habs state of the science webinar series: habs blooms ...€¦ · habs state of the science webinar...
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HABs State of the Science webinar series:HABs Blooms Detection, Composition & Effects
In partnership with:
July 19, 2016
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Speakers:James Larson – U.S. Geological Survey
Donna Kashian – Wayne State UniversityAli Shakoor – Wayne State UniversityCarrie Givens – U.S. Geological SurveyKevin Meyer – University of Michigan
GoToWebinar Housekeeping Items
• Submit your text questions and comments using the Questions Panel
• Note: This webinar is being recorded and will be posted on the HABs Collaboratory website
July 19, 2016
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Great Lakes HABs Collaboratory“A virtual laboratory for information sharing and collective actions to address
HABs”
Multidisciplinary group, 100+ members from different Agencies, Ministries, Colleges, Universities and Organizations across the Great Lakes
Scientists
Scientists
Managers
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HABs State of the Science webinar series
• Result of the inaugural meeting of the HABs Collaboratory– Identified need for communication between researchers, and between researchers and managers
• Present on‐going research projects related to HABs in the Great Lakes region
• Goals:– Improve communication– Knowledge transfer– Opportunities for collaboration
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Ohio Sea Grant / OSU Stone Lab• Managing 55 HABS related projects (~$7,000,000)
– 18 funded by Ohio Sea Grant– 5 funded by OSU’s Field 2 Faucet initiative– 32 funded under the Ohio Department of Higher Education (OSU/UT; 18
vs. 14)• Stone Lab Guest and Research Lecture Series
– June 16th, 23rd, 30th, July 7th, 14th, 28th, and August 4th– 7pm ‐9pm – https://ohioseagrant.osu.edu/news/calendar
• 9/15/16 “State of Science” meeting in Toledo– Stranahan Theater– Modeling, BMPs, and Public Health‐Water treatment– https://ohioseagrant.osu.edu/news/calendar/2016/09/15/o47km/underst
anding‐algal‐blooms
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James Larson – U.S. Geological Survey
EVALUATING POTENTIAL ECOSYSTEM INDICATORS OF GREAT LAKES RESTORATION SUCCESS
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Project Overview• Evaluating potential ecosystem indicators of
Great Lakes restoration success• James Larson, U.S. Geological Survey• Lake Erie/Saginaw Bay/Traverse Bay (2013‐
2016)• Identify the extent to which gradients in
cyanobacterial blooms, anthropogenic disturbance and other environmental gradients influence biodiversity, secondary production and habitat quality
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Approach
• Ecological Process Monitoring Stations– Common consumer as surrogate for secondary
production– Hester‐Dendy samplers– Decomposition (cotton/metal/plastic)
• Stable isotopes at the base of food webs over time in response to changes in eutrophication
– Archived samples of mayflies in western basin, Lake St. Claire, Detroit river from 1980s‐2014.
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Summary of Findings
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Larson JH, Richardson WB, Evans MA, et al (2016) Measuring spatial variation in secondary production and food quality using a common consumer approach in Lake Erie. Ecol Appl26:873–885.
HABs Collaboratory
• What questions still need to be answered about HABs?
• How can collaboration help your research?
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Donna Kashian, Anna Boegehold, Karim Alame
CYANOBACTERIA LIMITS DREISSENID SPERM MOBILITY AND FERTILIZATION SUCCESS
July 19, 2016
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Project Overview
• Title: Cyanobacteria limits dreissenid sperm mobility and fertilization success
• Investigators: Donna Kashian1, Anna Boegehold1, Karim Alame1, and Nicholas Johnson2
1Wayne State University, 2USGS Hammond Bay Biological Station USGS
• Location: Cyanobacteria cultures collected from water bodies in the Great Lake region; 2013‐present
• Hypothesis: Cyanobacteria inhibits spawning in quagga mussels
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Mussels obtained from the Detroit River, MI were induced to spawn with serotonin for all experiments.
Spawning was assessed when exposed to 13 cyaonbacteriacultures and an isolated toxin (Microcystin‐LR)
Sperm Vitality was assessed by quantifying the distance, velocity, and acceleration of their movements microscopically using image‐tracking software
Fertilization was assessed by combining sperm and eggs in well plates and exposing them to cyanobacteria. Fertilization was monitored microscopically and quantified
Approach
* All experiments had controls of water and/or water with Ankistrodesmus as appropriate.
Summary of Findings
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Dreissenid populations may be particularly sensitive during bloom events when control measures may be more effective‐ better control of mussels potentially better control of blooms‐ feedback mechanisms.
A chemical tool for reducing dreissenid reproduction might be derived from cyanobacteria to be used in tandem with other biocides
What did you learn from your work?Microcystis negatively affects dreissenid reproductionReduced sperm movement, acceleration and decreased fertilization
What happened that you didn’t expect?We did not expect the impacts on sperm; the toxin is not causing the
impacts,
Relevant conclusions for water quality managers?
HABs Collaboratory
What questions still need to be answered about HABs?
How do HABs impact other species?(i.e. Broadcast spawners; fish movement; sub‐lethal impacts etc. )
How can collaboration help your research?Sharing cyanobacteria cultures; biochemist
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Ali Shakoor and Donna Kashian ‐Wayne State University
SPATIAL ECOLOGY AND MOVEMENT OF JUVENILE WALLEYE IN RESPONSE TO HARMFUL ALGAL BLOOMS
July 19, 2016
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Project Overview• Title
– Spatial Ecology and Movement of Juvenile Walleye in Response to Harmful Algal Blooms
• Author & Affiliations– Ali Shakoor and Donna Kashian Wayne St. Univ.
• Funding Source– Erb Family Foundation
• Project Location & Study years– Maumee Bay, Western Basin Lake Erie‐2017 & 18
• Research hypothesis– Juvenile walleye will avoid HABs in Western Basin Lake Erie
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Project background
• What lead you to this project? (past projects, interests, etc.)
– Majority of HAB’s related research was focused on human health, financial effects, water quality, and possible causes.
– An interest in possible food web interactions and trophic dynamics possibly altered by HAB’s
– Interest in walleye biology and ecology
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Approach
• How will you meet your project objectives to answer your hypothesis?
– Track juvenile walleye movements with acoustic telemetry– HAB location, transport, and severity will be tracked as part of GLERL’s existing HABs
program • Statistical analysis of data from current/historical data sets
(ODNR, NOAA, Collaborators)– Water quality, zooplankton abundance and community composition, historical spatial
and abundance patterns of juvenile walleye in response to HAB’s events
• What are your expected results?– We expect that juvenile walleye will avoid HAB’s events– Distinct possibility that they may be attracted to HAB’s events
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Approach
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http://vemco.com/wp‐content/uploads/2013/02/vps_webinar1.htm
HABs Collaboratory• What questions still need to be answered about HABs?
– Are there any behavioral changes to economically/environmentally important species as a result of HAB’s?
– How is a food web already stressed by invasive species further altered by HAB’s events?
– Could HAB’s events have long‐term effects on population dynamics of economically/environmentally important species?
• How can collaboration help your research?– Aquatic invertebrate data– Preyfish abundance/distribution in and around HAB’s– Walleye hatch data– Boat time– Use of any spare Vemco acoustic receivers possible collaborators may have
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Carrie E. Givens*, Joseph W. Duris, Chris D. Ecker, James H. Larson, Keith Loftin, Peter Lenaker, Erin A. Stelzer,
Mary Anne Evans
U.S. Geological [email protected]
CHANGES IN MICROBIAL COMMUNITIES AND MICROBIAL‐MEDIATED PROCESSES IN RELATION TO CYANOBACTERIAL HARMFUL ALGAL BLOOM
FORMATION AND TOXIN PRODUCTION
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This information is preliminary and is subject to revision. It is being provided to meet the need for timely best science. The information is provided on the condition that neither the U.S. Geological Survey nor the U.S. Government may be held liable for any damages resulting from the authorized or unauthorized use of the information.
Project Overview• Landscape to Lake Processes: the Relation of Biotic and
Abiotic Factors to the Development of Harmful Algal Blooms and Their Toxicity
Changes in Microbial Communities and Microbial‐Mediated Processes in Relation to Cyanobacterial Harmful Algal Bloom Formation and Toxin Production
• U.S. Geological Survey – multiple Centers, multiple disciplines
• U.S. EPA Great Lakes Restoration Initiative
• Great Lakes – 2014 to present
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WQ1
WQ2
WQ3
WQ4WQ5W
Q6
Project Objectives– Structural and functional connection between sediment
microbial communities and that of the water column
– Microbial succession prior to, during, and after blooms
– Influence of increased eutrophication on connections between microbial communities of sediment and water column and environmental factors related to HABs
– Affect of cyanobacteria communities on mussel fitness and lipid content
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Project Team• USGS team of scientists
– Microbial ecologist interested in changes in microbial community form and function with environmental perturbations
– Changes in microbial communities in water, sediment, biofilms
– Also interested in host microbiome response – HABs and human and animal health
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Approach• Using next generation sequencing (Illumina MiSeq and
HiSeq) and bioinformatics – 16S rRNA sequencing – “who’s there?”– Metagenomic survey – “who’s there?” and “what
can they do?” (metabolic capabilities)
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HABs Collaboratory
• Influence of sediment cyanobacterial community on next year’s bloom
• Understanding the relation between nutrient type and form on bloom size and toxicity
• Connections between open‐lake and nearshore processes
• Influence of HABs on health and physiology of aquatic organisms
Collaboratory=collaborate– at minimum, know what other scientists are doing, discuss
results
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Kevin A. Meyer – University of Michigan
META‐OMICS ANALYSIS OF LAKE ERIE MICROCYSTIS STRAINS AND BLOOMS
July 19, 2016
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Project Overview• Meta‐omics analysis of Lake Erie Microcystis strains and
blooms• Collaborators
– Kevin A. Meyer – University of Michigan– Greg Dick – University of Michigan– Tim Davis – NOAA: GLERL– Derek Smith – University of Michigan– Sue Watson – Environment Canada
• Funding provided by:– Great Lakes Water Restoration Initiative– UM Water Center
• Lake Erie western basin: 2014 – ongoing
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Approach• Annual sampling
during bloom season– In collaboration
with GLERL sampling
– Toxicity– Metagenomics &
metatranscriptomics• Microcystis sp.• Associated
bacteria
– Hydrogen peroxide
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Summary of Findings• Microcystis has a high proportion of strain‐
specific unique genes– Unique genes are present and expressed in Lake Erie– More defense‐oriented genes in the flexible genes
• The Lake Erie microbial community influences hydrogen peroxide fluxes
– Microcystis contribute to the source but likely not the sink
– Microcystis likely relies on other microbes to combat oxidative stress from hydrogen peroxide
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HABs Collaboratory• What questions still need to be answered about HABs?
– Are unique genes regulated in response to environmental changes?– What role do associated bacteria play in responding to reactive
oxygen species?– Does higher oxidative stress select for toxic strains of Microcystis or
regulate mcy expression. • How can collaboration help your research?
– Exchange new/unique isolates of Microcystis – Exchange sequences from metagenomic and metatranscriptomic
studies of Lake Erie Microcystis blooms– Building a graph database using Neo4j
• Great Lakes microbes• Searchable relationships, gene clusters, and annotations
– Investigate diel cycles of both H2O2 and unique gene expression
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HABs Blooms Detection, Composition & Effects
In partnership with:
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Questions?
Coming up next:HABs & Public Health
Tuesday, July 26 2016, 1:00‐2:00 pm (EDT)
HABs Monitoring & Forecasting – Thursday August 11, 1‐2 p.m. (EDT)HABs Blooms Sources & Toxicity – Tuesday August 16,
11 a.m.‐12 p.m. (EDT)HABs: Educate and Engage ‐ TBD
To learn more about the HABs Collaboratory and the HABs State of the Science Webinar Series, visit us at:
http://glc.org/projects/water‐quality/habs/
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