatu School of Medicine - Sao Paulo State University, Botucatu, SP, Brazil, 2Department of Biostatistics, Botucatu Institute of Biosciences - Sao Paulo State University, Botucatu, SP, Brazil, 3Department of Biochemistry, Institute of Chemistry - University of Sao Paulo, Sao Paulo, SP, Brazil,

4Mitochondria & Metabolism Center, Department of Anesthesiology and Pain Medicine, University of Washington, Seattle, WA, USA

154

Determining the Role of Branched Chain Amino Acid Utilization in Cardiac Substrate Utilization Lauren Abell1, Stephen Kolwicz1, Tao Li1, Daniel Raftery1, Rong Tian1, 1University of Washington, Seattle, USA

155

Right ventricular energy metabolism in a porcine model of acute pressure overload Masaki Kajimoto1, Muhammad Nuri1,2, Dolena Ledee1, Aaron Olson1,2, Nancy Isern3, Michael Portman1,2, 1Seattle Children’s Research Institute, Seattle, WA, USA, 2University of Washington, Seattle, WA, USA, 3Pacific Northwest National Laboratories, Richland, WA, USA

156

Filling the HFpEF Gap to Guide New Therapies - The Development of HFpEF Animal Models Kersten Small1, Huawei Zhao1, Chin-Hu Huang1, Shu-Yu Sun1, Valdeci DaCunha1, Jeffrey Madwed1, 1Merck Research Labs, Kenilworth, NJ, USA

Author Index Aba-Omer, Omer 085

Abdellatif, Maha 049

Abdullah, Salwa 098

Abell, Lauren 154

Adly, George 009

Afzal, Junaid 127

Agasthi, Pradyumna 150, 151

Akhnokh, Maria 129

Alegre-Cebollada, Jorge 083

A. Linke, Wolfgang 083

Aloor, Sivakanth 150, 151

Alvarez, Roberto 019

Anderson, Alexa 139

Anderson, Todd 001

Annapoorani, Ponnambalam 021

Aponte, Angel 005, 006, 048

Appachi, Swathi 013

Atieno, Nancy 038

August, Ben 068

Avolio, Ennio 041

Axiyan, Maihemuti 151

Ayaz, Omar 086

Baan, Júlia A 040

Baguma-Nibasheka, Mark 141

Baines, Christopher 007, 062

Baker, Anthony J 061

Bammler, Theo 044

Bandyopadhyay, Gautam 041

Banyasz, Tamas 071, 077

Baranyai, Tamás 040

Barefield, David 068

Basisty, Nathan 112

Bechara, Luiz 034

Bedja, Djahida 028, 127

Beggs, Alan 125

Beker, Donna 124

Belardinelli, Luiz 077

Bellio, Michael A 122

Bernal-Ramírez, Judith 065

Bernstein, Irwin 012

Bers, Donald 004, 014, 015, 047, 070, 078

Berthiaume, Jessica 043

Bezold, Kristina 087

Bisetto, Sara 084

Biswas, Nilima 041

Biswas, Roopa 127

Bjorndahl, Trent 001

Blanco, Elvin 065

Bojjireddy, Naveen 128

Boroumandi, Arash 069

Bossuyt, Julie 078

Bouatra, Souhaila 001

Boylston, Jennifer 051

Breitkreuz, Martin 083

Bruce, Jim 046

Bruce, Margaret 140

Brum, Patricia 034

Buj-Bello, Anna 125

Burelle, Yan 039

Buscara, Larine 125

Byrne, Nikole 131

Cai, Wenfeng 009, 010

Campos, Dijon 152

Campos, Juliane 034

Cao, Li 026, 055, 056

Cao, Quan 093

Cass, Ashley 030

Chang, CW Jenny 148

Chan, Trevor 061

Chavez, Juan 046

Chen, Che-Hong 057

Cheng, Hongqiang 041

Cheng, Yuanhua 097

Chen, Haodong 023

Chen, Iris 030

Chen-Izu, Ye 071, 077, 078

Chen, Ju 142

Chenliu, Cici 108

Chen, Xiyao 074, 075

Chen, Yi 008

Chen, Yong 051

Chiamvimonvat, Nipavan 078

Chiao, Ying Ann 112

Chien, Wei Ming 089

Childers, Martin 125

Chin, Michael 003, 044, 089

Chirasani, Venkat R 041

Choi, Sung Won 103

Choi, Yongseon 046, 060, 067

Cicogna, Antonio 152, 153

Coulombe, Kareen 121

Cowan, Conrad L 016

Craig, Roger 068

Csont, Tamás 040

DaCunha, Valdeci 156

Dalton, Nancy 142

Dan, Pauline 108

Dash, Rajesh 061

Das, Samarjit 028, 127

Davidson, Christopher 038

Davis, Gregory 059

de Lange, Willem 018, 025, 102

Delbridge, Lea 147, 149

Despa, Florin 020, 027

Despa, Sanda 020, 027

Deus, Adriana 152

Devaney, Eric 142

Devarajan, Asokan 038

Dholakia, Jhanvi 063

Dinca, Ana 089

Dincer, T Umut 094

Disatnik, Marie-Helene 057

Djukovic, Danijel 103

Doan, Jessica 008

Doering, Jon 125

Domingues, Lais 053

Dong, Maolong 064

Dong, Zhen 082

Dorn, Gerald 009, 039

Dourado, Paulo 034

Dudley, Samuel C 002

Dunkerly, Brittany 028, 127

Dupras, Sarah 121

Durai, Nalla Banu 146

Dyck, Jason 001, 124, 131

Edwards, Andrew 015

Eldstrom, Jodene 036

Elgudin, Yakov 022

Eliceiri, Kevin 148

El-Nachef, Danny 140

Elrod, John W 136

El-Sayed, Farid 019, 052

Emancipator, Steven 022

Emathinger, Jacqueline 014

Emes, Robert 036

Evangelista, Alicia 006

Ezekowitz, Justin 001

Fan, Guo-Chang 009

Farrell, Emily 102

Farris, Stephen 032, 113

Feaster, Tromondae K 031

Fedida, David 036

Ferdinandy, Péter 040

Feridooni, Hirad 069, 095

Feridooni, Tiam 141

Ferreira, Julio 034, 035, 053, 057

Finkel, Toren 048

Fischer, Karin 079

Fisher, Kristina 072

Fitzsimons, Daniel 025

Flavell, Richard 028, 127

Florea, Victoria 122

Franklin, Sarah 023, 139

Fridolfsson, Heidi N 041

Fry, Chris 118

F. Vileigas, Danielle 153

Gao, Chen 030, 050

Gao, Erhe 136

García-García, Alejandra 065

Garcia-Menendez, Lorena 046, 060

García, Noemí 065

Garcia-Patino, Marysol 109, 110

García-Rivas, Gerardo 029, 037, 065, 132

Gardner, George 010

Garza López, Jesús Roberto 029, 065

Genge, Christine 105

Gerbin, Kaytlyn 088, 121

Gerdes, Martin 043

Ginsburg, Kenneth 078

Goddard, Melissa 125

Goldhaber, Joshua I 099

Gomes, Kátia 034

Gong, Jianli 050

Goodson, Jamie 044

Govindan, Suresh 068, 146

Grandi, Eleonora 015

Grange, Robert 125

Grimes, Adrian 102

Gross, Polina 136

Guatimosim, Silvia 034

Gucek, Marjan 005, 006, 051

Gude, Natalie 019, 072

Guerrero-Beltrán, Carlos Enrique 065

Gu, Haiwei 103

Gulick, James 059

Guo, Wei 058

Gutierrez-Aguilar, Manuel 062

Gutierrez Cortes, Nicolas 100

Hadland, Brandon 012

Hamdani, Nazha 083

Hamza, Shereen 124

Han, Beomsoo 001

Hare, Joshua M 122

Hariharan, Nirmala 014, 019

Harrington, Josephine 054

Harris, Lillianne 143

Harris, Samantha 087

Hatch, Fiona 118

Headrick, John P 041

He, Guanglong 026, 055, 056

Hegyi, Bence 071, 077, 078

Helterline, Deri 032, 113

He, Minzhen 049, 066

Henao-Mejia5, Jorge 028

Henao-Mejia, Jorge 127

Herr, Daniel 143

Hill, Joseph 117

Hofsteen, Peter 073

Hogarth, Kaley 120

Hogarth, Kaley A 097

Holmstrom, Kira 048

Hong, Charles C 031

Hong, Jennifer 038

Horvath, Balazs 071

Hotchkiss, Adam 141

Houser, Steven R 136

Howlett, Susan 069, 086, 095, 114, 115

H. Padovani, Carlos 153

H. S. Campos, Dijon 153

Huang, Chin-Hu 156

Huang, Wenrui 080

Hua, Rui 116

Hwang, Hyun Seok 031

Irving, Malcolm 123

Isern, Nancy 155

Izu, Leighton T 071, 077, 078

Jabr, Rita 118

Jagadeesan, Aravindakshan 146

Jahroudi, Nadia 130

Jamieson, Kristi L 129, 133, 134

Jannig, Paulo 034

Jansen, Hailey 115

Jiang, Min 009, 024

Jiang, Xuan 135

Jian, Zhong 071, 077, 078

Jia, Zhengping 110

J. Lusis, Aldons 023

Jones, Christina 012

Jones, Daniel 012

Jones, Kelvin 131

Jones, Peter 063

Kadota, Shin 126

KAJIMOTO, HIDEMI 096

Kajimoto, Masaki 155

Kamp, Timothy J 031

Kanashiro-Takeuchi, Rosemeire 080

Kang, Jeanney 099

Kapiloff, Michael S 017

Karagodin, Ilya 085

Karbassi, Elaheh 023

Kar, Sumit 018, 025

Kasiganesan, Harinath 143

Kassan, Adam 041

Kass, David 028

Kassiri, Zamaneh 129

Katus, Hugo A 090

Katzenellenbogen, Benita 013

Katzenellenbogen, John 013

Kazmierczak, Katarzyna 080

Kellermayer, Dalma 042

Kent, Oliver 028

Khan, Aisha 122

Kim, Maengjo 060

Klaiman, Jordan 144

Kliethermes, Stephanie 146

Klos, Matthew 142

Knollmann, Björn C 031

Koch, Sheryl 024

Kohr, Mark 028, 091

Kolwicz, Stephen 067, 076, 101, 112, 153, 154

Kowaltowski, Alicia 034

Kranias, Evangelia 009, 010, 024

Krenz, Maike 007

Kryshtal, Dmytro O 031

Kurdistani, Siavash 023

Kuster, Diederik 068

Laflamme, Michael 126

Lamba, Harveen 022

Lambert, Jonathan P 136

Lambert, Rebekah 020

Lam, Chi Keung 009, 010

Lam, Kit S 078

Lam, Maggie PY 093, 094

Langan, Robert 144

Lange, Stephan 142

Lara-Pezzi, Enrique 040

Latournerie, Virginie 125

Lau, Edward 093, 094

Lawlor, Michael 125

Ledee, Dolena 140, 155

Lee, Chi Fung 046

Lee, Kyounghwan 068

Legare, Jean Francois 116

Leichert, Lars 083

Leonard, Andrea 079

Leszek, Przemyslaw 040

Leung, Anthony 028

Levasseur, Jody 131

Li, Alison 106

Liang, Bo 104

Liang, Jingsheng 080

Liao, Zhandi 070

Li, Caiyi C 139

Li, Guangping 030

Li, Jie 092

Li, Jinling 017

Li, Lei 008

Lima, Vanessa 035

Lin, Eric 105, 108

Lin, Yi 003

Lin, Yongshun 006

Li, Tao 067, 076, 154

Liu, Fujun 125

Liu, Guansheng 009, 010, 024

Liu, Hong 002

Liu, Man 002

Liu, Miao 027

Liu, Xiangwei 082

Liu, Yonggang 003, 044

Li, Yueh Alison 104

López, Javier 148

Lozano, Omar 065

Luo, Jun 032

Luongo, Timothy S 136

Lusis, Aldons 011

Lu, Xiyuan 047

MacCoss, Michael 112

MacDonald, James 044

MacDonald, Jennifer 095

Mack, David 125

MacLellan, W Robb 003, 096, 145

MacLeod, Kathleen 109, 110

MacLeod, Sarah 116

Madar, Igal 127

Madesh, Muniswamy 136

Madwed, Jeffrey 156

Mahairas, Gregory 126

Mahata, Sumana 041

Mahata, Sushil K 041

Mandal, Rupasri 001

Marbán-González, Alberto 037

Marcinek, David 111

Margulies, Kenneth 020, 027

Marshall, Kurt 007

Martinez, Eliana C 017

Martin, Samia 125

Martinson, Amy 003

Masson, Grant 124

Matsumura, Nobutoshi 124

Mattos, Ana 067

Mátyás, Csaba 042

McConnell, Mark 098

McCulloch, Andrew 015

McLay, Janet 063

McLendon, Patrick 059

Mehri, Avin 003

Mellor, Kimberley 147, 149

Menazza, Sara 005, 013, 048, 054

Mendler, Luca 040

Meng, Hui 125

Menick, Donald 143

Merkely, Béla 042

M. Fernandez, Julio 083

Miller, Cory 038

Miller, Mickey R 139

Minamisawa, Susumu 045

Mingozzi, Federico 125

Miyamoto, Shelly 135

M. Murata, Gilson 153

Mochly-Rosen, Daria 034, 057

Moghtadaei, Motahareh 114, 116

Mojiri, Anahita 130

Molkentin, Jeffery 008, 059

Molokie, Robert 146

Monsanto, Megan 072

Monte, Emma 023

Montgomery, Megan D 061

Moon, Randall 012, 073

Morotti, Stefano 015

Morris, Andrew 027

Moshref, Maryam 019

Moss, Richard 025

Most, Patrick 090

Moussavi-Harami, Farid 111

Murphy, Elizabeth 005, 006, 013, 048, 051, 054,

091

Murray, Christopher 036

Murray, Jason 008, 111

Murry, Charles 003, 012, 073, 079, 088, 121, 126

M. Vondriska, Thomas 023

Myagmar, Bat-Erdene 061

Myers, Stephanie 142

Myles, Rachel 004

Nabben, Miranda 067

Nair, Sreejayan 026, 055, 056

Nakhaei-Nejad, Maryam 130

Namakkal-Soorappan, Rajasekaran 068

Nanasi, Peter P 071

Narasimhan, Madhusudhanan 038

Németh, Balázs Tamás 042

Ng, Dominic 093

Nguyen, Nathalie 014

Nicolaou, Persoulla 009

Nogueira Varela, Julia 109, 110

Nuri, Muhammad 155

Nyamandi, Vongai 109, 110

O'Blenes, Stacy 116

O'Callaghan, Michael 125

Okoshi, Katashi 152, 153

Oláh, Attila 042

Olson, Aaron 140, 155

Onusko, Evan 024

Onwuanyi, Anekwe 150, 151

Ornelas, Nancy 065

Oropeza-Almazán, Yuriana 037

O'Sullivan, M Lynne 097

Oudit, Gavin 001

Oyama, Kyohei 096, 145

Pabon, Lil 012, 073, 079

Pacher, Pál 040

Padovani, Carlos 152

Palmiter, Richard 060

Palpant, Nathan 012, 073, 121

Parks, Randi 048

Parry, Traci 117

Pasqua, Teresa 041

Passariello, Catherine L 017

Pasumarthi, Kishore 141

Patel, Hemal H 041

Patel, Sajni 006

Peng, Xiaoli 020, 027

Peterson, Kirk 142

Pfleger, Jessica 049

Philipson, Kenneth D 099

Pineda-Sanabria, Sandra 123

Ping, Peipei 093, 094

Pipicz, Márton 040

Polidovitch, Nazari 069

Polina, Iuliia 116

Portman, Michael 043, 140, 155

Poulard, Karine 125

Powers, Joseph 144

Pyle, W Glen 095, 097, 119, 120

Qin, Xing 026, 055, 056

Queliconi, Bruno 034

Quijada, Pearl 014, 019

Quintana, Megan 117

Qu, Yang 108

Rabinovitch, Peter 112

Radovits, Tamás 042

Rafferty, Sara 114, 115

Raftery, Daniel 103, 154

Rajamani, Sridharan 077

Rajasekaran, Namakkal S 038

Ralphe, J Carter 018, 025, 102

Ramasamy, Sakthivel 038

Rau, Christoph 011, 023

Rayani, Kaveh 104, 106, 107

Razumova, Maria 121, 144

Regnier, Michael 097, 111, 121, 126, 144

Reid, Brian 135

Reinecke, Hans 079

Ren, Jun 026, 055, 056, 058, 064, 074, 075, 082

Ren, Shuxun 011, 030

Ren, Vincent 045

Resende, Cibele 034

Reyes-Alvarez, Eduardo 037

R. Hoidal, John 038

Ripplinger, Crystal 004

Ritterhoff, Julia 090

Robbins, Jeffrey 059

Roberts, Meredith 012, 121

Robertson, Ian 123, 124, 131

Robitaille, Aaron 073

Rodrigues, Claudia O 122

Rodriguez, Marita 079

Roe, Nathan 033

Rojas, Ana I 080

Romay, Milagros 011

Rosa-Garrido, Manuel 023

Rose, Robert 069, 086, 114, 115, 116

Roth, David M 041

Rubinstein, Jack 009, 024

Ruppert, Mihály 042

Ruzzo, Walter 012

Ryba, David M 016

Sack, Michael 051

Sadayappan, Sakthivel 068, 146

Salunga, Hazel 014, 019

Salvage, Samantha 118

Samokhvalov, Victor 129, 131, 133, 134

Sanagala, Thriveni 146

Sanatani, Shubhayan 108

Sánchez-Freire, Verónica 031

Sapp, John 116

Sayed, Danish 066

Schilling, Jan M 041

Schulman, Ivonne H 122

Seitz, Andreas 090

Senapati, Sanjib 041

Sen, Suvajit 041

Seubert, John M 129, 131, 133, 134

Shafaattalab, Sanam 105

Shanmughapriya, Santhanam 136

Shao, Dan 067, 103

Shao, Qin 091

Sharma, Janhavi 148

Shaul, Philip 013

Shaw, John 078

Shay-Winkler, Kritton 059

Sheng, Xiao Ye 108

Sheu, Shey-Shing 084

Shi, Guangbin 002

Shimkunas, Rafael 071, 077, 078

Silva, Danielle 152

Silva, Ivson 035

Silva-Platas, Christian 132

Simpson, Paul C 061

Sivaguru, Mayandi 068

Sivakumar, Kalaivani 150

Smale, Stephen T 139

Small, Kersten 156

Smelter, Dan 018, 025

Smith, Eric 067, 100

Smith, Lincoln 140

Sniadecki, Nathan 079

Snyder, Jessica 125

Solaro, R John 016

Soltys, Carrie 124

Song, Moshi 039

Sparapani, Rodney 085

Squirrell, Jayne 148

Sreejayan, Nair 075

Srodulski, Sarah 020

Stauffer, Brian 135

Steenbergen, Charles 005, 028, 091, 127

Stefani, Enrico 023

Steinberg, Susan 050

Stempien-Otero, April 032, 113

Stevens, Charles 106, 107

Stevens, Ellie 147

Stoehr, Andrea 006

Strande, Jennifer 085, 125

Sucharov, Carmen 135

Su, Huabo 092

Sun, Aijun 082

Sung, Miranda 001, 124, 131

Sun, Haipeng 076

Sun, Junhui 005, 013, 051

Sun, Shu-Yu 156

Sun, Xiaolei 082

Sun, Yin-Biao 123

Sussman, Mark 014, 019, 052, 072

Swigart, Philip M 061

Sykes, Brian 123

Szabó, Gábor 042

Szabó, Lilla 042

Szczesna-Cordary, Danuta 080

Tang, Wei 043

Tardiff, Jil 098

Teichman, Sam 126

Tian, Rong 033, 046, 060, 067, 076, 103, 112, 128,

154

Tibbits, Glen 104, 105, 106, 107, 108

Tomasi, Loreta 101, 152, 153

Török, Marianna 042

Torrente, Angelo G 099

Tran, Dominic 121

Tsai, Jesse 044

Tsushima, Robert 069

Ueta, Cintia 035, 053, 057

Vafiadaki, Elizabeth 009

van Dijk, Sabine 087

Van Eyk, Jennifer 094

Varga, Zoltán 040

Varma, Upasna 147, 149

Venkatraman, Vidya 094

Verma, Nirmal 027

Veron, Philippe 125

Vileigas, Daniele 152

Waddell, Helen 063

Wadosky, Kristene 043

Waheed, Abdul 118

Wang, Ding 093

Wang, He 030

Wang, Jessica 011, 023

Wang, Li 139

Wang, Lianguo 004

Wang, Pei 081, 100

Wang, Peng 082

Wang, Sabina 143

Wang, Shuyi 064

Wang, Wang 067, 081, 084, 100

Wang, William 050

Wang, Xiaoming 074, 075

Wang, Xinghua 030

Wang, Xiuhua 129

Wang, Xuejun 092

Wang, Yibin 011, 030, 045, 050, 076

Wang, Zhihua 030

Webster, Nicholas J G 041

Wei, Ning 092

Weiss, James 023

Weldy, Chad 044

Westhoff, Maatje 036

Weyers, Jill 121

Whitehead, Kevin 038

White, Kevin 072

Wieczorek, David F 016

Willis, Monte 043, 117

Wishart, David 001

Wnek, Gary 022

Wolska, Beata M 016

Wong, Andrew 148

Woulfe, Kathleen 137, 138

Wu, Ella 063

Wu, Joseph C 031

Wu, Yong 023

Xiao, Xinshu 030

Xu, Shangcheng 081, 100

Yang, Lin 125

Yang, Xiulan 079

Yei, Kevin 041

Yin, Zhiyong 058

Yokota, Tomohiro 030, 045

Yuan, Ancai 136

Yuan, Chen-Ching 080

Zaini, Audrey 099

Zambelli, Vanessa 034

Zemljic-Harpf, Alice 041

Zhang, Feixiong 141

Zhang, Hanming 092

Zhang, Huiliang 084, 100

Zhang, Jianhua 031

Zhang, Joe 063

Zhang, Rui 099

Zhang, Xueqian 136

Zhang, Yiqiang 003

Zhang, Zhen 076, 103

Zhao, Huawei 156

Zhao, Wen 009, 010

Zheng, Tiantian 026, 055, 056

Zheng, Ying 012, 121

Zhu, Chaoqun 058

Zhu, Hong 082

Zhu, Xiaoling 074

Zordoky, Beshay 001