Acore-CMS 2011.12.5

Activities of HAB group in Acore-CMS program

Mitsunori Iwataki Faculty of Science, Yamagata University, Japan

Introduction HAB: Harmful Algal Blooms

- Harmful red tide forming algal species which causes fish and shellfish mass mortalities.

- Toxic algal species of which produced toxic substances accumulated in shellfishes (or fishes).

Research of HAB group is therefore including various fields, e.g., taxonomy, ecophysiology, toxicology, oceanography, distribution of species, dinoflagellate cysts, etc.

In Acore-CMS, we provide the biodiversity of phytoplankton including HAB species, and many aspects of such HAB information .

National Group Leaders

Indonesia (4): Hikmah THOHA (RCO, LIPI) Japan (6): Mitsunori IWATAKI (GL) (Yamagata University) Malaysia (5): LIM Po Teen (Universiti Malaysia Sarawak (UNIMAS)) Philippines (2): Elsa FURIO (NFRDI) Thailand (5): Thaithaworn LIRDWITAYAPRASIT (Chulalongkorn University) Vietnam (10): NGUYEN Van Nguyen (Res. Inst. Mar. Fish. (RIMF))

HAB Members (2011)

National Group Leaders Indonesia (4→4): Hikmah Thoha (LIPI) Japan (6): Mitsunori Iwataki (Yamagata University), GL Malaysia (5→9): Lim Po Teen (Universiti Malaysia Sarawak) Philippines (2→6): Elsa Furio (NFRDI) Thailand (5→6): Thaithaworn Lirdwitayaprasit (Chulalongkorn University) Vietnam (10→13): Nguyen Van Nguyen (Res. Inst. Mar. Fish. (RIMF))

New members in YS2012 Indonesia (1): Arief Rachmann Philippines (5): Rhodora Azanza, Juan Relox Jr., Ulysses M. Montojo, Norvida C. Gatdula, Aleta Yñiguez Malaysia (6): Lim Hong Chang, Leaw Chui Pin, Sazlina Salleh, Yong Ai Hua, Rhoziawati Hohd Razali, Siti Aishah Abdullah Thailand (1): Penjai Sonpongchaiyakul Vietnam (8): Pham The Thu, Pham Xuan Ky, Nguyen Phuong Anh, Dand Quoc Minh, Phan Bao Vi, Le Thanh Thug, Luu Xuan Hoa, Luong Quang Doc

HAB Members (2012)

Planed Travels (2011)

Visitor: M. Iwataki and T. Omura (Indonesia) (Pattern 1) Date: Sept 2011 (1 week) Counterpart: Hikmah THOHA（RCO, LIPI） Purpose: Marine Phytoplankton Database, Research for HAB species

Visitor: K. Furuya (Thailand) (Pattern 1) Date: Aug 2011 (1 week) Counterpart: Thaithaworn LIRDWITAYAPRASIT（Chulalongkorn Univ） 　　　　Researcher in Department of Marine and Coastal Resources Purpose: Eutrophication database

Visitor: Valeriano BORJA (Japan) (Pattern 2) Date: Jan or Feb 2011 (1 week) Counterpart: M. Iwataki, T. Omura Purpose: Marine Phytoplankton Database

Other budget sources Japan: M. Iwataki, T. Omura and Y. Fukuyo - Grants-in Aid for Scientific Research (Kakenhi, Overseas, by JSPS) Submitted a research (FY2012-2015). <20,000,000JPY for 4 years (amounts in application)

- Bilateral Programs (International Collaborations, by JSPS) Submitted with Hikmah Thoha (Indonesia-LIPI) (FY2012-FY2014) ca. 7,400,000JPY for 3 years (amounts in application)

Y. Fukuyo, T. Omura and M. Iwataki - Grants-in Aid for Scientific Research (Publication support) Submitted for publication support of “Marine Phytoplankton Database”

- Grants-in Aid for Scientific Research (Kakenhi, Overseas, by JSPS) Submitted a collaborative study (FY2012-2014) ca. 47,000,000JPY for 3 years (amounts in application)

Other budget sources Indonesia: RCO-LIPI, RCO-LIPI-JSPS (submitted JSPS bilateral)

Malaysia: Malaysian Government – MoHE (RM83,000), MOSTI & MoHE (RM49,000, RM169,000), International Foundation of Science, Sweden (USD10,000) (-2011)

Philippines: locally funded by NFRDI and BFAR, DOST (PhilHAB, -2015)

Thailand: NRCT-JSPS (500,000THB, -2011), ASP research (120,000THB)

Vietnam: RIMF (small..)

For training course: GEOHAB-Asia by Dr. Gires Usup (UKM), WESTPAC-HAB by Dr. Yasuwo Fukuyo (Univ. Tokyo)

Research Plan in Acore-CMS (2011-) Our HAB group is now in Biodiversity group (G2H),

Difficulties: HAB study is a sort of applied biology including taxonomy, ecophysiology, toxicology, distribution, dinocysts, development of toxin detection tools…

Dr. Nishida: hoped HAB group to join Biodiversity to cover various kind of organism, and we can do HAB study under Acore-CMS.

In Southeast Asia, (1) Marine Phytoplankton Database (2) Eutrophication Database

Both will be established based on using information already obtained.

Research in HAB group (1) Marine Phytoplankton Database - collection and compiling microalgal photos (2011) - publication of Atlas of Microalgae (2012) (including HAB species, dinoflagellates, diatoms etc.) - occurrence and distribution of HAB species, additional species data, (2013-)

(2) Eutrophication Database - collection of nutrients information (2011, Gulf of Thailand) - collection of nutrients information (2012-, Manila Bay, Jakarta Bay)

(3) Regional Research - Local and collaborative study on HAB species impacts

(4) Collaborative Study - with G1 Physics: Mass mortality of shellfish in Bangpakong River Estuary

(5) “Integrated Ecosystem Research” in Acore-CMS

Marine Phytoplankton Database

Now preparing publication of “Marine Phytoplankton”, edited by, Iwataki, Omura, Takayama, Borja, Fukuyo

*compiling photos already taken (LM, FM, SEM) *hope to join all members in HAB group

Contents Species List (M. Iwataki) HAB species (Val Borja) Dinoflagellates (M. Iwataki, H. Takayama, Y. Fukuyo) Other flagellates (M. Iwataki) Diatoms (T. Omura)

Marine Phytoplankton Database

Eutrophication Database

Collection and compiling the information of nutrients, chlorophyll, and biomass of phytoplankton around megacity such as Gulf of Thailand, Manila Bay and Jakarta Bay.

Finally we can compare with Marine Phytoplankton Database e.g., distribution characteristics of HAB species in each eutrophicated coastal area.

-  promoted by Dr. Ken Furuya -  Gulf of Thailand (2011) -  Manila Bay, Jakarta Bay (2012-)

Regional HAB Research Indonesia: (2011) - Photos provided (2011-2015) - Phytoplankton research in Banggai Is., Lamalara Is, Jakarta Bay, etc.

Malaysia: (2011) - Photos provided - Species diversity of Pseudo-nitzschia in Malaysia - A review on Ecology, Biology and Bloom dynamics of Pyrodinium bahamense - Toxigenesis of PST-producing dinoflagellates (Complete sequence of sxtI) - Benthic harmful dinoflagellates in Malaysia - Yessotoxin producing dinoflagellates in Malaysia - Harmful Algal Blooms in Sabah (2012-) - Species Inventory of HABs species in Malaysia (Molecular, Ballast, FISH..) - Bloom dynamics of selected HABs species - Training course on selected HABs species

Regional HAB Research Philippines: (2011) -  Photos provided (2011-2015) -  Temporal and spatial variations of dinocyst in Manila Bay (NFRDI and BFAR) -  Characteristics and variations of ciguatoxins accumulation (NFRDI) - Philippine HAB program (UPMSI, 2011-2015) Thailand: (2011-) - Red tide monitoring by DMCR (2012-) -  Effects of massive flood discharge on coastal ecosystems in the Upper Gulf of Thailand: a case study on eutrophication and red tide phenomena Vietnam: (2011) - Photos provided (2012-) - Determining the causative species for domoic acid accumulation in bivalves. - Diversity and classification of phytoplankton in Vietnam waters. - Identification of HAB species using FISH method.

Collaborative Study

- with G1 Physics: Mass mortalities of shellfishes in Bangpakong River Estuary, Upper Gulf of Thailand have occurred in the late September to the early October every year. However, the mechanism of the mass mortality was still unclear.

G1 Physics group measures the time series data of dissolved oxygen, temperature, salinity, chl a, and water current.

Collaboration was proposed by Dr. Morimoto (GL, Physics). We can support from the aspect of phytoplankton composition and the presence of HAB species (Heterocapsa circularisquama?).

“Integrated Ecosystem Research”

“Integrated Ecosystem Research”

HAB group can join any field,

Difficulty: species composition of phytoplankton is easily changeable and difficult to understand the typical species composition of the area only by several sampling.

-  Species composition of microalgae (including planktonic and benthic species) -  Presence of Harmful Algal Bloom (HAB) responsible species -  Epiphytic HAB species composition on seagrass