Haematology and Blood Transfusion 28----------------------------------Hamatologie und Bluttransfusion

Edited by H. Heimpel, VIm· D. Huhn, Munchen C. Mueller-Eckhardt, GieSen G. Ruhenstroth-Bauer, Munchen

Modern Trends in Human Leukemia V New Results in Clinical and Biological Research Including Pediatric Oncology

Organized on behalf of the Deutsche Gesellschaft flir Hamatologie und Onkologie. Wilsede, June 21-23, 1982

Wilsede Joint Meeting on Pediatric Oncology II Hamburg, June 25126, 1982

Edited by R. N eth, R. C. Gallo, M. F. Greaves, M. A. S. Moore, and K. Winkler

With 192 Figures and 151 Tables

Springer-Verlag Berlin Heidelberg New York Tokyo 1983

Dr. RolfNeth, Universitaets-Krankenhaus Eppendorf, Medizinische Klinik, Abteilung fuer klinische Chemie, MartinistraBe 52, 2000 Hamburg 20, Federal Republic of Germany Dr. Robert C. Gallo, National Cancer Institute, Laboratory of Tumor Cell Biology, Bethesda, MD 20014, USA Dr. Melvyn F. Greaves, Imperial Cancer Research Fund Laboratories, P.O. Box 123, London WC2 A3PK, Great Britain Dr. Malcolm A. S. Moore, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA Dr. Kurt Winkler, Universitaets-Kinderklinik, Abteilung fuer Haematologie und Onkologie, MartinistraBe 52, 2000 Hamburg 20, Federal Republic of Germany

Supplement to BLUT - Journal Experimental and Clinical Hematology Organ of the Deutsche Gesellschaft fUr Hiimatologie und Onkologie der Deutschen Gesellschaft fUr Bluttransfusion und Immunhiimatologie and of the Osterreichische GesellschaftfUr Hiimatologie und Onkologie

ISBN -13: 978-3-540-11858-9 e-ISBN -13: 978-3-642-68761-7 DOl: 10.1007/978-3-642-68761-7

Library of Congress Cataloging in Publication Data. Main entry under title: Modern trends in human leukemia V. (Haematology and blood transfusion = Hlimatologie und Bluttransfusion; 78) "Orga­nized on behalf of the Deutsche Gesellschaft flir Hlimatologie und Onkologie and the Deutsches Krebsforschungszentrum, Wilsede, June 1982." "Supplement to Blut." Bibliography: p. Includes index. I. Leukemia-Congresses. 2. Leukemia in children-Congresses. I. Neth, Rolf. II. Deut­sche Gesellschaft flir Hlimatologie und Onkologie. III. Deutsches Krebsforschungszen­trum Heidelberg. IV. Series: Haematology and blood transfusion; 78. [DNLM: I. Leu­kemia-Congresses. WI HAI655 v. 281 WH 250 M689 1982) RC643.M62 1983 616.99'419 83-505 ISBN -13: 978-3-540-11858-9 (U.S.)

This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned specifically those of translation, reprinting, re-use ofillustra­tions, broadcasting, reproduction by photocopying machine or similar means, and stor­age in data banks. Under § 54 of the German Copyright Law where copies are made for other than private use a fee is payable to "Verwertungsgesellschaft Wort", Munich.

© Springer-Verlag Berlin Heidelberg 1983

The use of registered names, trademarks etc. in this publication does not imply even in the absence of a specific statement, that such names are exempt from the relevant pro­tective laws and regulations and therefore free for general use.

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2123/3321/543210

Scientific Organisation of the Sessions

New Strategies in Leukemia Diagnostic and Therapy

Heimpel, Hermann, Zentrum fuer Innere Medizin und Kinderheil­kunde der Universitaet Ulm, Steinhoevelstrasse 9, 7900 Ulm, Fed­eral Republic of Germany Hoflbrand, A. Viktor, Dept. of Haematology, Royal Free Hospital, Rond Street, Hampstead, London NW32QG, Great Britain

Mechanism of Malignant Transformation Virology

Gallo, Robert, National Cancer Institute, Bldg. 37, Room 6B04, Bethesda, MA 20014, USA Hausen, Harald zur, Institut fuer Virologie im Zentrum fuer Hygie­ne, Hermann-Herder-Strasse 11, 7800 Freiburg, Federal Republic of Germany

Cellbiology

Greaves, Melvyn F., Imperial Cancer Research Fund Laboratories, P.O. Box 123, London WC2A3PX, Great Britain Moore, Malcolm A.S., Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA

Immnnological Aspects in Malignancy

Cooper, Max, University of Alabama, School of Medicine, Compre­hensive Cancer Center, Birmingham, AL 35294, USA Mitchison, N. Avrion, University College London, Tumour Immu­nology Unit, Gower Street, London WCIE6BT, Great Britain

Wilsede Joint Meeting on Pediatric Oncology II

Kabisch, Hartmut, Universitaetskinderklinik, Abteilung flir Haema­tologie und Onkologie, Martinistrasse 52, 2000 Hamburg 20, Federal Republic of Germany Winkler, Kurt, Universitaetskinderklinik, Abteilung flir Haemato­logie und Onkologie, Martinistrasse 52, 2000 Hamburg 20, Federal Republic of Germany

v

Local Organisation

Mannweiler, Klaus, Heinrich-Pette-Institut fuer experimentelle Vi­rologie und Immunologie an der Universitaet Hamburg, Martini­strasse 52,2000 Hamburg 20, Federal Republic of Germany Neth, Rolf, Universitaetskrankenhaus Eppendorf, Medizinische Kli­nik, Abteilung fuer klinische Chemie, Martinistrasse 52, 2000 Ham­burg 20, Federal Republic of Germany

VI

Contents

Participants of the Meeting 0

Wilsede Scholarship Holders Preface 0 0 0 0 0

Acknowledgment 0 0 0 0 0

Frederick Stohlman Jr. Memorial Lecture

XVII XXIII XXV

XXIX

Greaves, Mo Fo: Immunobiology of Lymphoid Malignancy 0 3

Thomas, Eo Do: Bone Marrow Transplantation in Leukemia 1 0 11

New Strategies in Diagnosis and Therapy

Hofibrand, Ao Vo, Ma, Do Do F., Prentice, Ho Go: Nucleotide Metabolism and Enzyme Inhibitors in Thymic Acute Lym-phoblastic Leukaemia 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 19

McCaffrey, Ro, Bell, Ro, Lillquist, A., Wright, Go, Baril, Eo, Mi­nowada, Jo: Selective Killing of Leukemia Cells by Inhibition ofTdT . 0 • 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 24

Papamichail, Mo, Trangas, T., Courtis, No, Ioannides, c., Cos­midou, Ho, Pangalis, Go A., Tsiapalis, C. Mo: Poly(A)-poly-merase Levels in Leukemia 0 0 0 • 0 0 0 0 0 0 • 0 0 0 0 28

Lister, T. A., Rohatiner, A. C. So, Slevin, Mo L., Dhaliwal, Ho So, Bell, Ro, Henry, Go, Thomas, Ho, Amess, Jo: Short-term Therapy for Acute Myelogenous Leukaemia in Younger Pa-tients 2

0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30

Hoelzer, Do, Thiel, Eo, Loffier, Ho, Bodenstein, Ho, BUchner, To, Messerer, Do, for the German Multicentre ALL! AUL Study Group: Multicentre Pilot Study for Therapy of Acute Lymphoblastic and Acute Undifferentiated Leukaemia in Adults 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36

Weinstein, Ho Jo, Mayer, Ro Jo, Rosenthal, Do So, Coral, Fo So, Camitta, B. Mo, Gelber, R. Do, Nathan, Do Go, Frei, Eo III: The Treatment of Acute Myelogenous Leukemia in Children andAduIts:VAPAUpdate 2

00000000000000 41

VII

Creutzig, u., Schellong, G., Ritter, J., Sutor, A H., Riehm, H., Langermann, H. J., Jobke, A, Kabisch, H.: Improved Results in Treatment of Acute Myelogenous Leukemia in Children -Report of the German Cooperative AML Study BFM-78 2 ., 46

Chesseis, J. M., Sieff, C. A, Rankin, A: Acute Myeloid Leu-kaemia in Childhood: Treatment in the United Kingdom 2 •• 51

Rohatiner, A Z. S., Balkwill, F., Lister, T. A: Interferon in Acute Myelogenous Leukaemia: A Preliminary Report . .. 56

Siegert, W., Huhn, D., Theml, H., Fink, U., Kaudewitz, P., Riethmuller, G., Wilmanns, W.: Human Interferon-Beta in the Treatment of Non-Hodgkin Lymphoma ....... 59

Zintl, F., Herman, J., Katenkamp, D., Malke, H., Plenert, W.: Results of LSA2-L2 Therapy in Children with High-Risk Acute Lymphoblastic Leukemia and Non-Hodgkin's Lym-phoma . . . . . . . . . . . . . . . . . . . . . . .. 62

Ramot, B., Ben-Bassat, I., Many, A, Kende, G., Neuman, Y., Brok-Simoni, F., Rosenthal, E.: Clinical and Epidemiological Observations on Acute Lymphoblastic Leukemia Subtypes at the Sheba Medical Center, Israel .......... 67

Slevin, M. L., Piall, E. M., Aherne, G. W., Johnston, A., Lister, T. A: The Clinical Pharmacology of Cytosine Arabi-noside . . . . . . . . . . . . . . . . . . . . . . .. 70

Erhardt, J., Emmerich, B., Theml, H., Riethmuller, G., Zieg­ler-Heitbrock, H. W. L.: Enhancement of the Cell-Mediated Lysis of Fresh Human Leukemia Cells by Cytostatic Drugs.. 76

Wilson, E. L., Jacobs, P., Dowdle, E. B.: Secretion of Plas­minogen Activators by Human Myeloid Leukemic Cells: Modulation and Therapeutic Correlations ........ 78

Wilms, K., Schuch, K., Muller, C., Link, H., Meyer, P., Osten­dorf, P., Waller, H. D., Wernet, P., Niethammer, D., Dopfer, H., Schneider, W., Schunter, F., Breitling, G., Frommhold, W., Seeger, G., Voss, A C., Rodt, H.: Bone Marrow Trans-plantation in West Germany in Patients with Leukemia 3. •• 81

Powles, R. L., Morgenstern, G. R., Leigh, M., Filshie, J., Platt, J., Lumley, H.: Cyc1osporinA Following Matched and Mis-matched Family Allogeneic Bone Marrow Transplants 2 • •• 87

Kaizer, H., Tutschka, P., Stuart, R., Korbling, M., Braine, H., Saral, R., Colvin, M., Santos, G.: Autologous Bone Marrow Transplantation in Acute Leukemia and Non-Hodgkin's Lymphoma: A Phase I Study of 4-Hydroperoxycyc1ophos­phamide (4HC) Incubation of Marrow prior to Cryopreser-vation 2. • . • • . • . . . . . . • • . . . . . • • •. 90

VIII

Rodt, Ho, Thierfelder, So, Bender-Gotze, Co, Dopfer, Ro, Haas, Fo Jo, Janka, Go, Kolb, Ho Jo, Link, Ho, Netzel, Bo, Nietham­mer, Do, Schiich, K., Wilms, K.: Serological Inhibition of Graft Versus Host Disease: Recent Results in 28 Patients with Leukemia 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Tutschka, Po Jo: Graft-Versus-Host Disease: Immunobiologi-cal Aspects 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Stiinkel, K., Bender-Gotze, c., Netzel, B., Thiel, Eo: T-Cell Phenotypes in Mixed Leukocyte Reactions and After Bone Marrow Transplantation: Are la-Antigen on T-Cells a Marker for GvH Reactions? 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Thorpe, Po Eo, Detre, So I., Mason, Do Woo Cumber, A Jo, Ross, Wo c.: Monoclonal Antibody Therapy: "Model" Exper­iments with Toxin-Conjugated Antibodies in Mice and Rats

LeBien, To Wo, Ash, R c., Zanjani, Eo Do, Kersey, Jo Ho: In Vitro Cytodestruction of Leukemic Cells in Human Bone Marrow Using a Cocktail of Monoclonal Antibodies

Ritz, Jo, Sallan, So Eo, Bast, R Co Jr., Lipton, Jo Mo, Nathan, Do Go, Schlossman, So Fo: In Vitro Treatment with Monoclonal Antibody Prior to Autologous Bone Marrow Transplantation in Acute Lymphoblastic Leukemia 2 0 0 0 0 0 0 0 0

Chen, Po-Mo, Ho, C.-Ko: Immunological Classification of Acute Lymphatic Leukemia 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Thoene, I., Kabisch, Ho: Multimarker Analysis of Childhood Acute· Lymphoblastic Leukemia (ALL): Heterogeneity of Cellular Phenotypes and Clinical Relevance of Immunologi-cal Defined ALL Subclasses 0 0 0 0 0 0 0

Crockard, A Do, O'Brien, Mo, Robinson, Do, Tavares de Castro, Jo, Matutes, Eo, Catovsky, Do: Morphological and C~tochemical Features of Adult T-Cell Lymphoma-Leukae-mIa 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Augener, Wo, Brittinger, Go, Schiphorst, Wo Eo C. Mo, van den Eijnden, Do Ho: Activities and Specificities of N-Acetylneur­aminyltransferases in Leukemic Cell 0 0 0 0 0 0 0 0 0 0 0

Tax, Wo Jo Mo, Greaves, Mo Fo, Willems, Ho Mo, Leeuwenberg, Ho Fo Mo, Capel, Po Jo A, Koene, R A Po: WTl: A Mono­clonal Antibody Reactive with T -ALL but not with Other Leukemias 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Bell, Ro, Lillquist, A, Cotter, So, Sallan, So, McCaffrey, R: Dysfunctional Glucocorticoid Receptors in Acute Leukemia 0

Galili, No, Galili, Uo: Glucocorticoid-Induced Lysis of Various Subsets of Acute Lymphoblastic Leukemia 0 0 0 0 0 0 0 0

92

97

102

107

112

117

124

126

131

135

139

142

146

IX

Blattner, W. A, Blayney, D. W., Jaffe, E. S., Robert-Guroff, M., Kalyanaraman, V. S., Gallo, R. c.: Epidemiology of HTL V -Associated Leukemia . . . ~. . . . . . . . . . . 148

Henderson, E. S.: Current Prospects for Clinical Care of Acute Leukemia. . . . . . . . . . . . . . . . . . . . 156

Mechanism of Malignant Transformation

Duesberg, P., Nunn, M., Biehl, T., Phares, W., Lee, W.-H.: Viral Oncogenes and Cellular Prototypes . . . . . . . . . 163

Bister, K., Enrietto, P., Graf, T., Hayman, M.: The Transform-ing Gene of Avian Acute Leukemia Virus MC29 ..... l73

Wong-Staal, F., Josephs, S., Dalla Favera, R., Westin, E., Gel­mann, E., Franchini, G., Gallo, R. c.: Cellular one Genes: Their Role as Progenitors of Viral one Genes and Their Ex­pression in Human Cells . . . . . . . . . . . . . . . . 178

Anders, F.: The Biology of an Oncogene, Based Upon Studies on Neoplasia in Xiphophorus . . . . . . . . . . . . . . 186

Papas, T. S., Rushlow, K. E., Watson, D. K., Bader, 1. P., Ray, D., Reddy, E. P.: The Transforming Gene of Avian Myelo­blastosis Virus (AMV): Nucleotide Sequence Analysis and Identification ofIts Translational Product ........ 207

Moelling, K., Greiser-Wilke, 1., Owada, M. K., Donner, P., Bunte, T.: DNA-Binding Ability of Transforming Proteins from Avian Erythroblastosis Virus and Mutant Avian Myelo­cytomatosis Virus, MC29, in Comparison with MC29 Wild Type ........................ 214

Kettmann, R., Westin, E. H., Marbaix, G., Deschamps, J., Wong-Staal, F., Gallo, R. c., Bumy, A: Lack of Expression of Cellular Homologues of Retroviral one Genes in Bovine Tumors .. . . . . . . . . . . . . . . . . . . . . . 218

Bruck, c., Portetelle, D., Zavada, J., Bumy, A: Molecular Dissection of the Bovine Leukemia Virus Envelope Glycopro-tein (BL V gp5l) by a Monoclonal Antibody Study 222

Chen, A P., Essex, M., de Noronha, F.: Detection and Locali­zation of a Phosphotyrosine-Containing one Gene Product in Feline Tumor Cells . . . . . . . . . . . . . . . . . . 227

Scott, M. R. D., Brickell, P. M., Latchman, D. S., Murphy, D., Westphal, K.-H., Rigby, P. W. J.: The Use of cDNA Cloning Techniques to Isolate Genes Activated in Tumour Cells 236

Lane, M. A, Sainten, A., Neary, D., Becker, D., Cooper, G. M.: Cellular Transforming Genes in Cancer ....... 241

x

Dalla Favera, R., Westin, E., Gelmann, E. P., Martinotti, S., Bregni, M., Wong-Staal, F., Gallo, R. c.: The Human one Gene e-mye: Structure, Expression, and Amplification in the Human Promyelocytic Leukemia Cell Line HL-60 247

Haseltine, W. A., Lenz, J., Crowther, R.: Determination of Virulence Properties of Leukemia Viruses ........ 255

Fischinger, P. J., Dunlop, N. M., Poore, C. M., Robey, W. G.: Generation of Recombinant Murine Leukemia Viruses De Novo: An Alternative Model for Leukemogenesis ..... 261

Jaenisch, R., Harbers, K., Jahner, D., Stewart, C., Stuhlmann, H.: Expression of Retroviruses During Early Mouse Embryo­genesis . . . . . . . . . . . . . . . . . . . . . . . . 270

Chieco-Bianchi, L., D'Andrea, E. D., De Rossi, A., Zanovello, P., Ronchese, F., Collavo, D.: Natural History of M-MSV Tumors in Mice Carrying Endogenized Moloney Leukemia Virus. . . . . . . . . . . . . . . . . . . . . . . . . 275

Nobis, P., L6hler, J.: Induction of Histiocytomas by Pristane Treatment of Mice Chronically Infected with Moloney Mu-rine Leukemia Virus . . . . . . . . . . . . . . . . . . 280

Jaquemin, P. c.: Purification of a Reverse Transcriptase-like Protein from the Plasma of a Patient with Chronic Myeloge-nous Leukemia and Production of Monoclonal Antibodies 282

Schetters, H., ErfIe, V., Hehlmann, R.: Detection of Group and Interspecies Reactivities in Mammalian C-Type Virus p30 Proteins and Corresponding Human Antigens 284

Hausen, H. zur: Papovaviruses and Human Tumors 289

Modrow, S., Schmidt, H., Wolf, H.: Characterization of Pro-teins Induced by Herpesvirus saimiri: Comparative Immuno­precipitation and Analysis of Glycosylation . . . . . . . . 293

Bornkamm, G. W., Freese, U. K., Laux, G., Hudewentz, J., Delius, H.: Structural Organization and Expression of the Ep­stein-Barr Virus Genome . . . . . . . . . . . . . . . . 295

Bauer, G., Hausen, H. zur: Induction of Latent Epstein-Barr Virus Information by a Serum Factor .......... 297

Boniver, J., Houben-Defresne, M. P.: Thymic Nurse Cells and Radiation Leukemia Virus Induced Thymic Lymphomas in C57BL Mice . . . . . . . . . . . . . . . . . . . . . 299

Young, B. D., Jeanpierre, M., Goyns, M. H., Stewart, G. D., Elliot, T., Krumlauf, R.: Construction and Characterization of Chromosomal DNA Libraries . . . . . . . . . . . . . . 301

XI

Gallo, R. c., Popovic, M., Sarin, P., Reitz, M. S. Jr., Kalyana­raman, V. S., Aoki, T., Sarngadharan, M. G., Wong-Staal, F.: Human T-Cell Leukemia-Lymphoma Virus (HTLV): A Pro-gress Report . . . . . . . . . . . . . . . . . . . . . 311

Metcalf, D.: Early Events in the Suppression of Myeloid Leu-kemic Cells by Biological Regulators .......... 320

Moore, M. AS., Gabrilove, J., Sheridan, A P.: Myeloid Leu­kemic Cell Differentiation Induced by Human Postendotoxin Serum and Vitamin Analogues ............. 327

Burgess, A W., Cooper, P. c., Stanley, I. J., Nicola, N. A: Ef-fect of Colony-stimulating Factors on the Proteins Synthe-sized by Normal and Leukemic Myeloid Progenitor Cells 338

Nicola, N. A, Matsumoto, M., Metcalf, D., Johnson, G. R.: Molecular Properties of a Factor Inducing Differentiation in Murine Myelomonocytic Leukemic Cells . . . . . . . . . 345

Minowada, J., Minato, K., Tatsumi, E., Sugimoto, T., Nakaza-wa, S., Ohnuma, T., Kubonishi, I., Miyoshi, I., Frankel, A, Gallo, R. c.: A Model Scheme for Human Hematopoietic Cell Differentiation as Determined by Multiple Markers of Leukemia-Lymphomas ................ 348

Singer, J. W., Keating, A.: Studies on the In Vitro Microenvi­ronment in Man . . . . . . . . . . . . . . . . . . . . 351

Francis, G. E., Guimaraes, J. E., Berney, J. J., Granger, S., Hoflbrand, A V.: Granulocyte-Macrophage Progenitor Cell Proliferation and Differentiation . . . . . . . . . . . . . 355

Elstner, E., Schulze, E., Ihle, R., Schlitt, M., Stobbe, H.: Proli­feration and Maturation of Hemopoietic Cells from Patients with Preleukemia and Aplastic Anemia in Agar and Diffusion Chamber Cultures . . . . . . . . . . . . . . . . . . . 358

Rabe, K., Rehpenning, W., Winkler, K., Heinisch, B., Krause, U., Soltau, H., Neth, R.: Persistent Deficiency of Myeloper­oxidase and Lactoferrin in Granulopoietic Cells of Patients with Acute Leukemia ................. 362

Izaguirre, C. A, Greaves, M. F.: Studies on Normal B-Cells and Common Acute Lymphoblastic Leu~emia Blast Cells Using a Colony Assay . . . . . . . . . . . . . . . . . 366

Welte, K., Venuta, S., Wang, C. Y., Feldman, S. P., Ciobanu, N., Kruger, G., Feickert, H. J., Merluzzi, V. J., Flomenberg, N., Moore, M. AS., Mertelsmann, R.: Human Interleukin 2: Physiology, Biochemistry, and Pathophysiology in Lympho-blastic Leukemias and Immunodeficiency Syndromes 369

Brown, G., Fisher, A G., Bunce, C. M., Stone, P. C. W., Tok-soz, D.: Studies of Myelopoiesis Using Monoclonal Anti­bodies and Variant Lines from the Pro myeloid Cell Line HL60 . . . . . . . . . . . . . . . . . . . . . . . . 380

XII

Olsson, I. L., Breitman, T. R., Sarngadharan, M. G., Gallo, R. c.: Mechanisms for Induction of Differentiation in the Hu-man Promyelocytic Cell Line HL-60' . . . . . . . . . . 384

Uchailska-Ziegler, B., Wernet, P., Ziegler, A: Differentiation of a Human Myeloid Cell Line (HL-60) Toward Granulocyte­and Macrophage-like Cells: Comparison of Cell Surface Anti-gen Expression . . . . . . . . . . . . . . . . . . . . 386

Sarin, P. S., Popovic, M., Salahuddin, S. Z., Richardson, E., Lange Wantzin, G., Karmarsky, B., Gallo, R. c.: Transmis­sion of Human T-Cell Leukemia Virus (HTLV) into Human Cord Blood T Cells .................. 389

Lau, B., Jager, G., Korge, E., Dormer, P.: K 562 Cell Line in Plasma Clot Diffusion Chambers: Changes in Cell Surface Phenotype in Relationship to Culture Conditions ..... 394

Sieff, c., Bicknell, D., Caine, G., Edwards, P. A W., Greaves, M.: Antigen Expression on Normal and Leukaemic Erythroid Precursors. . . . . . . . . . . . . . . . . . . . . . . 397

Peschle, c., Rossi, G. B., Covelli, A, Migliaccio, G., Migliaccio, A. R., Mastroberardino, G.: The Early Stage of Friend Virus Erythroleukemias: Mechanisms Underlying BPA-"indepen-dent" In Vitro Growth ofBFU-E ............ 403

Stein, H., Gerdes, J., Schwab, U., Lemke, H., Diehl, V.: Evi­dence for the Origin of Hodgkin and Sternberg-Reed Cells from a Newly Detected Small Cell Population 407

Diehl, V., Burrichter, H., Schaadt, M., Kirchner, H. H., Fonatsch, c., Stein, H., Gerdes, J., Heit, W., Ziegler, A: Hodgkin's Disease Cell Lines: Characteristics and Biological Activities . . . . . . . . . . . . . . . . . . . . . . . 411

Lennert, K., Schmid, u.: Prelymphoma, Early Lymphoma, and Manifest Lymphoma in Immunosialadenitis (Sjogren's Syndrome) - A Model of Lymphomagenesis ....... 418

Immunological Aspects in Malignancy

Cooper, M. D., Kubagawa, H.: B-Cell Malignancies: Origin and Extent of Clonal Involvement ........... 425

Fortunato, A, James, R. F. L., Mellor, A, Mitchison, N. A: Transfection as an Approach to Understanding Membrane Glycoproteins .................... 434

Terhorst, c., Borst, J., Lerch, P., van de Rijn, M., Snow, P., Spits, H., de Vries, J.: Structural and Functional Aspects of the T-Cell Differentiation Antigens T3, T6, and T8 440

Ploegh, H. L.: Human Histocompatibility Antigens 447

XIII

Weiss, E. H., Mellor, A L., Golden, L., Bud, H., Hurst, J., Flavell, R. A., Simpson, E., James, R., Townsend, A. R. M., Festenstein, H.: Structure and Expression of Class I Genes of the Mouse Major Histocompatibility Complex ...... 454

Reitz, M. S., Mann, D., Clarke, M. F., Kalyanaraman, V. S., Robert-Guroff, M., Popovic, M., Gallo, R. c.: Presence of HTL V in a Subset of T Cells from an Infected Patient: Some Immunochemical Properties of the Infected Cells 459

Ziegler, A, Uchanska-Ziegler, B., Wernet, P., Zeuthen, J.: Hybrids Between Human Cell Lines Belonging to Different Hematopoietic Pathways: Analysis of HLA and Myeloid Sur-face Antigens . . . . . . . . . . . . . . . . . . . . . 462

Gidlund, M., Nilsson, K., Totterman, T., Wigzell, H.: Natural Killer Cells and Their Targets: Impact of Differentiation on Target Cell Susceptibility . . . . . . . . . . . . . . . . 466

Ehrlich, R., Efrati, M., Gonen, B., Shochat, L., Witz, I. P.: Na­tural Cellular Defense Activities Against Tumors - Cytostasis and NK Activity . . . . . . . . . . . . . . . . 470

Vodinelich, L., Schneider, c., Sutherland, D. R., Newman, R. A, Greaves, M. F.: Structure and Function of the Transferrin Receptor - A Possible Role in the Recognition of Natural Killer Cells . . . . . . . . . . . . . . . . . . 472

Milleck, J., Jantscheff, P., Irro, F., Tkatscheva, N., Le Dinh Hoe: NK and K Cells in Malignant Lymph Nodes 475

Green, D. R., Gershon, R. K.: Contrasuppression, Class I An-tigens, and Cancer Immunity . . . . . . . . . . . 479

Flood, P. M., DeLeo, A B., Old, L. J., Gershon, R. K.: Inhibi-tion of the Induction of Contrasuppression by Antisera Against Tumor-Associated Surface Antigens on Methyl­cholanthrene-induced Sarcomas . . . . . . . . . . . . . 486

Wortzel, R. D., Philipps, c., Urban, J. L., Schreiber, H.: Dis­section of a Unique Tumor-Specific Transplantation Antigen into Multiple Unique Independent Epitopes using Syngeneic T -Cell Lines . . . . . . . . . . . . . . . . 489

Rohmer, H., Schetters, H., Luz, A., Hehlmann, R., Erfie, V.: Detection of Antigen-(AKR MuL V gp70)-Specific Circulating Immune Complexes (CIC) in Mice with Lymphomas 493

Sarngadharan, M. G., Schuepbach, J., Kalyanaraman, V. S., Robert-Guroff, M., Oroszlan, S., Gallo, R. c.: Immunological Characterization of the Natural Antibodies to Human T-Cell Leukemia Virus in Human Sera ............ 498

XIV

General Summary

Levy, 10 Po: General Summary of the Meeting 507

Subject Index 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 515

1 Also special Lecture for the Wilsede Joint Meeting on Pediatric Oncolo­gy II

2 Were also presented in the Wilsede Joint Meeting on Pediatric Oncology II 3 Presented in the Wilsede Joint Meeting on Pediatric Oncology II

xv

Participants of the Meeting

Anders, Fritz, Genetisches Institut der Universitaet, Heinrich-ButT-Ring 58-62,6300 Giessen, Federal Republic of Germany

Bauer, Georg, Institut fuer Virologie im Zentrum fUr Hygiene, Hermann­Herder-Strasse 11,7800 Freiburg, Federal Republic of Germany

Bell, Richard, Medical Oncology University Hospital, 75 East Newton Street, Boston, MA 02062, USA

Bernhard, Silke, Dahlem-Konferenzen, Wallotstrasse 19, 1000 Berlin 33, Federal Republic of Germany

Bister, Klaus, Max-Planck-Institut fUr Molekulare Genetik, Ihnestrasse 63-73, 1000 Berlin 33, Federal Republic of Germany

Blattner, William A., Family Studies Section, Environmental Epidemiology Branch, National Cancer Institute, Landow Building, Rm. 4C18, 7910 Woodmont Avenue, Bethesda, MD 20205, USA

Boiron, Michel, Institut de Recherches sur les Leucemies et les Maladies du Sang, Universite Paris VII, Hopital Saint-Louis, 2 Place du Docteur-Four­nier, 75475 Paris Cedex 10, France

Boniver, Jacques, Institut de Pathologie B 23, Laboratoire d'Anatomie, Pathologique, 4000 Liege, Belgium

Bornkamm, Georg W., Institut fuer Virologie im Zentrum fuer Hygiene, Hermann-Herder-Strasse 11,7800 Freiburg, Federal Republic of Germany

Burgess, Antony W., Ludwid Institute for Cancer Research, Melbourne Tu­mour Biology Unit, Royal Melbourne Hospital, Victoria 3050, Australia

Burny, Arsene, Faculte des Sciences, Laboratoire de Chimie Biologique, 67 Rue des Chevauc, 1640 Rhode-St-Genese, Belgium

Chen, Po-min, Veterans General Hospital, Dept. of Hematology, Taipei, Tai­wan 112, Republic of China

Chessels, Judith M., The Hospital for Sick Children, Dept. of Haematology and Oncology, Great Ormond Street, London WCIN3JH, Great Britain

Chieco-Bianchi, Luigi, University of Padova, Dept. of Oncology, Via A. Ga­belli 61, 351000 Padova, Italy

Cooper, Max D., UAB, Comprehensive Cancer Center, Cellular Immuno­biology Unit, Dept. of Pediatrics and Microbiology, Birmingham, AL 35294, USA

Creutzig, Ursula, Universitaets-Kinderklinik, Robert-Koch-Strasse 31, 4400 Muenster, Federal Republic of Germany

Dalla Favera, Riccardo, National Cancer Institute, Building 37, Rm. 6 C04, Bethesda, MD 20014, USA

Dautry, Fran<,:ois, MIT, Center for Cancer Research, 77 Massachusetts Ave­nue, Boston, MA 02139, USA

Deinhardt, Friedrich, Max-v.-Pettenkofer-Institut, Pettenkoferstrasse 9a, 8000 Muenchen 2, Federal Republic of Germany

Diehl, Volker, Medizinische Hochschule Hannover, Zentrum Innere Medizin und Dermatologie, Karl-Wiechert-Allee 9, 3000 Hannover 61, Federal Re­public of Germany

XVII

Duesberg, Peter, Dept. of Molecular Biology, University of California, Wen­dell M. Stanley Hall, Berkeley, CA 94720, USA

Ehrlich, Rachel, George S. Wise Faculty of Life Sciences, Dept. of Microbio­logy, Tel Aviv, Israel

Elstner, Elena, Universitaetsklinik fuer Innere Medizin, Charite, Schumann­strasse 20121, 1040 Berlin, German Democratic Republic

Erfie, Volker, Gesellschaft fuer Strahlen- und Umweltschaeden, Institut flir Biologie, 8042 Neuherberg, Federal Republic of Germany

Essex, Max, Harvard School of Public Health, Dept. of Microbiology, 665 Huntington Avenue, Boston, MA 02115, USA

Ferrari, Sergio, Instituto di Clinica Medica, Policlinico, Via del Pozzo 71, 41100 Modena, Italy

Fischinger, Peter J., National Cancer Institute, Building 31, Rm. llA56, Bethesda, MD 20014, USA

Freudenstein, Christa, Institut fuer Virusforschung, DKFZ, 1m Neuenheimer Feld 280,6900 Heidelberg, Federal Republic of Germany

Galili, Naomi, Hadassah Medical School, Dept. of Virology, Jerusalem, Israel

Gallo, Robert C., National Cancer Institute, Building 37, Rm. 6B04, Bethes­da, MD20014, USA

Galton, David A. G., MRC, Leukaemia Unit, Hammersmith Hospital, Du­cane Road, London EI20HS, Great Britain

Gershon, Richard K., Yale University, Dept. of Pathology, 310 Cedar Street, New Haven, CT 06510, USA

Gianni, Alessandro M., Instituto di Clinica Medica I, Padiglione Granelli, Via Francesso Sforza 35, 20122 Milano, Italy

Goldblum, Natan, Hadassah Medical School, Dept. of Virology, Jerusalem, Israel

Grandori, Carla, Instituto Suoeriore di Sanita, Laboratorio Pathologia non Infettiva, Viale Regina Elena 299, 00161 Rome, Italy

Greaves, Melvyn F., Imperial Cancer Research Fund Laboratories, Mem­brane Immunology Laboratory, Lincoln's Inn Fields, London WC2A3PX, Great Britain

Haseltine, William A., Sidney Farber Cancer Institute, The Jimmy Fund, 45 Binney Street, Boston, MA 02115, USA

Hausen, Harald zur, Institut fuer Virologie im Zentrum fuer Hygiene, Her­mann-Herder-Strasse 11,7800 Freiburg, Federal Republic of Germany

Hehlmann, Riidiger, Medizinische Poliklinik der Universitaet Muenchen, Pettenkoferstrasse 8 a, 8000 Muenchen 2, Federal Republic of Germany

Heimpel, Hermann, Zentrum fuer Innere Medizin und Kinderheilkunde der Universitaet Ulm, Steinhoevelstrasse 9, 7900 UIm, Federal Republic of Germany

Helm, Klaus von der, Max-v.-Pettenkofer-Institut, Pettenkoferstrasse 9 a, 8000 Muenchen 2, Federal Republic of Germany

Henderson, Edward S., Roswell Park Memorial Institute, Medicine A, 666 EIm Street, Buffalo, NY 14263, USA

Hoelzer, Dieter, Zentrum fuer Innere Medizin und Kinderheilkunde der Universitaet, Steinhoevelstrasse 9, 7900 Ulm, Federal Republic of Ger­many

HofIbrand, A. Victor, The Royal Free Hospital, Dept. of Haematology, Pond Street, Hampstead, London NW32QG, Great Britain

Hofschneider, Peter-Hans, Max-Planck-Institut fuer Biochemie, 8033 Mar­tinsried, Federal Republic of Germany

Hossfeld, Dieter Kurt, Medizinische Universitaetsklinik, Abteilung Onkolo­gie/Haematologie, Martinistrasse 52, 2000 Hamburg 20

Izaguirre, Carlos, Imperial Cancer Research Fund Latoratories, St. Bartholo­mew's Hospital, West Smithfield, London ECIA7BE, Great Britain

Jacquemin, Paul, Institut Ludwig de Recherches sur Ie Cancer, Avenue Hip­pocrate 74, UCL 7459, 1200 Brussels, Belgium

XVIII

Jaenisch, Rudolf, Heinrich Pette-Institut fuer experimentelle Virologie und Immunologie, Martinistrasse 52, 2000 Hamburg 20, Federal Republic of Germany

Kaplan, Henry S., Stanford University Medical Center, Dept. of Radiology, Cancer Biology Research Laboratory, Stanford, CA 94305, USA

Kersey, John H., Dept. of Laboratory Medicine, 420 Delaware Street S.E., Box 609 - Mayo, Minneapolis, MN 55455, USA

Kisseljov, Fjodor L., Cancer Research Center, Kashirskoie Shosse 6, Moscow 115478, USSR

Konze-Thomas, Beate, Deutsche Forschungsgemeinschaft, Kennedyallee 40, 5300 Bonn-Bad Godesberg, Federal Republic of Germany

Kramer, Gisela, Clayton Foundation Biochemical Institute, Dept. ofChemis­try, Austin, TX 78712, USA

Kurth, Reinhard, Friedrich-Miescher-Laboratorium der Max-Planck-Gesell­schaft, Spemannstrasse 37-39, 7400 Tuebingen, Federal Republic of Ger­many

L'Age-Stehr, Johanna, Robert-Koch-Institut des Bundesgesundheitsamtes, Abteilung Virologie, Nordufer 20, 1000 Berlin 65, Federal Republic of Germany

Lane, Mary-Ann, DANA 940A, Laboratory of Molecular Carcinogenesis, Sidney Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA

Lau, Barbara, Institut fuer Haematologie, Abteilung Experimentelle Haema­tologie, Landwehrstrasse 61, 8000 Muenchen 2, Federal Republic of Ger­many

Lennert, Karl, Institut fuer Pathologie, Abteilung Allgemeine Pathologie und Pathologische Anatomie, Hospitalstrasse 42, 2300 Kiel, Federal Republic of Germany

Levy, Jean-Paul, Laboratoire Immunologie et Virologie des Tumeurs, Hopi­tal Cochin, Groupe INSERM U152, 27 Rue du Foubourg Saint-Jacques, 75674 Paris Cedex 14, France

Lister, T. Andrew, Imperial Cancer Research Fund Laboratories, Dept. of Medical Oncology, St. Bartholomew's Hospital, West Smithfield, London ECIA7BE, Great Britain

Mannweiler, Klaus, Heinrich-Pette-Institut fuer experimentelle Virologie und Immunologie, Martinistrasse 52, 2000 Hamburg 20, Federal Republic of Germany

Mertelsmann, Roland, Memorial Sloan-Kettering, Cancer Center, 1275 York Avenue, New York, NY 10021, USA

Metcalf, Donald, The Walter and Eliza Hall Institute of Medical Research, Cance Research Unit, Royal Melbourne Hospital, Victoria 3050, Australia

Milleck, Jiirgen, Zentralinstitut flir Krebsforschung, Lindenberger Weg 80, 1115 Berlin-Buch, German Democratic Republic

Miller, Denis R., Memorial Sloan-Kettering, Cancer Center, 1275 York Ave­nue,NewYork,NY 10021, USA

Minowada, Jun, Roswell Park Memorial Institute, 666 Elm Street, Buffalo, NY 14263, USA

Mitchison, N. Avrion, University College London, Tumour Immunology Unit, Gower Street, London WC1E6BT, Great Britain

MoIling, Karin, Max-Planck-Institut fuer Molekulare Genetik, Ihnestrasse 63-73, 1000 Berlin 33, Federal Republic of Germany

Moore, Malcolm A. S., Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA

Munk, Klaus, Institut fuer Virusforschung, DKFZ, 1m Neuenheimer Feld 280,6900 Heidelberg, Federal Republic of Germany

Myers, Richard M., Harvard University, Cambridge, MA 02248, USA Neth, Rolf-Dietmar, Abteilung fuer Klinische Chemie der Medizinischen

Klinik, Universitaetskrankenhaus Eppendorf, Martinistrasse 62, 2000 Hamburg 20, Federal Republic of Germany

Nicola, Nicos A., The Walter and Eliza Hall Institute of Medical Research, Cancer Research Unit, Royal Melbourne Hospital, Victoria 3050, Aus­tralia

XIX

Olsson, Inge L., Research Department 2, E-blocket, University Hospital, 221 85 Lund, Sweden

Papamichail, Michai!, Hellenic Anticancer Institut, 171, Alexandras Ave, Athen, Greece

Papas, Takis S., Carcinogenesis Regulation Section, Laboratory of Molecular Oncology, NIH, Building 41, Suite 400, Bethesda, MD 20014, USA

Peschle, Cesare, Instituto Superiore di Sanita, Laboratorio Patologia non In­fettiva, Viale Regina Elena 299, 00161 Rome, Italy

Pimentel, Enrique, Instituto de Medicina Experimentas, Apartado Postal 50587, Caracas 1050-A, Venezuela

Pinkel, Donald, Sunny and Isadore Familian Children's Hospital, City of Hope National Medical Center, 1500 East Duarte Road, Duarte, CA 91010, USA

Ploegh, Hidde L., Institut fuer Genetik der Universitaet zu Koeln, Weyertal 121,5000 Koeln 41, Federal Republic of Germany

Pragnell, Ian B., The Beatson Institute for Cancer Research, Wolfson Labo­ratory for Molecular Pathology, Garscube Estate, Switchback Road, Bears­den, Glasgow G6llBD, Great Britain

Ramot, Bracha, Chaim Sheba Medical Center, Institute of Hematology, Tel Hashomer, Israel

Reitz, Marvin, National Cancer Institute, Building 37, Rm. 6C09, Bethesda, MD20014, USA

Riethmiiller, Gert, Institut fuer Immunologie, Schillerstrasse 42, 8000 Muen­chen 2, Federal Republic of Germany

Rigby, Peter W. J., Imperial College of Science and Technology, Dept. of Biochemistry, London SW72AZ, Great Britain

Rodt, Hans, Institut fuer Haematologie, Abteilung Immunologie, Landwehr­strasse 61, 8000 Muenchen 2, Federal Republic of Germany

Rohatiner, Ama, Imperial Cancer Research Fund Laboratories, Dept. of Me­dical Oncology, St. Bartholomew's Hospital, West Smithfield, London EC1A7BE, Great Britain

Sarin, Prem S., National Cancer Institute, Building 37, Rm. 6A09, Bethesda, MD 20014, USA

Sarngadharan, Mangallasserie, National Cancer Institute, Building 37, Rm. 6A09, Bethesda, MD 20014, USA

Schreiber, Hans, La Rabida Children's Hospital and Research Center, Dept. of Pathology, East 65 Street at Lake Michigan, Chicago, IL 60649, USA

Schubert, Johannes, St.-Joseph-Hospital, Wiener Strasse 1, 2050 Bremer­haven, Federal Republic of Germany

Simon, Markus, Max-Planck-Institut fuer Immunbiologie, Stuebeweg 51, 7800 Freiburg-Zaehringen, Federal Republic of Germany

Singer, Jack W., Veterans Administration Medical Center, 4435 Beacon Ave­nue South, Seattle, W A 98108, USA

Slevin, Maurice, Imperial Cancer Research Fund Laboratories Dept. of Me­dical Oncology, St. Bartholomew's Hospital, West Smithfield, London ECIA7BE, Great Britain

Stein, Harald, Institut fuer Pathologie, Abteilung Allgemeine Pathologie und Pathologische Anatomie, Hospitalstrasse 42, 2300 Kiel, Federal Republic of Germany

Tax, Wi!, St. Radboudziekenhuis, Klinik voor Inwendige Ziekten, Afdeling Nephrologie, Geert Grooteplein zuid 8, 6500 HB Nijmegen, The Nether­lands

Terhorst, Cox, Sidney Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA

Thomas, E. Donnall, The Fred Hutchinson Cancer Research Center, Divi­sion of Oncology, Dept. of Medicine, 1124 Columbia Street, Seattle, W A 98104, USA

Thorpe, Philip E., Imperial Cancer Research Fund Laboratories, Holburn, London WC2A3PX, Great Britain

Torelli, Giuseppe, Instituto di Clinica Medica, Policlinico, Via del Pozzo 71, 41100 Modena, Italy

xx

Torelli, Umberto, Instituto di Clinica Medica, Policlinico, Via del Pozzo 71, 41100 Modena, Italy

Uchanska-Ziegler, Barbara, Medizinische Klinik, Abteilung Innere Medi­zin II, Immunologie-Labor, Otfried Mueller-Strasse, 7400 Tuebingen

Vande Woude, George F., NIH, Building 41, Rm. 100, Laboratory of Mole­cular Oncology, Bethesda, MD 20205, USA

Vondelich, Laida, Imperial Cancer Research Fund Laboratories, Lincoln's Inn Fields, London WC2A3PX, Great Britain

Watt, Suzanne, Dept. of Pathology, Division ofImmunology, Addenbrooke's Hospital, Hills Road, Cambridge CB22QQ, Great Britain

Weinstein, Howard l., Sidney Farber Cancer Institute, Dept. of Pediatric Oncology, 44 Binney Street, Boston, MA 02115, USA

Weiss, Elisabeth, National Institute for Medical Research, The Ridgeway, London NW7IAA, Great Britain

Weiss, Robin A., Institute of Cancer Research, Royal Cancer Center, Chester Beatty Research Institute, Fulham Road, London SW36JB, Great Britain

Wigzell, Hans, Biomedicinska Centrum, Avdelingen foer Immunologi, 75123 Uppsala, Sweden

Wilson, Elaine L., University of Cape Town, Dept. of Clinical Science and Immunology, Observatory, 7925 Cape, South Africa

Wolf, Hans, Max-v.-Pettenkofer-Institut, Arbeitsgruppe Molekulare Virolo­gie, Pettenkoferstrasse 9 a, 8000 Muenchen 2, Federal Republic of Ger­many

Wong-Staal, Flossie, National Cancer Institute, Building 37, Bethesda, MD 20014, USA

Young, Brian, The Beatson Institute for Cancer Research, Garscube Estate Switchback Road, Bearsden, Glasgow G6IIBD, Great Britain

Zintl, Felix, Universitaets-Kinderklinik, Kochstrasse 2, 6900 lena, German Democratic Republic

XXI

Wilsede Scholarship Holders

(Granted by the Deutsche Krebshilfe, Leukemia Research Fund Great Britain, and the Leukemia Society of America)

Andreesen, Reinhard, Medizinische Universitaetsklinik, Abteilung Innere Medizin I, Hugstetter Strasse 55, 7800 Freiburg, Federal Republic of Ger­many

Augener, Wiprecht, Medizinische Klinik, Haematologische Abteilung, Hufe­landstrasse 55, 4300 Essen, Federal Republic of Germany

Becker, Thomas, Kinder-Poliklinik, Feulgenstrasse 12,6300 Giessen, Federal Republic of Germany

Geoffrey, Brown, Dept. of Immunology, Vincent Drive, Birmingham B 1 52TJ, Great Britain

Crockard, Alistair D., Dept. of Haematology, Royal Victoria Hospital, Bel­fast BTl26BA, Northern Ireland

Dolken, Gottfried, Medizinische Universitaetsklinik, Hugstetter Strasse 55, 7800 Freiburg, Federal Republic of Germany

Drexler, H. Gunter, Zentrum fuer Kinderheilkunde der Universitaet Ulm, Prittwitzstrasse 43, 7900 Ulm, Federal Republic of Germany

Erhardt, Johannes, Institut fUr Immunologie, Schillerstrasse 42, 8000 Muen­chen 2, Federal Republic of Germany

Flood, Patrick M., Yale University, Dept. of Pathology, Brady Memorial La­boratory, FMB 202,210 Cedar Street, New Haven, CT 06510, USA

Francis, Gillian, The Royal Free Hospital, Dept. of Haematology, Pond Street, London NW32QG, Great Britain

Ganser, Arnold, Abteilung fuer Klinische Physiologie und Arbeitsmedizin, Oberer Eselsberg, 7900 Ulm, Federal Republic of Germany

Gassmann, Winfried, II. Medizinische Klinik, Metzstrasse 53/57, 2300 Kiel, Federal Republic of Germany

Haas, Wolfgang, Pathologisches Institut, Joseph-S.telzmann-Strasse 9, 5000 Koeln 41, Federal Republic of Germany

Haworth, Catherine, Paterson Laboratories, Christie Hospital, Holt Radium Institute, Withington, Manchester M209BX, Great Britain

Heckmayr, Marlene, II. Medizinische Klinik, Abteilung Onkologie und Haematologie, Universitaetskrankenhaus Eppendorf, Martinistrasse 52, 2000 Hamburg 20, Federal Republic of Germany

Hiddemann, Wolfgang, Medizinische Klinik Abteilung Innere MedizinA, Westring 3, 4400 Muenster, Federal Republic of Germany

Hirsch, Friedrich, Medizinische Universitaetsklinik, Hugstetter Strasse 55, 7800 Freiburg, Federal Republic of Germany

Hofer, Paul, Institut fuer Immunologie, Schillerstrasse 42, 8000 Muen­chen 2, Federal Republic of Germany

Jager, Gundula, Institut fuer Haematologie, Landwehrstrasse 61, 8000 Muenchen 2, Federal Republic of Germany

Jendis, Jorg, Max-Planck-Institut fuer Biochemie, Abteilung Hofschneider, 8033 Martinsried, Federal Republic of Germany

Kanz, Lothar, Medizinische Universitaetsklinik, Abteilung Haematologie und Onkologie, Hugstetter Strasse 55, 7800 Freiburg, Federal Republic of Germany

XXIII

Mills, K., University College London, Dept. of Haematology, Gower Street, London WCIE6AU, Great Britain

Miiller, Dorith, Institut und Poliklinik fuer Klinische Immunologie und Rheumatologie, Krankenhausstrasse 12, 8520 Erlangen, Federal Republic of Germany

Mullins, Jim, Harward School of Public Health, Dept. of Microbiology, 66 Huntington Avenue, Boston, MA 02115, USA

Murray, Lesley, Imperial Cancer Research Fund Laboratory, Dept. ofMedi­cal Oncology, St. Bartholomew's Hospital, West Smithfield, London ECIA7BE, Great Britain

Nobis, Peter, Abteilung Molekularbiologie, Physiologisch-Chemisches Insti­tut, Grindelallee 117,2000 Hamburg 13, Federal Republic of Germany

Porzsolt, Franz, Zentrum fuer Innere Medizin der Universitaet UIm, Stein­hoevelstrasse 9, 7900 VIm, Federal Republic of Germany

Ritter, Jorg, Kinderklinik, Abteilung Haematologie und Onkologie, Robert­Koch-Strasse, 4400 Muenster, Federal Republic of Germany

Riibsamen, Helga, Paul-Ehrlich-Institut, Bundesamt fuer Sera und Impfstof­fe, Paul-Ehrlich-Strasse 42-44, 6000 Frankfurt, Federal Republic of Ger­many

Schlimok, Giinter, II. Medizinische Klinik, Langemarckstrasse 11, 8900 Augsburg, Federal Republic of Germany

Scott, Martin, Stanford University Medical Center, Dept. of Radiology, Can­cer Biology Research Laboratory, Stanford, CA 94305, USA

Sieber, Gerhard, Universitaetsklinikum Steglitz, Abteilung Innere Medizin, Haematologie und Onkologie, Hindenburgdamm 30, 1000 Berlin 45, Fed­eral Republic of Germany

Sieff, Colin, The Hospital for Sick Children, Dept. of Haematology, Great Ormond Street, London WCIN3JH, Great Britain

Siegert, Wolfgang, Medizinische Klinik III, Klinikum GroBhadern, 8000 Muenchen 70, Federal Republic of Germany

Strobel, Eva-Susanne, Husserlstrasse 4, 7800 Freiburg, Federal Republic of Germany

Stiinkel, Klaus, Institut fliT Haematologie, Abteilung Immunologie, Land­wehrstrasse 61, 8000 Muenchen 2, Federal Republic of Germany

Thone, Ingo, Universitaets-Kinderklinik, Martinistrasse 52, 2000 Ham­burg 20, Federal Republic of Germany

Welte, Karl, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA

Wilkie, N., The Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G6IIBD, Great Britain

Ziegler, Andreas, Medizinische Universitaetsklinik, Labor fuer Immunologe, 7400 Tuebingen, Federal Republic of Germany

Zigler, Hans-Werner L., Institut fUr Immunologie, Schillerstrasse 42, 8000 Muenchen 2, Federal Republic of Germany

Ziemiecki, Andrew, Institut fuer Virologie, Frankfurter Strasse 107, 6300 Giessen, Federal Republic of Germany

XXIV

Preface

Wir fohlen, daB selbst, wenn aile moglichen wissenschaftlichen Fragen beantwortet sind, unsere Lebensprobleme noch garnicht berilhrt sind.

Ludwig Wittgenstein, Tractus logico-philosophicus (1921), Annalen der Naturphilosophie

In June 1982, physicans and scientists met in Wilsede for the fifth time to discuss origin and treatment ofleukemia.

Lectures and discussions of the meeting closely demonstrated that leukemia can be considered a perfect model for the studies of biolo­gy and treatment of cancer in general. The Wilsede meeting has confirmed that cancer is a multifactorial disease in which oncogenes, regulator proteins, the immunsystem, viruses and chemical noxes play important roles. The development of new revolutionary tech­niques in biological research yielded important insights into the causes of leukemia which are also promising to develop new ways for the treatment of cancer patients.

I am indepted to the chairmen organizing a stimulating and ex­citing program which included all the present and future aspects of human leukemia.

I am grateful to all participating scientists, the supporting stuff and the Wilsede people for making this meeting both scientifically excellent as well as humanly warm and unforgettable. Last but not least, it must be emphazised that the "Verein Naturschutzpark Lii­neburger Heide" and Dr. h.c. Alfred Toepfer are responsible for saving this peaceful place for us.

Hamburg, February 1982 Rolf Neth

xxv

Coffee break and lectures in "De Emmenhoir'. Middle left, Wellcome of Dr. h.c. Alfred Toepfer Down left, Melvin Greaves starting his Stohlman lecture Down right, Donall Thomas giving his Stohlman lecture

XXVI

Personal and scientific discussions in Wilsede, June 1982

(Fotos: Regina Valz, Cetric Parkin)

XXVII

Acknowledgment

We should to thank all who made this workshop possible:

Deutsche Forschungsgemeinschaft Deutsche Krebshilfe Freie und Hansestadt Hamburg Hamburgische Wissenschaftliche Stiftung Leukemia Research Fund, Great Britain Leukemia Society of America Paul Martini Stiftung N iedersachsisches Sozialministeri urn Stifterverband fUr die Deutsche Wissenschaft Universitat Hamburg (Hertha-Grober-Stiftung)

For generous hospitality we thank the Stiftung F. V. S. zu Hamburg, the Amerikahaus in Hamburg, the Freie und Hansestadt Hamburg, and the Hamburger Staatsoper

XXIX